CN106748104A - A kind of preparation method of white ginseng bacterium pedigree seed culture medium - Google Patents
A kind of preparation method of white ginseng bacterium pedigree seed culture medium Download PDFInfo
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- CN106748104A CN106748104A CN201611157706.4A CN201611157706A CN106748104A CN 106748104 A CN106748104 A CN 106748104A CN 201611157706 A CN201611157706 A CN 201611157706A CN 106748104 A CN106748104 A CN 106748104A
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- 241000208340 Araliaceae Species 0.000 title claims abstract description 33
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 33
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 33
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 33
- 241000894006 Bacteria Species 0.000 title claims abstract description 32
- 239000001963 growth medium Substances 0.000 title claims abstract description 16
- 238000011218 seed culture Methods 0.000 title claims abstract description 10
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
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- 150000001875 compounds Chemical class 0.000 claims abstract description 6
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- 229920000573 polyethylene Polymers 0.000 claims abstract description 4
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- 238000012360 testing method Methods 0.000 claims abstract description 4
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- 238000009413 insulation Methods 0.000 claims description 2
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000011161 development Methods 0.000 description 3
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- 235000013305 food Nutrition 0.000 description 3
- 241000894007 species Species 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000446313 Lamella Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 241000222481 Schizophyllum commune Species 0.000 description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 229910052732 germanium Inorganic materials 0.000 description 2
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 2
- LEQAOMBKQFMDFZ-UHFFFAOYSA-N glyoxal Chemical compound O=CC=O LEQAOMBKQFMDFZ-UHFFFAOYSA-N 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000001630 malic acid Substances 0.000 description 2
- 229940099690 malic acid Drugs 0.000 description 2
- 235000011090 malic acid Nutrition 0.000 description 2
- 210000002686 mushroom body Anatomy 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052711 selenium Inorganic materials 0.000 description 2
- 239000011669 selenium Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- 241000609240 Ambelania acida Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
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- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 241000410367 Clerodendrum thomsoniae Species 0.000 description 1
- 241001232787 Epiphragma Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
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- 240000007594 Oryza sativa Species 0.000 description 1
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- 208000009205 Tinnitus Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
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- 229910021529 ammonia Inorganic materials 0.000 description 1
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- 230000001684 chronic effect Effects 0.000 description 1
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- 230000007123 defense Effects 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
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- 230000007613 environmental effect Effects 0.000 description 1
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- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- GVALZJMUIHGIMD-UHFFFAOYSA-H magnesium phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O GVALZJMUIHGIMD-UHFFFAOYSA-H 0.000 description 1
- 239000004137 magnesium phosphate Substances 0.000 description 1
- 229910000157 magnesium phosphate Inorganic materials 0.000 description 1
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- 235000010994 magnesium phosphates Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
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- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of preparation method of white ginseng bacterium pedigree seed culture medium, agricultural technology field, it is characterized in that being carried out by following step:First, raw material prepares:Cotton seed hulls 38%, corncob 30%, wheat bran 18%, corn flour 2%, weed tree sawdust 10%, land plaster 1%, calcium magnesium phosphate 1%;2nd, being well mixed after coarse fodder is crushed turns into mixed culture material, and mixed culture material is distributed into polypropylene film bag;3rd, culture medium goes out miscellaneous bacteria:Put sacked material heap into sterilization tank, put sterilization tank into and be heated to 80 DEG C ~ 100 DEG C;4th, white ginseng original seed mycelia is taken out from test tube, gripping fritter puts compound into surface, and benefit is filled out 1.0 cm of thickness ~ 1.5cm, slightly flattened with flat board, and uniform dispersing dry defecates water to just humidity;6th, overlay film moisture-heat preservation:A black polyethylene film is covered above culture bag, 25 DEG C ~ 30 DEG C of canopy temperature is kept, there is thalline discharge feed side long after 7 ~ 8 days, at this moment withdraw agricultural film.
Description
Technical field
The present invention relates to a kind of preparation method of white ginseng bacterium pedigree seed culture medium, belong to agricultural technology field.
Background technology
White ginseng bacterium, alias:Schizophyllum commune, spend in vain, white ginseng, section category:Mycophyta door fungi schizophyllum commune
(schizophyllqaeae)A kind of food medicine dual-purpose rare mushroom.Its cap is wide 0.6~4.2 centimetre, and matter is tough, and white is extremely
Canescence, upper villous, fan-shaped or kidney shape has many rip-panels.Lamella is narrow, is radiated from base portion and gone out, and handle is short or nothing.Mushroom constitution is tough,
Fragrant taste, deliciousness are tasty and refreshing, nutritious.
The white ginseng bacterium of artificial cultivation is analyzed according to test center of academy of agricultural sciences of Yunnan Province, 8 kinds of ammonia needed by human in white ginseng bacterium
Base acid total content and rich in zinc, iron, potassium, calcium, phosphorus, selenium, germanium, and has medical value higher up to 17.04%.According to《It is medicinal true
Bacterium》Recorded Deng nationality, this bacterium " mild-natured, sweet, smell(Root)It is bitter, be slightly cold, it is nontoxic.Clear liver and improve vision, nourishing and fit keeping function, it is special
It is other obvious to disease curative effects such as infantile night sweat, gynecological disease, neurasthenia, dizzy tinnitus." from white ginseng bacterium extract polysaccharide
(SPG), mononuclear phagocyte system activity can be stimulated, there is significant defense effectiveness to chronic bacterial infection, there is suppression to tumour
Effect;The organic acid of its mycelia submerged fermentation output, is widely used in the aspects such as food, medical industry and biochemistry.Containing zinc,
Iron, potassium, calcium, phosphorus, selenium, germanium, and have medical value higher.
White ginseng mushroom entity is smaller, fasciation or all living creatures, is similar to chrysanthemum, and cap is fan-shaped or kidney shape, 1~5cm of diameter, Planting
Single 50~100g that weighs of fresh goods of training.Bacterial context is thin, and matter is tough, white to canescence or brown yellowish pink.With most rip-panels, edge is normal
Involute, the surface villous long in palm shape lobe;Base portion is narrow, and, from the radial growth of base portion, stem is short or nothing for lamella.
The ability that white ginseng bacterium decomposes timber is weaker, and carbon and nitrogen sources are its important energy.Suitable carbon source is glucose, nitrogen
Source is corn flour, wheat bran.Artificial cultivation can be using various farmings rich in cellulose such as cotton seed hulls, corncob, bagasse, waste cottons
Thing stalk and wood chip etc. are used as compost;Also need to add the auxiliary materials such as appropriate wheat bran or rice bran and trace mineral element.
The Pseudomonas grows many in spring, autumn naturally in middle warm type mushroom.Artificial domesticating cultivation shows, mycelial growth temperature
Degree wider range, 8~32 DEG C, but it is most suitable with 23~26 DEG C;Fruiting body differentiation and development are most suitable with 18~22 DEG C, less than 18
The extension of DEG C maturity period.
White ginseng bacterium is drought-resistant.Moisture content in medium is no more than 60%.Fructification dry and wet retractility is big, and cell is swollen when having moisture
Greatly, shunk when drying, continue to stretch again after moisture is suctioned.Mushroom stage space relative humidity long should rest in 85%~90%.
Mycelial growth is not required to illumination, it is necessary to 300~500Lx intensities of illumination during former base differentiation fructification.Fructification has the light that significantly becomes
Property, light is too strong to make mushroom body color browning, poor quality.The bacterium cellulase, and malic acid can be produced(1-
malicacid.C2H2O2).The substrate pH 4.5~5.6 of artificial cultivation is most suitable.
The cultivation of general white ginseng bacterium, when bacterium bag enters canopy restocking swing bag flower bud, distinguishes different pouch-shaped operations:Dialling for short bag is gone
Sack tampon, stretches a bag film fruiting;Also bag wall surrounding can be taken every 8cm, the mushroom long mouthful of 1~2cm is drawn with blade, then by bacterium
Bag is erect and is put, many mouthfuls of fruitings.Bag long enter canopy after, it is horizontally-arranged after environment 2 days is adapted in rack-layer, the adhesive plaster in pocket is torn
Fall, the upward mushroom long of pocket.Then in space spray form water, relative air humidity is maintained at 80%, and covers rack-layer cover film moisturizing.
Daily during water spray, note taking off film ventilation.Formed to former base behind bacterium bag opening and generally required 4~6 days.Taken off when mushroom flower bud slightly breaks up
Remove epiphragma.It is early, middle and late daily to space spray form water 1 time, on unsuitable direct-injection mushroom body, air phase as fruit body development needs
85%~90% is kept to humidity;Temperature requirement is not less than 18 DEG C, no more than 25 DEG C;300~500Lx scattering lights are given, is promoted
Fructification normal development.cid.C2H2O2).The substrate pH 4.5~5.6 of artificial cultivation is most suitable.
White ginseng bacterium needs 16~20 days, when fructification blade is open and flat under suitable environmental condition from harvesting is seeded to
Can harvest.Cut from base portion with scraper during harvesting, and repair net base portion impurity.Stop water spray before harvesting, it is to avoid brittle failure damages a shape.
Stop water spray 2 days after general 1st damp mushroom harvesting, live bacteria, then manages as usual;After 7 days again the damp mushroom of output the 2nd.Manage
2~3 damp mushrooms, biological transformation ratio 40%~50% can be typically grown at that time.200~the 250g of per unit area yield of bag long, the per unit area yield 100 of short bag~
150g.Fresh goods foam plastic magazine and preservative film packaging are sent to supermarket, under 4~5 DEG C of low temperature, shelf life 12~14 days;Processing
When drying, can be dried with mechanical dehydration, fresh dry product ratio is 4: 1, and dry product is moistureproof with double-layer plastic bag hermetic package.
The content of the invention
White ginseng bacterium pedigree seed culture medium, is to extract the parent species that portion of tissue is cultivated in white ginseng mushroom entity bacterial strain, by parent species
White ginseng bacterium original seed is further trained, white ginseng bacterium original seed is trained white ginseng mushroom entity bacterial strain on culture medium again.
A kind of preparation method solve problem of white ginseng bacterium pedigree seed culture medium is adopted the technical scheme that by following step
Carry out:First, raw material prepares:Cotton seed hulls 38%, corncob 30%, wheat bran 18%, corn flour 2%, weed tree sawdust 10%, land plaster 1%, calcium and magnesium
Phosphate fertilizer 1%;2nd, coarse fodder is crushed:Cotton seed hulls, corncob, weed tree sawdust are ground into the chip of diameter≤0.2cm, be blended into wheat bran,
Corn flour, land plaster, calcium magnesium phosphate, being well mixed turns into mixed culture material, and mixed culture material is distributed into polypropylene film bag,
Every bag of 0.3kg ~ 0.4 kg;3rd, culture medium goes out miscellaneous bacteria:Put sacked material heap into sterilization tank, mixed stacking makes to be flowed between bag and bag
Logical hot gas, sterilization tank is heated to 80 DEG C ~ 100 DEG C, the atmospheric pressure of pressure 2 ~ 2.5, and temperature to 20 is reduced naturally after being kept for 65 ~ 80 minutes
DEG C ~ 30 DEG C, case-opening goods-taking goes out Bag Material;4th, white ginseng original seed mycelia is taken out from test tube, grips fritter, will first cultivate the mixed of sack
Close material and take out 100g ~ 200g, then put fritter white ginseng original seed mycelia into, then mend the compound for filling out taking-up in surface, benefit fills out thickness
1.0 cm ~ 1.5cm, are slightly flattened with flat board, and sack upward stop and be put on greenhouse floor by row pattern, and uniform dispersing dry defecates water extremely
It is just moist;6th, overlay film moisture-heat preservation:A black polyethylene film is covered above culture bag, canopy temperature 25 DEG C ~ 30 is kept
DEG C, there is thalline discharge feed side long after 7 ~ 8 days, at this moment withdraw agricultural film.
A kind of beneficial effect of the preparation method of white ginseng bacterium pedigree seed culture medium is:A kind of making of white ginseng bacterium pedigree seed culture medium
Method, as raw material, is equipped with gypsum and appropriate chemistry fertilizer using the various plants fibers such as cotton seed hulls, corncob and energy matter
Material, by crushing, mixing, and is processed by high temperature and high pressure sterilization, has eliminated miscellaneous bacteria present in culture medium;Pass through again
By the seed covering of white ginseng bacterial initial species mycelia, white ginseng mushroom entity can be successfully turned out, meet the abundant food demand of people.
Specific embodiment
A kind of preparation method of white ginseng bacterium pedigree seed culture medium, is specifically carried out by following step:First, raw material prepares:Cotton
Seed shell 38%, corncob 30%, wheat bran 18%, corn flour 2%, weed tree sawdust 10%, land plaster 1%, calcium magnesium phosphate 1%;2nd, coarse fodder is crushed:
Cotton seed hulls, corncob, weed tree sawdust are ground into the chip of diameter≤0.2cm, wheat bran, corn flour, land plaster, calcium and magnesium phosphorus is blended into
Fertilizer, being well mixed turns into mixed culture material, and mixed culture material is distributed into polypropylene film bag, every bag of 0.3kg ~ 0.4 kg;3rd,
Culture medium goes out miscellaneous bacteria:Put sacked material heap into sterilization tank, mixed stacking is heated to the hot gas that can be circulated between bag and bag, sterilization tank
80 DEG C ~ 100 DEG C, the atmospheric pressure of pressure 2 ~ 2.5, temperature to 20 DEG C ~ 30 DEG C, case-opening goods-taking output are reduced after being kept for 65 ~ 80 minutes naturally
Material;4th, white ginseng original seed mycelia is taken out from test tube, grips fritter, the compound that will first cultivate sack takes out 100g ~ 200g,
Then put fritter white ginseng original seed mycelia into, then mend and fill out the compound of taking-up in surface, benefit fills out 1.0 cm of thickness ~ 1.5cm, with flat
Plate is slightly flattened, and sack upward stop and be put on greenhouse floor by row pattern, and uniform dispersing dry defecates water to just moist;6th, overlay film is protected
Wet insulation:A black polyethylene film is covered above culture bag, 25 DEG C ~ 30 DEG C of canopy temperature is kept, has thalline long after 7 ~ 8 days
Discharge feed side, at this moment withdraws agricultural film.
Claims (1)
1. a kind of preparation method of white ginseng bacterium pedigree seed culture medium, it is characterized in that being carried out by following step:First, raw material prepares:Cotton
Seed shell 38%, corncob 30%, wheat bran 18%, corn flour 2%, weed tree sawdust 10%, land plaster 1%, calcium magnesium phosphate 1%;2nd, coarse fodder is crushed:
Cotton seed hulls, corncob, weed tree sawdust are ground into the chip of diameter≤0.2cm, wheat bran, corn flour, land plaster, calcium and magnesium phosphorus is blended into
Fertilizer, being well mixed turns into mixed culture material, and mixed culture material is distributed into polypropylene film bag, every bag of 0.3kg ~ 0.4 kg;3rd,
Culture medium goes out miscellaneous bacteria:Put sacked material heap into sterilization tank, mixed stacking is heated to the hot gas that can be circulated between bag and bag, sterilization tank
80 DEG C ~ 100 DEG C, the atmospheric pressure of pressure 2 ~ 2.5, temperature to 20 DEG C ~ 30 DEG C, case-opening goods-taking output are reduced after being kept for 65 ~ 80 minutes naturally
Material;4th, white ginseng original seed mycelia is taken out from test tube, grips fritter, the compound that will first cultivate sack takes out 100g ~ 200g,
Then put fritter white ginseng original seed mycelia into, then mend and fill out the compound of taking-up in surface, benefit fills out 1.0 cm of thickness ~ 1.5cm, with flat
Plate is slightly flattened, and sack upward stop and be put on greenhouse floor by row pattern, and uniform dispersing dry defecates water to just moist;6th, overlay film is protected
Wet insulation:A black polyethylene film is covered above culture bag, 25 DEG C ~ 30 DEG C of canopy temperature is kept, has thalline long after 7 ~ 8 days
Discharge feed side, at this moment withdraws agricultural film.
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CN107828664A (en) * | 2017-10-25 | 2018-03-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT 1 and its cultural method and application |
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CN107828664A (en) * | 2017-10-25 | 2018-03-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT 1 and its cultural method and application |
CN107828664B (en) * | 2017-10-25 | 2020-10-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT-1 and cultivation method and application thereof |
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