CN106728762A - It is a kind of to treat Chinese medicine composition of cervical carcinoma and preparation method thereof - Google Patents
It is a kind of to treat Chinese medicine composition of cervical carcinoma and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/748—Oldenlandia or Hedyotis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/648—Myriapods, e.g. centipedes or millipedes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/78—Saururaceae (Lizard's-tail family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Abstract
The invention belongs to technical field of traditional Chinese medicines, it is specifically related to a kind of to treat Chinese medicine composition of cervical carcinoma and preparation method thereof, it is made as raw material with oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia, Scolopendra extract, by after rational compatibility, required formulation is made, the medicine is evident in efficacy, have no drug resistance, recurrence rate is low, toxicity is low, and can effectively suppress the transfer of tumour cell, promote apoptosis of tumor cells, improve anti-cancer ability.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, and in particular to a kind of Chinese medicine composition for treating cervical carcinoma and its preparation side
Method.
Background technology
Cervical carcinoma is to belong to the traditional Chinese medical science " uterine bleeding ", " coloured vaginal discharge ", the category of " lump in the abdomen is gathered ", by the dirty disorder of qi and blood of internal organs, leaching in humidity,
Accumulate product under, damage wantonly two arteries and veins of punching and form, Zhang Shizhong scapes point out that " the empty long-pending cold junction gas of the cause of disease of married woman, is that Zhu Jingshui breaks off, to having
Pathogenic cold coagulating with blood gastral cavity door over the years, cold injury of meridian ", Li Donghuan points out " married woman metrorrhagia person, by internal organs wantonly two arteries and veins of dirty wound punching, all empty events of qi and blood
, two arteries and veins are the sea of vein, and vim and vigour trip follows outward channels and collaterals, and interior flourish internal organs is dirty, if qi and blood is adjusted, under according to when, pole of such as working,
The dirty all wounds of internal organs, the deficiency of vital energy that punching is appointed can not restrict its menses, thus suddenly under, under the metrorrhagia of meaning is sudden and violent ", according to its clinical manifestation, can
It is summarized as the symptoms such as stagnation of QI due to depression of the liver, damp and hot silt poison, the deficiency of liver-yin and kidney-yin, the insufficiency of both the spleen and the kindey, the insufficiency of heart and spleen, the sinking of qi of middle-jiao.
At present, the method for the treatment of cervical carcinoma has a lot, although such as chemotherapy means can reduce gross tumor volume, because of ischemic and
The influence of slough, can aggravate tumor hypoxia, increase tolerance of the tumour to radiotherapy, and Common Chemotherapy medicine 5- fluorine ureas are phonetic
Pyridine, cis-platinum, adriamycin etc. enter row vein or local injection, can produce toxic reaction, there is significant impact to reproductive function, and dynamic
Find that there is carcinogenesis in thing experiment, potential carcinogenic, mutagenesis is there is also in human body；Operation can no doubt cut off cancer
It is swollen, but cancer embolus presence etc. in also residual cancer or regional lymph node metastasis or blood vessel；And treatment by Chinese herbs is taken righting and eliminating evil, is attacked
With mending, taking stopgap measures the measure such as be combined with effecting a permanent cure, controlled the cancer stove of cervical cancer patient, cancerous swelling diminution, symptom mitigation, existence
Phase extension, cure rate improve, and can prevent from recurring and shifting, and can also mitigate the side effect of chemotherapy, but most medicine
Treatment cycle is long, effect is undesirable.
The content of the invention
In order to solve technical problem present in prior art, it is an object of the invention to provide a kind of cervical carcinoma for the treatment of
Chinese medicine composition, the Chinese medicine composition is evident in efficacy, have no drug resistance, recurrence rate is low, and can effectively suppress tumour cell
Transfer, lifts the immunocompetence of body, promotes apoptosis of tumor cells, improves anti-cancer ability.
Raw material is in parts by weight：20~35 parts of oldenlandia diffusa, 20~30 parts of Sculellaria barbata, 22~31 parts of phoenix-tail fern, horse
6~12 parts of part of whip grass, 8~17 parts of garlic, 10~15 parts of cordate houttuynia, 5~10 parts of Scolopendra extract.
More preferably 27 parts of oldenlandia diffusa, 25 parts of Sculellaria barbata, 26 parts of phoenix-tail fern, 7 parts of Verbena officinalis, 10 parts of garlic,
14 parts of cordate houttuynia, 7 parts of Scolopendra extract.
A kind of preparation method of described Chinese medical extract, specific preparation process is as follows：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 150~200 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 42~48 DEG C, extracting pressure
5~7MPa, 2~3h of extraction time, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 4~7%, addition 5g/L solid inorganic salts and 2) in carry
Thing is taken, temperature extraction takes at 60~70 DEG C, extracts 3 times, and each 2~3h of extraction time obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder.
The powder, by addition or without pharmaceutically acceptable carrier, is made different dosage forms.
The solid inorganic salt is one or more in saleratus, sodium iodide, sodium chloride, potassium acetate.
The spray drying, 150~180 DEG C of spray tower air themperature goes out 80~110 DEG C of tower air themperature.
The powder, by addition or without pharmaceutically acceptable carrier, is made different dosage forms.
The Scolopendra extract, its preparation method：Centipede cephalopodium, bamboo stick are removed, crushed after being dried into 300 mesh, with nothing
Water-ethanol degreasing, adds 6 times of Tris-Triton-NaCl buffer solutions, and 8h, high speed centrifugation 5min are oozed in cold at 4 DEG C, takes green
Color supernatant, adds (the NH equivalent to 1.5 times of supernatant liquid measure4)2SO4, 10min is centrifuged again, incline supernatant, after dialysis
Scolopendra extract is obtained.
The buffer solution is any one in Tris-Triton-NaCl buffer solutions, PBS.
Beneficial effect of the present invention：
Oldenlandia diffusa is sweet, light, cool in nature, clearing heat and detoxicating, and dampness removing disappears carbuncle, anticancer, for malignant tumour, acute appendicitis, throat
Swell and ache, jaundice with damp-heat pathogen, difficult urination, sore furuncle poison, venomous snake bite.
It is Sculellaria barbata taste is pungent, flat, have clearing heat and detoxicating, promoting blood circulation and removing blood stasis, swelling and pain relieving, the effect of anticancer；Appendicitis curing, hepatitis,
Stomachache, early liver cancer, lung cancer, cervix cancer, mastitis etc.；The diseases such as the treatment of pain furuncle, treating swelling and pain by traumatic injury are controlled in external application.
Phoenix-tail fern is lightly seasoned, slight bitter, cool in nature, clearing heat and promoting diuresis, detoxify stop dysentery, cooling blood and hemostasis.For dysentery, gastroenteritis, hepatitis,
Urinary infection, cat fever, abscess of throat, leukorrhea, uterine bleeding, pesticide poisoning；Traumatism and bleeding, burn and scald are controlled in external application.
Verbena officinalis taste micro- Wei, it is cool in nature, have functions that cool blood, dissipate the stasis of blood, stimulate the menstrual flow, heat-clearing, removing toxic substances, antipruritic, expelling parasite, dissipate-swelling.
Garlic has the peppery gas of strong garlic, and taste is pungent is peppery, and detoxify desinsection, and swelling and pain relieving, antidiarrheal stop dysentery controls lung, expelling parasite, additionally
There is warm spleen warm stomach；Control swollen ulcer drug, white bald tinea sore, dysentery diarrhea, tuberculosis pertussis, roundworm pinworm, eating accumulation, coldness and pain in the epigastrium,
Oedema turgor.
Cordate houttuynia taste is pungent, cold, return lung warp has clearing heat and detoxicating, detumescence treatment sore, promoting urination and removing dampness, clearing away heat to cure dysentery, stomach invigorating to disappear
Food, with control real heat, heat toxin, damp evil, disease heat be suffer from lung carbuncle, sore swollen toxin, hemorrhoidal hemorrhage, retention of heat in the spleen and stomach the effects such as.
Centipede property of medicine pungent-warm, poisonous, return liver warp can be adjusted up to Liver Channel, with treatment child convulsion, tic spasm, desolation purulence
The effects such as swollen, middle air port wo, rheumatoid arthritis stubborn.
The present invention uses oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia, centipede to be prepared for raw material
Form, raw material uses supercritical extract so that active ingredient is extracted, compared to traditional Chinese medicine preparation mode, can
To reduce the loss of anticancer component, therapeutic effect is improved；Centipede single is used as medicine in the aspect tool such as treatment stomach cancer, cancer of the esophagus, lung cancer
There is good effect, its main anticancer component is anti-tumor protein, and coordinates other traditional Chinese medicine ingredients in the present invention, form collaboration
The effect of anticancer, and be better than centipede single and be used as medicine, the rational proportion of raw material so that the curative effect of medication being made significantly, have no drug resistance,
Recurrence rate is low, and can effectively suppress the transfer of tumour cell, promotes apoptosis of tumor cells, improves anti-cancer ability.
Specific embodiment
Technical scheme is further limited with reference to specific embodiment, but claimed
Scope is not only limited to description.
Embodiment 1
Formula：Oldenlandia diffusa 20g, Sculellaria barbata 20g, phoenix-tail fern 22g, Verbena officinalis 6g, garlic 8g, cordate houttuynia 10g, centipede
Extract 5g；
Preparation method：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 150 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 42~44 DEG C, extracting pressure
5MPa, extraction time 2h, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 4%, addition 5g/L saleratus and 2) in extract,
Temperature extraction takes at 60~62 DEG C, extracts 3 times, and each extraction time 2h obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder, spray tower
150~155 DEG C of air themperature, goes out 80~85 DEG C of tower air themperature, microcrystalline cellulose is added, through compressing tablet, you can tablet is obtained.
Embodiment 2
Formula：Oldenlandia diffusa 35g, Sculellaria barbata 30g, phoenix-tail fern 31g, Verbena officinalis 12g, garlic 17g, cordate houttuynia 15g, Wu
Centipede extract 10g；
Preparation method：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 200 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 46~48 DEG C, extracting pressure
6MPa, extraction time 3h, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 7%, addition 5g/L sodium iodides and 2) in extract,
Temperature extraction takes at 68~70 DEG C, extracts 3 times, and each extraction time 3h obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder, spray tower
175~180 DEG C of air themperature, goes out 105~115 DEG C of tower air themperature, is crushed to 300~400 mesh, adds 2~4 times of starch,
Can be prepared by electuary.
Embodiment 3
Formula：Oldenlandia diffusa 27g, Sculellaria barbata 25g, phoenix-tail fern 26g, Verbena officinalis 7g, garlic 10g, cordate houttuynia 14g, Wu
Centipede extract 7g；
Preparation method：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 170 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 44~46 DEG C, extracting pressure
5.5MPa, extraction time 2.5h, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 6%, addition 5g/L sodium chloride and 2) in extract,
Temperature extraction takes at 65~67 DEG C, extracts 3 times, and each extraction time 3h obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder, spray tower
160~165 DEG C of air themperature, goes out 90~95 DEG C of tower air themperature, adds lactose, is made powder.
Embodiment 4
Formula：Oldenlandia diffusa 30g, Sculellaria barbata 27g, phoenix-tail fern 25g, Verbena officinalis 10g, garlic 12g, cordate houttuynia 13g, Wu
Centipede extract 6g；
Preparation method：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 180 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 46~48 DEG C, extracting pressure
6.5MPa, extraction time 3h, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 5%, addition 5g/L potassium acetates and 2) in extract,
Temperature extraction takes at 63~65 DEG C, extracts 3 times, and each extraction time 2.5h obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder, spray tower
170~175 DEG C of air themperature, goes out 100~150 DEG C of tower air themperature, adds sucrose, is made decoction.
Embodiment 5
Formula：Oldenlandia diffusa 30g, Sculellaria barbata 20g, phoenix-tail fern 22g, Verbena officinalis 6g, garlic 17g, cordate houttuynia 15g, Wu
Centipede extract 9g；
Preparation method：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 160 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 43~45 DEG C, extracting pressure
6MPa, extraction time 3h, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 5.5%, addition 3g/L saleratus, 2g/L sodium iodides
With 2) in extract, at 67~69 DEG C temperature extraction take, extract 3 times, each extraction time 2h obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder, spray tower
165~170 DEG C of air themperature, goes out 95~100 DEG C of tower air themperature, adds starch, is made granule.
Embodiment 6
Formula：Oldenlandia diffusa 20g, Sculellaria barbata 30g, phoenix-tail fern 31g, Verbena officinalis 12g, garlic 8g, cordate houttuynia 10g, Wu
Centipede extract 8g；
Preparation method：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 190 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 45~47 DEG C, extracting pressure
7MPa, extraction time 3h, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 6.5%, addition 2.5g/L sodium chloride, 2.5g/L acetic acid
Potassium and 2) in extract, at 65~67 DEG C temperature extraction take, extract 3 times, each extraction time 3h obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder, spray tower
170~175 DEG C of air themperature, goes out 85~90 DEG C of tower air themperature, adds sodium carboxymethylcellulose, is made tablet.
Embodiment 7
Formula：Oldenlandia diffusa 32g, Sculellaria barbata 28g, phoenix-tail fern 29g, Verbena officinalis 11g, garlic 15g, cordate houttuynia 11g, Wu
Centipede extract 10g；
Preparation method：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to
Proportioning weighs each raw material, after being crushed with pulverizer respectively, crosses 150 mesh sieves, is put into mixer mixing；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 43~45 DEG C, extracting pressure
5MPa, extraction time 2h, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 4.5%, addition 1g/L saleratus, 4g/L potassium acetates
With 2) in extract, at 63~65 DEG C temperature extraction take, extract 3 times, each extraction time 2.5h obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder, spray tower
150~155 DEG C of air themperature, goes out 105~110 DEG C of tower air themperature, adds dextrin and sucrose, is made tablet.
Test example 1
Chronic toxicity test：
Experimental group：Group 1 takes small white mouse 10, and body weight 20g or so, male and female half and half are filled per daily drug dilution liquid of the present invention
Stomach, dosage：200mg/kg；Group 2 takes small white mouse 10, body weight 20g or so, male and female half and half, per daily drug dilution liquid of the present invention
Gavage, dosage：400mg/kg；Group 3 small white mouse 10, body weight 40g or so, male and female half and half, per daily drug dilution liquid of the present invention
Gavage, dosage：700mg/kg；Every group of continuous gavage 30 days, observes after being discontinued 14 days.
Control group：The 9g/L physiological saline of daily gavage equivalent.Continuous gavage 30 days, observes after being discontinued 14 days.
The mouse of 30 days processes of experiment, clinical observation test group and control group, the state of mind, diet, drinking-water, urination, defecation
And respond is showed no obvious abnormalities to external world, after being discontinued 14 days, mouse is catched and killed, collection blood separates serum, surveys
Determine the content of serum alt and AST, compared with control group, difference is not notable, all in normal range (NR).
After being discontinued 15 days, by cut open inspection, each group small white mouse heart, liver, spleen, lung, the form of kidney, color are normal, without substantially
Between naked eyes lesions visible, each group the heart of small white mouse, liver, spleen, lungs, Kidney coefficients difference not significantly, in illustrating this
Drug composition will not cause animal Solid organ injury or ANOMALOUS VARIATIONS.
By above testing and verification, the blood routine and organ coefficient of mouse, without significant difference, illustrate this hair with control group
Bright Chinese medicine composition will not produce chronic toxic action to small white mouse.
Acute toxicity test：
It is 40 small white mouses of 20g or so, male and female half and half, using drug dilution liquid gavage of the present invention, gavage to use body weight
Amount presses 0.5ml/10g, and the median lethal dose of Chinese medicine composition of the present invention can not be measured through prerun.Observation two weeks, the experiment of mouse
Activity is normal, and none mouse is dead, therefore drug oral toxicity very little of the present invention.
Test example 2
Take the logarithm growth period Hela cell, individual cells suspension is made with Trypsin Induced；With every hole 1 × 105/ ml is thin
Born of the same parents' concentration is inoculated in 96 well culture plates, per the μ L of pore volume 100, culture plate is moved into CO2In incubator, cultivate under optimum conditions
24h, after after cell attachment, test group adds medicine prepared by the embodiment of the present invention per hole, and blank group adds equivalent nutrient solution, 24h
Afterwards, the OD value (OD) of each group is measured at wavelength 570nm with automatic ELIASA.
To Hela cytosiies substantially, significantly, it suppresses medicine of the present invention for the medicine effect that wherein prepared by embodiment 3
Rate is 47.6%.
Claims (8)
1. a kind of Chinese medicine composition for treating cervical carcinoma, it is characterised in that raw material is in parts by weight：Oldenlandia diffusa 20~35
Part, 20~30 parts of Sculellaria barbata, 22~31 parts of phoenix-tail fern, Verbena officinalis parts 6~12 parts, 8~17 parts of garlic, 10~15 parts of cordate houttuynia,
5~10 parts of Scolopendra extract.
2. the Chinese medicine composition for the treatment of cervical carcinoma as claimed in claim 1, it is characterised in that the raw material is in parts by weight：
27 parts of oldenlandia diffusa, 25 parts of Sculellaria barbata, 26 parts of phoenix-tail fern, 7 parts of Verbena officinalis, 10 parts of garlic, 14 parts of cordate houttuynia, Scolopendra extract
8 parts.
3. the Chinese medicine composition of cervical carcinoma is treated as claimed in claim 1, it is characterised in that the Chinese medicine composition preparation method bag
Include following steps：
(1) dried after cleaning oldenlandia diffusa, Sculellaria barbata, phoenix-tail fern, Verbena officinalis, garlic, cordate houttuynia in raw material, according to proportioning
Each raw material is weighed, after being crushed with pulverizer respectively, 150~200 mesh sieves is crossed, mixer mixing is put into；
(2) raw material of mixing is used into supercritical carbon dioxide extraction method, extraction temperature is 42~48 DEG C, extracting pressure 5~
7MPa, 2~3h of extraction time, obtains extract；
(3) with the ethanol water that deionized water compound concentration is 4~7%, addition 5g/L solid inorganic salts and 2) in extract,
Temperature extraction takes at 60~70 DEG C, extracts 3 times, and each 2~3h of extraction time obtains extract solution；
(4) by 3) in extract solution mix with Scolopendra extract, and be condensed into thick paste, it is spray-dried into powder.
4. the Chinese medicine composition of cervical carcinoma is treated as claimed in claim 3, it is characterised in that the powder, by addition or not
Addition pharmaceutically acceptable carrier, is made different dosage forms.
5. the Chinese medicine composition of cervical carcinoma is treated as claimed in claim 3, it is characterised in that the solid inorganic salt is bicarbonate
One or more in potassium, sodium iodide, sodium chloride, potassium acetate.
6. the Chinese medicine composition of cervical carcinoma is treated as claimed in claim 3, it is characterised in that the spray drying, spray tower is empty
150~180 DEG C of temperature degree, goes out 80~110 DEG C of tower air themperature.
7. the Chinese medicine composition of cervical carcinoma is treated as claimed in claim 1, it is characterised in that the Scolopendra extract, its preparation
Method：Centipede cephalopodium, bamboo stick are removed, crushed after being dried uses absolute ethyl alcohol degreasing into 300 mesh, adds 6 times of Tris-
Triton-NaCl buffer solutions, 8h, high speed centrifugation 5min are oozed in cold at 4 DEG C, take green supernatant, are added equivalent to supernatant liquid measure
1.5 times of (NH4)2SO4, 10min is centrifuged again, incline supernatant, and Scolopendra extract is obtained after dialysis.
8. the Chinese medicine composition of cervical carcinoma is treated as claimed in claim 7, it is characterised in that the buffer solution is Tris-
In Triton-NaCl buffer solutions, PBS any one.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110151899A (en) * | 2019-06-28 | 2019-08-23 | 吴江平 | A kind of Chinese medicine draft formula and its fermentation preparation fighting HPV viruse |
| CN113456730A (en) * | 2021-06-09 | 2021-10-01 | 荣汝成 | Traditional Chinese medicine composition for treating cervical cancer precancerous lesion caused by HPV infection and preparation method thereof |
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| CN1228310A (en) * | 1998-03-09 | 1999-09-15 | 张富永 | Xiaoaisan powder for curing cancer |
| CN101125178A (en) * | 2007-08-27 | 2008-02-20 | 毕哲全 | Traditional Chinese medicine for treating tumor |
| CN102475822A (en) * | 2010-11-27 | 2012-05-30 | 冯文明 | Oral liquid capable of preventing and treating cervical cancer |
| CN103656041A (en) * | 2013-12-11 | 2014-03-26 | 张晨 | Traditional Chinese medicine for treating cervical cancer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1228310A (en) * | 1998-03-09 | 1999-09-15 | 张富永 | Xiaoaisan powder for curing cancer |
| CN101125178A (en) * | 2007-08-27 | 2008-02-20 | 毕哲全 | Traditional Chinese medicine for treating tumor |
| CN102475822A (en) * | 2010-11-27 | 2012-05-30 | 冯文明 | Oral liquid capable of preventing and treating cervical cancer |
| CN103656041A (en) * | 2013-12-11 | 2014-03-26 | 张晨 | Traditional Chinese medicine for treating cervical cancer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110151899A (en) * | 2019-06-28 | 2019-08-23 | 吴江平 | A kind of Chinese medicine draft formula and its fermentation preparation fighting HPV viruse |
| CN113456730A (en) * | 2021-06-09 | 2021-10-01 | 荣汝成 | Traditional Chinese medicine composition for treating cervical cancer precancerous lesion caused by HPV infection and preparation method thereof |
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