CN106383104A - Anti-interference rapid detection kit for bioluminescence microbial population - Google Patents
Anti-interference rapid detection kit for bioluminescence microbial population Download PDFInfo
- Publication number
- CN106383104A CN106383104A CN201610974744.2A CN201610974744A CN106383104A CN 106383104 A CN106383104 A CN 106383104A CN 201610974744 A CN201610974744 A CN 201610974744A CN 106383104 A CN106383104 A CN 106383104A
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- China
- Prior art keywords
- culture medium
- fluorescin
- kit
- fixed
- box body
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
Abstract
The invention discloses an anti-interference rapid detection kit for bioluminescence microbial population. The kit comprises a test reagent box, a fluorescent protein culture medium and a luminescence detection buffer reservoir, wherein the fluorescent protein culture medium is fixedly arranged inside the test reagent box; the luminescence detection buffer reservoir is fixedly arranged on one side of the fluorescent protein culture medium; a blue light spectrometer and a high performance liquid chromatograph are fixedly arranged on the edge of the test reagent box; an inductor and alarm lights are arranged on the high performance liquid chromatograph; the alarm lights are provided with red light bulbs and just arranged at two ends of the luminescence detection buffer reservoir; a dropper, a capillary pipet and a stirring rod are fixedly arranged on one section of the test reagent box through a groove; and the fluorescent protein culture medium and the luminescence detection buffer reservoir are transparent vessels. The anti-interference rapid detection kit for bioluminescence microbial population is simple in microbial detection means and simple in process.
Description
Technical field
The present invention relates to technical field, specially a kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method.
Background technology
In the past, as the microbial detection device of the detection of the microorganism carrying out in sample, it is known to use make use of three phosphorus
The ATP method of adenosine monophosphate (calling " ATP " in the following text) carries out the device of the counting of microorganism.
The ATP extracting solution containing ATP that the microorganism from sample is extracted is made and made to this microbial detection device
The structure that luminous intensity when being reacted with the ATP luminescence reagent in reaction vessel is correspondingly counted to microorganism.For example
In the method for counting microorganism trapping being counted according to the colony counting of the microorganism cultivated by plating medium,
Need the situation of several days time by the end of obtaining its result, according to such microbial detection device, the review time can be contracted
Within being short to a few houres.
Conventional kit for anti-interference quick detection of microbe quantity is in detection microorganism not intuitively as a result, it is desirable to warp
The measurement crossed again reaches and measures the effect that has or not, and detects and measure and be not bound with micro organism quantity, and causing need not
The operation wanted, wastes the time of staff.
Content of the invention
It is an object of the invention to provide a kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method, possess straight
The result drawing microorganism presence or absence seen, solves the problems, such as that conventional detection process is loaded down with trivial details.
For achieving the above object, the present invention provides following technical scheme：
A kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method, including test agent box body, fluorescin culture medium
With luminous detection buffer pool, described test agent tray interior is fixed with fluorescin culture medium, described fluorescin culture
The side of base is fixed with luminous detection buffer pool, and the edge of described test agent box body is fixed with blue light photometer and height
Effect liquid phase chromatogram instrument, described high performance liquid chromatograph is provided with induction apparatuss and alarm lamp, and described alarm lamp is provided with red globe lamp
Bubble, is just arranged on the two ends of luminous detection buffer pool, one section of described test agent box body is fixed with glue head by groove
Dropper, capillary pipette and stirring rod, described fluorescin culture medium and luminous detection buffer pool are transparent utensil.
Preferably, the lower section of described luminous detection buffer pool is provided with zone of heating, is provided with heating wire in zone of heating.
Preferably, the inside of described fluorescin culture medium is provided with standard ATP, and the side wall of fluorescin culture medium is carved
There is scale
Preferably, the inside of described test agent box body is fixed with miniature object weight sensing instrument, and fixation in miniature object weight sensing instrument sets
There is check weighing disk, the side of check weighing disk is fixed with display table, and the measurement range of miniature object weight sensing instrument is 0.01g to 50g.
Compared with prior art, beneficial effects of the present invention are as follows：
1st, staff can be allowed intuitively to see the result that microorganism has or not, save the time, simplified flow process；
2nd, pass through to obtain the net weight of microorganism in the heating of luminous detection buffer pool, can be obtained in original culture fluid by calculating
The content of microorganism.
Brief description
Fig. 1 is the overall structure diagram of kit for anti-interference quick detection of microbe quantity by bioluminescence method of the present invention；
Fig. 2 is that the fluorescin culture based structures in kit for anti-interference quick detection of microbe quantity by bioluminescence method of the present invention show
It is intended to；
Fig. 3 is the luminous detection buffer pool structure in kit for anti-interference quick detection of microbe quantity by bioluminescence method of the present invention
Schematic diagram.
In figure：1- test agent box body, 2- glue head dropper, 3- capillary pipette, 4- stirring rod, 5- miniature object weight sensing instrument, 6-
Check weighing disk, 7- shows table, 8- blue light photometer, 9- luminous detection buffer pool, 10- induction apparatuss, 11- high performance liquid chromatography
Instrument, 12- alarm lamp, 13- fluorescin culture medium, 14- standard ATP and other mixture of nutrients, 15- heating wire, 16- adds
Thermosphere, 17- scale.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation description is it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of not making creative work
Embodiment, broadly falls into the scope of protection of the invention.
Refer to Fig. 1-3, the present invention provides a kind of technical scheme：A kind of anti-interference quick inspection of microbe quantity by bioluminescence method
Test agent box, including test agent box body 1, fluorescin culture medium 13 and luminous detection buffer pool 9, described test agent box body 1
Inside is fixed with fluorescin culture medium 13, and the side of described fluorescin culture medium 13 is fixed with luminous detection buffer
Pond 9, the edge of described test agent box body 1 is fixed with blue light photometer 8 and high performance liquid chromatograph 11, described efficient liquid phase
Induction apparatuss 10 and alarm lamp 12 are provided with chromatograph 11, described alarm lamp 12 is provided with HONGGUANG bulb, is just arranged on luminous
The two ends of detection buffer pool 9, one section of described test agent box body 1 is fixed with glue head dropper 2, capillary pipette 3 by groove
With stirring rod 4, described fluorescin culture medium 13 and luminous detection buffer pool 9 are transparent utensil.
Operation principle：After microorganism is put into culture a period of time in fluorescin culture medium 9, with glue head dropper 2
Transfer part culture fluid, observes whether solution green glow afterwards under the irradiation of blue light photometer 8, thus proving to have or not to send out
Optical microbial, and after heating wire 15 evaporating water in zone of heating 16 on miniature object weight sensing instrument 5 measurement data thus
Calculate the data of microorganism in former culture medium.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, permissible
Understand and can carry out multiple changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (4)
1. a kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method, including test agent box body（1）, fluorescin training
Foster base（13）With luminous detection buffer pool（9）It is characterised in that described test agent box body（1）Inside is fixed with fluorescence egg
White culture medium（13）, described fluorescin culture medium（13）Side be fixed with luminous detection buffer pool（9）, described test
Agent box body（1）Edge be fixed with blue light photometer（8）And high performance liquid chromatograph（11）, described high performance liquid chromatograph
（11）On be provided with induction apparatuss（10）And alarm lamp（12）, described alarm lamp（12）It is provided with HONGGUANG bulb, be just arranged on and send out
Light detects buffer pool（9）Two ends, described test agent box body（1）One section glue head dropper is fixed with by groove（2）, hair
Thin suction pipe（3）And stirring rod（4）, described fluorescin culture medium（13）With luminous detection buffer pool（9）It is transparent utensil.
2. a kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method according to claim 1, its feature exists
In：Described luminous detection buffer pool（9）Lower section be provided with zone of heating（16）, zone of heating（16）Inside it is provided with heating wire（15）.
3. a kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method according to claim 1, its feature exists
In：Described fluorescin culture medium（13）Inside be provided with standard ATP, fluorescin culture medium（13）Side wall on be carved with quarter
Degree（17）.
4. a kind of kit for anti-interference quick detection of microbe quantity by bioluminescence method according to claim 1, its feature exists
In：Described test agent box body（1）Inside be fixed with miniature object weight sensing instrument（5）, miniature object weight sensing instrument（5）Interior fixation sets
There is check weighing disk（6）, check weighing disk（6）Side be fixed with display table（7）, miniature object weight sensing instrument（5）Measurement range be
0.01g to 50g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610974744.2A CN106383104A (en) | 2016-11-07 | 2016-11-07 | Anti-interference rapid detection kit for bioluminescence microbial population |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610974744.2A CN106383104A (en) | 2016-11-07 | 2016-11-07 | Anti-interference rapid detection kit for bioluminescence microbial population |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106383104A true CN106383104A (en) | 2017-02-08 |
Family
ID=57956915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610974744.2A Pending CN106383104A (en) | 2016-11-07 | 2016-11-07 | Anti-interference rapid detection kit for bioluminescence microbial population |
Country Status (1)
| Country | Link |
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| CN (1) | CN106383104A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442481A (en) * | 2016-11-21 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Bioluminescent microbe quantity anti-interference rapid detection kit |
| CN107831122A (en) * | 2017-10-27 | 2018-03-23 | 无锡艾科瑞思产品设计与研究有限公司 | It is a kind of to be used for the kit that chromium detects in water |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1455243A (en) * | 2002-04-29 | 2003-11-12 | 拜尔公司 | Method and apparatus for detecting biological active matter |
| CN1876829A (en) * | 2006-03-17 | 2006-12-13 | 广东省微生物研究所 | Kit for anti-interference quick detection of microbe quantity by bioluminescence method |
| CN1914495A (en) * | 2004-01-30 | 2007-02-14 | Qtl生物系统有限公司 | Systems, methods and reagents for the detection of biological and chemical agents using dynamic surface generation and imaging |
| CN101368910A (en) * | 2007-08-15 | 2009-02-18 | 中国科学院电子学研究所 | Optical reaction tank for triphosadenine bioluminescence detection |
| WO2009022329A1 (en) * | 2007-08-13 | 2009-02-19 | Check Light Ltd | Method for detecting microbes |
| CN102175661A (en) * | 2011-01-25 | 2011-09-07 | 宇星科技发展(深圳)有限公司 | Online analyzer of Escherichia coli |
| WO2014193999A2 (en) * | 2013-05-28 | 2014-12-04 | Caris Science, Inc. | Biomarker methods and compositions |
-
2016
- 2016-11-07 CN CN201610974744.2A patent/CN106383104A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1455243A (en) * | 2002-04-29 | 2003-11-12 | 拜尔公司 | Method and apparatus for detecting biological active matter |
| CN1914495A (en) * | 2004-01-30 | 2007-02-14 | Qtl生物系统有限公司 | Systems, methods and reagents for the detection of biological and chemical agents using dynamic surface generation and imaging |
| CN1876829A (en) * | 2006-03-17 | 2006-12-13 | 广东省微生物研究所 | Kit for anti-interference quick detection of microbe quantity by bioluminescence method |
| WO2009022329A1 (en) * | 2007-08-13 | 2009-02-19 | Check Light Ltd | Method for detecting microbes |
| CN101368910A (en) * | 2007-08-15 | 2009-02-18 | 中国科学院电子学研究所 | Optical reaction tank for triphosadenine bioluminescence detection |
| CN102175661A (en) * | 2011-01-25 | 2011-09-07 | 宇星科技发展(深圳)有限公司 | Online analyzer of Escherichia coli |
| WO2014193999A2 (en) * | 2013-05-28 | 2014-12-04 | Caris Science, Inc. | Biomarker methods and compositions |
Non-Patent Citations (5)
| Title |
|---|
| 付溥博等: "食品微生物检测试剂盒评价方法建立与应用", 《中国公共卫生》 * |
| 叶海仁等: "绿色荧光蛋白分子标记在环境微生物学研究中的应用", 《环境污染与防治》 * |
| 朱光富: "快速微生物检测试剂盒和快速检测仪", 《现代商件科技》 * |
| 田涛等: "绿色荧光蛋白作为分子标记物在微生物学中的应用", 《微生物学杂志》 * |
| 罗启仕等: "利用GFP 标记基因检测环境微生物", 《中国给水排水》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106442481A (en) * | 2016-11-21 | 2017-02-22 | 百奥森（江苏）食品安全科技有限公司 | Bioluminescent microbe quantity anti-interference rapid detection kit |
| CN107831122A (en) * | 2017-10-27 | 2018-03-23 | 无锡艾科瑞思产品设计与研究有限公司 | It is a kind of to be used for the kit that chromium detects in water |
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Application publication date: 20170208 |