CN106367333B - A kind of tubular type bioreactor CO2Device for thinning - Google Patents

A kind of tubular type bioreactor CO2Device for thinning Download PDF

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Publication number
CN106367333B
CN106367333B CN201610994190.2A CN201610994190A CN106367333B CN 106367333 B CN106367333 B CN 106367333B CN 201610994190 A CN201610994190 A CN 201610994190A CN 106367333 B CN106367333 B CN 106367333B
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China
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gas
liquid
disk
working cavity
valve
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CN201610994190.2A
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Chinese (zh)
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CN106367333A (en
Inventor
杨卫民
何立臣
关昌峰
张有忱
阎华�
丁玉梅
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北京化工大学
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation

Abstract

The invention discloses a kind of tubular type bioreactor CO2Device for thinning, include mainly filter device, Liquid valve, fluid flowmeter, gas valve, gas flowmeter, gas-liquid mixed threeway, axis flow path stream combined type labyrinth screw pump, microbubble fluid valve and microbubble liquid threeway, axis flow path stream combined type labyrinth screw pump includes pump intake, stator, rotor, fixed disk, refinement disk, spiral case and pump discharge.Rotor and stator form axis stream working cavity, and fixed disk and refinement disk form runoff working cavity.Axis flow path stream combined type labyrinth screw pump CO of the present invention2Device for thinning can be by CO2Gas is driven into micron order scale in tubular type bioreactor, evade rotor and requires high problem with stator installation accuracy, gas is refined by two-stage, fining air bubbles effect is good, microbubble is long in the pipeline residence time, it is unlikely to float to tube top portion quickly, gas-liquid mass transfer and more fully mixed, microalgae is to CO2Absorption it is more thorough, to preferably carrying out photosynthesis.

Description

A kind of tubular type bioreactor CO2Device for thinning

Technical field

The present invention relates to microdisk electrode engineering field, more particularly to a kind of tubular type bioreactor CO2Device for thinning.

Background technology

Microalgae is the photosynthetic microorganism being distributed widely in land and ocean, can efficiently use luminous energy, carbon dioxide, water With the bioactive substance of inorganic salts synthetic protein, fat, carbohydrate and high added value, thus it is in food, the energy And environmental area is gathered around and has broad application prospects.

Microalgae large scale and high density culture is the key link of microalgae biological industry, and this relies on high performance photo-biological Reactor, therefore the research of the bioreactor to cultivating microalgae is just always the Important Problems of microalgae living nature research.

Currently, microdisk electrode bioreactor mainly have it is open and two kinds closed.Closed photo bioreactor It is such as 1. pollution-free with lot of advantages, it can realize single, purebred culture;2. condition of culture is easy to control;3. culture density is high, Easily harvest;4. being suitable for the light autotrophy culture of all microalgaes;5. have the ratio between higher illuminating area and volume of culture, luminous energy and CO2Utilization rate is higher, therefore it has become developing direction from now on.

Closed photo bioreactor mainly has flat, column gas-lifting type, tubular type, stirred-tank fermenter, vertical at present Hanging bag, floating bag film.

Tubular type bioreactor because of large specific surface area, the efficiency of light energy utilization is high, gas-liquid mixed and mass transfer effect are good, flow velocity holds The advantages that easy to control and the industrialization culture for being widely used in microalgae.

With CO2Gas is that the bioreactor of carbon source must assure that CO2Gas is common by good dispersion and absorption Way is by CO2It is passed into bioreactor in the form of bubble, and this relies on CO2Device for thinning is realized.It is existing CO2The bubble scale that device for thinning types it out is grade, due to the difference of density, no matter the algae solution in pipe be in flowing or Stationary state, bubble are passed into the top for all floating on pipe after tubular type bioreactor substantially, this is unfavorable for microalgae to CO2It is good It is good to absorb, thus cannot also ensure that microalgae carries out sufficient photosynthesis.

The single-ended compound tooth-shaped labyrinth screw pump that the patent No. 200420007044.9 is announced, the patent No. The 201410304593.0 single-ended saw-tooth threads announced are from the Mazy spirals bodies such as the micro-nano air bubble apparatus of formula conveyor screw, rotor The gap that axis stream working cavity is formed by with stator is the key factor for influencing bubble size, if gap size is excessive, that Bubble size from pump out is big, and the thinning effect of bubble is poor;If gap size is too small, theoretically bubble size also may be used With very little, but the installation accuracy pumped in practice is extremely difficult to.

Invention content

The purpose of the invention is to overcome existing tubular type bioreactor CO2Device for thinning and existing labyrinth spiral shell The deficiency for revolving body, provides a kind of by CO2Bubble scale breaks the pipe for micron-sized Novel shaft flow path stream combined type labyrinth screw pump Formula bioreactor CO2Device for thinning makes CO2Bubble is scattered in very small state in tubular type optical-biological reaction pipeline, It can ensure microalgae and CO2Gas fully contacts, to carry out good photosynthesis.

In order to achieve the above object, the technical solution adopted in the present invention is as follows:

A kind of tubular type bioreactor CO2Device for thinning includes filter device, Liquid valve, fluid flowmeter, gas Valve, gas flowmeter, gas-liquid mixed threeway, axis flow path stream combined type labyrinth screw pump, microbubble fluid valve and microbubble liquid Threeway, axis flow path stream combined type labyrinth screw pump include pump intake, stator, rotor, fixed disk, refinement disk, spiral case and pump Outlet, fixed disk and refinement disk are the disc-shaped structure with prismatic blade or bent blades.

A kind of tubular type bioreactor CO2Device for thinning is applied to a kind of tubular type bioreactor, including water tank, stream Journey pump, algae solution valve, light reaction pipeline.

When work, filter device is placed in water tank, the threeway of microbubble liquid and light reaction pipeline by screw thread or flange into Row connection;Motor drives rotor and refinement disk high speed rotation, by the liquid in water tank through filter device, Liquid valve, liquid flow Gauge, gas-liquid mixed threeway, pump intake are drawn into axis flow path stream combined type labyrinth screw pump, while also by CO2- air mixes Gas is drawn into axis flow path stream combined type labyrinth screw pump through gas valve, gas flowmeter, gas-liquid mixed threeway, pump intake, The flow of liquids and gases is controlled by adjusting the aperture of Liquid valve and gas valve respectively, the flow of liquids and gases Value is read by fluid flowmeter and gas flow meter respectively;Rotor and stator form axis stream working cavity, fixed disk and refinement disk Runoff working cavity is formed, the gap of runoff working cavity is less than the gap of axis stream working cavity;Gas-liquid two-phase fluid is in axis stream It circles and is axially moved in working cavity, then go through and circle in runoff working cavity and radially transport It is dynamic;CO2- air gas mixture is first rubbed and is sheared at high speed in axis stream working cavity, then into runoff working cavity It is further rubbed and is sheared at high speed, ultimately form the doping of a large amount of micro-bubble in a liquid through microbubble fluid valve and micro- Bubble liquid threeway is flowed into light reaction pipeline.

Microalgae and culture solution in bioreactor circulate under the action of process pump along light reaction pipeline, real Existing coming into full contact between microalgae and micro-bubble ensures that microalgae carries out complete photosynthesis.

The hole of filter device is less than the diameter of microalgae cell, can flow into axis flow path stream combined type labyrinth in this way Liquid in helicoidal pump does not contain microalgae cell, so as to avoid axis flow path stream combined type labyrinth screw pump to the wound of microalgae cell Evil.

Fixed disk and refinement disk are prismatic blade or bent blades, and fixed disk and refinement disk may be prismatic blade or bent blades two Two are arbitrarily composed, and gas and liquid mixture tentatively refines between stator and rotor, and the mixed liquor tentatively refined is through fixed disk and carefully Change the further bubble disintegration back edge of disk to throw away, the structure of double ranks makes the fining air bubbles in mixed liquor complete.

The beneficial effects of the invention are as follows axis flow path stream combined type labyrinth screw pump CO2Device for thinning can be by CO2Gas with Micron order scale is driven into tubular type bioreactor, has been evaded rotor and has been required high problem, gas with stator installation accuracy It is refined by two-stage, fining air bubbles effect is good, and microbubble is long in the pipeline residence time, is unlikely to float to tube top portion, gas quickly Liquid two-phase mass transfer and more fully mixed, microalgae is to CO2Absorption it is more thorough, to preferably carrying out photosynthesis.

Description of the drawings

Fig. 1 is a kind of tubular type bioreactor CO of the present invention2Device for thinning.

Fig. 2 is a kind of tubular type bioreactor CO of the present invention2The volute structure of device for thinning.

Fig. 3 is a kind of tubular type bioreactor CO of the present invention2The prismatic blade refinement disk or fixed disk of device for thinning.

Fig. 4 is a kind of tubular type bioreactor CO of the present invention2The bent blades refinement disk or fixed disk of device for thinning.

Fig. 5 is a kind of tubular type bioreactor CO of the present invention2Device for thinning is applied to a kind of tubular type bioreactor.

In figure, 1-filter device, 2-Liquid valves, 3-fluid flowmeters, 4-gas valves, 5-gas flows Meter, 6-gas-liquid mixed threeways, 7-axis flow path stream combined type labyrinth screw pumps, wherein 7-1-pump intake, 7-2-stator, 7- 3-rotors, 7-4-fixation disk, 7-5-refinement disk, 7-6-spiral case, 7-7-pump discharge, 8-microbubble fluid valves, 9-micro- gas Steep liquid threeway, 10-water tanks, 11-process pumps, 12-algae solution valves, 13-light reaction pipelines.

Specific implementation mode

The present invention is described in detail below with reference to the accompanying drawings.

As shown in Figure 1, a kind of tubular type bioreactor CO of the present invention2Device for thinning includes filter device 1, Liquid valve 2, fluid flowmeter 3, gas valve 4, gas flowmeter 5, gas-liquid mixed threeway 6, axis flow path stream combined type labyrinth screw pump 7, Microbubble fluid valve 8 and microbubble liquid threeway 9, axis flow path stream combined type labyrinth screw pump 7 include pump intake 7-1, stator 7-2, rotor 7-3, fixed disk 7-4, refinement disk 7-5, spiral case 7-6 and pump discharge 7-7.

As shown in figure 5, a kind of tubular type bioreactor CO of the present invention2It is anti-that device for thinning is applied to a kind of tubular type photo-biological Answer device, including water tank 10, process pump 11, algae solution valve 12, light reaction pipeline 13.

When work, filter device 1 is placed in water tank 10, microbubble liquid threeway 9 and light reaction pipeline 13 by screw thread or Flange is attached;Motor drive rotor 7-3 and refinement disk 7-5 high speed rotations, by the liquid in water tank 10 through filter device 1, Liquid valve 2, fluid flowmeter 3, gas-liquid mixed threeway 6, pump intake 7-1 are drawn into axis flow path stream combined type labyrinth screw pump 7, while also by CO2- air gas mixture is inhaled through gas valve 4, gas flowmeter 5, gas-liquid mixed threeway 6, pump intake 7-1 Enter to axis flow path stream combined type labyrinth screw pump 7, the flow of liquids and gases is respectively by adjusting Liquid valve 2 and gas trap The aperture of door 4 is controlled, and the flow value of liquids and gases is read by 3 and gas flow meter 5 of fluid flowmeter respectively;Rotor 7-3 and stator 7-2 forms axis stream working cavity, and fixed disk 7-4 and refinement disk 7-5 form runoff working cavity, runoff working chamber The gap of body is less than the gap of axis stream working cavity, and fixed disk 7-4 and refinement disk 7-5 are located in spiral case 7-6 (as shown in Figure 2); Gas-liquid two-phase fluid is circled and is axially moved in axis stream working cavity, is then gone through in runoff working cavity and is made Circular motion and radial motion;CO2- air gas mixture first is rubbed and is sheared at high speed in axis stream working cavity, then into Enter to runoff working cavity and further rubbed and sheared at high speed, it is micro- in a liquid to ultimately form a large amount of micro-bubble doping Bubble is flowed into light reaction pipeline 13 through fluid valve 8 and microbubble liquid threeway 9 again after the pump discharge 7-7 of spiral case 7-6.

Microalgae and culture solution in bioreactor are under the action of process pump 11 along 13 recycle stream of light reaction pipeline It is dynamic, it realizes coming into full contact between microalgae and micro-bubble, ensures that microalgae carries out complete photosynthesis.

The hole of filter device 1 is less than the diameter of microalgae cell, can flow into axis flow path stream combined type fan in this way Liquid in palace helicoidal pump 7 does not contain microalgae cell, so as to avoid axis flow path stream combined type labyrinth screw pump 7 to microalgae cell Injury.

Fixed disk 7-4 and refinement disk 7-5 can be carried out two-by-two by prismatic blade (as shown in Figure 3) or bent blades (shown in Fig. 4) Arbitrary combination.

The preferred embodiment of the present invention is rotor 7-3 and stator 7-2 is formed by the gap of axis stream working cavity and is The gap that 0.2mm, fixed disk 7-4 and refinement disk 7-5 are formed by runoff working cavity is 0.1mm, and two gaps too small give adds Work and installation bring difficulty, the bubble diameter of the too big refinement in two gaps to be extremely difficult to micron order.

It will be clear that the explanation of embodiment of the present invention is intended to explain it above by reference to attached drawing, without It is interpreted as limitation of the present invention.Limited range of the present invention is referring to claim.Core situation of the present invention is not being departed from Under, those skilled in the art do not spend creative work, any modification and improvement to the present invention, to component selected by the present invention Equivalence replacement be within the scope of the present invention.

Claims (4)

1. a kind of tubular type bioreactor CO2Device for thinning, it is characterised in that:Including filter device, Liquid valve, liquid flow Gauge, gas valve, gas flowmeter, gas-liquid mixed threeway, axis flow path stream combined type labyrinth screw pump, microbubble fluid valve and Microbubble liquid threeway, axis flow path stream combined type labyrinth screw pump include pump intake, stator, rotor, fixed disk, refinement disk, Spiral case and pump discharge, fixed disk and refinement disk are the disc-shaped structure with prismatic blade or bent blades, and filter device is placed in water tank Interior, the threeway of microbubble liquid is attached with light reaction pipeline by screw thread or flange;Motor drives rotor and refinement disk high speed Rotation, axis is drawn by the liquid in water tank through filter device, Liquid valve, fluid flowmeter, gas-liquid mixed threeway, pump intake Flow path stream combined type labyrinth screw pump, while also by CO2- air gas mixture is mixed through gas valve, gas flowmeter, gas-liquid Conjunction threeway, pump intake are drawn into axis flow path stream combined type labyrinth screw pump, and the flow of liquids and gases passes through respectively adjusts liquid The aperture of valve and gas valve is controlled, and the flow value of liquids and gases is read by fluid flowmeter and gas flow meter respectively Go out;Rotor and stator form axis stream working cavity, fix disk and refine disk formation runoff working cavity, between runoff working cavity Gap is less than the gap of axis stream working cavity;Gas-liquid two-phase fluid is circled and is axially moved in axis stream working cavity, It then goes through and is circled in runoff working cavity and radial motion;CO2- air gas mixture is first in axis stream working chamber Body is rubbed and is sheared at high speed, is further rubbed and is sheared at high speed then into runoff working cavity, ultimately formed A large amount of micro-bubble doping is flowed into light reaction pipeline through microbubble fluid valve and microbubble liquid threeway in a liquid.
2. a kind of tubular type bioreactor CO according to claim 12Device for thinning, it is characterised in that:Filter device Hole is less than the diameter of microalgae cell.
3. a kind of tubular type bioreactor CO according to claim 12Device for thinning, it is characterised in that:Fixed disk and thin It is that prismatic blade or bent blades are arbitrarily composed two-by-two to change disk.
4. a kind of tubular type bioreactor CO according to claim 12Device for thinning, it is characterised in that:Rotor and stator The gap for being formed by axis stream working cavity is 0.2mm, and the gap that fixation disk and refinement disk are formed by runoff working cavity is 0.1mm。
CN201610994190.2A 2016-11-11 2016-11-11 A kind of tubular type bioreactor CO2Device for thinning CN106367333B (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2697350Y (en) * 2004-03-17 2005-05-04 北京化工大学 Single end composite tooth type labyrinth screw pump
CN2877837Y (en) * 2006-06-23 2007-03-14 北京环尔康科技开发有限公司 Thinning apparatus for liquid
CN201135388Y (en) * 2007-12-13 2008-10-22 杨仁勇 Micro- bubble generation device
CN102985172A (en) * 2010-07-15 2013-03-20 韩国机械研究院 Micro bubble generation device based on rotating unit
CN103861488A (en) * 2014-03-19 2014-06-18 中国船舶重工集团公司第七○二研究所 Micro-bubble generating device
CN105240269A (en) * 2014-06-26 2016-01-13 水利部科技推广中心 Double-end U-type thread divided spiral micro-nano bubble device
CN206157157U (en) * 2016-11-11 2017-05-10 北京化工大学 Tubular photobioreactor CO2 refines device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2697350Y (en) * 2004-03-17 2005-05-04 北京化工大学 Single end composite tooth type labyrinth screw pump
CN2877837Y (en) * 2006-06-23 2007-03-14 北京环尔康科技开发有限公司 Thinning apparatus for liquid
CN201135388Y (en) * 2007-12-13 2008-10-22 杨仁勇 Micro- bubble generation device
CN102985172A (en) * 2010-07-15 2013-03-20 韩国机械研究院 Micro bubble generation device based on rotating unit
CN103861488A (en) * 2014-03-19 2014-06-18 中国船舶重工集团公司第七○二研究所 Micro-bubble generating device
CN105240269A (en) * 2014-06-26 2016-01-13 水利部科技推广中心 Double-end U-type thread divided spiral micro-nano bubble device
CN206157157U (en) * 2016-11-11 2017-05-10 北京化工大学 Tubular photobioreactor CO2 refines device

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
串接组合式迷宫螺旋泵的气液两相流数值模拟及试验研究;张塞等;《工业安全与环保》;20160331;第42卷(第3期);14-17 *
迷宫螺旋泵气液两相流场的数值模拟及试验;张有忱等;《排灌机械工程学报》;20101130;第28卷(第6期);492-496 *

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