CN106938958A - A kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer - Google Patents
A kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer Download PDFInfo
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- CN106938958A CN106938958A CN201710293388.2A CN201710293388A CN106938958A CN 106938958 A CN106938958 A CN 106938958A CN 201710293388 A CN201710293388 A CN 201710293388A CN 106938958 A CN106938958 A CN 106938958A
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- Prior art keywords
- dipel
- organic fertilizer
- kitchen garbage
- bacillus
- preparation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer, belong to organic fertilizer production field, key step includes：The aerobic degradation and two steps of inoculation of Dipel of kitchen garbage and husk mixture.On the one hand this method remains the high efficiency that high-efficiency aerobic degradation technique is handled kitchen garbage, on the other hand the content of Dipel in organic fertilizer is improved, both the feature with organic fertilizer also contains a large amount of Dipels that there are prevention and control to act on to plant pest to the organic fertilizer produced.
Description
Technical field
The present invention relates to organic fertilizer production field, particularly a kind of load high concentration Dipel kitchen garbage organic fertilizer
Preparation method.
Background technology
Kitchen garbage is production and the common biomass solid wastes produced in life, wherein including substantial amounts of easy drop
Solve organic matter, these organic emissions into environment after can in the presence of various microorganisms rapid corruption, produce substantial amounts of oozes
Filtrate, corrupt gas can also grow pathogen simultaneously, and the production and living to environment and people cause to have a strong impact on.
Aerobic degradation technology is to utilize thermophilic microorganism under the high temperature conditions（50~60 ℃）Kitchen garbage is converted rapidly
For the resource technology of organic fertilizer, this method has transformation efficiency high（Kitchen garbage is converted into organic fertilizer in 96 hours）,
The characteristics of product content of organic matter is high, but the environmental condition required for aerobic degradation technology is 50 ~ 60 DEG C, this temperature strip
Part can allow a large amount of thermophilic normal temperature microorganisms to be in dormancy or dead state, wherein comprising microorganism largely beneficial to plant growth,
Substantial amounts of thermophilic microorganism is included in the organic fertilizer produced under these conditions, after organic fertilizer is delivered into farmland, by
Changed in temperature environment, thermophilic microorganism is in inactivated state.Therefore, contain in the organic fertilizer produced by this way
The thermophilic normal temperature micro organism quantity having is extremely limited, and the microorganism beneficial to plant is also extremely limited.
Dipel is common bacterium in a kind of nature, and this microorganism can discharge one during sporulation
The albumen to insect with killing action is planted, therefore is widely used in the prevention and control of plant diseases, pest control of crops.Because Dipel is suitable
Grown preferably under normal temperature condition, therefore in the aerobic degradation under the conditions of 50 ~ 60 DEG C, Dipel can not be good
Growth and breeding, also constrain the number of viable of Dipel in product.
The content of the invention
The purpose of the present invention be for it is above-mentioned exist problem there is provided one kind load high concentration Dipel kitchen garbage have
The preparation method of machine fertilizer, the organic fertilizer that profit is produced in this way can not only realize that kitchen garbage turns to the quick of organic fertilizer
Change, while the organic fertilizer produced is also containing a large amount of Dipels that there are prevention and control to act on to plant pest.
Technical scheme：
A kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer, comprises the following steps：
（1）Stalk is dried and 1 ~ 10mm is crushed to；Kitchen garbage draining that dining room is fetched, drain and dry as far as possible；
（2）By microorganism Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans
Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009,
Seven kinds of Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 are micro-
Bacterization is cultivated 24 hours in 500ml LB culture mediums under the conditions of 55 DEG C.Obtained microbial inoculum is mixed micro-
Bacteria agent A.The formula of wherein every liter LB culture medium is：Peptone 10g, dusty yeast 5g, sodium chloride 5g, pH value 7.4；
（3）By stalk and kitchen garbage according to 34:66 mass ratio mixing；Mixture is placed in model TBH-CSCL-40's
In good time moisturizing in aerobic degradation, aerobic degradation is carried out in kitchen garbage biochemical reactor, material water ratio is maintained between 55%
~ 60%, while adding microbial bacterial agent A into reactor.It is 20rpm that rotating speed of agitator is set in course of reaction, and temperature conditionss are
55 DEG C, material water ratio is between 55% ~ 60% in appropriate moisturizing maintenance reaction device；
（4）Dipel is inoculated in 20 liters of LB fluid nutrient mediums, is placed in after being cultivated 2 days under normal temperature and obtains Dipel
Bacterium solution；
（5）After aerobic degradation terminates, cancel system heating, by 20 L Dipel bacterium solutions after material is cooled to normal temperature
All it is added in material, the temperature remains within the normal range persistently stirs and divulge information for reactor, until material is spontaneously dried, that is, obtains containing largely
The organic fertilizer of Dipel viable bacteria.
The advantages of the present invention are：
（1）Advantage of the aerobic degradation technology to kitchen garbage rapid conversion is maintained, aerobic degradation hot environment is compensate for
Killing and inhibitory action to thermophilic normal temperature microorganism；
（2）Obtained organic fertilizer product had both had abundant organic fertilizer nutrition species, nutritive element content height, had not destroyed soil texture
The features such as, also containing a large amount of Dipel viable bacterias, this microorganism can provide nutrition and various growths for the growth of plant
The factor, prevents the generation of plant disease.
Embodiment
The present invention is described in further detail by following examples, but the technology contents that are described of the present embodiment be it is illustrative,
Rather than it is limited, protection scope of the present invention should not be limited to according to this.
Embodiment：
A kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer, comprises the following steps：
（1）Stalk is dried and 1 ~ 10mm is crushed to；Kitchen garbage draining that dining room is fetched, drain and dry as far as possible；
（2）By microorganism Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans
Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009,
Seven kinds of Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 are micro-
Bacterization is cultivated 24 hours in 500ml LB culture mediums under the conditions of 55 DEG C.Obtained microbial inoculum is mixed micro-
Bacteria agent A.The formula of wherein every liter LB culture medium is：Peptone 10g, dusty yeast 5g, sodium chloride 5g, pH value 7.4；
（3）By stalk and kitchen garbage according to 34:66 mass ratio mixing；Mixture is placed in model TBH-CSCL-40's
In good time moisturizing in aerobic degradation, aerobic degradation is carried out in kitchen garbage biochemical reactor, material water ratio is maintained between 55%
~ 60%, while adding microbial bacterial agent A into reactor.It is 20rpm that rotating speed of agitator is set in course of reaction, and temperature conditionss are
55 DEG C, material water ratio is between 55% ~ 60% in appropriate moisturizing maintenance reaction device；
（4）Dipel is inoculated in 20 liters of LB fluid nutrient mediums, is placed in after being cultivated 2 days under normal temperature and obtains Dipel
Bacterium solution；
（5）After aerobic degradation terminates, cancel system heating, by 20 L Dipel bacterium solutions after material is cooled to normal temperature
All it is added in material, the temperature remains within the normal range persistently stirs and divulge information for reactor, until material is spontaneously dried, that is, obtains containing largely
The organic fertilizer of Dipel viable bacteria.
Reaction is sampled after terminating from material, and the method according to standard NY525-2012 is carried out to organic fertilizer sample
Detection, test result indicate that every Testing index reaches or better than the requirement of defined in standard, wherein, TOC
For 90%, total nutrient content is 7.8%, and moisture content 24%, pH value is 7.1.Method according to standard NY/T 227-1994
Viable count in organic fertilizer sample is detected, testing result shows that the viable count in sample reaches 1.6 hundred million/g.
Claims (4)
1. a kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer, comprises the following steps：
（1）Stalk is dried and 1 ~ 10mm is crushed to；Kitchen garbage draining that dining room is fetched, drain and dry as far as possible；
（2）By microorganism Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans
Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009,
Seven kinds of Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 are micro-
Bacterization is cultivated 24 hours in 500ml LB culture mediums under the conditions of 55 DEG C, obtained microbial inoculum is mixed micro-
Bacteria agent A, wherein the formula of every liter of LB culture medium is：Peptone 10g, dusty yeast 5g, sodium chloride 5g, pH value 7.4；
（3）By stalk and kitchen garbage according to 34:66 mass ratio mixing；Mixture is placed in model TBH-CSCL-40's
In good time moisturizing in aerobic degradation, aerobic degradation is carried out in kitchen garbage biochemical reactor, material water ratio is maintained between 55%
~ 60%, set rotating speed of agitator for 20rpm while being added into reactor in microbial bacterial agent A, course of reaction, temperature conditionss are
55 DEG C, material water ratio is between 55% ~ 60% in appropriate moisturizing maintenance reaction device；
（4）Dipel is inoculated in 20 liters of LB fluid nutrient mediums, is placed in after being cultivated 2 days under normal temperature and obtains Dipel
Bacterium solution；
（5）After aerobic degradation terminates, cancel system heating, by 20 L Dipel bacterium solutions after material is cooled to normal temperature
All it is added in material, the temperature remains within the normal range persistently stirs and divulge information for reactor, until material is spontaneously dried, that is, obtains containing largely
The organic fertilizer of Dipel viable bacteria.
2. a kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer according to claim 1, it is special
Levy and be：The manufacturing process of organic fertilizer uses aerobic thermophilic composting technology, and actual conditions is：Temperature is between 50 ~ 60 DEG C, moisture content
Between 55% ~ 65%, agitating paddle and blower fan keep continuous running.
3. a kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer according to claim 1, it is special
Levy and be：Microbial bacterial agent A is by Bacillus sp. MN-07, Bacillus coagulans, Bacillus coagulans
Strain KM-1, Bacillus coagulans 36D1, Bacillus licheniformis strain Pb-WC09009,
Seven kinds of Bacillus tequilensis strain CGX5-1, Bacillus licheniformis strain TS-11 are micro-
Biotic component.
4. a kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer according to claim 1, it is special
Levy and be：The microorganism of cooling stage inoculation is Dipel zymocyte liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710293388.2A CN106938958A (en) | 2017-04-28 | 2017-04-28 | A kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer |
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| CN201710293388.2A CN106938958A (en) | 2017-04-28 | 2017-04-28 | A kind of preparation method for loading high concentration Dipel kitchen garbage organic fertilizer |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529575A (en) * | 2015-01-16 | 2015-04-22 | 南开大学 | Method for producing high-efficiency organic fertilizer from kitchen waste |
| CN104672028A (en) * | 2015-01-05 | 2015-06-03 | 大连理工大学 | Insect-resistant bio-organic fertilizer and preparation method thereof |
| CN105176881A (en) * | 2015-10-16 | 2015-12-23 | 杭州标航生物科技有限公司 | High-efficiency engineering bacteria agent and method for producing active biological organic fertilizer |
| CN106588416A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Preparation method of special insect-resistant bio-organic fertilizer for blueberries |
-
2017
- 2017-04-28 CN CN201710293388.2A patent/CN106938958A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104672028A (en) * | 2015-01-05 | 2015-06-03 | 大连理工大学 | Insect-resistant bio-organic fertilizer and preparation method thereof |
| CN104529575A (en) * | 2015-01-16 | 2015-04-22 | 南开大学 | Method for producing high-efficiency organic fertilizer from kitchen waste |
| CN105176881A (en) * | 2015-10-16 | 2015-12-23 | 杭州标航生物科技有限公司 | High-efficiency engineering bacteria agent and method for producing active biological organic fertilizer |
| CN106588416A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Preparation method of special insect-resistant bio-organic fertilizer for blueberries |
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Application publication date: 20170711 |