CN106957800A - The cultural method of COD degradation bacterium - Google Patents

The cultural method of COD degradation bacterium Download PDF

Info

Publication number
CN106957800A
CN106957800A CN201710248049.2A CN201710248049A CN106957800A CN 106957800 A CN106957800 A CN 106957800A CN 201710248049 A CN201710248049 A CN 201710248049A CN 106957800 A CN106957800 A CN 106957800A
Authority
CN
China
Prior art keywords
degradation bacterium
cod degradation
parts
test tube
powers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710248049.2A
Other languages
Chinese (zh)
Inventor
张峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xi'an Huanuo Environmental Protection Co Ltd
Original Assignee
Xi'an Huanuo Environmental Protection Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xi'an Huanuo Environmental Protection Co Ltd filed Critical Xi'an Huanuo Environmental Protection Co Ltd
Priority to CN201710248049.2A priority Critical patent/CN106957800A/en
Publication of CN106957800A publication Critical patent/CN106957800A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor

Abstract

The present invention relates to a kind of cultural method of COD degradation bacterium, comprise the following steps:(1) nutrient agar is prepared, (2) activate COD degradation bacterium in nutrient broth medium, institute (3) takes the COD degradation bacterium solution diluted to distinguish 200 microlitres with liquid-transfering gun.It is an advantage of the invention that:The COD degradation bacterium cultivated by the present invention, can effectively improve COD degradation rate, not pollute the environment.

Description

The cultural method of COD degradation bacterium
Technical field
The present invention relates to a kind of cultural method of COD degradation bacterium.
Background technology
At aspect of curbing environmental pollution, it will usually using the reduction COD processing of COD degradation bacterium, the drawbacks of its is existing It is:Effect is undesirable, effective removal how is cleaned, still in conceptual phase.Pertinent literature is not found through retrieval.
The content of the invention
To overcome the defect of prior art, the present invention provides a kind of cultural method of COD degradation bacterium, technical side of the invention Case is:
A kind of cultural method of COD degradation bacterium, comprises the following steps:
(1) nutrient agar is prepared, specific preparation method is as follows:Weigh 10 parts of peptone, 5 parts of beef extract powder, fine jade 18 parts of cosmetics, 5 parts of sodium chloride is mixed according to proportioning, is heated after mixing in micro-wave oven, the heat time:1-2min, heating Temperature:70-80 DEG C, untill agar powder melts completely, it is put into high-pressure sterilizing pot and is sterilized, 121 DEG C of sterilising temp, time 30min, is then poured into sterilized desiccation culture ware, each culture dish pours into 15ml in Biohazard Safety Equipment, will be trained well The culture dish for supporting base is placed in safety cabinet to cooling, cool time:4-5h, chilling temperature:25 DEG C of room temperature, can be put into ice after cooling Case refrigeration is standby;
(2) by the activation of COD degradation bacterium in nutrient broth medium, the preparation method of the nutrient broth medium is such as Under:10 parts of peptone is weighed, then 3 parts of beef extract powder, 5 parts of sodium chloride mix, 30 DEG C of constant incubator training is put into after mixing Support 2 days, flat board coating then carried out to the COD degradation bacterium for having activated and having grown in broth bouillon in superclean bench, COD degradation bacterium solution concentration is subjected to gradient dilution simultaneously, 10-2 powers, 10-4 powers ,-the 6 of 10 times are diluted to respectively Side and 10-8 powers.
(3) take the COD degradation bacterium solution diluted to distinguish 200 microlitres with liquid-transfering gun, be injected separately into the battalion in culture dish Support on agar medium, it is with spreading rod that COD degradation bacterium solution coating is uniform, and enter rower in the bottom of culture dish with marking pen In note, the constant incubator for being then placed in 35 DEG C, cultivated, after 1-2 days, COD degradation bacterium on observation nutrient agar Growing state, mark the COD degradation bacterium of the different shape grown on culture medium, and reference number respectively with marking pen, then Plate streaking is carried out to the COD degradation bacterium of mark;
(4) flat board pulled is put into constant incubator, 35 DEG C, cultivated 1-2 days, the life of COD degradation bacterium on observation flat board It is long, and dyeing microscopic examination is carried out to the COD degradation bacterium grown, its morphological feature is recorded, the operation is repeated, until being seen under microscope Untill the COD degradation bacterium form Economical Purification examined, the COD degradation bacterium of purifying is carried out to carry out inclined-plane in test tube slant culture medium Line preservation.
Specific dilution process is in described step (2):10ml distilled water is taken to be put into test tube with syringe, by distilled water Sterilized, in Biohazard Safety Equipment, 100 microlitres of strain solution taken with liquid-transfering gun, is injected into the test tube of sterile distilled water, Now, the strain solution concentration in the test tube is 10-2 powers, and strain solution is diluted to by same method respectively 10 for-4 times Side, 10-6 powers and-the 8 of 10 powers.
Described step (3) plate streaking method is as follows:In Biohazard Safety Equipment, alcolhol burner is lighted, with the top of oese The COD degradation bacterium of mark sequence number is touched, is drawn on nutrient agar, the COD degradation bacterium of each sequence number draws four on culture medium Oese, is placed on alcolhol burner and burns, repeat by area per standardized area.
The preparation method of described step (4) test tube slant culture medium:Weigh 10 parts of peptone, 5 parts of beef extract powder, agar 18 parts of powder, 5 parts of sodium chloride is mixed according to proportioning, is heated after mixing in micro-wave oven, the heat time:1-2min, heating temperature Degree:70-80 DEG C, untill agar powder melts completely, taken with 10ml syringe in 5ml injecting tubes, test tube is put into 2L's Beaker is wrapped up, and is then placed in high-pressure sterilizing pot and is sterilized, and is sterilized 121 DEG C, after sterilizing, test tube is taken out by time 30min Inclined-plane is put, the standard of putting on inclined-plane is the 2/3 of of length no more than whole test tube length of nutrient agar on inclined-plane, stood 8h, it is to be condensed after, be put into refrigerator cold-storage.
It is an advantage of the invention that:The COD degradation bacterium cultivated by the present invention, can effectively improve COD degradation rate, no Pollute the environment.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and It is apparent.But these embodiments are only exemplary, do not constitute any limitation to the scope of the present invention.People in the art Member to the details and form of technical solution of the present invention it should be understood that can enter without departing from the spirit and scope of the invention Row modification is replaced, but these modifications and replacement are each fallen within protection scope of the present invention.
The present invention relates to a kind of cultural method of COD degradation bacterium, comprise the following steps:
(1) nutrient agar is prepared, specific preparation method is as follows:Peptone 10g/L, beef extract powder 5g/L are weighed, Agar powder 18g/L, sodium chloride 5g/L, are mixed according to proportioning, are heated after mixing in micro-wave oven, the heat time:1-2min, Heating-up temperature:70-80 DEG C, untill agar powder melts completely, it is put into high-pressure sterilizing pot and is sterilized, 121 DEG C of sterilising temp, when Between 30min, then poured into Biohazard Safety Equipment in sterilized desiccation culture ware, each culture dish pours into 15ml, will be good The culture dish of culture medium is placed in safety cabinet to cooling, cool time:4-5h, chilling temperature:25 DEG C of room temperature, can be put into after cooling Refrigerator cold-storage is standby;
(2) by the activation of COD degradation bacterium in nutrient broth medium, the preparation method of the nutrient broth medium is such as Under:10 parts of peptone is weighed, then 3 parts of beef extract powder, 5 parts of sodium chloride mix, 30 DEG C of constant incubator training is put into after mixing Support 2 days, flat board coating then carried out to the COD degradation bacterium for having activated and having grown in broth bouillon in superclean bench, COD degradation bacterium solution concentration is subjected to gradient dilution simultaneously, 10-2 powers, 10-4 powers ,-the 6 of 10 times are diluted to respectively Side and 10-8 powers.
(3) take the COD degradation bacterium solution diluted to distinguish 200 microlitres with liquid-transfering gun, be injected separately into nutrient agar culture It is with spreading rod that COD degradation bacterium solution coating is uniform on base, and (mark temperature is labeled with marking pen in the bottom of culture dish Degree, date etc.), in the constant incubator for being then placed in 35 DEG C, cultivated, after 1-2 days, COD degradation bacterium on observation culture medium Growing state, mark the COD degradation bacterium of the different shape grown on culture medium, and reference number respectively with marking pen, then Plate streaking is carried out to the COD degradation bacterium of mark;
(4) flat board pulled is put into constant incubator, 35 DEG C, cultivated 1-2 days, the life of COD degradation bacterium on observation flat board It is long, and dyeing microscopic examination is carried out to the COD degradation bacterium grown, its morphological feature is recorded, the operation is repeated, until being seen under microscope Untill the COD degradation bacterium form Economical Purification examined, the COD degradation bacterium of purifying is carried out to carry out inclined-plane in test tube slant culture medium Line preservation.
Specific dilution process is in described step (2):10ml distilled water is taken to be put into test tube with syringe, by distilled water Sterilized, in Biohazard Safety Equipment, 100 microlitres of strain solution taken with liquid-transfering gun, is injected into the test tube of sterile distilled water, Now, the strain solution concentration in the test tube is 10-2 powers, and strain solution is diluted to by same method respectively 10 for-4 times Side, 10-6 powers and-the 8 of 10 powers.
Described step (3) plate streaking method is as follows:In Biohazard Safety Equipment, alcolhol burner is lighted, with the top of oese The COD degradation bacterium of mark sequence number is touched, is drawn on nutrient agar, the COD degradation bacterium of each sequence number draws four on culture medium Oese, is placed on alcolhol burner and burns, repeat by area per standardized area.
The preparation method of described step (4) test tube slant culture medium:Weigh 10 parts of peptone, 5 parts of beef extract powder, agar 18 parts of powder, 5 parts of sodium chloride is mixed according to proportioning, is heated after mixing in micro-wave oven, the heat time:1-2min, heating temperature Degree:70-80 DEG C, untill agar powder melts completely, taken with 10ml syringe in 5ml injecting tubes, test tube is put into 2L's Beaker is wrapped up, and is then placed in high-pressure sterilizing pot and is sterilized, and is sterilized 121 DEG C, after sterilizing, test tube is taken out by time 30min Inclined-plane is put, the standard of putting on inclined-plane is the 2/3 of of length no more than whole test tube length of nutrient agar on inclined-plane, stood 8h, it is to be condensed after, be put into refrigerator cold-storage.
Embodiment:Activated COD degradation bacterium is added in liquid medium within, wherein COD degradation bacterium is trained with liquid The weight ratio for supporting base is 1: 10, in 30 DEG C of constant incubator, after culture 24h, determines the content of COD in culture medium, blank sample In, COD content is 0.283g/L, is added after the COD degradation bacterium, and COD content is 0.086g/L.

Claims (4)

1. a kind of cultural method of COD degradation bacterium, it is characterised in that comprise the following steps:
(1) nutrient agar is prepared, specific preparation method is as follows:Weigh 10 parts of peptone, 5 parts of beef extract powder, agar powder 18 parts, 5 parts of sodium chloride is mixed according to proportioning, is heated after mixing in micro-wave oven, the heat time:1-2min, heating temperature Degree:70-80 DEG C, untill agar powder melts completely, it is put into high-pressure sterilizing pot and is sterilized, 121 DEG C of sterilising temp, time 30min, is then poured into sterilized desiccation culture ware, each culture dish pours into 15ml in Biohazard Safety Equipment, will be trained well The culture dish for supporting base is placed in safety cabinet to cooling, cool time:4-5h, chilling temperature:25 DEG C of room temperature, can be put into ice after cooling Case refrigeration is standby;
(2) by the activation of COD degradation bacterium in nutrient broth medium, the preparation method of the nutrient broth medium is as follows:Claim 10 parts of peptone is taken, then 3 parts of beef extract powder, 5 parts of sodium chloride mixed, and 30 DEG C of constant incubator culture 2 is put into after mixing My god, flat board coating then is carried out to the COD degradation bacterium for having activated and having grown in broth bouillon in superclean bench, simultaneously COD degradation bacterium solution concentration is subjected to gradient dilution, 10-2 powers are diluted to respectively, 10-4 powers ,-the 6 of 10 powers with And 10-8 powers.
(3) take the COD degradation bacterium solution diluted to distinguish 200 microlitres with liquid-transfering gun, be injected separately into the nutrition fine jade in culture dish It is with spreading rod that COD degradation bacterium solution coating is uniform on fat culture medium, and be labeled with marking pen in the bottom of culture dish, In the constant incubator for being then placed in 35 DEG C, cultivated, after 1-2 days, the life of COD degradation bacterium on observation nutrient agar Long situation, the COD degradation bacterium of the different shape grown on culture medium, and reference number are marked with marking pen respectively, then to mark The COD degradation bacterium of note carries out plate streaking;
(4) flat board pulled is put into constant incubator, 35 DEG C, cultivated 1-2 days, the growth of COD degradation bacterium on observation flat board, and Dyeing microscopic examination is carried out to the COD degradation bacterium grown, its morphological feature is recorded, the operation is repeated, until micro- Microscopic observation Untill COD degradation bacterium form Economical Purification, the COD degradation bacterium of purifying is carried out to carry out inclined-plane line in test tube slant culture medium Preservation.
2. the cultural method of COD degradation bacterium according to claim 1, it is characterised in that specific dilute in described step (2) The method of releasing is:Take 10ml distilled water to be put into test tube with syringe, distilled water is sterilized, in Biohazard Safety Equipment, with shifting Liquid rifle takes 100 microlitres of strain solution, is injected into the test tube of sterile distilled water, now, the strain solution concentration in the test tube For 10-2 powers, same method, strain solution is diluted to respectively 10-4 powers ,-the 6 of 10 powers and-the 8 of 10 times Side.
3. the cultural method of COD degradation bacterium according to claim 1, it is characterised in that described step (3) plate streaking Method is as follows:In Biohazard Safety Equipment, alcolhol burner is lighted, with the COD degradation bacterium that mark sequence number is touched at the top of oese, is drawn in nutrition On agar medium, oese is placed on alcolhol burner by the COD degradation bacterium of each sequence number in the areas of culture medium Shang Hua tetra- per standardized area Burning, is repeated.
4. the cultural method of COD degradation bacterium according to claim 1, it is characterised in that described step (4) test tube slant The preparation method of culture medium:10 parts of peptone is weighed, 5 parts of beef extract powder, 18 parts of agar powder, 5 parts of sodium chloride is carried out according to proportioning Mixing, is heated, the heat time after mixing in micro-wave oven:1-2min, heating-up temperature:70-80 DEG C, melt completely to agar powder and be Only, taken with 10ml syringe in 5ml injecting tubes, the beaker that test tube is put into 2L is wrapped up, and is then placed in autoclaving Pot is sterilized, and is sterilized 121 DEG C, time 30min, after sterilizing, test tube is taken out into pendulum inclined-plane, the standard of putting on inclined-plane is on inclined-plane The 2/3 of of length no more than whole test tube length of nutrient agar, stand 8h, it is to be condensed after, be put into refrigerator cold-storage.
CN201710248049.2A 2017-04-17 2017-04-17 The cultural method of COD degradation bacterium Pending CN106957800A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710248049.2A CN106957800A (en) 2017-04-17 2017-04-17 The cultural method of COD degradation bacterium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710248049.2A CN106957800A (en) 2017-04-17 2017-04-17 The cultural method of COD degradation bacterium

Publications (1)

Publication Number Publication Date
CN106957800A true CN106957800A (en) 2017-07-18

Family

ID=59483828

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710248049.2A Pending CN106957800A (en) 2017-04-17 2017-04-17 The cultural method of COD degradation bacterium

Country Status (1)

Country Link
CN (1) CN106957800A (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001062494A (en) * 1999-08-26 2001-03-13 Takaaki Arai Treatment of waste paper sludge
CN102747015A (en) * 2012-06-21 2012-10-24 北京理工大学 Denitrification acinetobacters and use thereof
CN104403968A (en) * 2014-09-28 2015-03-11 南京工业大学 Bacillius firmus GY-49, screening method and applications thereof
CN105132323A (en) * 2015-09-08 2015-12-09 常州大学 Salt-tolerance bacillus and application thereof in high-salinity wastewater treatment

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001062494A (en) * 1999-08-26 2001-03-13 Takaaki Arai Treatment of waste paper sludge
CN102747015A (en) * 2012-06-21 2012-10-24 北京理工大学 Denitrification acinetobacters and use thereof
CN104403968A (en) * 2014-09-28 2015-03-11 南京工业大学 Bacillius firmus GY-49, screening method and applications thereof
CN105132323A (en) * 2015-09-08 2015-12-09 常州大学 Salt-tolerance bacillus and application thereof in high-salinity wastewater treatment

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
周德庆 主编: "《微生物学实验手册》", 31 December 1986 *

Similar Documents

Publication Publication Date Title
CN105441375B (en) Induction phytophthora blight of pepper generates sporangium and discharges the method for zoospore
CN103710434B (en) A kind of making method of marrow chromosome G band
CN105039220A (en) Bacillus methylotrophicus AR3, bacillus subtilis AR4 and bacillus amyloliquefaciens AR10 and application thereof
CN106906275A (en) Microbial limit tests in a kind of clearing and activating the channels and collaterals capsule
CN107338205A (en) A kind of Bacillus cereus strain EAb 3 and its application
CN106957800A (en) The cultural method of COD degradation bacterium
CN105838683A (en) Method for proliferation of mink canine distemper virus by applying novel cell microcarrier
CN104403968B (en) A kind of bacillus firmus GY 49 and its screening technique and application
CN107058105A (en) The cultural method of nitrite degradation bacterium
CN107686813A (en) A kind of Euglena high-density cultivation method
CN106939285A (en) The cultural method of deodorization bacterium
CN104232564A (en) Culture medium suitable for preparing hog cholera vaccines from ST cell and using method thereof
CN106947695A (en) The cultural method of ammonia nitrogen degradation bacterium
CN106978344A (en) The cultural method of Phenol-degrading Bacteria Strains
CN104132940A (en) Convenient observation method of orchid mycorrhiza microstructure
CN107058104A (en) The cultural method of oil degradation bacteria
CN104726339B (en) A kind of immobilization cultural method of microalgae
CN105875406B (en) A kind of induction of Borneolum tree callus and enrichment procedure
CN110055180A (en) A kind of culture collection process of anaerobic bacteria
CN106978345A (en) The cultural method of biological demulsifying agent producing strains
CN103710435B (en) marrow chromosome extraction kit
CN104403986A (en) Microbe spore culture method and equipment
CN105543098B (en) A kind of culture medium and its application for batrachos-permum
CN103146597A (en) Method for preparing photosynthetic bacteria liquid
CN107091920A (en) A kind of inspection method of the micro- middle biological limit of loins-strengthening and kidney-invigorating bolus

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170718

RJ01 Rejection of invention patent application after publication