CN106635780A - Fluorescent quantitative PCR (polymerase chain reaction) instrument with pipetting function - Google Patents

Fluorescent quantitative PCR (polymerase chain reaction) instrument with pipetting function Download PDF

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Publication number
CN106635780A
CN106635780A CN201610894590.6A CN201610894590A CN106635780A CN 106635780 A CN106635780 A CN 106635780A CN 201610894590 A CN201610894590 A CN 201610894590A CN 106635780 A CN106635780 A CN 106635780A
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CN
China
Prior art keywords
motor
gear
pcr instrument
installation
frame
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610894590.6A
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Chinese (zh)
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CN106635780B (en
Inventor
任峻
张华忠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Yi Yi Biomedical Polytron Technologies Inc
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Shanghai Yi Yi Biomedical Polytron Technologies Inc
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Priority to CN201610894590.6A priority Critical patent/CN106635780B/en
Publication of CN106635780A publication Critical patent/CN106635780A/en
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Publication of CN106635780B publication Critical patent/CN106635780B/en
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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12Specific details about manufacturing devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/18Means for temperature control
    • B01L2300/1805Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks

Abstract

A fluorescent quantitative PCR instrument with a pipetting function belongs to the technical field of gene detection equipment. The fluorescent quantitative PCR instrument with the pipetting function comprises a flap cover, an PCR instrument host, a first gear, a first frame, a first motor, a second gear, a test tube stand, a controller, a second frame, a second motor, a fourth gear, a support, a third gear, a wheel rack, a fourth motor, a second linear motor, a second moving block, a sixth motor, a screw, a nut, a syringe, a push block, a push rod, a push board, a bearing, a belt, a roller, a first moving block, a support block, a display screen, a spring, a second electric push rod, a second shaft sleeve, a first shaft sleeve, a first electric push rod, a third motor, a support rod and a first linear motor. The PCR instrument host is provided with the flap cover; the support is arranged inside the PCR instrument host and provided with the test tube stand; the first frame is arranged on the left of the PCR instrument host and provided with the first motor, and the output shaft of the first motor is provided with the second gear. The fluorescent quantitative PCR instrument with the pipetting function has the advantage of achieving a self-pipetting function.

Description

A kind of quantitative real time PCR Instrument with pipetting function
Technical field
The present invention relates to a kind of quantitative real time PCR Instrument with pipetting function, belongs to gene detection equipment technical field.
Background technology
Gene is the One function segment on DNA molecular, is the base unit of hereditary information, is to determine all living things species The most basic factor;The birth and old age, sickness and death of gene decider are healthy, beautiful, long-lived because being operator and the regulation and control of life Person.Therefore, where where is it life, just there is gene, and the form of presence and the decline of all life is all by gene-determined, bag Include your appearance, height, body weight, the colour of skin, personality etc. inseparable with gene.Genetic test be by blood, other body fluid, Or the technology that cell is detected to DNA.
The PCR instrument of prior art does not have automatic pipetting function, uses inconvenience, in order to solve above-mentioned difficulties, Need a quantitative real time PCR Instrument with from pipetting function of exploitation.
The content of the invention
It is an object of the invention to provide a kind of quantitative real time PCR Instrument with pipetting function.
The problem to be solved in the present invention is the problem that the PCR instrument of prior art does not have automatic pipetting function.
To realize the purpose of the present invention, the technical solution used in the present invention is:
A kind of quantitative real time PCR Instrument with pipetting function, including renovating, PCR instrument main frame, first gear, the first frame, first Motor, second gear, rack for test tube, controller, the second frame, the second motor, the 4th gear, support, the 3rd gear, wheel carrier, Four motors, second straight line motor, the second movable block, the 6th motor, screw rod, nut, syringe, pushing block, push rod, push pedal, bearing, skin Band, roller, the first movable block, block, display screen, spring, the second electric pushrod, the second axle sleeve, the first axle sleeve, first electronic push away Bar, the 3rd motor, pole and first straight line motor, install on the PCR instrument main frame and renovate, mounting bracket in PCR instrument main frame, Rack for test tube is installed, the first frame is installed in PCR instrument main frame left side on frame, the first motor is installed in the first frame, the first motor it is defeated Second gear is installed, first gear is installed on second gear right side, and first gear is arranged on outside PCR instrument main frame, the first tooth on shaft First straight line motor is installed on wheel right side, and the first movable block is installed on first straight line motor, and spring, bullet are installed in the first movable block right side Display screen is installed on spring right side, and the first axle sleeve is installed on display screen, and pole is installed on the first movable block, the 3rd is installed on pole electric Machine, installs the first electric pushrod on the output shaft of the 3rd motor, the first electric pushrod is located at the first above axle sleeve, the first movable block Up and down a block is respectively installed in both sides, and the second electric pushrod is installed on a block, and two the second axle sleeves, the second axle sleeve are installed in display screen left side Positioned at the second electric pushrod right, the second frame is installed in PCR instrument main frame left side, and the second motor is installed in the second frame, and second is electric 4th gear is installed, the 3rd gear is installed on the 4th gear right side, and the 3rd gear is arranged on outside PCR instrument main frame on the output shaft of machine, Wheel carrier is installed on 3rd gear right side, and wheel carrier upper and lower ends respectively install roller, and the axle of roller is connected by shaft coupling with the 4th motor, 4th motor is arranged on wheel carrier, and belt is set on two rollers, and nut is installed on belt right side, and screw rod, spiral shell are installed on nut Bar lower end is connected by shaft coupling with the 6th motor, and the 6th motor is arranged on the second movable block, and the second movable block is arranged on the On two linear electric motors, second straight line motor is installed under the nut, and bearing is installed in screw rod upper end, and syringe, bearing are installed in nut right side Push pedal is installed on right side, installs under push pedal and pushing block is installed under push rod, push rod, and pushing block is arranged in syringe, and controller is arranged on PCR instrument Main frame left side, controller by wire respectively with the first motor, the second motor, the 3rd motor, the 4th motor, the 6th motor, the One electric pushrod and the second electric pushrod are connected.
First axle sleeve is made using elastomeric material.
Second axle sleeve is made using elastomeric material.
The wheel frame material stainless steel material is made.
It is an advantage of the invention that:Controller controls the first electric motor starting, rotates second gear, rotates first gear, Rotate display screen;Controller controls first straight line electric motor starting, moves up and down the first movable block, makes to be moved down on display screen It is dynamic;Controller controls second electric pushrod and does stretching motion, the second electric pushrod front end is inserted in the second axle sleeve, makes Display screen is inclined up or down;Controller controls the first electric pushrod and does stretching motion, inserts the first electric pushrod lower end To in the first axle sleeve;Controller controls the 3rd electric motor starting, rotates display screen;Controller controls the second electric motor starting, makes the Four gears rotate, and rotate the 3rd gear, rotate wheel carrier;Controller controls the 4th electric motor starting, rotates roller, makes belt Move up and down, move up and down syringe;Controller controls second straight line electric motor starting, moves up and down the second movable block, makes the Six motors are moved up and down;Controller controls the 6th electric motor starting, rotates screw rod, screw rod is rotating upwards, makes push pedal upper and lower Move to make syringe pipette samples.
Description of the drawings
Fig. 1 is a kind of quantitative real time PCR Instrument overall structure figure with pipetting function of the present invention;
In figure:1st, 2, PCR instrument main frame 3, first gear 4, the first frame 5, the first motor 6, second are renovated Gear 7, rack for test tube 8, controller 9, the second frame 10, the second motor 11, the 4th gear 12, support 13rd, the 3rd gear 14, wheel carrier 15, the 4th motor 16, second straight line motor 17, the second movable block 18, Six motors 19, screw rod 20, nut 21, syringe 22, pushing block 23, push rod 24, push pedal 25, bearing 26th, belt 27, roller 28, the first movable block 29, a block 30, display screen 31, spring 32, second are electric Dynamic push rod 33, the second axle sleeve 34, the first axle sleeve 35, the first electric pushrod 36, the 3rd motor 37, pole 38th, first straight line motor.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is further illustrated.
A kind of quantitative real time PCR Instrument with pipetting function of the present invention, including renovate 1, PCR instrument main frame 2, first gear 3, First frame 4, the first motor 5, second gear 6, rack for test tube 7, controller 8, the second frame 9, the second motor 10, the 4th gear 11st, support 12, the 3rd gear 13, wheel carrier 14, the 4th motor 15, second straight line motor 16, the second movable block 17, the 6th motor 18th, screw rod 19, nut 20, syringe 21, pushing block 22, push rod 23, push pedal 24, bearing 25, belt 26, roller 27, the first movable block 28th, block 29, display screen 30, spring 31, the second electric pushrod 32, the second axle sleeve 33, the first axle sleeve 34, the first electric pushrod are propped up 35th, the 3rd motor 36, pole 37 and first straight line motor 38, install on the PCR instrument main frame 2 and renovate peace in 1, PCR instrument main frame 2 Dress support 12, installs rack for test tube 7 on support 12, the left side of PCR instrument main frame 2 is installed the first frame 4, in the first frame 4 first is installed Motor 5, controller 8 controls the first motor 5 and starts, and rotates second gear 6, rotates first gear 3, revolves display screen 30 Turn;Second gear 6 is installed, first gear 3 is installed on the right side of second gear 6, and first gear 3 is installed on the output shaft of the first motor 5 Outside PCR instrument main frame 2, first straight line motor 38 is installed on the right side of first gear 3, and the first movable block is installed on first straight line motor 38 28, the control first straight line of controller 8 motor 38 starts, and moves up and down the first movable block 28, moves up and down display screen 30;The Spring 31 is installed on the right side of one movable block 28, and display screen 30 is installed on the right side of spring 31, installs the first axle sleeve 34 on display screen 30, and first Axle sleeve 34 is made using elastomeric material, and pole 37 is installed on the first movable block 28, and the 3rd motor 36, controller are installed on pole 37 8 the 3rd motors 36 of control start, and rotate display screen 30;First electric pushrod 35 is installed on the output shaft of the 3rd motor 36, the One electric pushrod 35 is located at the top of the first axle sleeve 34, and controller 8 controls the first electric pushrod 35 and does stretching motion, makes first electronic The lower end of push rod 35 is inserted in the first axle sleeve 34;First movable block respectively installs a block 29 in both sides about 28, and the is installed on a block 29 Two electric pushrods 32, controller 8 controls second electric pushrod 32 and does stretching motion, inserts the front end of the second electric pushrod 32 To in the second axle sleeve 33, display screen 30 is set to incline up or down;The left side of display screen 30 is installed by two the second axle sleeves 33, the second axle Set 33 is made using elastomeric material, and the second axle sleeve 33 is located at the right of the second electric pushrod 32, and the left side of PCR instrument main frame 2 installs second Frame 9, installs the second motor 10 in the second frame 9, controller 8 controls the second motor 10 and starts, and rotates the 4th gear 11, makes 3rd gear 13 rotates, and rotates wheel carrier 14;4th gear 11, the right side of the 4th gear 11 are installed on the output shaft of the second motor 10 3rd gear 13 is installed, the 3rd gear 13 is arranged on outside PCR instrument main frame 2, and wheel carrier 14, the material of wheel carrier 14 are installed in the right side of the 3rd gear 13 Material stainless steel material is made, and the upper and lower ends of wheel carrier 14 respectively install roller 27, and the axle of roller 27 is by shaft coupling and the 4th motor 15 It is connected, the 4th motor 15 is arranged on wheel carrier 14, and controller 8 controls the 4th motor 15 and starts, and rotates roller 27, makes belt 26 Move up and down, move up and down syringe 21;Belt 26 is set on two rollers 27, and nut 20, nut are installed in the right side of belt 26 Screw rod 19 is installed, the lower end of screw rod 19 is connected by shaft coupling with the 6th motor 18 on 20, the 6th motor 18 is installed in the second movement On block 17, controller 8 controls the 6th motor 18 and starts, and rotates screw rod 19, screw rod 19 is rotating upwards, makes push pedal about 24 Move to make the pipette samples of syringe 21, the second movable block 17 be arranged on second straight line motor 16, the control second of controller 8 is straight Line motor 16 starts, and moves up and down the second movable block 17, moves up and down the 6th motor 18;Second straight line motor 16 is arranged on Under nut 20, bearing 25 is installed in the upper end of screw rod 19, and syringe 21 is installed on the right side of nut 20, and push pedal 24, push pedal are installed in the right side of bearing 25 24 times installation push rods 23,23 times installation pushing blocks 22 of push rod, pushing block 22 is arranged in syringe 21, and controller 8 is arranged on PCR instrument main frame 2 left sides, controller 8 is electric with the first motor 5, the second motor 10, the 3rd motor 36, the 4th motor the 15, the 6th respectively by wire Machine 18, the first electric pushrod 35 are connected with the second electric pushrod 32.
Using method of the present invention:Controller 8 controls the first motor 5 and starts, and rotates second gear 6, revolves first gear 3 Turn, rotate display screen 30;The control first straight line of controller 8 motor 38 starts, and moves up and down the first movable block 28, makes display Screen 30 is moved up and down;Controller 8 controls second electric pushrod 32 and does stretching motion, inserts the front end of the second electric pushrod 32 To in the second axle sleeve 33, display screen 30 is set to incline up or down;Controller 8 controls the first electric pushrod 35 and does stretching motion, The lower end of the first electric pushrod 35 is set to be inserted in the first axle sleeve 34;Controller 8 controls the 3rd motor 36 and starts, and revolves display screen 30 Turn;Controller 8 controls the second motor 10 and starts, and rotates the 4th gear 11, rotates the 3rd gear 13, rotates wheel carrier 14; Controller 8 controls the 4th motor 15 and starts, and rotates roller 27, moves up and down belt 26, moves up and down syringe 21;Control The control second straight line of device 8 motor 16 starts, and moves up and down the second movable block 17, moves up and down the 6th motor 18;Controller 8 Control the 6th motor 18 to start, rotate screw rod 19, screw rod 19 is rotating upwards, make push pedal 24 move up and down to make syringe 21 pipette samples.

Claims (4)

1. a kind of quantitative real time PCR Instrument with pipetting function, including renovating(1), PCR instrument main frame(2), first gear(3), One frame(4), the first motor(5), second gear(6), rack for test tube(7), controller(8), the second frame(9), the second motor (10), the 4th gear(11), support(12), the 3rd gear(13), wheel carrier(14), the 4th motor(15), second straight line motor (16), the second movable block(17), the 6th motor(18), screw rod(19), nut(20), syringe(21), pushing block(22), push rod (23), push pedal(24), bearing(25), belt(26), roller(27), the first movable block(28), block(29), display screen(30)、 Spring(31), the second electric pushrod(32), the second axle sleeve(33), the first axle sleeve(34), the first electric pushrod(35), the 3rd motor (36), pole(37)With first straight line motor(38), it is characterized in that:The PCR instrument main frame(2)Upper installation is renovated(1), PCR instrument Main frame(2)Interior mounting bracket(12), support(12)Upper installation rack for test tube(7), PCR instrument main frame(2)Left side is installed by the first frame (4), the first frame(4)The first motor of upper installation(5), the first motor(5)Output shaft on second gear is installed(6), the second tooth Wheel(6)Right side is installed by first gear(3), first gear(3)Installed in PCR instrument main frame(2)Outward, first gear(3)Install on right side First straight line motor(38), first straight line motor(38)The first movable block of upper installation(28), the first movable block(28)Install on right side Spring(31), spring(31)Right side is installed by display screen(30), display screen(30)The first axle sleeve of upper installation(34), the first movable block (28)Upper installation pole(37), pole(37)The 3rd motor of upper installation(36), the 3rd motor(36)Output shaft on install first Electric pushrod(35), the first electric pushrod(35)Positioned at the first axle sleeve(34)Top, the first movable block(28)Up and down both sides are respectively pacified Dress block(29), a block(29)The second electric pushrod of upper installation(32), display screen(30)Left side is installed by two the second axle sleeves(33), Second axle sleeve(33)Positioned at the second electric pushrod(32)Right, PCR instrument main frame(2)Left side is installed by the second frame(9), the second frame (9)The second motor of upper installation(10), the second motor(10)Output shaft on install the 4th gear(11), the 4th gear(11)Right side 3rd gear is installed(13), the 3rd gear(13)Installed in PCR instrument main frame(2)Outward, the 3rd gear(13)Right side is installed by wheel carrier (14), wheel carrier(14)Upper and lower ends respectively install roller(27), roller(27)Axle by shaft coupling and the 4th motor(15)It is connected, 4th motor(15)Installed in wheel carrier(14)On, belt(26)It is set in two rollers(27)On, belt(26)Right side is installed by spiral shell It is female(20), nut(20)Upper installation screw rod(19), screw rod(19)Lower end is by shaft coupling and the 6th motor(18)It is connected, the 6th is electric Machine(18)Installed in the second movable block(17)On, the second movable block(17)Installed in second straight line motor(16)On, second straight line Motor(16)Installed in nut(20)Under, screw rod(19)Upper end is installed by bearing(25), nut(20)Right side is installed by syringe(21), axle Hold(25)Right side is installed by push pedal(24), push pedal(24)Lower installation push rod(23), push rod(23)Lower installation pushing block(22), pushing block(22) Installed in syringe(21)It is interior, controller(8)Installed in PCR instrument main frame(2)Left side, controller(8)By wire respectively with first Motor(5), the second motor(10), the 3rd motor(36), the 4th motor(15), the 6th motor(18), the first electric pushrod(35) With the second electric pushrod(32)It is connected.
2. a kind of quantitative real time PCR Instrument with pipetting function according to claim 1, is characterized in that:The first axle Set(34)Made using elastomeric material.
3. a kind of quantitative real time PCR Instrument with pipetting function according to claim 1, is characterized in that:Second axle Set(33)Made using elastomeric material.
4. a kind of quantitative real time PCR Instrument with pipetting function according to claim 1, is characterized in that:The wheel carrier (14)Material stainless steel material is made.
CN201610894590.6A 2016-10-14 2016-10-14 A kind of fluorescence quantitative PCR instrument with pipetting function Active CN106635780B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610894590.6A CN106635780B (en) 2016-10-14 2016-10-14 A kind of fluorescence quantitative PCR instrument with pipetting function

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Application Number Priority Date Filing Date Title
CN201610894590.6A CN106635780B (en) 2016-10-14 2016-10-14 A kind of fluorescence quantitative PCR instrument with pipetting function

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CN106635780A true CN106635780A (en) 2017-05-10
CN106635780B CN106635780B (en) 2019-04-02

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6006800A (en) * 1997-11-21 1999-12-28 Nichiryo Co., Ltd. Apparatus and method for automatic distribution
CN2590449Y (en) * 2002-12-23 2003-12-10 信息产业部电子第三研究所 Desk multimedia liquid crystal display screen up-down machine
CN2618260Y (en) * 2003-05-07 2004-05-26 陈周万 Displaying screen turn-over mechanism of video player
KR20070022920A (en) * 2005-08-22 2007-02-28 주식회사 대우일렉트로닉스 A controlling structure of screen angle in a display device
CN103615635A (en) * 2013-11-12 2014-03-05 湖北安心智能家居科技有限公司 Liquid crystal display screen lifting all-in-one machine
CN105931571A (en) * 2016-07-07 2016-09-07 孝感市大口贸易有限公司 Outdoor display screen mounting mechanism and mounting method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6006800A (en) * 1997-11-21 1999-12-28 Nichiryo Co., Ltd. Apparatus and method for automatic distribution
CN2590449Y (en) * 2002-12-23 2003-12-10 信息产业部电子第三研究所 Desk multimedia liquid crystal display screen up-down machine
CN2618260Y (en) * 2003-05-07 2004-05-26 陈周万 Displaying screen turn-over mechanism of video player
KR20070022920A (en) * 2005-08-22 2007-02-28 주식회사 대우일렉트로닉스 A controlling structure of screen angle in a display device
CN103615635A (en) * 2013-11-12 2014-03-05 湖北安心智能家居科技有限公司 Liquid crystal display screen lifting all-in-one machine
CN105931571A (en) * 2016-07-07 2016-09-07 孝感市大口贸易有限公司 Outdoor display screen mounting mechanism and mounting method thereof

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