CN106632667A - A household integrated collagen preparation device - Google Patents

Abstract

The invention discloses a household integrated collagen preparation device which comprises a device body, a constant-temperature reaction kettle, a separation and purification assembly, a heating kettle and a control circuit board, wherein the constant-temperature reaction kettle is arranged in the device body and used for degreasing, enzymolysis and salting-out of collagen raw materials, the separation and purification assembly is used for separating and purifying a collagen product, the heating kettle is used for heating, inactivating enzymes and sterilizing the collagen product, and the control circuit board is used for controlling the constant-temperature reaction kettle, the separation and purification assembly and the heating kettle, and the constant-temperature reaction kettle, the separation and purification assembly and the heating kettle are all connected to the control circuit board. According to the standard extraction process of the collagen of the commonly used animal collagen raw material, the equipment required in the extraction process is simplified, the integrated design is carried out, and the small integrated collagen preparation device suitable for household use is constructed, so that the structure is simple, the cost is low, and the use is more convenient.

Description

A household integrated collagen preparation device

technical field

The invention relates to the field of household equipment, in particular to a household integrated collagen preparation device.

Background technique

Collagen is the most abundant and widely distributed protein in animals, widely distributed in connective tissue, bone, visceral interstitium, muscle cavity, ligament, sclera and other parts. Collagen is rich in amino acids such as glycine, proline, and hydroxyproline that the human body needs. It widely exists in the skin, bones, muscles, cartilage, joints and hair tissues of the human body. It is the most important component of the extracellular matrix and plays a triple anti-aging role of support, repair and protection. Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields because of its good biocompatibility, biodegradability and biological activity. With the development of society and economy, people's demand for improving the quality of life is getting higher and higher, making health problems become the focus of people's attention, and collagen is therefore more and more valued by people.

Today, the market is flooded with a wide variety of collagen products of varying quality and price. Facing the many brands and various types of collagen products on the market, it is dazzling. In fact, these collagen products are all polypeptide mixtures prepared from animal collagen by enzymatic method, acid-base method or thermal degradation method. The animal collagen used in this is the most abundant and widely distributed protein in animals. The raw materials used to extract collagen in industry, such as pigskin, cowhide, fish scale, fish skin, etc., are extremely easy to obtain and the price is low, which is out of proportion to the price of collagen products on the market, which is comparable to gold.

At present, there are three main methods for extracting collagen, namely acid method, enzymatic method and alkaline method. According to different extraction methods, collagen can also be divided into acid-soluble collagen, enzyme-soluble collagen and alkali-soluble collagen. The structures, physical and chemical properties and uses of these three types of collagen are different. At present, enzymatic extraction of collagen is a direction of collagen production. Mainly because the enzymatic method has the following characteristics: (1) The conditions for extracting collagen are mild, the required equipment is simple, but the reaction rate is fast, and it can also greatly reduce environmental pollution; (2) The unique triple helical structure of collagen has the effect of protease resistance , so the use of enzymatic extraction of collagen will not destroy the structure of collagen, and the physical and chemical properties of the product are stable; (3) With the improvement of biotechnology level, protease preparations have a wide range of sources, the activity is also increasing, and the cost-effectiveness ratio is also greatly reduced.

At present, the main technological process of enzymatic extraction of collagen is as follows:

Pretreatment→enzyme extraction→salting out→separation and purification→freeze drying→finished product processing.

(1) The pretreatment process mainly refers to the process of chopping, removing impurities and degreasing collagen raw materials (pig skin, cowhide, fish scale, fish skin, etc.). This process mainly requires basic equipment such as analytical balance, tissue grinder and constant temperature water bath. (2) The enzyme extraction process refers to the process of using protease to degrade the terminal peptides in collagen fibers and the process of dissolving dispersed collagen molecules in dilute acetic acid. Since the two steps of enzymatic hydrolysis and acid dissolution are often carried out at the same time, they are collectively referred to as the enzymatic extraction process. The main equipment required for the enzyme extraction process is a constant temperature water bath. (3) The salting-out process uses the strong hydration ability of high-concentration neutral salt ions in the solution to capture the hydration layer of protein molecules, so that the protein particles lose water and coagulate to precipitate. Different proteins have different solubility in the same concentration of salt solution, so each protein component can be precipitated separately by using different concentrations of salt solution. The main equipment required for the salting out process is a constant temperature water bath. (4) The precipitate obtained after salting out is the collagen product, which may contain impurities such as material fragments, fat, inorganic ions, and miscellaneous proteins. Therefore, the collagen product obtained after salting out needs to be dissolved with dilute acetic acid solution and further purified. Collagen products can be purified by centrifugation, filtration, chromatography, adsorption and ion exchange. Centrifugation and filtration are two simple and traditional methods, and the required equipment is a compressor and a high-speed centrifuge. (5) Purified collagen can be stored in freeze-dry or dissolved in dilute acetic acid solution and stored at a low temperature of 4°C as needed. The equipment required for this process mainly includes a blast drying oven and a refrigerator.

It can be seen that the production process of extracting collagen from animal collagen raw materials is relatively complicated, and the equipment required for production includes tissue smashing and homogenizing machines, electric mixers, constant temperature water baths, compressors and high-speed centrifuges and other production equipment. It is relatively large in size, complex in structure, inconvenient to use, and expensive. It is only suitable for manufacturers with a certain production scale to realize. , It is not conducive to the operation and use of non-professionals. In addition, the existing collagen production process is controlled by the manufacturer, and the quality of the product can only be controlled by the ethical level of the enterprise and the regulatory department. However, consumers, as the actual consumers of collagen, cannot understand and master the production process of collagen, and the quality of collagen products can only be expected to have good professional ethics from production companies or due diligence from regulatory authorities.

Therefore, the prior art still needs to be improved and developed.

Contents of the invention

In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a household integrated collagen preparation device, which aims to solve the existing problems of complex structure, high cost and inconvenient use of collagen preparation devices.

Technical scheme of the present invention is as follows:

A household integrated collagen preparation device, which includes a device body, a constant temperature reaction kettle installed in the device body for collagen raw material degreasing, enzymatic hydrolysis and salting out, and a separation and purification component for the separation and purification of collagen products , a heating kettle for heating, deactivating and sterilizing collagen products, a control circuit board for controlling the constant temperature reaction kettle, separation and purification components and heating kettle, the constant temperature reaction kettle, separation and purification components and The heating kettles are all connected to the control circuit board.

The household integrated collagen preparation device, wherein, the front end of the device body is provided with an operation panel and a display panel.

The household integrated collagen preparation device, wherein, the separation and purification assembly includes a filter device for coarse filtration of the collagen product and a centrifuge for purification of the collagen product, the filter device and the centrifuge machine connection.

The household integrated collagen preparation device, wherein, the constant temperature reaction kettle includes a reaction vessel, a first temperature control circuit, and a heating plate arranged at the bottom of the reaction vessel, and the heating plate is connected to the first temperature control circuit .

The household integrated collagen preparation device, wherein the heating kettle includes a heating container, a second temperature control circuit, and an electric heating element arranged at the bottom of the heating container, and the electric heating element is connected to the second temperature control circuit .

The household integrated collagen preparation device, wherein, the filter device includes a filter container and a filter screen located in the filter container.

The household integrated collagen preparation device, wherein, the first temperature control circuit includes a temperature sensing circuit and a temperature sensor connected thereto.

In the household integrated collagen preparation device, a base is provided at the bottom of the reaction container, and the heating plate is located on the base.

The household integrated collagen preparation device, wherein, the display panel is an LCD display panel.

In the home-use integrated collagen preparation device, there are multiple filter screens, which are detachably set in the filter container.

Beneficial effects: According to the collagen standard extraction process of commonly used animal collagen raw materials, the present invention simplifies the equipment required for the extraction process, and performs an integrated design to form a small integrated collagen preparation device suitable for home use, which has a simple structure and low cost. Low, more convenient to use.

Description of drawings

Fig. 1 is a schematic structural view of a preferred embodiment of a household integrated collagen preparation device of the present invention.

Fig. 2 is a schematic structural view of the heating tank in the present invention.

Fig. 3 is a schematic structural view of the constant temperature reactor in the present invention.

Fig. 4 is a schematic structural view of the centrifuge in the present invention.

detailed description

The present invention provides a household integrated collagen preparation device. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

Please refer to Fig. 1. Fig. 1 is a schematic structural view of a preferred embodiment of a household integrated collagen preparation device of the present invention. As shown in the figure, it includes a device body 1-1, which is arranged in the device body 1-1 for Collagen raw material degreasing, enzymatic hydrolysis and salting out constant temperature reaction kettle 1-4, separation and purification components 1-6 for the separation and purification of collagen products, heating kettles for heating, deenzyme and sterilization of collagen products 1-5. A control circuit board for controlling the constant temperature reaction kettle 1-4, the separation and purification component 1-6 and the heating kettle 1-5 (viewing angle relationship is not shown), and the constant temperature reaction kettle 1-4 , separation and purification components 1-6 and heating tank 1-5 are all connected to the control circuit board.

The present invention establishes a standard extraction process for collagen of some commonly used animal collagen raw materials, and simplifies the collagen extraction process according to actual application requirements, simplifies the preparation device, and builds a small-scale integrated collagen preparation device suitable for home use, so that the collagen On-site extraction and application into thousands of households becomes possible.

For home-made collagen, in order to ensure the freshness of collagen, the prepared collagen is ready-to-use, without the need for freeze-drying and finished product processing. Therefore, the collagen extraction process can be simplified into four processes: pretreatment, enzyme extraction, salting out, and separation and purification. Therefore, the household integrated collagen preparation device of the present invention mainly realizes the following functions: (1) degreasing of collagen raw materials; (2) enzymatic hydrolysis process of collagen raw materials; (3) salting-out process of collagen raw materials; (4) Used for the separation and purification of collagen products; (5) Used to set and control the control of relevant parameters of the collagen extraction device. Therefore, the main components included in the household integrated collagen preparation device of the present invention are: separation and purification components 1-6, constant temperature reaction kettles 1-4, heating kettles 1-5 and control circuit boards. The control circuit board selects and sets specific operation steps and control methods according to the specific collagen raw material and the purpose of the collagen product.

Specifically, an operation panel 1-3 and a display panel 1-2 are provided at the front end of the device body 1-1, wherein the operation panel 1-3 can be used by the user to perform some setting operations, such as setting heating time, heating temperature , centrifugal speed, etc. Wherein the display panel 1-2 can display some information of the process currently in progress. The display panel 1-2 is an LCD display panel. The operation panel 1-3 may be a touch screen panel or a button panel.

Specifically, as shown in FIG. 3 , the internal structure of the constant temperature reaction kettle 1-4 is similar to that of the heating kettle 1-5. The constant temperature reactor 1-4 includes a reaction vessel 3-3, a first temperature control circuit, and a heating chip 3-5 arranged at the bottom of the reaction vessel 3-3, and the heating chip 3-5 is connected to the first temperature Control circuit. Specifically, the constant temperature reactor 1-4 in the present invention includes a shell 3-1, a heat conducting metal 3-2, a reaction vessel 3-3, a sealing device 3-4, a heating plate 3-5, and a first temperature control circuit. Wherein reaction vessel 3-3 is as the reaction vessel of the raw material that participates in reaction; For reducing cost, described heating plate 3-5 can adopt PTC ceramic heating plate, replaces the function of temperature sensor and electric heating element to realize the constant temperature control of reaction vessel 3-3 , so that the reaction vessel 3-3 can maintain a temperature higher than room temperature when the room temperature is too low, and can maintain a temperature lower than room temperature when the room temperature is too high. The first temperature control circuit can set the temperature of the reaction vessel 3-3 and its duration according to the collagen extraction step.

The bottom of the reaction vessel 3-3 is provided with a base, and the heating sheet 3-5 is located on the base, that is, the temperature of the reaction vessel 3-3 is controlled from the bottom. Further, the heat conduction metal 3-2 is located between the reactor body 3-1 and the reaction vessel 3-3 to realize the heat conduction function. The sealing device 3-4 is arranged on the top of the reaction vessel 3-3 to realize the sealing function.

Specifically, the separation and purification components 1-6 include a filter device for coarsely filtering the collagen product and a centrifuge for purifying the collagen product, and the filter device is connected to the centrifuge.

The separation and purification components 1-6 are used to separate and purify the collagen product obtained after salting out, so as to eliminate impurities such as material debris, fat, inorganic ions, and miscellaneous proteins. The filter device is used for coarse filtration of the collagen product; the centrifuge is used for further separation and purification of the collagen product; and the rotation speed and centrifugation time of the centrifuge can be adjusted and controlled. In addition, an ultrafiltration device can also be added to the separation and purification components 1-6 of the present invention, and the filter mesh of the ultrafiltration device has a smaller pore size to obtain collagen products with a specified molecular weight.

The structure of the centrifuge is shown in Figure 4, including a centrifugal control circuit 4-1, a housing 4-2, a geared motor 4-3, a shock absorber 4-4, a support 4-5, a rotating shaft 4-6, a centrifugal Tubes 4-7 and centrifuge racks 4-8. The centrifugal control circuit 4-1 is arranged on the inner wall of the casing 4-2, the reduction motor 4-3 is arranged above the bottom of the casing 4-2 through the bracket 4-5, and the reduction motor 4 -3 is connected to the centrifugal frame 4-8 through the rotating shaft 4-6 and drives the centrifugal frame 4-8 to rotate, and the centrifugal tube 4-7 is arranged on the centrifugal frame 4-8.

Specifically, as shown in FIG. 2 , the heating kettle 1-5 includes a heating container 2-3, a second temperature control circuit, and an electric heating element 2-7 arranged at the bottom of the heating container 2-3. Specifically, the heating kettle 1-5 specifically includes a housing 2-1, a heat conducting metal 2-2, a heating container 2-3, a sealing device 2-4, a second temperature control circuit 2-5, a temperature sensor 2- 6. Heating elements 2-7. The electric heating element 2-7 is connected to the second temperature control circuit 2-5. That is, the second temperature control circuit includes a temperature sensing circuit 2-5 and a temperature sensor 2-6 connected thereto for testing and controlling the temperature of the electric heating element 2-7.

In addition, a base is also provided at the bottom of the heating container 2-3, and the electric heating element 2-7 is located on the base, that is, the heating container 2-3 is heated from the bottom. The heat conduction metal 2-2 is located between the housing 2-1 and the heating container 2-3 to realize heat conduction function. The sealing device 2-4 is arranged on the top of the heating container 2-3 to realize the sealing function.

In the present invention, the control circuit board is used for reading and controlling the operation panel 1-3 and the display panel 1-2, and realizes the constant temperature reaction kettle 1-4, heating kettle 1-5 and Interaction and control of separation and purification components 1-6. In addition, according to the collagen extraction process of commonly used animal collagen raw materials, the control circuit board simplifies the collagen extraction process according to actual application requirements, formulates standardized extraction procedures, and can store the collagen extraction process of commonly used animal collagen raw materials. Parameters are available for users to choose, easy to operate, and intuitive, so as to realize semi-automatic operation of the collagen extraction process.

The filter device includes a filter container and a filter screen located in the filter container, wherein the filter screen can be replaced as required, for example, filter screens with different apertures can be replaced to achieve different purposes of filtration. There are multiple filter screens, and they are detachably arranged in the filter container, that is, users can choose to install different filter screens.

According to the collagen enzymatic extraction process of common animal collagen raw materials (pig skin, cowhide, chicken skin, fish scale and fish skin, etc.), the present invention can formulate standardized extraction procedures and related parameters using the device of the present invention, so as to realize the collagen extraction process. The semi-automatic operation ensures that non-professionals can operate and use the device. Standardized extraction procedures and related parameters refer to the temperature and working time of constant temperature reactor 1-4, separation and purification components 1 - Selection of the filter screen in 6, the speed and working time of the centrifuge, the temperature and working time of the heating kettle 1-5, etc.

Example 1: Using pigskin as a raw material to extract collagen peptides for consumption

The process flow is as follows:

Pretreatment→enzyme extraction→filtration→sterilization

Pretreatment: Fresh pigskin is washed and depilated, chopped, added 10% NaCl solution, put into a constant temperature reaction kettle, and soaked for 5 hours at a set temperature of 37°C. After washing with purified water three times, add Candida lipase according to the mass ratio of m(enzyme):m(pig skin)=1:100, put it into a constant temperature reactor, set the temperature at 37°C and treat for 5 hours to degrease. Pour off the treatment solution and wash 2-3 times with pure water.

Enzyme extraction: Add pepsin (or papain) (according to the mass ratio of m(enzyme):m(pigskin)=1:50) and 0.5mol/L acetic acid solution to the pretreated pigskin raw material, together Put it into a constant temperature reactor and set the temperature at 30°C for 24 hours.

Filtration: The collagen product obtained by the enzyme extraction process is filtered with a filtration device to remove impurities such as material fragments.

Sterilization: Put the filtered collagen product in a heating kettle to heat and sterilize to obtain collagen peptides. The collagen peptide can be used as a food additive or eaten directly. The molecular weight of the collagen peptide is low, which is very beneficial to the absorption of the human body and has extremely high nutritional value.

Example 2: Using pigskin as raw material to extract collagen peptides for beauty treatment

The process flow is as follows:

Pretreatment→enzyme extraction→salting out→centrifugation→separation and purification→sterilization

Pretreatment: Fresh pigskin is washed and depilated, chopped, added 10% NaCl solution, put into a constant temperature reaction kettle, and soaked for 5 hours at a set temperature of 25°C. After washing with purified water three times, add Candida lipase according to the mass ratio of m (enzyme): m (pig skin) = 1:100, put it into a constant temperature reactor, and set the temperature at 25 ° C for 10 hours to degrease. Pour off the treatment solution and wash 2~3 times with pure water.

Enzyme extraction: Add pepsin (according to the mass ratio of m(enzyme):m(pigskin)=1:50) and 0.5mol/L acetic acid solution to the pretreated pigskin raw material, and put them into a constant temperature reaction kettle together , set the temperature at 30°C for 24 hours, and filter with a filter device.

Salt-out: Add NaCl solid directly to the filtrate for salt-out (NaCl concentration is less than 5mol/L).

Centrifugal separation: Use a centrifugal device to centrifugally separate the filtrate after salting out to obtain collagen precipitates.

Separation and purification: Dissolve the collagen precipitate obtained by centrifugation in 0.5mol/L acetic acid solution, and use an ultrafiltration device to filter, separate and purify to obtain a high-purity collagen peptide solution with a certain molecular weight, and dry the high-purity collagen peptide solution , to obtain collagen peptides that can be used for beauty.

In summary, the device of the present invention adopts an integrated design, which integrates the equipment required for the four basic technological processes in the extraction process, such as pretreatment, enzyme extraction, salting out, separation and purification, into one system, which simplifies the system of complexity. The miniaturized and integrated design of the device makes it possible to realize the home application of collagen extraction in order to reduce equipment cost and operational complexity. The present invention is based on the collagen extraction process of commonly used animal collagen raw materials, and simplifies the collagen extraction process according to actual application requirements, and formulates a standardized extraction procedure to realize the semi-automatic operation of the collagen extraction process, ensuring that non-professionals can operate and Use this collagen extraction device. The device of the present invention can store the relevant parameters of the collagen extraction process of commonly used animal collagen raw materials for the user to operate and select, and is easy to operate and has good intuitiveness. The device of the invention has the advantages of simple structure, high reliability and low maintenance cost.

It should be understood that the application of the present invention is not limited to the above examples, and those skilled in the art can make improvements or transformations according to the above descriptions, and all these improvements and transformations should belong to the protection scope of the appended claims of the present invention.

Claims (10)

1. A household integrated collagen preparation device, characterized in that it comprises a device body, a constant temperature reaction kettle arranged in the device body for degreasing of collagen raw materials, enzymolysis and salting out, and used for the separation and purification of collagen products The separation and purification components of the collagen product, the heating kettle for heating, deactivating and sterilizing the collagen product, the control circuit board for controlling the constant temperature reaction kettle, the separation and purification component and the heating kettle, the constant temperature reaction kettle, Both the separation and purification components and the heating kettle are connected to the control circuit board. 2. The household integrated collagen preparation device according to claim 1, characterized in that an operation panel and a display panel are arranged at the front end of the device body. 3. The household integrated collagen preparation device according to claim 1, characterized in that, the separation and purification assembly includes a filter device for rough filtration of the collagen product and a centrifugal filter for purifying the collagen product. machine, the filter device is connected to the centrifuge. 4. The household integrated collagen preparation device according to claim 1, characterized in that, the thermostatic reactor comprises a reaction vessel, a first temperature control circuit, a heating plate arranged at the bottom of the reaction vessel, and the heating plate is connected to in the first temperature control circuit. 5. The household integrated collagen preparation device according to claim 1, wherein the heating kettle includes a heating container, a second temperature control circuit, an electric heating element arranged at the bottom of the heating container, and the electric heating element Connect the second temperature control circuit. 6. The household integrated collagen preparation device according to claim 3, characterized in that, the filter device comprises a filter container and a filter screen located in the filter container. 7. The household integrated collagen preparation device according to claim 4, wherein the first temperature control circuit comprises a temperature sensing circuit and a temperature sensor connected thereto. 8 . The household integrated collagen preparation device according to claim 4 , wherein a base is provided at the bottom of the reaction vessel, and the heating sheet is located on the base. 9. The household integrated collagen preparation device according to claim 2, wherein the display panel is an LCD display panel. 10. The household integrated collagen preparation device according to claim 6, characterized in that there are multiple filter screens and are detachably set in the filter container.