CN106350567A - Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane - Google Patents

Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane Download PDF

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CN106350567A
CN106350567A CN201610727806.XA CN201610727806A CN106350567A CN 106350567 A CN106350567 A CN 106350567A CN 201610727806 A CN201610727806 A CN 201610727806A CN 106350567 A CN106350567 A CN 106350567A
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culture
propionic acid
methane
anaerobic
acid
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黄艳
马诗淳
凡慧
邓宇
张敏
施国中
王春芳
尹小波
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Biogas Institute of Ministry of Agriculture
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Biogas Institute of Ministry of Agriculture
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C02F11/04—Anaerobic treatment; Production of methane by such processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023—Methane
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/59—Biological synthesis; Biological purification

Abstract

The invention provides a method for promoting the starting of an anaerobic digestor and accelerating the conversion of propionic acid into methane. The method comprises the steps of preparing compound bacteria, adding a solid carrier into the compound bacteria in a mass percentage of 1%-20%, inoculating the compound bacteria into a sterile anaerobic BCTY culture medium containing 1500ppm-10000ppm of sodium propionate, and carrying out anaerobic standing culturing at 40 DEG C, wherein the pH value of the sterile anaerobic BCTY culture medium is 7.5-8.5. By virtue of the method, the degradation rate of propionic acid can be substantially increased, and the time required for the complete degradation of propionic acid can be substantially shortened; the operation efficiency of the metabolic conversion of propionic acid into methane is remarkably promoted; and the transport metabolism of H2 in an anaerobic digestion system can be effectively promoted, so that the anaerobic digestion system is maintained at a relatively low hydrogen partial pressure state, is relatively stable and has relatively strong inversion resistance, and the operation load is substantially improved.

Description

A kind of promotion anaeroic digestor starts and accelerates the method that propionic acid is methane
Technical field
The invention belongs to field of fermentation engineering is and in particular to a kind of promotion anaeroic digestor starts and acceleration propionic acid is The method of methane.
Background technology
In anaerobic System, the accumulation of the organic acid such as acetic acid, propanoic acid, butanoic acid often results in anaerobic digester system acidifying, has Machine load reduction or even whole system collapse.However, as the important mesostate of organic waste anaerobism conversion, second Acid, propanoic acid, butanoic acid are but difficult to degrade.Short-chain fatty acid (as second, third and butanoic acid) is the important middle generation in marsh gas fermentation processes Thank to product, its metabolic conversion is the important step controlling marsh gas fermentation processes.But at standard conditions, the anaerobism of these fatty acids Oxidation reaction is an endothermic reaction (Gibbs free energy δ g > 0) it is impossible to spontaneous generation.There is no other electron acceptor conditions Under, the degraded of short-chain fatty acid needs to be coupled with methane phase Gu bacterium by fatty acid oxidation bacterium, by syntrophism metabolism ability Complete.Microorganism syntrophism metabolism is the rate-limiting step during organic matter degradation methane phase, participates in the syntrophism bacterium battalion of this step metabolism Foster condition is harsh, energy that is obtaining is low, poor growth, significantly limit the metabolism of fatty acid.Additionally, in syntrophism metabolic process In, must be set up good material, energy transmission between fatty acid oxidation bacterium and methane phase Gu bacterium, just enable syntrophism metabolism It is smoothed out.
The degraded of propanoic acid needs more energy and lower hydrogen dividing potential drop, therefore than the more difficult degradation of other short-chain fatty acid. Therefore, the homergy controlling propanoic acid is also the key issue maintaining marsh gas fermentation processes to be normally carried out.
Content of the invention
For the shortcoming of prior art, it is an object of the invention to provide a kind of promote anaeroic digestor to start and accelerate third The method that acid is converted into methane, the method comprises the steps:
(1) preparation of compound bacteria:
1) with dextran wastewater anaerobic digestive device water outlet as initial inoculum, using sodium propionate as carbon source, inoculated Cultivate and reach 50-80% to propionic acid degradation rate in culture, methane content is 30-50%；
2) by step 1) gains transfer to fresh culture culture, and press every ml step 1) culture fluid, add thin as follows Bacterium:
3) by step 2) gains carry out sealing culture, obtain final product the compound bacteria of described Rapid reversal propionic acid accumulation；
(2) prepare fresh culture, add the sodium propionate of final concentration of 3000mg/l, inoculation 10% step (1) gained is multiple Close bacterium, 40 DEG C of quiescent culture reach 50-80% to propionic acid degradation rate, methane content is 30-50%；
(3) described for step (2) culture is transferred to fresh culture, is added with the ratio of mass percent 1-20% solid Type carrier, described curable type carrier includes the biocycle for 6-15mm for the internal diameter, particle diameter is 50-250 μm hollow glass micropearl, particle diameter Activated carbon for 1-5mm, the solid pv ball of a diameter of 5-12mm, bulk density are 0.1-0.2g/cm3Activated carbon felt in one Kind；
(4) by step (3) gains sealing simultaneously quiescent culture, until propionic acid degradation rate reaches 70-100%, as complete third The start-up course of sour anaerobic digester system；The sodium propionate of continuation interpolation 1-30g/l after the completion of the startup of propanoic acid anaerobic digester system, 40 DEG C quiescent culture, as propanoic acid anaerobic digester system start after running.
In step 1) inoculation when, inoculum concentration be 1-20%, condition of culture be under 25-55 DEG C, ph=6.0-9.0 sealing training Support.
Preferably, the addition of sodium propionate is 1500-10000mg/l.
Described culture medium, based on every liter of culture medium, including 1g nh4Cl, 1g yeast powder, 1g tryptone, 0.5g half Guang ammonia Acid hydrochloride, 50ml a great number of elements solution, 10ml trace element solution, 10ml Thioctic Acid 1,10mg "diazoresorcinol"；Described a large amount of Every liter of inclusion 6g kh of element2po4, 12g nacl, 2g mgcl2·6h2O, 1.6g cacl2·2h2o；Described trace element solution Every liter of inclusion 12.8g aminotriacetic acid, 1.35g fecl3·6h2O, 0.1g mncl2·4h2O, 0.1g cocl2·6h2O, 0.1g zncl2, 0.025g cucl2·2h2O, 0.01g h3bo3, 0.024g na2moo4·2h2O, 1g nacl, 0.12g nicl2·6h2O, 0.026g na2seo3·5h2o.
Step 3) in condition of culture be: cultivation temperature be 25-55 DEG C, ph be 6.0-9.0.
Preferably, described curable type carrier is the activated carbon of 1-5mm for particle diameter or hollow glass that particle diameter is 50-250 μm is micro- Pearl.It is furthermore preferred that described curable type carrier is the activated carbon of 1-5mm for particle diameter.
Beneficial effects of the present invention:
1st, the present invention can be greatly improved the degradation rate of propanoic acid and significantly shorten the propanoic acid degradable required time；
2nd, the present invention has significant facilitation to the operational efficiency of propanoic acid metabolic conversion methane；
3rd, the present invention can effectively facilitate h in anaerobic digester system2Transhipment metabolism, make system maintain relatively low hydrogen dividing potential drop , so that anaerobic digester system is more stable, degeneration-resistant row is higher for state, significantly lifts operating load.
Brief description
Fig. 1 is the hydrolysis ability figure to propanoic acid for the compound bacteria of the present invention；
Fig. 2 is the adaptability figure to ph for the composite flora；
Fig. 3 tries hard to the metabolizable energy of sodium propionate for composite flora；
The impact that Fig. 4 and Fig. 5 aoxidizes to propanoic acid syntrophism for hollow glass micropearl；
Fig. 6 promotes the quick design sketch starting of propanoic acid anaerobic digester system for curable type carrier；
Fig. 7 accelerates the methanogenic design sketch of propanoic acid anaerobic digester system propionic acid for curable type carrier；
Fig. 8 is the impact result figure to propanoic acid syntrophism metabolism for the different curable type vector contgs, and wherein, a, b represent propanoic acid syntrophism The propionic acid degradation rate of metabolism startup stage (1st) and methane phase situation, c, d represent propanoic acid syntrophism metabolism operation phase (2st) Propionic acid degradation rate and methane phase situation；
Fig. 9 is the impact result figure to hydrogen dividing potential drop in anaerobic digester system and operating load for the curable type carrier of the present invention.
Specific embodiment
Below by embodiment, the present invention is specifically described it is necessary to it is pointed out here that be following examples be use In being further detailed it is impossible to be interpreted as limiting the scope of the invention to the present invention, being skilled in technique of this field Personnel made according to foregoing invention content some nonessential improve and adjust, still fall within protection scope of the present invention.
The formula of bcty culture medium used in following embodiments is: based on every liter of culture medium, by 1g nh4Cl, 1g yeast Powder, 1g tryptone, 0.5g cysteine hydrochloride, 50ml a great number of elements solution, 10ml trace element solution, 10ml vitamin 141,10mg "diazoresorcinol"s；Described every liter of inclusion 6g kh of a great number of elements2po4, 12g nacl, 2g mgcl2·6h2O, 1.6g cacl2·2h2o；Described every liter of inclusion 12.8g aminotriacetic acid of trace element solution, 1.35g fecl3·6h2O, 0.1g mncl2·4h2O, 0.1g cocl2·6h2O, 0.1g zncl2, 0.025g cucl2·2h2O, 0.01g h3bo3, 0.024g na2moo4·2h2O, 1g nacl, 0.12g nicl2·6h2O, 0.026g na2seo3·5h2O forms.
Embodiment 1
Prepare the bcty culture fluid of anaerobism, add the sodium propionate of final concentration of 3000mg/l, subpackage 150ml detests in 250ml In oxygen bottle, inoculate the wastewater anaerobic digestive device water outlet of 10% (v/v) dextran, sealing, 40 DEG C of static gas wave refrigerator.Treat propionic acid degradation rate Reach 80%, methane content is 50% about, according to following three kinds of schemes interpolation function stem (individual cell/ml culture fluid):
Scheme one: pelotomaculum schinkii 2.5 × 107, pelotomaculum propionicicum 1 ×107, syntrophobacter wolinii 1 × 106, methanospirillum hungatei 4 × 106, methanoculleuspalmolei 5×106, methanoculleus bourgensis 5 × 106, methanosarcina barkeri 5×106, methanosarcina mazei 1 × 107.
Scheme two: pelotomaculum schinkii 4 × 107, pelotomaculum propionicicum 1.5 ×107, syntrophobacter wolinii 3 × 106, methanospirillum hungatei 1.4 × 107, methanoculleuspalmolei 1×107, methanoculleus bourgensis 1 × 107, methanosarcina barkeri 1.5×107, methanosarcina mazei 2 × 107.
Scheme three: pelotomaculum schinkii 5 × 107, pelotomaculum propionicicum 2.5 ×107, syntrophobacter wolinii 3 × 106, methanospirillum hungatei 1.4 × 107, methanoculleuspalmolei 1.4×107, methanoculleus bourgensis 1.4 × 107, methanosarcina barkeri 2.5×107, methanosarcina mazei 3 × 107；
Sealing, 40 DEG C of static gas wave refrigerator, monitoring propanoic acid fall concentration and methane production, result is shown in Fig. 1.
The adaptability to ph for embodiment 2 composite flora
Prepare the bcty culture fluid of anaerobism, add the sodium propionate of final concentration of 3000mg/l, subpackage 150ml detests in 250ml In oxygen bottle, inoculate the wastewater anaerobic digestive device water outlet of 20% (v/v) dextran, sealing, 20 DEG C of static gas wave refrigerator.Treat propionic acid degradation rate Reach 70%, methane content is 40% about, according to below scheme interpolation function stem (individual cell/ml culture fluid): pelotomaculum schinkii 5×107, pelotomaculumpropionicicum 2.5 × 107, syntrophobacter wolinii 3×106, methanospirillum hungatei 1.4 × 107, methanoculleuspalmolei 1.4×107, methanoculleus bourgensis 1.4 × 107, methanosarcina barkeri 2.5×107, methanosarcina mazei 3 × 107.
Sealing, 20 DEG C of static gas wave refrigerator, obtain hydrolysis propanoic acid methane producing composite bacterium group.The flora of acquisition is inoculated in ph respectively In the fresh bcty culture medium of 6.0, ph 7.0, ph 8.0, ph 9.0, inoculum concentration 20% (v/v), propanoic acid is final concentration of 3000mg/l, monitoring propionate concentration and methane production, result is shown in Fig. 2.
The metabolic capacity to sodium propionate for embodiment 3 composite flora
Prepare the bcty culture fluid of anaerobism, add the sodium propionate of final concentration of 10000mg/l, subpackage 150ml detests in 250ml In oxygen bottle, inoculate the wastewater anaerobic digestive device water outlet of 1% (v/v) dextran, sealing, 55 DEG C of static gas wave refrigerator.Treat propionic acid degradation rate Reach 50%, methane content is 30% about, according to below scheme interpolation function stem (individual cell/ml culture fluid): pelotomaculum schinkii 5×107, pelotomaculumpropionicicum 2.5 × 107, syntrophobacter wolinii 3×106, methanospirillum hungatei 1.4 × 107, methanoculleuspalmolei 1.4×107, methanoculleus bourgensis 1.4 × 107, methanosarcina barkeri 2.5×107, methanosarcina mazei 3 × 107.
Sealing, 55 DEG C of static gas wave refrigerator, obtain hydrolysis propanoic acid methane producing composite bacterium group.The flora of acquisition is inoculated in ph 7.0 Fresh bcty culture medium in, inoculum concentration 1% (v/v), propanoic acid final concentration is respectively 1g/l, 3g/l, 5g/l, 7g/l, 10g/l, 15g/l, 20g/l, 30g/l, sealing, 40 DEG C of static gas wave refrigerator, the propanoic acid relative amount in monitoring culture fluid, result is shown in Fig. 3.
The initial propionate concentration in propionate concentration (mg/l)/culture fluid in fermentation liquid during propanoic acid relative amount=monitoring (mg/l) × 100%.
Embodiment 4
Prepare the bcty culture fluid of anaerobism, add the sodium propionate of final concentration of 3000g/l, subpackage 300ml is in 550ml anaerobism In bottle, add 10% (w/v) curable type carrier glass sand, matched group not reinforcement type carrier, 121 DEG C of sterilizing 30min in anaerobism bottle, connect Plant 10% (v/v) embodiment 2 gained compound bacteria, 10%nahco3Adjust ph to 7.0-8.5, sealing, 40 DEG C of static gas wave refrigerator.Detection third Acid concentration and methane production, after adding curable type carrier glass sand, the propanoic acid degradable time shortens 16 days, and degradation rate improves 62%, meanwhile, methane production brings up to 67% from 52%, and methane production improves 28.8% (Fig. 4 and Fig. 5).
Embodiment 5
Prepare the bcty culture fluid of anaerobism, add the sodium propionate of final concentration of 3000mg/l, subpackage 300ml detests in 600ml In oxygen bottle, add in anaerobism bottle 10% (w/v) curable type carrier hollow glass micropearl cl, hollow glass micropearl n60, biological magnet ring, Activated carbon felt, activated carbon, matched group not reinforcement type carrier, 121 DEG C of sterilizing 30min, inoculation 10% (v/v) embodiment 2 gained is multiple Close bacterium, with 10%nahco3Adjust ph to 7.0-8.5, sealing, 40 DEG C of static gas wave refrigerator, continuous monitoring propionate concentration and methane production.
As shown in fig. 6, curable type carrier of the present invention can significantly promote propanoic acid syntrophism metabolic process.Activated carbon has aobvious Write the effect accelerating propanoic acid syntrophism metabolism to start, under the conditions of this group, only reach the most degradation rate to propanoic acid with 17 days (93.63%), it is 3.8 times of concurrent control group propionic acid degradation rate (25.43%)；Hollow glass micropearl cl and hollow glass micropearl Cl+n60 group completed the degradable of propanoic acid at the 19th day, was 2.28 times of concurrent control group propionic acid degradation rate (43.8%)； When reaction proceeds to 21 days, the propionic acid degradation rate of biological magnet ring, activated carbon felt and matched group is respectively 94.86%, 88.09% With 78.05%, two groups of curable type carriers all can effectively accelerate propanoic acid syntrophism to aoxidize.Wherein, the particle size range of hollow glass micropearl cl For 200-250 μm (similarly hereinafter)；The particle size range of hollow glass micropearl n60 is 50-60 μm (similarly hereinafter).
Embodiment 6
After the completion of propanoic acid anaerobic digester system starts, continuously add 3000ppm sodium propionate.As shown in fig. 7, curable type carrier To the operational efficiency of propanoic acid metabolic conversion methane, there is significant facilitation.
Embodiment 7
Prepare the bcty culture fluid of anaerobism, add the sodium propionate of final concentration of 3000mg/l, subpackage 50ml is in 120ml anaerobism In bottle, in anaerobism bottle, it is separately added into the curable type carrier hollow glass micropearl cl of 0%, 10%, 20%, 30%, 40% (w/v), 121 DEG C sterilizing 30min, inoculate 10% (v/v) embodiment 2 gained compound bacteria, 10%nahco3Adjust ph to 7.0-8.0, seal, 40 DEG C, Static gas wave refrigerator, continuous monitoring propionate concentration and methane production, result is as shown in figure 8, curable type carrier can accelerate the syntrophism oxygen of propanoic acid Change methanogenic speed, the impact to propanoic acid metabolic rate for the curable type carrier of different content simultaneously has different.Propanoic acid starts Stage ch4Yield is c20 > c30 >c40,c10> ck, propionic acid degradation rate isC20=c30=c40>c10>ck；Propanoic acid syntrophism metabolism After the completion of startup, the propanoic acid that second adds 3000mg/l, then propionic acid degradation rate c20 > c10 > c30 > c40, methane production c20 > c10>c30>c40.Wherein, c10, c20, c30 and c40 represent addition respectively is 10% (w/v), 20% (w/v), 30% (w/ The curable type carrier hollow glass micropearl cl of v) He 40% (w/v), ck represent without curable type carrier.
Propanoic acid syntrophism metabolic process is easily affected by hydrogen dividing potential drop in anaerobic digester system, and too high hydrogen dividing potential drop easily causes to detest The accumulation of organic acid such as acetic acid, propanoic acid and butanoic acid in oxygen digestive system, thus leading to system to be acidified, operational efficiency reduces, aerogenesis Reduce.As shown in figure 9, when by the use of hollow glass micropearl cl (c20) as curable type carrier, the inventive method can effectively facilitate detests H in oxygen digestive system2Transhipment metabolism, make system maintain relatively low hydrogen dividing potential drop state.Therefore so that anaerobic digester system more Plus stable, degeneration-resistant row is higher so as to operating load is significantly lifted.

Claims (7)

1. a kind of promote anaeroic digestor to start and accelerate the method that propionic acid is methane it is characterised in that methods described bag Include following steps:
(1) preparation of compound bacteria:
1) with dextran wastewater anaerobic digestive device water outlet as initial inoculum, using sodium propionate as carbon source, carry out inoculated and cultured To culture, propionic acid degradation rate reaches 50-80%, and methane content is 30-50%；
2) by step 1) gains transfer to fresh culture culture, and press every ml step 1) and culture fluid, the following antibacterial of addition:
3) by step 2) gains carry out sealing culture, obtain final product the compound bacteria of described Rapid reversal propionic acid accumulation；
(2) prepare fresh culture, add the sodium propionate of final concentration of 3000mg/l, inoculate 10% step (1) gained compound bacteria, 40 DEG C of quiescent culture reach 50-80% to propionic acid degradation rate, and methane content is 30-50%；
(3) described for step (2) culture is transferred to fresh culture, adds curable type to carry with the ratio of mass percent 1-20% Body, the hollow glass micropearl that described curable type carrier includes the biocycle for 6-15mm for the internal diameter, particle diameter is 50-250 μm, particle diameter are 1- The activated carbon of 5mm, the solid pv ball of a diameter of 5-12mm, bulk density are 0.1-0.2g/cm3One of activated carbon felt；
(4) by step (3) gains sealing simultaneously quiescent culture, until propionic acid degradation rate reaches 70-100%, as complete propanoic acid and detest The start-up course of oxygen digestive system；Propanoic acid anaerobic digester system continues to add the sodium propionate of 1-30g/l after the completion of starting, and 40 DEG C quiet Put culture, running after as propanoic acid anaerobic digester system starts.
2. method according to claim 1 it is characterised in that it is characterized in that, in step 1) inoculation when, inoculum concentration be 1- 20%, condition of culture is sealing culture under 25-55 DEG C, ph=6.0-9.0.
3. method according to claim 1 is it is characterised in that step 1) in, the addition of sodium propionate is 1500- 10000mg/l.
4. method according to claim 1 is it is characterised in that described culture medium, based on every liter of culture medium, including 1g nh4Cl, 1g yeast powder, 1g tryptone, 0.5g cysteine hydrochloride, 50ml a great number of elements solution, 10ml trace element is molten Liquid, 10ml Thioctic Acid 1,10mg "diazoresorcinol"；Described every liter of inclusion 6g kh of a great number of elements2po4, 12g nacl, 2g mgcl2· 6h2O, 1.6g cacl2·2h2o；Described every liter of inclusion 12.8g aminotriacetic acid of trace element solution, 1.35g fecl3· 6h2O, 0.1g mncl2·4h2O, 0.1g cocl2·6h2O, 0.1g zncl2, 0.025g cucl2·2h2O, 0.01g h3bo3, 0.024g na2moo4·2h2O, 1g nacl, 0.12g nicl2·6h2O, 0.026g na2seo3·5h2o.
5. method according to claim 1 is it is characterised in that step 3) in condition of culture be: cultivation temperature is 25-55 DEG C, ph is 6.0-9.0.
6. method according to claim 1 it is characterised in that described curable type carrier for particle diameter be 1-5mm activated carbon or Particle diameter is 50-250 μm of hollow glass micropearl.
7. method according to claim 1 is it is characterised in that described curable type carrier is the activated carbon of 1-5mm for particle diameter.
CN201610727806.XA 2016-08-25 2016-08-25 Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane Pending CN106350567A (en)

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CN112342250A (en) * 2019-08-07 2021-02-09 首都师范大学 Method for preparing granular sludge for producing methane by degrading propionic acid

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