CN106497782A - A kind of air microorganism sampling device - Google Patents

A kind of air microorganism sampling device Download PDF

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Publication number
CN106497782A
CN106497782A CN201610872584.0A CN201610872584A CN106497782A CN 106497782 A CN106497782 A CN 106497782A CN 201610872584 A CN201610872584 A CN 201610872584A CN 106497782 A CN106497782 A CN 106497782A
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air
module
sampling
silica gel
continuation
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CN106497782B (en
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马迎飞
麦国琴
樊继强
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Shenzhen Biological Manufacturing Industry Innovation Center Co ltd
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Shenzhen Institute of Advanced Technology of CAS
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0004Gaseous mixtures, e.g. polluted air

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Electromagnetism (AREA)
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  • Combustion & Propulsion (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present embodiments relate to technical field of bioengineering, discloses a kind of air microorganism sampling device, including:Continuation of the journey air-breathing module, sampling area extension module and monitoring module;There is continuation of the journey air-breathing module inspiratory component, inspiratory component there is air entry end;There is sampling area extension module silica gel funnel, silica gel funnel there is first port and second port;The air entry end of inspiratory component is closely coupled with the first port of silica gel funnel;The surface of inspiratory component is interlockingly connected with monitoring module;The second port of silica gel funnel is used for fixed sample module;When inspiratory component works, air flows out air microorganism sampling device after sequentially passing through sampling module, sampling area extension module and continuation of the journey air-breathing module from outside sampling module;Monitoring module obtains environmental quality data for the air that monitoring flows through continuation of the journey air-breathing module.Implement the embodiment of the present invention, can solve the problem that the problem that capture rate is low in sampling process, sampling efficiency is low.

Description

A kind of air microorganism sampling device
Technical field
A kind of the present invention relates to technical field of bioengineering, more particularly to air microorganism sampling device.
Background technology
While development with industrial technology, the atmosphere pollution caused by waste gas produced in industrial production is also more and more tighter Weight, wherein, haze is the general statement to various suspended particulate contaminants content overproofs in air, and its suspended particulate is both pollution Thing, is the carrier of heavy metal, polycyclic aromatic hydrocarbon, microorganism etc. again.In recent years, the severe air pollution that China is caused because of haze weather Phenomenon is related to that region is wide, intensity is high, the duration is long, especially in the respiratory disease outpatient service disease that haze sky is related to haze Example is dramatically increased, and clinically, the generation of respiratory disease is usually caused by pathogenic microorganism.
Thus, gathering and analyze the content of microorganism, species etc. under haze weather becomes extremely important, and common air is micro- Biological sampling device have natural sedimentation, filter detention formula sampler, solid impact formula sampler, liquid knockout formula sampler and Electrostatic calmness class sampler etc..However, these sampling apparatuses are it is possible that capture rate is low, sampling efficiency is low problem.
Content of the invention
The embodiment of the invention discloses a kind of air microorganism sampling device, can solve the problem that in sampling process capture rate low, The low problem of sampling efficiency.
The embodiment of the invention discloses a kind of air microorganism sampling device, including:
Continuation of the journey air-breathing module, sampling area extension module and monitoring module:
The continuation of the journey air-breathing module has inspiratory component, and the inspiratory component has air entry end;
There is the sampling area extension module silica gel funnel, the silica gel funnel there is first port and second port;
The air entry end of the inspiratory component is closely coupled with the first port of the silica gel funnel;The inspiratory component Surface is interlockingly connected with the monitoring module;The second port of the silica gel funnel is used for fixed sample module;
When the inspiratory component works, air sequentially passes through the sampling module, the sampling from outside the sampling module The air microorganism sampling device is flowed out after area extension module and the continuation of the journey air-breathing module;The monitoring module is used for Monitoring flows through the air of the continuation of the journey air-breathing module and obtains environmental quality data.
Used as a kind of optional embodiment, described device also includes the sampling module, for flowing through in the air During the sampling apparatus so that the microorganism in the air is enriched with the sampling module.
Used as a kind of optional embodiment, also there is the continuation of the journey air-breathing module suction modulation part, the air-breathing to adjust Section part connects the inspiratory component, is adjusted for the air-breathing wind speed to the inspiratory component.
As a kind of optional embodiment, the first port of the air entry end of the inspiratory component and the silica gel funnel Closely coupled, including:
The air entry end of the inspiratory component is closely coupled by wide adhesive tape with the first port of the silica gel funnel.
Used as a kind of optional embodiment, the sampling module has collecting part and fixed component;
The collecting part is sterilized double-deck electrostatic precipitation paper, removes for flowing through the double-deck electrostatic in the air The microorganism being enriched with during dirt paper in the air;
The fixed component is used for fixing the double-deck electrostatic precipitation paper and the silica gel funnel.
Used as a kind of optional embodiment, the fixed component is sterilized rubber band.
Used as a kind of optional embodiment, the double-deck electrostatic precipitation paper has ground floor electrostatic precipitation paper and the second layer Electrostatic precipitation paper, the ground floor electrostatic precipitation paper and the second layer electrostatic precipitation paper overlapped;
The sampling module is fixed in the second port of the silica gel funnel, including:
The ground floor electrostatic precipitation paper or the second layer electrostatic precipitation paper are fixed by the sterilized rubber band In the second port of the silica gel funnel.
Used as a kind of optional embodiment, the surface of the inspiratory component is provided with a handle.
Used as a kind of optional embodiment, the monitoring module flows through the air of the continuation of the journey air-breathing module for monitoring Environmental quality data is obtained, including:
The monitoring module obtains PM2.5 concentration and air matter for the air that monitoring flows through the continuation of the journey air-breathing module Volume index.
Used as a kind of optional embodiment, the continuation of the journey air-breathing module also has lithium battery and charger.
Compared with prior art, the embodiment of the present invention has the advantages that:
In air microorganism sampling device provided in an embodiment of the present invention, air flow speed can be improved by inspiratory component Rate, so that improve sampling efficiency;Sampling area can be expanded using silica gel funnel, capture rate is improved;The air-breathing of inspiratory component Mouth end is closely coupled with the first port of silica gel funnel, effectively prevent the generation of gas leak phenomenon in sampling process;In addition, monitoring Module can also obtain the quality of air environment data for flowing through air microorganism sampling device.It can be seen that, implement the embodiment of the present invention, Can effectively solve the problem that the problem that capture rate is low in sampling process, sampling efficiency is low.
Description of the drawings
For the technical scheme being illustrated more clearly that in the embodiment of the present invention, below by to be used needed for embodiment Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present invention, for ability For the those of ordinary skill of domain, on the premise of not paying creative work, can be other attached to be obtained according to these accompanying drawings Figure.
Fig. 1 is a kind of structural representation of air microorganism sampling device disclosed in the embodiment of the present invention;
Fig. 2 is the structural representation of the continuation of the journey air-breathing module shown in Fig. 1;
Fig. 3 is the structural representation of the silica gel funnel shown in Fig. 1;
Fig. 4 is the structural representation of the monitoring module shown in Fig. 1;
Fig. 5 is the structural representation of the sampling module shown in Fig. 1.
Specific embodiment
Accompanying drawing in below in conjunction with the embodiment of the present invention, to the embodiment of the present invention in technical scheme carry out clear, complete Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on this Embodiment in invention, the every other reality obtained under the premise of creative work is not made by those of ordinary skill in the art Example is applied, the scope of protection of the invention is belonged to.
The embodiment of the invention discloses a kind of air microorganism sampling device, can solve the problem that in sampling process capture rate low, The low problem of sampling efficiency.
Fig. 1~Fig. 5 is referred to, Fig. 1 is that a kind of structure of air microorganism sampling device is shown disclosed in the embodiment of the present invention It is intended to, Fig. 2 is the structural representation of the continuation of the journey air-breathing module shown in Fig. 1, and Fig. 3 is the structure of the silica gel funnel shown in Fig. 1 Schematic diagram, Fig. 4 are the structural representations of the monitoring module shown in Fig. 1, and Fig. 5 is that the structure of the sampling module shown in Fig. 1 is shown It is intended to.
As shown in Fig. 1~Fig. 5, the air microorganism sampling device can include:
Continuation of the journey air-breathing module 1, sampling area extension module 2 and monitoring module 3;
As shown in Fig. 2 there is continuation of the journey air-breathing module 1 inspiratory component 011, inspiratory component 011 there is air entry end 012;
There is sampling area extension module 2 silica gel funnel 021, silica gel funnel 021 as shown in Figure 3 there is first port 022 With second port 023;
The air entry end 012 of inspiratory component 011 is closely coupled with the first port 022 of silica gel funnel 021;Inspiratory component 011 surface is interlockingly connected with monitoring module 3;The second port 023 of silica gel funnel 021 is used for fixed sample module;
When inspiratory component 011 works, air sequentially passes through sampling module 4, sampling area extension module from outside sampling module 4 2 and continuation of the journey air-breathing module 1 after flow out air microorganism sampling device;Monitoring module 3 flows through continuation of the journey air-breathing module 1 for monitoring Air obtain environmental quality data.
In the embodiment of the present invention, above-mentioned air microorganism sampling device can also include sampling module 4, in air stream During sampled device 4 so that the microorganism in air is enriched with sampling module 4.
It should be noted that the air microorganism sampling device can also be by user according to need including sampling module 4 Sampling module to be provided for oneself 4, the embodiment of the present invention is not construed as limiting.
In the embodiment of the present invention, also there is continuation of the journey air-breathing module 1 suction modulation part 013, suction modulation part 013 to connect Inspiratory component 011, is adjusted for the air-breathing wind speed to inspiratory component 011.
For example, when suction modulation part 013 is adjusted to the air-breathing wind speed of inspiratory component 011, user can be led to The button that crosses on suction modulation part 013 selects different wind speed, such as, user can select at a high speed, or middling speed, and or Person's low speed.In the case that microbes in air concentration is low, can be obtained as needed more by selecting different wind speed, user Big air flow rate, so that effectively increase air microbe capture rate.
In the embodiment of the present invention, the air entry end 012 of inspiratory component 011 is tight with the first port 022 of silica gel funnel 021 It is connected, including:
The air entry end 012 of inspiratory component 011 is closely coupled by wide adhesive tape with the first port 022 of silica gel funnel 021.
Wherein, sampling area can be expanded using silica gel funnel 021, so as to effectively increase sampling efficiency, and is passed through The air entry end of inspiratory component is closely connected by wide adhesive tape with the first port of silica gel funnel, so as to flow through inspiratory component when air When 011, the generation of gas leak phenomenon is effectively prevent.
In the embodiment of the present invention, sampling module 4 has collecting part 041 and fixed component 042;
Collecting part 041 is sterilized double-deck electrostatic precipitation paper, for flowing through above-mentioned double-deck electrostatic precipitation paper in air When enriched air in microorganism;
Fixed component 042 is used for fixed bilayer electrostatic precipitation paper and silica gel funnel 021.
In the embodiment of the present invention, fixed component 042 is sterilized rubber band.
Wherein, as electrostatic precipitation sheet hole footpath is small and dense and ventilative, homogeneous, with good microorganism adsorption energy Power, and electrostatic precipitation paper and rubber band are carried out sterilization treatment, the efficiency of sampled air microorganism can be improved.
In the embodiment of the present invention, double-deck electrostatic precipitation paper has ground floor electrostatic precipitation paper and second layer electrostatic precipitation paper, Ground floor electrostatic precipitation paper and second layer electrostatic precipitation paper are overlapped, not shown in figure;
Sampling module 4 is fixed in the second port 023 of silica gel funnel 021, including:
Ground floor electrostatic precipitation paper or second layer electrostatic precipitation paper are fixed on silica gel funnel 021 using sterilized rubber band Second port 023 on.
Wherein, using double-deck electrostatic precipitation paper, one layer is used for fixing with silica gel funnel, and another layer is used for the micro- life of enriched air Thing.In addition, when environment of sampling changes, user can also be sampled by changing double-deck electrostatic precipitation paper, not only side Just quick, but also can reduce due to changing the pollution that electrostatic precipitation paper tape comes not in time.
In the embodiment of the present invention, the surface of inspiratory component 011 is provided with a handle 014.
Wherein, by arranging handle 014, the flexibility of the air microbe device can be improved, is easy to user to take with oneself Band.
In the embodiment of the present invention, monitoring module 3 obtains environmental quality number for the air that monitoring flows through continuation of the journey air-breathing module 1 According to, including:
Monitoring module 3 obtains PM2.5 concentration for the air that monitoring flows through continuation of the journey air-breathing module 1 and air quality refers to Number.
Wherein, PM2.5 concentration and air quality index are shown by monitoring module 3, can is user study air matter Amount provides information with the relation of air microbe.
In the embodiment of the present invention, continuation of the journey air-breathing module 1 also has lithium battery and a charger, not shown in figure.
Wherein, by arranging lithium battery, can continue a journey for above-mentioned air microorganism sampling device, can by arranging charger Think that above-mentioned air microorganism sampling device charges, so as to be beneficial to user's carrying, improve above-mentioned air microorganism sampling device Flexibility.
In order to the practicality of the air microorganism sampling device is more clearly from described, said in actual scene below Bright.
Adopted in Shenzhen's Buji near streets for 19 points in August in 2016 24 days with the air microorganism sampling device Sample.
1st, electrostatic precipitation paper and rubber band are carried out sterilization treatment, has weighed one (any one layer in i.e. above-mentioned bilayer) The weight of the electrostatic precipitation paper of sterilizing;
Wherein, the electrostatic precipitation paper that has weighed is above-mentioned ground floor electrostatic precipitation paper, and unweighed electrostatic precipitation paper goes up State second layer electrostatic precipitation paper.
2nd, above-mentioned sterilized double-deck electrostatic precipitation paper is fixed on silica gel funnel device using sterilized rubber band;
Wherein, ground floor electrostatic precipitation paper is overlapped with second layer electrostatic precipitation paper, and second layer electrostatic precipitation paper is solid The second port of fixed and silica gel funnel.
3rd, start inspiratory component, and be adjusted to top gear, the collecting part in air microorganism sampling device starts Enriched air microorganism, the monitoring module of air microorganism sampling device show that PM2.5 concentration is:65ug/m3
4th, after air microorganism sampling device operation 0.5h, the electrostatic precipitation paper that has weighed is taken off, is weighed, so Afterwards weight twice is subtracted each other, obtains the sampling weight of air microbe:120mg.
It can be seen that, implement the embodiment of the present invention, user when air microbe sampling is carried out, using the air microbe Sampling apparatus, not only can expand sampling area, improve capture rate and sampling efficiency, and when sampling environment changes When, it is only necessary to sampling is re-started by changing electrostatic precipitation paper.In addition, the air microorganism sampling device can be continued a journey making With, facilitate user carry, and by monitoring module record environmental quality data, can also further study air quality for user Information is provided with the relation of microorganism.
Above a kind of air microorganism sampling device disclosed in the embodiment of the present invention is described in detail, herein should Principle of the invention and embodiment are set forth with specific case, the explanation of above example is only intended to help and manages The solution method of the present invention and its core concept;Simultaneously for one of ordinary skill in the art, according to the thought of the present invention, Will change in specific embodiment and range of application, in sum, this specification content is should not be construed as to this Bright restriction.

Claims (10)

1. a kind of air microorganism sampling device, it is characterised in that include:
Continuation of the journey air-breathing module, sampling area extension module and monitoring module:
The continuation of the journey air-breathing module has inspiratory component, and the inspiratory component has air entry end;
There is the sampling area extension module silica gel funnel, the silica gel funnel there is first port and second port;
The air entry end of the inspiratory component is closely coupled with the first port of the silica gel funnel;The surface of the inspiratory component It is interlockingly connected with the monitoring module;The second port of the silica gel funnel is used for fixed sample module;
When the inspiratory component works, air sequentially passes through the sampling module, the sampling area from outside the sampling module The air microorganism sampling device is flowed out after extension module and the continuation of the journey air-breathing module;The monitoring module is used for monitoring The air for flowing through the continuation of the journey air-breathing module obtains environmental quality data.
2. device according to claim 1, it is characterised in that described device also includes the sampling module, in institute When stating air and flowing through the sampling apparatus so that the microorganism in the air is enriched with the sampling module.
3. device according to claim 1, it is characterised in that the continuation of the journey air-breathing module also has suction modulation part, The suction modulation part connects the inspiratory component, is adjusted for the air-breathing wind speed to the inspiratory component.
4. device according to claim 1, it is characterised in that the air entry end of the inspiratory component and the silica gel funnel First port closely coupled, including:
The air entry end of the inspiratory component is closely coupled by wide adhesive tape with the first port of the silica gel funnel.
5. device according to claim 2, it is characterised in that the sampling module has collecting part and fixed component;
The collecting part is sterilized double-deck electrostatic precipitation paper, for flowing through the double-deck electrostatic precipitation paper in the air When be enriched with microorganism in the air;
The fixed component is used for fixing the double-deck electrostatic precipitation paper and the silica gel funnel.
6. device according to claim 5, it is characterised in that the fixed component is sterilized rubber band.
7. device according to claim 6, it is characterised in that the double-deck electrostatic precipitation paper has ground floor electrostatic precipitation Paper and second layer electrostatic precipitation paper, the ground floor electrostatic precipitation paper and the second layer electrostatic precipitation paper overlapped;
The sampling module is fixed in the second port of the silica gel funnel, including:
The ground floor electrostatic precipitation paper or the second layer electrostatic precipitation paper are fixed on by institute by the sterilized rubber band State in the second port of silica gel funnel.
8. device according to claim 1, it is characterised in that the surface of the inspiratory component is provided with a handle.
9. device according to claim 1, it is characterised in that the monitoring module flows through the continuation of the journey air-breathing for monitoring The air of module obtains environmental quality data, including:
The monitoring module obtains PM2.5 concentration for the air that monitoring flows through the continuation of the journey air-breathing module and air quality refers to Number.
10. the device according to any one of claim 1~9, it is characterised in that the continuation of the journey air-breathing module also has lithium electricity Pond and charger.
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Patentee before: Shenzhen advanced science and technology Cci Capital Ltd.

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