CN106365279A - Microbiological product capable of flocculation and coagulation enhancement and preparation method, use method and application thereof - Google Patents

Microbiological product capable of flocculation and coagulation enhancement and preparation method, use method and application thereof Download PDF

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Publication number
CN106365279A
CN106365279A CN201610897656.7A CN201610897656A CN106365279A CN 106365279 A CN106365279 A CN 106365279A CN 201610897656 A CN201610897656 A CN 201610897656A CN 106365279 A CN106365279 A CN 106365279A
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microorganism
wadding
salt
solidifying
solidifying product
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谭淞文
陈训财
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/52Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C02F1/5263Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using natural chemical compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/103Arsenic compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • C02F2101/32Hydrocarbons, e.g. oil
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • C02F2101/32Hydrocarbons, e.g. oil
    • C02F2101/327Polyaromatic Hydrocarbons [PAH's]
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • C02F2101/34Organic compounds containing oxygen
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • C02F2101/36Organic compounds containing halogen
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/08Seawater, e.g. for desalination

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a microbiological product capable of flocculation and coagulation enhancement, a preparation method, a use method and application thereof. The microbiological product capable of flocculation and coagulation enhancement is a biomacromolecule which is produced by marine microorganisms capable of flocculation and coagulation enhancement, has the effects of flocculation and coagulation enhancement and exists in the form of agent or chemical powder; the microbiological product capable of flocculation and coagulation enhancement has the advantages of being resistant to extreme environment, capable of being monitored in real time and diversified in product forms, the performance and application range of the microbiological product capable of flocculation and coagulation enhancement are improved. The preparation method, use method and application of the microbiological product capable of flocculation and coagulation enhancement achieve a low-cost sustainable production with high efficiency and high speed, enable the products to be operated conveniently and fast, and achieve the universality of the products.

Description

A kind of microorganism wadding helps solidifying product and preparation method thereof, using method and application
Technical field
The present invention relates to water-treatment technology field is and in particular to a kind of microorganism wadding helps solidifying product and preparation method thereof, makes Use methods and applications.
Background technology
It is that a class can act on preparation or the powder-product precipitating other materials, its quilt by adsorption bridging that wadding helps solidifying product It is widely used in the every field of water process, such as waste water control, chemical industry precipitates, and drinks water pretreatment etc..Wherein microorganism wadding helps Solidifying product has a preferable wadding effect of flocculant, the wide scope of application, and can be biodegradable, and safety non-toxic does harm to.Existing micro- Though biological wadding flocculation aid can reach preferable water treatment efficiency, also environmental protection, yet suffer from strain and obtain difficult, training Foster condition is harsh, volume production is difficult or the scope of application limited by the problems such as.
Record a kind of microorganism wadding processing organophosphors in waste water in prior art and helped solidifying product, it has been to originate from ancient tomb The biomacromolecule of room soil bacteria, after this fermentable is produced 3 ~ 4 days, is obtained with isopropyl alcohol extraction.This micro- life Thing wadding helps solidifying product flocculation activity preferably, can be used for partly dirty, the instant process of waste water, but because this strain is to be grown in non-salt Antibacterial under soil environment, institute's producing microbial wadding helps solidifying product can not be widely used in processing all kinds of salt-containing organic wastewaters;And This microorganism is wadded a quilt with cotton and help the production of solidifying product, separates and the technical requirements of preparation are also more harsh, and the time needed for fermenting and producing is relatively Long, it is difficult volume production;Additionally use organic solvent to be extracted, increased cost, cause secondary pollution.
Record a kind of complex microorganism wadding processing not calcium ions strong basicity waste water in prior art and help solidifying product, it It is to originate from the biomacromolecule buying the arthrobacterium (numbering atcc31012) in atcc company of the U.S., by sending out to this microorganism After ferment produces 60 ~ 80 hours, centrifugation is obtained.Because this microorganism wadding helps solidifying product can be combined with calcium chloride flocculant, fit For in relatively broad dirt, wastewater treatment, but because this strain is also the antibacterial being grown under non-salt soil environment, then produced Microorganism wadding helps solidifying product not high for activity when processing the compound wastewater containing inorganic salt;And produce this microorganism wadding and help solidifying product Product required time still longer, be difficult volume production;And this strain is purchased from elsewhere, increased the cost of industrialization production.
Therefore, seek new microorganism wadding and help solidifying product, to improve the scope of application and the life that microorganism wadding helps solidifying product Produce efficiency, reduce production difficulty and the production cost that microorganism wadding helps solidifying product, be the problem being currently needed for solving.
Content of the invention
For defect of the prior art, present invention aim at providing a kind of microorganism wadding to help solidifying product and its preparation side Method, using method and application, to improve performance, the scope of application and the production efficiency that microorganism wadding helps solidifying product, reduce microorganism Wadding helps production difficulty and the production cost of solidifying product.
For achieving the above object, the technical scheme that the present invention provides is:
In a first aspect, the invention provides a kind of microorganism wadding help solidifying product, be ocean wadding help solidifying micro-organisms, there is wadding Coagulate and Coagulation aiding, the biomacromolecule presented in medicament or medicated powder, and have many in three-dimensional fluorescence scanning optical spectrum Individual feature locations (λ ex/ λ em), comprise 230/290 nanometer, 260/445 nanometer, 275/325 nanometer, in 355/430 nanometer one Individual or several;Described biomacromolecule is humic acid class, fulvic acid class, phenols aminoacid, glycoprotein, mucopolysaccharide, protein, fibre One or more of dimension element, DNA (deoxyribonucleic acid).
Due to the presence of described feature locations, with fluorescent scanning instrument, monitor in real time can be carried out to coagulation water body, realize Digitized, remote-control, intelligent water chemical treatment;Because described biomacromolecule has flocculation and Coagulation aiding, can be used alone, join With, assist be used in all kinds of water treatment technologies;And can presented in medicament and medicated powder so that described microorganism wadding help solidifying Product can be suitably used for different guarantee the quality, using method, concentration, effect, outward appearance, packaging, movement requirement.
The microorganism wadding that the present invention provides helps solidifying product, originates from ocean wadding and helps solidifying microorganism, including being grown in marine environment In, the adverse circumstances containing radioactive substance are had higher tolerance, described microorganism wadding can be produced help the funguses of solidifying product, thin In bacterium, ancient bacterium, actinomycetes, cyanobacteria, slime bacteria, rickettsia, mycoplasma, chlamydia, spirillum, algae, protozoacide One or more.Described radioactive substance be alkali metal salt, alkali salt, lead salt, bismuth salt, boron salt, silicon salt, germanium salt, Arsenic, antimonic salt, tellurium salt, polonium salt, transition metal salt, group of the lanthanides salt, actinium series salt, alkane, alkene, alkynes, aromatic hydrocarbon and halogenated hydrocarbons, alcohol, phenol, Ether, aldehyde, ketone, carboxylic acid, ester one or more;And the described radioactive substance mass fraction concentration in water environment be 0~ 30%.Because these oceans wadding helps solidifying microorganism to live in for a long time in hypersaline environment, the microorganism wadding of production helps solidifying product toxic The adverse circumstances of evil property material also have higher tolerance, show preferable activity, can be widely used in multiple high salt organic Waste water, breaches traditional microbiological wadding and helps solidifying product to be difficult to keep the technology barriers of activity in high salt organic waste water.
That is, the present invention provide microorganism wadding help solidifying product, have resistance to extreme environment, can real-time monitoring, product The feature of form of diverse, improves performance and the scope of application that microorganism wadding helps solidifying product.
Second aspect, the invention provides a kind of microorganism wadding helps the preparation method of solidifying product, comprises the steps: to pass through Sea water medium separation obtains ocean wadding and helps solidifying microorganism, with ocean Zhong Shui, soil or sandstone as microbe carrier during being somebody's turn to do Microbiologic population carry out screening and culturing;Described ocean wadding is helped solidifying microorganism to carry out enrichment culture, with sea water training during being somebody's turn to do Foster base is microbial environment enrichment culture 2 ~ 3 days;Help solidifying product with the bacterium solution high-speed production microorganism wadding after described enrichment culture, Help the suspension of solidifying microorganism to be divided into two or more sets by being enriched with described ocean wadding during being somebody's turn to do, successively in turn every group of suspension is carried out Give the activation recovering process that the environmental impact of 1~24 hour processes, separates supernatant, carrying out 1~2 day in sea water medium Operation, quantified and rapidly produced the supernatant helping solidifying product containing described microorganism wadding according to batch cycle;Will be described Microorganism wadding help solidifying product to be concentrated or be dried to commercial specification, should during by detached containing described microorganism wad a quilt with cotton help solidifying The supernatant of product carries out concentrate formulation or processed powder, prepared product is dried.
In the further embodiment of the present invention, prepare described microorganism wadding and help the sea water employed in solidifying product process The composition of culture medium is sea water, soil, sandstone, agar, Carnis Bovis seu Bubali cream, peptone, yeast extract, starch, urea, saccharide, ammonium salt, sulphuric acid Salt, phosphate, nitrate, villaumite, alkali metal salt, alkali salt, transition metal salt, alkane, alkene, alkynes, aromatic hydrocarbon and halogenated hydrocarbons, Alcohol, phenol, ether, aldehyde, ketone, carboxylic acid, ester one or more.
In the further embodiment of the present invention, prepare described microorganism wadding and help the environment employed in solidifying product process Impact for salinity shock, temperature shock, hydraulic blow, oxygen content impact, the impact of nutritional labeling concentration, chemical substance impact, life One or more of thing antagonism impact.
In the further embodiment of the present invention, prepare described microorganism wadding and help the concentration employed in solidifying product process The method therefor of preparation be embrane method filtering and concentrating, evaporation and concentration, solid absorption concentration, gas absorption concentration, rotary-classify technology, One or more of electrochemistry concentration;The method therefor of described drying powder processed is low temperature spray drying, concentrate after evaporation drying, true Empty drying, lyophilization, pneumatic conveying drying, microwave drying, infrared drying, dielectric drying one or more.
The microorganism wadding that the present invention provides helps the preparation method of solidifying product, first by environmental impact process and microbial activity Recover technique to combine.It is by changing the environmental condition different from the most suitable culture environment of pathogenic microorganism, urging that environmental impact is processed Change the production technology accelerating Microbiological release microorganism wadding to help solidifying product;When ocean, wadding helps solidifying microorganism to be in extracellular environment drastically Under conditions of change, various biomacromolecules can be discharged in a large number in very short time, and this biomacromolecule has preferably Wadding effect of flocculant;In environmental impact procedures, produce microorganism wadding and help the speed of solidifying product can be controlled according to the degree of environmental impact System, preferred ambient impact degree be the former environmental optima factor (salinity, temperature, waterpower, oxygen content, nutritional labeling, chemical substance, Ant agonism) level 30%~300%, too low or too high use environment impact can lead to microorganism mortality, no Method completes follow-up microbial activity recovery process.Microbial activity recovers technique, is usually used in microbial engineering field, right In extreme circumstances inactivation but dead microorganism does not carry out activation processing, the present invention makes full use of microbial activity and recovers technique Advantage, it is combined with environmental impact treatment technology, enables the continual high speed of whole flow process, efficiently produces, to realize Microorganism wadding helps the volume production of solidifying product, simple production process, low cost.The wadding containing microorganism again being produced helps the supernatant of solidifying product Liquid energy is passed through concentrate formulation method or milling method is dried, and various product is obtained according to need, can directly encapsulate and sell, realize industry Change.
The microorganism wadding being provided using the present invention helps the preparation method of solidifying product, is separated by Marine microorganism, environment rushes Hit process, microbial activity recovery, concentrate formulation method and the technological process that processed powder is dried, microorganism wadding can be improved and help solidifying product Performance, the scope of application and production efficiency, reduce production difficulty and the production cost that microorganism wadding helps solidifying product.
The third aspect, the invention provides a kind of microorganism wadding helps the using method of solidifying product, comprises the steps: institute Stating microorganism wadding helps solidifying product to be combined with flocculation aid, is added in pending water body, stirring mixing 20~30 minutes;Standing is heavy Form sediment 2~4 hours, to realize coagulating sedimentation effect.
In the further embodiment of the present invention, the flocculation aid employed in solidifying product process is helped to be using microorganism wadding Calcium salt, chlorine, silicon salt, aluminium salt, iron salt, ferrous salt, magnesium salt, polyacrylamide, humic acid class, fulvic acid class, phenols aminoacid, sugar One or more of albumen, mucopolysaccharide, protein, cellulose, DNA (deoxyribonucleic acid).
In the further embodiment of the present invention, helping handled water body in solidifying product process using microorganism wadding is sea Water, surface water, subsoil water, production liquid, food processing wastewater, medical waste water, wastewater from chemical industry, dyeing waste water, municipal wastewater, rubbish One or more of rubbish percolate, brine waste, agricultural sewage, organic wastewater.
Because ocean wadding helps solidifying microorganism to live in for a long time in hypersaline environment, the microorganism wadding of production helps solidifying product toxic Also show higher activity in the adverse circumstances of evil property material, can be suitably used for process Various Complex water body and breach traditional micro- life Thing wadding helps solidifying product to be difficult to keep the technology barriers of activity in high salt organic waste water;And described microorganism wadding helps the solidifying product can be with Multiple flocculation aid combinations, have expanded performance and the scope of application of water process further.
The microorganism wadding being provided using the present invention helps the using method of solidifying product, helps solidifying product using described microorganism wadding High activity, by being combined flocculation aid, improves performance and the scope of application that microorganism wadding helps solidifying product.
Fourth aspect, the microorganism wadding that the present invention provides helps solidifying product processing fresh water, sea water, sewage and waste water, strengthening Embrane method bioreactor, the application in material separation.The microorganism wadding that prior art produces help solidifying product be from non-salt environment or The microniological proudcts that under salt environment, detached single culture produces, restricted application, and volume production difficulty are big, apply to extensive It is relatively costly that water body is processed, and the microorganism wadding of present invention preparation helps solidifying product, can be suitably used for the Different Waters of different quality, Due to achieving volume production technology, unit product is cheap, can be widely used in extensive water body process, embrane method bioreactor Strengthening and material detached application in.
The additional aspect of the present invention and advantage will be set forth in part in the description, and partly will become from the following description Obtain substantially, or recognized by the practice of the present invention.
Brief description
The flow chart that Fig. 1 helps the preparation method of solidifying product for the microorganism wadding in the embodiment of the present invention.
Fig. 2 is the three-dimensional fluorescence scanning optical spectrum figure that the microorganism wadding preparing in the embodiment of the present invention two helps solidifying product.
Fig. 3 is the three-dimensional fluorescence scanning optical spectrum figure that the microorganism wadding preparing in the embodiment of the present invention three helps solidifying product.
Fig. 4 is the three-dimensional fluorescence scanning optical spectrum figure that the microorganism wadding preparing in the embodiment of the present invention four helps solidifying product.
Fig. 5 is that the ocean wadding preparing in the embodiment of the present invention two, embodiment three and example IV helps solidifying microorganism to exist Bacterium colony photo in culture dish.
Fig. 6 is that in the embodiment of the present invention five, microorganism wadding helps solidifying product under different ph values, mariculture waste water to be mixed After solidifying precipitation process, contrast ph is turbidity standard when 7, and the ph value of drafting is schemed on the increase and decrease benefit percentage ratio impact of flocculation.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation description is it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.It is based on Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of not making creative work Embodiment, broadly falls into the scope of protection of the invention.
Embodiment one
The present embodiment provides a kind of microorganism wadding to help solidifying product, be ocean wadding help solidifying micro-organisms, there is flocculation and help solidifying Effect, the biomacromolecule presented in medicament or medicated powder, and have multiple Q-characters in three-dimensional fluorescence scanning optical spectrum Put (λ ex/ λ em), comprise 230/290 nanometer, 260/445 nanometer, 275/325 nanometer, one of 355/430 nanometer or several Individual;Described biomacromolecule be humic acid class, fulvic acid class, phenols aminoacid, glycoprotein, mucopolysaccharide, protein, cellulose, One or more of DNA (deoxyribonucleic acid).
Microorganism wadding for the present invention helps solidifying product, and the present invention devises the preparation side that this microorganism wadding helps solidifying product Method:
The flow chart that Fig. 1 helps the preparation method of solidifying product for the microorganism wadding in the embodiment of the present invention, as shown in figure 1, the present invention The microorganism wadding providing helps the preparation method of solidifying product, comprises the following steps:
S1: ocean wadding is obtained by sea water medium separation and helps solidifying microorganism, during being somebody's turn to do with ocean Zhong Shui, soil or sandstone be The microbiologic population of microbe carrier carries out screening and culturing;
S2: described ocean wadding is helped solidifying microorganism to carry out enrichment culture, rich for microbial environment with sea water medium during being somebody's turn to do Collection culture 2 ~ 3 days;
S3: help solidifying product with the bacterium solution high-speed production microorganism wadding after described enrichment culture, described ocean will be enriched with during being somebody's turn to do Wadding helps the suspension of solidifying microorganism to be divided into two or more sets, successively carries out giving the environment punching of 1~24 hour by every group of suspension in turn The operation that the activation recovering hit process, separating supernatant, carrying out in sea water medium 1~2 day is processed, is carried out according to batch cycle Quantify and rapidly produce the supernatant helping solidifying product containing described microorganism wadding;
S4: described microorganism wadding is helped solidifying product to be concentrated or is dried to commercial specification, by detached containing during being somebody's turn to do Stating microorganism wadding helps the supernatant of solidifying product to carry out concentrate formulation or processed powder, prepared product are dried.
The preparation method of solidifying product is helped to make furtherly with reference to the microorganism wadding that specific embodiment provides to the present invention Bright.
Embodiment two
Present embodiments provide the preparation method that a kind of microorganism wadding helps solidifying product, comprise the steps:
S1: weigh 1000 grams of sea water, 500 milligrams of peptones, 500 milligrams of phenol, 150 milligrams of dipotassium hydrogen phosphates, 50 milligrams of sulfur Sour magnesium, 25 milligrams of calcium chloride and 20 milligrams of ferrous sulfate, stirring is mixed to get sea water medium, then in this sea water medium Add 10 grams of sea muds, carry out cultivating bright to having in the environment of 30 degrees Celsius of temperature, dissolved oxygen content 2.5 mg/litre, ph value 7.5 Aobvious bacterium colony occurs, and then draws bacterium solution and is preserved, this microorganism identified belongs to arthrobacterium, and is gram positive bacteria;
S2: the ocean arthrobacterium bacterium solution drawing gained is added in 1 liter of sea water medium, in 30 degrees Celsius of temperature, dissolving Carry out enrichment culture 2 days in the environment of oxygen amount 2.5 mg/litre, ph value 7.5, obtain muddiness and contain bacteria suspension;
S3: be divided into two groups (then the volume of every group of bacterium solution is 0.5 liter) by muddy containing bacteria suspension, one group of muddiness contains bacteria suspension thereto Add 1 liter of tap water (then salinity is down to original 1/3rd), the salinity shock giving to stand 1 hour is processed, and separates and obtains Help the supernatant of solidifying product, remaining solid containing bacterium and suspension then to rejoin sea water medium to 0.5 liter containing microorganism wadding, The activation recovering carrying out 1 day is processed, and then carries out supernatant according to the circulation containing bacteria suspension muddy to this two groups of batch priority and (slightly produces Product) production and strain activation recovering;
S4: the supernatant of acquisition is carried out concentration to commercial specification, this process was carried out to 2 liters of supernatant using filter membrane Filter, 90% moisture is walked in filter, and remaining 200 milliliters of liquid are and help solidifying product with the microorganism wadding that medicine type exists.
Embodiment three
Present embodiments provide the preparation method that a kind of microorganism wadding helps solidifying product, comprise the steps:
S1: weigh 1000 grams of sea water, 1000 milligrams of hydroquinone, 400 milligrams of peptones, 120 milligrams of dipotassium hydrogen phosphates, 40 millis Gram magnesium sulfate, 20 milligrams of calcium chloride and 15 milligrams of ferrous sulfate, stirring is mixed to get sea water medium, then to this cultivation in sea water Add 25 grams of sea muds in base, carry out cultivating in the environment of 25 degrees Celsius of temperature, dissolved oxygen content 1.5 mg/litre, ph value 7 to having Substantially bacterium colony occurs, and then draws bacterium solution and is preserved, this microorganism identified belongs to marine protein bacterium, and is Gram-negative Bacterium;
S2: the bacterium solution drawing gained is added in 1 liter of sea water medium, in 25 degrees Celsius of temperature, dissolved oxygen content 1.5 milli G/l, carry out enrichment culture 3 days in the environment of ph value 7, obtain muddy containing bacteria suspension;
S3: be divided into four groups (then the volume of every group of bacterium solution is 0.25 liter) by muddy containing bacteria suspension, one group of muddiness is hanged containing bacterium thereto Liquid adds 0.25 liter of tap water (then salinity is down to original 1/2nd), and the salinity shock giving to stand 4 hours is processed, and separates Obtain the supernatant helping solidifying product containing microorganism wadding, remaining solid containing bacterium and suspension then rejoin sea water medium extremely 0.25 liter, the activation recovering carrying out 30 hours is processed, and then according to batch priority, this four groups muddinesses is circulated containing bacteria suspension on carrying out The production of clear liquid (crude product) and the activation recovering of strain;
S4: the supernatant of acquisition is carried out concentration to commercial specification, this process was carried out to 1 liter of supernatant using filter membrane Filter, 90% moisture is walked in filter, and remaining 100 milliliters of liquid are and help solidifying product with the microorganism wadding that medicine type exists.
Example IV
S1: weigh 2000 grams of sea water, 800 milligrams of catechols, 200 milligrams of peptones, 200 starch, 50 milligrams of ammonium chloride, 50 milligrams of dipotassium hydrogen phosphates, 30 milligrams of magnesium sulfate, 20 milligrams of calcium gluconate, stirring is mixed to get sea water medium, Ran Houxiang Add 50 grams of sea sands in this sea water medium, enter in the environment of 20 degrees Celsius of temperature, dissolved oxygen content 2 mg/litre, ph value 7.5 Row culture, to there being obvious bacterium colony to occur, is then drawn bacterium solution and is preserved, this microorganism identified belongs to vibrio marinopraesens, and is leather Lan Shi negative bacterium;
S2: by draw gained bacterium solution be added in 2 liters of sea water medium, 20 degrees Celsius of temperature, 2 milligrams of dissolved oxygen content/ Carry out enrichment culture 60 hours in the environment of liter, ph value 7.5, obtain muddiness and contain bacteria suspension;
S3: be divided into three groups (then the volume of every group of bacterium solution is 0.66 liter) by muddy containing bacteria suspension, change one of which muddiness and contain bacterium The ambient temperature of suspension to 25 degrees Celsius, dissolved oxygen content to 1.5 mg/litre, ph value to 7, the environment punching giving to stand 24 hours Hit process, separate and obtain the supernatant helping solidifying product containing microorganism wadding, remaining solid containing bacterium and suspension then rejoin sea To 0.66 liter, the activation recovering carrying out 2 days is processed water culture medium, then circulates containing bacteria suspension according to batch priority is muddy to this three groups Carry out the production of supernatant (crude product) and the activation recovering of strain;
S4: by the supernatant of acquisition be dried process to commercial specification, this process utilize spray drying device 60 degrees Celsius, Carry out low temperature spray drying, obtained 10 grams of pressed powders under conditions of negative pressure, helped with the microorganism wadding that powder form exists Solidifying product.
Solidifying product is helped according to the microorganism wadding that above-described embodiment one, embodiment two, embodiment three or example IV prepare Product, mainly show four feature locations (λ ex/ λ em) in three-dimensional fluorescence scanning optical spectrum in figure, represent four category feature things respectively Matter, including 230/290 nanometer of protein matter, 260/445 nanometer of fulvic acid class material, 275/325 nanometer of aminoacid Class material, 355/430 nanometer of humic acid class material.
The performance of solidifying product is helped to survey according to the microorganism wadding that above-described embodiment two, embodiment three or example IV prepare Fixed:
(1) measure the three-dimensional fluorescence scanning optical spectrum figure that described microorganism wadding helps effective ingredient in solidifying product
Fig. 2 is the three-dimensional fluorescence scanning optical spectrum figure that the microorganism wadding preparing in the embodiment of the present invention two helps solidifying product;
Fig. 3 is the three-dimensional fluorescence scanning optical spectrum figure that the microorganism wadding preparing in the embodiment of the present invention three helps solidifying product;
Fig. 4 is the three-dimensional fluorescence scanning optical spectrum figure that the microorganism wadding preparing in the embodiment of the present invention four helps solidifying product.
Show in the described three-dimensional fluorescence scanning optical spectrum figure recording with the inventive method, prepare in embodiment two Microorganism wadding helps solidifying product mainly to have two feature locations (representative feature material), including 260/445 nanometer of (fulvic acid class thing Matter) and 355/430 nanometer (humic acid class material);The microorganism wadding preparing in embodiment three helps solidifying product mainly to have one Feature locations, are 230/290 nanometer (protein matter);The microorganism wadding preparing in example IV helps solidifying product mainly to have One feature locations, is 275/325 nanometer (amino acidses material).
In the present invention, due to the presence of described feature locations, can achieve the reality to coagulation water body with fluorescent scanning instrument When monitoring, realize digitized, remote-control, intelligent water chemical treatment.It should be noted that due to three-dimensional fluorescence scanning optical spectrum in figure Feature locations can help composition and concentration, the software and hardware of measuring instrument and parameter setting, the measurement ring of solidifying product with microorganism wadding The change of border and condition and change, then the feature locations of three-dimensional fluorescence scanning optical spectrum in figure are not restricted to enumerate in the present invention Feature locations, all using based on the inventive method, approximate the inventive method but do not make innovations creation, be used for counting Other feature locations of the described three-dimensional fluorescence scanning optical spectrum in figure of wordization monitoring are all within protection scope of the present invention;And institute State the species that biomacromolecule is also not restricted to enumerate in the present invention, enumerate in all present invention the derivant of composition, coordination compound, Chelate, trim, polymer and with the composition of the artificial mixture of other materials all within protection scope of the present invention.By In these biomacromolecules, there is flocculation and Coagulation aiding, can be used alone, be combined, assisting to be used in all kinds of water treatment technologies In, improve microorganism wadding and help performance and the scope of application of solidifying product, and can be so that described presented in medicament and medicated powder Microorganism wadding help solidifying product can be suitably used for different guarantee the quality, using method, concentration, effect, outward appearance, packaging, movement requirement.
(2) ocean wadding helps the bacterium colony appearance of solidifying microorganism
Fig. 5 is that the ocean wadding preparing in the embodiment of the present invention two, embodiment three and example IV helps solidifying microorganism in culture Bacterium colony photo in ware.
Bacterium colony photo in culture dish for the solidifying microorganism is helped to show in the ocean wadding with the inventive method record, embodiment In two, the bacterium colony of detached ocean arthrobacterium is yellow;In embodiment three, the bacterium colony of detached marine protein bacterium is white;Implement In example four, the bacterium colony of detached vibrio marinopraesens is white.It should be noted that the bacterium colony of microorganism can with cell quantity, kind, The difference of gene and environment and there are different sign appearance, then described ocean wadding helps the sign appearance of solidifying microorganism to be not intended to limit The sign appearance enumerated in the present invention, all using based on the inventive method or approximate the inventive method but do not make innovation Property innovation and creation and detached microbe colony other characterize appearance also all within protection scope of the present invention.
(3) microorganism wadding helps the flocculation ability of solidifying product
By spectrophotometric determination, described microorganism wadding helps solidifying product fine to the flocculating effect of aqueous suspension ofkaolin, turbidity Clearance is 50%~60%, and the turbidity removal after cooperation calcium chloride flocculation aid can reach 85%~95%;Described microorganism wadding helps solidifying Product is to the Organic substance (cod in aqueous suspension ofkaolinmn) also can reach 20%~40% clearance, cooperation calcium chloride flocculation aid make With rear Organic substance in aqueous suspension ofkaolin can be reached 60%~80% clearance;Described microorganism wadding helps solidifying product to kaolinite Heavy metal ion in native suspension and other inorganic salts can reach 10%~30% clearance, and cooperation calcium chloride flocculation aid uses Afterwards the heavy metal ion in aqueous suspension ofkaolin and other inorganic salts can be reached with 30%~50% clearance.This microorganism wadding helps Solidifying product helps solidifying microorganism due to originating from ocean wadding, and dirt organic to various high salt, waste water all have certain resistance, can be in severe ring Still show higher activity in border, improve performance and the range that microorganism wadding helps solidifying product.
The microorganism wadding that the embodiment of the present invention two to example IV prepares helps solidifying product, has broad applicability, energy Process for multiple lives and industrial water body;Production difficulty is low, has general applicability, all can use this all over the world Bright method carry out efficiently, the production of high speed;Finished product can also can be sold presented in medicament or medicated powder after production.
In the present invention, described ocean wadding helps solidifying microorganism to be to be grown in marine environment, to the evil containing radioactive substance Funguses that bad environment has higher tolerance, described microorganism wadding can be produced helping solidifying product, antibacterial, ancient bacterium, actinomycetes, blue thin One or more of bacterium, slime bacteria, rickettsia, mycoplasma, chlamydia, spirillum, algae, protozoacide;Described poison Evil property material is alkali metal salt, alkali salt, lead salt, bismuth salt, boron salt, silicon salt, germanium salt, arsenic, antimonic salt, tellurium salt, polonium Salt, transition metal salt, group of the lanthanides salt, actinium series salt, alkane, alkene, alkynes, aromatic hydrocarbon and halogenated hydrocarbons, alcohol, phenol, ether, aldehyde, ketone, carboxylic acid, ester One or more, and mass fraction concentration in water environment for the described radioactive substance is 0~30%.It should be noted that being used for Produce microorganism wadding and help the ocean of solidifying product to flocculate and help microorganism to be not restricted to the above-mentioned species enumerated, all using based on this Inventive method, approximate the inventive method but do not make innovations creation, microorganism wadding can be produced and help micro- life of solidifying product Thing is all within protection scope of the present invention.
It is notable that entering from sea mud during helping solidifying microorganism with the inventive method separating marine wadding Row microorganism detached efficiency highest, higher than using other microbe carriers in ocean, simultaneously in the present invention ocean wadding help solidifying The source of microorganism should be interpreted broadly, including the soil in ocean, water body, sandstone, rock stratum, animal and plant body, ship, artificial drift Float etc.;Soil after nature or artificial action migrate to land for the material, water body, sandstone, rock stratum, animals and plants in ocean Body, ship, artificial float;Other are as similar environments such as artificial ocean environment, class ocean water body, the Gao Han of microbe carrier Body of salt and other saline environment, preparation microorganism wadding helps and can be selected for during solidifying product in the above-mentioned microbe-derived environment enumerated One or more.It should be noted that helping solidifying microorganism to prepare micro- life that microorganism wadding helps solidifying product for separating marine wadding Thing carrier environment is not restricted to the above-mentioned species enumerated, and all separating marine waddings that can be used for help solidifying microorganism to prepare microorganism Wadding helps the microbe carrier environment of solidifying product all within protection scope of the present invention.
It should be noted that the performance that the microorganism wadding preparing with the parameter setting of embodiment two helps solidifying product is Good, it is also feasible and efficient that the microorganism wadding that certainly other embodiments prepare helps solidifying product.Except embodiment two Situation about enumerating to example IV, using other oceans wadding help solidifying microorganism and its sea water medium, microbe carrier environment, Screening and enrichment culture environment, production microorganism are wadded a quilt with cotton and are helped packet count, environmental impact condition and time, the activation recovering bar of solidifying product Part and the preparation after time, crude product separation and flouring technology are also feasible.
In the present invention, the composition of described sea water medium is sea water, soil, sandstone, agar, Carnis Bovis seu Bubali cream, peptone, ferment Female cream, starch, urea, saccharide, ammonium salt, sulfate, phosphate, nitrate, villaumite, alkali metal salt, alkali salt, transition metal Salt, alkane, alkene, alkynes, aromatic hydrocarbon and halogenated hydrocarbons, alcohol, phenol, ether, aldehyde, ketone, carboxylic acid, ester one or more.It should be noted that institute State sea water medium and be not restricted to the above-mentioned composition enumerated, all saline, solonchak, the class marine environment of artificial or self-assembling formation And the material in saline environment, the Organic substance being added in sea water medium and inorganic salts ingredients are all in the protection model of the present invention Within enclosing.
In the present invention, it is grouped first Posterior circle and carries out environmental impact and the operation of activation recovering, be to ensure that efficient, high fast-growing Producing microbial wadding helps the core process of solidifying product.With the initial value of the single environment factor for 100%, in the process of environmental impact In, described environmental impact that this envirment factor is brought answers advantageous variant to the 30 ~ 300% of initial value, this is because this environment because When being changed to less than 30% or being higher than 300% of son, the environmental impact that Institute of Micro-biology bears is excessive, and microorganism can be led to dead in a large number Die, affect follow-up activation recovering process;If but envirment factor change to initial value 70 ~ 150% when, environmental impact Dynamics is too little, produces microorganism wadding and helps the speed of solidifying product too slow, does not reach the purpose of high-speed production;Using multiple environment because During son change gives microbial environment impact, should be superimposed and consider and calculate the environmental impact that each envirment factor is brought, protect Demonstrate,prove total envirment factor to change the 30 ~ 300% of total initial value, it is to avoid too high environmental impact leads to microorganism mortality. And the operation of activation recovering is to ensure that this sustainable production efficient, that high-speed production microorganism wadding helps solidifying Product Process, be conducive to Reduces cost, energy-saving and emission-reduction;It should be noted that activation recovering operates the reverse bioprocess for environmental impact operation, then activity The time of recovery operation should be arranged according to the degree of environmental impact, when total envirment factor change is in the initial number of original initial numerical value Value 50 ~ 200% when, activation recovering operation time should be arranged on 1 day about;When total envirment factor changes in original initial number Value initial value 30 ~ 300% when, activation recovering operation time should be arranged on 2 days about.
In the present invention, environmental impact be salinity shock, temperature shock, hydraulic blow, oxygen content impact, nutritional labeling dense One or more that degree impact, chemical substance are impacted, Ant agonism impacts, can be by changing salt content, ambient temperature, stirring Mix and current dynamics, water oxygen content, water in the means such as composition and concentration, ecosystem environment realizing.It should be mentioned that It is, for accelerating microorganism wadding described in micro-organisms to help the environmental impact method of solidifying product to be not restricted to the above-mentioned kind enumerated Class, all environmental impacts promoting microorganism generation described microorganism wadding to help solidifying product by change envirment factor are all at this Within bright protection domain.
In the present invention, the method therefor of described concentrate formulation be embrane method filtering and concentrating, evaporation and concentration, solid absorption dense Contracting, gas absorption concentration, rotary-classify technology, electrochemistry concentrate one or more;The method therefor of described drying powder processed is Evaporation drying, vacuum drying, lyophilization, pneumatic conveying drying, microwave drying, infrared drying, height after low temperature spray drying, concentration Frequently one or more being dried.It should be noted that can with preparation and the method for powder processed be not restricted to above-mentioned enumerating Species, what all energy used can help solidifying Product processing for the additive method of preparation and powder processed also in the present invention on described microorganism wadding Protection domain within.The supernatant liquid energy being helped solidifying product by producing the wadding containing microorganism passes through concentrate formulation method or drying Milling method, is obtained various product according to need, can directly encapsulate and sell, realize industrialization.
The microorganism wadding being provided using the present invention helps the preparation method of solidifying product, is separated by Marine microorganism, environment rushes Hit process, microbial activity recovery, concentrate formulation method and the technological process that processed powder is dried, microorganism wadding can be improved and help solidifying product Performance, the scope of application and production efficiency, reduce production difficulty and the production cost that microorganism wadding helps solidifying product.
Microorganism wadding for the present invention helps solidifying product, and the present invention devises the user that this microorganism wadding helps solidifying product Method, comprises the following steps:
S5: described microorganism wadding is helped solidifying product to be combined with flocculation aid, is added in pending water body, stir mixing 20~30 Minute;
S6: staticly settle 2~4 hours, to realize coagulating sedimentation effect.
The using method of solidifying product is helped to make furtherly with reference to the microorganism wadding that specific embodiment provides to the present invention Bright.
Embodiment five
Present embodiments provide the using method that a kind of microorganism wadding helps solidifying product, comprise the steps:
S5: the microorganism wadding that 100 milliliters of ocean arthrobacteriums are produced helps solidifying product formulation to mix with 0.1 gram of calcium chloride, and is divided into Four parts, be added to respectively ph value be 6, in 7,8,91 liter of muddy mariculture waste water (high salt organic waste water), stirring mixing 25 minutes;
S6: staticly settle 3 hours, complete precipitation process of coagulation, after solid-liquid separation, discharge upper strata clear water.
The microorganism wadding of preparation helps solidifying product that the coagulation performance of actual sewage is measured:
According to the processing method of embodiment five, measure the indices of the water outlet processing gained, when ph is 7, its turbidity goes Except rate is 60%, Organic substance (codmn) clearance be 35%, inorganic salt clearance be 20%.
Fig. 6 is that in the embodiment of the present invention five, microorganism wadding helps solidifying product under different ph values, mariculture waste water to be mixed After solidifying precipitation process, contrast ph is turbidity standard when 7, and the ph value of drafting is schemed on the increase and decrease benefit percentage ratio impact of flocculation.
Affect result from the beneficial percentage ratio of the increase and decrease on flocculation it is found that the ph value of water body affects relatively on coagulation effect Greatly, when ph value is for 6, declining profit percentage ratio reaches -40%, i.e. 20% turbidity removal rate;When ph value is for 8, gain percentage ratio reaches Arrive+15%, i.e. 75% turbidity removal rate;When ph value is for 9, gain percentage ratio reaches+30%, i.e. 90% turbidity removal rate.Need It is noted that turbidity removal rate is the core index of coagulation effect detection, but help solidifying product treatment with described microorganism wadding The coagulation effect that water body is reached is not limited only to this index of turbidity removal rate, and all energy characterize described microorganism wadding and help solidifying product Coagulation performance index all within protection scope of the present invention.
When the microorganism wadding providing using the present invention helps the using method of solidifying product, pending water sample should be measured in advance Ph value, operating condition allow in the case of, carry out coagulating treatment under weak basic condition as far as possible, with improve microorganism wadding help solidifying The water treatmenting performance of product.
Embodiment six
Present embodiments provide the using method that a kind of microorganism wadding helps solidifying product, comprise the steps:
S5: the microorganism wadding that 1 gram of ocean arthrobacterium is produced helps solidifying product powder to mix with 0.2 gram of calcium chloride, is added to ph value In 7.5 10 liters of muddy saliferous leather-making waste waters, stirring mixing 30 minutes;
S6: staticly settle 2.5 hours, complete precipitation process of coagulation, after solid-liquid separation, discharge upper strata clear water.
Embodiment seven
Present embodiments provide the using method that a kind of microorganism wadding helps solidifying product, comprise the steps:
S5: the microorganism wadding that 20 milliliters of marine protein bacterium are produced helps solidifying product formulation to mix with 50 milligrams of calcium chloride, is added to 1.5 liters of muddy flushing the toilet in sanitary sewage of ph value 8, stirring mixing 20 minutes;
S6: staticly settle 2 hours, complete precipitation process of coagulation, after solid-liquid separation, discharge upper strata clear water.
It should be noted that situation about enumerating to embodiment seven except embodiment five, solidifying product is helped using described microorganism wadding It is also feasible that product are combined other flocculation aids.In the using method that the microorganism wadding of the present invention helps solidifying product, adopted helps Solidifying agent can be calcium salt, chlorine, silicon salt, aluminium salt, iron salt, ferrous salt, magnesium salt, polyacrylamide, humic acid class, fulvic acid class, phenols One or more of aminoacid, glycoprotein, mucopolysaccharide, protein, cellulose, DNA (deoxyribonucleic acid).It should be noted that energy Combination microorganism wadding helps the flocculation aid species of solidifying product to be not restricted to the above-mentioned species enumerated, and all can be used for helping of water process Solidifying agent is all within protection scope of the present invention.
In the present invention, handled water body in solidifying product process is helped to include sea water, surface water, underground using microorganism wadding Water, production liquid, food processing wastewater, medical waste water, wastewater from chemical industry, dyeing waste water, municipal wastewater, percolate, saliferous One or more of waste water, agricultural sewage, organic wastewater.Because ocean wadding helps solidifying microorganism to live in hypersaline environment for a long time In, the microorganism wadding of production helps solidifying product also to show higher activity in the adverse circumstances containing radioactive substance, can be suitable for Breaching traditional microbiological wadding in process Various Complex water body helps solidifying product to be difficult to keep the skill of activity in high salt organic waste water Art barrier;And described microorganism wadding helps solidifying product can be combined with multiple flocculation aids, expand the performance of water process further and fitted Use scope.
Embodiment eight
The present embodiment provides microorganism wadding and helps solidifying product processing fresh water, sea water, sewage and waste water, and strengthening embrane method biology is instead Answer device, the application in material separation.It is from lower point of non-salt environment or salt environment that the microorganism wadding that prior art produces helps solidifying product From the microniological proudcts that produce of single culture, restricted application, and volume production difficulty is big, applies to what extensive water body was processed Relatively costly, and the microorganism wadding of present invention preparation helps solidifying product, can be suitably used for the Different Waters of different quality, due to achieving Volume production technology, unit product is cheap, can be widely used in extensive water body and process;The microorganism wadding that the present invention produces again helps , due to can be polymerized cotton-shaped activated sludge by coagulation, formation ecosystem is more stable, the more preferable seed activity of effect of settling is dirty for solidifying product Mud, can apply to strengthen the water treatmenting performance of activated sludge, and strengthening embrane method bioreactor, reduction membrane fouling rate;And by Help solidifying product degradable, pollution-free in described microorganism wadding, can be used in the detached application of many kinds of substance.
Although embodiments of the invention have been shown and described above it is to be understood that above-described embodiment is example Property it is impossible to be interpreted as limitation of the present invention, those of ordinary skill in the art within the scope of the invention can be to above-mentioned Embodiment is changed, changes, replacing and modification, and does not make the essence of appropriate technical solution depart from various embodiments of the present invention skill The scope of art scheme, it all should be covered in the middle of the claim of the present invention and the scope of description.

Claims (10)

1. a kind of microorganism wadding help solidifying product it is characterised in that: it is to wad a quilt with cotton and help solidifying microorganism in ocean that described microorganism wadding helps solidifying product Producing, there is flocculation and Coagulation aiding, the biomacromolecule presented in medicament or medicated powder, and sweep in three-dimensional fluorescence Retouch and in spectrum, have multiple feature locations (λ ex/ λ em), comprise 230/290 nanometer, 260/445 nanometer, 275/325 nanometer, 355/ One of 430 nanometers or several;Described biomacromolecule is humic acid class, fulvic acid class, phenols aminoacid, glycoprotein, viscous One or more of polysaccharide, protein, cellulose, DNA (deoxyribonucleic acid).
2. microorganism according to claim 1 wadding help solidifying product it is characterised in that: described ocean wadding helps solidifying microorganism to make a living Length have in marine environment, to the adverse circumstances containing radioactive substance higher tolerance, can produce described microorganism and wad a quilt with cotton help solidifying The funguses of product, antibacterial, ancient bacterium, actinomycetes, cyanobacteria, slime bacteria, rickettsia, mycoplasma, chlamydia, spirillum, algae One or more of class, protozoacide;Described radioactive substance be alkali metal salt, alkali salt, lead salt, bismuth salt, boron salt, Silicon salt, germanium salt, arsenic, antimonic salt, tellurium salt, polonium salt, transition metal salt, group of the lanthanides salt, actinium series salt, alkane, alkene, alkynes, aromatic hydrocarbon and halogen For hydrocarbon, alcohol, phenol, ether, aldehyde, ketone, carboxylic acid, ester one or more;And the described radioactive substance mass fraction in water environment Concentration is 0~30%.
3. microorganism wadding according to claim 1 helps the preparation method of solidifying product it is characterised in that comprising the steps: Ocean wadding is obtained by sea water medium separation and helps solidifying microorganism, with ocean Zhong Shui, soil or sandstone as microorganism during being somebody's turn to do The microbiologic population of carrier carries out screening and culturing;Described ocean wadding is helped solidifying microorganism to carry out enrichment culture, with sea during being somebody's turn to do Water culture medium is microbial environment enrichment culture 2 ~ 3 days;Helped solidifying with the bacterium solution high-speed production microorganism wadding after described enrichment culture Product, helps the suspension of solidifying microorganism to be divided into two or more sets by being enriched with described ocean wadding during being somebody's turn to do, successively hangs every group in turn The activity that the environmental impact that liquid carries out giving 1~24 hour processes, separates supernatant, carry out 1~2 day in sea water medium is extensive The multiple operation processing, is quantified and is rapidly produced the supernatant helping solidifying product containing described microorganism wadding according to batch cycle; Described microorganism wadding is helped solidifying product to be concentrated or is dried to commercial specification, by detached containing described microorganism during being somebody's turn to do Wadding helps the supernatant of solidifying product to carry out concentrate formulation or processed powder, prepared product are dried.
4. microorganism according to claim 3 wadding help solidifying product preparation method it is characterised in that: described sea water medium Composition be sea water, soil, sandstone, agar, Carnis Bovis seu Bubali cream, peptone, yeast extract, starch, urea, saccharide, ammonium salt, sulfate, phosphorus Hydrochlorate, nitrate, villaumite, alkali metal salt, alkali salt, transition metal salt, alkane, alkene, alkynes, aromatic hydrocarbon and halogenated hydrocarbons, alcohol, Phenol, ether, aldehyde, ketone, carboxylic acid, ester one or more.
5. microorganism according to claim 3 wadding help solidifying product preparation method it is characterised in that: described environmental impact is Salinity shock, temperature shock, hydraulic blow, oxygen content impact, the impact of nutritional labeling concentration, chemical substance impact, biological antagonist One or more of impulses.
6. microorganism according to claim 3 wadding help solidifying product preparation method it is characterised in that: described concentrate formulation Method therefor is embrane method filtering and concentrating, evaporation and concentration, solid absorption concentration, gas absorption concentration, rotary-classify technology, electrochemistry One or more concentrating;The method therefor of described drying powder processed is low temperature spray drying, concentrate after evaporation drying, vacuum do Dry, lyophilization, pneumatic conveying drying, microwave drying, infrared drying, dielectric drying one or more.
7. microorganism wadding according to claim 1 helps the using method of solidifying product it is characterised in that comprising the steps: Described microorganism wadding is helped solidifying product to be combined with flocculation aid, is added in pending water body, stirring mixing 20~30 minutes;Quiet Put precipitation 2~4 hours, to realize coagulating sedimentation effect.
8. microorganism according to claim 7 wadding help solidifying product using method it is characterised in that: described flocculation aid is calcium Salt, chlorine, silicon salt, aluminium salt, iron salt, ferrous salt, magnesium salt, polyacrylamide, humic acid class, fulvic acid class, phenols aminoacid, sugared egg In vain, one or more of mucopolysaccharide, protein, cellulose, DNA (deoxyribonucleic acid).
9. microorganism according to claim 7 wadding help solidifying product using method it is characterised in that: described pending water Body is sea water, surface water, subsoil water, production liquid, food processing wastewater, medical waste water, wastewater from chemical industry, dyeing waste water, municipal administration One or more of sewage, percolate, brine waste, agricultural sewage, organic wastewater.
10. microorganism wadding according to claim 1 helps solidifying product processing fresh water, sea water, sewage and waste water, strengthens embrane method Bioreactor, the application in material separation.
CN201610897656.7A 2016-10-16 2016-10-16 Microbiological product capable of flocculation and coagulation enhancement and preparation method, use method and application thereof Pending CN106365279A (en)

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Application publication date: 20170201