CN106718935A - The method taken root in tung oil tree tissue-cultured seedling bottle - Google Patents
The method taken root in tung oil tree tissue-cultured seedling bottle Download PDFInfo
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- CN106718935A CN106718935A CN201710077542.2A CN201710077542A CN106718935A CN 106718935 A CN106718935 A CN 106718935A CN 201710077542 A CN201710077542 A CN 201710077542A CN 106718935 A CN106718935 A CN 106718935A
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- 240000001866 Vernicia fordii Species 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000005286 illumination Methods 0.000 claims abstract description 18
- 238000013138 pruning Methods 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 238000005728 strengthening Methods 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims description 12
- 229920001817 Agar Polymers 0.000 claims description 10
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 10
- 239000008272 agar Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 10
- 102100040431 Activator of basal transcription 1 Human genes 0.000 claims description 5
- 241000206575 Chondrus crispus Species 0.000 claims description 5
- 239000004471 Glycine Substances 0.000 claims description 5
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 5
- 101000964349 Homo sapiens Activator of basal transcription 1 Proteins 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 5
- 239000007836 KH2PO4 Substances 0.000 claims description 5
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 5
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 5
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 5
- 229960000367 inositol Drugs 0.000 claims description 5
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 229960003512 nicotinic acid Drugs 0.000 claims description 5
- 235000001968 nicotinic acid Nutrition 0.000 claims description 5
- 239000011664 nicotinic acid Substances 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 5
- 239000011684 sodium molybdate Substances 0.000 claims description 5
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 229940088594 vitamin Drugs 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 4
- 230000035755 proliferation Effects 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000010276 construction Methods 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 4
- 239000002994 raw material Substances 0.000 description 3
- 238000010008 shearing Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 244000055346 Paulownia Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241001164374 Calyx Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 244000147058 Derris elliptica Species 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- 244000057114 Sapium sebiferum Species 0.000 description 1
- 235000005128 Sapium sebiferum Nutrition 0.000 description 1
- 241000238370 Sepia Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 244000153888 Tung Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021038 drupes Nutrition 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Method the invention discloses being taken root in a kind of tung oil tree tissue-cultured seedling bottle, choose the tung oil tree subculture bud through strengthening 35~40d of bud culture in conventional tissue culture, pre- culture of rootage is first carried out after sterilizing and pruning, by carrying out culture of rootage again after the pre- culture of rootage of 30~35d, it is placed in 20 ± 1 DEG C of temperature, humidity 55~70%, 2000~2500lux of intensity of illumination, cultivated in the environment of 15~16h/d of illumination, obtain band root tissue-cultured seedling.Taken root the cycle present invention reduces tung oil tree subculture bud, rooting rate is high, and root system is more, quality is good, and tissue-cultured seedling transplanting survival rate of taking root is high, is capable of achieving tung oil tree tissue culture industrialization nursery, for the construction of artificial clone woods provides high quality seedling, with preferable economic benefit, social benefit and ecological benefits.
Description
Technical field
The invention belongs to tung oil tree vegetative propagation technique, a kind of method for being related to be taken root in tung oil tree tissue-cultured seedling bottle.
Background technology
Tung oil tree（Vernicia fordii (Hemsl.) Airy Shaw）, Euphorbiaceae tung tree, category deciduous tree, bark
Grey, branch is sturdy, leaf oval, and flower monoecism, calyx outside is close by the micro- pubescence of sepia；Petal white, there is pale red
Vein, obovate, ovary is close by pubescence, and drupe is closely spherical, plants skin wooden.The flowering fruit bearing stage 3-9 months.It is distributed in Shaanxi, the river of China
South, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Hubei, Guangdong, Hainan, Guangxi, Sichuan, Guizhou, Yunnan etc. save
Area.Tung oil tree and oil tea, walnut, Chinese tallow tree simultaneously claim Chinese four big traditional oil trees.Tung oil tree is born in more than 1000 meters of area, happiness temperature
It is warm, avoid severe cold.18 DEG C of winter temperature drop is conducive to tung oil tree to grow, but being in less than -10 DEG C can cause jelly for a long time
Evil.It is suitable to be born in gentle slope and on the sunny side valley floor, basin and riverbed two sides tableland.Rich in humus, soil layer is deep, draining is good, neutral
To the most suitable tung oil tree growth of subacidity sandy loam.
Tung oil tree planting type is made between having paulownia agriculture, builds work etc. between pure forest, fragmentary plantation and woods paulownia.Tissue culture technique is one
Fast asexual propagation technology is planted, selection tung oil tree superior families or clone are material, and nursery stock is bred by method for tissue culture,
Both the quantitative requirement in production can be met, maternal character can be kept again.But during tissue culture, usually there is rooting rate
Low, disunity of taking root, the problem that root quantity is few and root system is not sturdy.
The content of the invention
It is an object of the invention to provide a kind of rooting rate that can improve tung oil tree tissue-cultured seedling, root quantity and root system are sprouted
The method taken root in the tung oil tree tissue-cultured seedling bottle of regularity.
In order to realize foregoing invention purpose, technical scheme is as follows：
A kind of method taken root in tung oil tree tissue-cultured seedling bottle, chooses proliferation and subculture tung oil tree seedling, first carries out pre- culture of rootage, Ran Houzai
Culture of rootage is carried out, is placed in control environment and is cultivated, obtain band root tissue-cultured seedling, main operational steps are as follows：
（1）Materials：The tung oil tree subculture bud through strengthening 35~40d of bud culture in conventional tissue culture is chosen, after sterilization bottle appearance, ultra-clean
In sterilized space on workbench, robust growth, the simple bud of 1~2cm of height in subculture bud clump are chosen, cut at the lower 2~3mm of section
Cut, remove radical leaves and petiole, it is standby；
（2）Pre- culture of rootage：By step（1）Simple bud after pruning is inoculated in pre- root media, in specific photo-thermal reaction
In carry out pre- culture of rootage；
（3）Culture of rootage：Treat step（2）In simple bud by after the pre- culture of rootage of 30~35d, simple bud being transferred into culture of rootage
Base, carries out culture of rootage in specific photo-thermal reaction.
Above-described its material content of pre- root media is：1/2 1.0~2.0mg/L+ of improvement ER culture medium+IBA
1.0~2.0mg/L of vitamin C 6g/L++VC+carragheen 25g/L+ agar 5.0g/L.
Above-described root media its material content is：1/2 1.0~2.0mg/L+ of improvement ER culture medium+IAA
ABT1#0.5~2.0 mg/L+ sucrose 25g/L+ agar 5.0g/L.
The formula of above-described improvement ER culture mediums is：NH4NO3 1150mg/L + KNO3 980mg/L +
CaCl2.2H2O 440mg/L + MgSO4..7H2O 370mg/L + KH2PO4 440mg/L + H3BO3 0.63mg/L +
MnSO4..4H2O 20.9mg/L + ZnSO4.4H2O 7.56H2Omg/L + Na2MoO4.H2O 0.025mg/L +
CuSO4..5H2O 0.0025mg/L + CoCl2.6H2O 0.0025mg/L + FeSO4 27.8mg/L + NaEDTA
37.3mg/L+inositol 50mg/L+nicotinic acid 0.5mg/L+puridoxine hydrochloride 0.5mg/L+glycine 2mg/L.
Above step（2）And step（3）Described photo-thermal reaction is：20 ± 1 DEG C of temperature, humidity 55~70%, intensity of illumination
2000~2500lux, 15~16h/d of illumination.
The present invention has the advantage that and has the beneficial effect that：
1st, the present invention improves ER culture mediums as minimal medium using 1/2, at the same emphasis have adjusted it is related to what tung oil tree took root
Trace element and organic principle so that culture medium is more scientific, more targetedly, is more easy to promote tung oil tree subculture bud to take root, effect
Significantly.
2nd, the present invention uses low concentration IBA and ascorbic acid before rooting induction（VC）Pre- training of taking root is carried out to subculture simple bud
Support, culture of rootage is then carried out again, can unify tissue-cultured seedling root of hair, root of hair speed is fast, and root system is sturdy and quantity is more.
3rd, the present invention in proliferation and subculture simple bud by being transferred to culture of rootage after 30~35d times pre- culture of rootage, you can reach
To pre- culture of rootage effect, turn avoid seedling and grow root system in the pre- culture of rootage stage and make later stage culture of rootage root of hair not whole
Together.
4th, it is of the invention that the pre- growth taken root and culture of rootage, adapt to tung oil tree tissue-cultured seedling is carried out under specific light and temperature condition
Characteristic, promotes the growth of nursery stock.
5th, taken root the cycle present invention reduces tung oil tree subculture bud, rooting rate is high, and root system is more, quality is good, tissue-cultured seedling of taking root is moved
Plant survival rate high, tung oil tree tissue culture industrialization nursery is capable of achieving, for the construction of artificial clone woods provides high quality seedling, with preferable
Economic benefit, social benefit and ecological benefits.
Specific embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1：
The tung oil tree subculture bud through strengthening 35~40d of bud culture in conventional tissue culture is chosen, after sterilization bottle appearance, on superclean bench
Sterilized space in, robust growth, the simple bud of 1~2cm of height in subculture bud clump are chosen, in the lower 2~3mm places shearing of section, removing
Radical leaves and petiole.Simple bud after pruning is inoculated in pre- root media, described its raw material of pre- root media contains
Measure and be：1/2 improvement ER culture medium+IBA 1.0mg/L+ vitamin Cs 6g/L++VC 2.0mg/L+carragheen 25g/L+ agar
5.0g/L, in 20 ± 1 DEG C of temperature, humidity 55~60%, intensity of illumination 2000lux carries out pre- taking root in the environment of illumination 16h/d
Culture.After simple bud by after the pre- culture of rootage of 30~35d, simple bud being transferred into root media, described root media its
Material content is：1/2 improvement ER culture medium+IAA 1.0mg/L+ABT1#0.5 mg/L+ sucrose 25g/L+ agar 5.0g/L,
20 ± 1 DEG C of temperature, humidity 55~60%, intensity of illumination 2000lux carries out culture of rootage in illumination 16h/d environment.Culture 15-
20d, more than 90% simple bud grows root system.
The formula of above-described improvement ER culture mediums is：NH4NO3 1150mg/L + KNO3 980mg/L +
CaCl2.2H2O 440mg/L + MgSO4..7H2O 370mg/L + KH2PO4 440mg/L + H3BO3 0.63mg/L +
MnSO4..4H2O 20.9mg/L + ZnSO4.4H2O 7.56H2Omg/L + Na2MoO4.H2O 0.025mg/L +
CuSO4..5H2O 0.0025mg/L + CoCl2.6H2O 0.0025mg/L + FeSO4 27.8mg/L + NaEDTA
37.3mg/L+inositol 50mg/L+nicotinic acid 0.5mg/L+puridoxine hydrochloride 0.5mg/L+glycine 2mg/L.
Embodiment 2：
The tung oil tree subculture bud through strengthening 35~40d of bud culture in conventional tissue culture is chosen, after sterilization bottle appearance, on superclean bench
Sterilized space in, robust growth, the simple bud of 1~2cm of height in subculture bud clump are chosen, in the lower 2~3mm places shearing of section, removing
Radical leaves and petiole.Simple bud after pruning is inoculated in pre- root media, described its raw material of pre- root media contains
Measure and be：1/2 improvement ER culture medium+IBA 1.5mg/L+ vitamin Cs 6g/L++VC 1.0mg/L+carragheen 25g/L+ agar
5.0g/L, in 20 ± 1 DEG C of temperature, humidity 60~65%, intensity of illumination 2000lux carries out pre- taking root in the environment of illumination 15h/d
Culture.After simple bud by after the pre- culture of rootage of 30~35d, simple bud being transferred into root media, described root media its
Material content is：1/2 improvement ER culture medium+IAA 1.5mg/L+ABT1#1.0 mg/L+ sucrose 25g/L+ agar 5.0g/L,
20 ± 1 DEG C of temperature, humidity 60~65%, intensity of illumination 2000lux carries out culture of rootage in the environment of illumination 15h/d.Culture 15-
20d, more than 90% simple bud grows root system.
The formula of above-described improvement ER culture mediums is：NH4NO3 1150mg/L + KNO3 980mg/L +
CaCl2.2H2O 440mg/L + MgSO4..7H2O 370mg/L + KH2PO4 440mg/L + H3BO3 0.63mg/L +
MnSO4..4H2O 20.9mg/L + ZnSO4.4H2O 7.56H2Omg/L + Na2MoO4.H2O 0.025mg/L +
CuSO4..5H2O 0.0025mg/L + CoCl2.6H2O 0.0025mg/L + FeSO4 27.8mg/L + NaEDTA
37.3mg/L+inositol 50mg/L+nicotinic acid 0.5mg/L+puridoxine hydrochloride 0.5mg/L+glycine 2mg/L.
Embodiment 3：
The tung oil tree subculture bud through strengthening 35~40d of bud culture in conventional tissue culture is chosen, after sterilization bottle appearance, on superclean bench
Sterilized space in, robust growth, the simple bud of 1~2cm of height in subculture bud clump are chosen, in the lower 2~3mm places shearing of section, removing
Radical leaves and petiole.Simple bud after pruning is inoculated in pre- root media, described its raw material of pre- root media contains
Measure and be：1/2 improvement ER culture medium+IBA 2.0mg/L+ vitamin Cs 6g/L++VC 1.5mg/L+carragheen 25g/L+ agar
5.0g/L, in 20 ± 1 DEG C of temperature, humidity 65~70%, intensity of illumination 2500lux carries out pre- taking root in the environment of illumination 15h/d
Culture.After simple bud by after the pre- culture of rootage of 30~35d, simple bud being transferred into root media, described root media its
Material content is：1/2 improvement ER culture medium+IAA 2.0mg/L+ABT1#2.0 mg/L+ sucrose 25g/L+ agar 5.0g/L,
20 ± 1 DEG C of temperature, humidity 65~70%, intensity of illumination 2500lux carries out culture of rootage in the environment of illumination 15h/d.Culture 15-
20d, more than 90% simple bud grows root system.
The formula of above-described improvement ER culture mediums is：NH4NO3 1150mg/L + KNO3 980mg/L +
CaCl2.2H2O 440mg/L + MgSO4..7H2O 370mg/L + KH2PO4 440mg/L + H3BO3 0.63mg/L +
MnSO4..4H2O 20.9mg/L + ZnSO4.4H2O 7.56H2Omg/L + Na2MoO4.H2O 0.025mg/L +
CuSO4..5H2O 0.0025mg/L + CoCl2.6H2O 0.0025mg/L + FeSO4 27.8mg/L + NaEDTA
37.3mg/L+inositol 50mg/L+nicotinic acid 0.5mg/L+puridoxine hydrochloride 0.5mg/L+glycine 2mg/L.
Claims (5)
1. a kind of method taken root in tung oil tree tissue-cultured seedling bottle, it is characterised in that：Proliferation and subculture tung oil tree seedling is chosen, pre- life is first carried out
Root culture, then carries out culture of rootage again, is placed in control environment and cultivates, and obtains band root tissue-cultured seedling, and main operational steps are as follows：
（1）Materials：The tung oil tree subculture bud through strengthening 35~40d of bud culture in conventional tissue culture is chosen, after sterilization bottle appearance, ultra-clean
In sterilized space on workbench, robust growth, the simple bud of 1~2cm of height in subculture bud clump are chosen, cut at the lower 2~3mm of section
Cut, remove radical leaves and petiole, it is standby；
（2）Pre- culture of rootage：By step（1）Simple bud after pruning is inoculated in pre- root media, in specific photo-thermal reaction
In carry out pre- culture of rootage；
（3）Culture of rootage：Treat step（2）In simple bud by after the pre- culture of rootage of 30~35d, simple bud being transferred into culture of rootage
Base, carries out culture of rootage in specific photo-thermal reaction.
2. the method taken root in a kind of tung oil tree tissue-cultured seedling bottle according to claim 1, it is characterised in that：It is described pre- to take root
Culture medium its material content is：1/2 improvement ER culture medium+IBA 1.0~2.0mg/L+ vitamin Cs 6g/L++VC 1.0~
2.0mg/L+carragheen 25g/L+ agar 5.0g/L.
3. the method taken root in a kind of tung oil tree tissue-cultured seedling bottle according to claim 1, it is characterised in that：Described training of taking root
Supporting base its material content is：1/2 improvement ER culture medium+IAA 1.0~2.0mg/L+ABT1#0.5~2.0 mg/L+ sucrose 25g/
L+ agar 5.0g/L.
4. according to the method taken root in a kind of any described tung oil tree tissue-cultured seedling bottle of Claims 2 or 3, it is characterised in that：It is described
The formula of improvement ER culture mediums be：NH4NO3 1150mg/L + KNO3 980mg/L + CaCl2.2H2O 440mg/L +
MgSO4..7H2O 370mg/L + KH2PO4 440mg/L + H3BO3 0.63mg/L + MnSO4..4H2O 20.9mg/L +
ZnSO4.4H2O 7.56H2Omg/L + Na2MoO4.H2O 0.025mg/L + CuSO4..5H2O 0.0025mg/L +
CoCl2.6H2O 0.0025mg/L + FeSO427.8mg/L+NaEDTA 37.3mg/L+inositol 50mg/L+nicotinic acid
0.5mg/L+puridoxine hydrochloride 0.5mg/L+glycine 2mg/L.
5. the method taken root in a kind of tung oil tree tissue-cultured seedling bottle according to claim 1, it is characterised in that：Step（2）And step
Suddenly（3）Described photo-thermal reaction is：20 ± 1 DEG C of temperature, humidity 55~70%, 2000~2500lux of intensity of illumination, illumination 15~
16h/d。
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106359103A (en) * | 2016-10-17 | 2017-02-01 | 柳州玲通科技有限责任公司 | Lithocarpus elmerrillii tissue culture seedling rooting induction method |
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