CN106390911B - The method for preparing the method for pelelith adsorbent and being enriched with catechin antioxidant - Google Patents
The method for preparing the method for pelelith adsorbent and being enriched with catechin antioxidant Download PDFInfo
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- CN106390911B CN106390911B CN201610703128.3A CN201610703128A CN106390911B CN 106390911 B CN106390911 B CN 106390911B CN 201610703128 A CN201610703128 A CN 201610703128A CN 106390911 B CN106390911 B CN 106390911B
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
- B01J20/103—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate comprising silica
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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- B01J2220/40—Aspects relating to the composition of sorbent or filter aid materials
- B01J2220/48—Sorbents characterised by the starting material used for their preparation
- B01J2220/4806—Sorbents characterised by the starting material used for their preparation the starting material being of inorganic character
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Abstract
The present invention provides a kind of method for preparing pelelith adsorbent and the method for being enriched with catechin antioxidant, and the method for preparing pelelith adsorbent includes the following steps: to provide 24 mesh~40 mesh pelelith particles；It is impregnated the pelelith particle some time using aqueous citric acid solution, and rinses and dry；Pelelith particle after being dried using alcohol solution dipping, and rinse and dry, obtain graininess pelelith adsorbent.It is an advantage of the current invention that porous and scleroid feature makes adsorbent using natural pelelith, poisonous and harmful chemical solvent is not used, adsorption column is not easy to be blocked, and absorption and elution efficiency are high.
Description
Technical field
The present invention relates to natural separation preparation field more particularly to a kind of methods for preparing pelelith adsorbent
And the method for enrichment catechin antioxidant.
Background technique
Catechin antioxidant is that the one group of polyphenol compound obtained is extracted from tealeaves, has good antioxygen
Change, is anti-radiation, preventing a variety of physiological active functions such as cardiovascular and cerebrovascular disease and prevention cancerous swelling, being widely used in functional food and guarantor
In strong drug.
The preparation of tea catechin class antioxidant generally uses calcium ions precipitate, organic solvent separation, overcritical dioxy
Change the methods of carbon extraction or column chromatography for separation.But every kind of method all have the defects that it is certain, for example, calcium precipitation be easy
Catechin is caused to aoxidize, purity reduces；There are chemical residues for Batteries by Organic Solvent Partition Process；Supercritical carbon dioxide extraction method at
This height；There are chemical residuals when in column chromatography for separation method using chemical synthesis resin as adsorbent, and using lignocellulosic is to inhale
It because quality is soft is easy that chromatographic column blocking is made to lead to problems such as elution efficiency low at attached dose.
Therefore, a kind of method of new enrichment catechin antioxidant is needed.
Summary of the invention
The technical problem to be solved by the invention is to provide a kind of method for preparing pelelith adsorbent and enrichment catechins
The method of class antioxidant can obtain the catechin antioxidant of no chemical residues, and adsorb high with elution efficiency.
To solve the above-mentioned problems, the present invention provides a kind of method for preparing pelelith adsorbent, include the following steps:
24 mesh~40 mesh pelelith particles are provided；
It is impregnated the pelelith particle some time using aqueous citric acid solution, and rinses and dry；
Pelelith particle after being dried using alcohol solution dipping, and rinse and dry, obtain the absorption of graininess pelelith
Agent.
Further, 24 mesh~40 mesh pelelith particles successively pass through coarse crushing, fine crushing and sieving and obtain.
Further, the aqueous citric acid solution submerges the pelelith particle, impregnates the fire using aqueous citric acid solution
The time of mountain stone particle is 12 hours~24 hours.
Further, the ethanol solution submerges the pelelith particle.
Further, the concentration of the ethanol solution is 50%~90%, the pelelith after being dried using alcohol solution dipping
The time of particle is 12 hours~24 hours.
Further, in the baking step carried out respectively after aqueous citric acid solution and alcohol solution dipping, drying temperature is
100~110 DEG C.
The present invention also provides a kind of methods for being enriched with catechin antioxidant, include the following steps:
Millet paste is provided；
Pelelith adsorbent prepared by above-mentioned preparation method is packed into adsorption column；
The millet paste is flowed through the adsorption column to adsorb；
The adsorption column is eluted with ethanol solution, and collects the ethanol solution after elution, obtains and contains catechin antioxygen
The ethanol solution of agent；
The ethanol solution containing catechin antioxidant is concentrated, and is dried, it is anti-oxidant to obtain catechin
Agent.
Further, the millet paste volume is 3~10 times of the absorption column volume.
Further, after the millet paste flows through the adsorption column, the adsorption column is rinsed with pure water, until non-pigment
Outflow.
Further, it is eluted in the adsorption column step with ethanol solution, the concentration of the ethanol solution is 50%~95%.
Further, in the step of ethanol solution containing catechin antioxidant is concentrated, catechin will be contained
The ethanol solution rotary evaporation inspissator of class antioxidant is concentrated under reduced pressure into solid concentration 30%~50% at 40-45 DEG C.
It is an advantage of the current invention that porous and scleroid feature makes adsorbent using natural pelelith, do not use
Poisonous and harmful chemical solvent, adsorption column are not easy to be blocked, and absorption and elution efficiency are high.
Specific embodiment
Below to the method provided by the invention for preparing pelelith adsorbent and the method for being enriched with catechin antioxidant
Specific embodiment elaborate.
A kind of method for preparing pelelith adsorbent of the present invention, includes the following steps:
(1) 24 mesh~40 mesh pelelith particles are provided.
Obtain 24 mesh~40 mesh pelelith particles method are as follows:
(10) pelelith described in coarse crushing, for example, smashing bulk pelelith to form 1cm~3cm crushed stone using iron hammer.
(11) pelelith described in fine crushing, for example, 1cm~3cm pelelith crushed stone is crushed granulating using pulverizer
Spend smaller pelelith particle or powder.
(12) it is sieved, for example, with the successive sub-sieve pelelith particle of sieve and powder of 24 mesh and 40 mesh, takes on 24 mesh, 40
Now 24 mesh~40 mesh pelelith particles are used for subsequent technique.
(2) it is impregnated the 24 mesh~40 mesh pelelith particle some times using aqueous citric acid solution, and rinses and dry.
The concentration of the aqueous citric acid solution is 10g/L~20g/L, and dosage is that aqueous citric acid solution submerges pelelith
Grain, for example, the volume ratio of the volume of the aqueous citric acid solution and the pelelith particle can for 1:1,2:1,3:1,
4:1,5:1 etc., soaking time is 12~for 24 hours, such as 12 hours, 14 hours, 16 hours, 18 hours, 20 hours, 22 hours, 24
Hour.After immersion, aqueous citric acid solution is removed with filtered through gauze, with distilled water flushing pelelith particle to non-pigment stream
Out, it is dried at 100~110 DEG C.
(3) it using the pelelith particle after alcohol solution dipping drying, and rinses and dries, obtain the suction of graininess pelelith
Attached dose.
The concentration of the ethanol solution is 50%~90%, for example, 50%, 60%, 70%, 80%, 90%, using ethyl alcohol
The time that solution impregnates the pelelith particle after drying is 12 hours~24 hours, such as 12 hours, 14 hours, 16 hours, 18
Hour, 20 hours, 22 hours, 24 hours.Preferably, ethanol solution just submerges the pelelith particulate matter.After immersion,
Ethanol solution is removed with filtered through gauze, is flowed out with distilled water flushing pelelith particle to non-pigment, dries, obtains at 100~110 DEG C
To graininess pelelith adsorbent.
Pelelith is a kind of porous, lightweight nature of glass acidity volcano effusive rock, and chemical component is mainly SiO2、Al2O3
And the transition metal oxide containing there are many, have the characteristics that large specific surface area, light weight, quality are hard, anticorrosive.Benefit of the invention
With natural pelelith, porous and scleroid feature makes adsorbent, does not use poisonous and harmful chemical solvent, and adsorption column is not allowed
It is easily blocked, absorption and elution efficiency are high.
The present invention also provides a kind of methods for being enriched with catechin antioxidant, include the following steps:
(1) millet paste is provided.
The acquisition methods of the millet paste are as follows: by dry tealeaves, such as green tea, with the extracting in boiling water of 10~20 times of weight
30~60min；Tea grounds is removed with filtered through gauze, millet paste is collected and is cooled to room temperature；By millet paste under 4000~10000rpm revolving speed
It is centrifuged 15~30min, takes millet paste supernatant as millet paste in subsequent enrichment catechin antioxidant technique.
(2) the pelelith adsorbent by the method preparation for being prepared pelelith adsorbent using the present invention is fitted into adsorption column.
(3) millet paste adsorption column is flowed through to adsorb.
The volume of the millet paste be adsorb 3-10 times of column volume, for example, 3 times, 4 times, 5 times, 6 times, 7 times, 8 times, 9 times,
10 times.Further, after the millet paste all flows through adsorption column, the adsorbate is cleaned to non-pigment stream with pure water substitution millet paste
Out.
(4) adsorption column is eluted with ethanol solution, and collects the ethanol solution after elution, obtained anti-containing catechin
The ethanol solution of oxidant.
Preferably, the concentration of the ethanol solution be 50%~95%, for example, 50%, 60%, 70%, 80%, 90%,
95%.
(5) ethanol solution containing catechin antioxidant is concentrated, and is dried, obtains catechin antioxygen
Agent.
The method that the ethanol solution containing catechin antioxidant is concentrated is that will contain catechin antioxidant
Ethanol solution rotary evaporation inspissator solid concentration 30%~50% is concentrated under reduced pressure at 40-45 DEG C.Object after concentration
Powdered catechin antioxidant is obtained after matter is spray-dried.With the tea catechin class for the method enrichment that the present invention is enriched with
The catechin total content of antioxidant is 758.03~802.84mg/g.
Several embodiments of the method for present invention enrichment catechin antioxidant are set forth below, to further illustrate this hair
Bright advantage.
Embodiment 1: a method of catechin antioxidant is enriched with from steaming green tea soup.
(1) prepared by adsorbent
3 pieces of natural pelelith (5.6kg) is taken, is smashed with iron hammer to the crushed stone of 1cm~3cm；It will be fiery with pulverizer
Mountain stone is ground into powder；With 24 mesh sieve sub-sieves；The derrick stone of compass screen surface is continued to crush until all crossing 24 meshes；With 40 mesh
Screening sieve, for removal granularity less than 40 mesh powders, acquisition 4.8kg granularity is 24 mesh~40 mesh pelelith particle.
Pelelith particle is impregnated with aqueous citric acid solution (concentration 10g/L), is just submerged for aqueous citric acid solution dosage
Subject to pelelith particle, soaking time is for 24 hours；Aqueous citric acid solution is removed with filtered through gauze, with distilled water flushing volcano stone flour
It flows out to non-pigment, is dried at 100~110 DEG C.
The pelelith particle of drying is immersed in 90% ethanol solution, ethanol solution dosage is just to submerge pelelith
Subject to particle；Soaking time 20h；Ethanol solution is removed with filtered through gauze, with distilled water flushing pelelith particle to non-pigment stream
Until out, dried at 100~110 DEG C.The adsorbent that dry pelelith particle is chromatographed as column is used.
(2) prepared by millet paste
It takes steaming green tea 1500g to extract 60min with 15L boiling water, removes tea grounds with filtered through gauze, collect millet paste and be cooled to
Room temperature；Millet paste is centrifuged 30min under 4000rpm revolving speed, obtains supernatant 11.5L, for the absorption point of catechin antioxidant
From and enrichment.
(3) absorption method is enriched with millet paste catechin antioxidant
The pelelith granule adsorbent that step (1) is obtained is packed into adsorption column (diameter 7.0cm, high 95cm)；By step (2)
Whole millet paste supernatants of acquisition pour into from adsorption column top and flow through the adsorbent of adsorption column；All pass through to millet paste solution
Afterwards, it is poured into above adsorption column with 10.0L pure water and flows through adsorption column and cleaned, when efflux non-pigment, use 10.0L
95% ethanol solution elutes adsorption column, collects the ethanol solution flowed out below adsorption column, obtains and contains catechin antioxidant
Ethanol solution (9.5L)；Ethanol solution is transferred to rotary evaporator and is concentrated into about 420mL in 40-45 DEG C of reduction vaporization, together
Mini Spray-Drier spray drying, obtains 105.6g catechin antioxidant powder.
The sample of 2 repetition tests is detected through HPLC, and the catechin of the powdered catechin antioxidant of acquisition is total
Content is 802.84 ± 20.51mg/g (table 1).
The powdered catechin antioxidant catechin content (mg/g) that 1. embodiment 1 of table obtains
Wherein, SD:Standard deviation (standard deviation)；GC:gallocatechin (gallic acid catechu
Element)；EGC:epigallocatechin (epi-nutgall acid catechin)；C:catechin (catechuic acid)；EC:epicatechin
(epicatechin)；EGCG:epigallocatechin gallate (epigallocatechin gallate)；GCG:
Gallocatechin gallate (gallate-based catechin and gallate)；ECG:epicatechin gallate (table catechu
Plain gallate)；CG:catechin gallate (catechin and gallate).
Embodiment 2: a method of catechin antioxidant is enriched with from baked green tea soup.
(1) prepared by adsorbent
2 pieces of pelelith (5.2kg) are taken, is smashed with iron hammer to the crushed stone of 1cm~3cm；With pulverizer by pelelith
Block is ground into particle and powder, with 24 mesh sieve sub-sieves, the derrick stone of compass screen surface is continued to crush until all crossing 24 meshes；With 40
Mesh sub-sieve passes through the particle of 24 mesh, removes the powder at 40 mesh bottoms, and acquisition 4.3kg granularity is 24 mesh~40 mesh pelelith
Grain.
Pelelith particle is impregnated with aqueous citric acid solution (15g/L), dosage just submerges volcano with aqueous citric acid solution
Subject to stone particle, 20h is kept；Aqueous citric acid solution is removed with filtered through gauze, with distilled water flushing volcano stone flour to non-pigment stream
Out, it is dried at 100~110 DEG C.
The pelelith particle of drying is immersed in 70% ethanol solution, ethanol solution dosage is just to submerge pelelith
Subject to particle；Soaking time is for 24 hours；Ethanol solution is removed with filtered through gauze, with distilled water flushing pelelith particle to non-pigment stream
Until out, dried at 100~110 DEG C.The adsorbent that dry pelelith particle is chromatographed as column is used.
(2) prepared by millet paste
It takes baked green tea 1500g to extract 30min with 30L boiling water, removes tea grounds with filtered through gauze, collect millet paste and be cooled to
Room temperature；Millet paste is centrifuged 15min in 10000rpm, millet paste supernatant 23.7L is obtained, is used for catechin antioxidant adsorbing separation
And enrichment.
(3) absorption method is enriched with millet paste catechin antioxidant
The graininess pelelith adsorbent that step (1) is obtained is packed into adsorption column (diameter 7.0cm, high 95cm)；By step
(2) the whole millet paste supernatants obtained pour into from adsorption column top and flow through the adsorbent of adsorption column；It is all logical to millet paste solution
Later, it is poured into above adsorption column with 10.0L pure water and flows through adsorption column and cleaned, when efflux non-pigment, used
75% ethanol solution of 10.0L elutes adsorption column, collects the ethanol solution flowed out below adsorption column, obtains anti-containing catechin
The ethanol solution (9.8L) of oxidant；Ethanol solution is transferred to rotary evaporator to be concentrated into about in 40-45 DEG C of reduction vaporization
440mL is spray-dried with Mini Spray-Drier, obtains 123.4g catechin antioxidant powder.
The sample of 2 repetition tests is detected through HPLC, and the catechin of the powdered catechin antioxidant of acquisition is total
Content is 758.03 ± 4.78mg/g (table 2).
The powdered catechin antioxidant catechin content (mg/g) that 2. embodiment 2 of table obtains
Wherein, SD:Standard deviation (standard deviation)；GC:gallocatechin (gallic acid catechu
Element)；EGC:epigallocatechin (epi-nutgall acid catechin)；C:catechin (catechuic acid)；EC:epicatechin
(epicatechin)；EGCG:epigallocatechin gallate (epigallocatechin gallate)；GCG:
Gallocatechin gallate (gallate-based catechin and gallate)；ECG:epicatechin gallate (table catechu
Plain gallate)；CG:catechin gallate (catechin and gallate).
Embodiment 3: a method of catechin antioxidant is enriched with from roasted green tea soup
(1) prepared by adsorbent
3 pieces of pelelith (6.2kg) are taken, is smashed with iron hammer to the crushed stone of 1cm~3cm, with pulverizer by pelelith
Block is ground into powder, with 24 mesh sieve sub-sieves, the derrick stone of compass screen surface is continued to crush until all crossing 24 meshes；With 40 meshes point
Sieve passes through the particle of 24 mesh, removes the powder at 40 mesh bottoms, and acquisition 5.3kg granularity is 24 mesh~40 mesh graininess pelelith.
Pelelith particle is impregnated with aqueous citric acid solution (10g/L), dosage just submerges volcano with aqueous citric acid solution
Subject to stone particle, 12h is kept；Aqueous citric acid solution is removed with filtered through gauze, with distilled water flushing pelelith particulate matter to colourless
Until element outflow, dried at 100~110 DEG C.
The pelelith particle of drying is immersed in 50% ethanol solution, ethanol solution dosage is just to submerge pelelith
Subject to particle；Soaking time 12h；Ethanol solution is removed with filtered through gauze, with distilled water flushing pelelith particle to non-pigment stream
Until out, dried at 100~110 DEG C.The adsorbent that dry pelelith particle is chromatographed as column is used.
(2) prepared by millet paste
It takes roasted green tea 1500g to extract 45min with 20L boiling water, removes tea grounds with filtered through gauze, collect millet paste and be cooled to room
Temperature；Millet paste is centrifuged 20min in 8000rpm, supernatant 14.6L is obtained, is used for catechin antioxidant adsorbing separation and enrichment.
(3) absorption method is enriched with millet paste catechin antioxidant
The pelelith granule adsorbent that step (1) is obtained is packed into adsorption column (diameter 7.0cm, high 95cm)；By step (2)
The 2 whole millet paste supernatants obtained pour into from adsorption column top and flow through the adsorbent of adsorption column；All pass through to millet paste solution
Afterwards, it is poured into above adsorption column with 10.0L pure water and flows through adsorption column and cleaned, when efflux non-pigment, use 10.0L
50% ethanol solution elutes adsorption column, collects the ethanol solution flowed out below adsorption column, obtains and contains catechin antioxidant
Ethanol solution (10.2L)；Ethanol solution is transferred to rotary evaporator and is concentrated into about 480mL in 40-45 DEG C of reduction vaporization, is used
Mini Spray-Drier spray drying, obtains the powdered catechin antioxidant of 102.8g.
The sample of 2 repetition tests is detected through HPLC, and the catechin of the powdered catechin antioxidant of acquisition is total
Content is 773.19 ± 2.39mg/g (table 3).
The powdered catechin antioxidant catechin content (mg/g) that 3. embodiment 3 of table obtains
Wherein, SD:Standard deviation (standard deviation)；GC:gallocatechin (gallic acid catechu
Element)；EGC:epigallocatechin (epi-nutgall acid catechin)；C:catechin (catechuic acid)；EC:epicatechin
(epicatechin)；EGCG:epigallocatechin gallate (epigallocatechin gallate)；GCG:
Gallocatechin gallate (gallate-based catechin and gallate)；ECG:epicatechin gallate (table catechu
Plain gallate)；CG:catechin gallate (catechin and gallate).
Embodiment 4: a method of catechin antioxidant is enriched with from steaming green tea dust tea soup
(1) prepared by adsorbent
2 pieces of pelelith (5.5kg) are taken, is smashed with iron hammer to the crushed stone of 1cm~3cm, with pulverizer by pelelith
Block is ground into powder, with 24 mesh sieve sub-sieves, the derrick stone of compass screen surface is continued to crush until all crossing 24 meshes；With 40 meshes point
Sieve passes through the particle of 24 mesh, removes the powder at 40 mesh bottoms, and acquisition 4.3kg granularity is 24 mesh~40 mesh graininess pelelith.
Pelelith particle is impregnated with aqueous citric acid solution (20g/L), dosage just submerges volcano with aqueous citric acid solution
Subject to stone particle, 18h is kept；Aqueous citric acid solution is removed with filtered through gauze, with distilled water flushing pelelith particulate matter to colourless
Until element outflow, dried at 100~110 DEG C.
The pelelith particle of drying is immersed in 60% ethanol solution, ethanol solution dosage is just to submerge pelelith
Subject to particle；Soaking time 18h；Ethanol solution is removed with filtered through gauze, with distilled water flushing pelelith particle to non-pigment stream
Until out, dried at 100~110 DEG C.The adsorbent that dry pelelith particle is chromatographed as column is used.
(2) prepared by millet paste
Steaming green tea end (- 80 mesh of 60 mesh of the granular size) 1500g for taking steaming green tea process to generate is extracted with 30L boiling water
35min is taken, removes tea grounds with filtered through gauze, millet paste is collected and is cooled to room temperature；Millet paste is centrifuged 20min in 10000rpm, is obtained
Clear liquid 20.7L is used for catechin antioxidant adsorbing separation and enrichment.
(3) absorption method is enriched with millet paste catechin antioxidant
The pelelith granule adsorbent that step (1) is obtained is packed into adsorption column (diameter 7.0cm, high 95cm)；By step (2)
The 2 whole millet paste supernatants obtained pour into from adsorption column top and flow through the adsorbent of adsorption column；All pass through to millet paste solution
Afterwards, it is poured into above adsorption column with 10.0L pure water and flows through adsorption column and cleaned, when efflux non-pigment, use 10.0L
75% ethanol solution elutes adsorption column, collects the ethanol solution flowed out below adsorption column, obtains and contains catechin antioxidant
Ethanol solution (9.3L)；Ethanol solution is transferred to rotary evaporator and is concentrated into about 460mL in 40-45 DEG C of reduction vaporization, is used
Mini Spray-Drier spray drying, obtains the powdered catechin antioxidant of 109.4g.
The sample of 2 repetition tests is detected through HPLC, and the catechin of the powdered catechin antioxidant of acquisition is total
Content is 773.19 ± 2.39mg/g (table 4).
The powdered catechin antioxidant catechin content (mg/g) that 4. embodiment 4 of table obtains
Wherein, SD:Standard deviation (standard deviation)；GC:gallocatechin (gallic acid catechu
Element)；EGC:epigallocatechin (epi-nutgall acid catechin)；C:catechin (catechuic acid)；EC:epicatechin
(epicatechin)；EGCG:epigallocatechin gallate (epigallocatechin gallate)；GCG:
Gallocatechin gallate (gallate-based catechin and gallate)；ECG:epicatechin gallate (table catechu
Plain gallate)；CG:catechin gallate (catechin and gallate).
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (9)
1. a kind of method for being enriched with catechin antioxidant, which comprises the steps of:
Millet paste is provided；
Pelelith adsorbent is packed into adsorption column；
The millet paste is flowed through the adsorption column to adsorb；
The adsorption column is eluted with ethanol solution, and collects the ethanol solution after elution, obtains and contains catechin antioxidant
Ethanol solution；
The ethanol solution containing catechin antioxidant is concentrated, and is dried, obtains catechin antioxidant；Its
In, the preparation method of the pelelith adsorbent includes:
24 mesh~40 mesh pelelith particles are provided；
It is impregnated the pelelith particle some time using aqueous citric acid solution, and rinses and dry；
Pelelith particle after being dried using alcohol solution dipping, and rinse and dry, obtain graininess pelelith adsorbent.
2. the method for enrichment catechin antioxidant according to claim 1, which is characterized in that inhaled in the pelelith
In attached dose of preparation method, 24 mesh~40 mesh pelelith particles successively pass through coarse crushing, fine crushing and sieving and obtain.
3. the method for enrichment catechin antioxidant according to claim 1, which is characterized in that inhaled in the pelelith
In attached dose of preparation method, the aqueous citric acid solution submerges the pelelith particle, impregnated using aqueous citric acid solution described in
The time of pelelith particle is 12 hours~24 hours.
4. the method for enrichment catechin antioxidant according to claim 1, which is characterized in that inhaled in the pelelith
In attached dose of preparation method, the ethanol solution submerges the pelelith particle, and the concentration of the ethanol solution is 50%~
90%, the time of the pelelith particle after using alcohol solution dipping to dry is 12 hours~24 hour.
5. the method for enrichment catechin antioxidant according to claim 1, which is characterized in that inhaled in the pelelith
In attached dose of preparation method, in the baking step that is carried out respectively after aqueous citric acid solution and alcohol solution dipping, drying temperature
It is 100~110 DEG C.
6. the method for enrichment catechin antioxidant according to claim 1, which is characterized in that the millet paste volume is
3~10 times of the absorption column volume.
7. the method for enrichment catechin antioxidant according to claim 6, which is characterized in that flowed through in the millet paste
After the adsorption column, the adsorption column is rinsed, until non-pigment flows out.
8. the method for enrichment catechin antioxidant according to claim 1, which is characterized in that eluted with ethanol solution
In the adsorption column step, the concentration of the ethanol solution is 50%~95%.
9. the method for enrichment catechin antioxidant according to claim 1, which is characterized in that contain described in the concentration
In the step of ethanol solution of catechin antioxidant, by the ethanol solution rotary evaporation containing catechin antioxidant
Inspissator is concentrated under reduced pressure into solid concentration 30%~50% at 40-45 DEG C.
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