CN106367333A - Tubular photo-biological reactor CO2 refining device - Google Patents

Tubular photo-biological reactor CO2 refining device Download PDF

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Publication number
CN106367333A
CN106367333A CN201610994190.2A CN201610994190A CN106367333A CN 106367333 A CN106367333 A CN 106367333A CN 201610994190 A CN201610994190 A CN 201610994190A CN 106367333 A CN106367333 A CN 106367333A
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China
Prior art keywords
gas
liquid
working cavity
valve
refining
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CN201610994190.2A
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Chinese (zh)
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CN106367333B (en
Inventor
杨卫民
何立臣
关昌峰
张有忱
阎华�
丁玉梅
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北京化工大学
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation

Abstract

The invention discloses a tubular photo-biological reactor CO2 refining device. The tubular photo-biological reactor CO2 refining device mainly comprises a filter device, a liquid valve, a liquid flowmeter, a gas valve, a gas flowmeter, a gas-liquid mixed tee, an axial flow and radial flow composite labyrinth screw pump, a microbubble liquid valve and a microbubble liquid tee, wherein the axial flow and radial flow composite labyrinth screw pump comprises a pump inlet, a stator, a rotor, a fixed disc, a refining disc, a volute and a pump outlet. The rotor and the stator form an axial flow working cavity, and the fixed disc and the refining disc form a radial flow working cavity. According to the axial flow and radial flow composite labyrinth screw pump CO2 refining device disclosed by the invention, CO2 gas can be pumped into a tubular photo-biological reactor in a micron-order scale, the problems that the requirment on installation accuracy of the rotor and the stator is high is avoided, the bubble refining effect is good through two-stage refining of gas, the residence time of microbubbles in pipelines is long, the microbubbles are unlikely to float to the top of a pipe quickly, gas-liquid two-phase mass transfer and mixing are more sufficient, and the absorption of microalgae for CO2 is more thorough, so that photosynthesis is carried out preferably.

Description

A kind of tubular type bioreactor co2Device for thinning

Technical field

The present invention relates to microdisk electrode engineering field, particularly to a kind of tubular type bioreactor co2Device for thinning.

Background technology

Microalgae is the photosynthetic microorganism being distributed widely in land and ocean, and it can effectively utilizes luminous energy, carbon dioxide, water With the bioactive substance of inorganic salt synthetic protein, fat, carbohydrate and high added value, thus it is in food, the energy And environmental area gathers around and has broad application prospects.

The culture of microalgae large scale and high density is the key link of microalgae biological industry, and this relies on high performance photo bio Reactor, is therefore just always the Important Problems of microalgae biosphere research to the research of the bioreactor of culture microalgae.

At present, microdisk electrode bioreactor mainly have open and closed two kinds.Closed photo bioreactor There is lot of advantages, such as 1. pollution-free, enable single, purebred culture;2. condition of culture is easily controlled;3. culture density high, Easily harvest;4. it is suitable for the light autotrophy culture of all microalgae;5. have a ratio of higher illuminating area and volume of culture, luminous energy and co2Utilization rate is higher, and therefore it has become developing direction from now on.

At present closed photo bioreactor mainly has flat, column gas-lifting type, tubular type, stirred-tank fermenter, vertical Pig, floating bag film.

Tubular type bioreactor is because specific surface area is big, the efficiency of light energy utilization is high, gas-liquid mixed and mass transfer effect is good, flow velocity holds The advantages of easy to control and be widely used in microalgae industrialization culture.

With co2Gas is that the bioreactor of carbon source must assure that co2Gas is by good dispersion and absorption, common Way is by co2It is passed in bioreactor in the form of bubble, and this relies on co2Device for thinning is realizing.Existing co2The bubble scale that device for thinning types it out is grade, due to the difference of density, no matter the algae solution in pipe be in flowing or Resting state, bubble all floats over the top of pipe substantially after being passed into tubular type bioreactor, this is unfavorable for microalgae to co2Good Good absorption, thus also cannot ensure that microalgae carries out sufficient photosynthesis.

The single-ended compound tooth-shaped labyrinth screw pump that the patent No. 200420007044.9 is announced, the patent No. 201410304593.0 In the Mazy spiral bodies such as the micro-nano air bubble apparatus of formula spirillum, rotor and stator are formed the single-ended saw-tooth thread announced The gap of axle stream working cavity is the key factor of impact bubble size, if gap size is excessive, then from pump out Bubble size is big, and the thinning effect of bubble is poor;If gap size is too small, in theory bubble size can also very little, but actual The installation accuracy of middle pump is extremely difficult to.

Content of the invention

The invention aims to overcoming existing tubular type bioreactor co2Device for thinning and existing labyrinth spiral shell The deficiency of rotation body, provide a kind of by co2Bubble scale breaks the pipe for micron-sized Novel shaft flow path stream combined type labyrinth screw pump Formula bioreactor co2Device for thinning, makes co2Bubble is scattered in tubular type optical-biological reaction pipeline with very small state, Ensure that microalgae and co2Gas fully contacts, thus carrying out good photosynthesis.

In order to achieve the above object, the technical solution adopted in the present invention is as follows:

A kind of tubular type bioreactor co2Device for thinning includes defecator, Liquid valve, fluid flowmeter, gas Valve, gas flowmeter, gas-liquid mixed threeway, axle flow path stream combined type labyrinth screw pump, microbubble fluid valve and microbubble liquid Threeway, its axis flow path stream combined type labyrinth screw pump includes pump intake, stator, rotor, fixed disk, refinement disk, spiral case and pump Outlet.

A kind of tubular type bioreactor co2Device for thinning is applied to a kind of tubular type bioreactor, including water tank, stream Journey pump, algae solution valve, photoreaction pipeline.

During work, defecator is placed in water tank, and microbubble liquid threeway and photoreaction pipeline are entered by screw thread or flange Row connects;Motor rotor driven and refinement disk high-speed rotation, by the liquid in water tank through defecator, Liquid valve, liquid flow Gauge, gas-liquid mixed threeway, pump intake are drawn into axle flow path stream combined type labyrinth screw pump, simultaneously also by co2Air mixes Gas is drawn into axle flow path stream combined type labyrinth screw pump through gas valve, gas flowmeter, gas-liquid mixed threeway, pump intake, The flow of liquids and gases is controlled by the aperture adjusting Liquid valve and gas valve respectively, the flow of liquids and gases Value is read by fluid flowmeter and gas flowmeter respectively;Rotor and stator form axle stream working cavity, fixed disk and refinement disk Form runoff working cavity, the gap of runoff working cavity is less than the gap of axle stream working cavity;Gas-liquid two-phase fluid is in axle stream Circle in working cavity and axially-movable, then go through and circle in runoff working cavity and radially transport Dynamic;co2Air gas mixture is first rubbed at high speed in axle stream working cavity and is sheared, then into runoff working cavity Rubbed at high speed further and sheared, ultimately formed the doping of substantial amounts of micro-bubble in a liquid through microbubble fluid valve and micro- Bubble liquid threeway is flowed into photoreaction pipeline.

Microalgae in bioreactor and culture fluid circulate along photoreaction pipeline in the presence of process pump, real Existing being fully contacted between microalgae and micro-bubble is it is ensured that microalgae carries out completely photosynthesis.

The hole of defecator is less than the diameter of microalgae cell, so so that being flowed into axle flow path stream combined type labyrinth Liquid in screw pump does not contain microalgae cell, thus avoiding the wound to microalgae cell for the axle flow path stream combined type labyrinth screw pump Evil.

Fixed disk and refinement disk are prismatic blade or bent blades, and fixed disk and refinement disk can also be prismatic blade or bent blades two Two combination in any form, and gas and liquid mixture tentatively refines between stator and rotor, and the mixed liquor of preliminary refinement is through fixed disk and thin Change disk further bubble disintegration back edge throws away, and the structure of double ranks makes the fining air bubbles in mixed liquor complete.

The invention has the beneficial effects as follows this axle flow path stream combined type labyrinth screw pump co2Device for thinning can be by co2Gas with Micron order yardstick is driven in tubular type bioreactor, has evaded the problem that rotor is had high demands, gas with stator installation accuracy Through two-stage refinement, fining air bubbles effect is good, and microbubble is long in the pipeline time of staying, is unlikely to float to pipe top, gas quickly The biphase mass transfer of liquid and mixing are more abundant, and microalgae is to co2Absorption more thorough, thus preferably carrying out photosynthesis.

Brief description

Fig. 1 is a kind of present invention tubular type bioreactor co2Device for thinning.

Fig. 2 is a kind of present invention tubular type bioreactor co2The worm frame of device for thinning.

Fig. 3 is a kind of present invention tubular type bioreactor co2The prismatic blade refinement disk of device for thinning or fixed disk.

Fig. 4 is a kind of present invention tubular type bioreactor co2The bent blades refinement disk of device for thinning or fixed disk.

Fig. 5 is a kind of present invention tubular type bioreactor co2Device for thinning is applied to a kind of tubular type bioreactor.

In figure, 1 defecator, 2 Liquid valves, 3 fluid flowmeters, 4 gas valves, 5 gas flowmeters, 6 gas-liquid mixed threeways, 7 axle flow path stream combined type labyrinth screw pumps, wherein 7-1 pump intake, 7-2 stator, 7-3 turns Son, 7-4 fixed disk, 7-5 refinement disk, 7-6 spiral case, 7-7 pump discharge, 8 microbubble fluid valves, 9 microbubble liquid Threeway, 10 water tanks, 11 process pumps, 12 algae solution valves, 13 photoreaction pipelines.

Specific embodiment

Below with reference to the accompanying drawings the present invention is described in detail.

As shown in figure 1, a kind of present invention tubular type bioreactor co2Device for thinning includes defecator 1, Liquid valve 2nd, fluid flowmeter 3, gas valve 4, gas flowmeter 5, gas-liquid mixed threeway 6, axle flow path stream combined type labyrinth screw pump 7, Microbubble fluid valve 8 and microbubble liquid threeway 9, its axis flow path stream combined type labyrinth screw pump 7 includes pump intake 7-1, stator 7-2, rotor 7-3, fixed disk 7-4, refinement disk 7-5, spiral case 7-6 and pump discharge 7-7.

As shown in figure 5, a kind of present invention tubular type bioreactor co2It is anti-that device for thinning is applied to a kind of tubular type photo bio Answer device, including water tank 10, process pump 11, algae solution valve 12, photoreaction pipeline 13.

During work, defecator 1 is placed in water tank 10, microbubble liquid threeway 9 and photoreaction pipeline 13 pass through screw thread or Flange is attached;Motor rotor driven 7-3 and refinement disk 7-5 high-speed rotation, by the liquid in water tank 10 through defecator 1, Liquid valve 2, fluid flowmeter 3, gas-liquid mixed threeway 6, pump intake 7-1 are drawn into axle flow path stream combined type labyrinth screw pump 7, Simultaneously also by co2Air gas mixture sucks through gas valve 4, gas flowmeter 5, gas-liquid mixed threeway 6, pump intake 7-1 To axle flow path stream combined type labyrinth screw pump 7, the flow of liquids and gases is respectively by adjusting Liquid valve 2 and gas valve 4 Aperture be controlled, the flow value of liquids and gases is read by fluid flowmeter 3 and gas flowmeter 5 respectively;Rotor 7-3 and Stator 7-2 forms axle stream working cavity, and fixed disk 7-4 and refinement disk 7-5 forms runoff working cavity, between runoff working cavity Gap is less than the gap of axle stream working cavity, and fixed disk 7-4 and refinement disk 7-5 is located in spiral case 7-6 (as shown in Figure 2);Gas-liquid two Phase fluid circles and axially-movable in axle stream working cavity, then goes through and makees circumference fortune in runoff working cavity Move and radial motion;co2Air gas mixture is first rubbed at high speed in axle stream working cavity and is sheared, then into footpath Stream working cavity is rubbed at high speed further and is sheared, and ultimately forms substantial amounts of micro-bubble doping microbubble warp in a liquid It is flowed into photoreaction pipeline 13 through fluid valve 8 and microbubble liquid threeway 9 again after the pump discharge 7-7 of spiral case 7-6.

Microalgae in bioreactor and culture fluid are in the presence of process pump 11 along photoreaction pipeline 13 recycle stream Dynamic, realize being fully contacted between microalgae and micro-bubble it is ensured that microalgae carries out completely photosynthesis.

The hole of defecator 1 is less than the diameter of microalgae cell, so so that being flowed into axle flow path stream combined type fan Liquid in palace screw pump 7 does not contain microalgae cell, thus avoiding axle flow path stream combined type labyrinth screw pump 7 to microalgae cell Injury.

Fixed disk 7-4 and refinement disk 7-5 can be carried out two-by-two by prismatic blade (as shown in Figure 3) or bent blades (as shown in Figure 4) Combination in any.

The gap of the axle stream working cavity that the preferred embodiments of the present invention are formed by rotor 7-3 and stator 7-2 is The gap of the runoff working cavity that 0.2mm, fixed disk 7-4 and refinement disk 7-5 are formed is 0.1mm, two gaps too little to plus Work and installation bring difficulty, and the bubble diameter of the too big refinement in two gaps is extremely difficult to micron order.

It will be clear that being intended to it is explained to the explanation of embodiment of the present invention above by reference to accompanying drawing, and not It is interpreted as limitation of the present invention.Limited range of the present invention is referring to claim.Without departing from core situation of the present invention Under, those skilled in the art do not spend creative work, any modification to the present invention and improvement, to part selected by the present invention Equivalence replacement be within the scope of the present invention.

Claims (4)

1. a kind of tubular type bioreactor co2 device for thinning it is characterised in that: include defecator, Liquid valve, liquid flow Gauge, gas valve, gas flowmeter, gas-liquid mixed threeway, axle flow path stream combined type labyrinth screw pump, microbubble fluid valve and Microbubble liquid threeway, its axis flow path stream combined type labyrinth screw pump include pump intake, stator, rotor, fixed disk, refinement disk, Spiral case and pump discharge, defecator is placed in water tank, and microbubble liquid threeway is carried out by screw thread or flange with photoreaction pipeline Connect;Motor rotor driven and refinement disk high-speed rotation, by the liquid in water tank through defecator, Liquid valve, fluid flow Meter, gas-liquid mixed threeway, pump intake are drawn into axle flow path stream combined type labyrinth screw pump, simultaneously also by co2Air Mixture Body is drawn into axle flow path stream combined type labyrinth screw pump, liquid through gas valve, gas flowmeter, gas-liquid mixed threeway, pump intake The flow of body and gas is controlled by the aperture adjusting Liquid valve and gas valve respectively, the flow value of liquids and gases Read by fluid flowmeter and gas flowmeter respectively;Rotor and stator form axle stream working cavity, fixed disk and refinement dish type Become runoff working cavity, the gap of runoff working cavity is less than the gap of axle stream working cavity;Gas-liquid two-phase fluid is in axle stream work Make to circle in cavity and axially-movable, then go through in runoff working cavity and circle and radial motion; co2Air gas mixture is first rubbed at high speed in axle stream working cavity and is sheared, and enters then into runoff working cavity One step is rubbed at high speed and is sheared, and ultimately forms substantial amounts of micro-bubble and adulterates in a liquid through microbubble fluid valve and micro- gas Bubble liquid threeway is flowed into photoreaction pipeline.
2. a kind of tubular type bioreactor co2 device for thinning according to claim 1 it is characterised in that: defecator Hole be less than microalgae cell diameter.
3. a kind of tubular type bioreactor co2 device for thinning according to claim 1 it is characterised in that: fixed disk and Refinement disk is prismatic blade or bent blades, and fixed disk and refinement disk are that combination in any forms two-by-two for prismatic blade or bent blades.
4. a kind of tubular type bioreactor co2 device for thinning according to claim 1 it is characterised in that: rotor and fixed The gap of axle stream working cavity that son is formed is 0.2mm, the gap of the runoff working cavity that fixed disk and refinement disk are formed For 0.1mm.
CN201610994190.2A 2016-11-11 2016-11-11 A kind of tubular type bioreactor CO2Device for thinning CN106367333B (en)

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Publication number Priority date Publication date Assignee Title
CN2697350Y (en) * 2004-03-17 2005-05-04 北京化工大学 Single end composite tooth type labyrinth screw pump
CN2877837Y (en) * 2006-06-23 2007-03-14 北京环尔康科技开发有限公司 Thinning apparatus for liquid
CN201135388Y (en) * 2007-12-13 2008-10-22 杨仁勇 Micro- bubble generation device
CN102985172A (en) * 2010-07-15 2013-03-20 韩国机械研究院 Micro bubble generation device based on rotating unit
CN103861488A (en) * 2014-03-19 2014-06-18 中国船舶重工集团公司第七○二研究所 Micro-bubble generating device
CN105240269A (en) * 2014-06-26 2016-01-13 水利部科技推广中心 Double-end U-type thread divided spiral micro-nano bubble device
CN206157157U (en) * 2016-11-11 2017-05-10 北京化工大学 Tubular photobioreactor CO2 refines device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2697350Y (en) * 2004-03-17 2005-05-04 北京化工大学 Single end composite tooth type labyrinth screw pump
CN2877837Y (en) * 2006-06-23 2007-03-14 北京环尔康科技开发有限公司 Thinning apparatus for liquid
CN201135388Y (en) * 2007-12-13 2008-10-22 杨仁勇 Micro- bubble generation device
CN102985172A (en) * 2010-07-15 2013-03-20 韩国机械研究院 Micro bubble generation device based on rotating unit
CN103861488A (en) * 2014-03-19 2014-06-18 中国船舶重工集团公司第七○二研究所 Micro-bubble generating device
CN105240269A (en) * 2014-06-26 2016-01-13 水利部科技推广中心 Double-end U-type thread divided spiral micro-nano bubble device
CN206157157U (en) * 2016-11-11 2017-05-10 北京化工大学 Tubular photobioreactor CO2 refines device

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Title
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