CN106520606A - Compound micro-ecological preparation used for fur animals and application of preparation - Google Patents
Compound micro-ecological preparation used for fur animals and application of preparation Download PDFInfo
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Abstract
The invention discloses a compound micro-ecological preparation used for fur animals and an application of the preparation. The preparation consists of bacterial powder and a carrier, wherein the bacterial powder is prepared from lactobacillus acidophilus, saccharomycetes and bacillus subtilis according to a weight ratio of 1 to (2-3) to (3-5). The lactobacillus acidophilus, the saccharomycetes and the bacillus subtilis are matched, so that the purpose of treatment and recuperation synchronization can be achieved, and the organism immunity of the fur animals are improved while virus diseases are treated; and the effect of treating and preventing the diseases can be achieved, the symbiotic fermentation effect of lactic acid bacteria and bacillus in the compound micro-ecological preparation can be promoted, a large amount of intermediate products such as glucuronic acid, gluconic acid, acetic acid, lactic acid, amino acid and the like are generated and can replace antibiotics and chemical disease-resistant drugs in a certain range, and real green ecological breeding is realized.
Description
Technical field
The invention belongs to microbial ecological agent field, in particular to a kind of compound microecological system for fur-bearing animal
Agent, more particularly to a kind of compound micro-ecological preparation and its application for mink.
Background technology
Fur-bearing animal refers to the animal that economic benefit is produced using fur, refers mainly to mink, fox, recoon dog and rex rabbit.With
People increase year by year to fur dress ornament and the pursuit of accessories multiple demands and fashion-orientation, in recent years China's mink farming quantity
Plus, but cultural technique level is relatively low, and plant (family) adds antibiotic for a long time in feedstuff, along with not focusing on breeding environment
Control, pollute serious, it is impossible to makes fur-bearing animal resist pathogenic bacteria, virus completely and infects, makes fur-bearing animal be chronically at Asia
Health status, have had a strong impact on the pelage quality of mink and have reduced reproductive performance, considerably reduced culture benefit.
At present, some plants (family) more change products using pig, the feed addictive prescription of chicken or change fractions content
Product are packed, and commercially sell to feed mink.One side mink is variant with the physiological structure of pig, chicken, on the other hand not
Solution mink nutritional need is totally different from pig, chicken, and this feedstuff additive product can not meet mink production needs completely, for a long time
Feeding can cause mink Nutrition and Metabolism class disease to occur, and cause serious economic loss.In addition, having ermine, Vulpes, Nyctereutes procyonoidess in the market
Specialist additive product, is identical formula composition and the same feedstuff additive product of kind of the animal applications of component content, i.e., three.So
And, as mink and Vulpes, Nyctereutes procyonoidess are the animals that do not belong to together, digestive physiology structure and feeding custom are entirely different, no matter from body weight and
All there is very big difference on psychological need, so being can not targetedly to meet mink using this feedstuff additive product
Production needs.
The content of the invention
In view of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of be exclusively used in the compound micro- of fur-bearing animal
Ecological agent, especially provides a kind of compound micro-ecological preparation for being particularly well-suited to mink and its application.
In order to realize the purpose of the present invention, inventor is finally obtained by lot of experiments screening study simultaneously unremitting effort
Following technical scheme：
A kind of compound micro-ecological preparation for fur-bearing animal, said preparation are made up of mycopowder and carrier, described mycopowder by
Bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia
Burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus subtilis) CICC 10732 compare 1 according to weight:
(2-3)：(3-5) it is formulated.
Preferably, it is used for the compound micro-ecological preparation of fur-bearing animal as mentioned above, mycopowder therein is by bacillus acidophilus
(Lactobacillus acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC
AY92055 and Bacillus subtillis (Bacillus subtilis) CICC 10732 compare 1 according to weight:2.5:5 are formulated.
Preferably, be used for as mentioned above the compound micro-ecological preparation of fur-bearing animal, carrier therein selected from Rhizoma Solani tuber osi, wheat bran,
One or more of wheat flour, Carnis Bovis seu Bubali cream etc..
In addition, present invention also offers the preparation method of above-mentioned compound micro-ecological preparation, the method comprises the steps：
A, activation preserve slant strains：It is 7-7.5,100-130 DEG C of autoclaving LB of -40 minutes 20 minutes cultures with pH
Base cultivates bacillus acidophilus (Lactobacillus acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. respectively
(Pichia burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus subtilis) CICC10732,
Cultivate -20 hours 12 hours under 30-38 DEG C of condition of culture；
B, level liquid amplification culture：To bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus
Subtilis the level liquid amplification culture that) CICC 10732 is carried out respectively, inoculum concentration 10%-20%；
C, secondary liquid amplification culture：To the bacillus acidophilus (Lactobacillus after level liquid amplification culture
Acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and hay bud
Born of the same parents bacillus (Bacillus subtilis) CICC 10732 carries out secondary liquid amplification culture, inoculum concentration 10%-20% respectively；
D, preparation compound micro-ecological preparation：Streptococcus acidi lactici fermented solution, yeast fermentation liquor, fermentation of bacillus liquid are done respectively
It is dry to obtain mycopowder, it is 1 by weight:(2-3)：(3-5), after ratio is mixed and added into carrier, obtain compound micro-ecological preparation.
The present inventor screens active bacteria and its amount ratio by lot of experiments, a kind of for fur-bearing animal so as to obtain
Compound micro-ecological preparation, which can play controls the purpose for supporting synchronization, and fur-bearing animal machine is improved while viral disease is treated
Body immunity；Antibiotic and chemical disease-resistant drug can be replaced within the specific limits, real green ecological cultivation is reached.Therefore,
The present invention also provides a kind of new use of product, i.e.,：Above-mentioned compound micro-ecological preparation is in fur-bearing animal feed additive is prepared
Application.It is preferred that described fur-bearing animal is mink.
With reference to safety and the preventing and treating diseases effect of test explanation compound micro-ecological preparation of the present invention：The present invention is compound
In Qingdao Agricultural University's laboratory Jing rat acute toxicity tests, experimental group is 50 rats to microbial ecological agent, by 0.6g/10g
Body weight feeds 21 days；Blank control group is 50 rats, according to conventinal breeding.Conclusion The results show that experimental group rat is all deposited
Living, freely, hair smoothing, diet are normal for activity, and respiratory tract, eye and oral cavity etc. do not find any nosotoxicosiss without secretion beyond the region of objective existence
Shape, body weight have increase.Compare with blank control group, the rat body weight of experimental group substantially increases (P ＜ 0.05).21 day observation period
Terminate, rat dislocation is put to death and is dissected, the internal organs such as the perusal heart, liver, spleen, lung, kidney, brain, intestinal, small intestinal are showed no obvious abnormalities
Performance.In addition, rat chest gland coefficient and Spleen coefficient result show that experimental group is compared with blank control group, experimental group rat spleen
Dirty coefficient substantially increases (P ＜ 0.01), points out the compound micro-ecological preparation of the present invention to have immunological enhancement, and true border is nontoxic
Class material, degree of safety are big.
Lot of experiments shows that the compound micro-ecological preparation of the present invention has dynamic regulation animal gastrointestinal tract flora ecological
The effect of system, can cooperate with after each bacterium is compound and improve food conversion ratio, promote animal growth, strengthen fur-bearing animal immunity
Power, the ability for improving Anti-bacterium and antiviral, and then replace antibiotic diseases prevention, cure the disease, reduce residue of veterinary drug.
Specific embodiment
The present invention is described in further detail below by specific embodiment.But those skilled in the art will manage
Solution, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.In addition, unreceipted in embodiment
Particular technique operating procedure or condition person, according to the routine techniquess or condition described by document in the art or according to product
Product description is carried out.Agents useful for same or the unreceipted production firm person of instrument, be can pass through city available from conventional products.
Embodiment 1：The preparation of compound micro-ecological preparation
(1) activation preserves slant strains：It is that 7.5,120 DEG C of autoclavings LB culture medium of 30 minutes cultivates thermophilic respectively with pH
Lactobacillus lactiss (Lactobacillus acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia
Burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus subtilis) CICC 10732, cultivate at 32 DEG C
Under the conditions of cultivate 16 hours；
B, level liquid amplification culture：To bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus
Subtilis the level liquid amplification culture (50L tanks) that) CICC 10732 is carried out respectively, inoculum concentration 15%；
C, secondary liquid amplification culture：To the bacillus acidophilus (Lactobacillus after level liquid amplification culture
Acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and hay bud
Born of the same parents bacillus (Bacillus subtilis) CICC 10732, carries out secondary liquid amplification culture (500L tanks), inoculum concentration respectively
10%；
D, preparation compound micro-ecological preparation：Streptococcus acidi lactici fermented solution, yeast fermentation liquor, fermentation of bacillus liquid are done respectively
It is dry to obtain mycopowder, it is 1 by weight:2.5:5 ratio mixing.Mixing mycopowder 90kg is taken, carrier wheat bran 100kg, Carnis Bovis seu Bubali cream is added
Mix after 10kg, obtain compound micro-ecological preparation.
Embodiment 2：The preparation of compound micro-ecological preparation
(1) activation preserves slant strains：It is that 7.0,120 DEG C of autoclavings LB culture medium of 30 minutes cultivates thermophilic respectively with pH
Lactobacillus lactiss (Lactobacillus acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia
Burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus subtilis) CICC 10732, cultivate at 32 DEG C
Under the conditions of cultivate 16 hours；
B, level liquid amplification culture：To bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus
Subtilis) CICC 10732, the level liquid amplification culture for carrying out respectively (50L tanks), inoculum concentration 15%；
C, secondary liquid amplification culture：To the bacillus acidophilus (Lactobacillus after level liquid amplification culture
Acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and hay bud
Born of the same parents bacillus (Bacillus subtilis) CICC 10732, carries out secondary liquid amplification culture (500L tanks), inoculum concentration respectively
15%；
D, preparation compound micro-ecological preparation：Streptococcus acidi lactici fermented solution, yeast fermentation liquor, fermentation of bacillus liquid are done respectively
It is dry to obtain mycopowder, it is 1 by weight:2.5:5 ratio mixing.Mixing mycopowder 90kg is taken, and adds carrier wheat bran 100kg, beef
Mix after cream 10kg, after obtaining compound micro-ecological preparation.
Embodiment 3：The preparation of compound micro-ecological preparation
(1) activation preserves slant strains：It is that 7.0,120 DEG C of autoclavings LB culture medium of 30 minutes cultivates thermophilic respectively with pH
Lactobacillus lactiss (Lactobacillus acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia
Burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus subtilis) CICC 10732, cultivate at 32 DEG C
Under the conditions of cultivate 16 hours；
B, level liquid amplification culture：To bacillus acidophilus (Lactobacillus acidophilus) CGMCC
NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and Bacillus subtillis (Bacillus
Subtilis) CICC 10732, the level liquid amplification culture for carrying out respectively (50L tanks), inoculum concentration 15%；
C, secondary liquid amplification culture：To the bacillus acidophilus (Lactobacillus after level liquid amplification culture
Acidophilus) CGMCC NO.10188, Christian Breton Pichia sp. (Pichia burtonii) CCTCC AY92055 and hay bud
Born of the same parents bacillus (Bacillus subtilis) CICC 10732, carries out secondary liquid amplification culture (500L tanks), inoculum concentration respectively
15%；
D, preparation compound micro-ecological preparation：Streptococcus acidi lactici fermented solution, yeast fermentation liquor, fermentation of bacillus liquid are done respectively
It is dry to obtain mycopowder, it is 1 by weight:2.5:5 ratio mixing.Mixing mycopowder 90kg is taken, and adds carrier wheat bran 100kg, beef
Mix after cream 10kg, obtain compound micro-ecological preparation.
In order to shadow of the mink compound micro-ecological preparation to mink body weight, immunity and intestinal health is further described
Ring, packet control is carried out to mink below.
Test mink, is that Dalian is black, is provided by Qingdao agricultural science and technology company limited Fur Animal Feeding base.Choose
150 ages in days son ermine 60, is randomly divided into 6 groups by sex and body weight, per group of 5 repetitions, often repeatedly 2, male and female half and half, test from
From 20 days April in 2016,24 end of day of May, 35 days altogether.Per group little mink point cage nursing, free choice feeding and drinking-water.
Colony counting adopts colony counting method.25mL bacterium solutions are taken with the graduated cylinder through high pressure in super-clean bench it is placed in and fills
In the wide mouthed bottle of 250mL normal saline, it is 1 fully to shake up proportional:10 diluent.It is 1.0mL through high pressure with volume
Suction pipe draw 1.0mL be placed in the normal saline for filling 9mL, carry out ten times dilution, repeat aforesaid operations until bacterium solution dilute
Multiple is 109.With suction pipe draw 1.0mL bacterium solution be placed in through high pressure, baking after plate in, while will be cooled to 46 DEG C it is left
Right Nutrient agar is poured in plate, while mobile plate is sufficiently mixed solution.Flat board is placed in calorstat and cultivates 24h meters
Number, repetition do three times.
Inoculation：Experimental animal is randomly divided into into six groups of A, B, C, D, E, F, per group of 5 repetitions.Mink is exempted from
Epidemic disease, the antigen and Freund's complete adjuvant 0.1mL of every mink injection equal-volume mixing for the first time.Every mink note after one week
Penetrate the antigen and incomplete Freund's adjuvant adjuvant 0.1mL of equal-volume mixing, interval every mink duplicate injection equal-volume after a week
The antigen of mixing and incomplete Freund's adjuvant adjuvant 0.1mL.
(1) to mink gavage process packet and dosage
Table 1：Different treatments to testing mink
(2) impact of the different tests group to mink body weight
Table 2：Body weight increase situation (the unit of experimental period mink：g)
Remarks：Difference is represented not significantly (P ＞ 0.05) with occurring the identical upper target of lower case in a line, it is little with difference
Female expression diversity write significantly (P ＜ 0.05).
Interpretation of result：Before testing as can be seen from Table 2, the body weight of mink is basically identical.During test, matched group and examination
The mink body weight for testing group increases substantially, and the body weight of test group mink is generally than fast, significant difference (the P ＜ of matched group growth
0.05).The body weight increase situation of mink before and after test is analyzed, averagely increasing day by day after drawing test group mink before the test
It is fast that anharmonic ratio matched group increases, especially public ermine, and average daily gain has reached 22.44g.
(3) impact of the different tests group to mink organ index
Table 3：The internal organs index of the little mink of different disposal group
Data analysiss：Statistical analysiss are carried out to the internal organs of mink with spss softwares, using the method for multiple comparisons.
Drawn by table 3, each internal organs index of mink of composite bacteria agent capable F groups compared with control group A group extremely significantly (P ＜ 0.01) and
It is significantly higher than other single bacterium groups (P ＜ 0.05).
(4) ELISA test result analysis
Table 4：The serum bursal disease virus antibody OD values of different disposal group mink
By shown in table 4 and Fig. 1, it can be deduced that the OD values for measuring for the first time other test groups in addition to E groups compare
It is close to, i.e. the antibody content obvious difference (P ＞ 0.05) after first time is immune in mink body；Second and third time it is immune after be combined
Apparently higher than other test groups, microbial inoculum group OD numerical value illustrates that its internal antibody content numerical value compared with other test groups is elevated more
Greatly (P ＜ 0.05).
(5) mucosal thickness of the different tests group to mink jejunum, the impact of villus length
Draw in light Microscopic observation, bacillus cereuss group, composite bacteria agent capable liquid group and matched group mink jejunal mucous membrane structure compare
More complete and well arranged, intestinal villus are completely neat.It can be seen that have obvious branch, the striated border of mucous membrane of small intestine epithelial surface
Structure straight uniform.Compared with matched group, the little mink jejunal mucous membrane fine hair arrangement of composite bacteria agent capable group is even closer, and fine hair compares
It is long.
This result of the test shows no matter feed water ermine gavage bacillus cereuss group, lactic acid bacteria group or gavage composite bacteria agent capable group
The growth of mink can be promoted, but the mink body weight of gavage composite bacteria agent capable group is much larger than compared with other test groups after two weeks
Other test groups.
(6) impact of the different tests group to mink internal organs index
Composite bacteria agent capable group difference compared with matched group in terms of the growth of little mink internal organs is promoted can be drawn by test
Heteropole is significantly (P ＜ 0.01).Gavage lactic acid bacteria group, bacillus cereuss group and the impact to the organ index difference compared with matched group shows
Write (P ＜ 0.05).Show that composite bacteria agent capable group is more notable to effect in terms of the internal organs index of mink.
(7) impact of the different tests group to bursal antibody potency
The mink antibody titer of this result of the test display different tests group significant difference compared with matched group, but compound bacteria
The serum titer of agent group mink is increasing for the first time and respectively 29% and 30% for the second time compared with matched group, with other test group phases
It is more larger than change in value.Show that compound probiotic effect in terms of the potency of antibody is strengthened is more notable.
In sum, growth-weight gain of the present invention to mink, the growth of internal organs, the rising of chicken bursal antibody potency
And the Microvillares length of intestinal and intestinal wall thickness have obvious facilitation, and the performance of mink is greatly improved.
Claims (5)
1. a kind of compound micro-ecological preparation for fur-bearing animal, it is characterised in that said preparation is made up of mycopowder and carrier, described
Mycopowder by bacillus acidophilus CGMCC NO.10188, Christian Breton Pichia sp. CCTCC AY92055 and Bacillus subtillis CICC
10732 compare 1 according to weight:(2-3)：(3-5) it is formulated.
2. the compound micro-ecological preparation of fur-bearing animal is used for according to claim 1, it is characterised in that described mycopowder is by thermophilic
Lactobacillus lactiss CGMCC NO.10188, Christian Breton Pichia sp. CCTCC AY92055 and Bacillus subtillis CICC 10732 according to
Weight compares 1:2.5:5 are formulated.
3. the compound micro-ecological preparation of fur-bearing animal is used for according to claim 1, it is characterised in that described carrier is selected from
One or more of Rhizoma Solani tuber osi, wheat bran, wheat flour and Carnis Bovis seu Bubali cream.
4. application of the compound micro-ecological preparation described in any one of claim 1-3 in fur-bearing animal feed additive is prepared.
5. application according to claim 4, it is characterised in that described fur-bearing animal is mink.
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| CN112006170A (en) * | 2020-07-31 | 2020-12-01 | 黑龙江省农业科学院畜牧研究所 | Feed additive for promoting production performance of fur-bearing animals and application thereof |
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