CN106725992B - A kind of passive smoking device for being used to prepare Chronic Obstructive Pulmonary Disease animal model - Google Patents

Abstract

The present invention specifically relates to a passive smoking device for preparing an animal model of chronic obstructive pulmonary disease, which mainly solves the problem of local high temperature and uncontrollable smoke concentration caused by burning cigarettes in existing preparation devices for animal models of chronic obstructive pulmonary disease , Uneven technical problems. The present invention mainly consists of an animal smoke receiving box, a flue inlet and a smoke generating device composed of a movable top cover, a side plate and a movable grid bottom plate. The smoke port is connected with the bottom of the movable grid bottom plate of the animal smoke box, and the smoke inlet of the flue inlet is connected with the smoke outlet of the smoke generating device. The present invention treats animals (rats, mice, mice, etc.) Guinea pigs) to conduct continuous passive smoking experiments to make animal models of chronic obstructive pulmonary disease caused by passive smoking. It is easy to operate, high in work efficiency, simple in structure, and low in cost. use.

Description

A passive smoking device for preparing animal models of chronic obstructive pulmonary disease

technical field

The invention belongs to the technical field of experimental devices, in particular to a passive smoking device for preparing animal models of chronic obstructive pulmonary disease.

Background technique

Chronic obstructive pulmonary disease (COPD) is a common and frequently occurring respiratory disease in my country, causing chronic irreversible airway obstruction, increased respiratory resistance and pulmonary insufficiency caused by damage to the lung parenchyma, such as small airways. The characteristics of lung diseases are collectively referred to, and the fatality rate is high, which seriously affects the labor ability and quality of life of patients. COPD is currently the fourth leading cause of death in the world, and will rank fifth in the world's economic burden of disease by 2020, and the third leading cause of death in the world. Smoke (including smoke from burning cigarettes, smoke from burning biological waste (such as shavings, sawdust, straw, branches), smoke from burning coal and organic materials (such as PVC, PE pipes)) is an important aspect that causes COPD. Its ingredients contain a variety of harmful ingredients, which can damage the epithelial cells of the bronchial mucosa, weaken the phagocytic ability of alveolar macrophages, cause oxidative stress in the lungs, lead to intracellular lipid peroxidation and inflammation, etc., but its exact The pathogenesis has not yet been elucidated, so the establishment of a long-term, stable disease animal model that simulates human COPD can provide an important technical means for in-depth research on the pathogenesis of COPD and the search for prevention and treatment drugs. However, the COPD animal model established by the traditional smoking modeling method applied at home and abroad has many shortcomings, such as: there is cigarette smoke stimulation and other stimulating factors (such as endotoxin); The degree of smoke invasion of a single animal; the identity between different batches of animals cannot be guaranteed; the damage to lung tissue caused by passive smoking (to ensure the oxygen concentration in the air is within the normal range) under natural conditions cannot be simulated; some modeling equipment does not take into account the burning of cigarettes The effect of temperature on the device and animals. Therefore, the existing model is actually an acute or subacute COPD model, which cannot truly reflect the pathological damage of COPD caused by passive smoking in actual situations, nor can it be used to evaluate the efficacy of some potential drugs, and is not suitable for rough drug screening and small and medium-sized patients. Used by laboratories and small and medium enterprises.

Contents of the invention

The purpose of the present invention is to solve the technical problems of the local high temperature effect and the uncontrollable and uneven smoke concentration generated when the cigarette is burned in the existing preparation device of the chronic obstructive pulmonary disease animal model, and to provide a method for preparing the chronic obstructive pulmonary disease animal model. Passive smoking device for animal models.

In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:

A passive smoking device for preparing an animal model of chronic obstructive pulmonary disease, comprising an animal smoke receiving box, a flue inlet and a smoke generating device composed of a movable top cover, a side panel and a movable grid bottom panel, in the activity The top of the top cover is provided with a smoke outlet, the smoke outlet of the flue inlet is connected with the bottom of the movable mesh bottom plate of the animal smoke receiving box, and the smoke inlet of the flue inlet is connected with the smoke outlet of the smoke generating device.

The passive smoking device for preparing the animal model of chronic obstructive pulmonary disease also includes an adjustable speed fan, and the adjustable speed fan is arranged on the smoke outlet on the top of the movable top cover to adjust the speed (concentration) of entering/exiting smoke .

The passive smoking device for preparing the animal model of chronic obstructive pulmonary disease also includes a smoke sensor, which is arranged on the inner wall of the side panel of the animal smoking box for measuring the smoke concentration in the animal smoking box. When the smoke concentration is too high (such as the oxygen content is lower than 21%), the smoke sensor will give an alarm, and at the same time, the linkage device will increase the speed of the adjustable fan to accelerate the discharge of smoke.

The passive smoking device for preparing the animal model of chronic obstructive pulmonary disease also includes handles arranged on the movable top cover and the outer wall of the side plate of the animal smoking box, so as to move the passive smoking device. At the same time, the existence of the handle can facilitate the putting in and taking out of the test animal and the cleaning and replacement of the movable grid bottom plate.

The passive smoking device used to prepare the animal model of chronic obstructive pulmonary disease also includes a filter membrane, which is laid on the upper surface or the lower surface of the movable grid bottom plate, and is used to prevent the smoke from being discharged under the action of the adjustable speed fan. Passing through the animal smoke enclosure too quickly. The filter membrane is made of 100 or 200-mesh nylon membrane, which is convenient to control the speed of the smoke entering the animal smoke receiving box, and can avoid the formation of a circuit directly under the fan when the smoke enters the animal smoke receiving box due to too fast fan speed, causing the smoke to flow into the animal smoke receiving box. The smoke box is unevenly distributed.

The movable top cover or the flue inlet is conical, or the movable top cover and the flue inlet are conical, which can effectively make the smoke enter the animal smoke receiving box. The flue inlet is conical, which helps the smoke evenly enter the animal smoke box.

The smoke generating device is composed of an inner box, an outer box and at least one smoke chamber, one side of the inner box is provided with a smoke inlet, one side of the outer box is provided with an air inlet, and the inner box is It is arranged in the inner cavity of the outer box, and communicates with the smoke outlet on the top of the inner box and the outer box, and makes the smoke inlet of the inner box and the air inlet of the outer box on the same side, the at least A smoke chamber is arranged in the cavity between the inner box body and the outer box body. As an independent structure, the smoke chamber can effectively isolate the high temperature generated during smoke burning; the amount of smoke produced can be adjusted by adjusting the number of smoke chambers.

A smoke baffle is arranged at the front of the smoke port of the inner box, so that the smoke generated by the smoke chamber can enter the smoke inlet of the inner box from one direction. The existence of the smoke baffle ensures that the smoke is fully mixed with the air before entering the smoke inlet, and ensures that the smoke entering the animal smoke receiving box is uniform.

A sliding door is arranged on the air inlet of the outer box so as to adjust the air intake. The size of the opening of the sliding door is adjusted according to the value of the smoke sensor on the inner wall of the animal smoke box. Reducing the opening can reduce the air intake, inhibit the burning of materials in the smoke chamber, reduce the generation of smoke, and ensure that the animal smoke box Oxygen content, on the contrary will increase the generation of smog.

A support column is provided between the animal smoke receiving box and the smoke generating device for stabilizing the whole device.

The present invention adopts the above technical scheme, greatly optimizes the preparation process of the chronic obstructive pulmonary disease animal model, and simulates the natural situation of passive smoking to the greatest extent. The independent setting of the smoke chamber not only provides a source of various types of smoke, but also effectively isolates the potential impact of the local high temperature generated during the smoke on the smoke device and the tested animals; the smoke inlet of the inner box The special smoke baffle can make the smoke generated by the smoke chamber flow in one direction only, and can ensure that the smoke is fully mixed with the air before entering the smoke inlet, so that the smoke entering the animal smoke receiving box is uniform. The inverted cone-shaped structure of the flue inlet and the existence of the filter membrane ensure that the smoke evenly enters the animal smoke receiving box and is evenly distributed; The size of the sliding door opening on the tuyere and multiple measures are taken simultaneously to ensure the oxygen content in the air in the animal smoking box, to simulate passive smoking in natural conditions to the greatest extent, and to effectively protect the basic welfare of the tested animals; further, activities The top cover and the handle on the outer wall of the animal smoke receiving box are convenient for moving the box, and are convenient for cleaning and replacing the movable grid bottom plate and filter membrane in the box.

Description of drawings

Fig. 1 is the structural representation of the first embodiment of the present invention;

Fig. 2 is the structural representation of the second embodiment of the present invention;

Fig. 3 is a schematic structural view of the smoke generating device of the present invention;

Detailed ways

Example 1

As shown in Figure 1, a passive smoking device used to prepare an animal model of chronic obstructive pulmonary disease in this embodiment includes an animal receiving device consisting of a conical movable top cover 1, a side plate 2 and a movable grid bottom plate 3. The smoke box body, the inverted conical flue inlet 4 and the smoke generating device 5 are provided with a smoke outlet 6 on the top of the conical movable top cover 1, and the wide mouth smoke outlet of the inverted conical flue inlet 4 is connected with the animal The bottom of the movable grid bottom plate 3 of the smoke receiving box is connected, and the narrow smoke inlet of the inverted conical flue inlet 4 is connected with the smoke outlet of the smoke generating device 5 .

The passive smoking device for preparing animal models of chronic obstructive pulmonary disease also includes an adjustable speed fan 7 , which is arranged on the smoke outlet 6 on the top of the movable top cover 1 .

The passive smoking device for preparing animal models of chronic obstructive pulmonary disease also includes a smoke sensor 8, which is arranged on the inner wall of the side plate 2 of the animal smoking box.

The passive smoking device for preparing animal models of chronic obstructive pulmonary disease also includes a handle 9 provided on the outer wall of the conical movable top cover 1 and the side plate 2 of the animal smoking box.

The passive smoking device for preparing animal models of chronic obstructive pulmonary disease also includes a filter membrane laid on the upper surface of the movable grid bottom plate 3 .

As shown in Figure 3, the smoke generating device 5 is composed of an inner box body 501, an outer box body 502 and at least one smoke chamber 503, a smoke inlet 504 is provided on one side of the inner box body 501, and a smoke inlet 504 is provided on the outer box body 502. One side of the air inlet 505 is provided, and the inner box 501 is arranged in the inner cavity of the outer box 502, and the smoke outlet 506 arranged on the top of the inner box 501 and the outer box 502 is communicated, and the inner box The smoke inlet 504 of the body 501 and the air inlet 505 of the outer box 502 are located on the same side, and the at least one smoke chamber 503 is arranged in the cavity between the inner box 501 and the outer box 502 .

A smoke baffle 507 is provided at the front of the smoke inlet 504 of the inner box 501 .

A sliding door is arranged on the air inlet 505 of the outer box body 502 .

Example 2

As shown in Figure 2, a passive smoking device for preparing an animal model of chronic obstructive pulmonary disease in this embodiment includes a support column 10 arranged between the animal smoking box and the smoke generating device 5, and other Same as Example 1.

The filter membrane 10 in the above embodiments can also be laid on the lower surface of the movable grid bottom plate 3 .

The movable top cover 1 or the flue inlet 4 in the above-mentioned embodiment can choose one to be tapered.

During use, cigarettes, biological waste or organic plastics are placed in the smoke chamber 503, and a certain opening is reserved by adjusting the sliding door on the air inlet 505 of the outer box body 502; Rats or 12 mice or 4 guinea pigs) are placed in the animal smoke box, cover the conical movable top cover 1, open the adjustable speed fan 7, the experiment officially begins, and record the start time of the experiment; during the experiment, by The smoke sensor 8 monitors the smoke concentration in the smoke box to ensure that the tested animals are in a natural environment. If the smoke concentration is too high, the oxygen concentration will decrease. When it is lower than 21%, adjust the speed of the fan 7 or adjust the outer box. The sliding door on the air inlet 505 of 502 adjusts the smoke intake to ensure that the oxygen concentration is always in the normal range; the test animals are passively smoked once a day in the morning and afternoon, and each box is lit with 6 commercially available non-filter cigarettes each time , or 1 jin of biological waste or 1 jin of organic plastics, each experiment takes half an hour. In actual operation, the species and quantity of the tested animals can also be adjusted according to the needs of the experiment, and the time and frequency of the experiment can be arranged.

In this example, the animal model device for chronic obstructive pulmonary disease caused by passive smoking uses domestic plexiglass as the main raw material for the animal receiving box and the smoke generating device, supplemented by asbestos gaskets. The preparation process is simple, the price is low, and it is conducive to large-scale production. The smoke chamber is made of stainless steel, which can effectively prevent open flames and effectively isolate the impact of the high temperature generated during combustion on the tested animals and damage to the organic box. It is also simple, convenient, and easy to clean. Multiple devices can be used at the same time, greatly improving the quality of life. work efficiency.

The protection scope of the present invention is not limited by the above embodiments.

Claims (3)

1. a kind of passive smoking device for being used to prepare Chronic Obstructive Pulmonary Disease animal model, it is characterised in that：Including by living The animal of dynamic head cover (1), side plate (2) and moving mesh bottom plate (3) composition is by smoke box body, flue (4) and smoke generating device (5), outlet flue (6) is equipped at the top of the removable top cover (1), the outlet flue of flue (4) is with animal by the work of smoke box body The bottom connection of dynamic mesh bottom plate (3), the smoke inlet of flue (4) are connected with the outlet flue of smoke generating device (5)；The use Speed adjustable fan (7), speed adjustable fan (7) are further included in the passive smoking device for preparing Chronic Obstructive Pulmonary Disease animal model It is located on the outlet flue (6) at the top of removable top cover (1)；The passive suction for being used to prepare Chronic Obstructive Pulmonary Disease animal model Cigarette device further includes smoke inductor (8), and the smoke inductor (8) is located at inner wall of the animal by cigarette box side (2)；It is described The passive smoking device for being used to prepare Chronic Obstructive Pulmonary Disease animal model further includes and is located at animal by smoke box body removable top cover (1) and the handle (9) of side plate (2) outer wall；The passive smoking device for being used to prepare Chronic Obstructive Pulmonary Disease animal model Filter membrane is further included, the filter membrane is laid on the upper surface or lower surface of moving mesh bottom plate (3)；The removable top cover (1) or into Flue (4) is taper or removable top cover (1) and flue (4) is taper；The smoke generating device (5) is by internal box (501), outer case (502) and at least one burning smoke storehouse (503) composition, smoke inlet is equipped in the one side of internal box (501) (504), air inlet (505) is equipped in the one side of outer case (502), the internal box (501) is located at the inner cavity of outer case (502) In, and make to be located at internal box (501) and be connected with the outlet flue (506) at the top of outer case (502), and make internal box (501) into Mouth (504) and the air inlet (505) of outer case (502) are located at the same side, and at least one burning smoke storehouse (503) is arranged on interior case In cavity between body (501) and outer case (502)；Smog is equipped in the forepart of the smoke inlet (504) of the internal box (501) Baffle (507).

2. a kind of passive smoking device for being used to prepare Chronic Obstructive Pulmonary Disease animal model according to claim 1, It is characterized in that：Sliding door is equipped on the air inlet (505) of the outer case (502).

3. a kind of passive smoking dress for being used to prepare Chronic Obstructive Pulmonary Disease animal model according to claim 1 or 2 It puts, it is characterised in that：Support column (10) is equipped between by smoke box body and smoke generating device (5) in the animal.