CN106718747A - A kind of cuttage and seedling culture method of the lignifying middle part branch of right cylinder fruit tree - Google Patents
A kind of cuttage and seedling culture method of the lignifying middle part branch of right cylinder fruit tree Download PDFInfo
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- CN106718747A CN106718747A CN201611155671.0A CN201611155671A CN106718747A CN 106718747 A CN106718747 A CN 106718747A CN 201611155671 A CN201611155671 A CN 201611155671A CN 106718747 A CN106718747 A CN 106718747A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Abstract
The invention discloses a kind of cuttage and seedling culture method of the lignifying middle part branch of right cylinder fruit tree, belong to technical field of plant asexual propagation.The cuttage and seedling culture method step is as follows：Select health and trunk leads to straight right cylinder fruit tree for elite stand, using lignifying branch from the branch place 8~15cm of interception apart from 6~60cm of its top long as cuttings, be soaked in water cuttings, sterilization；Cuttings bottom is put into plant growth regulator treatment liquid and is soaked, then cuttage is cultivated in mixed-matrix.Nursery is carried out using the inventive method, right cylinder fruit tree branch is breached because of tannin content technical bottleneck high and difficult to take root, start to take root within 30 35 days after the branch cutting of its lignifying middle part, can transplant within 45 months, effectively shorten the required time of taking root, the rooting rate and transplanting success of cuttage are significantly improved, cuttage rooting quality is good；Merit can be kept, it is reliable and stable, beneficial to popularization, with broader development prospect.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, the lignifying middle part branch of more particularly to a kind of right cylinder fruit tree
Cuttage and seedling culture method.
Background technology
Right cylinder fruit tree（Conocarpus erectus(L.)）, it is combretaceae（Combretaceae）Aggregate fruit belongs to
（Conocarpus）Seeds, aiphyllium.
Right cylinder fruit tree natural distributed is in America, and tree-like logical straight, tree crown is graceful, highly moderate（6~10 m）, can be on beach
Growth is applied, can be settled down on land again and beautified the environment, medical value also very high（Removing toxic substances preventing malaria, cures mainly malaria）, in U.S.'s Buddhist
There is more plantation Flo-Rida-Low, Mexico California, relatively resist cold.Especially in Florida Coastal beach trailing edge, in flakes
Neat single right cylinder fruit tree woods, is mainly used as the windproof unrestrained forest belt that disappears, and resists the attack of typhoon storm tide.While right cylinder fruit tree
It is also one of chief species for being seen everywhere as courtyard greening in avenue.
Right cylinder fruit tree introduced China in 2010 from the U.S..Current its modes of reproduction mainly has seminal propagation and cuttage numerous
Grow, seminal propagation germination percentage about 30%.It is at present, domestic actually rare to the research of the vegetative propagation technology of right cylinder fruit tree,
Cuttage generally is carried out using shoot, but there are problems that cutting plantation and survival rate are low.
The present invention passes through vegetative propagation technology, especially from the lignifying middle part branch of right cylinder fruit tree, can
Solve because seed-setting with yield poorly, the problems such as genetic variation and genetic differentiation is big, improve its germination percentage（15% is at least improved than seminal propagation）,
For the preservation and exploitation of right cylinder fruit tree resource open new way.
The content of the invention
It is an object of the invention to provide a kind of cuttage and seedling culture method of the lignifying middle part branch of right cylinder fruit tree.
The technical solution used in the present invention is：
A kind of cuttage and seedling culture method of right cylinder fruit tree, comprises the following steps：
1) preparation of cuttings：Select health and trunk is logical straight, right cylinder of the diameter of a cross-section of a tree trunk 1.3 meters above the ground not less than 8.0 cm, the height of tree not less than 6.5m is really
Wood is elite stand, using lignifying branch that lower end footpath is 1.0 cm~2.0 cm as cuttings material；
2) preparation and cuttage of cuttings：By step 1) the lignifying branch that obtains, cuts from apart from the place of 6~60cm of its top
8~15cm branches long are taken as cuttings, be soaked in water cuttings, and carries out disinfection；Cuttings bottom is put into plant growth regulating
Soaked in agent treatment fluid, then cuttage is in mixed-matrix；
3) final management and maintenance is carried out, is watered in good time and is sterilized.
Preferably, step 1）Described in right cylinder fruit tree elite stand be wild right cylinder fruit tree, its diameter of a cross-section of a tree trunk 1.3 meters above the ground is 8.0~11.0
Cm, the height of tree are in 6.5~8.5 m.The right cylinder fruit tree elite stand age of tree of the scope is moderate, healthy and robust growth, is suitable as
It is elite stand.
Preferably, step 1）Described in the lignifying branch of cuttings material be wood that lower end footpath is 1.2 cm~1.8cm
Matter branch.
Preferably, step 2）In from place interception 10~12 cm branches long apart from the cm of lignifying shoot apex 8~55
Bar is used as cuttings.
Preferably, step 2）In from place interception 10~12 cm branches long apart from the cm of lignifying shoot apex 12~50
Bar is used as cuttings.
In general, the position of lignifying middle part branch interception cuttings rearward when to obtain cuttings thicker, contained by cuttings branch
Nutritious, under equal conditions, its rootage duration can relatively more a little later, but rooting rate and survival rate can be more preferably.
Present invention research shows that lignifying branch cutting is better than spray effect, and it is probably lignifying shoot vegetative to trace it to its cause
More fully, more nutriments, and tannin can be provided for root growth（The growth of root can be suppressed）Content is also lower.
Preferably, the otch of cuttings is flat mouth.
Present invention research finds that the cutting experiment that upper and lower end flat mouth is cut is cut better than angle, and angle is compared through investigating its rooting rate
What is processed is high by about 10%, and this has broken conventional angle and has cut rooting rate experience high.
Preferably, the cuttings retains 3~4 buds；Retain at least 2 blades near top, every leaf member-retaining portion
Leaf area.
Preferably, step 2）Described in cuttings preparation month be 3~July or 9~October.Because right cylinder fruit tree is heat
Band seeds, are primarily adapted for use in torrid areas plantation.When winter and early spring, weather is too cold, and midsummer August is too hot while being right cylinder
The full-bloom stage of fruit tree, is all unfavorable for cuttage.
Preferably, step 2）Described in preparation month of cuttings be April or September part.
Preferably, step 2）In be soaked in water cuttings 2~4 hours.
The cuttings that is soaked in water can not only make cuttings suction moisture, moreover it is possible to the inhibiting substances degraded in cuttings such as tannin.Pass through
Above-mentioned treatment can further improve cutting plantation and survival rate.
Preferably, step 3）Middle plant growth regulator treatment liquid is ABT rooting powder solutions, and concentration is 80~120 ppm,
Process time is 3~5 h.
Currently preferred, ABT rooting powder solutions used are No. 1 solution of ABT root-inducing powders.
The experiment of different growth regulator treatment shows that ABT root-inducing powders 1 can preferably promote cutting wound healing, morning
Take root, improve rooting of cuttings survival rate.After being processed through No. 1 100 ppm solution of ABT root-inducing powders, the rooting rate of branch compares respectively
The treatment of NAA100 ppm solution and the treatment of IBA100 ppm solution high 1.2%~5% and 2.6%-6%.
Preferably, step 2）Component comprising following percent by volume in middle mixed-matrix：55~85%+pearl of peat soil
20~30%+vermiculite of rock 4~6%.
Preferably, step 2) in mixed-matrix is placed in 50 cave nursery of 540 mm long, 280 mm wide, deep 110 mm specifications
Disk, drench filling and sterilizes, with mixing of drenching for 2 days before cuttage with the liquor potassic permanganate that mass percent is 0.5% to mixed-matrix
Matrix is defined.
There are neat seedling, easily Miao Zhuan, well developed root system, transplanting etc. with the benefit of seedlings nursing plate, and can reduces cost.
Preferably, step 2）Straight cutting method is used during cuttage, per 1 plant of cuttings of cave cuttage.
Preferably, step 3）It is 0.1~0.3% carbendazim solution and/or quality percentage that middle sterilization uses mass percentage concentration
Concentration for 0.1~0.5% liquor potassic permanganate.
The beneficial effects of the invention are as follows：
(1) right cylinder fruit tree cuttage and seedling culture is carried out using the inventive method, breaches right cylinder fruit tree branch because tannin content is high
And technical bottleneck difficult to take root, start to take root within 30~35 days after the branch cutting of its lignifying middle part, can transplant within 4~5 months,
Effectively shorten the required time of taking root, hence it is evident that improve rooting rate and transplanting success, the cuttings life of right cylinder fruit tree cuttage
Root quality is good；Merit can be kept, it is reliable and stable, beneficial to popularization, with broader development prospect.
(2) present invention substitutes the cuttage that angle is cut using the cuttage that upper and lower end flat mouth is cut, and its rooting rate is processed than flat mouth
High by about 10%, this has broken conventional angle and has cut rooting rate experience high.
(3) the inventive method steeps cuttings, preferably ABT rooting powder solutions, concentration using plant growth regulator treatment immersion
It is 80~120 ppm, process time is 3~5 h.The experiment of different growth regulator treatment shows that ABT root-inducing powders 1 can be more
Promotion cutting wound healing well, morning take root, and improve rooting of cuttings survival rate.Processed through No. 1 100 ppm solution of ABT root-inducing powders
Afterwards, the rooting rate of branch is respectively than the treatment of NAA100 ppm solution and that IBA100 ppm solution is processed by high 1.2% and 2.6%.
(4) present invention is obtained and low cost with the common peat soil of in the market, perlite, vermiculite as cutting medium, easily
Honest and clean, saving managerial work cost.
(5) seed plate saving matrix selected by, save space, root system group together, effectively improves transplanting success after seedling；Can
Recycling, saves great amount of cost.
(6) environment required for cuttage is easily realized and convenient management.
Specific embodiment
The raw material sources used in embodiment：
ABT root-inducing powders 1 are the production of Beijing Ai Bidi bio tech ltd；Absolute ethyl alcohol is made a living work bioengineering（Shanghai）
Limited company produces；50% carbendazol wettable powder is produced for Sichuan Guoguang Agrochemical Co., Ltd..
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention not limited to this.
A kind of method of the cuttage and seedling culture of the lignifying middle part branch of the right cylinder fruit tree of embodiment 1（With shoot cuttage
Nursery is contrasted）
Right cylinder fruit tree cuttage operation is carried out in late April, and place is the glass solarium of Chinese forest-science academy's Tropical Forest research institute
It is interior.
(1) preparation of fringe seedling：Selection is healthy and trunk leads to straight wild right cylinder fruit tree select tree (cm of the diameter of a cross-section of a tree trunk 1.3 meters above the ground 8.6, the height of tree
8.0 m), and it is the lignifying branch of 1.6 cm as the source of cuttings material to remove end footpath；
(2) preparation of mixed-matrix is selected with container：By percent by volume for 70%+perlite of peat soil, 25%+vermiculite 5% fills
Divide well mixed, obtain mixed-matrix；Then mixed-matrix is loaded into cuttage vessel, is with mass percent within 2 days before cuttage
0.5% liquor potassic permanganate to matrix drench and fills sterilization, and to drench, matrix is defined, and cuttage vessel is using 540 mm long, width
The 50 cave seedlings nursing plates of 280 mm, deep 110 mm specifications；
(3) preparation and cuttage of cuttings：In the last ten-days period in April, select the morning 7：30th, when illumination is less than 10000 lx, takes step (1) and obtain
The lignifying branch for arriving, the position of the cm of selected distance shoot apex 8 is simultaneously cut into 10 cm cuttings long, is retained near top
2 blades, every leaf retains 2/5 leaf area；Cuttings upper cut is flat mouth, positioned at bud top 0.5cm, is dissipated with reducing moisture
Lose, inferior end notch is flat mouth, positioned at bud lower section 1.0cm, moisture is absorbed beneficial to cuttings；ABT root-inducing powders 1 is molten with absolute ethyl alcohol
Solution, is then configured to No. 1 solution of ABT root-inducing powders of 100 ppm；The cuttings that will be prepared is soaked 3 hours in being put into distilled water, is taken
To go out to be put into sterilize in the liquor potassic permanganate that mass percent is 0.1% clean with distilled water flushing after 1 min；Then by cuttings
Taken out in No. 1 solution of ABT root-inducing powders for 100 ppm that bottom immersion is prepared, after 3 h and treat cuttage.Using straight cutting method, per cave skewer
Insert 1 plant of cuttings；A guiding hole is first made a call in matrix with glass bar before cuttage, depth is 3-4 cm, by its surrounding substrate after inserting
Slightly it is compacted, aforesaid operations are carried out in glass solarium；
(4) final-period management：After cuttage is finished, pour once permeable, spray 50% carbendazol wettable powder, 200 times of liquid comprehensively immediately
Carry out disinfection；Lid plastic sheeting shed on seedbed, keeps cuttage ambient humidity；Most of high light is by shading screen in glass solarium
Filter, but still need to be shaded with the shading screen that light transmittance is 35% at sun-drenched noon, in case cuttings leaf transpiration mistake
Greatly, cuttings dehydration and dry up；After cuttage, every 3 days, sprinkling mass percent was 0.2% liquor potassic permanganate and more than 50% respectively
Bacterium spirit 400 times of liquid of wettable powder carry out alternately sterilization；After 20 days, still alternately sterilized by this concentration, 7 days 1 time；Daily simultaneously
Water spray is carried out, keeps cuttage environment temperature at 25-30 DEG C, humidity is more than 80%.
The 30th day after cuttage starts to take root, and reaches within 50-90 days peak period of taking root, and can transplant within 4-5 month, effectively shortens
The time required to taking root.Compared with shoot cuttage and seedling culture, the rooting rate of embodiment 1 is up to 43.5%, and transplanting success is up to
88.7%, hence it is evident that improve the rooting rate and transplanting success of right cylinder fruit tree cuttage.
Embodiment 2
Right cylinder fruit tree cuttage operation is carried out in early September, and place is the glass solarium of Chinese forest-science academy's Tropical Forest research institute
It is interior.
(1) preparation of fringe seedling：Selection is healthy and trunk leads to straight wild right cylinder fruit tree select tree (cm of the diameter of a cross-section of a tree trunk 1.3 meters above the ground 10, the height of tree
8.5m), it is the lignifying branch of 1.2 cm as the source of cuttings material to remove end footpath；
(2) preparation of mixed-matrix is selected with container：65%+perlite of peat soil, 30%+vermiculite 5% is sufficiently mixed uniformly, is obtained
To mixed-matrix；Then mixed-matrix is loaded into cuttage vessel, before cuttage 2 days it is molten with the potassium permanganate that mass percent is 0.5%
Liquid to matrix drench and fills sterilization, and to drench, mixed-matrix is defined, and cuttage vessel is using 540 mm long, 280 mm wide, deep 500
The cave seed plate of mm specifications 50；
(3) preparation and cuttage of cuttings：In September part the first tenday period of a month, select the morning 9：00th, when illumination is less than 10000 lx, takes step (1) and obtain
The lignifying branch for arriving, the position of the cm of selected distance lignifying shoot apex 18 is simultaneously cut into 10 cm cuttings long, is retained
Near 2 blades at top, every leaf retains 2/5 leaf area；Cuttings upper cut is flat mouth, positioned at 0.5 cm of bud top, to subtract
Few moisture loss, inferior end notch is flat mouth, positioned at 1.0 cm of bud lower section, moisture is absorbed beneficial to cuttings；By ABT root-inducing powders 1 with
A small amount of anhydrous alcohol solution, is then configured to No. 1 solution of ABT root-inducing powders of 100 ppm with distilled water；The cuttings that will be prepared is put
Enter in distilled water and soak 2.5 hours, taking-up is put into the liquor potassic permanganate that mass percent is 0.2% after 1 min that sterilizes with steaming
Distilled water is rinsed well, in No. 1 solution of ABT root-inducing powders for 100 ppm for then preparing the immersion of cuttings bottom, is taken out after 3 h
Treat cuttage；Using straight cutting method, per 1 plant of cuttings of cave cuttage；A guiding hole is first made a call in matrix with glass bar before cuttage, depth is
, slightly be compacted its surrounding substrate after inserting by 3-4 cm, and aforesaid operations are carried out in glass solarium；
(4) final-period management：After cuttage is finished, pour once permeable, spray 50% carbendazol wettable powder, 200 times of liquid comprehensively immediately
Carry out disinfection；Plastic sheeting shed is put up on seedbed, cuttage ambient humidity is kept；Most of high light is shaded in glass solarium
Net is filtered, but still needs to be shaded with the shading screen that light transmittance is 35% at sun-drenched noon, in case cuttings leaf transpiration mistake
Greatly, cuttings dehydration and dry up.After cuttage, every 2 days, sprinkling mass percent was 0.3% liquor potassic permanganate and more than 50% respectively
Bacterium spirit 250 times of liquid of wettable powder carry out alternately sterilization；After 20 days, still alternately sterilized by this concentration, 7 days 1 time；Daily simultaneously
Water spray is carried out, cuttage environment temperature is kept in 25-30 DEG C, humidity 80-90%.
The 34th day after cuttage starts to take root, and reaches within 50~90 days peak period of taking root, and can transplant within 4-5 month, effectively contracting
It is short take root the time required to.Rooting rate is up to 47.0%, and transplanting success is up to 90.5%, hence it is evident that improve right cylinder fruit tree cuttage
Rooting rate and transplanting success.
Embodiment 3
Right cylinder fruit tree cuttage operation is carried out in April, and place is in the glass web room of Chinese forest-science academy's Tropical Forest research institute.
(1) preparation of fringe seedling：Selection is healthy and trunk leads to straight wild right cylinder fruit tree select tree (cm of the diameter of a cross-section of a tree trunk 1.3 meters above the ground 8, the height of tree 6.7
M), it is the lignifying branch of 1.8 cm as the source of cuttings material to remove end footpath；
(2) preparation of mixed-matrix is selected with container：74%+perlite of peat soil, 22%+vermiculite 4% is sufficiently mixed uniformly, is obtained
To mixed-matrix；Then mixed-matrix is loaded into cuttage vessel, before cuttage 2 days it is molten with the potassium permanganate that mass percent is 0.5%
Liquid to matrix drench and fills sterilization, and to drench, mixed-matrix is defined, and cuttage vessel is using 540 mm long, 280 mm wide, deep 500
50 cave seedlings nursing plates of mm specifications；
(3) preparation and cuttage of cuttings：Late May, select the morning 8：00th, when illumination is less than 10000 lx, takes step (1) and obtain
Lignifying branch, the position of the cm of selected distance lignifying shoot apex 28 and to be cut into 10 cm long retains near top
2 blades, every leaf retains 2/5 leaf area；Cuttings upper cut is flat mouth, positioned at 0.5 cm of bud top, is dissipated with reducing moisture
Lose, inferior end notch is flat mouth, positioned at 1.0 cm of bud lower section, moisture is absorbed beneficial to cuttings；By ABT root-inducing powders 1 with a small amount of anhydrous
Ethanol is dissolved, and No. 1 solution of ABT root-inducing powders of 100 ppm is then configured to distilled water；The cuttings that will be prepared is put into distilled water
Middle immersion 2 hours, taking-up is put into the liquor potassic permanganate that mass percent is 0.2% dry with distilled water flushing after 2 min that sterilize
Only, taken out in No. 1 solution of ABT root-inducing powders for 120 ppm for then preparing the immersion of cuttings bottom, after 3 h and treat cuttage：Using
Straight cutting method, per 1 plant of cuttings of cave cuttage；A guiding hole is first made a call in matrix with glass bar before cuttage, depth is 3-4 cm, after inserting
Its surrounding substrate is slightly compacted, aforesaid operations are carried out in glass solarium；
(4) final-period management：After cuttage is finished, pour once permeable, spray 50% carbendazol wettable powder, 200 times of liquid comprehensively immediately
Carry out disinfection；Plastic sheeting shed is put up on seedbed, cuttage ambient humidity is kept.Most of high light is shaded in glass solarium
Net is filtered, but still needs to be shaded with the shading screen that light transmittance is 35% at sun-drenched noon, in case cuttings leaf transpiration mistake
Greatly, cuttings dehydration and dry up；After cuttage, every 2 days, sprinkling mass percent was 0.2% liquor potassic permanganate and more than 50% respectively
Bacterium spirit 400 times of liquid of wettable powder carry out alternately sterilization；After 20 days, still alternately sterilized by this concentration, 7 days 1 time；Daily simultaneously
Water spray is carried out, keeps cuttage environment temperature at 20-25 DEG C, humidity is more than 85%.
The 32nd day after cuttage starts to take root, and reaches within 65~90 days peak period of taking root, and can transplant within 4-5 month, shortens
The time required to taking root.Rooting rate is up to 46.4%, and transplanting success is up to 89.6%, improves the rooting rate of right cylinder fruit tree cuttage
And transplanting success.
The present invention is obtained and with low cost with common peat soil, perlite, vermiculite as cutting medium, easily；Cuttage is held
Device is repeatable to be utilized, and saves great amount of cost.The cuttage of right cylinder fruit tree is carried out by this method, starts within 30-35 days to take root after inserting,
Can transplant within 4-5 month, effectively shorten the time required to taking root, hence it is evident that improve rooting rate and the transplanting of right cylinder fruit tree cuttage
Survival rate, cuttage rooting quality are good；Merit can be kept, it is reliable and stable, it is convenient to promote, with vast potential for future development.
Comparative example 1
Right cylinder fruit tree cuttage operation is carried out in early January, and place is in Chinese forest-science academy Tropical Forest research institute glass web room.
(1) preparation of fringe seedling：Collection is healthy and trunk leads to straight wild right cylinder fruit tree select tree (cm of the diameter of a cross-section of a tree trunk 1.3 meters above the ground 8.6, the height of tree
8.0 lignifying branch m) is used as cuttings material；
(2) preparation of mixed-matrix is selected with container：70%+perlite of peat soil, 25%+vermiculite 5% is sufficiently mixed uniformly, is obtained
To mixed-matrix；Then mixed-matrix is loaded into cuttage vessel, before cuttage 2 days it is molten with the potassium permanganate that mass percent is 0.5%
Liquid to matrix drench and fills sterilization, and to drench, matrix is defined, and cuttage vessel is using 540 mm long, 280 mm wide, deep 500 mm rule
50 cave seedlings nursing plates of lattice；
(3) preparation and cuttage of cuttings：Early January, select the morning 8：00th, when illumination is less than 10000 lx, takes step (1) and obtain
Semi-lignified spray, the position of the cm of selected distance semi-lignified spray top 8 and to be cut into 12 cm long retains near top
2 blades in portion, every leaf retains 2/5 leaf area；Cuttings upper cut is flat mouth, positioned at bud top 1cm, is dissipated with reducing moisture
Lose, inferior end notch is flat mouth, positioned at bud lower section 2cm, moisture is absorbed beneficial to cuttings；ABT root-inducing powders 1 is molten with absolute ethyl alcohol
Solution, is then configured to No. 1 solution of ABT root-inducing powders of 100 ppm；The cuttings that will be prepared is soaked 2 hours in being put into distilled water, is taken
To go out to be put into sterilize in the liquor potassic permanganate that mass percent is 0.2% it is clean with distilled water flushing after 2 min, then by cuttings
Taken out in No. 1 solution of ABT root-inducing powders for 100 ppm that bottom immersion is prepared, after 3 h and treat cuttage：Using straight cutting method, per cave skewer
Insert 1 plant of cuttings；A guiding hole is first made a call in matrix with bamboo cane before cuttage, depth is 3-4 cm, after inserting by its surrounding substrate slightly
Pressurization is real, and aforesaid operations are carried out in glass solarium；
(4) final-period management：After cuttage is finished, pour once permeable, spray 50% carbendazol wettable powder, 200 times of liquid comprehensively immediately
Carry out disinfection；Plastic sheeting shed is put up on seedbed, cuttage ambient humidity is kept.Most of high light is shaded in glass solarium
Net is filtered, but still needs to be shaded with the shading screen that light transmittance is 35% at sun-drenched noon, in case cuttings leaf transpiration mistake
Greatly, cuttings dehydration and dry up；After cuttage, every 2 days, sprinkling mass percent was 0.2% liquor potassic permanganate and more than 50% respectively
Bacterium spirit 400 times of liquid of wettable powder carry out alternately sterilization；After 20 days, still alternately sterilized by this concentration, 7 days 1 time；Daily simultaneously
Water spray is carried out, cuttage environment temperature is kept in 25-30 DEG C, humidity 80~90%.
The 42nd day after cuttage starts to take root, and reaches within 75~100 days peak period of taking root, and can transplant within 4-5 month.Rooting rate
18.9%, transplanting success 85.2%, the rooting rate of right cylinder fruit tree cuttage is significantly lower than preferred month.Reason is, early spring skewer
Insert, because soil temperature is not enough and caused by take root difficulty.
Comparative example 2
Right cylinder fruit tree cuttage operation is carried out in early August, and place is in Chinese forest-science academy Tropical Forest research institute glass web room.
(1) preparation of fringe seedling：Collection is healthy and trunk leads to straight wild right cylinder fruit tree select tree (cm of the diameter of a cross-section of a tree trunk 1.3 meters above the ground 8.6, the height of tree
8.0 lignifying branch m) is used as cuttings material；
(2) preparation of mixed-matrix is selected with container：70%+perlite of peat soil, 25%+vermiculite 5% is sufficiently mixed uniformly, is obtained
To mixed-matrix；Then mixed-matrix is loaded into cuttage vessel, before cuttage 2 days it is molten with the potassium permanganate that mass percent is 0.5%
Liquid to matrix drench and fills sterilization, and to drench, mixed-matrix is defined, and cuttage vessel is using 540 mm long, 280 mm wide, deep 500
50 cave seedlings nursing plates of mm specifications；
(3) preparation and cuttage of cuttings：Early August, select the morning 8：00th, when illumination is less than 10000 lx, takes step (1) and obtain
Lignifying branch, the position of the cm of selected distance lignifying shoot apex 8 is simultaneously cut into that 10 cm are long, is retained near top
2 blades, every leaf retains 2/5 leaf area；Cuttings upper cut is flat mouth, positioned at bud top 1cm, to reduce moisture loss, under
End otch is flat mouth, positioned at bud lower section 2cm, moisture is absorbed beneficial to cuttings；By the anhydrous alcohol solution of ABT root-inducing powders 1, then
It is configured to No. 1 solution of ABT root-inducing powders of 100 ppm；The cuttings that step (3) is prepared is put into distilled water and is soaked 2 hours, taken
To go out to be put into sterilize in the liquor potassic permanganate that mass percent is 0.2% it is clean with distilled water flushing after 2 min, then by cuttings
Taken out in No. 1 solution of ABT root-inducing powders for 100 ppm that bottom immersion is prepared, after 3 h and treat cuttage：Using straight cutting method, per cave skewer
Insert 1 plant of cuttings；A guiding hole is first made a call in matrix with bamboo cane before cuttage, depth is 3-4 cm, after inserting by its surrounding substrate slightly
Pressurization is real, and aforesaid operations are carried out in glass solarium；
(4) final-period management：After cuttage is finished, pour once permeable, spray 50% carbendazol wettable powder, 200 times of liquid comprehensively immediately
Carry out disinfection；Plastic sheeting shed is put up on seedbed, cuttage ambient humidity is kept.Most of high light is shaded in glass solarium
Net is filtered, but still needs to be shaded with the shading screen that light transmittance is 35% at sun-drenched noon, in case cuttings leaf transpiration mistake
Greatly, cuttings dehydration and dry up；After cuttage, every 2 days, sprinkling mass percent was 0.1% liquor potassic permanganate and more than 50% respectively
Bacterium spirit 400 times of liquid of wettable powder carry out alternately sterilization；After 20 days, still alternately sterilized by this concentration, 7 days 1 time；Daily simultaneously
Water spray is carried out, cuttage environment temperature is kept in 25-30 DEG C, humidity 80~90%.
The 38th day after cuttage starts to take root, and reaches within 75~100 days peak period of taking root, and can transplant within 4-5 month.Rooting rate is
23.0%, transplanting success is 78.6%, and the rooting rate of right cylinder fruit tree cuttage is significantly lower than preferred month.
Cuttage month September and April be most preferred.And August is the full-bloom stage of right cylinder fruit tree, shoot vegetative is a large amount of
It is consumed in and blooms, is unfavorable for cuttage.
Comparative example 3
, with embodiment 1, difference is that cuttage is shoot, and the cuttings for preparing in addition is not put into distilled water for other
Immersion, directly sterilizes and is put into immersion in plant growth regulator treatment liquid.
The 36th day after cuttage starts to take root, and reaches within 65~100 days peak period of taking root, and can transplant within 4-5 month.Rooting rate
32.5%, transplanting success 80.2%, it is seen then that the rooting rate of right cylinder fruit tree epicormic branch cuttage is significantly lower than lignifying branch.
Comparative example 4
, with embodiment 1, difference is to replace No. 1 100 ppm solution of ABT root-inducing powders to inserting with IBA100 ppm solution for other
Fringe is processed.
The 33rd day after cuttage starts to take root, and reaches within 60-95 days peak period of taking root, and can transplant within 4-5 month, and rooting rate reaches
40.9%, transplanting success is up to 82.5%, less than ABT root-inducing powders No. 1 rooting rate and transplanting success for the treatment of.
Comparative example 5
, with embodiment 1, difference is that the otch of lignifying branch cuttings is conventional angle for other（Including upper cut with
Otch）.Result is：The 35th day after cuttage starts to take root, and reaches within 58-95 days peak period of taking root, and can transplant within 4-5 month, rooting rate
Up to 38.2%, transplanting success up to 81.0%, less than the rooting rate and transplanting success of flat mouth cuttings.
Above-described embodiment is the present invention preferably implementation method, but embodiments of the present invention are not by above-described embodiment
Limitation, other any ends away from Spirit Essence of the invention and the change, modification, replacement made under principle, combine, it is simplified,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of cuttage and seedling culture method of right cylinder fruit tree, it is characterised in that comprise the following steps：
1）The preparation of cuttings：Select health and trunk is logical straight, right cylinder of the diameter of a cross-section of a tree trunk 1.3 meters above the ground not less than 8.0 cm, the height of tree not less than 6.5m is really
Wood is elite stand, using lignifying branch that lower end footpath is 1.0 cm~2.0 cm as cuttings material；
2）The preparation and cuttage of cuttings：By step 1) the lignifying branch that obtains, cuts from apart from the place of 6~60cm of its top
8~15cm branches long are taken as cuttings, be soaked in water cuttings, and carries out disinfection；Cuttings bottom is put into plant growth regulating
Soaked in agent treatment fluid, then cuttage is in mixed-matrix；
3）Final management and maintenance is carried out, is watered in good time and is sterilized.
2. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 1）Described in right cylinder fruit tree elite stand be
Wild right cylinder fruit tree, its diameter of a cross-section of a tree trunk 1.3 meters above the ground is in 8.0~11.0 cm, the height of tree in 6.5~8.5 m.
3. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 1）Described in cuttings material it is wooden
It is that lower end footpath is the lignifying branch of 1.2cm~1.8 cm to change branch.
4. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 2）In from apart from lignifying shoot apex
The place of 8~55 cm intercepts 10~12 cm branches long as cuttings.
5. cuttage and seedling culture method according to claim 4, it is characterised in that：The otch of cuttings is flat mouth.
6. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 2）Described in preparation month of cuttings be
3~July or 9~October.
7. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 2）In the cuttings 2~4 that is soaked in water it is small
When.
8. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 3）Middle plant growth regulator treatment liquid
It is ABT rooting powder solutions, concentration is 80~120 ppm, and process time is 3~5 h.
9. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 2）Following body is included in middle mixed-matrix
The component of product percentage：Peat soil 55~85%, perlite 20~30% and vermiculite 4~6%.
10. cuttage and seedling culture method according to claim 1, it is characterised in that：Step 3）Middle sterilization is dense using quality percentage
Spend for liquor potassic permanganate that 0.1~0.3% carbendazim solution and/or mass percentage concentration are 0.1~0.5%.
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