CN106614759A - Hypocrea virens wettable powder, and preparation method and application thereof - Google Patents

Hypocrea virens wettable powder, and preparation method and application thereof Download PDF

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Publication number
CN106614759A
CN106614759A CN201611207591.5A CN201611207591A CN106614759A CN 106614759 A CN106614759 A CN 106614759A CN 201611207591 A CN201611207591 A CN 201611207591A CN 106614759 A CN106614759 A CN 106614759A
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China
Prior art keywords
green meat
meat seat
seat bacterium
wettable powder
parts
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CN201611207591.5A
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CN106614759B (en
Inventor
石仕福
李肖宇
石峥
梁小文
王根豪
曾升华
严艺波
李英武
罗建辉
罗双燕
吴俊杰
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Jiangxi Tianxiang General Aviation Co Ltd
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Jiangxi Tianxiang General Aviation Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12Powders or granules
    • A01N25/14Powders or granules wettable

Abstract

The invention provides hypocrea virens wettable powder, which is prepared from the following ingredients including 2 to 10 parts of medicine active ingredients, 5.4 to 9.5 parts of auxiliary agents and 78.5 to 94.6 parts of filling materials, wherein the medicine active ingredients are hypocrea virens. The hypocrea virens wettable powder provided by the invention has the advantages of high efficiency, low toxicity, stable insecticide effect, safety on human, livestocks and crops, and high safety. Experiment results show that the insect pest prevention and control effect of the hypocrea virens wettable powder can reach 80 percent or higher to the highest degree.

Description

One kind is into green meat seat bacterium wettable powder and preparation method and application
Technical field
The present invention relates to technical field of pesticide, and in particular to one kind into green meat seat bacterium wettable powder and preparation method thereof With the application in preventing and treating crop pests.
Background technology
China is large agricultural country, and for thousands of years always based on planting industry, agricultural is the source of the basic necessities of life of the mankind, survives it This, is that other departments are able to independent and development basis, is the basis of national economy, therefore the development of agricultural will be directly affected The development of national economy.But in agricultural production, the generation of insect pest often has a strong impact on crop growth, make crops from Physiological function to institutional framework there occurs a series of change and destruction, and then cause a large amount of underproduction of crops, to grower with Huge economic loss is carried out.Therefore the effective preventing and treating to crop pests is instant, to ensure grain security and kind The economic interests of plant person.
At present the species of crop pests has many kinds, and wherein nematode can attack and parasitic plant, is attacked and parasitism Plant affects normally to grow because nematode absorbs endotrophic, and the secretion of nematode metabolism can stimulate host plant Cell and tissue, cause plant deformity.Most plants nematode encroaches on the under ground portion of plant, such as root, stem tuber, and root can Show as dross, necrosis, root is short and thick and grows thickly, cause a large amount of underproduction of crops or quality degradation.Common Plant nematode Disease has root knot nematode disease, soybean cyst nematode, wheat anguina disease, sweep stem nematode, aphelenchoides besseyi disease, oranges and tangerines half Puncture nematodiasis etc..
In prior art, usually carry out pest control disease from disease-resistant variety or chemical insecticide.Using chemical insecticide When being prevented and treated, although prevention effect is preferable, chemical insecticide has difficult degradation, easily causes soil compaction, destruction life The balance of state system, while endangering larger to people and animals.
The content of the invention
In view of this, present invention aims to insecticide does not allow problem of easy degradation, and one kind is provided into green meat Seat bacterium wettable powder and preparation method thereof, while making the wettable powder that there is safety non-toxic, preferable insecticidal effect Keep soil from packing together, be conducive to improving crop yield.
The invention provides one kind is into green meat seat bacterium wettable powder, including the component of following parts by weight:
5~8 parts of active component, the active component is into green meat seat bacterium;
5.8~8.2 parts of auxiliary agent;
89.2~73.8 parts of filler.
Preferably, including following parts by weight component:
6 parts of active component, the active component is into green meat seat bacterium;
6 parts of auxiliary agent;
88 parts of filler.
Preferably, the effective spore amount into green meat seat bacterium wettable powder is 200~40,000,000,000 CFU/ gram.
Preferably, the effective spore amount into green meat seat bacterium wettable powder is 30,000,000,000 CFU/ gram.
Preferably, spore rate >=90% living of the green meat seat bacterium into green meat seat bacterium wettable powder.
Preferably, the auxiliary agent includes the one kind or several in iso-octyl sodium succinate, Tween-60, D-425, EFW and wooden sodium Kind.
Preferably, described filler includes one or more in white carbon, calcined diatomite and calcined kaolin.
The invention provides the described preparation method into green meat seat bacterium wettable powder, comprises the following steps:
1) filler, active component and auxiliary agent are mixed, obtains compound;
2) by the step 1) compound that obtains crushed, and obtains into green meat seat bacterium wettable powder.
The present invention provide it is described into green meat seat bacterium wettable powder or described method prepare into green meat seat bacterium Application of the wettable powder in preventing and treating crop pests.
Preferably, the crop pests are tomato root-knot eelworm, tomato trialeurodes vaporariorum, aphid and underground root-knot nematode.
The invention provides one kind is into green meat seat bacterium wettable powder, including the component of following parts by weight:Active component 5 ~8 parts, the active component is into green meat seat bacterium;5.8~8.2 parts of auxiliary agent;89.2~73.8 parts of filler.In the present invention, into Green meat seat bacterium wettable powder plays the active material of insecticidal action and is into green meat seat bacterium, is reached by secreting metabolin into green meat seat bacterium To insecticidal action.And adapt in soil soon into green meat seat bacterium, steadily in the long term, therefore with efficient, low toxicity, insecticidal effect is steady It is fixed, and to people and animals and crop safety, it is safe.In addition into green meat seat bacterium as microorganism, growth metabolism process is beneficial to soil Granule is formed, and is effectively kept soil from packing together.Test result indicate that, into the effect highest of green meat seat bacterium wettable powder pest control Up to more than 80%.
Biological deposits explanation
Into green meat seat bacterium (Hypocrea virens), China Committee for Culture Collection of Microorganisms is preserved in commonly micro- Bio-Centers (CGMCC), preservation address is:China. Beijing. Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences Institute of Micro-biology, preservation date is on 08 13rd, 2013, and its deposit number is CGMCC No.7995.
Specific embodiment
The invention provides one kind is into green meat seat bacterium wettable powder, including the component of following parts by weight:
5~8 parts of active component, the active component is into green meat seat bacterium;
5.8~8.2 parts of auxiliary agent;
89.2~73.8 parts of filler.
The active component that 5~8 weight portions are preferably included into green meat seat bacterium wettable powder of present invention offer, preferably 6 Part.
In the present invention, described being preferably into green meat seat bacterium is lived with the bacterial classification that biological deposits numbering is CGMCC No.7994 Change culture to obtain.
The activation culture method into green meat seat bacterium, comprises the following steps:1) will be inoculated with into green meat seat bacterium actication of culture Cultivate in first order seed liquid culture medium, obtain primary seed solution;2) by the step 1) inoculation of the primary seed solution that obtains Fermented and cultured is carried out to secondary liquid culture medium, secondary seed solution is obtained;3) by the step 2) secondary seed solution that obtains with Solid medium is mixed, and obtains into green meat seat bacterium spore;The solid medium includes following masses percentage composition component, Wheat bran 65.00%~75.00%, dregs of beans 7.00%~12.00%, corn flour 7.60%~13.50%, husk 7.80%~ 13.40%.
The invention provides a kind of fermentation method for producing into green meat seat bacterium, will be into green meat seat bacterium actication of culture, in one-level Seed fluid nutrient mediums of saccharomycete and secondary liquid culture medium are cultivated with expanding propagation, obtain strong thalline and a certain amount of spore Son, secondary seed solution is inoculated on the solid medium that compound carbon source and nitrogen source are formed, and makes into the fast fast-growing of green meat seat bacterium Long and product spore.
The present invention will into green meat seat bacterium actication of culture, obtain activate into green meat seat bacterium bacterial classification.The present invention is to the activation Method be not particularly limited, using the technical scheme of activation well known to those skilled in the art.In the enforcement of the present invention It is described to provide for Jiangxi Tianren Ecology Co., Ltd. into green meat seat bacterium bacterial classification in example.
In the present invention, the culture medium during activation is preferably potato dextrose agar.
Obtain activating into green meat seat bacterium, the activation is inoculated into the training of first order seed liquid by the present invention into green meat seat bacterium Cultivate in foster base, obtain primary seed solution.
In the present invention, the time of the culture be preferably 35~50h, more preferably 40~45h.The temperature of described culture Degree is preferably 25~32 DEG C, more preferably 30 DEG C.Described culture is preferably carried out under illumination condition, and the intensity of illumination cultivation is 2000~3000lx.
In the present invention, the culture is carried out preferably under conditions of stirring, and the frequency of the stirring is 1~2 time/ 20min, every time the time of stirring is 1~2min, and the frequency of the stirring is 150~300r/min.
In the present invention, the incubation is ventilated, and the program of the ventilation is particularly preferred as:Into culture In 24h, throughput is preferably 10~16m3/ h, more preferably 15m3/h;Into after culture 24h, throughput is preferably 15~ 24m3/ h, more preferably 20m3/h。
In the present invention, the inoculum concentration of the culture is preferably 1~5%.
In the present invention, the initial pH value of the level liquid culture medium is preferably 5.5~6.5, more preferably 6.0.
In the present invention, the first order seed liquid culture medium preferably includes the component of following masses percentage composition:Grape Sugar 0.50%~2.00%, white granulated sugar 0.50%~2.00%, analysis for soybean powder 0.10%~0.30%, ammonium sulfate 0.10%~ 0.30%th, dipotassium hydrogen phosphate 0.05%~0.10%, potassium chloride 0.05%~0.10%, magnesium sulfate 0.01%~0.04%, sulphur Sour iron 0.00%~0.004%, balance of water;More preferably include following masses percentage composition component, glucose 1%, white granulated sugar 1%th, analysis for soybean powder 1%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, potassium chloride 0.08%, magnesium sulfate 0.02%, ferric sulfate 0.0002%, balance of water.The present invention is not particularly limited to the source of above-mentioned medicine, using well known to those skilled in the art The medicine for preparing culture medium.
After obtaining primary seed solution, the present invention is inoculated into the primary seed solution in secondary liquid culture medium is fermented Culture, obtains secondary seed solution.
The present invention is not particularly limited to the method for the inoculation, using well known inoculation described in those skilled in the art Technical scheme.
In the present invention, the inoculum concentration of the fermented and cultured is preferably 8~15%, more preferably 10%.
In the present invention, the time of described fermented and cultured be preferably 55~70h, more preferably 60~64h.
In the present invention, described fermented and cultured temperature is 28~32 DEG C, more preferably 30 DEG C.
In the present invention, described fermented and cultured need to be carried out under illumination condition, and the intensity of the illumination cultivation is 3000 ~4000lx.
In the present invention, described fermented and cultured is preferably carried out under aeration condition, and the program of the ventilation is specifically preferred For:It is inoculated within the 24h after secondary liquid culture medium, throughput is 100~180m3/ h, more preferably 120~160m3/ h, Most preferably 150m3/h;After culture 24h, throughput is 200~260m3/ h, more preferably 220~250m3/ h, most preferably 240m3/h。
In the present invention, the step 2) in culture carry out under conditions of stirring, the frequency of the stirring is 1~2 Secondary/20min, every time the time of stirring is 1~2min, and stirring frequency is 150~300r/min.
In the present invention, the pH value of zymotic fluid is not regulated and controled during described fermented and cultured, natural ph.
In the present invention, described secondary seed liquid culture medium preferably includes following masses percentage composition component:Grape Sugar 1%~3%, white granulated sugar 1%~3%, dehydrated potato powder 0.5%~1.5%, analysis for soybean powder 0.5%~1.5%, corn flour 0.3% ~1.0%, dipotassium hydrogen phosphate 0.01%~0.02%, balance of water;More preferably include following masses percentage composition component:Grape Sugar 2%, white granulated sugar 2%, dehydrated potato powder 1%, analysis for soybean powder 1%, corn flour 0.5%, dipotassium hydrogen phosphate 0.01%, balance of water.
In the present invention, the effective spore amount into green meat seat bacterium wettable powder is preferably 200~40,000,000,000 CFU/ gram, More preferably 30,000,000,000 CFU/ gram.Spore rate >=90% living into green meat seat bacterium into green meat seat bacterium wettable powder, more preferably For >=95%.
On the basis of the active component of 5~8 weight portions, what the present invention was provided includes 5.8 into green meat seat bacterium wettable powder ~8.2 parts of auxiliary agents, preferably 6 parts.The present invention is not particularly limited to the species of the auxiliary agent and source, using art technology The commercial goods of the acceptable auxiliary agent of agricultural wettable pulvis known to personnel.In the present invention, the auxiliary agent is preferably One or more in iso-octyl sodium succinate, Tween-60, D-425, EFW and wooden sodium, when the auxiliary agent is various ingredients combination When, specially two kinds, three kinds, four kinds or five kinds.In the present invention, when the auxiliary agent is that four kinds of components are combined, preferably tell Temperature -60, D-425, EFW and wooden sodium;The weight of the Tween-60, D-425, EFW and wooden sodium is than being preferably (3.0~5.0): (2.0~3.0):(0.4~1.5):(1~:4), more preferably (3.4~4.5):(1.5~2.5):(0.8~1.2):(1.5~ 3.5), most preferably 4:2:1:2.In the present invention, when the auxiliary agent is that three kinds of components are combined, preferably Tween-60, D- 425 and EFW;The Tween-60, the weight of D-425, EFW are than being preferably (3.0~5.0):(2.0~3.0):(0.4~1.5), More preferably (3.4~4.5):(1.5~2.5):(0.8~1.2), most preferably 4:2:1.
In the present invention, the wetting agent EFW is preferably alkylnaphthalene sulfonate and anion humectant mixture, the present invention The source of the wetting agent EFW is not particularly limited, using commercial goods well known to those skilled in the art.At this In bright, the auxiliary agent stablizes medicine proterties, improves drug effect.
On the basis of 5~8 parts of active components, what the present invention was provided includes 73.8~89.2 into green meat seat bacterium wettable powder The filler of part, preferably 88 parts.In the present invention, described filler is preferably in white carbon, calcined diatomite and calcined kaolin One or more.The present invention originating without special restriction to described filler, using commercial goods.In the present invention, Described filler, can arbitrary proportion mixing specifically preferably for any several in above-mentioned substance.In the present invention, fill out when described Can be white carbon and calcined diatomite when expecting to be combined for two kinds of components, be particularly preferred as 2.0~4.0 parts of white carbons and 76.5 ~90.6 parts of calcined diatomites, more preferably 2.5~3.5 parts white carbons and 80.3~88.8 parts of calcined diatomites, most preferably 3 Part white carbon and 85 parts of calcined diatomites;
Can also be white carbon and calcined kaolin when described filler is that two kinds of components are combined, it is particularly preferred as 2.0~ 4.0 parts of white carbons and 76.5~90.6 parts of calcined kaolins, more preferably 2.5~3.5 parts white carbons and 80.3~88.8 parts are forged Burn kaolin, most preferably 3 parts white carbons and 85 parts of kaolin.
Filler of the present invention has good medicine stability, can also improve the drug effect of medicine.
The invention provides into the preparation method of green meat seat bacterium wettable powder described in above-mentioned technical proposal, including following step Suddenly:
1) filler, active component and auxiliary agent are mixed, obtains compound;
2) by the step 1) compound that obtains crushed, and obtains into green meat seat bacterium wettable powder.
In the present invention, the mixing of described filler, active component and auxiliary agent is preferably filler, active component and auxiliary agent point It is not divided into 3~5 parts, more preferably 4 parts, after the 1st part of filler is mixed with the 1st part of active component, then helps with the 1st part successively Agent, the 2nd part of filler, the 2nd part of active component and the 2nd part of auxiliary agent mixing, it is by that analogy, until having added all of raw material then excellent Choosing continues to mix 20~40min, most preferably more preferably 25~35min, 30min.The present invention does not have to the equipment of the mixing There is special restriction, using mixing apparatus well known to those skilled in the art, such as mixing is filled.
In the present invention, the granularity of described filler is preferably 150~300 mesh, more preferably 200 mesh.The present invention will be described Filler preferably carries out grinding and sieving.The present invention does not have special restriction to the method for the crushing, using people in the art The technical scheme crushed known to member.In the present invention, described crushing specific is preferably air-flowing type crushing, air-flowing type The sealing pressure of crushing is preferably 0.1~0.15MPa, most preferably more preferably 0.1~0.15MPa, 0.13MPa;Air-flow The discharging sealing gland pressure that formula is crushed is preferably 0.1~0.2MPa, more preferably 0.15MPa;The pulse that air-flowing type is crushed is excellent Elect 0.4~0.6MPa, more preferably 0.5MPa as;The feed pressure of air-flowing type is preferably 0.75~0.85MPa, more preferably 0.8MPa.The present invention does not have special restriction to the equipment of the crushing, using disintegrating apparatus well known to those skilled in the art .The present invention is not particularly limited to the method sieved, using screen method well-known to those skilled in the art.
What the present invention was provided can be applicable to the aspect of crops pest control into green meat seat bacterium wettable powder, there is provided one Plant the application into green meat seat bacterium wettable powder in terms of preventing and treating crop pests.Specifically, applicable farming species Class includes cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..It is of the present invention wettable into green meat seat bacterium Property pulvis can prevent and treat tomato root-knot eelworm, tomato trialeurodes vaporariorum, aphid and underground root-knot nematode.
The present invention is not particularly limited to the described application method into green meat seat bacterium wettable powder, using this area skill Application method known to art personnel.In the present invention, the formulation rate into green meat seat bacterium wettable powder is preferably 25~ 50g/ mus, more preferably 30g/ mus.The dispenser frequency into green meat seat bacterium wettable powder is preferably 5~7 days/time.
Below in conjunction with the embodiment in the present invention, the technical scheme in the present invention is clearly and completely described.It is aobvious So, described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.Based on the reality in the present invention Example is applied, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made all belongs to In the scope of protection of the invention.
Embodiment 1
Into the preparation of green meat seat bacterium wettable powder:In terms of parts by weight, by 3 parts of white carbons, 85 parts of calcined kaolins, 5 Part is divided into respectively 4 into green meat seat bacterium, 4 parts of iso-octyl sodium succinates, 2 parts of naphthalene sulphonates and 1 part of wetting agent EFW Heap, after the 1st heap white carbon is mixed with the 1st heap calcined kaolin, then piles green meat seat bacterium and mixes with the 1st, then different pungent with the 1st heap Base sodium succinate mixes, then mixes with the 1st heap naphthalene sulphonate, then mixes with the 1st heap wetting agent EFW, then white with the 2nd heap Carbon black mixes, and by that analogy, until all raw materials have all mixed, is further continued for mixing 30min, obtains compound;Compound Jing Air-flowing type is crushed, and obtains 25 μm of powder, that is, obtain into green meat seat bacterium wettable powder.
Quality control index into green meat seat bacterium wettable powder prepared by 1 embodiment of table 2
Embodiment 2
Into the preparation of green meat seat bacterium wettable powder:In terms of parts by weight, by 3 parts of white carbons, 85 parts of calcined diatomites, 5 Part is into green meat seat bacterium, 4 parts of fatty alcohols and ethylene oxide condensate, 2 parts of sodium metnylene bis-naphthalene sulfonates, 1 part of lauryl sodium sulfate 4 heaps are divided into respectively, after the 1st heap white carbon is mixed with the 1st heap calcined diatomite, then are piled green meat seat bacterium with the 1st and are mixed, Mix with ethylene oxide condensate with the 1st heap fatty alcohol again, then mix with the 1st heap sodium metnylene bis-naphthalene sulfonate, then with the 1st heap ten Sodium dialkyl sulfate mixes, then mixes with the 2nd heap white carbon, by that analogy, until all raw materials have all mixed, is further continued for mixing 20min is closed, compound is obtained;Compound Jing air-flowing types are crushed, and obtain 20 μm of powder, that is, obtain green meat seat bacterium wettable powder Agent.
Embodiment 3
The preparation of green meat seat bacterium wettable powder:In terms of parts by weight, by 3.5 parts of white carbons, 80.3 parts of calcined kaolins, 8 parts average respectively into green meat seat bacterium, 4.5 parts of iso-octyl sodium succinates, 2.5 parts of naphthalene sulphonates and 1.2 parts of wetting agent EFW It is divided into 4 heaps, after the 1st heap white carbon is mixed with the 1st heap calcined kaolin, then piles green meat seat bacterium with the 1st and mix, then with the 1st Heap iso-octyl sodium succinate mixes, then mixes with the 1st heap naphthalene sulphonate, then mixes with the 1st heap wetting agent EFW, then with 2nd heap white carbon mixes, and by that analogy, until all raw materials have all mixed, is further continued for mixing 30min, obtains compound;It is mixed Close material Jing air-flowing types to crush, obtain 20 μm of powder, that is, obtain green meat seat bacterium wettable powder.
Embodiment 4
The test of pesticide effectiveness:This test is carried out in Shandong Province, and it is tomato to prevent and treat kind, and management is good, during root-knot nematode occurs Deng weighting.Experimental field is divided into three regions by this test, and region 1 is control group, a spray clear water;Region 2 sprays enforcement In example 2 prepare into green 2000 times of dilutions of meat seat bacterium wettable powder;Region 3 sprays 50% phoxim emulsifiable concentrate, 2000 times of dilutions Liquid, the concentration of active component is identical with region 2;Trizonal dosage is identical, and 1 day after dispenser, 3 days, each investigation in 7 days was once Borer population living, calculates Revision insect recluced rate.Concrete outcome as shown in table 2, can be drawn, the Revision insect recluced rate in region 2 by the data of table 2 Highest, shows that the insecticidal effect into green meat seat bacterium wettable powder of present invention preparation is best.
Desinsection (root-knot nematode) effect of the green meat seat bacterium wettable powder of 2 one-tenth of table
Can draw from the test data of table 2, prepared by the present invention goes down into the green meat seat bacterium wettable powder insect population of 7 days Rate may be up to 80%, and its insecticidal effect is higher than 50% phoxim emulsifiable concentrate.
As seen from the above embodiment, the present invention prepare into green meat seat bacterium wettable powder, the control rate to root-knot nematode More than 80 are may be up to, insecticidal effect is higher than effective cypermethrin.
Embodiment 5
Green meat seat bacterium wettable powder prepared by embodiment 2 is administered into tomato Tanaka, after applying 1 month, to tomato field The microbial population number of soil, the content of organic matter and crop yield are counted, and wherein Experimental Area 1 is for being administered to blank Negative control is done, pilot region 2 is the mean value of embodiment 1~3, and pilot region 3 applies 50% phoxim emulsifiable concentrate as the positive Control group.Statistics is as shown in table 3 after three Experimental Area administration fertilizer.
The green meat seat bacterium wettable powder of table 3 and control group application effect list
Tomato Tanaka's microbial population that the green meat seat bacterium wettable powders of Jing are applied can be seen that by the experimental result of table 3 Quantity is substantially better than other two control groups, and the content of organic matter also has conspicuousness to improve, while crop yield reaches 920kg/ mus, have a clear superiority compared with other two control groups.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (10)

1. one kind is into green meat seat bacterium wettable powder, including the component of following parts by weight:
5~8 parts of active component, the active component is into green meat seat bacterium;
5.8~8.2 parts of auxiliary agent;
73.8~89.2 parts of filler.
2. according to claim 1 any one into green meat seat bacterium wettable powder, it is characterised in that including following weight The component of number:
6 parts of active component, the active component is into green meat seat bacterium;
6 parts of auxiliary agent;
88 parts of filler.
3. according to claim 1 and 2 into green meat seat bacterium wettable powder, it is characterised in that it is described can into green meat seat bacterium Effective spore amount of WP is 200~40,000,000,000 CFU/ gram.
4. it is according to claim 3 into green meat seat bacterium wettable powder, it is characterised in that described into green meat seat bacterium wettable Effective spore amount of pulvis is 30,000,000,000 CFU/ gram.
5. it is according to claim 4 into green meat seat bacterium wettable powder, it is characterised in that described into green meat seat bacterium wettable Spore rate >=90% living into green meat seat bacterium of pulvis.
6. it is according to claim 1 into green meat seat bacterium wettable powder, it is characterised in that the auxiliary agent includes iso-octyl amber One or more in Meticortene Solu-Delta-cortef, Tween-60, D-425, EFW and wooden sodium.
7. according to claim 1 or 6 into green meat seat bacterium wettable powder, it is characterised in that described filler include hard charcoal One or more in black, calcined diatomite and calcined kaolin.
8. the preparation method into green meat seat bacterium wettable powder described in claim 1~8 any one, comprises the following steps:
1) filler, active component and auxiliary agent are mixed, obtains compound;
2) by the step 1) compound that obtains crushed, and obtains into green meat seat bacterium wettable powder.
9. described in claim 1~7 any one into described in green meat seat bacterium wettable powder or claim 8 method prepare Obtain into green meat seat bacterium wettable powder preventing and treating crop pests application.
10. application according to claim 1, it is characterised in that the crop pests are tomato root-knot eelworm, tomato is white Aleyrodid, aphid and underground root-knot nematode.
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