CN106754352A - A kind of disposable de- magnetic sorting apparatus and its method for separating - Google Patents
A kind of disposable de- magnetic sorting apparatus and its method for separating Download PDFInfo
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- CN106754352A CN106754352A CN201710155065.7A CN201710155065A CN106754352A CN 106754352 A CN106754352 A CN 106754352A CN 201710155065 A CN201710155065 A CN 201710155065A CN 106754352 A CN106754352 A CN 106754352A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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Abstract
The invention discloses the disposable de- magnetic sorting apparatus of one kind, the device includes magnetic core and the capture observation window being arranged at outside magnetic core, the capture observation window is made up of magnetic-permeable material, and the capture observation window is unique regional transmission in the magnetic field that magnetic core sends, the magnetic field sent by magnetic core is sent out by capturing observation window, for the target particle containing magnetic in adsorptive liquid sample, and the capture observation window is detachable.For sorting during the target particles containing magnetic in fluid sample, target particles are adsorbed on the surface of capture observation window in the magnetic field distributed by magnetic core, and without wash-out, the capture observation window only need to be removed the analysis and research that the object of capture just can be directly applied to next step, save operating procedure, the loss of object is reduced, and operating method is convenient, is highly suitable for the sorting of rare sample in various biological samples.
Description
Technical field
Caught suitable for rare cell the present invention relates to the disposable de- magnetic sorting apparatus of one kind and its method for separating, especially one kind
The disposable de- magnetic sorting apparatus and its method for separating for obtaining.
Background technology
Immunomagnetic enrichment can be combined with the specific monoclonal antibody for being connected with magnetic bead using cell surface antigen, additional
In the presence of magnetic field, be attracted by the cell that antibody is connected with magnetic bead and be trapped in magnetic field, and other cells because without
Magnetic, it is impossible to stopped in magnetic field, so that cell is separated.Wherein, more representative method is magnetic cell sorting
System (magnetic activated cell sorting system, MACS), after birth or intracellular are demarcated using immunomagnetic beads
Cellular antigens, then captured by external magnetic field and target cell marked by immunomagnetic beads.
Circulating tumor cell (CTC) refers to the tumour cell for having primary tumo(u)r or neoplasm metastasis to be released into peripheral blood,
It is extremely rare in healthy human body.According to the literature, circulating tumor cell be released into blood by malignant tumour forms early stage.Follow
Ring tumour cell discharges into blood circulation by primary tumor, is the committed step of Malignant Tumor Recurrence and transfer.Therefore, CTC inspections
Survey is considered as a kind of new diagnostic techniques, new prognostic evaluation instrument, effective treating malignant tumor should
Prediction index, is sent to high expectation in clinical practice.
But because content of the CTC cells in peripheral blood is few, it is necessary to improve the sensitive of detection by enrichment of cell
Degree.A kind of common method in CTC beneficiation technologies is exactly the technology with immunomagnetic enrichment as core, current commercialized CTC
Enrichment and detection technique, such as CellSearch from EpCAM marked by magnetic bead CTC and carry out Magnetic screen choosing, but its absorption magnetic bead
Magnet be arranged on outside sample tube, therefore invisible spectro Magnetic field inhomogeneity, magnetic field intensity increase with magnetic fields distance and
Decay is notable, causes that the magnetic force of one end away from magnet is almost nil, and the target cell positioned at the end is easily inhaled and lost by leakage.
In addition the further cumbersome liquid concentration step of cell needs and transfer step for being adsorbed capture could be used for next step
Analysis and research.
The method of another kind absorption magnetic bead is that, using the invisible spectro magnetic bar of insertion, the bottom surface of magnetic bar has magnetic, though
Right this method can greatly improve magnetic field adsorption efficiency, but the shortcoming of the method is to have adsorbed that cell also is eluted into ability
Carry out the operation of next step.
Therefore, those skilled in the art be devoted to exploitation one kind can produce uniform magnetic field, can quickly, completely, concentrate suction
Attached magnetic bead, while the magnetic sorting apparatus of next step analysis and operation can be carried out without wash-out.
The content of the invention
In view of the drawbacks described above of prior art, the technical problems to be solved by the invention are to provide a kind of without eluting i.e.
The magnetic sorting apparatus of downstream analysis can be analyzed and be applied to, the disposable de- magnetic sorting apparatus include magnetic core and are arranged at outside magnetic core
Capture observation window, the capture observation window is made up of magnetic-permeable material, and the capture observation window is the unique of the magnetic field that magnetic core sends
Regional transmission, the magnetic field sent by magnetic core is sent out by capturing observation window, and magnetic is contained for the target in adsorptive liquid sample
Particle, and the capture observation window is detachable.
Preferably, the capture observation window is plane.
Preferably, the capture observation window is made of clear material, such as glass；More preferably with anti-skidding anticreep
The transparent material of the property that falls is made, the target substance that can adhere to well in sample, prevents from losing behind magnetic field target substance from institute
Capture observation window surface is stated to come off.
In the preferred embodiment of the present invention, the size of the capture observation window in above-mentioned disposable de- magnetic sorting apparatus
Can as needed be designed with shape, so that target substance can be concentrated in less region, realize efficient, the concentration of target substance
Capture and exempt from elution analysis.The capture observation window can be arranged on institute by the various mechanisms such as spring, inserted link, buckle, screw thread
State the surface of magnetic core.
Further, the disposable de- magnetic sorting apparatus also include inserted link, and the magnetic core is arranged at one end of the inserted link,
The inserted link is used for taking and pulling and pushing for the magnetic core.
Preferably, the disposable de- magnetic sorting apparatus also include epitheca, and the epitheca is removably sheathed on the magnetic core
Outside, the capture observation window is arranged on the epitheca.
Further, the magnet for producing magnetic field is provided with the magnetic core, the magnet is by permanent-magnet material or permanent magnetism and soft magnetism group
Condensation material is made；Preferably, the permanent-magnet material is Ru-Fe-Mn permanent magnet.
Preferably, the magnetic core has capture plane, and the capture plane corresponds to the capture observation window, and the magnet is produced
Raw magnetic field is sent by the capture plane, in acting on fluid sample through the capture observation window.
Preferably, the magnet is the surface exposure for corresponding only to the capture plane, and remaining surface is by soft magnetic materials bag
The permanent-magnet material wrapped up in.
In the preferred embodiment of the present invention, the magnet is made up of permanent magnet and soft magnetic materials, the permanent magnetism
Iron is hexahedron, wherein five faces are wrapped up by soft magnetic materials, only exposes a surface corresponding with capture plane.This design can
So that the magnetic field of magnet script increased radioactivity is all deflected to capture plane without loss magnetic field intensity to greatest extent.It is preferred that
Ground, the magnet is enclosed with the permanent magnet group of soft magnetic materials into the exposed surface of many block permanent magnets is consistent towards capture by polylith
Planar alignment；The quantity of the permanent magnet for being enclosed with soft magnetic materials preferably 2-9 blocks.
In one embodiment of the invention, the magnet is a single block permanent magnet or is spelled by 2-9 block permanent magnets
Dress is combined；By the assembled magnet of many block permanent magnets, the relative face of adjacent two block permanent magnet can be different magnetic poles (i.e.
S-N or N-S) or same pole (i.e. S-S or N-N).
In the preferred embodiment of the present invention, the magnet is arranged by the word of permanent magnet one that polylith soft magnetic materials is wrapped up
Row composition, and the magnetic pole of two pieces of permanent magnet iron phases pair close to each other is identical, due to the effect of repelling each other of the magnetic line of force, substantial amounts of magnetic force
Line can be gathered on the good soft magnetic materials of magnetic conductivity, it is magnetized into magnetic pole, so as to extend the length of magnetic pole, using " forever
The combination of magnetic+soft magnetism ", can increase the intensity of magnetic core table magnetic, in the case of being captured especially with regional area, the combination
Mode can be guided to capture region the divergent magnetic field of magnet surrounding, enhance the table magnetic of capture region, reduce leakage field, together
When avoid loss of field.
In the preferred embodiment of the present invention, the magnetic core includes magnet and holding tank, and the magnet is mounted
In the holding tank, the capture plane on the magnetic core surface is arranged to the magnetic field transmissive window on the holding tank, magnet hair
The magnetic field for going out outwards is transmitted by the magnetic field transmissive window, and for the target particle containing magnetic in adsorptive liquid sample, the holding tank is removed
Region beyond the transmissive window of magnetic field is arranged to that magnetic field can not be transmitted.Further, in the preferred embodiment of the present invention,
One side of the holding tank has magnetic field transmissive window.
A kind of better embodiment of the magnetic field transmissive window is hollow out, and this kind of design can make magnetic field straight without any loss ground
Connect and pass, and magnet can be mounted and dismounted conveniently by the window of the hollow out, so as to the replacing and cleaning of magnet；The magnetic field
Another better embodiment of transmissive window is made by can pass through material by magnetic field, and this kind of design may better secure magnet to be prevented
Come off, while can be by the overall dismantled and assembled replacing and cleaning to realize magnet of holding tank.
Further, the other end of the inserted link is used to hold, thus thicker design can increase contact surface, and immerses liquid
The part of sample should be advisable with thinner design, can reduce the spill-out of liquid；And then in one kind side of being preferable to carry out of the invention
In formula, the part of the inserted link immersion fluid sample is thin compared with the magnetic core of one end and the grip portion of the other end.Preferably, it is described
One end away from magnetic core of inserted link has projection, as the screens part of the device, the disposable de- magnetic sorting apparatus is facilitated
Be suspended vacantly on support frame.
In the preferred embodiment of the present invention, the disposable de- magnetic sorting apparatus include magnetic core, inserted link and epitheca,
The epitheca is removably sheathed on the outside of the magnetic core, and with capture observation window, is sent for adsorbing with magnetic core surface
The capture plane in magnetic field of the target containing magnetic particle is corresponding in fluid sample.Magnetic field sends from the capture plane on magnetic core surface, thoroughly
Cross corresponding capture observation window on epitheca and act on fluid sample, so that target particle aggregation containing magnetic is in the capture observation window
Position, while realizing the purpose of capture and the concentration of target substance.The target substance of capture observation window is gathered in without wash-out,
The epitheca need to only be disassembled, you can follow-up various treatment (as dyeed) are easily carried out, in follow-up analysis and research
The capture observation window then can be observed directly under the microscope.
It is further preferred that the epitheca has two relative parallel planes, one is each provided with two planes
Transparent observation window；The observation window of one of plane sends corresponding to magnetic core surface and contains magnetic for target in adsorptive liquid sample
The capture plane in the magnetic field of particle, as the capture observation window；The observation window of another plane then sends magnetic positioned at the magnetic core
The back side of the capture plane of field, is not used in capture target particle containing magnetic, is only used for providing printing opacity in observation.This kind of design can be by
The epitheca for disassembling is placed directly within basis of microscopic observation.
In order to reduce resistance of the disposable de- magnetic sorting apparatus in fluid sample, the magnetic core, holding tank and epitheca
Profile should as far as possible using camber line design, such as in a preferred embodiment of the invention, the epitheca is with through hole
Column；In another preferred embodiment of the invention, the epitheca is the shape of the similar pen cap of an end closure；And for convenience
Observation, the observation window should as far as possible use planar design, and such as in a preferred embodiment of the invention, the epitheca is one
End closure, the similar capsule shape with a pair of parallel plane.
Further, present invention also offers a kind of method for separating of above-mentioned disposable de- magnetic sorting apparatus, including step：
1) by the disposable de- magnetic sorting apparatus insertion containing in fluid sample of the target containing magnetic particle；
2) magnetic core is rotated in the sample and/or is moved up and down and carry out abundant adsorbed target particle containing magnetic, so as to complete
To sorting of the target containing magnetic particle；
3) the described disposable de- magnetic sorting apparatus for completing will be adsorbed to take out and carry out necessary cleaning, dyeing；
4) will dye in the device of the described disposable de- magnetic sorting apparatus immigration follow-up study for completing, extract the magnetic core out,
Leave counting or observational study of the epitheca for next step.
Preferably, step 2) described in rotation include revolve round the sun and rotation.
Preferably, disposable de- magnetic sorting apparatus motion in the sample is driven by machinery.
The disposable de- magnetic sorting apparatus that the present invention is provided have following Advantageous Effects：
1st, by the way of being adsorbed in inserting sample, by rotating, moving up and down the purpose for reaching uniform adsorption, it is to avoid
The problem of non-uniform magnetic field and magnetic field intensity deep fades is produced using sample external magnet in the prior art；
2nd, the present invention is eliminated elution step of the prior art by the design of magnetic core, capture observation window and epitheca,
Flow is simplified, while decreasing the loss of target substance；
3rd, by the way of permanent magnet is staggered and soft material is wrapped up, stabilization improves core portion of the invention
The intensity in magnetic field, with more preferable adsorption effect；
4th, the capture rate of sorting unit of the present invention is high, and can make target magnetic particle aggregation in a less region,
Rare target substance in biological sample is highly suitable for, such as magnetic can be combined by certain special properties in capture fluid sample
The composition of property particle, the especially capture of rare cell.
The technique effect of design of the invention, concrete structure and generation is described further below with reference to accompanying drawing, with
It is fully understood from the purpose of the present invention, feature and effect.
Brief description of the drawings
Fig. 1 is the overall structure diagram of a preferred embodiment of the present invention；
Fig. 2 is the hinge pedestal structure schematic diagram of embodiment in Fig. 1；
Fig. 3 is the magnet positive structure schematic of embodiment in Fig. 1；
Fig. 4 is the epitheca structural representation of embodiment in Fig. 1；
Fig. 5 is a kind of structural representation of preferred embodiment of present invention capture observation window；
Fig. 6 is the overall structure diagram of another preferred embodiment of the invention；
Fig. 7 is the combination magnet arrangement positive structure schematic of embodiment in Fig. 6, and a is consistent magnetic pole, and b is that magnetic pole is opposite；
Fig. 8 is the overall structure diagram of another preferred embodiment of the invention；
Fig. 9 is the magnetic core and epitheca integrated structure schematic diagram of embodiment in Fig. 8；
Figure 10 is the core structure schematic diagram of embodiment in Fig. 8；
Figure 11 is the epitheca positive structure schematic of embodiment in Fig. 8；
Figure 12 is the epitheca overlooking the structure diagram of embodiment in Fig. 8.
Specific embodiment
Embodiment 1
Fig. 1-4 show a structural representation for preferred embodiment of the disposable de- magnetic sorting apparatus of the present invention, the magnetic point
Screening device includes cylindrical inserted link 1, and the bottom of inserted link 1 is provided with magnetic core 2, and the internal package of the magnetic core has magnet, the magnet
It is made up of (Fig. 3) with the soft magnetic materials 22 wrapped up outside it permanent magnet 21, the side surface of the only exposed portion of permanent magnet 21 corresponds to be inserted
Magnetic field transmissive window (rectangular region in Fig. 2) on bar 1 is used for capture of the target containing magnetic particle, and the transmissive window can be hollow out
Design is made of transparent magnetic-permeable material, such as glass；Region of the inserted link 1 in addition to the transmissive window of magnetic field, can using it is transparent, half
Transparent or opaque material is made.
In addition the disposable de- magnetic sorting apparatus also include epitheca 3 (Fig. 4), and the epitheca 3 is enclosed within the outer of inserted link 1 as the pen cap
Portion, detachably.Corresponding to capture observation window 4 is provided with the position of the magnetic field transmissive window of magnetic core on the epitheca 3, the capture is seen
The material of window 4 is examined for glass, magnetic field can pass through the target particle containing magnetic in the adsorptive liquid sample of capture observation window 4, except capture
The material of the epitheca 1 beyond observation window 4 is transparent, translucent or opaque material, and magnetic core is close to capture observation window, captured
Target particle aggregation containing magnetic in capture observation window 4 position, so as to it is follow-up further observational study.
Structure of the invention is not unique, and the mounting means of the capture observation window can also be as shown in figure 5, by being arranged at
State the bindiny mechanism 41 on magnetic core surface, be removably attachable on magnetic core, the connected mode of the bindiny mechanism can be spring,
Inserted link, buckle, screw thread etc.；In addition it is any other using know-why of the invention and realize it is of the invention implementation purpose knot
Structure is all feasible, the size of permanent magnet, volume and Distribution of Magnetic Field mode, type selecting of soft magnetic materials etc. such as in magnetic core.
Embodiment 2
Fig. 6 show the structural representation of another preferred embodiment of the disposable de- magnetic sorting apparatus of the present invention, and this is disposable
De- magnetic sorting apparatus include the inserted link 1 of cylinder, and the bottom of inserted link 1 has magnetic core 2, be provided with the magnetic core four pieces of squares or
The small magnet of cuboid, every piece of magnet is all that the permanent magnet for wrapping up five outer surfaces by soft magnetic materials is constituted, the one side do not wrapped up
Corresponding to the capture observation window 4 in the magnetic field transmissive window and epitheca 3 of magnetic core, and the magnetic pole of two adjacent block permanent magnets is consistent (such as
It is N-S-N-S-N-S-N-S in Fig. 7 a) or it is opposite (being N-S-S-N-N-S-S-N in such as Fig. 7 b)；In addition the inserted link 1 is close upper
End position has raised 5, as screens part device can be enable easily to be suspended on support frame.Magnet arrangement of the invention
Mode is not unique, and any arrangement mode that can meet magnetic requirements is all feasible.
Embodiment 3
Fig. 8 show the structural representation of another preferred embodiment of magnetic sorting apparatus of the present invention, the wherein bottom of inserted link 1
End is connected with magnetic core 2, and the upper end 11 of inserted link 1 is thick cylinder, can be easy to grip, and the stage casing 12 of inserted link 1 is thin cylindric, when this
When in device insertion fluid sample, this kind of design can reduce the spilling of liquid.
Fig. 9 show the schematic diagram of magnetic core 2 when the present embodiment is cased with epitheca 3, and Figure 10 and Figure 11 is respectively magnetic core 2 and epitheca
3 separate after schematic diagram, from figure 10, it is seen that magnetic core 2 have holding tank 23, magnet, holding tank 23 are installed in the holding tank
On be provided with magnetic field transmissive window, magnet is made up of the soft magnetic materials 22 that permanent magnet 21 and its surface are wrapped up, and permanent magnet 21 faces magnetic
The one side of transmissive window is not wrapped, and magnetic field faces out from this and sends into fluid sample, permanent magnet and soft magnetic materials
This combination to greatest extent can be all deflected to magnetic field transmissive window without loss the magnetic field of magnet script increased radioactivity
Magnetic field intensity.From Figure 11 and Figure 12, the similar capsule shape of profile of epitheca 3, but it corresponds to magnetic field transmissive window
Face and its opposite are plane, and observation window 4 is provided with two planes, are glass material, wherein corresponding to magnetic field transmission
The observation window 4 of window is capture observation window, for concentrating capture target particle containing magnetic, the observation window 4 of another side to be used to be carried for observation
For printing opacity, in order to downstream analysis in direct observation.
Embodiment 4
Present embodiments provide a kind of method for sorting specific rare cell with above-mentioned disposable de- magnetic sorting apparatus, including step
Suddenly：
1) immunomagnetic beads, combining target rare cell are added in pretreated blood sample；
2) by the magnetic sorting apparatus above-mentioned blood sample of insertion with epitheca；
3) the whole magnetic sorting apparatus are driven by inserted link, the magnetic core slowly and is gently rotated in the sample
(including revolution and rotation) and move up and down fully to adsorb the target rare cell for being combined with immunomagnetic beads；
4) will adsorb complete magnetic sorting apparatus take out, first insert buffer solution in, by slowly and gently rotate and on
Unnecessary impurity is cleaned in lower movement, is inserted into cell dyeing liquid, by slowly and gently rotate and move up and down come
Ensure fully dyeing；
5) in the magnetic sorting apparatus immigration subsequent cell counting for completing dyeing or the device of analysis, by the observation on epitheca
The counting of window alignment or the light path of analytical equipment, extract inserted link out and only leave epitheca for counting or analyzing and researching.
Preferred embodiment of the invention described in detail above.It should be appreciated that one of ordinary skill in the art without
Need creative work just can make many modifications and variations with design of the invention.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical scheme, all should be in the protection domain being defined in the patent claims.
Claims (10)
1. a kind of disposable de- magnetic sorting apparatus, it is characterised in that including magnetic core and the capture observation window being arranged at outside magnetic core, institute
Capture observation window is stated to be made up of magnetic-permeable material, and the capture observation window is unique regional transmission in the magnetic field that magnetic core sends, by
The magnetic field that magnetic core sends is sent out by capturing observation window, for the target particle containing magnetic in adsorptive liquid sample and described
Capture observation window is detachable.
2. disposable de- magnetic sorting apparatus as claimed in claim 1, it is characterised in that the capture observation window is plane.
3. disposable de- magnetic sorting apparatus as claimed in claim 1, it is characterised in that the capture observation window is by transparent material system
Into.
4. disposable de- magnetic sorting apparatus as claimed in claim 1, it is characterised in that also including inserted link, the magnetic core is arranged at
One end of the inserted link, the inserted link is used for taking and pulling and pushing for the magnetic core.
5. disposable de- magnetic sorting apparatus as claimed in claim 1, it is characterised in that the disposable de- magnetic sorting apparatus also include
Epitheca, the epitheca is removably sheathed on the outside of the magnetic core, and the capture observation window is arranged on the epitheca.
6. disposable de- magnetic sorting apparatus as claimed in claim 1, it is characterised in that be provided with the magnetic core and produce magnetic field
Magnet, the magnet is made up of permanent-magnet material or permanent magnetism with soft magnetism combined material.
7. disposable de- magnetic sorting apparatus as claimed in claim 6, it is characterised in that the magnetic core has capture plane, described to catch
Plane is obtained corresponding to the capture observation window, the magnetic field that the magnet is produced is sent by the capture plane, through the capture
Observation window is acted in fluid sample.
8. disposable de- magnetic sorting apparatus as claimed in claim 7, it is characterised in that the magnet is by permanent magnet and soft magnetic materials
Composition, the permanent magnet is hexahedron, wherein five faces are wrapped up by soft magnetic materials, only expose one it is corresponding with the capture plane
Surface.
9. a kind of method for separating of disposable de- magnetic sorting apparatus as claimed in claim 5, it is characterised in that including step：
1) by the disposable de- magnetic sorting apparatus insertion containing in fluid sample of the target containing magnetic particle；
2) magnetic core is rotated in the sample and/or is moved up and down and carry out abundant adsorbed target particle containing magnetic, so as to complete to mesh
Sorting of the mark containing magnetic particle；
3) the described disposable de- magnetic sorting apparatus for completing will be adsorbed to take out and carry out necessary cleaning, dyeing；
4) will dye in the device of the described disposable de- magnetic sorting apparatus immigration follow-up study for completing, extract the magnetic core out, leave
Counting or observational study of the epitheca for next step.
10. application of the disposable de- magnetic sorting apparatus any one of claim 1-8 in rare cell capture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710155065.7A CN106754352B (en) | 2017-03-14 | 2017-03-14 | The disposable de- magnetic sorting apparatus of one kind and its method for separating |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710155065.7A CN106754352B (en) | 2017-03-14 | 2017-03-14 | The disposable de- magnetic sorting apparatus of one kind and its method for separating |
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| CN106754352A true CN106754352A (en) | 2017-05-31 |
| CN106754352B CN106754352B (en) | 2019-08-02 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111804427A (en) * | 2020-07-24 | 2020-10-23 | 深圳沃德生命科技有限公司 | Collection structure for magnetic particles |
| CN113514190A (en) * | 2021-07-14 | 2021-10-19 | 宁波布拉沃冲气具制造有限公司 | Anti-falling barometer |
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| US5837144A (en) * | 1994-06-16 | 1998-11-17 | Boehringer Mannheim Gmbh | Method of magnetically separating liquid components |
| CN202621280U (en) * | 2012-06-28 | 2012-12-26 | 武汉钢铁(集团)公司 | Rod-type magnetic bar |
| CN203855573U (en) * | 2014-05-28 | 2014-10-01 | 立圜仪器有限公司 | Magnetic rod structure of magnetic bead extraction nucleic acid detection system |
| CN203890368U (en) * | 2013-12-17 | 2014-10-22 | 云南汇博科技有限公司 | Magnetic probe searching type cell sorting device |
| CN205420433U (en) * | 2016-03-14 | 2016-08-03 | 郭亮甫 | Magnetic adsorption shifts stick |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5837144A (en) * | 1994-06-16 | 1998-11-17 | Boehringer Mannheim Gmbh | Method of magnetically separating liquid components |
| CN202621280U (en) * | 2012-06-28 | 2012-12-26 | 武汉钢铁(集团)公司 | Rod-type magnetic bar |
| CN203890368U (en) * | 2013-12-17 | 2014-10-22 | 云南汇博科技有限公司 | Magnetic probe searching type cell sorting device |
| CN203855573U (en) * | 2014-05-28 | 2014-10-01 | 立圜仪器有限公司 | Magnetic rod structure of magnetic bead extraction nucleic acid detection system |
| CN205420433U (en) * | 2016-03-14 | 2016-08-03 | 郭亮甫 | Magnetic adsorption shifts stick |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111804427A (en) * | 2020-07-24 | 2020-10-23 | 深圳沃德生命科技有限公司 | Collection structure for magnetic particles |
| CN113514190A (en) * | 2021-07-14 | 2021-10-19 | 宁波布拉沃冲气具制造有限公司 | Anti-falling barometer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106754352B (en) | 2019-08-02 |
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