CN106479873A - A kind of microorganism truffle punching classification inoculation apparatus - Google Patents

A kind of microorganism truffle punching classification inoculation apparatus Download PDF

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Publication number
CN106479873A
CN106479873A CN201610865118.XA CN201610865118A CN106479873A CN 106479873 A CN106479873 A CN 106479873A CN 201610865118 A CN201610865118 A CN 201610865118A CN 106479873 A CN106479873 A CN 106479873A
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CN
China
Prior art keywords
truffle
bucket body
support bar
taper bucket
microorganism
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Granted
Application number
CN201610865118.XA
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Chinese (zh)
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CN106479873B (en
Inventor
李风浪
李舒歆
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Jiangsu Hengkang Biotechnology Co., Ltd.
Original Assignee
Dongguan Lianzhou Intellectual Property Operation and Management Co Ltd
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Priority to CN201610865118.XA priority Critical patent/CN106479873B/en
Publication of CN106479873A publication Critical patent/CN106479873A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

Abstract

The classification inoculation apparatus the invention discloses a kind of microorganism truffle punches, including taper bucket body, it is fixedly connected with inclined support bar on the upper end lateral wall of taper bucket body, gathering sill is formed on described inclined support bar, it is plugged with guide pad in described gathering sill, the rear end of described guide pad is fixed with pole, described guide pad be fixedly arranged at the front end with connecting rod, the lifting rod of arc is hinged with described connecting rod, the bottom of described taper bucket body is hinged with bottom, one end both sides of described bottom are hinged in taper bucket body bottom sidewall by jointed shaft, the bottom of the other end is fixed on one end of lifting rod, guide ring is formed in the bottom sidewall of taper bucket body, the inserting groove of arc is formed on bottom, described inserting groove is plugged on described guide ring, guide ring upper sleeve has the reduction torsion spring of annular.Truffle is taken out in convenient punching in the case that the present invention is difficult to make culture dish pollution microbes, and when when mobile, truffle is difficult from card punch landing and inoculates, truffle departs from easily from card punch.

Description

A kind of microorganism truffle punching classification inoculation apparatus
Technical field:
The present invention relates to the technical field of microbial cultivation device, it is specifically related to a kind of microorganism truffle punching inoculation dress Put.
Background technology:
In the production of microorganism, scientific experiment, truffle is accessed plating medium, treat that truffle grows in the medium After certain area, become flat board containing bacterium.Now, conventional card punch takes truffle of the same size (culture) to connect from flat board containing bacterium Enter in fresh culture medium or gel.At present, when conventional use of card punch has mobile truffle easily from card punch landing, connect When planting, truffle is difficult to depart from from card punch, and speed of operation is slow, ineffective shortcoming, and in punching and inoculation, culture Ware lid opens excessive or overlong time, easily causes living contaminantses.
Content of the invention:
The purpose of the present invention aims to solve the problem that the problem that prior art exists, and provides and a kind of is difficult to make culture dish pollution microbes In the case of convenient punching take out truffle, when when mobile, truffle is difficult from card punch landing and inoculates, truffle is de- easily from card punch From microorganism truffle punching classification inoculation apparatus.
The classification inoculation apparatus the present invention relates to a kind of microorganism truffle punches, including taper bucket body, the upper end of described taper bucket body It is fixedly connected with inclined support bar on lateral wall, described inclined support bar forms gathering sill, in described gathering sill, be plugged with guiding Block, the rear end of described guide pad is fixed with pole, and the rear end of described pole forms limit shaft through locating piece, described locating piece It is fixed on the lower end of inclined support bar, pole upper sleeve has spring, the two ends of described spring press up against locating piece and guide pad On;
Described guide pad be fixedly arranged at the front end with connecting rod, described connecting rod is hinged with the lifting rod of arc, described taper The bottom of bucket body is hinged with bottom, and one end both sides of described bottom are hinged in taper bucket body bottom sidewall by jointed shaft, separately The bottom of one end is fixed on one end of lifting rod, and the bottom sidewall of taper bucket body between described jointed shaft forms guiding Ring, bottom forms the inserting groove of arc, and described inserting groove is plugged on described guide ring, and guide ring upper sleeve has annular Reduction torsion spring, one end of described reduction torsion spring is fixed on guide ring, the other end is fixed on the side wall of inserting groove.
By technique scheme, when using, the hand-held inclined support bar of operator is simultaneously for the punching classification inoculation apparatus of the present invention Pull one end of pole upper limit axle, pole moves up drive guide pad and moves up in the gathering sill of inclined support bar, bullet Spring is compressed, and pole drives connecting rod to also move up, and connecting rod drives the lifting rod of hinged arc thereon to move, lifting One end of bar drives the bottom of taper bucket body bottom to rotate around with taper bucket body hinged place, the grafting on bottom in the process Groove rotates around guide ring and compresses reduction torsion spring, so that the bottom opening of taper bucket body, then on flat board containing bacterium Push taper bucket body and realize truffle punching, above carry taper bucket body and bleed off pole after completing punching, in spring and reduction torsion spring Under dual function, connecting rod and lifting rod reset, and drive bottom to rotate around resetting with taper bucket body hinged place, reduction torsion spring is multiple Position makes inserting groove rotate on guide ring, until bottom covers in the bottom of taper bucket body, again so that truffle will not Drop out in the taper bucket body, then move to taper bucket body above fresh culture medium or gel, by inverting inclined support bar Taper bucket body is inverted, thus truffle is moved in fresh culture medium or gel.
By such scheme, the truffle punching classification inoculation apparatus of the present invention are difficult to make convenience in the case of culture dish pollution microbes Truffle is taken out in punching, and when when mobile, truffle is difficult from card punch landing and inoculates, truffle departs from easily from card punch.
A kind of preferred as such scheme, described guide pad forms two limited blocks, described two limited blocks divide It is not resisted against on the top surface and bottom surface of inclined support bar.
A kind of preferred as such scheme, the rear end of described limit shaft forms draw ring, on the medial wall of described draw ring Form cushion, draw ring is in vertically arranged, and the outer wall of draw ring is resisted against on the lower surface of inclined support bar.
A kind of preferred as such scheme, described pole is round bar, the central axis of pole and the central shaft of connecting rod Line is located along the same line, and pole and inclined support bar are parallel.
A kind of preferred as such scheme, the rear end of described inclined support bar is fixed with handle, oblique on front side of described handle Block is fixed with support bar.
A kind of preferred as such scheme, the top surface of one end that described bottom is fixedly connected with lifting rod forms bullet Property cushion.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention, And can be practiced according to the content of description, below with presently preferred embodiments of the present invention and coordinate accompanying drawing describe in detail as after.
Brief description:
The following drawings is only intended to, in doing schematic illustration and explanation to the present invention, not delimit the scope of the invention.Wherein:
Fig. 1 is the structural representation of the present invention;
Fig. 2 is the top view of Fig. 1;
Fig. 3 is the structural representation in the present invention between bottom and guide ring;
Fig. 4 is the working state schematic representation of Fig. 3;
Fig. 5 is the profile of draw ring in the present invention.
Specific embodiment:
With reference to the accompanying drawings and examples, the specific embodiment of the present invention is described in further detail.Hereinafter implement Example is used for the present invention is described, but is not limited to the scope of the present invention.
Referring to Fig. 1, Fig. 2, Fig. 5, a kind of microorganism truffle punching classification inoculation apparatus of the present invention, including taper bucket body 10, the upper end lateral wall of described taper bucket body is fixedly connected with inclined support bar 20, the rear end of described inclined support bar is fixed with handss Handle 21, the inclined support bar on front side of described handle is fixed with block 22, and inclined support bar 20 forms gathering sill 23, described guiding It is plugged with guide pad 30 in groove, described guide pad forms two limited blocks 31, described two limited blocks bear against oblique On the top surface and bottom surface of support bar 20, the rear end of guide pad 30 is fixed with pole 40, and the rear end of described pole passes through locating piece 50 Form limit shaft 41, the rear end of described limit shaft forms draw ring 42, the medial wall of described draw ring forms cushion 43, Draw ring 42 is in vertically arranged, and the outer wall of draw ring 42 is resisted against on the lower surface of inclined support bar 20, and described locating piece 50 is fixed on tiltedly The lower end of support bar 20, pole 40 upper sleeve has spring 60, and the two ends of described spring press up against locating piece 50 and guide pad On 30.
Referring to Fig. 1, Fig. 3, described guide pad 30 be fixedly arranged at the front end with connecting rod 70, described pole 40 is round bar, pole 40 Central axis and the central axis of connecting rod 70 be located along the same line, pole 40 and inclined support bar 20 are parallel, described company The lifting rod 80 of arc is hinged with extension bar 70, the bottom of described taper bucket body 10 is hinged with bottom 90, one end of described bottom Both sides are hinged in taper bucket body 10 bottom sidewall by jointed shaft 11, the bottom of the other end is fixed on one end of lifting rod 80 On, the top surface of one end that bottom 90 is fixedly connected with lifting rod 80 forms elastic cord breaker 91, between described jointed shaft 11 The bottom sidewall of taper bucket body 10 on form guide ring 12, bottom 90 forms the inserting groove 92 of arc, described grafting Groove is plugged on described guide ring 12, and guide ring upper sleeve has the reduction torsion spring 100 of annular, and one end of described reduction torsion spring is fixed On guide ring 12, the other end be fixed on inserting groove 92 side wall on.
Referring to Fig. 4, in the specific implementation, the hand-held inclined support bar of operator 20 simultaneously pulls the punching classification inoculation apparatus of the present invention One end of pole 40 upper limit axle 41, pole 40 move up drive guide pad 30 in the gathering sill 23 of inclined support bar 20 upwards Mobile, spring 60 is compressed, and pole 40 drives connecting rod 70 to also move up, and connecting rod 70 drives hinged arc thereon Lifting rod 80 is mobile, and one end of lifting rod 80 drives the bottom 90 of taper bucket body 10 bottom to turn around with taper bucket body 10 hinged place Dynamic, the inserting groove 92 on bottom 90 rotates around guide ring 12 and compresses reduction torsion spring 100 in the process, so that cone The bottom opening of shape bucket body 10, then pushes the taper bucket body 10 on flat board containing bacterium and realizes truffle punching, above carry after completing punching Taper bucket body 10 simultaneously bleeds off pole 40, and under the dual function of spring 60 and reduction torsion spring 100, connecting rod 70 and lifting rod 80 are multiple Position, drives bottom 90 to rotate around resetting with taper bucket body 10 hinged place, and reduction torsion spring 100 resets and makes inserting groove 92 in guiding Rotate on ring 12, until bottom 90 covers in the bottom of taper bucket body 10, again so that truffle will not be from taper bucket body 10 Inside drop out, then move to taper bucket body 10 above fresh culture medium or gel, by inverting inclined support bar 20 by taper Bucket body 10 is inverted, thus truffle is moved in fresh culture medium or gel.
In sum, the truffle punching classification inoculation apparatus of the present invention are difficult to make convenient punching in the case of culture dish pollution microbes Take out truffle, when truffle is difficult from card punch landing and inoculates when mobile, truffle departs from easily from card punch.
Microorganism truffle punching classification inoculation apparatus provided by the present invention, the only specific embodiment of the present invention, but this Bright protection domain is not limited thereto, any those familiar with the art the invention discloses technical scope Interior, change or replacement can be readily occurred in, all should cover within the scope of the present invention.Therefore, protection scope of the present invention should It is defined by described scope of the claims.

Claims (6)

1. a kind of microorganism truffle punching classification inoculation apparatus it is characterised in that:
Including taper bucket body (10), the upper end lateral wall of described taper bucket body is fixedly connected with inclined support bar (20), described oblique Gathering sill (23) is formed on support bar, in described gathering sill, is plugged with guide pad (30), the rear end of described guide pad is fixed with Pole (40), the rear end of described pole forms limit shaft (41) through locating piece (50), and described locating piece (50) is fixed on tiltedly The lower end of support bar (20), pole (40) upper sleeve has spring (60), and the two ends of described spring press up against locating piece (50) On guide pad (30);
The lifting rod (80) being fixedly arranged at the front end with connecting rod (70), described connecting rod being hinged with arc of described guide pad (30), The bottom of described taper bucket body (10) is hinged with bottom (90), and one end both sides of described bottom are hinged on cone by jointed shaft (11) In shape bucket body (10) bottom sidewall, the bottom of the other end is fixed on one end of lifting rod (80), between described jointed shaft (11) The bottom sidewall of taper bucket body (10) on form guide ring (12), bottom (90) forms the inserting groove (92) of arc, Described inserting groove is plugged on described guide ring (12), and guide ring (12) upper sleeve has the reduction torsion spring (100) of annular, described multiple One end of position torsion spring is fixed on guide ring (12), the other end is fixed on the side wall of inserting groove (92).
2. microorganism truffle according to claim 1 punching classification inoculation apparatus it is characterised in that:Become on described guide pad (30) Type has two limited blocks (31), and described two limited blocks bear against on the top surface and bottom surface of inclined support bar (20).
3. microorganism truffle according to claim 1 punching classification inoculation apparatus it is characterised in that:After described limit shaft (41) End forms draw ring (42), and the medial wall of described draw ring forms cushion (43), and draw ring (42) is in vertically arranged, draw ring (42) outer wall is resisted against on the lower surface of inclined support bar (20).
4. microorganism truffle according to claim 1 punching classification inoculation apparatus it is characterised in that:Described pole (40) is circle Bar, the central axis of the central axis of pole (40) and connecting rod (70) is located along the same line, pole (40) and inclined support bar (20) parallel.
5. microorganism truffle according to claim 1 punching classification inoculation apparatus it is characterised in that:Described inclined support bar (20) Rear end is fixed with handle (21), and the inclined support bar (20) on front side of described handle is fixed with block (22).
6. microorganism truffle according to claim 1 punching classification inoculation apparatus it is characterised in that:Described bottom (90) and lifting Elastic cord breaker (91) is formed on the top surface of one end that bar (80) is fixedly connected.
CN201610865118.XA 2016-09-25 2016-09-25 A kind of microorganism fungus block punching classification inoculation apparatus Active CN106479873B (en)

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CN201610865118.XA CN106479873B (en) 2016-09-25 2016-09-25 A kind of microorganism fungus block punching classification inoculation apparatus

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4234023A (en) * 1977-03-02 1980-11-18 Olympus Optical Co., Ltd. Liquid feeder for automatic culture apparatus
JPH08256757A (en) * 1995-03-02 1996-10-08 Batec Bio Analitical Technol Ltd Apparatus for detecting microorganism in sample
US5900373A (en) * 1996-04-23 1999-05-04 Iul, S.A. System of inoculation of biological samples onto the agar surface in Petri capsules
CN202374741U (en) * 2011-12-31 2012-08-15 佳木斯大学 Bacteria piece inoculator
CN202610233U (en) * 2012-05-28 2012-12-19 牡丹江师范学院 Microorganism flat plate hole puncher
CN204147893U (en) * 2014-10-25 2015-02-11 袁兰英 A kind of Physical Experiment medication spoon
CN204298403U (en) * 2014-10-21 2015-04-29 中国热带农业科学院南亚热带作物研究所 A kind of bacterium dish is beaten and is got device
CN205258470U (en) * 2015-12-25 2016-05-25 青岛市第三人民医院 Bacterium examination sampling ware

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4234023A (en) * 1977-03-02 1980-11-18 Olympus Optical Co., Ltd. Liquid feeder for automatic culture apparatus
JPH08256757A (en) * 1995-03-02 1996-10-08 Batec Bio Analitical Technol Ltd Apparatus for detecting microorganism in sample
US5900373A (en) * 1996-04-23 1999-05-04 Iul, S.A. System of inoculation of biological samples onto the agar surface in Petri capsules
CN202374741U (en) * 2011-12-31 2012-08-15 佳木斯大学 Bacteria piece inoculator
CN202610233U (en) * 2012-05-28 2012-12-19 牡丹江师范学院 Microorganism flat plate hole puncher
CN204298403U (en) * 2014-10-21 2015-04-29 中国热带农业科学院南亚热带作物研究所 A kind of bacterium dish is beaten and is got device
CN204147893U (en) * 2014-10-25 2015-02-11 袁兰英 A kind of Physical Experiment medication spoon
CN205258470U (en) * 2015-12-25 2016-05-25 青岛市第三人民医院 Bacterium examination sampling ware

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Applicant before: Dongguan Lianzhou Intellectual Property Operation Management Co.,Ltd.

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