CN106701322A - Method for extracting fermented morinda citrifolia seed essential oil - Google Patents
Method for extracting fermented morinda citrifolia seed essential oil Download PDFInfo
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- CN106701322A CN106701322A CN201710063422.7A CN201710063422A CN106701322A CN 106701322 A CN106701322 A CN 106701322A CN 201710063422 A CN201710063422 A CN 201710063422A CN 106701322 A CN106701322 A CN 106701322A
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- buddhist nun
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a method for extracting fermented morinda citrifolia seed essential oil. The method comprises the following steps: (1) smashing fermented morinda citrifolia seeds, drying, crushing, and screening; (2) mixing a pretreated fermented morinda citrifolia seed raw material with an ethanol solution, soaking, performing suction filtration so as to obtain supernate, and centrifuging by using a high-speed centrifuge, so as to obtain a supernate liquid without impurities; (3) removing the solvent in the supernate liquid by using a rotation evaporator, and observing and obtaining an extract when no liquid drops down from a condensation tube of the rotation evaporator; and (4) placing the extract for 24 hours at 16 DEG C, thereby obtaining a layered oil object, that is, the fermented morinda citrifolia seed essential oil. As the essential oil is extracted from the waste, that is, fermented morinda citrifolia seeds, generated in the process that yeast is produced from morinda citrifolia, the raw material resource is rich, resource recycling can be achieved, normal temperature extraction is adopted, volatile components can be maintained to the maximum extent, the extraction rate is high, a small amount of solvent residues can be generated, the operation is safe, and the essential oil can be used as a skin-care additive.
Description
Technical field
The invention belongs to plant extracts preparation method technical field, and in particular to extracted in one kind fermentation promise Buddhist nun's fruit seed
The method of essential oil.
Background technology
Nuo Ni is Rubiaceae Morinda, and Nuo Ni (Noni), also known as morinda citrifolia, India's mulberries, is that one kind is grown on the torrid zone
A kind of shrub of perennial evergreen broad-leaved or dungarunga in area, it is high 3-10 meters, there is the leaf of substantial amounts of ellipse (long 5-17 lis
Rice, wide 10-40 centimetres).There is plantation in Hainan Province of China and Taiwan Province some areas.Its root, bark, stem, leaf, fruit have been made
It is that Traditional Folk medicine is used to treat various diseases, such as diabetes, hypertension and cancer.Contain 80% in fresh promise Buddhist nun fruit
Moisture, main dry is soluble solids, dietary fiber and protein.In ripening fruits, it has been determined that volatility
Compound be organic acid, alcohols, esters, ketone and lactone, promise Buddhist nun fruit in also containing carrotene, vitamin C, amino acid and
Sai Nuoning original isoreactivity materials.Nuo Ni fruits belong to evergreen tropical fruit tree, and mellow fruit can be harvested among 1 year.Corolla is blue or green
White promise Buddhist nun fruit (long 3-10 centimetres, wide 3-6 centimetres, ovalize) meat is plump outward appearance has embossment sense.The general fruit plucked
It is actually white.The surface of Nuo Ni fruits is covered by the seed containing bronzing bud.Mellow fruit gives out one strong fourth
Sour stink.After fruit maturation, avette, pulp succulence is shaped as, slightly dark yellow or white, in gel, many of which is red
The hard twist irregularity of brown, each hard twist irregularity is contained within four seeds.Originally green fruit is green, is turned yellow after maturation,
Soften when excessively ripe and be translucent shape and give out butyric acid smell beastly.
In recent years, promise Buddhist nun fruit turns into domestic and international health food industry one kind has protection blood vessel, anti-oxidant, protect liver, anti-mistake
The effects such as quick, antibacterium, antiviral, antifungi, anticancer, strengthen immunity.2004, Chinese scholar found in Xisha Yongxing Island
Chinese promise Buddhist nun fruit germ plasm resource.2005, Hainan Nuo Ni industrial parks development corporation, Ltd. started the artificial culture of promise Buddhist nun and is
Row research and development of products plan, builds up more than 3000 mus of Xisha promise Buddhist nun's demonstration plant base, the Xisha promise of independent research in Hainan at present
Buddhist nun's series of products, market is entered including Xisha promise Buddhist nun ferment, Xisha promise Buddhist nun tea, Xisha promise Buddhist nun's perfumed soap etc. comprehensively.Promise Buddhist nun's fruit
Can produce substantial amounts of discarded object during fermented beverage and non-fermented beverage is made, at present, the product of promise Buddhist nun be mostly with
Pomace after liquid supply market after Nuo Ni fruit fermentations, but promise Buddhist nun fruit fermentation is not yet fully used including seed.At present
The research for extracting essential oil for fermentation promise Buddhist nun fruit seed is also less, has no related patent and document report.
The content of the invention
For the technical scheme that solution above-mentioned technical problem is used is：A kind of extraction fermentation promise Buddhist nun's fruit seed essential oil is provided
Preparation method.Specific technical scheme is as follows：
(1) pre-process：By the promise Buddhist nun's fruit baked seed for fermenting, crush, sieving is continued to employ；
(2) soak：Pretreated fermentation promise Buddhist nun's fruit seed meal is weighed, the ethanol solution with 95% mixes, solid-to-liquid ratio 1:8
~12,4~10h of soak at room temperature；
(3) suction filtration：Soak is obtained into supernatant by Buchner funnel suction filtration；
(4) high speed centrifugation：Supernatant is centrifuged with supercentrifuge, supernatant liquor free from foreign meter is obtained；
(5) rotary evaporation：By supernatant liquor by Rotary Evaporators evaporation solvent, extract is obtained；
(6) high speed centrifugation：The extract high speed centrifugation, 5000~10000r/min of rotating speed, time 3 again of solvent will be removed
~10min, obtains oily mater；
(7) refrigerate：The oily mater that will be obtained is put into refrigerator and stands refrigeration, 0~8 DEG C of temperature, and cold preservation time is 1~10h,
The essential oil layering of fermentation promise Buddhist nun's fruit seed is obtained, upper and lower two-layer oily mater, upper strata is light brown, and lower floor's color is dark brown.
In for above-mentioned technical proposal, the fermentation process of described fermentation promise Buddhist nun's fruit seed is：White promise Buddhist nun's fresh fruit is plucked,
Cleaning, puts into fermentation tank, 20~40 DEG C of anaerobic fermentation temperature control, 100~150d of fermentation time；PH value 6.8-7.2；Using promise
Buddhist nun fruit the endophyte of itself, bacterial strain CICC10883 cocoa beans acetobacter can complete fermentation, take the solid content in tunning,
It is standby.Wherein, the identification of bacterium CICC 10883 used has been studied in the literature with biological characteristics：《1 plant of Xisha promise Buddhist nun
The identification of fruit protoplasm bacterium CICC 10883 and biological characteristics》,《Chinese food journal》8th phase in 2016, Cao Yanhua etc..
In for above-mentioned technical proposal, described drying temperature is that 50~60 DEG C of sievings are 20~100 mesh sieves.
For in above-mentioned technical proposal, the centrifugation described in step (4) is that supernatant is centrifuged into 3 with 5000~10000r/min
~10min.
In for above-mentioned technical proposal, described rotary evaporation is that do not have drop in Rotary Evaporators condenser pipe is observed
Terminate evaporation when dripping.
The method of the present invention is simple, and fermentation promise Buddhist nun's fruit seed essential oil has extremely strong inoxidizability, process safety collar
Protect, can be used as cosmetic additive agent.
Brief description of the drawings
Fig. 1：Technology path schematic diagram；
Fig. 2：Oily mater after ethanol extraction；Wherein：A is obtained for the promise Buddhist nun fruit seed that do not ferment by after the extraction of 95% ethanol
The extract for arriving；B is the extract that fermentation promise Buddhist nun fruit seed is obtained after 95% ethanol is extracted；As can be seen from Figure 2 send out
Ferment promise Buddhist nun fruit seed is higher than promise Buddhist nun's fruit seed extraction of essential oil rate of not fermenting by the extraction of essential oil rate after the extraction of 95% ethanol.
Fig. 3：The total ion current figure of ethanol extractive fermentation promise Buddhist nun's fruit seed essential oil.Having obtained by for ethanol extractive fermentation
It is the big organic matter of retention time molecular weight more long that effect composition is less and most of.Show the Nuo Ni after processing by fermentation
Its internal chemical composition and its content have a very large change.Esters in fermentation promise Buddhist nun's fruit seed essential oil are caprylate
Class；The presence of Eugenol is found in the promise Buddhist nun's fruit seed essential oil that ferments, fermentation essential oil Eugenol content is 0.89%, is ground
Study carefully and show that Eugenol has inoxidizability.
Fig. 4：DPPH free radicals and ethanol extractive fermentation promise Buddhist nun's fruit seed essential oil lower floor active ingredient paramagnetic wave spectrum.In Fig. 4 A
DPPH can be analyzed 3 pairs of characteristic peaks at 3475-3675.Signal intensity is 140000 or so.This characteristic peak and document report
Road is consistent.Promise Buddhist nun active ingredient is without obvious characteristic peak (peak at magnetic field intensity 3600 is air peak).It is by volume fraction
Fermentation promise Buddhist nun's fruit seed essential oil fermentation lower floor DPPH solution of 4 ‰ organic solvent extraction carries out paramagnetic detection, and its result is as schemed
Shown in 4B.It is analyzed by paramagnetic wave spectrogram, it is 140000 characteristic peaks that discovery there are 3 pairs of signal intensities at 3475-3675
It has been disappeared that, it is possible thereby to be inferred to DPPH free radicals be eliminated completely.Show that the promise Buddhist nun that volume fraction is 4 ‰ is effective
Composition has high inoxidizability.
Specific embodiment
Following non-limiting examples can make one of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.Any one skilled in the art in the technical scope of present disclosure, according to
Technical scheme and its inventive concept carry out equivalent or change belongs to protection category of the present invention.
Embodiment 1
1. the fermentation process of promise Buddhist nun fruit seed is：White promise Buddhist nun's fresh fruit is plucked, cleaning puts into fermentation tank, anaerobic fermentation temperature
25 DEG C of degree control, fermentation time 150d；PH value 6.8-7.2；Using the promise Buddhist nun fruit endophyte of itself, bacterial strain CICC10883 cocoas
Beans vinegar bacillus can complete fermentation, take the solid content in tunning, standby.
2. after 60 DEG C of the promise Buddhist nun seed for fermenting is dried, 80 mesh sieves were crushed, weighed pretreated fermentation promise Buddhist nun really
Seed meal, 95% ethanol solution is prepared with absolute ethyl alcohol and ultra-pure water.It is 1 according to product and solvent ratios：10 ferment to 20g
The ethanol solutions of 200mL 95% are added in (fermentation) kernel, after immersion 6h；Supernatant liquor is tentatively obtained by suction filtration, upper strata is taken
Clear liquid obtains supernatant liquor free from foreign meter by supercentrifuge (8000r/min, time 5min).By supernatant liquor by rotation
Turn evaporimeter evaporation solvent, there is no drop to terminate evaporation when dripping in observation Rotary Evaporators condenser pipe；Carrying for solvent will be removed
Take thing high speed centrifugation again, rotating speed 8000r/min, time 5min；The oily mater that will be obtained is put into refrigerator and stands refrigeration, temperature
5 DEG C, cold preservation time is 2h, obtains fermentation promise Buddhist nun's fruit seed essential oil, and the upper and lower two-layer oily mater of the essential oil point, upper strata is shallow brown
Color, lower floor's color is dark brown, sees accompanying drawing 1, and fermentation promise Buddhist nun's extract average content is 6.1mL.
Fermentation promise Buddhist nun's fruit seed extract of organic solvent extraction, prepares and detects as stated above, obtains the total of sample
Ion flow graph.Accompanying drawing 3 and table 1 are seen to total ion current figure.Ethanol extractive fermentation promise Buddhist nun's fruit obtains active ingredient and detects 18 altogether
Material is planted, 15 kinds of materials therein are identified, the 83.33% of compound sum is accounted for, the compounds content identified accounts for extract
The 98.93% of total content.Predominantly esters and acid, Ester account for 72.09%, and esters are mainly ethyl palmitate
(40.50%), phthalic acid two (α-ethyl hexyl) ester (18.71%), acid accounts for 6.97%, and organic acid is mainly octanoic acid
(6.63%).Squalene (12.95%) in active ingredient, a kind of benzene derivate is found that in the compound of identification --- angle
It has hepatoprotective effect to MF59 (1.84%), can promote liver cell regeneration and protect liver cell, so that improve liver function, and tool
There is antitumor activity etc..Vitamin E (5.92%).Sterols material accounts for 1%, respectively campesterol (0.45%) stigmasterol
(0.55%).
Active ingredient contains the larger organic matter of molecular weight obtained by the extraction of 95% ethanol, such as in ethanol extractive fermentation and not
Fermentation promise Buddhist nun obtains linoleic acid isopropyl ester, squalene, the larger organic matter of ethyl palmitate equimolecular quantity.So according to GC-
MS data analyses can show that the primary chemical composition obtained by steam distillation is the less volatilizable organosilane ester of molecular weight
Material, the primary chemical composition obtained by the extraction of the ethanol of the method for the invention 95% is the larger organic matter of molecular weight, wherein
Squalene has the bioactivity such as antitumor.
Embodiment 2
1. the fermentation process of promise Buddhist nun fruit seed is：White promise Buddhist nun's fresh fruit is plucked, cleaning puts into fermentation tank, anaerobic fermentation temperature
25 DEG C of degree control, fermentation time 150d；PH value 6.8-7.2；Using the promise Buddhist nun fruit endophyte of itself, bacterial strain CICC10883 cocoas
Beans vinegar bacillus can complete fermentation, take the solid content in tunning, standby.
2. after 50 DEG C of the promise Buddhist nun seed for fermenting is dried, 90 mesh sieves were crushed, weighed pretreated fermentation promise Buddhist nun really
Seed meal, 95% ethanol solution is prepared with absolute ethyl alcohol and ultra-pure water.It is 1 according to product and solvent ratios：9 ferment to 20g
The ethanol solutions of 200mL 95% are added in (fermentation) kernel, after immersion 8h；Supernatant liquor is tentatively obtained by suction filtration, upper strata is taken
Clear liquid obtains supernatant liquor free from foreign meter by supercentrifuge (9000r/min, time 10min).Supernatant liquor is passed through
Rotary Evaporators evaporation solvent, does not have drop to terminate evaporation when dripping in observation Rotary Evaporators condenser pipe；Solvent will be removed
Extract high speed centrifugation, rotating speed 8500r/min, time 10min again；The oily mater that will be obtained is put into refrigerator and stands refrigeration,
4 DEG C of temperature, cold preservation time is 3h, obtains fermentation promise Buddhist nun's fruit seed essential oil, and the upper and lower two-layer oily mater of the essential oil point, upper strata is shallow
Brown, lower floor's color is dark brown, sees accompanying drawing 1, and fermentation promise Buddhist nun's extract average content is 6.7mL.
Embodiment 3
1. the fermentation process of promise Buddhist nun fruit seed is：White promise Buddhist nun's fresh fruit is plucked, cleaning puts into fermentation tank, anaerobic fermentation temperature
25 DEG C of degree control, fermentation time 150d；PH value 6.8-7.2；Using the promise Buddhist nun fruit endophyte of itself, bacterial strain CICC10883 cocoas
Beans vinegar bacillus can complete fermentation, take the solid content in tunning, standby.
2. after 55 DEG C of the promise Buddhist nun seed for fermenting is dried, 100 mesh sieves were crushed, weighed pretreated fermentation promise Buddhist nun really
Seed meal, 95% ethanol solution is prepared with absolute ethyl alcohol and ultra-pure water.It is 1 according to product and solvent ratios：11 to 2 fermentations
95% ethanol solution is added in (fermentation) kernel, after immersion 4h；Supernatant liquor is tentatively obtained by suction filtration, supernatant liquor is taken and is led to
Cross supercentrifuge (10000r/min, time 8min) and obtain supernatant liquor free from foreign meter.Supernatant liquor is steamed by rotating
Hair instrument evaporation solvent, does not have drop to terminate evaporation when dripping in observation Rotary Evaporators condenser pipe；The extract of solvent will be removed
High speed centrifugation, rotating speed 9000r/min, time 10min again；The oily mater that will be obtained is put into refrigerator and stands refrigeration, temperature 6
DEG C, cold preservation time is 4h, obtains fermentation promise Buddhist nun's fruit seed essential oil, the essential oil point upper and lower two-layer oily mater, upper strata be it is light brown,
Lower floor's color is dark brown, sees accompanying drawing 2, and fermentation promise Buddhist nun's extract average content is 5.6mL.
Active ingredient and the relative amount of each component that the ethanol extractive fermentation promise Buddhist nun fruit seed of table 1 is obtained
Embodiment 4
1. the fermentation process of promise Buddhist nun fruit seed is：White promise Buddhist nun's fresh fruit is plucked, cleaning puts into fermentation tank, anaerobic fermentation temperature
25 DEG C of degree control, fermentation time 150d；PH value 6.8-7.2；Using the promise Buddhist nun fruit endophyte of itself, bacterial strain CICC10883 cocoas
Beans vinegar bacillus can complete fermentation, take the solid content in tunning, standby.
2. after 50 DEG C of the promise Buddhist nun seed for fermenting is dried, 60 mesh sieves were crushed, weighed pretreated fermentation promise Buddhist nun really
Seed meal, 95% ethanol solution is prepared with absolute ethyl alcohol and ultra-pure water.It is 1 according to product and solvent ratios：10 to fermentation promise
95% ethanol solution is added in Buddhist nun's fruit seed, after immersion 7h；Supernatant liquor is tentatively obtained by suction filtration, supernatant liquor is taken by height
Fast centrifuge (8500r/min, time 5min) obtains supernatant liquor free from foreign meter.Supernatant liquor is steamed by Rotary Evaporators
Hair solvent, does not have drop to terminate evaporation when dripping in observation Rotary Evaporators condenser pipe；The extract for removing solvent is high again
Speed centrifugation, rotating speed 8000r/min, time 8min；The oily mater that will be obtained is put into refrigerator and stands refrigeration, 5 DEG C of temperature, during refrigeration
Between be 2h, obtain fermentation promise Buddhist nun's fruit seed essential oil, the essential oil point upper and lower two-layer oily mater, upper strata is that color is shallower, lower floor's face
Color is deeper, sees accompanying drawing 2, and fermentation promise Buddhist nun's extract average content is 6.1mL.
Taking above-mentioned fermentation promise Buddhist nun fruit seed upper strata and lower floor's essential oil carries out electron paramagnetic inoxidizability experiment, it is found that it is removed
DPPH free radicals ability is strong, shows that the fermentation promise Buddhist nun's fruit seed essential oil extracted through 95% ethanol has extremely strong antioxidation activity,
The antioxidation activity of lower floor's essential oil is more stronger than upper strata essential oil antioxidation activity simultaneously.
Claims (6)
1. it is a kind of ferment promise Buddhist nun's fruit seed essential oil extracting method, it is characterised in that：Including following operating procedure,
(1) pre-process：By the promise Buddhist nun's fruit baked seed for fermenting, crush, sieving；
(2) soak：Pretreated fermentation promise Buddhist nun's fruit seed meal is taken, the ethanol solution with 95% mixes, solid-to-liquid ratio 1:8~12,
4~10h of soak at room temperature；
(3) after suction filtration, supernatant liquor is taken after supernatant is centrifuged；
(4) rotary evaporation：Supernatant liquor is obtained into extract by Rotary Evaporators evaporation solvent；
(5) high speed centrifugation：Extract is centrifuged 3~10min with 5000~10000r/min, oily mater is obtained；
(6) refrigerate：The oily mater that will be obtained is put into 0~8 DEG C, and 1~8h of refrigeration is obtained.
2. it is according to claim 1 fermentation promise Buddhist nun's fruit seed essential oil extracting method, it is characterised in that：Described fermentation promise
The fermentation process of Buddhist nun's fruit seed is：White promise Buddhist nun's fresh fruit is plucked, cleaning puts into fermentation tank, anaerobic fermentation temperature control 20~40
DEG C, 100~150d of fermentation time；PH value 6.8-7.2；Using the promise Buddhist nun fruit endophyte of itself, bacterial strain CICC10883 cocoa beans vinegars
Bacillus completes fermentation, takes the solid content in tunning, standby.
3. it is according to claim 1 fermentation promise Buddhist nun's fruit seed essential oil extracting method, it is characterised in that：Described drying temperature
Degree is that 50~60 DEG C of sievings are 20~100 mesh sieves.
4. it is according to claim 1 fermentation promise Buddhist nun's fruit seed essential oil extracting method, it is characterised in that：Described suction filtration is
Soak is obtained into supernatant by Buchner funnel suction filtration.
5. it is according to claim 1 fermentation promise Buddhist nun's fruit seed essential oil extracting method, it is characterised in that：Step (3) is described
Centrifugation be by supernatant with 5000~10000r/min be centrifuged 3~10min.
6. it is according to claim 1 fermentation promise Buddhist nun's fruit seed essential oil extracting method, it is characterised in that：Step (4) is described
Rotary evaporation, be observe Rotary Evaporators condenser pipe in there is no drop to drip when terminate evaporation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113974039A (en) * | 2021-07-30 | 2022-01-28 | 海南师范大学 | Noni fruit enzyme without peculiar smell and preparation method thereof |
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| WO2010016777A1 (en) * | 2008-08-08 | 2010-02-11 | Robert Arthur Franich | Fractionation and modification of natural essential oils containing beta-triketones |
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| BRETT J. WEST，等: "A new vegetable oil from noni (Morinda citrifolia) seeds", 《INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY》 * |
| 曹艳花，等: "1株西沙诺尼果原浆细菌CICC 10883 的鉴定与生物学特性", 《中国食品学报》 * |
| 梁寒峭，等: "诺丽发酵果汁中抗氧化物质的研究", 《天然产物研究与开发》 * |
| 缪勇，等: "《中草药植物提取与深加工新技术实用手册 第1卷》", 31 October 2004, 天津电子出版社 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113974039A (en) * | 2021-07-30 | 2022-01-28 | 海南师范大学 | Noni fruit enzyme without peculiar smell and preparation method thereof |
| CN113974039B (en) * | 2021-07-30 | 2023-10-17 | 海南师范大学 | Noni fruit ferment free of peculiar smell and preparation method thereof |
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| Publication number | Publication date |
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| CN106701322B (en) | 2021-09-10 |
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