CN106830238A - A kind of magnetic polystyrene Compound paint flocculant with bactericidal action - Google Patents

A kind of magnetic polystyrene Compound paint flocculant with bactericidal action Download PDF

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Publication number
CN106830238A
CN106830238A CN201611143505.9A CN201611143505A CN106830238A CN 106830238 A CN106830238 A CN 106830238A CN 201611143505 A CN201611143505 A CN 201611143505A CN 106830238 A CN106830238 A CN 106830238A
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China
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strain
flocculant
waste water
magnetic
inoculated
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CN201611143505.9A
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Chinese (zh)
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戴宇钧
祝盆鑫
祝秀凤
汪鹏程
孙巨福
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天长市银狐漆业有限公司
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Priority to CN201611143505.9A priority Critical patent/CN106830238A/en
Publication of CN106830238A publication Critical patent/CN106830238A/en

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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/52Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02Making microcapsules or microballoons
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/06Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising oxides or hydroxides of metals not provided for in group B01J20/04
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28009Magnetic properties
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28016Particle form
    • B01J20/28021Hollow particles, e.g. hollow spheres, microspheres or cenospheres
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00Specific treatment goals
    • C02F2303/04Disinfection

Abstract

The invention discloses a kind of magnetic polystyrene Compound paint flocculant with bactericidal action, comprise the following steps:(1)Strain separating is purified,(2)Bacteria selection,(3)The fermented and cultured of strain, the preparation of (4) composite magnetic microballoon;Stable preparation process of the present invention, cheap, non-secondary pollution, with good environment protecting, flocculating effect is good, paints slag easy-clear, and blocking corrosion-free to equipment, it is ensured that continuous production, practicality is good.

Description

A kind of magnetic polystyrene Compound paint flocculant with bactericidal action

Technical field

It is multiple the present invention relates to Paint flocculant technical field, more particularly to a kind of magnetic polystyrene with bactericidal action Close Paint flocculant.

Background technology

With the development of the industries such as automobile industry and furniture manufacturing industry, the consumption of spray paint is more and more, therefore has The coating cloud that can not be much coated on workpiece just turns into " cross and spray " coating, crosses discharge rate and reaches 25-60%.Containing big in these coating Amount volatile organic solvent, endanger the health of the mankind, but also environment and air around severe contamination.Spraying is to apply Paint under pressure or centrifugal action, is uniformly dispersed into tiny particle, most by material by spray gun or dish-style atomizer Coating cloud particle is just applied to the process of body surface afterwards.The coating cloud for crossing spray normally enters recirculated water, by the tank of indoor and outdoor Take recirculated water out of outdoor, thus staff's body harm can be reduced, and water can be recycled." cross spray " coating by It is just very big in the viscosity of itself, therefore will stick on the wall of tank, water pipe and water pump, through accumulating over a long period, will be formed compared with Big paint block causes the line clogging, thus the operating efficiency of the circulatory system will to reduce.Mechanical curettage method, chemical agent absorption are solidifying Poly- partition method and biology say that solution etc. is the most frequently used several method of spray painting room processing cycle water, wherein being being widely used Learn coacervation.Due to the long-term addition of chemical type flocculating agent, content of organics is progressively accumulated in causing recirculated water, and COD progressively rises Height, water quality is deteriorated therewith, causes the recirculated water replacement cycle to shorten, and the sediment in waste water resource, and circulation is too many, Solid waste amount is big, and processes solid waste now and be mainly by burning and the method for filling, therefore increased the pollution to environment, non- But the pollution problem of paint slag is fundamentally solved, the burden of environment is also add, therefore research and develop green non-poisonous dirt The small flocculant of dye, can not only be greatly lowered solid waste amount, and can be with reduces cost.

Microbial flocculant is biodegradable by the safety non-toxic of output in various microorganism growth process Natural products, it can make the flocculation such as somatic cells in water, suspended particulate and colloidal particle, precipitation, with good ring Border compatibility.Yang Shuanshuan exists《Flocculation of the microbial flocculant to paint waste water is studied》Pass through a series of bolter in one text Choosing, obtains one plant of preferably flocculation bacterial strain FL-2, single factor experiment is carried out by bacterial strain FL-2, it is determined that optimal condition of culture 30 DEG C, pH is 9, shaking speed is 160r/min, in the composition of culture medium charcoal source be glucose, mixed nitrogen be sodium nitrate, Ammonium chloride and urea, inorganic salts are magnesium sulfate, and good flocculating effect is reached when being compounded with bacillusmusilaginosiengineering, but Effect when two kinds of microbial bacterias are compounded with inorganic flocculating agent is bad.A series of bacterium for above being obtained by separation screening steps Strain FL -2 have good flocculating effect, but from Large-scale culture conditions, it is relatively costly to be substituted, it is necessary to find Charcoal source and the culture medium of nitrogen source culture, reduces cost, are advantageously implemented economic benefit, and control is needed when paint waste water is processed The addition sequence of the amount and compounding strain for adding is made, complex steps are caused, cost is improved, can will by bio-immobilized technology Strain is fixed on carrier, is realized continuous operation, is reduced the purpose of production cost, reaches economic benefit and environmental benefit doulbe-sides' victory Effect.

The content of the invention

The object of the invention is exactly to make up the defect of prior art, there is provided a kind of magnetic polyphenyl second with bactericidal action Alkene Compound paint flocculant.

The present invention is achieved by the following technical solutions:

A kind of magnetic polystyrene Compound paint flocculant with bactericidal action, comprises the following steps:

(1)Strain separating is purified

The paint waste water for processing later stage is fitted into aseptic triangular flask, 4 DEG C of preservations are placed in, seed culture is based on 121 DEG C Lower sterilizing, takes 100 μ L waste water and is inoculated into the seed culture medium of 100ml after cooling, in 37 DEG C of constant temperature Amplification Culture 24h, then Nutrient solution is diluted to 10-1,10-2,10-3,10-4,10-5,10-6 times, then the bacteria suspension coating by each after diluting respectively In on isolation medium, 37 DEG C of incubated 24h choose the good bacterium colony of form and complete initial gross separation, then will be singled out each Bacterium is inoculated in seed culture medium, 37 DEG C of incubated 24h, line is diluted and carried out to the nutrient solution of each bacterium and is isolated and purified, The purifying single bacterium colony that will be finally obtained is stored on isolation medium;

(2)Bacteria selection

By step(1)The bacterium separated is inoculated in seed culture medium with oese respectively, in 30 DEG C, the condition of 160r/min Lower culture 72h, the flocculating rate of strain is tested with the aqueous suspension ofkaolin of 4g/L, and the strain by flocculating rate more than 50% retains, and Repeatedly passed on, the culture presevation that will be filtered out;

(3)The fermented and cultured of strain

A, beer waste water and sugaring fermentation waste water are centrifuged 20min under 5000rpm respectively, suspended impurity are removed, wherein refining sugar Fermentation waste water total sugar content is 19.85g/L, and total nitrogen content is 3263.3mg/L, and beer waste water total sugar content is 196.1g/L, always Nitrogen content is 1796.8mg/L, and then according to total sugar concentration 7g/L, charcoal source and nitrogen source compare 50:1 is diluted, and adds phosphoric acid Sodium dihydrogen and disodium hydrogen phosphate, wherein phosphate dihydrogen sodium concentration are 2g/L, and disodium hydrogen phosphate concentration is 5g/L, adds magnesium sulfate The pH for adjusting fermentation medium is 9, and autoclaving is standby;

B, by step(2)In be deposited in the producing strains strain on inclined-plane and be inoculated into fermentation medium, in 30 DEG C, 150r/min constant temperature 3h is cultivated on oscillator, each bacterial strain is in exponential phase, be made strain suspension, be inoculated with according to the inoculum concentration of 5-10% In the fermentation medium prepared to 100ml steps a, 72h is cultivated under the conditions of 30 DEG C, rotating speed are for 160r/min;

C, zymotic fluid obtained in step b is placed on 4 DEG C of high speed freezing centrifuges in 8000r/min be centrifuged 30-40min, will To supernatant in add 2 times amount absolute ethyl alcohols stand 24h at 4 DEG C, then proceed under the conditions of 4 DEG C in 8000r/min Centrifugation 30-40min, the precipitation that will be obtained absolute ethyl alcohol cyclic washing is placed in the 48h that dialysed in bag filter, after freeze-drying Microbial flocculant;

(4) preparation of composite magnetic microballoon

D, by ferric chloride hexahydrate and Iron dichloride tetrahydrate according to ferric iron and ferrous iron mol ratio 1:1 mixing, electric stirring It is fully mixed, be slowly added dropwise the NaOH of 1mol/L while stirring at 30 DEG C up to the pH of solution is 11, heating makes Its reaction temperature is controlled at 60 DEG C, is then added dropwise over oleic acid stirring reaction 1-1.5h, and reaction is cooled to room temperature after terminating, then With distilled water cyclic washing in neutrality, ground after vacuum drying standby;

E, the product prepared in above-mentioned steps d, dodecyl sodium sulfate, distilled water are sequentially added ultrasonic disperse in container, By emulsifying mixture 30min under the protection of nitrogen, heating water bath is warming up to 70 DEG C, the potassium peroxydisulfate of 1/4 volume is added, by residue Potassium peroxydisulfate, the microbial flocculant mixing for preparing of divinylbenzene, acrylic acid, styrene, soluble starch and step c, Mixture is obtained after stirring, when being continuously heating to 75 DEG C, mixture isothermal reaction 8-10h is added dropwise over, after reaction terminates Being cooled to room temperature carries out Magneto separate, and the product hydrochloric acid solution of 1mol/L soaks 48h, and is alternately washed with absolute ethyl alcohol and distilled water Wash for several times, be vacuum dried to obtain magnetic composite microsphere, then the magnetic composite microsphere is impregnated into the dodecyl dimethyl of 1.5-2% In Benzylmagnesium chloride ammonium salt solution, filtered after ultrasonic vibration 10-15min, dried.

The formulation ratio of described isolation medium is:Beef extract 3-4g/L, peptone 10-12g/L, sodium chloride 5-6g/ L, agar 15-20g/L, plus distilled water to 1000ml;To 7.0-7.2, sterilize regulation pH value 20min in 121 DEG C;Seed culture medium Recipe ratio is:Beef extract 3-4g/L, peptone 10-13g/L, sodium chloride 5-7g/L, plus distilled water to 1000ml, regulation pH value to 7-7.2, sterilize 20min in 121 DEG C.

The step(4)In each raw material be according to weight:Ferric chloride hexahydrate 8.2-8.8, oleic acid 0.4-0.6, Potassium peroxydisulfate 0.3-0.4, divinylbenzene 0.02-0.04, styrene 9.2-9.6, acrylic acid 2.2-2.5, starch 0.4-0.5g, Dodecyl benzyl dimethyl ammonium chloride 0.2-0.4, appropriate Iron dichloride tetrahydrate, 1mol/L Sodium hydroxide q. s, 1mol/L Appropriate hydrochloric acid, appropriate absolute ethyl alcohol, microbial flocculant 2.2-2.4, distillation appropriate amount of water.

It is an advantage of the invention that:The present invention from paint waste water screening, extracting and developing, be purified into microbial flocculant produce Raw bacterium, optimization culture improves its flocculation activity, and uses the production of cheap substrate fermented and cultured reduction microbial flocculant Cost, can mass produce culture, and resource to discarding is reused, with good economic benefit and Social benefit, environmental protection, to improve all many conditions limitation when the single use of microbial flocculant or compounding use, the present invention It is set to possess good absorption and magnetic behavior using the magnetic microsphere loading microorganisms flocculant for preparing, and it is easily separated, can Repetitive cycling is utilized, and nano ferriferrous oxide is prepared by chemical coprecipitation, and the magnetic ferroferric oxide surface of preparation has Hydrophily, insoluble in organic solvent, oleic acid one end is hydrophilic group, and the other end is lipophilic group, hydrophilic radical can with hydrophilic Property nano ferriferrous oxide particle surface be connected, exposed to particle surface then be lipophilic group, reach oil loving purpose, and And form good clad on surface, it is to avoid and the reunion between magnetic particle, stability is improved, recycling emulsion polymerization will Microbial flocculant is coated in Magnetic Polystyrene Microsphere, improves resistance to acids and bases, corrosion resistance and compression strength, carries it The flocculation efficiency and service life of microbial flocculant high, Paint flocculant stable preparation process prepared by the present invention, price It is cheap, non-secondary pollution, with good environment protecting, flocculating effect is good, paint slag easy-clear, and blocking corrosion-free to equipment, Continuous production can be ensured, practicality is good.

Specific embodiment

A kind of magnetic polystyrene Compound paint flocculant with bactericidal action, comprises the following steps:

(1)Strain separating is purified

The paint waste water for processing later stage is fitted into aseptic triangular flask, 4 DEG C of preservations are placed in, seed culture is based on 121 DEG C Lower sterilizing, takes 100 μ L waste water and is inoculated into the seed culture medium of 100ml after cooling, in 37 DEG C of constant temperature Amplification Culture 24h, then Nutrient solution is diluted to 10,10,10,10,10,10 times respectively, then each bacteria suspension after diluting is coated into isolation medium On, 37 DEG C of incubated 24h choose the good bacterium colony of form and complete initial gross separation, then each bacterium that will be singled out is inoculated in seed In culture medium, 37 DEG C of incubated 24h dilute and carry out to the nutrient solution of each bacterium line and isolate and purify, by what is finally obtained Purifying single bacterium colony is stored on isolation medium;

(2)Bacteria selection

By step(1)The bacterium separated is inoculated in seed culture medium with oese respectively, in 30 DEG C, the condition of 160r/min Lower culture 72h, the flocculating rate of strain is tested with the aqueous suspension ofkaolin of 4g/L, and the strain by flocculating rate more than 50% retains, and Repeatedly passed on, the culture presevation that will be filtered out;

(3)The fermented and cultured of strain

A, beer waste water and sugaring fermentation waste water are centrifuged 20min under 5000rpm respectively, suspended impurity are removed, wherein refining sugar Fermentation waste water total sugar content is 19.85g/L, and total nitrogen content is 3263.3mg/L, and beer waste water total sugar content is 196.1g/L, always Nitrogen content is 1796.8mg/L, and then according to total sugar concentration 7g/L, charcoal source and nitrogen source compare 50:1 is diluted, and adds phosphoric acid Sodium dihydrogen and disodium hydrogen phosphate, wherein phosphate dihydrogen sodium concentration are 2g/L, and disodium hydrogen phosphate concentration is 5g/L, adds magnesium sulfate The pH for adjusting fermentation medium is 9, and autoclaving is standby;

B, by step(2)In be deposited in the producing strains strain on inclined-plane and be inoculated into fermentation medium, in 30 DEG C, 150r/min constant temperature 3h is cultivated on oscillator, each bacterial strain is in exponential phase, be made strain suspension, be inoculated into according to 5% inoculum concentration In fermentation medium prepared by 100ml steps a, 72h is cultivated under the conditions of 30 DEG C, rotating speed are for 160r/min;

C, zymotic fluid obtained in step b is placed on 4 DEG C of high speed freezing centrifuges in 8000r/min be centrifuged 30min, by what is obtained Add the absolute ethyl alcohol of 2 times of amounts to stand 24h at 4 DEG C in supernatant, then proceed to be centrifuged in 8000r/min under the conditions of 4 DEG C 30min, the precipitation that will be obtained absolute ethyl alcohol cyclic washing, is placed in the 48h that dialysed in bag filter, and microorganism wadding is obtained after freeze-drying Solidifying agent;

(4) preparation of composite magnetic microballoon

D, by ferric chloride hexahydrate and Iron dichloride tetrahydrate according to ferric iron and ferrous iron mol ratio 1:1 mixing, electric stirring It is fully mixed, be slowly added dropwise the NaOH of 1mol/L while stirring at 30 DEG C up to the pH of solution is 11, heating makes Its reaction temperature is controlled at 60 DEG C, is then added dropwise over oleic acid stirring reaction 1h, and reaction is cooled to room temperature after terminating, then with steaming Distilled water cyclic washing grinds standby in neutrality after vacuum drying;

E, the product prepared in above-mentioned steps d, dodecyl sodium sulfate, distilled water are sequentially added ultrasonic disperse in container, By emulsifying mixture 30min under the protection of nitrogen, heating water bath is warming up to 70 DEG C, the potassium peroxydisulfate of 1/4 volume is added, by residue Potassium peroxydisulfate, the microbial flocculant mixing for preparing of divinylbenzene, acrylic acid, styrene, soluble starch and step c, Mixture is obtained after stirring, when being continuously heating to 75 DEG C, mixture isothermal reaction 8h is added dropwise over, reaction is cooled down after terminating Magneto separate is carried out to room temperature, the product hydrochloric acid solution of 1mol/L soaks 48h, and alternately washs number with absolute ethyl alcohol and distilled water It is secondary, magnetic composite microsphere is vacuum dried to obtain, then the magnetic composite microsphere is impregnated into 1.5% dodecyl dimethyl benzyl chloride Change in ammonium salt solution, filtered after ultrasonic vibration 10min, dried.

The formulation ratio of described isolation medium is:Beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, agar 15g/L, plus distilled water to 1000ml;To 7.0, sterilize regulation pH value 20min in 121 DEG C;Seed culture medium recipe ratio is:Beef Cream 3g/L, peptone 10g/L, sodium chloride 5g/L, plus distilled water to 1000ml, regulation pH value to 7, sterilize 20min in 121 DEG C.

The step(4)In each raw material according to weight portion(Kilogram)Match and be:Ferric chloride hexahydrate 8.2, oleic acid 0.4, mistake Potassium sulfate 0.3, divinylbenzene 0.02, styrene 9.2, acrylic acid 2.2, starch 0.4g, dodecyl dimethyl Benzylmagnesium chloride Ammonium 0.2, appropriate Iron dichloride tetrahydrate, 1mol/L Sodium hydroxide q. s, appropriate 1mol/L hydrochloric acid, appropriate absolute ethyl alcohol, micro- life Thing flocculant 2.2, distillation appropriate amount of water.

In order to embody the validity of the performance of the Compound paint flocculant prepared in embodiment, configuration paint waste water enters Row performance verification, process is as follows:

To 100ml cold running waters are added in the open bottles equipped with magnetic stirring bar, it is 12 to adjust pH with the NaOH of 1mol/L, Turn on agitator adds the commercially available automotive paints of 6 drops, is stood after stirring and obtains paint waste water, and concentration is about 30mg/L, to Add the Compound paint flocculant of waste water total amount mass fraction 0.3%, high-speed stirred 1min that knot is observed after standing in paint waste water Really.It has been observed that:Bottle wall does not have obvious bur, and water quality is limpid, and the floccule of formation is compact in cellular and to bubble through the water column, Flocculating rate is 92.3%.

Claims (3)

1. a kind of magnetic polystyrene Compound paint flocculant with bactericidal action, it is characterised in that comprise the following steps:
(1)Strain separating is purified
The paint waste water for processing later stage is fitted into aseptic triangular flask, 4 DEG C of preservations are placed in, seed culture is based on 121 DEG C Lower sterilizing, takes 100 μ L waste water and is inoculated into the seed culture medium of 100ml after cooling, in 37 DEG C of constant temperature Amplification Culture 24h, then Nutrient solution is diluted to 10-1,10-2,10-3,10-4,10-5,10-6 times, then the bacteria suspension coating by each after diluting respectively In on isolation medium, 37 DEG C of incubated 24h choose the good bacterium colony of form and complete initial gross separation, then will be singled out each Bacterium is inoculated in seed culture medium, 37 DEG C of incubated 24h, line is diluted and carried out to the nutrient solution of each bacterium and is isolated and purified, The purifying single bacterium colony that will be finally obtained is stored on isolation medium;
(2)Bacteria selection
By step(1)The bacterium separated is inoculated in seed culture medium with oese respectively, in 30 DEG C, the condition of 160r/min Lower culture 72h, the flocculating rate of strain is tested with the aqueous suspension ofkaolin of 4g/L, and the strain by flocculating rate more than 50% retains, and Repeatedly passed on, the culture presevation that will be filtered out;
(3)The fermented and cultured of strain
A, beer waste water and sugaring fermentation waste water are centrifuged 20min under 5000rpm respectively, suspended impurity are removed, wherein refining sugar Fermentation waste water total sugar content is 19.85g/L, and total nitrogen content is 3263.3mg/L, and beer waste water total sugar content is 196.1g/L, always Nitrogen content is 1796.8mg/L, and then according to total sugar concentration 7g/L, charcoal source and nitrogen source compare 50:1 is diluted, and adds phosphoric acid Sodium dihydrogen and disodium hydrogen phosphate, wherein phosphate dihydrogen sodium concentration are 2g/L, and disodium hydrogen phosphate concentration is 5g/L, adds magnesium sulfate The pH for adjusting fermentation medium is 9, and autoclaving is standby;
B, by step(2)In be deposited in the producing strains strain on inclined-plane and be inoculated into fermentation medium, in 30 DEG C, 150r/min constant temperature 3h is cultivated on oscillator, each bacterial strain is in exponential phase, be made strain suspension, be inoculated with according to the inoculum concentration of 5-10% In the fermentation medium prepared to 100ml steps a, 72h is cultivated under the conditions of 30 DEG C, rotating speed are for 160r/min;
C, zymotic fluid obtained in step b is placed on 4 DEG C of high speed freezing centrifuges in 8000r/min be centrifuged 30-40min, will To supernatant in add 2 times amount absolute ethyl alcohols stand 24h at 4 DEG C, then proceed under the conditions of 4 DEG C in 8000r/min Centrifugation 30-40min, the precipitation that will be obtained absolute ethyl alcohol cyclic washing is placed in the 48h that dialysed in bag filter, after freeze-drying Microbial flocculant;
(4) preparation of composite magnetic microballoon
D, by ferric chloride hexahydrate and Iron dichloride tetrahydrate according to ferric iron and ferrous iron mol ratio 1:1 mixing, electric stirring It is fully mixed, be slowly added dropwise the NaOH of 1mol/L while stirring at 30 DEG C up to the pH of solution is 11, heating makes Its reaction temperature is controlled at 60 DEG C, is then added dropwise over oleic acid stirring reaction 1-1.5h, and reaction is cooled to room temperature after terminating, then With distilled water cyclic washing in neutrality, ground after vacuum drying standby;
E, the product prepared in above-mentioned steps d, dodecyl sodium sulfate, distilled water are sequentially added ultrasonic disperse in container, By emulsifying mixture 30min under the protection of nitrogen, heating water bath is warming up to 70 DEG C, the potassium peroxydisulfate of 1/4 volume is added, by residue Potassium peroxydisulfate, the microbial flocculant mixing for preparing of divinylbenzene, acrylic acid, styrene, soluble starch and step c, Mixture is obtained after stirring, when being continuously heating to 75 DEG C, mixture isothermal reaction 8-10h is added dropwise over, after reaction terminates Being cooled to room temperature carries out Magneto separate, and the product hydrochloric acid solution of 1mol/L soaks 48h, and is alternately washed with absolute ethyl alcohol and distilled water Wash for several times, be vacuum dried to obtain magnetic composite microsphere, then the magnetic composite microsphere is impregnated into the dodecyl dimethyl of 1.5-2% In Benzylmagnesium chloride ammonium salt solution, filtered after ultrasonic vibration 10-15min, dried.
2. a kind of magnetic polystyrene Compound paint flocculant with bactericidal action according to claim 1, its feature exists In the formulation ratio of described isolation medium is:Beef extract 3-4g/L, peptone 10-12g/L, sodium chloride 5-6g/L, agar 15-20g/L, plus distilled water to 1000ml;To 7.0-7.2, sterilize regulation pH value 20min in 121 DEG C;Seed culture medium recipe ratio For:Beef extract 3-4g/L, peptone 10-13g/L, sodium chloride 5-7g/L, plus distilled water to 1000ml, regulation pH value to 7-7.2, Sterilize 20min in 121 DEG C.
3. a kind of magnetic polystyrene Compound paint flocculant with bactericidal action according to claim 1, its feature exists In the step(4)In each raw material be according to weight:Ferric chloride hexahydrate 8.2-8.8, oleic acid 0.4-0.6, persulfuric acid Potassium 0.3-0.4, divinylbenzene 0.02-0.04, styrene 9.2-9.6, acrylic acid 2.2-2.5, starch 0.4-0.5g, dodecane Base dimethyl benzyl ammonium chloride 0.2-0.4, appropriate Iron dichloride tetrahydrate, 1mol/L Sodium hydroxide q. s, 1mol/L hydrochloric acid are fitted Amount, appropriate absolute ethyl alcohol, microbial flocculant 2.2-2.4, distillation appropriate amount of water.
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