CN106967552A - A kind of brew method of purple potato Huangguan Pear compound fruit wine - Google Patents
A kind of brew method of purple potato Huangguan Pear compound fruit wine Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
- C12G3/025—Low-alcohol beverages
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Abstract
一种紫薯黄冠梨复合果酒的酿制方法,包括选材和原料预处理、酶解糖化、SO2杀菌、调糖和调酸、制备酵母菌种子液、接种发酵、陈酿发酵、换瓶澄清、成品调配和硅藻土过滤。本发明首先通过淀粉酶对紫薯泥进行酶解后与梨汁混合,然后通过果胶酶和糖化酶一起进行糖化,在适当的酶解、糖化工艺条件下,紫薯泥的酶解出汁率达到了68%以上,紫薯黄冠梨汁的糖化出汁率达到了70%以上;采用库德里阿兹威氏毕赤酵母发酵的方法并配合适当的发酵工艺条件,酿制的紫薯黄冠梨复合果酒酒精度达到了12%vol以上。本发明制得的紫薯黄冠梨复合果酒颜色清澈透明,质地细腻稳定,兼具紫薯和黄冠梨的芬芳果香和营养保健功效,风味独特,圆润爽口,营养丰富。A brewing method of purple sweet potato huangguan pear compound fruit wine, including material selection and raw material pretreatment, enzymatic hydrolysis and saccharification, SO2 sterilization, sugar and acid adjustment, preparation of yeast seed liquid, inoculation and fermentation, aging fermentation, bottle change and clarification , finished product deployment and diatomaceous earth filtration. In the present invention, the mashed purple sweet potato is first enzymolyzed by amylase and then mixed with pear juice, and then saccharified by pectinase and glucoamylase. It has reached more than 68%, and the saccharification juice yield of purple potato Huangguan pear juice has reached more than 70%; the purple potato Huangguan brewed is made by using the fermentation method of Pichia wisteria and the appropriate fermentation process conditions. The alcohol content of the pear compound fruit wine has reached more than 12% vol. The purple sweet potato and yellow crown pear compound fruit wine prepared by the invention has clear and transparent color, delicate and stable texture, has both the fragrant and fruity aroma and nutritional and health care effects of purple sweet potato and yellow crown pear, has unique flavor, is mellow and refreshing, and is rich in nutrition.
Description
技术领域technical field
本发明涉及一种果酒制作技术领域,具体涉及一种紫薯黄冠梨复合果酒的酿制方法。The invention relates to the technical field of fruit wine production, in particular to a brewing method of purple sweet potato, yellow crown and pear compound fruit wine.
背景技术Background technique
果酒是以植物果实为原料经发酵酿制而成的低度饮料酒,与白酒相比,具有低酒度、高营养、益脑健身等特点。Fruit wine is a low-alcohol beverage wine brewed from plant fruits through fermentation. Compared with white wine, it has the characteristics of low alcohol content, high nutrition, and brain fitness.
紫薯又称黑薯,薯肉呈紫至深紫色,除具有普通甘薯所含有的淀粉、膳食纤维、维生素等营养成分外,其矿物质丰富,富含花青素,有较高的药用价值,是一种栽培容易、药食兼用的理想作物。据检测证明,紫薯含有丰富的锌、钙、镁等多种人体有益元素,特别含有丰富的硒元素,硒元素已经被世界医学界称为抗癌之王。而其所富含的花青素已被证明具有提高人体免疫力,抗癌防癌、软化血管、降血压、降血脂等保健作用。Purple potato is also called black potato, and its flesh is purple to deep purple. In addition to the starch, dietary fiber, vitamins and other nutrients contained in ordinary sweet potatoes, it is rich in minerals, rich in anthocyanins, and has high medicinal properties. It is an ideal crop that is easy to cultivate and can be used for both medicine and food. According to test results, purple sweet potato is rich in zinc, calcium, magnesium and other beneficial elements for human body, especially rich in selenium, which has been called the king of anti-cancer by the world medical circle. The anthocyanins it is rich in have been proven to have health effects such as improving human immunity, anti-cancer and anti-cancer, softening blood vessels, lowering blood pressure, and lowering blood lipids.
黄冠梨是以雪花梨和日本新世纪梨杂交培育而成,成熟后的果实果皮为黄色,果面光洁无锈,果点小而密,果肉洁白,肉质细而松脆,汁液含量丰富,风味酸甜适口,是我国梨的主栽品种之一。梨中的营养物质可以使身体细胞保持健康状态,辅助器官排毒、净化身体、软化血管、增进血液循环,并可以把钙质输送到骨骼,加强骨钙质。另外,加热后的梨汁中含有大批的多酚物质,具备良好的抗癌功用。Huangguan pear is a hybrid of snow pear and Japanese new century pear. After ripening, the fruit has yellow skin, smooth and rust-free surface, small and dense fruit spots, white flesh, fine and crunchy flesh, and rich juice content. The flavor is sour and sweet, and it is one of the main varieties of pears in my country. The nutrients in pears can keep body cells in a healthy state, assist organs to detoxify, purify the body, soften blood vessels, improve blood circulation, and transport calcium to bones to strengthen bone calcium. In addition, the heated pear juice contains a lot of polyphenols, which has good anti-cancer properties.
公开号为CN105950341A的专利公开了一种具有营养保健功能的紫薯梨酒的制备方法,该制作方法包括以下步骤:(1)将紫薯打浆,经液化、糖化、取其清汁,得紫薯汁;将梨压榨得到梨汁;(2)将紫薯汁和梨汁混合后装入无菌发酵罐中,并加入活化的酵母进行发酵,然后澄清过滤,再经灌装、灭菌,即得到紫薯梨酒成品。该发明中仅仅对紫薯进行了液化和糖化得到紫薯汁,然后再将紫薯汁与梨汁进行混合后进行无菌发酵,然而,采用该方法紫薯的酶解出汁率不高,制得的紫薯梨复合果酒酒精度不高,且颜色不透明。The patent with the publication number CN105950341A discloses a preparation method of purple sweet potato pear wine with nutritional and health care functions. The preparation method includes the following steps: (1) beating the purple sweet potato, liquefying, saccharifying, and taking its clear juice to obtain the purple sweet potato juice; squeeze pears to obtain pear juice; (2) put purple potato juice and pear juice into a sterile fermenter after mixing, and add activated yeast to ferment, then clarify and filter, and then fill and sterilize. The finished purple potato pear wine was obtained. In this invention, only the purple sweet potato is liquefied and saccharified to obtain purple sweet potato juice, and then the purple sweet potato juice is mixed with pear juice and then aseptically fermented. The obtained purple potato pear compound fruit wine has low alcohol content and opaque color.
发明内容Contents of the invention
本发明的目的在于提供一种紫薯黄冠梨复合果酒的酿制方法,该方法可提高紫薯的酶解出汁率、紫薯黄冠梨汁的糖化出汁率、果酒的酒精度以及果酒的颜色透明度。The object of the present invention is to provide a kind of brewing method of purple sweet potato Huangguan pear compound fruit wine, this method can improve the enzymatic hydrolysis juice yield of purple sweet potato, the saccharification juice yield of purple potato Huangguan pear juice, the alcohol degree of fruit wine and the alcohol content of fruit wine. Color transparency.
为实现上述目的,本发明提供了一种紫薯黄冠梨复合果酒的酿制方法,按以下步骤进行:In order to achieve the above object, the invention provides a kind of brewing method of purple sweet potato huangguan pear compound fruit wine, carry out according to the following steps:
(1)选材和原料预处理:选择优质新鲜紫薯,洗净切块后先后进行护色处理和漂烫处理,捞出冷却后进行打浆得到紫薯泥,备用;将新鲜黄冠梨清洗后去皮去核,然后切块后进行压榨得到梨汁,备用;(1) Material selection and raw material pretreatment: select high-quality fresh purple sweet potatoes, wash and cut into pieces, then carry out color protection treatment and blanching treatment successively, remove and cool, then beat to obtain purple sweet potato puree, and set aside; wash fresh Huangguan pears Peel and remove the core, then cut into pieces and press to obtain pear juice, set aside;
(2)酶解糖化:按紫薯泥与梨汁之间的重量比为1:2~2:1分别称取原料,将紫薯泥置于烧杯中,加入蒸馏水润洗到锥形瓶中制备酶解液,向锥形瓶中加入紫薯泥质量0.1~0.3%的淀粉酶后,将锥形瓶置于45~55℃的水浴锅中,酶解pH控制为5.5~6.5,酶解时间为25~35min;再依次向锥形瓶中加入紫薯泥和梨汁总质量0.1~0.3%的果胶酶与紫薯泥和梨汁总质量0.1~0.3%的糖化酶后,将锥形瓶置于45~55℃的水浴锅中,糖化pH控制为3.5~5.5,糖化时间为85~95min;糖化结束后,进行抽滤,取上部清液即得紫薯黄冠梨汁糖化液,并将紫薯黄冠梨汁糖化液泵入发酵罐中,装量为发酵罐容量的70~80%;(2) Enzymatic hydrolysis and saccharification: Weigh the raw materials respectively according to the weight ratio between the purple potato puree and pear juice is 1:2~2:1, put the purple potato puree in a beaker, add distilled water to rinse it into the conical flask Prepare the enzymatic hydrolysis solution, add 0.1-0.3% of the mass of purple potato mash amylase into the conical flask, place the conical flask in a water bath at 45-55°C, control the enzymatic hydrolysis pH to 5.5-6.5, and enzymatic hydrolysis The time is 25~35min; After adding 0.1~0.3% pectinase of purple potato puree and pear juice total mass and 0.1~0.3% glucoamylase of purple potato puree and pear juice total mass in the Erlenmeyer flask successively, the conical The shaped bottle is placed in a water bath at 45-55°C, the saccharification pH is controlled at 3.5-5.5, and the saccharification time is 85-95 minutes; after the saccharification is completed, suction filtration is performed, and the supernatant liquid is obtained to obtain the saccharification liquid of purple potato Huangguan pear juice , and pump the saccharification liquid of purple sweet potato and yellow crown pear juice into the fermenter, and the loading is 70-80% of the capacity of the fermenter;
(3)SO2杀菌:按紫薯黄冠梨汁糖化液与亚硫酸之间1/1000~1/900的重量比例向发酵罐中加入浓度5~8%的亚硫酸;( 3 ) SO2 Sterilization: add the sulfurous acid of concentration 5~8% in fermenter by the weight ratio of 1/1000~1/900 between the saccharified liquid of purple sweet potato Huangguan pear juice and sulfurous acid;
(4)调糖和调酸:测定紫薯黄冠梨汁糖化液的含糖量和含酸量,当含糖量不足时用砂糖调整紫薯黄冠梨汁糖化液的糖度为20~25%;当含酸量不足时通过添加柠檬酸,使紫薯黄冠梨汁糖化液的最终含酸量达到8~12g/L;(4) Adjusting sugar and acidity: measure the sugar content and acid content of the saccharified liquid of purple sweet potato, Huangguan and pear juice. %; when the acid content is insufficient, by adding citric acid, the final acid content of the saccharification liquid of purple sweet potato and pear juice reaches 8-12g/L;
(5)制备酵母菌种子液:筛选具有高乙醇耐受性、高糖耐受性、高渗透耐受性、酸碱耐受性以及高SO2耐受性的库德里阿兹威氏毕赤酵母,进行扩大培养,即得酵母菌种子液;(5) Preparation of yeast seed liquid: Screening of Kudriaz Pichia wischler with high ethanol tolerance, high sugar tolerance, high osmotic tolerance, acid - base tolerance and high SO2 tolerance Yeast is expanded and cultivated to obtain yeast seed liquid;
(6)将酵母菌种子液进行离心得到固体物质,离心条件:转速4000r/min、离心温度为4℃、离心时间1min,再将固体物质通过9%的生理盐水进行多次洗脱后重悬;(6) Centrifuge the yeast seed liquid to obtain solid matter, centrifugation conditions: rotation speed 4000r/min, centrifugation temperature 4°C, centrifugation time 1min, and then resuspend the solid matter through 9% normal saline for multiple elution ;
(7)接种发酵:按发酵罐中紫薯黄冠梨汁量的3~6%(v/v),将经过步骤(6)处理后的酵母菌种子液接种到经过步骤(4)处理后的紫薯黄冠梨汁糖化液中进行主发酵,主发酵采取密闭式发酵,发酵时间11~14天,发酵温度控制在28℃,在发酵高峰期控制温度不超过25℃;(7) inoculation fermentation: press 3~6% (v/v) of purple sweet potato huangguan pear juice amount in the fermentation tank, inoculate the yeast seed liquid after step (6) processing to after step (4) processing The main fermentation is carried out in the saccharification liquid of purple sweet potato Huangguan pear juice. The main fermentation adopts closed fermentation. The fermentation time is 11 to 14 days. The fermentation temperature is controlled at 28°C, and the temperature is controlled not to exceed 25°C during the peak fermentation period;
(8)陈酿发酵:主发酵结束后进行第1次换瓶,换瓶后进行7~10天的后发酵;(8) Aging fermentation: After the main fermentation, the bottle is changed for the first time, and post-fermentation is carried out for 7 to 10 days after the bottle is changed;
(9)换瓶澄清:后发酵后隔4~6天换1次瓶,再每隔10~15天后换瓶2~3次;换瓶时采用虹吸法,不能搅动底层沉淀;经过换瓶后,若复合果酒的透明度仍不够,通过添加明胶和单宁澄清;澄清完成后过滤得到初酒,将初酒密封后于10℃以下陈酿至少6个月;(9) Re-bottle clarification: change the bottle once every 4-6 days after post-fermentation, and then change the bottle 2-3 times after every 10-15 days; , if the transparency of the compound fruit wine is still not enough, clarify by adding gelatin and tannin; after the clarification is completed, filter to obtain the first wine, seal the first wine and age it below 10°C for at least 6 months;
(10)成品调配:陈酿完成后检测初酒的酒精度、酸度、糖度、微生物含量和稳定性;根据NY/T 1508-2007绿色果酒标准进行,分别以酒精度(20℃)为7-18%、酸(以酒石酸计)为4.0~9.0g/L、半干型总糖4.1~12.0g/L的标准对成品进行调配;当酒精度没达标时,通过添加酒精对酒精度进行调整,当酸度不足时,通过添加柠檬酸对酸度进行调整,当糖度不足时,通过添加黄冠梨的浓缩汁对糖度进行调整;(10) Blending of finished products: After the aging is completed, the alcohol content, acidity, sugar content, microbial content and stability of the initial wine are tested; according to the NY/T 1508-2007 green fruit wine standard, the alcohol content (20°C) is 7-18 %, acid (calculated as tartaric acid) is 4.0-9.0g/L, and the standard of semi-dry total sugar is 4.1-12.0g/L to deploy the finished product; when the alcohol content is not up to the standard, the alcohol content is adjusted by adding alcohol, When the acidity is insufficient, the acidity is adjusted by adding citric acid, and when the sugar content is insufficient, the sugar content is adjusted by adding the concentrated juice of Huangguan pear;
(11)硅藻土过滤:将调配好的紫薯黄冠梨复合果酒进行硅藻土过滤,瓶装后进行巴氏灭菌,最后将紫薯黄冠梨复合果酒进行无菌玻璃瓶灌装。(11) Diatomite filtration: the prepared purple sweet potato, Huangguan pear compound fruit wine is filtered with diatomaceous earth, pasteurized after bottled, and finally the purple sweet potato, Huangguan pear compound fruit wine is filled in aseptic glass bottles.
优选的,所述步骤(1)中护色处理为将紫薯块放入1%的柠檬酸亚锡二钠溶液中浸泡4~6min。Preferably, the color protection treatment in the step (1) is soaking the purple potato pieces in 1% disodium stannous citrate solution for 4-6 minutes.
优选的,所述步骤(1)中漂烫处理是将经过护色处理后的紫薯块切成片状后放入95~100℃的热水中漂烫9~10min。Preferably, the blanching treatment in the step (1) is to cut the purple potato pieces that have undergone the color protection treatment into slices and put them into hot water at 95-100° C. for 9-10 minutes.
优选的,所述步骤(2)中紫薯酶解的工艺条件为:向锥形瓶中加入紫薯泥质量0.1%的淀粉酶后,将锥形瓶置于50℃的水浴锅中,酶解pH控制为6.0,酶解时间为30min。Preferably, the process conditions for the enzymatic hydrolysis of purple sweet potato in the step (2) are as follows: after adding 0.1% amylase of purple sweet potato puree mass to the conical flask, place the conical flask in a water bath at 50°C, and the enzymatic The hydrolysis pH was controlled at 6.0, and the enzymatic hydrolysis time was 30 minutes.
优选的,所述步骤(2)中紫薯黄冠梨混合果汁液糖化的工艺条件为:紫薯泥和梨果汁总质量0.10%的果胶酶与紫薯泥和梨果汁总质量0.10%的糖化酶后,将锥形瓶置于50℃的水浴锅中,糖化pH控制为4.0,糖化时间为90min。Preferably, the process conditions for saccharification of purple sweet potato and pear juice mixed fruit juice in the step (2) are: 0.10% pectinase of the total mass of purple potato puree and pear juice and 0.10% of the total mass of purple potato puree and pear juice After the saccharification enzyme, the Erlenmeyer flask was placed in a water bath at 50°C, the saccharification pH was controlled at 4.0, and the saccharification time was 90 minutes.
优选的,所述步骤(3)中紫薯黄冠梨汁糖化液与亚硫酸之间重量比例为1/950,亚硫酸的浓度为6%。Preferably, in the step (3), the weight ratio between the saccharified liquid of purple sweet potato huangguan pear juice and sulfurous acid is 1/950, and the concentration of sulfurous acid is 6%.
与现有技术相比,本发明首先通过淀粉酶对紫薯泥进行酶解,再与梨汁混合,然后通过果胶酶和糖化酶对紫薯黄冠梨汁一起进行糖化,在适当的酶解、糖化工艺条件下,紫薯泥的酶解出汁率达到了68%以上,紫薯黄冠梨汁的糖化出汁率达到了70%以上;采用库德里阿兹威氏毕赤酵母发酵的方法并配合适当的发酵工艺条件,所酿制的紫薯黄冠梨复合果酒酒精度达到了12%vol以上。本发明所制得的紫薯黄冠梨复合果酒颜色清澈透明,质地细腻、稳定,兼具紫薯和黄冠梨的芬芳果香和营养保健功效,酒体丰满,风味独特,圆润爽口,口感醇厚,营养丰富,且酿制成本经济,可实现规模化生产。Compared with the prior art, the present invention first uses amylase to enzymolyze the purple potato mash, then mixes it with pear juice, and then uses pectinase and glucoamylase to saccharify the purple potato and Huangguan pear juice together. Under the condition of hydrolysis and saccharification process, the enzymatic hydrolysis juice yield of purple potato puree reached more than 68%, and the saccharification juice yield of purple potato Huangguan pear juice reached more than 70%; And with proper fermentation process conditions, the alcohol content of the brewed purple sweet potato and yellow crown pear compound fruit wine has reached more than 12% vol. The purple sweet potato and yellow crown pear compound fruit wine prepared by the present invention is clear and transparent in color, delicate and stable in texture, has both the fragrance and fruity aroma and nutritional and health care effects of purple sweet potato and yellow crown pear, and has a full body, unique flavor, round and refreshing taste, and It is mellow and nutritious, and the brewing cost is economical, which can realize large-scale production.
具体实施方式detailed description
以下结合实施例对本发明作进一步详细说明。The present invention is described in further detail below in conjunction with embodiment.
实施例一Embodiment one
一种紫薯黄冠梨复合果酒的酿制方法,按以下步骤进行:A kind of brewing method of purple sweet potato yellow crown pear compound fruit wine is carried out according to the following steps:
(1)选材和原料预处理:选择优质新鲜紫薯,洗净切块后先后放入1%的柠檬酸亚锡二钠溶液中浸泡4min进行护色处理,将经过护色处理后的紫薯块切成片状后放入95℃的热水中漂烫10min,捞出冷却后进行打浆得到紫薯泥,备用;将新鲜黄冠梨清洗后去皮去核,然后切块后进行压榨得到梨汁,备用;(1) Material selection and raw material pretreatment: select high-quality fresh purple sweet potatoes, wash and cut into pieces, soak in 1% stannous disodium citrate solution for 4 minutes for color protection treatment, and put the purple sweet potatoes after color protection treatment Cut the pieces into slices, put them in hot water at 95°C for 10 minutes, remove them and cool them down, and beat them to obtain purple sweet potato mash, which will be used for later use; wash the fresh Huangguan pears, peel and remove the core, cut them into pieces, and press them to obtain mashed potatoes. pear juice, set aside;
(2)酶解糖化:按紫薯泥与梨汁之间的重量比为1:2分别称取原料,将紫薯泥置于烧杯中,加入蒸馏水润洗到锥形瓶中制备酶解液,向锥形瓶中加入紫薯泥质量0.3%的淀粉酶后,将锥形瓶置于45℃的水浴锅中,酶解pH控制为5.5,酶解时间为35min;再依次向锥形瓶中加入紫薯泥和梨汁总质量0.3%的果胶酶与紫薯泥和梨汁总质量0.3%的糖化酶后,将锥形瓶置于45℃的水浴锅中,糖化pH控制为3.5,糖化时间为95min;糖化结束后,进行抽滤,取上部清液即得紫薯黄冠梨汁糖化液,并将紫薯黄冠梨汁糖化液泵入发酵罐中,装量为发酵罐容量的70~80%;(2) Enzymatic hydrolysis and saccharification: Weigh the raw materials according to the weight ratio of purple sweet potato puree and pear juice as 1:2, put the purple sweet potato puree in a beaker, add distilled water to rinse and wash it into a conical flask to prepare an enzymatic hydrolysis solution , after adding 0.3% amylase of purple potato puree mass to the Erlenmeyer flask, place the Erlenmeyer flask in a water bath at 45°C, control the enzymolysis pH to 5.5, and the enzymolysis time is 35min; After adding 0.3% pectinase and 0.3% glucoamylase in the total mass of purple potato puree and pear juice, the Erlenmeyer flask was placed in a water bath at 45°C, and the saccharification pH was controlled at 3.5 , the saccharification time is 95min; after the saccharification is completed, suction filtration is carried out, and the supernatant liquid is taken to obtain the saccharified liquid of purple sweet potato and yellow crown pear juice, and the saccharified liquid of purple potato and yellow crown pear juice is pumped into the fermenter, and the capacity of the fermenter is 70-80% of capacity;
(3)SO2杀菌:按紫薯黄冠梨汁糖化液与亚硫酸之间1/1000的重量比例向发酵罐中加入浓度8%的亚硫酸;( 3 ) SO Sterilization: add the sulfurous acid of concentration 8% in the fermenter by the weight ratio of 1/1000 between the saccharified liquid of purple sweet potato Huangguan pear juice and sulfurous acid;
(4)调糖和调酸:测定紫薯黄冠梨汁糖化液的含糖量和含酸量,当含糖量不足时用砂糖调整紫薯黄冠梨汁糖化液的糖度为20~25%;当含酸量不足时通过添加柠檬酸,使紫薯黄冠梨汁糖化液的最终含酸量达到8~12g/L;(4) Adjusting sugar and acidity: measure the sugar content and acid content of the saccharified liquid of purple sweet potato, Huangguan and pear juice. %; when the acid content is insufficient, by adding citric acid, the final acid content of the saccharification liquid of purple sweet potato and pear juice reaches 8-12g/L;
(5)制备酵母菌种子液:筛选具有高乙醇耐受性、高糖耐受性、高渗透耐受性、酸碱耐受性以及高SO2耐受性的库德里阿兹威氏毕赤酵母,进行扩大培养,即得酵母菌种子液;(5) Preparation of yeast seed liquid: Screening of Kudriaz Pichia wischler with high ethanol tolerance, high sugar tolerance, high osmotic tolerance, acid - base tolerance and high SO2 tolerance Yeast is expanded and cultivated to obtain yeast seed liquid;
(6)将酵母菌种子液进行离心得到固体物质,离心条件:转速4000r/min、离心温度为4℃、离心时间1min,再将固体物质通过9%的生理盐水进行多次洗脱后重悬;(6) Centrifuge the yeast seed liquid to obtain solid matter, centrifugation conditions: rotation speed 4000r/min, centrifugation temperature 4°C, centrifugation time 1min, and then resuspend the solid matter through 9% normal saline for multiple elution ;
(7)接种发酵:按发酵罐中紫薯黄冠梨汁量的3~6%(v/v),将经过步骤(6)处理后的酵母菌种子液接种到经过步骤(4)处理后的紫薯黄冠梨汁糖化液中进行主发酵,主发酵采取密闭式发酵,发酵时间14天,发酵温度控制在28℃,在发酵高峰期控制温度不超过25℃;(7) inoculation fermentation: press 3~6% (v/v) of purple sweet potato huangguan pear juice amount in the fermentation tank, inoculate the yeast seed liquid after step (6) processing to after step (4) processing The main fermentation is carried out in the saccharification liquid of purple sweet potato Huangguan pear juice. The main fermentation adopts closed fermentation. The fermentation time is 14 days. The fermentation temperature is controlled at 28 ° C, and the temperature is controlled not to exceed 25 ° C during the peak fermentation period;
(8)陈酿发酵:主发酵结束后进行第1次换瓶,换瓶后进行10天的后发酵;(8) Aging fermentation: After the main fermentation, the bottle is changed for the first time, and after the bottle is changed, post-fermentation is carried out for 10 days;
(9)换瓶澄清:后发酵后隔4~6天换1次瓶,再每隔10~15天后换瓶2~3次;换瓶时采用虹吸法,不能搅动底层沉淀;经过换瓶后,若复合果酒的透明度仍不够,通过添加明胶和单宁澄清;澄清完成后过滤得到初酒,将初酒密封后于10℃以下陈酿至少6个月;(9) Re-bottle clarification: change the bottle once every 4-6 days after post-fermentation, and then change the bottle 2-3 times after every 10-15 days; , if the transparency of the compound fruit wine is still not enough, clarify by adding gelatin and tannin; after the clarification is completed, filter to obtain the first wine, seal the first wine and age it below 10°C for at least 6 months;
(10)成品调配:陈酿完成后检测初酒的酒精度为12%、酸(以酒石酸计)为5.4g/L、糖度为4.8g/L,微生物含量和稳定性均符合NY/T 1508-2007绿色果酒标准;(10) Blending of finished products: After the aging is completed, the alcohol content of the initial wine is 12%, the acid (calculated as tartaric acid) is 5.4g/L, and the sugar content is 4.8g/L. The microbial content and stability are in line with NY/T 1508- 2007 Green Fruit Wine Standard;
(11)硅藻土过滤:将调配好的紫薯黄冠梨复合果酒进行硅藻土过滤,瓶装后进行巴氏灭菌,最后将紫薯黄冠梨复合果酒进行无菌玻璃瓶灌装。(11) Diatomite filtration: the prepared purple sweet potato, Huangguan pear compound fruit wine is filtered with diatomaceous earth, pasteurized after bottled, and finally the purple sweet potato, Huangguan pear compound fruit wine is filled in aseptic glass bottles.
该复合果酒颜色清澈透明,质地细腻、稳定,兼具紫薯和黄冠梨的芬芳果香,酒体丰满,风味独特,圆润爽口,口感醇厚,营养丰富,卫生指标符合NY/T 1508-2007绿色果酒标准的规定。经检测,本实施例中紫薯泥的酶解出汁率达到72.5%,紫薯黄冠梨汁的糖化出汁率达到75.8%。The compound fruit wine is clear and transparent in color, delicate and stable in texture, and has the aroma and fruity aroma of purple sweet potato and yellow crown pear. Provisions of the Green Fruit Wine Standard. After testing, in this embodiment, the enzymatic hydrolysis yield of purple potato puree reaches 72.5%, and the saccharification yield of purple sweet potato Huangguan pear juice reaches 75.8%.
实施例二Embodiment two
一种紫薯黄冠梨复合果酒的酿制方法,按以下步骤进行:A kind of brewing method of purple sweet potato yellow crown pear compound fruit wine is carried out according to the following steps:
(1)选材和原料预处理:选择优质新鲜紫薯,洗净切块后先后放入1%的柠檬酸亚锡二钠溶液中浸泡5min进行护色处理,将经过护色处理后的紫薯块切成片状后放入98℃的热水中漂烫9.5min,捞出冷却后进行打浆得到紫薯泥,备用;将新鲜黄冠梨清洗后去皮去核,然后切块后进行压榨得到梨汁,备用;(1) Material selection and raw material pretreatment: select high-quality fresh purple sweet potatoes, wash and cut into pieces, soak them in 1% disodium stannous citrate solution for 5 minutes for color protection treatment, and put the purple sweet potatoes after color protection treatment Cut the pieces into slices and put them in hot water at 98°C for 9.5 minutes, remove and cool, and beat to obtain purple sweet potato mash for later use; wash the fresh Huangguan pears, peel and remove the core, then cut into pieces and press Get pear juice and set aside;
(2)酶解糖化:按紫薯泥与梨汁之间的重量比为3:2分别称取原料,将紫薯泥置于烧杯中,加入蒸馏水润洗到锥形瓶中制备酶解液,向锥形瓶中加入紫薯泥质量0.1%的淀粉酶后,将锥形瓶置于50℃的水浴锅中,酶解pH控制为6.0,酶解时间为30min;再依次向锥形瓶中加入紫薯泥和梨汁总质量0.1%的果胶酶与紫薯泥和梨汁总质量0.1%的糖化酶后,将锥形瓶置于50℃的水浴锅中,糖化pH控制为4.0,糖化时间为90min;糖化结束后,进行抽滤,取上部清液即得紫薯黄冠梨汁糖化液,并将紫薯黄冠梨汁糖化液泵入发酵罐中,装量为发酵罐容量的70~80%;(2) Enzymatic hydrolysis and saccharification: Weigh the raw materials according to the weight ratio between the purple potato puree and pear juice as 3:2, put the purple potato puree in a beaker, add distilled water to rinse it into a conical flask to prepare an enzymatic hydrolysis solution , after adding 0.1% amylase from purple potato puree to the Erlenmeyer flask, place the Erlenmeyer flask in a water bath at 50°C, control the enzymolysis pH to 6.0, and enzymolysis time is 30min; After adding 0.1% pectinase and 0.1% glucoamylase in the total mass of purple potato puree and pear juice, the Erlenmeyer flask was placed in a water bath at 50°C, and the saccharification pH was controlled at 4.0 , the saccharification time is 90 minutes; after the saccharification is completed, suction filtration is carried out, and the supernatant liquid is taken to obtain the saccharified liquid of purple potato, Huangguan and pear juice, and the saccharified liquid of purple potato, Huangguan and pear juice is pumped into the fermenter, and the capacity of the fermenter is 70-80% of capacity;
(3)SO2杀菌:按紫薯黄冠梨汁糖化液与亚硫酸之间1/950的重量比例向发酵罐中加入浓度6%的亚硫酸;( 3 ) SO Sterilization: add the sulfurous acid of concentration 6% in fermenter by the weight ratio of 1/950 between purple sweet potato Huangguan pear juice saccharification liquid and sulfurous acid;
(4)调糖和调酸:测定紫薯黄冠梨汁糖化液的含糖量和含酸量,当含糖量不足时用砂糖调整紫薯黄冠梨汁糖化液的糖度为20~25%;当含酸量不足时通过添加柠檬酸,使紫薯黄冠梨汁糖化液的最终含酸量达到8~12g/L;(4) Adjusting sugar and acidity: measure the sugar content and acid content of the saccharified liquid of purple sweet potato, Huangguan and pear juice. %; when the acid content is insufficient, by adding citric acid, the final acid content of the saccharification liquid of purple sweet potato and pear juice reaches 8-12g/L;
(5)制备酵母菌种子液:筛选具有高乙醇耐受性、高糖耐受性、高渗透耐受性、酸碱耐受性以及高SO2耐受性的库德里阿兹威氏毕赤酵母,进行扩大培养,即得酵母菌种子液;(5) Preparation of yeast seed liquid: Screening of Kudriaz Pichia wischler with high ethanol tolerance, high sugar tolerance, high osmotic tolerance, acid - base tolerance and high SO2 tolerance Yeast is expanded and cultivated to obtain yeast seed liquid;
(6)将酵母菌种子液进行离心得到固体物质,离心条件:转速4000r/min、离心温度为4℃、离心时间1min,再将固体物质通过9%的生理盐水进行多次洗脱后重悬;(6) Centrifuge the yeast seed liquid to obtain solid matter, centrifugation conditions: rotation speed 4000r/min, centrifugation temperature 4°C, centrifugation time 1min, and then resuspend the solid matter through 9% normal saline for multiple elution ;
(7)接种发酵:按发酵罐中紫薯黄冠梨汁量的3~6%(v/v),将经过步骤(6)处理后的酵母菌种子液接种到经过步骤(4)处理后的紫薯黄冠梨汁糖化液中进行主发酵,主发酵采取密闭式发酵,发酵时间11天,发酵温度控制在28℃,在发酵高峰期控制温度不超过25℃;(7) inoculation fermentation: press 3~6% (v/v) of purple sweet potato huangguan pear juice amount in the fermentation tank, inoculate the yeast seed liquid after step (6) processing to after step (4) processing The main fermentation is carried out in the saccharification liquid of purple sweet potato Huangguan pear juice. The main fermentation adopts closed fermentation. The fermentation time is 11 days. The fermentation temperature is controlled at 28 ° C, and the temperature is controlled not to exceed 25 ° C during the peak fermentation period;
(8)陈酿发酵:主发酵结束后进行第1次换瓶,换瓶后进行7天的后发酵;(8) Aging fermentation: After the main fermentation, the bottle is changed for the first time, and after the bottle is changed, post-fermentation is carried out for 7 days;
(9)换瓶澄清:后发酵后隔4~6天换1次瓶,再每隔10~15天后换瓶2~3次;换瓶时采用虹吸法,不能搅动底层沉淀;经过换瓶后,若复合果酒的透明度仍不够,通过添加明胶和单宁澄清;澄清完成后过滤得到初酒,将初酒密封后于10℃以下陈酿至少6个月;(9) Re-bottle clarification: change the bottle once every 4-6 days after post-fermentation, and then change the bottle 2-3 times after every 10-15 days; , if the transparency of the compound fruit wine is still not enough, clarify by adding gelatin and tannin; after the clarification is completed, filter to obtain the first wine, seal the first wine and age it below 10°C for at least 6 months;
(10)成品调配:陈酿完成后检测初酒的酒精度为14%、酸(以酒石酸计)为6.8g/L、糖度为7.5g/L,微生物含量和稳定性均符合NY/T 1508-2007绿色果酒标准;(10) Blending of finished products: After the aging is completed, the alcohol content of the initial wine is 14%, the acid (calculated as tartaric acid) is 6.8g/L, and the sugar content is 7.5g/L. The microbial content and stability are in line with NY/T 1508- 2007 Green Fruit Wine Standard;
(11)硅藻土过滤:将调配好的紫薯黄冠梨复合果酒进行硅藻土过滤,瓶装后进行巴氏灭菌,最后将紫薯黄冠梨复合果酒进行无菌玻璃瓶灌装。(11) Diatomite filtration: the prepared purple sweet potato, Huangguan pear compound fruit wine is filtered with diatomaceous earth, pasteurized after bottled, and finally the purple sweet potato, Huangguan pear compound fruit wine is filled in aseptic glass bottles.
该复合果酒颜色清澈透明,质地细腻、稳定,兼具紫薯和黄冠梨的芬芳果香,酒体丰满,风味独特,圆润爽口,口感醇厚,营养丰富,卫生指标符合NY/T 1508-2007绿色果酒标准的规定。经检测,本实施例中紫薯泥的酶解出汁率达到75.5%,紫薯黄冠梨汁的糖化出汁率达到73.6%。The compound fruit wine is clear and transparent in color, delicate and stable in texture, and has the aroma and fruity aroma of purple sweet potato and yellow crown pear. Provisions of the Green Fruit Wine Standard. After testing, in this embodiment, the enzymatic hydrolysis yield of purple potato puree reaches 75.5%, and the saccharification yield of purple potato Huangguan pear juice reaches 73.6%.
实施例三Embodiment three
一种紫薯黄冠梨复合果酒的酿制方法,按以下步骤进行:A kind of brewing method of purple sweet potato yellow crown pear compound fruit wine is carried out according to the following steps:
(1)选材和原料预处理:选择优质新鲜紫薯,洗净切块后先后放入1%的柠檬酸亚锡二钠溶液中浸泡6min进行护色处理,将经过护色处理后的紫薯块切成片状后放入100℃的热水中漂烫9min,捞出冷却后进行打浆得到紫薯泥,备用;将新鲜黄冠梨清洗后去皮去核,然后切块后进行压榨得到梨汁,备用;(1) Material selection and raw material pretreatment: select high-quality fresh purple sweet potatoes, wash and cut into pieces, soak in 1% stannous disodium citrate solution for 6 minutes for color protection treatment, and put the purple sweet potatoes after color protection treatment Cut the pieces into slices, put them into hot water at 100°C for 9 minutes, take them out and cool them down, and beat them to obtain purple potato mash, which will be used for later use; wash the fresh Huangguan pears, peel and remove the core, then cut into pieces and press to obtain pear juice, set aside;
(2)酶解糖化:按紫薯泥与梨汁之间的重量比为2:1分别称取原料,将紫薯泥置于烧杯中,加入蒸馏水润洗到锥形瓶中制备酶解液,向锥形瓶中加入紫薯泥质量0.2%的淀粉酶后,将锥形瓶置于55℃的水浴锅中,酶解pH控制为6.5,酶解时间为25min;再依次向锥形瓶中加入紫薯泥和梨汁总质量0.2%的果胶酶与紫薯泥和梨汁总质量0.2%的糖化酶后,将锥形瓶置于55℃的水浴锅中,糖化pH控制为5.5,糖化时间为85min;糖化结束后,进行抽滤,取上部清液即得紫薯黄冠梨汁糖化液,并将紫薯黄冠梨汁糖化液泵入发酵罐中,装量为发酵罐容量的70~80%;(2) Enzymatic hydrolysis and saccharification: Weigh the raw materials respectively according to the weight ratio between the purple sweet potato puree and pear juice as 2:1, put the purple sweet potato puree in a beaker, add distilled water to rinse and wash it into a conical flask to prepare an enzymatic hydrolysis solution , after adding 0.2% amylase of purple potato puree mass to the Erlenmeyer flask, place the Erlenmeyer flask in a water bath at 55°C, control the enzymolysis pH to 6.5, and enzymolysis time is 25min; After adding 0.2% pectinase and 0.2% glucoamylase in the total mass of purple potato puree and pear juice, the Erlenmeyer flask was placed in a water bath at 55°C, and the saccharification pH was controlled at 5.5 , the saccharification time is 85min; after the saccharification is completed, suction filtration is carried out, and the supernatant liquid is taken to obtain the saccharified liquid of purple sweet potato and yellow crown pear juice, and the saccharified liquid of purple potato and yellow crown pear juice is pumped into the fermenter, and the capacity of the fermenter is 70-80% of capacity;
(3)SO2杀菌:按紫薯黄冠梨汁糖化液与亚硫酸之间1/900的重量比例向发酵罐中加入浓度6%的亚硫酸;( 3 ) SO Sterilization: add the sulfurous acid of concentration 6% in fermenter by the weight ratio of 1/900 between the saccharified liquid of purple potato Huangguan pear juice and sulfurous acid;
(4)调糖和调酸:测定紫薯黄冠梨汁糖化液的含糖量和含酸量,当含糖量不足时用砂糖调整紫薯黄冠梨汁糖化液的糖度为20~25%;当含酸量不足时通过添加柠檬酸,使紫薯黄冠梨汁糖化液的最终含酸量达到8~12g/L;(4) Adjusting sugar and acidity: measure the sugar content and acid content of the saccharified liquid of purple sweet potato, Huangguan and pear juice. %; when the acid content is insufficient, by adding citric acid, the final acid content of the saccharification liquid of purple sweet potato and pear juice reaches 8-12g/L;
(5)制备酵母菌种子液:筛选具有高乙醇耐受性、高糖耐受性、高渗透耐受性、酸碱耐受性以及高SO2耐受性的库德里阿兹威氏毕赤酵母,进行扩大培养,即得酵母菌种子液;(5) Preparation of yeast seed liquid: Screening of Kudriaz Pichia wischler with high ethanol tolerance, high sugar tolerance, high osmotic tolerance, acid - base tolerance and high SO2 tolerance Yeast is expanded and cultivated to obtain yeast seed liquid;
(6)将酵母菌种子液进行离心得到固体物质,离心条件:转速4000r/min、离心温度为4℃、离心时间1min,再将固体物质通过9%的生理盐水进行多次洗脱后重悬;(6) Centrifuge the yeast seed liquid to obtain solid matter, centrifugation conditions: rotation speed 4000r/min, centrifugation temperature 4°C, centrifugation time 1min, and then resuspend the solid matter through 9% normal saline for multiple elution ;
(7)接种发酵:按发酵罐中紫薯黄冠梨汁量的3~6%(v/v),将经过步骤(6)处理后的酵母菌种子液接种到经过步骤(4)处理后的紫薯黄冠梨汁糖化液中进行主发酵,主发酵采取密闭式发酵,发酵时间13天,发酵温度控制在28℃,在发酵高峰期控制温度不超过25℃;(7) inoculation fermentation: press 3~6% (v/v) of purple sweet potato huangguan pear juice amount in the fermentation tank, inoculate the yeast seed liquid after step (6) processing to after step (4) processing The main fermentation is carried out in the saccharification liquid of purple sweet potato Huangguan pear juice. The main fermentation adopts closed fermentation. The fermentation time is 13 days.
(8)陈酿发酵:主发酵结束后进行第1次换瓶,换瓶后进行8天的后发酵;(8) Aging fermentation: After the main fermentation, the bottle is changed for the first time, and after the bottle is changed, post-fermentation is carried out for 8 days;
(9)换瓶澄清:后发酵后隔4~6天换1次瓶,再每隔10~15天后换瓶2~3次;换瓶时采用虹吸法,不能搅动底层沉淀;经过换瓶后,若复合果酒的透明度仍不够,通过添加明胶和单宁澄清;澄清完成后过滤得到初酒,将初酒密封后于10℃以下陈酿至少6个月;(9) Re-bottle clarification: change the bottle once every 4-6 days after post-fermentation, and then change the bottle 2-3 times after every 10-15 days; , if the transparency of the compound fruit wine is still not enough, clarify by adding gelatin and tannin; after the clarification is completed, filter to obtain the first wine, seal the first wine and age it below 10°C for at least 6 months;
(10)成品调配:陈酿完成后检测初酒的酒精度为15%、酸(以酒石酸计)为6.4g/L、糖度为6.2g/L,微生物含量和稳定性均符合NY/T 1508-2007绿色果酒标准;(10) Blending of finished products: After the aging is completed, the alcohol content of the initial wine is 15%, the acid (calculated as tartaric acid) is 6.4g/L, and the sugar content is 6.2g/L. The microbial content and stability are in line with NY/T 1508- 2007 Green Fruit Wine Standard;
(11)硅藻土过滤:将调配好的紫薯黄冠梨复合果酒进行硅藻土过滤,瓶装后进行巴氏灭菌,最后将紫薯黄冠梨复合果酒进行无菌玻璃瓶灌装。(11) Diatomite filtration: the prepared purple sweet potato, Huangguan pear compound fruit wine is filtered with diatomaceous earth, pasteurized after bottled, and finally the purple sweet potato, Huangguan pear compound fruit wine is filled in aseptic glass bottles.
该复合果酒颜色清澈透明,质地细腻、稳定,兼具紫薯和黄冠梨的芬芳果香,酒体丰满,风味独特,圆润爽口,口感醇厚,营养丰富,卫生指标符合NY/T 1508-2007绿色果酒标准的规定。经检测,本实施例中紫薯泥的酶解出汁率达到74.2%,紫薯黄冠梨汁的糖化出汁率达到74.1%。The compound fruit wine is clear and transparent in color, delicate and stable in texture, and has the aroma and fruity aroma of purple sweet potato and yellow crown pear. Provisions of the Green Fruit Wine Standard. After testing, in this embodiment, the enzymatic hydrolysis yield of purple potato puree reaches 74.2%, and the saccharification yield of purple potato Huangguan pear juice reaches 74.1%.
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