CN106701553A - Small size shaking flask fermentation equipment - Google Patents
Small size shaking flask fermentation equipment Download PDFInfo
- Publication number
- CN106701553A CN106701553A CN201710106262.XA CN201710106262A CN106701553A CN 106701553 A CN106701553 A CN 106701553A CN 201710106262 A CN201710106262 A CN 201710106262A CN 106701553 A CN106701553 A CN 106701553A
- Authority
- CN
- China
- Prior art keywords
- main body
- equipment main
- zymolysis equipment
- fermentation equipment
- openend
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title abstract description 18
- 230000004151 fermentation Effects 0.000 title abstract description 18
- 238000007789 sealing Methods 0.000 abstract description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 abstract description 3
- 239000001301 oxygen Substances 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000010354 integration Effects 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 238000005070 sampling Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000011021 bench scale process Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000011169 microbiological contamination Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention specially discloses small size shaking flask fermentation equipment which comprises a fermentation equipment main body, wherein the fermentation equipment main body is in a columnar shape; an opening is formed at one end of the fermentation equipment main body; screw threads are formed inside and outside the opening end; a sealed bottom is arranged at the other end of the fermentation equipment main body; the opening end is movably connected with a columnar centrifugal tube through the screw threads; an external screw thread is formed at an opening end of the columnar centrifugal tube; one or more of columnar tubes or square tubes which are vertical to the fermentation equipment main body or are obliquely inserted into the fermentation equipment main body is/are arranged at a columnar part of the fermentation equipment main body; and the columnar tubes or the square tubes are in seamless integration with the fermentation equipment main body to form a connection body. The small size shaking flask fermentation equipment has the advantages that complex sterile operations such as sample taking, sample adding, even medium replacement and the like can be conveniently and fast realized; by selecting different sizes of the lateral openings of the fermentation equipment main body, edges of the top ends of the openings and flask sealing membranes, the oxygen supply quantity in a cultivation process can be adjusted, so as to meet different cultivation conditions of anaerobic cultivation, facultative anaerobic cultivation and aerobiotic cultivation.
Description
Technical field
The present invention relates to Zymolysis Equipment technical field, a kind of small flasks Zymolysis Equipment is specifically disclosed.
Background technology
Determination and optimization of expression quantity screening, bacterial strain inducing expression condition optimization and zymotechnique in engineered strain etc.
In experimental exploration, the small-sized fermentation experiment of laboratory scale is all indispensable.At present, these experiments are shaken by conical flask
Bottle culture is completed, if some experiments are needed when culture medium is changed during strain culturing, with shaking flask and centrifuge tube
Realize.This there is problems with：First, be limited by size, the size of shaking table of shaking flask so that experiment cannot high flux carry out；
2nd, the carrying out that sampling, sample-adding in culture etc. cannot quickly, easy；If the 3, needing to change culture medium in culture, then
Aseptic centrifuge tube must be coordinated to complete, operating procedure is more, increase the possibility of pollution；4th, in incubation, it is impossible to main
See the supply mode and quantity delivered of regulation oxygen.
The content of the invention
It is an object of the invention to provide a kind of small flasks Zymolysis Equipment, its is easy to operate, and intact meets laboratory
Whole demands of bench scale fermentation test.
To achieve these goals, present invention employs following technical scheme：A kind of small flasks Zymolysis Equipment, including
Zymolysis Equipment main body, the Zymolysis Equipment main body is in the form of a column shape, and described Zymolysis Equipment main body one end is opening, the openend
Inside and outside to be equipped with screw thread, the other end is sealing bottom, and the openend is flexibly connected a column centrifuge tube, the post by screw thread
Shape centrifuge tube openend is provided with external screw thread, the stylolitic part of the Zymolysis Equipment main body be provided with one or more vertical or oblique cuttings in
The stylostome or rectangular tube of the Zymolysis Equipment main body, the stylostome or rectangular tube are with the Zymolysis Equipment main body by seamless
It is bonded a connected component.
Further, dismountable pinched valve is provided with the Zymolysis Equipment main body, circle is provided with inside the pinched valve
Shape hole or semi-circular hole, outside side are provided with intermediate plate, and it could be sandwiched in whole shake flask fermentation equipment anywhere.
Further, the Zymolysis Equipment body openings end is provided with the first closure, and first closure passes through spiral shell
Line rotary moveable is connected to the openend of the Zymolysis Equipment main body.
Further, the column centrifuge tube openend is provided with the second closure, and second closure passes through screw thread
Rotary moveable is connected to the openend of the column centrifuge tube, and the column centrifuge tube other end is the sealing at round bottom or sharp bottom
Bottom.
Further, the sealing bottom of the Zymolysis Equipment main body can be round bottom, sharp bottom or flat.
Further, the stylostome top end opening is round or broadening formation or square.
The beneficial effects of the present invention are：Can by Zymolysis Equipment main body, the first closure and being used in combination for pinched valve
To realize aerobic condition culture；Anaerobism bar can be realized by being used in combination for column centrifuge tube, the second closure and pinched valve
Part culture；And by being used in combination for component Zymolysis Equipment main body, column centrifuge tube and pinched valve, have the advantage that：
1st, on the premise of laboratory hardware condition is not changed, the bearing capacity of shaking table is improve, can be realized under the conditions of
High throughput test；
2nd, due to easy to operate, can preferably complete Large-scale Screening, single factor test and it is multifactor it is orthogonal etc. need it is big
The experiment that amount is repeated；
3rd, can easily realize sampling, be loaded, even change the complex operations such as culture medium；Especially changing culture
During base, existing test method is cumbersome, easily microbiological contamination, and present device can coordinate existing laboratory common apparatus
Under the premise of, experiment demand is realized in perfection；
4th, can by Zymolysis Equipment main body side opening, open top end edge and envelope bottle film different size selection,
The quantity delivered of oxygen in regulation incubation, meets anaerobism, amphimicrobian and the different condition of culture such as aerobic.
Brief description of the drawings
Technical scheme in order to illustrate more clearly the embodiments of the present invention, embodiment will be described below needed for be used
Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are only the present invention in order to illustrate more clearly of this hair
Bright embodiment or technical scheme of the prior art, it is attached by what is used needed for p- embodiment or description of the prior art below
Figure is briefly described, for those of ordinary skill in the art, on the premise of not paying creative work, can be with root
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is a kind of small flasks Zymolysis Equipment structural representation of the invention.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
A kind of small flasks Zymolysis Equipment as shown in Figure 1, including Zymolysis Equipment main body 1, the Zymolysis Equipment main body 1 is in
Column shape, described one end of Zymolysis Equipment main body 1 is opening, and the openend is inside and outside to be equipped with screw thread, and the other end is sealing bottom,
The sealing bottom can be round bottom, sharp bottom or flat；The openend is flexibly connected a column centrifuge tube 3 by screw thread, described
The openend of column centrifuge tube 3 is provided with external screw thread；It is vertical or oblique that the stylolitic part of the Zymolysis Equipment main body 1 is provided with one or more
The stylostome or rectangular tube of the Zymolysis Equipment main body are inserted in, the stylostome or rectangular tube are logical with the Zymolysis Equipment main body 1
Cross seamless combination and constitute a connected component, the stylostome top end opening is round or broadening formation or square.
Dismountable pinched valve 5 is provided with the Zymolysis Equipment main body 1, be provided with inside the pinched valve 5 circular port or
Semi-circular hole, outside side is provided with intermediate plate, by compressing the intermediate plate of side, can cause that it opens from centre, waits to be put into properly
Position at unclamp, you can be arbitrarily clipped in whole shake flask fermentation equipment anywhere.
Wherein, the openend of Zymolysis Equipment main body 1 is provided with the first closure 2, and first closure 2 passes through screw thread
Rotary moveable is connected to the openend of the Zymolysis Equipment main body 1.
Wherein, the openend of column centrifuge tube 3 is provided with the second closure 4, and second closure 4 is revolved by screw thread
Turn to be movably connected on the openend of the column centrifuge tube 3, and the other end of column centrifuge tube 3 is the sealing at round bottom or sharp bottom
Bottom.
When using preceding sterilizing：First closure 2 is rotatably connected in the Zymolysis Equipment main body 1, Zymolysis Equipment master
Stylostome or square tube opening on body 1 are wrapped up with the conventional envelope bottleneck method in laboratory；By the second closure 4 be rotatably connected in
On the column centrifuge tube 3.
When using：The first closure of rotating separation 2 and Zymolysis Equipment main body 1, the second closure 4 and column centrifuge tube 3,
Appropriate culture medium and strain is added in column centrifuge tube 3, then Zymolysis Equipment main body 1 is rotatably connected in column centrifuge tube 3
On, then pinched valve 5 is clipped in the appropriate position of integral device, the whole shake flask fermentation equipment that will finally connect laterally (ensures
The side stylostome or rectangular tube opening upwards of Zymolysis Equipment main body 1) it is placed in shaking table, normal culture.
Also first the Zymolysis Equipment main body 1 that the side stylostome or square tube opening of Zymolysis Equipment main body 1 are wrapped can be revolved
Turn to be connected on column centrifuge tube 3, after overall sterilizing, pressed from the upside flat column pipe of Zymolysis Equipment main body 1 or square tube opening
Routine test operating method adds appropriate culture medium and strain, then pinched valve 5 is pressed from both sides in position, laterally (ensures hair
The side stylostome or rectangular tube opening upwards of ferment equipment body 1) it is placed in shaking table, normal culture.
When sampling or fluid infusion：Whole shake flask fermentation equipment is taken out from shaking table, from the side column of Zymolysis Equipment main body 1
At pipe or square tube opening, the routinely operation of shaking flask sampling or fluid infusion is carried out.
When changing culture medium：Remove pinched valve 5, whole equipment is disposed vertically (Zymolysis Equipment main body 1 upper, column from
Heart pipe 3 is under), Zymolysis Equipment main body 1 and column centrifuge tube 3 are separated by rotating, then revolved with the second aseptic closure 4
Turn be connected on column centrifuge tube 3, empirically room conventional centrifugal method and require operating assembly column centrifuge tube 3 and second seal
The association of lid 4；After the completion of centrifugation, the second closure 4 is removed in rotation, and empirically room conventional method changes component column centrifuge tube 3
In culture medium, then component Zymolysis Equipment main body 1 is rotatably connected on column centrifuge tube 3, readjusted the position of pinched valve 5
Put, the whole equipment that will be connected is laterally disposed in shaking table, you can continue to cultivate.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (8)
1. a kind of small flasks Zymolysis Equipment, it is characterised in that：Including Zymolysis Equipment main body (1), the Zymolysis Equipment main body (1)
It is in the form of a column shape, Zymolysis Equipment main body (1) one end open, openend is inside and outside to be equipped with screw thread, and the other end is sealing bottom, institute
State openend and one column centrifuge tube (3) is flexibly connected by screw thread, column centrifuge tube (3) openend is provided with external screw thread, institute
The stylolitic part for stating Zymolysis Equipment main body (1) is provided with one or more tubes for being connected to the Zymolysis Equipment main body (1), institute
State tube and a connected component is constituted by seamless combination with the Zymolysis Equipment main body (1).
2. a kind of small flasks Zymolysis Equipment according to claim 1, it is characterised in that：The Zymolysis Equipment main body (1)
On be provided with dismountable pinched valve (5), is provided with circular port or semi-circular hole inside the pinched valve (5), outside side is provided with
Intermediate plate.
3. a kind of small flasks Zymolysis Equipment according to claim 1, it is characterised in that：The Zymolysis Equipment main body (1)
Openend is provided with the first closure (2), and first closure (2) is movably connected on the Zymolysis Equipment by screw thread rotation
The openend of main body (1).
4. a kind of small flasks Zymolysis Equipment according to claim 1, it is characterised in that：The column centrifuge tube (3) is opened
Mouth end is provided with the second closure (4), and second closure (4) is movably connected on the column centrifuge tube by screw thread rotation
(3) openend, and the column centrifuge tube (3) other end is the sealing bottom at round bottom or sharp bottom.
5. a kind of small flasks Zymolysis Equipment according to claim 1, it is characterised in that：The Zymolysis Equipment main body (1)
Sealing bottom can be round bottom, sharp bottom or flat.
6. a kind of small flasks Zymolysis Equipment according to claim 1, it is characterised in that：The tube top end opening is
Round or broadening formation is square.
7. a kind of small flasks Zymolysis Equipment according to claim 1, it is characterised in that：The tube be stylostome or
Rectangular tube.
8. a kind of small flasks Zymolysis Equipment according to claim 1, it is characterised in that：The Zymolysis Equipment main body (1)
Stylolitic part be provided with one or more vertical or oblique cuttings in the tube of the Zymolysis Equipment main body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710106262.XA CN106701553A (en) | 2017-02-27 | 2017-02-27 | Small size shaking flask fermentation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710106262.XA CN106701553A (en) | 2017-02-27 | 2017-02-27 | Small size shaking flask fermentation equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106701553A true CN106701553A (en) | 2017-05-24 |
Family
ID=58917584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710106262.XA Pending CN106701553A (en) | 2017-02-27 | 2017-02-27 | Small size shaking flask fermentation equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106701553A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1935973A1 (en) * | 2006-12-23 | 2008-06-25 | Stiftung Alfred-Wegener-Insitut für Polar und Meeresforschung | Culture device for aquatic organisms |
| CN201940247U (en) * | 2010-11-25 | 2011-08-24 | 陈云校 | Test tube clamp |
| JP3172219U (en) * | 2011-09-27 | 2011-12-08 | 学校法人星薬科大学 | Centrifuge tube |
| CN202220169U (en) * | 2011-10-09 | 2012-05-16 | 刘美芹 | Bacteria culture bottle convenient for sampling |
| CN203393147U (en) * | 2012-12-31 | 2014-01-15 | 北大方正集团有限公司 | Cap for cryogenic vial and cryogenic vial |
| CN204699720U (en) * | 2015-03-27 | 2015-10-14 | 中国石油化工股份有限公司 | A kind of Combined centrifuge tube and Storage Box thereof |
| CN205382160U (en) * | 2016-01-21 | 2016-07-13 | 延安大学 | Fermentation test tube |
-
2017
- 2017-02-27 CN CN201710106262.XA patent/CN106701553A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1935973A1 (en) * | 2006-12-23 | 2008-06-25 | Stiftung Alfred-Wegener-Insitut für Polar und Meeresforschung | Culture device for aquatic organisms |
| CN201940247U (en) * | 2010-11-25 | 2011-08-24 | 陈云校 | Test tube clamp |
| JP3172219U (en) * | 2011-09-27 | 2011-12-08 | 学校法人星薬科大学 | Centrifuge tube |
| CN202220169U (en) * | 2011-10-09 | 2012-05-16 | 刘美芹 | Bacteria culture bottle convenient for sampling |
| CN203393147U (en) * | 2012-12-31 | 2014-01-15 | 北大方正集团有限公司 | Cap for cryogenic vial and cryogenic vial |
| CN204699720U (en) * | 2015-03-27 | 2015-10-14 | 中国石油化工股份有限公司 | A kind of Combined centrifuge tube and Storage Box thereof |
| CN205382160U (en) * | 2016-01-21 | 2016-07-13 | 延安大学 | Fermentation test tube |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |