CN106492896A - A kind of liquid-transfering device for volatile liquid - Google Patents

A kind of liquid-transfering device for volatile liquid Download PDF

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CN106492896A
CN106492896A CN201611223023.4A CN201611223023A CN106492896A CN 106492896 A CN106492896 A CN 106492896A CN 201611223023 A CN201611223023 A CN 201611223023A CN 106492896 A CN106492896 A CN 106492896A
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pipetting
tube
liquid
piston
connecting plate
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CN106492896B (en
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邢世平
胡友彪
吴亚萍
王月越
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Anhui University of Science and Technology
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Anhui University of Science and Technology
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56Labware specially adapted for transferring fluids
    • B01L3/567Valves, taps or stop-cocks
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/141Preventing contamination, tampering
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/142Preventing evaporation

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

本发明公开了一种针对挥发性液体的移液装置。在实验操作过程中,移液是常见的操作之一。针对现有的移液装置不能避免挥发性液体直接与外部空气接触,设计了一种针对挥发性液体的移液装置,其中移液塞1卡在进液管上,进液管与导液管通过连接板1相连,进液管与导气管在同一水平上,挡板位于导液管与导气管分界处,橡皮管连接导气管与移液桶,活塞杆与活塞固结在一起,出液管通过连接板2与导液管连接,移液塞2卡在出液管上。本发明构造简单,操作方便,同时可以有效避免再移液过程中挥发性液体与空气直接接触,挥发到空气中,损害人体健康。

The invention discloses a pipetting device for volatile liquids. During the experimental operation, pipetting is one of the common operations. In view of the fact that the existing pipetting device cannot avoid direct contact of volatile liquids with the outside air, a pipetting device for volatile liquids is designed, in which the pipetting plug 1 is stuck on the inlet tube, and the inlet tube and the catheter Connected by the connecting plate 1, the liquid inlet pipe and the air guide tube are at the same level, the baffle is located at the boundary between the liquid guide tube and the air guide tube, the rubber tube connects the air guide tube and the pipetting barrel, the piston rod and the piston are solidified together, and the liquid is discharged The tube is connected with the catheter through the connecting plate 2, and the pipetting plug 2 is stuck on the outlet tube. The invention has the advantages of simple structure and convenient operation, and can effectively prevent the volatile liquid from being in direct contact with the air during the re-pipetting process, volatilizing into the air and damaging human health.

Description

一种针对挥发性液体的移液装置A pipetting device for volatile liquids

技术领域technical field

本发明涉及实验器材领域,更具体地说是一种针对挥发性液体的移液装置。The invention relates to the field of experimental equipment, in particular to a pipetting device for volatile liquids.

背景技术Background technique

在实验操作过程中,移液是常见的操作之一。对于挥发性的液体,有时受到实验室自身条件限制,无法在通风厨中操作,在使用实验室常见的移液装置的时候,挥发性液体会直接与外部空气接触,挥发到空气中,使实验室的空气受到污染,甚至会损害操作人员的身体健康。例如在测试水体中叶绿素的过程中,需要加入丙酮提取叶绿素,丙酮易挥发且有一定毒性,吸入人体会对人体造成损害,如果未在通风厨中直接使用实验室常见的移液装置移取丙酮,丙酮则会挥发到空气中造成。同时,装液体的试剂瓶瓶口敞开也会有液体挥发到外部空气中,使操作者的健康受到威胁。During the experimental operation, pipetting is one of the common operations. For volatile liquids, sometimes limited by the laboratory's own conditions, it cannot be operated in a fume hood. When using a common pipetting device in the laboratory, the volatile liquid will directly contact with the outside air and volatilize into the air, making the experiment The air in the room is polluted, which may even damage the health of the operator. For example, in the process of testing chlorophyll in water, it is necessary to add acetone to extract chlorophyll. Acetone is volatile and has certain toxicity. Inhalation of the human body will cause damage to the human body. , acetone will volatilize into the air. Simultaneously, also can have the liquid to volatilize in the external air, make the operator's health threatened.

发明内容Contents of the invention

1.本发明的目的:1. Purpose of the present invention:

提供一个用于移取易挥发液体的移液装置,该移液装置构造简单,操作方便,同时可以有效避免在移液过程中易挥发性液体挥发到空气中,损害操作者的身体健康。A liquid pipetting device for pipetting volatile liquid is provided. The liquid pipetting device is simple in structure and easy to operate, and can effectively prevent the volatile liquid from volatilizing into the air during the liquid pipetting process, which will damage the health of the operator.

2.本发明的技术方案:2. Technical scheme of the present invention:

为实现上述目的,本发明,一种针对挥发性液体的移液装置,包括进液管、移液塞1、连接板1、导液管、挡板、导气管、橡皮管、移液桶、活塞杆、活塞、连接板2、出液管以及移液塞2。In order to achieve the above object, the present invention, a pipetting device for volatile liquids, includes a liquid inlet pipe, a pipetting plug 1, a connecting plate 1, a catheter, a baffle, an air guide tube, a rubber tube, a pipetting barrel, Piston rod, piston, connecting plate 2, liquid outlet pipe and pipetting plug 2.

优选的是:所述的进液管是插入待移液体的试剂瓶中的玻璃管。Preferably, the liquid inlet tube is a glass tube inserted into the reagent bottle to be pipetted.

优选的是:所述的移液塞1是主视图和侧视图均为梯形的橡胶活塞,其中在活塞中心部位有一穿孔,穿孔直径与进液管外径相同,橡胶活塞可以有不同直径尺寸大小以适应于不同的盛装液体的试剂瓶口。Preferably: the pipetting plug 1 is a trapezoidal rubber piston in both the front view and the side view, wherein there is a perforation in the center of the piston, the diameter of the perforation is the same as the outer diameter of the liquid inlet tube, and the rubber piston can have different diameters and sizes To adapt to different reagent bottle mouths for holding liquids.

优选的是:所述的连接板1是在下内表面连接进液管与导液管的半弧形连接板,这样可以有效地减小角度的突变,增加缓冲,保证液体可以平缓顺畅地从进液管进入导液管。Preferably: the connecting plate 1 is a semi-arc connecting plate connecting the liquid inlet tube and the catheter tube on the lower inner surface, which can effectively reduce the sudden change of the angle, increase buffering, and ensure that the liquid can smoothly flow from the inlet The liquid tube enters the catheter.

优选的是:所述的导液管是连接进液管与出液管和导气管,液体可以通过导液管流入出液管,导液管的上表面是水平的,下表面是倾斜的,其中与进液管相连一端直径小于与出液管相连一端的直径,这样可以减小液体流入出液管的阻力,减小液体在导液管的滞留,保证液体顺利流入出液管的同时可以减小液体损失。Preferably: the catheter is connected to the liquid inlet pipe, the liquid outlet pipe and the air guide tube, the liquid can flow into the liquid outlet pipe through the catheter tube, the upper surface of the catheter tube is horizontal, and the lower surface is inclined. The diameter of the end connected to the liquid inlet pipe is smaller than the diameter of the end connected to the liquid outlet pipe, which can reduce the resistance of the liquid flowing into the liquid outlet pipe, reduce the retention of liquid in the catheter tube, and ensure that the liquid can flow smoothly into the liquid outlet pipe. Reduce fluid loss.

优选的是:所述的挡板是位于进液管与导气管衔接的部位,与下内表面相连,其高度比导气管直径小1.5mm,可以防止液体随气流进入导气管。Preferably, the baffle is located at the joint between the liquid inlet pipe and the air guide tube, connected to the lower inner surface, and its height is 1.5mm smaller than the diameter of the air guide tube, so as to prevent the liquid from entering the air guide tube with the airflow.

优选的是:所述的导气管一端与导液管相连,另一端可以接入橡皮管。Preferably, one end of the air guiding tube is connected to the catheter, and the other end can be connected to a rubber tube.

优选的是:所述的橡皮管是橡胶材质的,可以将导气管与移液桶连接起来。Preferably: the rubber tube is made of rubber, and can connect the airway tube with the pipetting barrel.

优选的是:所述的移液桶是两端开口环形薄壁圆柱体,外表面有刻度,其中一端直径缓慢减小至与导气管直径相同,直径较小的一端与橡皮管相连,活塞从直径较大一端进入。Preferably, the pipetting barrel is an annular thin-walled cylinder with two ends open, with scales on the outer surface, the diameter of one end gradually decreases to the same diameter as the air guide tube, and the end with a smaller diameter is connected to the rubber tube, and the piston moves from The larger diameter end enters.

优选的是:所述的活塞杆是与活塞固结在一起的,外力可以借助活塞杆使活塞能够在移液桶内前后自由活动。Preferably, the piston rod is fixed together with the piston, and external force can use the piston rod to make the piston freely move back and forth in the pipetting barrel.

优选的是:所述的活塞是橡胶材质的,其直径与移液桶的内径相同,与移液桶内壁有良好的接触,可以在移液桶内前后活动。Preferably, the piston is made of rubber, its diameter is the same as the inner diameter of the pipetting barrel, it has good contact with the inner wall of the pipetting barrel, and can move back and forth in the pipetting barrel.

优选的是:所述的连接板2是在下内表面连接导液管与出液管的半弧形连接板,这样可以有效地减小角度的突变,增加缓冲,保证液体可以平缓顺畅地从导液管进入出液管。Preferably: the connecting plate 2 is a semi-arc connecting plate connecting the catheter and the outlet pipe on the lower inner surface, which can effectively reduce the sudden change of the angle, increase the buffer, and ensure that the liquid can flow smoothly from the guide to the outlet. The liquid pipe enters the liquid outlet pipe.

优选的是:所述的出液管是插入待盛装液体的实验器皿中的玻璃管。Preferably: said liquid outlet pipe is a glass pipe inserted into a laboratory vessel to be filled with liquid.

优选的是:所述的移液塞2是主视图和侧视图均为梯形的橡胶活塞,其中在活塞中心部位有一穿孔,穿孔直径与出液管直径相同,橡胶活塞可以有不同直径尺寸大小以适应于不同的待盛装液体的实验器皿口。Preferably: the pipetting plug 2 is a trapezoidal rubber piston in both the front view and the side view, wherein there is a perforation in the center of the piston, the diameter of the perforation is the same as the diameter of the liquid outlet pipe, and the rubber piston can have different diameters and sizes. It is suitable for different experimental vessel mouths to be filled with liquids.

优选的是:所述的整个装置的所有接口处均密封性良好。Preferably, all the interfaces of the whole device are well sealed.

附图说明Description of drawings

图1是装置的整体图。Fig. 1 is an overall view of the device.

图2是连接板1或连接板2的放大图。FIG. 2 is an enlarged view of the connection plate 1 or the connection plate 2 .

图3是挡板的放大图。Fig. 3 is an enlarged view of the baffle.

图4是移液塞1或移液塞2的俯视图、左视图以及主视图。FIG. 4 is a top view, a left side view and a front view of the pipetting plug 1 or the pipetting plug 2 .

具体实施方式detailed description

下面结合具体实例对本发明进一步说明。The present invention will be further described below in conjunction with specific examples.

如图1所示,一种针对挥发性液体的移液装置主要包括进液管(1)、移液塞1(2)、连接板1(3)、导液管(4)、挡板(5)、导气管(6)、橡皮管(7)、移液桶(8)、活塞杆(9)、活塞(10)、连接板2(11)、出液管(12)以及移液塞2(13)。As shown in Figure 1, a pipetting device for volatile liquids mainly includes a liquid inlet tube (1), a pipetting plug 1 (2), a connecting plate 1 (3), a catheter (4), a baffle ( 5), air guide tube (6), rubber tube (7), pipetting barrel (8), piston rod (9), piston (10), connecting plate 2 (11), liquid outlet pipe (12) and pipetting plug 2(13).

使用时先利用活塞杆(9)将活塞(10)置于移液桶(8)的最顶端,利用橡皮管(7)将导气管(6)与导液管(4)相连。During use, the piston rod (9) is used to place the piston (10) on the top of the pipetting barrel (8), and the air guide tube (6) is connected to the catheter tube (4) by the rubber tube (7).

然后将进液管(1)插入盛装液体的试剂瓶内,将移液塞1(2),如图4所示,旋转塞入试剂瓶口,确保试剂瓶内密封性良好;将出液管(12)插入待盛装液体的实验器皿中,将移液塞2(13),如图4所示,旋转塞入待盛装液体的实验器皿口,确保待盛装液体的实验器皿内密封性良好。Then insert the liquid inlet tube (1) into the reagent bottle containing the liquid, and insert the pipette plug 1 (2), as shown in Figure 4, into the mouth of the reagent bottle by rotation to ensure good sealing in the reagent bottle; (12) Insert into the experimental vessel to be filled with liquid, and insert the pipette plug 2 (13), as shown in Figure 4, into the mouth of the experimental vessel to be filled with liquid, to ensure good sealing in the experimental vessel to be filled with liquid.

最后向外拉动活塞杆(9),活塞(10)向外移动,装置内气压小于试剂瓶内气压,液体会顺着进液管(1),流经连接板1(3),如图2(1)所示,通过导液管(4),流经连接板2(11),如图2(2)所示,最后经过出液管(12)进入待盛装液体的实验器皿中。在液体流经连接板2(11)时,挡板(5),如图3所示,可以有效避免液体进入导气管(6),仅使气体进入。Finally, pull the piston rod (9) outward, the piston (10) moves outward, the air pressure in the device is lower than the air pressure in the reagent bottle, and the liquid will flow through the connecting plate 1 (3) along the liquid inlet pipe (1), as shown in Figure 2 As shown in (1), flow through the connecting plate 2 (11) through the catheter (4), as shown in Figure 2 (2), and finally enter the experimental vessel to be filled with liquid through the liquid outlet pipe (12). When the liquid flows through the connecting plate 2 (11), the baffle (5), as shown in Figure 3, can effectively prevent the liquid from entering the air guide tube (6), and only allow the gas to enter.

移液结束后,分别将移液塞1(2)以及移液塞2(13)从试剂瓶口以及待盛装液体的实验器皿口从拔出,将装置润洗后继续重复上述步骤进行移液或将橡皮管(7)与导气管(6)以及移液桶(8)的连接断开,清洗好装置后收纳。After pipetting, pull out the pipetting plug 1 (2) and pipetting plug 2 (13) from the mouth of the reagent bottle and the mouth of the experimental vessel to contain the liquid, rinse the device and continue to repeat the above steps for pipetting Or disconnect the rubber tube (7) from the air guide tube (6) and the pipette barrel (8), clean the device and put it away.

Claims (8)

1.一种针对挥发性液体的移液装置,主要包括进液管(1)、移液塞1(2)、连接板1(3)、导液管(4)、挡板(5)、导气管(6)、橡皮管(7)、移液桶(8)、活塞杆(9)、活塞(10)、连接板2(11)、出液管(12)以及移液塞2(13)。1. A pipetting device for volatile liquids, mainly comprising a liquid inlet tube (1), a pipetting plug 1 (2), a connecting plate 1 (3), a catheter (4), a baffle (5), Air guide tube (6), rubber tube (7), pipetting barrel (8), piston rod (9), piston (10), connecting plate 2 (11), liquid outlet tube (12) and pipetting plug 2 (13 ). 2.根据权利要求1所述的一种针对挥发性液体的移液装置,其特征在于所述的移液塞1(2)、移液塞2(13)是主视图和侧视图均为梯形的橡胶活塞,其中在橡胶活塞中心部位有一穿孔,橡胶活塞可以有不同直径尺寸大小以适应于不同的盛装液体的试剂瓶口或不同的待盛装液体的实验器皿口。2. A kind of pipetting device for volatile liquids according to claim 1, characterized in that said pipetting plug 1 (2), pipetting plug 2 (13) are trapezoidal in front view and side view The rubber piston has a perforation in the central part of the rubber piston, and the rubber piston can have different diameters and sizes to adapt to different reagent bottle mouths containing liquids or different experimental vessel mouths to be filled with liquids. 3.根据权利要求1所述的一种针对挥发性液体的移液装置,其特征在于所述的连接板1(3)是在下内表面连接进液管(1)与导液管(4)的半弧形连接板,连接板2(11)是在下内表面连接导液管(4)与出液管(12)的半弧形连接板。3. A pipetting device for volatile liquids according to claim 1, characterized in that the connecting plate 1 (3) is connected to the liquid inlet tube (1) and the catheter tube (4) on the lower inner surface The semi-arc connecting plate, connecting plate 2 (11) is the semi-arc connecting plate that connects the catheter tube (4) and the liquid outlet pipe (12) at the lower inner surface. 4.根据权利要求1所述的一种针对挥发性液体的移液装置,其特征在于所述的导液管(4)是连接进液管(1)与出液管(12)和导气管(6),导液管(4)的上表面是水平的,下表面是倾斜的,其中与进液管(1)相连一端直径小于与出液管(12)相连一端的直径。4. A pipetting device for volatile liquids according to claim 1, characterized in that the catheter (4) is connected to the liquid inlet pipe (1) and the liquid outlet pipe (12) and the air duct (6), the upper surface of the catheter (4) is horizontal and the lower surface is inclined, wherein the diameter of the end connected to the liquid inlet pipe (1) is smaller than the diameter of the end connected to the liquid outlet pipe (12). 5.根据权利要求1所述的一种针对挥发性液体的移液装置,其特征在于所述的挡板(5)是位于进液管(1)与导气管(6)衔接的部位,与下内表面相连,其高度比导气管(6)直径小1.5mm。5. A pipetting device for volatile liquids according to claim 1, characterized in that the baffle (5) is located at the part where the liquid inlet pipe (1) connects with the air guide pipe (6), and The lower inner surface is connected, and its height is 1.5mm smaller than the diameter of the airway tube (6). 6.根据权利要求1所述的一种针对挥发性液体的移液装置,其特征在于所述的导气管(6)一端与导液管(4)相连,另一端可以接入橡皮管(7),橡皮管(7)可以将导气管(6)与移液桶(8)连接起来。6. A pipetting device for volatile liquids according to claim 1, characterized in that one end of the air guide tube (6) is connected to the catheter tube (4), and the other end can be connected to a rubber tube (7 ), the rubber tube (7) can connect the airway (6) with the pipetting barrel (8). 7.根据权利要求1所述的一种针对挥发性液体的移液装置,其特征在于移液桶(8)是两端开口环形薄壁圆柱体,外表面有刻度,其中一端直径缓慢减小至与导气管(6)直径相同。7. A pipetting device for volatile liquids according to claim 1, characterized in that the pipetting barrel (8) is an annular thin-walled cylinder with two ends open, and a scale on the outer surface, wherein the diameter of one end decreases slowly To the same diameter as the airway tube (6). 8.根据权利要求1所述的一种针对挥发性液体的移液装置,其特征在于所述的活塞杆(9)是与活塞(10)固结在一起的,活塞(10)直径与移液桶(8)的内径相同,与移液桶(8)内壁有良好的接触,可以在移液桶(8)内前后自由活动。8. A pipetting device for volatile liquids according to claim 1, characterized in that the piston rod (9) is consolidated with the piston (10), and the diameter of the piston (10) is the same as that of the pipette. The inner diameters of the liquid barrels (8) are identical, and have good contact with the inner wall of the pipetting barrel (8), and can freely move back and forth in the pipetting barrel (8).
CN201611223023.4A 2016-12-27 2016-12-27 A kind of liquid-transfering device for volatile liquid Active CN106492896B (en)

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CN109012768A (en) * 2017-06-09 2018-12-18 国家纳米科学中心 Micro-fluidic liquid one-way flowing control structure, chip and method
CN112704491A (en) * 2020-12-28 2021-04-27 华南理工大学 Lower limb gait prediction method based on attitude sensor and dynamic capture template data

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US4759825A (en) * 1985-07-25 1988-07-26 Bela Medvey Rotary evaporator
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CN112704491A (en) * 2020-12-28 2021-04-27 华南理工大学 Lower limb gait prediction method based on attitude sensor and dynamic capture template data

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