CN106538556A - A kind of biological pesticide of preventing and treating root-knot nematode - Google Patents
A kind of biological pesticide of preventing and treating root-knot nematode Download PDFInfo
- Publication number
- CN106538556A CN106538556A CN201610870021.8A CN201610870021A CN106538556A CN 106538556 A CN106538556 A CN 106538556A CN 201610870021 A CN201610870021 A CN 201610870021A CN 106538556 A CN106538556 A CN 106538556A
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- China
- Prior art keywords
- biological pesticide
- complex composition
- osthole
- day
- knot nematode
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of complex composition, the complex composition ties up rhzomorph by day and Osthole is constituted, and the weight ratio of wherein day dimension rhzomorph and Osthole is 30:1 to 1:8.The present invention also provides a kind of biological pesticide of preventing and treating root-knot nematode, and the biological pesticide includes acceptable auxiliary element on complex composition described above and agricultural chemicals.Wherein, the weight of the complex composition accounts for 0.5% the 62% of biological pesticide gross weight.Day dimension rhzomorph and Osthole mixture in the complex composition of the present invention is prevented and treated root knot nematode disease and has obvious synergistic function, improves prevention effect, also reduces the times for spraying of agricultural chemicals, reduces cost, delays the resistance to the action of a drug.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly, to a kind of biological pesticide of preventing and treating root-knot nematode, and in particular to
The complex composition and its preparation of rhzomorph and Osthole are tieed up containing day.
Background technology
As the pesticide reduction synergy action of the Ministry of Agriculture is implemented, domestic market is increasing to the demand of biological pesticide.When
Front agriculture chemical registration develops just towards efficient, low toxicity, low-residual, without security risk, without environmental risk, without application risk direction,
This causes biological pesticide to become the focus nowadays studied.The Long-Time Service of the biological pesticides such as AVM has generated strong anti-medicine
Property, have now been found that, and construct new
New Macrocyclic lactone compounds have been isolated in engineering bacteria, and the metabolin produced from the engineering bacteria --- rhzomorph is tieed up in day, and it prevents and treats
Crop pest activity is high.Osthole is a kind of vegetative insecticidal bactericide, suppresses several on insect body wall and fungal cell wall
Fourth matter deposition performance killing pests and suppressing bacteria activity, may also suppress pathogen spore and produces, sprouts, sticking, invading and germ tube elongation and work
For the nervous system of insect.Inventor has found day dimension rhzomorph and Osthole mixture preventing and treating root-knot nematode, and synergistic effect is notable.
Jing is retrieved, and does not find the relevant report of the complex composition of day dimension rhzomorph and Osthole.
The content of the invention
In view of this, in view of the shortcomings of the prior art, present invention aim at providing a kind of containing day dimension rhzomorph and snake
The complex composition of machine tool element, the complex composition and preparation have obvious synergistic function relative to single dose, and raising is killed
Worm activity, reduces the dosage of agricultural chemicals, and reduces cost delays the resistance to the action of a drug of insect.
In order to realize the present invention above-mentioned purpose, the technical scheme for adopting for：
A kind of complex composition, the complex composition ties up rhzomorph by day and Osthole is constituted；Described day dimension rhzomorph and
The weight ratio of Osthole is 30:1 to 1:8.
Preferably, the weight ratio of described day dimension rhzomorph and Osthole is 10:1 to 1:4.
The present invention also provides a kind of biological pesticide of preventing and treating root-knot nematode, and the biological pesticide includes compounding group described above
Acceptable auxiliary element on compound and agricultural chemicals；The weight of wherein described complex composition accounts for the 0.5%- of biological pesticide gross weight
62%.Preferably, the weight of complex composition described above accounts for the 3.6%-11% of biological pesticide gross weight.
On agricultural chemicals of the present invention, acceptable auxiliary element includes filler, emulsifying agent, wetting agent, dispersant etc., must
Will when can also add other conventional functionality auxiliary agents such as antifreezing agent, thickener, defoamer, disintegrant, film forming agent.
Filler is kaolin, bentonite, urea, montmorillonite.
Dispersant includes sodium metnylene bis-naphthalene sulfonate, sodium lignin sulfonate, sodium carboxymethylcellulose, methyl naphthalene sulfonic acid sodium
Formaldehyde condensation products one or more.
Wetting agent includes the one kind or several in alkylnaphthalene sulfonate, lauryl sodium sulfate, aliphatic alcohol polyethenoxy base ether
Kind.
Disintegrant is ammonium chloride, potassium sulfate, sodium sulphate.
Binding agent is ethyl cellulose, polyvinyl alcohol, starch.
The formulation of biological pesticide of the present invention is water dispersible granules, granule.
The biological pesticide of the present invention is applied in various crop root knot nematode diseases are prevented and treated, and especially prevents and treats crop such as
Root knot nematode disease on the crops such as banana, cucumber, tomato.
The complex composition and biological pesticide of the present invention is had the advantages that relative to prior art：
(1) complex composition of the invention is prevented and treated the co-toxicity coefficient of banana root-knot nematode in certain ratio range and is more than
120, show obvious synergistic function.
(2) prevention effect is improve, it is long to hold the phase, reduces agricultural chemicals times for spraying, reduce peasant's drug cost.
Specific embodiment
Below by embodiment, the invention will be further described.It should be noted that the embodiment of the present invention is only used
It is of the invention in explanation, rather than limitation of the present invention.The active compound that uses, auxiliary agent etc. are commercial.As without especially in the application
Illustrate, percentage is weight percentage.
First, the preparation of water dispersible granules
Preparation method：Day is tieed up rhzomorph, Osthole, wetting agent, dispersant, filler to be well mixed, Jing airslide disintegrating mills
After crushing, 30min is stirred, granulated in mediating and adding fluid bed, then biology of the invention is obtained with dry in drying machine, screening
The water dispersible granules of agricultural chemicals.
2nd, the preparation of granule
Day is tieed up rhzomorph, Osthole, wetting agent, dispersant, filler to be well mixed in closed compounding kettle, using bag
Embrane method is granulated, and after screened, dry, inspection, that is, obtains the granule of biological pesticide of the present invention.
BIOLOGICAL ACTIVITY EXAMPLES
Test method：
(1) preparation of root-knot nematode suspension：Picking root knot line is tied than more serious Banana Root from root-knot nematode morbidity
The pieces of an egg of worm, are positioned on the nylon nethike embrane in culture dish, plus appropriate distilled water, are placed in (25 ± 1) DEG C incubator hatching culture,
Second instar larvae is collected after 24h and is configured to nematode suspension, it is containing the second instar larvae of about 200 in every 1ml suspension, standby.
(2) day dimension rhzomorph mother liquor and Osthole mother liquor are prepared, then day dimension rhzomorph is drawn with suction pipe by prediction proportioning female
Liquid and Osthole mother liquor mixture must be standby for reagent liquid.
(3) using method of directly tagging, 24 hole biochemical test plates are taken, 1ml is respectively taken and is placed in aperture for reagent liquid, the body such as addition
Long-pending nematode suspension, blank add the clear water of equivalent.24 amount of survival and The dead quantity for checking root-knot nematode are processed,
Calculate the death rate and corrected mortality.Each process is respectively provided with 3 repetitions, averages.
The death rate=verge of death borer population/process nematode number × 100%.
Data processing calculates co-toxicity coefficient (CTC) to evaluate mixed effect using the abundant methods of Sun Yun.
Toxicity index TI (B)=(LC of standard agent A50The LC of/B agent50)×100
Actual toxicity index ATI (AB)=(LC of A50The LC of/AB50)×100
Percentage+TIs (B) of theoretical toxicity index TTI (AB)=TI (the A) × A in mixture × percentages of the B in mixture
Number
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100
Evaluation criterion：(CTC) >=120 show as synergistic effect；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120
Show as summation action.
Table 1 prevents and treats the Toxicity Determination result of banana root-knot nematode
| Composition | LC50(μg/ml) | ATI | TTI | CTC |
| Its dimension rhzomorph (A) | 1.32 | 100.00 | / | / |
| Osthole (B) | 26.80 | 4.93 | / | / |
| A:B=40:1 | 1.23 | 107.32 | 97.68 | 109.86 |
| A:B=30:1 | 1.08 | 122.22 | 96.93 | 126.09 |
| A:B=20:1 | 0.96 | 137.50 | 95.47 | 144.02 |
| A:B=10:1 | 0.75 | 176.00 | 91.36 | 192.65 |
| A:B=5:1 | 0.84 | 157.14 | 84.15 | 186.73 |
| A:B=1:1 | 1.42 | 92.96 | 52.46 | 177.19 |
| A:B=1:4 | 3.55 | 37.18 | 23.94 | 155.32 |
| A:B=1:8 | 6.26 | 21.09 | 15.49 | 136.14 |
| A:B=1:16 | 11.24 | 11.74 | 10.52 | 111.65 |
From table 1, rhzomorph and Osthole are tieed up 30 in day:1-1:Compound in the range of 8, prevent and treat banana root-knot nematode
Co-toxicity coefficient is more than 120,10:1-1:Compound in the range of 4, co-toxicity coefficient is more than 150, and synergy is more notable.
Land for growing field crops test examples
Reagent agent：
The sample of embodiment 1-7, self-control.
CK1：10% day dimension rhzomorph water dispersible granules, self-control.
CK2：10% Osthole water dispersible granules, self-control.
CK3：1% Abamectin, registration card PD20130193, be full of the limited public affairs of brightness crop science for Fushan City, Guangdong Province
Department.
CK4：1% osthole water-emulsion agent, registration card PD20141075, Anhui happiness good harvest agricultural science and technology Co., Ltd.
Cell about 60m2, per 15 plants of cell, 10 plants of random searching.
Test method：Test method and investigation method：
The root system of 60d, 120d crop rhizosphere after dispenser, investigates disease index respectively.Root system is classified, root knot is divided
For 0-4 levels, indexs at different levels are：Root system is complete, without root knot；1 grade, there is a small amount of root knot (accounting for the 11-25% of root system amount)；2 grades, root knot
Form moderate quatity (accounting for the 26%-50% of root system amount)；3 grades, root knot quantity more (accounting for the 51%-75% of root system amount)；4 grades,
Root knot special many and larger (accounting for the 76%-100% of root system amount).Root knot index and preventive effect are calculated：
Table 2 prevents and treats the field control effectiveness test result of banana root-knot nematode
3 repetition mean values of data in table.
As shown in Table 2, the biological pesticide of day dimension rhzomorph and Osthole mixture is in the case of same medicine amount, each to implement
After the medicine of example, the preventive effect of 60d, 120d is above the preventive effect of each comparison medicament, shows obvious synergistic effect.It is wherein each to implement
The preventive effect of 120d 10.7%-15.5% higher than the preventive effect of CK1 (10% day dimension rhzomorph water dispersible granules) after example medicine；Than CK2 (10%
Osthole water dispersible granules) the high 45.7%-50.5% of preventive effect；Preventive effect than CK3 (1% Abamectin) is high
21.4%-26.2%；The high 48.8%-53.4% of preventive effect than CK4 (1% osthole water-emulsion agent).After medicine, the preventive effect of 120 days is still
More than 61% can be maintained, the lasting period is long.
Claims (6)
1. a kind of complex composition, it is characterised in that the complex composition ties up rhzomorph by day and Osthole is constituted；The day
The weight ratio of dimension rhzomorph and Osthole is 30:1 to 1:8.
2. complex composition according to claim 1, it is characterised in that the weight ratio of the day dimension rhzomorph and Osthole
For 10:1 to 1:4.
3. it is a kind of preventing and treating root-knot nematode biological pesticide, it is characterised in that the biological pesticide includes the arbitrary institute of claim 1-2
Acceptable auxiliary element on the complex composition stated and agricultural chemicals；Wherein, to account for biological pesticide total for the weight of the complex composition
The 0.5%-62% of weight.
4. biological pesticide according to claim 3, it is characterised in that it is total that the weight of the complex composition accounts for biological pesticide
The 3.6%-11% of weight.
5. biological pesticide according to claim 3, it is characterised in that the formulation of the biological pesticide be water dispersible granules,
Granule.
6. application of the biological pesticide described in any one of claim 1-3 in various crop root knot nematode diseases are prevented and treated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610870021.8A CN106538556A (en) | 2016-09-30 | 2016-09-30 | A kind of biological pesticide of preventing and treating root-knot nematode |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610870021.8A CN106538556A (en) | 2016-09-30 | 2016-09-30 | A kind of biological pesticide of preventing and treating root-knot nematode |
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| Publication Number | Publication Date |
|---|---|
| CN106538556A true CN106538556A (en) | 2017-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610870021.8A Pending CN106538556A (en) | 2016-09-30 | 2016-09-30 | A kind of biological pesticide of preventing and treating root-knot nematode |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011051A (en) * | 2006-02-05 | 2007-08-08 | 武汉绿世纪生物工程有限责任公司 | Water dispersion granule for disinsection and disinfection |
| CN104910228A (en) * | 2014-03-10 | 2015-09-16 | 浙江农林大学 | 16-membered macrolide compound and application thereof |
| CN106167815A (en) * | 2016-05-30 | 2016-11-30 | 浙江农林大学 | The Preparation method and use of it dimension streptozotocin derivative |
-
2016
- 2016-09-30 CN CN201610870021.8A patent/CN106538556A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011051A (en) * | 2006-02-05 | 2007-08-08 | 武汉绿世纪生物工程有限责任公司 | Water dispersion granule for disinsection and disinfection |
| CN104910228A (en) * | 2014-03-10 | 2015-09-16 | 浙江农林大学 | 16-membered macrolide compound and application thereof |
| CN106167815A (en) * | 2016-05-30 | 2016-11-30 | 浙江农林大学 | The Preparation method and use of it dimension streptozotocin derivative |
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Application publication date: 20170329 |