CN106748776A - The enzymolysis water extraction of dandelion chlorogenic acid - Google Patents

The enzymolysis water extraction of dandelion chlorogenic acid Download PDF

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Publication number
CN106748776A
CN106748776A CN201611076988.5A CN201611076988A CN106748776A CN 106748776 A CN106748776 A CN 106748776A CN 201611076988 A CN201611076988 A CN 201611076988A CN 106748776 A CN106748776 A CN 106748776A
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China
Prior art keywords
dandelion
enzymolysis
dry powder
chlorogenic acid
water extraction
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Pending
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CN201611076988.5A
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Chinese (zh)
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谢松芬
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谢松芬
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Priority to CN201611076988.5A priority Critical patent/CN106748776A/en
Publication of CN106748776A publication Critical patent/CN106748776A/en
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    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00Preparing tobacco in the factory
    • A24B3/12Steaming, curing, or flavouring tobacco
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00Preparation of carboxylic acid esters
    • C07C67/48Separation; Purification; Stabilisation; Use of additives
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00Preparation of carboxylic acid esters
    • C07C67/48Separation; Purification; Stabilisation; Use of additives
    • C07C67/62Use of additives, e.g. for stabilisation

Abstract

The present invention provides the enzymolysis water extraction of dandelion chlorogenic acid, comprises the following steps:(1) dandelion dry powder is placed in flask, adds deionized water dissolving;(2) enzyme liquid is added to dandelion dry powder solution, 3~5h is digested at a temperature of being placed in 43~51 DEG C;(3) after enzymolysis terminates, after being warming up to 100 DEG C of backflow 2h, after adding chitosan solution to stand 2h, carry out suction filtration concentration and can obtain product.The present invention changes existing single water extraction or alcohol extracting method using water extraction is digested, and is 1 in enzyme liquid addition:4g/ml, solid-liquid ratio are 1:Under the optimised process extraction conditions that 10g/ml, hydrolysis temperature are 47 DEG C, enzymolysis time is 4h, the recovery rate of dandelion Content of Chlorogenic Acid is up to 5.95mg/g, and the extract is applied to make its lifting quality in cigarette, so that dandelion maximum benefit, the method is simple, easily operated, is adapted to industrialized production.

Description

The enzymolysis water extraction of dandelion chlorogenic acid
Technical field
The present invention relates to the extracting method of natural goods, and in particular to a kind of enzymolysis water extraction of dandelion chlorogenic acid.
Background technology
Dandelion, alias dandelion, Po Poding, Hua Hualang etc..Composite family, belongs to herbaceos perennial.Dandelion plant Contain various health-nutrition compositions such as taraxol, taraxacin, choline, organic acid, synanthrin in body.Nature and flavor are sweet, slight bitter, tremble with fear. Return liver, stomach.The effects such as having diuresis, laxative, removing jaundice subcutaneous ulcer, cholagogic.Control heat toxin, carbuncle swells, sore, internal carbuncle, red eye, swell pain, it is damp and hot, The puckery pain of jaundice, dribbling urination, furuncle swelling toxin, acute mastitis, scrofula, toothache, hot eyes, pharyngalgia, lung carbuncle, acute appendicitis, jaundice with damp-heat pathogen, heat gonorrhea are puckery Bitterly.Acute mastitis is controlled, adenolymphitis, scrofula, malignant boil carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis is acute Bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections etc..Dandelion is raw-eaten, stir-fry and eat, cook soup, is the plant of dietotherapeutic.
Containing various active compositions such as substantial amounts of polysaccharide, organic acids in dandelion extract, chlorogenic acid therein is A kind of polyphenols, with important physiologically active.Experiment proves, organic acid (based on chlorogenic acid) in dandelion can be with Flue gas excitant is substantially reduced, aroma quality is improved, so dandelion extract is often used as tobacco aromaticss and is applied in cigarette. At present, the domestic extraction research for dandelion is more, but extraction means are very single, mainly water extraction, alcohol extracting method, recovery rate Low, the utilization rate of dandelion is low, so that the economic worth of dandelion is low.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide the enzymolysis water extraction of dandelion chlorogenic acid, we , using enzymolysis water extraction, extracting factor is good, and recovery rate is high for method, effectively improves the utilization rate of dandelion, lifting dandelion Economic worth.
In order to achieve the above object, the present invention is adopted the technical scheme that:
The enzymolysis water extraction of dandelion chlorogenic acid, comprises the following steps:
(1) dandelion dry powder is placed in clean flask, is 1 by solid-liquid ratio:8~1:12g/ml adds deionization water-soluble Agent is dissolved into dandelion dry powder solution, standby;
(2) solid-liquid ratio 1 of enzyme liquid, the addition enzyme liquid and dandelion dry powder is added to dandelion dry powder solution:3~1: 5g/ml, pH keep nature, are placed in 3~5h of enzymolysis in 43~51 DEG C of thermostat water bath;
(3) after enzymolysis terminates, after the temperature of thermostat water bath is increased into 100 DEG C of backflow 2h, adds and done with dandelion The solid-liquid ratio of powder is 1:3g/ml, concentration are 10g/L chitosan solutions, after standing 2h, carry out suction filtration concentration and can obtain product.
As optimal technical scheme, in order that dandelion Content of Chlorogenic Acid is dissolved by enzyme liquid as far as possible, be conducive to improving green original The recovery rate of acid, improves the utilization rate of dandelion, lifts addition enzyme liquid and Pu in the economic worth of dandelion, the step (2) The solid-liquid ratio 1 of public English dry powder:4g/ml.
As optimal technical scheme, in order that dandelion dry powder dissolves as far as possible, be conducive to improving the recovery rate of chlorogenic acid, The utilization rate of dandelion is improved, the economic worth of dandelion is lifted, while ensureing the consumption of deionized water, cost is effectively controlled, To pressing solid-liquid ratio in dandelion dry powder for 1 in the step (1):10g/ml adds deionized water solvent.
As optimal technical scheme, in order that dandelion Content of Chlorogenic Acid is dissolved by enzyme liquid as far as possible, be conducive to improving green original The recovery rate of acid, improves the utilization rate of dandelion, lifts the economic worth of dandelion, and 47 DEG C of temperature is placed in the step (2) The lower enzymolysis of degree.
As optimal technical scheme, in order that dandelion Content of Chlorogenic Acid is dissolved by enzyme liquid as far as possible, be conducive to improving green original The recovery rate of acid, improves the utilization rate of dandelion, lifts enzymolysis 4h in the economic worth of dandelion, the step (2).
Compared with prior art, the benefit that the present invention has:The present invention changes existing single water using water extraction is digested Carry or alcohol extracting method, be 1 in enzyme liquid addition:4g/ml, solid-liquid ratio are 1:10g/ml, hydrolysis temperature are 47 DEG C, enzymolysis time is 4h Optimised process extraction conditions under, the recovery rate of dandelion Content of Chlorogenic Acid is up to 5.95mg/g, and the extract is applied to cigarette In make its sweet sense of lifting, excitant, pleasant impression, dry sensation, while make to lose smaller on its fragrance level, so that dandelion is most Golden eggs, the method is simple, easily operated, extraction cost is low, is adapted to industrialized production.
Specific embodiment
The present invention is further described with reference to specific embodiment.
Embodiment 1
The enzymolysis water extraction of dandelion chlorogenic acid, comprises the following steps:
(1) dandelion dry powder is placed in clean flask, is 1 by solid-liquid ratio:8g/ml adds deionized water solvent dissolving It is standby into dandelion dry powder solution;
(2) solid-liquid ratio 1 of enzyme liquid, the addition enzyme liquid and dandelion dry powder is added to dandelion dry powder solution:3g/ml, PH keeps nature, is placed in 43 DEG C of thermostat water bath and digests 3h;
(3) after enzymolysis terminates, after the temperature of thermostat water bath is increased into 100 DEG C of backflow 2h, adds and done with dandelion The solid-liquid ratio of powder is 1:3g/ml, concentration are 10g/L chitosan solutions, after standing 2h, carry out suction filtration concentration and can obtain product.
Embodiment 2
The enzymolysis water extraction of dandelion chlorogenic acid, comprises the following steps:
(1) dandelion dry powder is placed in clean flask, is 1 by solid-liquid ratio:9g/ml adds deionized water solvent dissolving It is standby into dandelion dry powder solution;
(2) solid-liquid ratio 1 of enzyme liquid, the addition enzyme liquid and dandelion dry powder is added to dandelion dry powder solution:3.5g/ Ml, pH keep nature, are placed in 45 DEG C of thermostat water bath and digest 3.5h;
(3) after enzymolysis terminates, after the temperature of thermostat water bath is increased into 100 DEG C of backflow 2h, adds and done with dandelion The solid-liquid ratio of powder is 1:3g/ml, concentration are 10g/L chitosan solutions, after standing 2h, carry out suction filtration concentration and can obtain product.
Embodiment 3
The enzymolysis water extraction of dandelion chlorogenic acid, comprises the following steps:
(1) dandelion dry powder is placed in clean flask, is 1 by solid-liquid ratio:10g/ml adds deionized water solvent molten Solution is standby into dandelion dry powder solution;
(2) solid-liquid ratio 1 of enzyme liquid, the addition enzyme liquid and dandelion dry powder is added to dandelion dry powder solution:4g/ml, PH keeps nature, is placed in 47 DEG C of thermostat water bath and digests 4h;
(3) after enzymolysis terminates, after the temperature of thermostat water bath is increased into 100 DEG C of backflow 2h, adds and done with dandelion The solid-liquid ratio of powder is 1:3g/ml, concentration are 10g/L chitosan solutions, after standing 2h, carry out suction filtration concentration and can obtain product.
Embodiment 4
The enzymolysis water extraction of dandelion chlorogenic acid, comprises the following steps:
(1) dandelion dry powder is placed in clean flask, is 1 by solid-liquid ratio:11g/ml adds deionized water solvent molten Solution is standby into dandelion dry powder solution;
(2) solid-liquid ratio 1 of enzyme liquid, the addition enzyme liquid and dandelion dry powder is added to dandelion dry powder solution:4.5g/ Ml, pH keep nature, are placed in 49 DEG C of thermostat water bath and digest 4.5h;
(3) after enzymolysis terminates, after the temperature of thermostat water bath is increased into 100 DEG C of backflow 2h, adds and done with dandelion The solid-liquid ratio of powder is 1:3g/ml, concentration are 10g/L chitosan solutions, after standing 2h, carry out suction filtration concentration and can obtain product.
Embodiment 5
The enzymolysis water extraction of dandelion chlorogenic acid, comprises the following steps:
(1) dandelion dry powder is placed in clean flask, is 1 by solid-liquid ratio:12g/ml adds deionized water solvent molten Solution is standby into dandelion dry powder solution;
(2) solid-liquid ratio 1 of enzyme liquid, the addition enzyme liquid and dandelion dry powder is added to dandelion dry powder solution:5g/ml, PH keeps nature, is placed in 51 DEG C of thermostat water bath and digests 5h;
(3) after enzymolysis terminates, after the temperature of thermostat water bath is increased into 100 DEG C of backflow 2h, adds and done with dandelion The solid-liquid ratio of powder is 1:3g/ml, concentration are 10g/L chitosan solutions, after standing 2h, carry out suction filtration concentration and can obtain product.
It should be pointed out that above case is only representational example of the invention.Obviously, technical scheme is not limited In above-described embodiment, there can also be many denaturation.

Claims (5)

1. the enzymolysis water extraction of dandelion chlorogenic acid, it is characterised in that comprise the following steps:
(1) dandelion dry powder is placed in clean flask, is 1 by solid-liquid ratio:8~1:12g/ml adds deionized water solvent molten Solution is standby into dandelion dry powder solution;
(2) solid-liquid ratio 1 of enzyme liquid, the addition enzyme liquid and dandelion dry powder is added to dandelion dry powder solution:3~1:5g/ml, PH keeps nature, is placed in 3~5h of enzymolysis in 43~51 DEG C of thermostat water bath;
(3) after enzymolysis terminates, after the temperature of thermostat water bath is increased into 100 DEG C of backflow 2h, add and dandelion dry powder Solid-liquid ratio is 1:3g/ml, concentration are 10g/L chitosan solutions, after standing 2h, carry out suction filtration concentration and can obtain product.
2. the enzymolysis water extraction of dandelion chlorogenic acid according to claim 1, it is characterised in that:In the step (2) Add the solid-liquid ratio 1 of enzyme liquid and dandelion dry powder:4g/ml.
3. the enzymolysis water extraction of dandelion chlorogenic acid according to claim 2, it is characterised in that:In the step (1) It is 1 to solid-liquid ratio is pressed in dandelion dry powder:10g/ml adds deionized water solvent.
4. the enzymolysis water extraction of dandelion chlorogenic acid according to claim 3, it is characterised in that:In the step (2) Digested at a temperature of being placed in 47 DEG C.
5. the enzymolysis water extraction of dandelion chlorogenic acid according to claim 4, it is characterised in that:In the step (2) Enzymolysis 4h.
CN201611076988.5A 2016-11-30 2016-11-30 The enzymolysis water extraction of dandelion chlorogenic acid Pending CN106748776A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109619642A (en) * 2018-11-14 2019-04-16 河南中烟工业有限责任公司 A kind of fragrance-enhancing tobacco tobacco extract and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101486651A (en) * 2009-02-10 2009-07-22 江苏省苏微微生物研究有限公司 Method for extracting chlorogenic acid from Eucommia leaves and use thereof
CN101851163A (en) * 2010-06-10 2010-10-06 桂阳济草堂药材种业有限公司 Method for extracting high-purity chlorogenic acid from honey suckle flower
CN103333067A (en) * 2013-06-13 2013-10-02 广西金昊生物科技有限公司 Extraction method of high-purity chlorogenic acid

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101486651A (en) * 2009-02-10 2009-07-22 江苏省苏微微生物研究有限公司 Method for extracting chlorogenic acid from Eucommia leaves and use thereof
CN101851163A (en) * 2010-06-10 2010-10-06 桂阳济草堂药材种业有限公司 Method for extracting high-purity chlorogenic acid from honey suckle flower
CN103333067A (en) * 2013-06-13 2013-10-02 广西金昊生物科技有限公司 Extraction method of high-purity chlorogenic acid

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Title
林春梅: "蒲公英中绿原酸纤维素酶法提取工艺的优化", 《湖北农业科学》 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109619642A (en) * 2018-11-14 2019-04-16 河南中烟工业有限责任公司 A kind of fragrance-enhancing tobacco tobacco extract and preparation method thereof

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Application publication date: 20170531