CN106858234A - A kind of honeysuckle compound beverage and preparation method thereof - Google Patents
A kind of honeysuckle compound beverage and preparation method thereof Download PDFInfo
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- CN106858234A CN106858234A CN201611234272.3A CN201611234272A CN106858234A CN 106858234 A CN106858234 A CN 106858234A CN 201611234272 A CN201611234272 A CN 201611234272A CN 106858234 A CN106858234 A CN 106858234A
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- honeysuckle
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- leaf
- distillate
- ethanol water
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- 241000205585 Aquilegia canadensis Species 0.000 title claims abstract description 149
- 235000013361 beverage Nutrition 0.000 title claims abstract description 41
- 150000001875 compounds Chemical class 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 30
- 229930003935 flavonoid Natural products 0.000 claims abstract description 28
- 150000002215 flavonoids Chemical class 0.000 claims abstract description 28
- 235000017173 flavonoids Nutrition 0.000 claims abstract description 28
- 239000000843 powder Substances 0.000 claims abstract description 28
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 claims abstract description 27
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 claims abstract description 27
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 claims abstract description 27
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 claims abstract description 27
- 229940074393 chlorogenic acid Drugs 0.000 claims abstract description 27
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 claims abstract description 27
- 235000001368 chlorogenic acid Nutrition 0.000 claims abstract description 27
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 claims abstract description 27
- 239000000284 extract Substances 0.000 claims abstract description 27
- 239000000287 crude extract Substances 0.000 claims abstract description 25
- 235000009508 confectionery Nutrition 0.000 claims abstract description 24
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 18
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 15
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 15
- 239000011718 vitamin C Substances 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 37
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 37
- 239000000706 filtrate Substances 0.000 claims description 19
- 201000002282 venous insufficiency Diseases 0.000 claims description 14
- 238000004821 distillation Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 10
- 239000011347 resin Substances 0.000 claims description 9
- 229920005989 resin Polymers 0.000 claims description 9
- 239000002131 composite material Substances 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- 229910052737 gold Inorganic materials 0.000 claims description 3
- 239000010931 gold Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 229910052709 silver Inorganic materials 0.000 claims description 3
- 239000004332 silver Substances 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims description 2
- 230000003115 biocidal effect Effects 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 description 14
- 239000003480 eluent Substances 0.000 description 7
- 238000005374 membrane filtration Methods 0.000 description 7
- 238000001556 precipitation Methods 0.000 description 7
- 238000001816 cooling Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000001408 fungistatic effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 206010010144 Completed suicide Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 229910021502 aluminium hydroxide Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- -1 bifidus factor Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicinal Preparation (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The present invention relates to a kind of honeysuckle compound beverage and preparation method thereof, belong to honeysuckle drink technical field.The honeysuckle compound beverage that the present invention is provided includes the component of following weight/mass percentage composition：Honeysuckle distillate 60~80%, honeysuckle crude flavonoid powder medicinal extract 5~15%, honeysuckle Thick many candies 1~15%, honeysuckle-leaf chlorogenic acid crude extract 1~15%, bifidus factor 1~5%, vitamin C 0.02~0.05%, white granulated sugar 5~20%, balance of water.The honeysuckle compound beverage biocidal efficacies that the present invention is prepared are strong, can realize that the comprehensive high-efficiency of honeysuckle resource is utilized.
Description
Technical field
The present invention relates to honeysuckle drink technical field, more particularly to a kind of honeysuckle compound beverage and preparation method thereof.
Background technology
Honeysuckle medicinal history is long, is the important Chinese medicine material of China's dietotherapeutic,《Compendium of Materia Medica》It has been discussed in detail
Effect with " long term usage is made light of one's life by commiting suicide, and is promoted longevity ", and《Mingyi Bielu》In be listed in top grade.
Prepare drink, including the distilled liquid of honeysuckle, honeysuckle cold tea, honeysuckle hypoglycemic using honeysuckle mostly in the market
Tea, Honeysuckle beer, honeysuckle ecological water etc., while also there is the beverage that development is combined with several kinds of Chinese medicinal materials.But honeysuckle-leaf one
Directly it is considered as non-medicinal effects and largely discards, prior art also has certain shortcoming in terms of the comprehensive utilization of honeysuckle.
The content of the invention
It is an object of the invention to provide a kind of honeysuckle compound beverage and preparation method thereof.The honeysuckle that the present invention is provided
Composite beverage biocidal efficacies are strong, can realize that the comprehensive high-efficiency of honeysuckle resource is utilized.
The invention provides a kind of honeysuckle compound beverage, including following weight/mass percentage composition component：
Honeysuckle distillate 60~80%, honeysuckle crude flavonoid powder medicinal extract 5~15%, honeysuckle Thick many candies 1~15%, gold and silver
Floral leaf chlorogenic acid crude extract 1~15%, bifidus factor 1~5%, vitamin C 0.02~0.05%, white granulated sugar 5~20% is remaining
It is water to measure.
Preferably, the preparation method bag of the honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract and honeysuckle Thick many candies
Include following steps：
1) it is 1kg according to mass volume ratio by honeysuckle and water：(5~30) L mixes, air-distillation, obtains honeysuckle steaming
Distillate and vinasse；
2) vinasse is precipitated using ethanol water, is filtrated to get alcohol hypostasis and filtrate, the alcohol hypostasis is
Honeysuckle Thick many candies；
3) by the step 2) filtrate that obtains adsorbed using macroreticular resin, eluted using ethanol water,
Honeysuckle crude flavonoid powder medicinal extract is obtained after eluent is concentrated.
Preferably, the step 1) distillation time be no less than 1h.
Preferably, the step 3) ethanol water in ethanol volume fraction be 30~50%, elution flow rate is 3
~5BV/h, ethanol water consumption is 4~6 times of column volumes.
Preferably, the preparation method of the honeysuckle-leaf chlorogenic acid crude extract is comprised the following steps：
Honeysuckle-leaf is leached into 5~15h in ethanol water, regulation pH value is 2~6, with 10~40 times of honeysuckle-leaf
The boiling water mixing of quality, stirs 5~60min, obtains honeysuckle chlorogenic acid crude extract.
Preferably, the quality of the honeysuckle-leaf and the volume ratio of ethanol water are 1kg：(1~6) L.
Preferably, the volume fraction of ethanol is 20~90% in the ethanol water.
Present invention also offers the preparation method of honeysuckle compound beverage described in above-mentioned technical proposal, comprise the following steps：
The honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract, honeysuckle Thick many candies, honeysuckle-leaf chlorogenic acid are slightly carried
The mixing of thing, bifidus factor, vitamin C, white granulated sugar and water, stands and is no less than 24h, is filtrated to get honeysuckle compound beverage.
Preferably, the aperture of the filtering filter membrane is 0.2~0.45 μm.
The invention provides a kind of honeysuckle compound beverage.The present invention with honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract,
Honeysuckle Thick many candies and honeysuckle-leaf chlorogenic acid crude extract, bifidus factor, vitamin C, white granulated sugar and water compounding, obtain honeysuckle
Composite beverage.The honeysuckle compound beverage that the present invention is provided is a kind of honeysuckle flower product of neomorph, and biocidal efficacies are strong, Neng Goushi
The comprehensive high-efficiency of cash honeysuckle flower resource is utilized.
Specific embodiment
The invention provides a kind of honeysuckle compound beverage, including following weight/mass percentage composition component：
Honeysuckle distillate 60~80%, honeysuckle crude flavonoid powder medicinal extract 5~15%, honeysuckle Thick many candies 1~15%, gold and silver
Floral leaf chlorogenic acid crude extract 1~15%, bifidus factor 1~5%, vitamin C 0.02~0.05%, white granulated sugar 5~20% is remaining
It is water to measure.
The present invention originates without special restriction to the honeysuckle, using honeysuckle well known to those skilled in the art
Commercially available prod.
The honeysuckle compound beverage that the present invention is provided includes the honeysuckle distillate that weight/mass percentage composition is 60~80%, excellent
Elect 65~75% as.
The honeysuckle compound beverage that the present invention is provided includes the honeysuckle Thick many candies that weight/mass percentage composition is 5~15%, excellent
Elect 10~12% as.
The honeysuckle compound beverage that the present invention is provided soaks including the honeysuckle crude flavonoid powder that weight/mass percentage composition is 1~15%
Cream, preferably 2~8%.
In the present invention, the preparation method of the honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract and honeysuckle Thick many candies
Comprise the following steps：
1) it is 1kg according to mass volume ratio by honeysuckle and water：(5~30) L mixes, air-distillation, obtains honeysuckle steaming
Distillate and vinasse；
2) vinasse is precipitated using ethanol water, is filtrated to get alcohol hypostasis and filtrate, the alcohol hypostasis is
Honeysuckle Thick many candies；
3) by the step 2) filtrate that is filtrated to get adsorbed using macroreticular resin, washed using ethanol water
It is de-, honeysuckle crude flavonoid powder medicinal extract is obtained after eluent is concentrated.
Honeysuckle and water are 1kg according to mass volume ratio by the present invention：(5~30) L mixing is distilled, air-distillation
DEG C, obtain honeysuckle distillate and vinasse.In the present invention, the quality of the honeysuckle and the volume ratio of water are preferably
1kg：(10~25) L, more preferably 1kg：(15~20) L；The time of the distillation is preferably no less than 1h, more preferably no less than
5h.In the present invention, the steam cooling of the distillation obtains distillate.
After obtaining honeysuckle distillate and vinasse, the present invention is heavy to honeysuckle vinasse using ethanol water
Form sediment, be filtrated to get alcohol hypostasis and filtrate, the alcohol hypostasis is honeysuckle Thick many candies.In the present invention, the ethanol water
Volume fraction is preferably 90~96%, more preferably 95%, and after ethanol water mixes with honeysuckle distillate raffinate, ethanol exists
Volume fraction in mixed solution is preferably 85%；The number of times of the precipitation is preferably 2~3 times.The present invention is to the ethanol
Originate without special restriction, using commercially available edible rank ethanol well known to those skilled in the art.
After the filtering, the present invention is adsorbed the filtrate being filtrated to get using macroreticular resin, using ethanol water
Solution is eluted, and honeysuckle crude flavonoid powder medicinal extract is obtained after eluent is concentrated.The present invention is not special to the macroreticular resin
Restriction, using macroreticular resin well known to those skilled in the art, such as D101 or HPD-450.In the present invention, the second
The volume fraction of ethanol is 30~50%, more preferably 35~45%, most preferably 40% in alcohol solution；The elution process
The flow velocity of middle ethanol water is preferably 3~5BV/h, more preferably 4BV/h；The ethanol water consumption is preferably 4~6 times
Column volume, more preferably 5 times.
The present invention does not have special restriction to the concentration step after wash-out, is walked using concentration well known to those skilled in the art
It is rapid.It is thick to be such as concentrated under reduced pressure into eluent, is preferably vacuum dried after the concentration, obtains honeysuckle crude flavonoid powder
Medicinal extract.
The honeysuckle compound beverage that the present invention is provided is thick including the honeysuckle-leaf chlorogenic acid that weight/mass percentage composition is 1~15%
Extract, preferably 5~10%.In the present invention, the preparation method of the honeysuckle-leaf chlorogenic acid crude extract preferably includes following
Step：Honeysuckle-leaf is leached into 5~15h in ethanol water, more preferably 12h regulation pH value is 2~6, with 10~40 times
The boiling water mixing of quality, stirs 5~60min, obtains honeysuckle chlorogenic acid crude extract.
In the present invention, the volume fraction of the leaching ethanol water be preferably 20~90%, more preferably 50~
85%, most preferably 70~80%；The quality of the honeysuckle-leaf is 1kg with the volume ratio of ethanol water：1~6L, it is more excellent
Elect 1kg as：4L.In the present invention, the honeysuckle-leaf is preferably dried in the shade and is crushed, and the particle diameter of the crushing is preferably 60~
100 mesh, more preferably 80 mesh.
The present invention does not have special restriction to the adjusting method of the pH value, using eating that those skilled in the art commonly use
Level soda acid is adjusted, such as acetic acid, aluminium hydroxide, and the pH value is more preferably 3~4；In the present invention, the boiling water
Consumption be more preferably 20~25 times of honeysuckle-leaf quality；The time of the stirring is preferably 45~55min.In the present invention
In, filter operation is preferably carried out after the stirring, the filtrate is honeysuckle chlorogenic acid crude extract.
The honeysuckle compound beverage that the present invention is provided includes the bifidus factor that weight/mass percentage composition is 1~5%, preferably 2
~3.5%.The present invention originates without special restriction to the bifidus factor, using bifid well known to those skilled in the art
The commercially available prod of the factor.
The honeysuckle compound beverage provided in the present invention includes the vitamin C that weight/mass percentage composition is 0.02~0.05%,
Preferably 0.03~0.04%.The present invention to it is described it is ascorbic source without special restriction, using those skilled in the art
Well known ascorbic commercially available prod.
The honeysuckle compound beverage that the present invention is provided includes the white granulated sugar that weight/mass percentage composition is 5~20%, preferably
10%.The present invention originates without special restriction to the white granulated sugar, using white granulated sugar city well known to those skilled in the art
Sell product.The present invention does not have special restriction to the pol of the honeysuckle compound beverage, selects those skilled in the art
The conventional pol of well known beverage is defined.
In the present invention, the raw material of the honeysuckle compound beverage includes the water of surplus, and the water is preferably drinking water.
Present invention also offers the preparation method of honeysuckle compound beverage described in above-mentioned technical proposal, comprise the following steps：
By honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract, honeysuckle Thick many candies, honeysuckle-leaf chlorogenic acid crude extract, double
The mixing of the discrimination factor, vitamin C, white granulated sugar and water, stands and is no less than 24h, is filtrated to get honeysuckle compound beverage.
Time of repose of the invention is preferably 24~48h, more preferably 30h；In the present invention, supernatant is taken after the standing
Carry out follow-up filter operation.
In the present invention, the aperture of the filtering filter membrane is 0.2~0.45 μm.
A kind of honeysuckle compound beverage provided the present invention with reference to specific embodiment and preparation method thereof is done into one
The detailed introduction of step, technical scheme includes but is not limited to following examples.
Embodiment 1
The preparation of honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract and honeysuckle Thick many candies
1) it is 1kg according to mass volume ratio by honeysuckle and water：25L mixes, and air-distillation 3h obtains honeysuckle distillate
And vinasse；
2) by volume fraction in 95% ethanol water is added to vinasse precipitation, to ethanol in mixed solution
In volume fraction be 85%, be filtrated to get alcohol hypostasis and filtrate, the alcohol hypostasis is honeysuckle Thick many candies；
3) by the step 2) filtrate that is filtrated to get adsorbed using macroreticular resin D101, use volume fraction for
40% ethanol water is eluted, and the flow velocity of eluent is 4BV/h, and the consumption of ethanol water is 5 times of column volumes, concentration
Honeysuckle crude flavonoid powder medicinal extract is obtained after drying.
The preparation of honeysuckle-leaf chlorogenic acid crude extract
Honeysuckle-leaf is leached into 6h in ethanol water, regulation pH value is 3, and the boiling water with 20 times of quality mixes, stirring
30min, obtains honeysuckle chlorogenic acid crude extract.
Embodiment 2
The preparation of honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract and honeysuckle Thick many candies
1) it is 1kg according to mass volume ratio by honeysuckle and water：10L mixes, air-distillation 10h, obtains honeysuckle distillation
Liquid and vinasse；
2) by volume fraction in 96% ethanol water is added to vinasse precipitation, to ethanol in mixed solution
In volume fraction be 85%, be filtrated to get alcohol hypostasis and filtrate, the alcohol hypostasis is honeysuckle Thick many candies；
3) by the step 2) filtrate that is filtrated to get adsorbed using macroreticular resin HPD-450, using volume fraction
Ethanol water for 30% is eluted, and the flow velocity of eluent is 5BV/h, and the consumption of ethanol water is 6 times of column volumes, dense
Contracting obtains honeysuckle crude flavonoid powder medicinal extract after drying.
The preparation of honeysuckle-leaf chlorogenic acid crude extract
Honeysuckle-leaf is leached into 15h in ethanol water, regulation pH value is 2.5, and the boiling water with 25 times of quality mixes, and stirs
40min is mixed, honeysuckle chlorogenic acid crude extract is obtained.
Embodiment 3
The preparation of honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract and honeysuckle Thick many candies
1) it is 1kg according to mass volume ratio by honeysuckle and water：30L mixes, and air-distillation 5h obtains honeysuckle distillate
And vinasse；
2) by volume fraction in 90% ethanol water is added to vinasse precipitation, to ethanol in mixed solution
In volume fraction be 85%, be filtrated to get alcohol hypostasis and filtrate, the alcohol hypostasis is honeysuckle Thick many candies；
3) by the step 2) filtrate that is filtrated to get adsorbed using macroreticular resin D101, use volume fraction for
50% ethanol water is eluted, and the flow velocity of eluent is 3BV/h, and the consumption of ethanol water is 4 times of column volumes, concentration
Honeysuckle crude flavonoid powder medicinal extract is obtained after drying.
The preparation of honeysuckle-leaf chlorogenic acid crude extract
Honeysuckle-leaf is leached into 12h in ethanol water, regulation pH value is 4, and the boiling water with 30 times of quality mixes, stirring
20min, obtains honeysuckle chlorogenic acid crude extract.
Embodiment 4
Honeysuckle distillate that mass fraction is 60%, 5% honeysuckle crude flavonoid powder medicinal extract, 15% honeysuckle is slightly more
Sugar, 12% honeysuckle-leaf chlorogenic acid crude extract, 3% bifidus factor, 0.03% vitamin C, 5% white granulated sugar and surplus
Water mixing, stand 24h, use aperture to obtain honeysuckle compound beverage for 0.2 μm of membrane filtration.
Embodiment 5
Honeysuckle distillate that mass fraction is 65%, 6% honeysuckle crude flavonoid powder medicinal extract, 5% honeysuckle is slightly more
Sugar, 10% honeysuckle-leaf chlorogenic acid crude extract, 4% bifidus factor, 0.04% vitamin C, 6% white granulated sugar and surplus
Water mixing, stand 30h, use aperture to obtain honeysuckle compound beverage for 0.45 μm of membrane filtration.
Embodiment 6
Honeysuckle distillate that mass fraction is 70%, 5% honeysuckle crude flavonoid powder medicinal extract, 2% honeysuckle is slightly more
Sugar, 5% honeysuckle-leaf chlorogenic acid crude extract, 3% bifidus factor, 0.05% vitamin C, 8% white granulated sugar and surplus
Water mixing, stand 40h, use aperture to obtain honeysuckle compound beverage for 0.45 μm of membrane filtration.
Embodiment 6
Sample preparation：
The pure water of 65% honeysuckle distillate, bifidus factor 2%, 0.03% vitamin C, 5% white granulated sugar and surplus is adjusted
Prepare and mix, stand cooling precipitation 24h, sample 1 is obtained with filling, sterilizing is carried out after micro-filtrate membrane filtration.
65% honeysuckle distillate, 10% honeysuckle-leaf chlorogenic acid crude extract, bifidus factor 2%, 0.03% vitamin
C, 5% white granulated sugar and surplus pure water allotment system mix, stand cooling precipitation 24h, with carried out after micro-filtrate membrane filtration it is filling, go out
Bacterium is obtained sample 2.
65% honeysuckle distillate, 12% honeysuckle crude flavonoid powder medicinal extract, 8% Thick many candies, bifidus factor 2%, 0.03% dimension
The pure water allotment system of raw element C, 5% white granulated sugar and surplus is mixed, and is stood cooling and is precipitated 24h, with being filled after micro-filtrate membrane filtration
Dress, sterilizing are obtained sample 3.
65% honeysuckle distillate, 10% honeysuckle-leaf chlorogenic acid crude extract, 12% honeysuckle crude flavonoid powder medicinal extract, 5%
Honeysuckle Thick many candies, bifidus factor 2%, 0.03% vitamin C, the allotment system such as 5% white granulated sugar and pure water is mixed, and stands cooling
Precipitation 24h, sample 4 is obtained with filling, sterilizing is carried out after micro-filtrate membrane filtration.
Bacteriostatic test：
The preparation of bacteria suspension：By the Escherichia coli (Gram-negative bacteria) of Refrigerator store and aerobacteria (Gram-positive
Bacterium) transposing in beef extract-peptone fluid nutrient medium, is placed in 37 DEG C of isothermal vibration incubators and cultivates 12h, inhaled with liquid-transfering gun
1mL bacterium solutions are taken in the test tube equipped with 9mL sterilized waters, concussion is uniform, is made bacteria suspension, and every 10 times of dilutions are once dilute step by step
Release, the μ L of concentration 100 for taking (10-5,10-6,10-7) do coating experiment, and parallel three groups of each gradient is not added with any sample completely
It is 3 flat boards of complete blank of LB culture mediums, adjusts bacteria suspension concentration, determines its bacterium solution with flat-plate bacterial colony method dense
Degree, makes its mycetome number be 102Cfu/mL, as examination bacteria suspension.
Bacteriostasis preliminary screening：Sterilizing solid medium is heated to fusing, when being cooled to 50 DEG C, the culture per 20mL
Base is poured into sterile petri dish.After flat board dries naturally, 0.1mL testing samples are pipetted respectively, 0.1mL supplies examination bacteria suspension,
Even to coat on sample-adding flat board, each sample processes 3 repetitions.(being inverted again after coating 30min) separately sets control group, applies bacterium.On
State operation is carried out in superclean bench.By ready-made Bacterial Plate in 37 DEG C of incubated 24h, bacterium colony number is counted.
1 four kinds of sample antibacterial situations of table
4 kinds of fungistatic effects of sample as shown in table 1, are as a result proved：1~4 pair of Gram-negative bacteria of sample and gram sun
Property bacterium has bacteriostasis, but the fungistatic effect of sample 4 is most strong.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of honeysuckle compound beverage, it is characterised in that the component including following weight/mass percentage composition：
Honeysuckle distillate 60~80%, honeysuckle crude flavonoid powder medicinal extract 5~15%, honeysuckle Thick many candies 1~15%, honeysuckle-leaf
Chlorogenic acid crude extract 1~15%, bifidus factor 1~5%, vitamin C 0.02~0.05%, white granulated sugar 5~20% is balance of
Water.
2. composite beverage according to claim 1, it is characterised in that the honeysuckle distillate, the leaching of honeysuckle crude flavonoid powder
The preparation method of cream and honeysuckle Thick many candies is comprised the following steps：
1) it is 1kg according to mass volume ratio by honeysuckle and water：(5~30) L mixes, and air-distillation obtains honeysuckle distillate
And vinasse；
2) vinasse is precipitated using ethanol water, is filtrated to get alcohol hypostasis and filtrate, the alcohol hypostasis is gold and silver
Flower Thick many candies；
3) by the step 2) filtrate that obtains adsorbed using macroreticular resin, eluted using ethanol water, will be washed
Honeysuckle crude flavonoid powder medicinal extract is obtained after de- liquid concentration.
3. composite beverage according to claim 2, it is characterised in that the step 1) distillation time be no less than 1h.
4. composite beverage according to claim 2, it is characterised in that the step 3) ethanol water in ethanol body
Fraction is 30~50%, and elution flow rate is 3~5BV/h, and ethanol water consumption is 4~6 times of column volumes.
5. composite beverage according to claim 1, it is characterised in that the preparation side of the honeysuckle-leaf chlorogenic acid crude extract
Method is comprised the following steps：
Honeysuckle-leaf is leached into 5~15h in ethanol water, regulation pH value is 2~6, with 10~40 times of quality of honeysuckle-leaf
Boiling water mixing, stir 5~60min, obtain honeysuckle chlorogenic acid crude extract.
6. composite beverage according to claim 5, it is characterised in that the quality of the honeysuckle-leaf and ethanol water
Volume ratio is 1kg：(1~6) L.
7. the composite beverage according to claim 5 or 6, it is characterised in that the volume integral of ethanol in the ethanol water
Number is 20~90%.
8. the preparation method of honeysuckle compound beverage described in any one of claim 1~7, comprises the following steps：
By the honeysuckle distillate, honeysuckle crude flavonoid powder medicinal extract, honeysuckle Thick many candies, honeysuckle-leaf chlorogenic acid crude extract, double
The mixing of the discrimination factor, vitamin C, white granulated sugar and water, stands and is no less than 24h, is filtrated to get honeysuckle compound beverage.
9. preparation method according to claim 8, it is characterised in that the aperture of the filtering filter membrane is 0.2~0.45 μ
m。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108771066A (en) * | 2018-05-21 | 2018-11-09 | 遵义市郎笑笑食品有限责任公司 | A kind of honeysuckle beverage processing technology |
| CN109953240A (en) * | 2019-03-13 | 2019-07-02 | 黄冈师范学院 | Clearing away heat and removing summer drink is prepared using honeysuckle distillate and fermentation liquid |
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|---|---|---|---|---|
| CN101785568A (en) * | 2010-03-24 | 2010-07-28 | 牟一心 | Honeysuckle leaf distillate beverage and preparation method thereof |
| CN102935102A (en) * | 2012-10-19 | 2013-02-20 | 湖北楚天舒药业有限公司 | Method for simultaneously producing honeysuckle flower distillate and various active substances |
| CN105713058A (en) * | 2015-12-09 | 2016-06-29 | 湖北楚天舒药业有限公司 | Method for synchronously preparing chlorogenic acid and cynaroside from Lonicera japonica leaves |
| CN106173729A (en) * | 2016-07-12 | 2016-12-07 | 湖北楚天舒药业有限公司 | A kind of Flos Lonicerae compound beverage and preparation method thereof |
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2016
- 2016-12-28 CN CN201611234272.3A patent/CN106858234A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785568A (en) * | 2010-03-24 | 2010-07-28 | 牟一心 | Honeysuckle leaf distillate beverage and preparation method thereof |
| CN102935102A (en) * | 2012-10-19 | 2013-02-20 | 湖北楚天舒药业有限公司 | Method for simultaneously producing honeysuckle flower distillate and various active substances |
| CN105713058A (en) * | 2015-12-09 | 2016-06-29 | 湖北楚天舒药业有限公司 | Method for synchronously preparing chlorogenic acid and cynaroside from Lonicera japonica leaves |
| CN106173729A (en) * | 2016-07-12 | 2016-12-07 | 湖北楚天舒药业有限公司 | A kind of Flos Lonicerae compound beverage and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108771066A (en) * | 2018-05-21 | 2018-11-09 | 遵义市郎笑笑食品有限责任公司 | A kind of honeysuckle beverage processing technology |
| CN109953240A (en) * | 2019-03-13 | 2019-07-02 | 黄冈师范学院 | Clearing away heat and removing summer drink is prepared using honeysuckle distillate and fermentation liquid |
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Application publication date: 20170620 |