CN106323945A - Solid-phase-extraction-column-integrated surface-enhanced Raman detection chip - Google Patents
Solid-phase-extraction-column-integrated surface-enhanced Raman detection chip Download PDFInfo
- Publication number
- CN106323945A CN106323945A CN201610998040.9A CN201610998040A CN106323945A CN 106323945 A CN106323945 A CN 106323945A CN 201610998040 A CN201610998040 A CN 201610998040A CN 106323945 A CN106323945 A CN 106323945A
- Authority
- CN
- China
- Prior art keywords
- substrate
- sample
- column
- detection
- chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Abstract
The invention discloses a solid-phase-extraction-column-integrated surface-enhanced Raman detection chip which comprises a chip body, wherein the chip body comprises a lower substrate, a middle substrate and an upper substrate; the middle substrate is arranged between the lower substrate and the upper substrate; the upper substrate comprises a sample inlet, a sample outlet, a particle introduction channel inlet, a packed column bed channel and a dam structure; the middle substrate comprises a sample detection tank, a sample outlet, a sieve plate tank, flow microchannels and a sample inlet; and the lower substrate comprises a second hard substrate, a second adhesive layer, a metal covering film, metal columns, flow microchannels, a first adhesive layer and a first hard substrate. The metal column bundle with three-dimensional gain cylindrical surfaces is arranged in the detection area of the detection chip, thereby greatly increasing the contact area between the detection area and detected molecules and greatly enhancing the gain factor and detection sensitivity. The particle introduction channel can implement particle filling and renewal. The detection chip is simple in structure and convenient to use.
Description
Technical field
The present invention relates to detection chip technical field, the surface-enhanced Raman detection of a kind of integrated solid-phase extraction column
Chip.
Background technology
Solid-Phase Extraction (SPE) technology is widely used in separation/purification, concentrates or enriched with trace determinand, removing chaff interference,
Improve concentration and the detection sensitivity of determinand, be a kind of conventional and important sample pretreating method.Utilize chip micro Process
The microchannel structure that technology is formed completes SPE operation on microfluidic platform to be had less than many conventional SPE technology
Advantage, such as rate of extraction are fast, and sample/reagent consumes low, can be integrated etc. in other operating units.
Surface enhanced Raman technique (Surface Enhanced Raman Scattering, SERS) refers to by slightly
Rough metal surface or metal Nano structure strengthen the technology of the Raman signal of binding molecule, and it can make Raman signal strengthen
1010～1011 times, it means that it can detect individual molecule, owing to it has the sensitivity of superelevation, ERS technology is by extensively
It is applied to the fields such as chemistry, biology, medical science.
Existing detection chip also exist due to the detection zone of test chip arrange for nano-pillar group, cause detection chip
Gain coefficient and detection sensitivity poor；The microgranule of detection chip is filled and updates inconvenience；Detection chip can not preferably by
Solid phase extraction techniques and surface enhanced Raman technique combine so that detection sensitivity and the speed of detection chip still need to raising etc.
Problem.
Summary of the invention
It is an object of the invention to provide the surface-enhanced Raman detection chip of a kind of integrated solid-phase extraction column, possess detection
The advantage that sensitivity is increased, solves the problem that detection chip detection sensitivity is poor.
For achieving the above object, the present invention provides following technical scheme: the surface enhanced of a kind of integrated solid-phase extraction column draws
Graceful detection chip, including chip body, described chip body includes underlying basal, middle level substrate and upper substrate, underlying basal
And between upper substrate, it being set to middle level substrate, upper substrate includes injection port, outlet, microgranule introduction passage entrance, filling
Post bed passage and dam structure, middle level substrate includes sample detection pond, sample export, sieve plate groove, microchannel and sample inlet, under
Layer substrate includes that the second hard substrate, the second tack coat, metal covering film, metal column, fluid channel, the first tack coat and first are hard
Matter substrate.
Preferably, the two ends of described upper substrate are respectively set to injection port and outlet, and the bottom of upper substrate is arranged
Being provided with dam structure, dam number of structures is 2, is provided with packed column bed passage between dam structure, and the side of dam structure is provided with
Microgranule introduction passage entrance.
Preferably, one end of described middle level substrate is provided with sample detection pond, and the side in sample detection pond is provided with miniflow
Passage, the two ends of microchannel are respectively arranged with sample export and sample inlet, the miniflow between sample export and sample export
Being provided with sieve plate groove on passage, the quantity of sieve plate groove is 2.
Preferably, the top of described underlying basal is provided with the second hard substrate, is provided with the first hard substrate bottom it,
It is provided with some metal columns between second hard substrate and the first hard substrate, between metal column, is formed with fluid channel.
Preferably, between described second hard substrate and metal column, it is provided with the second tack coat and metal covering film, first
The first tack coat it is provided with between hard substrate and metal column.
Compared with prior art, beneficial effects of the present invention is as follows:
1, the present invention has the metal column group of three-dimensional gain cylinder by being arranged on the detection zone setting of test chip, is greatly increased
Detection zone and the contact area of testing molecule, greatly improve gain coefficient and detection sensitivity.
2, the present invention is by being arranged on the microgranule introduction passage of upper substrate, can realize filling and the renewal of microgranule, it is simple to
Carry out Solid-Phase Extraction, simple in construction, easy to use, concentrate or enriched with trace determinand, remove chaff interference, improving the dense of determinand
Degree, is effectively increased the accuracy of detection of detection chip.
3, the present invention is by the upper substrate arranged and the cooperation of underlying basal, it is achieved that solid phase extraction techniques and surface increase
Matching of strong Raman technology, simple in construction, improve detection speed and the detection sensitivity of detection chip, easy to use.
Accompanying drawing explanation
Fig. 1 is present configuration schematic diagram；
Fig. 2 is present configuration upper substrate schematic diagram；
Fig. 3 is present configuration middle level substrate schematic diagram；
Fig. 4 is present configuration underlying basal schematic diagram.
In figure: 1-chip body, 2-underlying basal, 21-the second hard substrate, 22-the second tack coat, 23-metal covers
Film, 24-metal column, 25-fluid channel, 26-the first tack coat, 27-the first hard substrate, 3-middle level substrate, 31-sample detection
Pond, 32-sample export, 33-sieve plate groove, 34-microchannel, 35-sample inlet, 4-upper substrate, 5-injection port, 6-goes out sample
Mouthful, 7-microgranule introduction passage entrance, 8-packed column bed passage, 9-dam structure.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.Based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under not making creative work premise
Embodiment, broadly falls into the scope of protection of the invention.
Referring to Fig. 1-4, the present invention provides a kind of technical scheme: the surface-enhanced Raman of a kind of integrated solid-phase extraction column is examined
Surveying chip, including chip body 1, described chip body 1 includes underlying basal 2, middle level substrate 3 and upper substrate 4, underlying basal
Being set to middle level substrate 3 between 2 and upper substrate 4, upper substrate 4 includes injection port 5, outlet 6, microgranule introduction passage entrance
7, packed column bed passage 8 and dam structure 9, middle level substrate 3 includes that sample detection pond 31, sample export 32, sieve plate groove 33, miniflow lead to
Road 34 and sample inlet 35, underlying basal 2 includes second hard substrate the 21, second tack coat 22, metal covering film 23, metal column
24, fluid channel the 25, first tack coat 26 and the first hard substrate 27.
The two ends of described upper substrate 4 are respectively set to injection port 5 and outlet 6, the bottom setting of upper substrate 4
Having dam structure 9, dam structure 9 quantity is 2, is provided with packed column bed passage 8 between dam structure 9, and the side of dam structure 9 is provided with
Microgranule introduction passage entrance 7, microgranule introduction passage, filling and the renewal of microgranule can be realized, it is simple to carry out Solid-Phase Extraction, structure letter
Single, easy to use, it is effectively increased the accuracy of detection of detection chip, one end of described middle level substrate 3 is provided with sample detection pond
31, the side in sample detection pond 31 is provided with microchannel 34, and the two ends of microchannel 34 are respectively arranged with sample export 32 He
Sample inlet 35, the microchannel 34 between sample export 32 and sample export 32 is provided with sieve plate groove 33, sieve plate groove 33
Quantity is 2, improves the speed of detection, and the top of described underlying basal 2 is provided with the second hard substrate 21, is provided with bottom it
First hard substrate 27, is provided with some metal columns 24, metal column between the second hard substrate 21 and the first hard substrate 27
It is formed with fluid channel 25 between 24, between described second hard substrate 21 and metal column 24, is provided with the second tack coat 22 and metal
Coverlay 23, is provided with the first tack coat 26, considerably increases detection zone and treat between the first hard substrate 27 and metal column 24
Survey the contact area of molecule, greatly improve gain coefficient and detection sensitivity.
Operation principle: the surface-enhanced Raman detection chip of this kind of integrated solid-phase extraction column, microgranule is micro-by upper substrate 4
Grain introduction passage entrance enters, and detection sample is entered into the sample inlet 35 of middle level substrate 3 by the injection port 5 of upper substrate 4
During carry out Solid-Phase Extraction, concentrate or enriched with trace determinand, remove chaff interference after entered miniflow by sample inlet 35 again and lead to
Road 34 enters sample detection pond 31 by sample export 32, has three-dimensional gain by arranging underlying basal 2 in detection zone setting
The metal column group of cylinder, makes detection zone contact with testing molecule and detects.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, permissible
Understand and these embodiments can be carried out multiple change without departing from the principles and spirit of the present invention, revise, replace
And modification, the scope of the present invention be defined by the appended.
Claims (5)
1. a surface-enhanced Raman detection chip for integrated solid-phase extraction column, including chip body (1), it is characterised in that: institute
State chip body (1) and include underlying basal (2), middle level substrate (3) and upper substrate (4), underlying basal (2) and upper substrate
(4) being set to middle level substrate (3) between, upper substrate (4) includes injection port (5), outlet (6), microgranule introduction passage entrance
(7), packed column bed passage (8) and dam structure (9), middle level substrate (3) includes sample detection pond (31), sample export (32), sieve
Board slot (33), microchannel (34) and sample inlet (35), underlying basal (2) includes the second hard substrate (21), the second bonding
Layer (22), metal covering film (23), metal column (24), fluid channel (25), the first tack coat (26) and the first hard substrate (27).
The surface-enhanced Raman detection chip of a kind of integrated solid-phase extraction column the most according to claim 1, it is characterised in that:
The two ends of described upper substrate (4) are respectively set to injection port (5) and outlet (6), the bottom setting of upper substrate (4)
Having dam structure (9), dam structure (9) quantity is 2, is provided with packed column bed passage (8) between dam structure (9), dam structure (9)
Side is provided with microgranule introduction passage entrance (7).
The surface-enhanced Raman detection chip of a kind of integrated solid-phase extraction column the most according to claim 1, it is characterised in that:
The one end in described middle level substrate (3) is provided with sample detection pond (31), and the side of sample detection pond (31) is provided with microchannel
(34), the two ends of microchannel (34) are respectively arranged with sample export (32) and sample inlet (35), sample export (32) and sample
Being provided with sieve plate groove (33) on microchannel (34) between product outlet (32), the quantity of sieve plate groove (33) is 2.
The surface-enhanced Raman detection chip of a kind of integrated solid-phase extraction column the most according to claim 1, it is characterised in that:
The top of described underlying basal (2) is provided with the second hard substrate (21), is provided with the first hard substrate (27) bottom it, and second
Being provided with some metal columns (24) between hard substrate (21) and the first hard substrate (27), metal column is formed between (24)
Fluid channel (25).
The surface-enhanced Raman detection chip of a kind of integrated solid-phase extraction column the most according to claim 1, it is characterised in that:
The second tack coat (22) and metal covering film (23) it is provided with between described second hard substrate (21) and metal column (24), first
The first tack coat (26) it is provided with between hard substrate (27) and metal column (24).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610998040.9A CN106323945A (en) | 2016-11-14 | 2016-11-14 | Solid-phase-extraction-column-integrated surface-enhanced Raman detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610998040.9A CN106323945A (en) | 2016-11-14 | 2016-11-14 | Solid-phase-extraction-column-integrated surface-enhanced Raman detection chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106323945A true CN106323945A (en) | 2017-01-11 |
Family
ID=57816455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610998040.9A Pending CN106323945A (en) | 2016-11-14 | 2016-11-14 | Solid-phase-extraction-column-integrated surface-enhanced Raman detection chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106323945A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107036874A (en) * | 2017-05-25 | 2017-08-11 | 华东理工大学 | The encapsulation type enrichment and separation device detected for online solid phase spectra |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101169403A (en) * | 2006-10-25 | 2008-04-30 | 中国科学院大连化学物理研究所 | Micro-fluidic chip and its preparation and uses |
| US20080160630A1 (en) * | 2006-12-29 | 2008-07-03 | Intel Corporation | Enzymatic signal generation and detection of binding complexes in stationary fluidic chip |
| WO2012058515A2 (en) * | 2010-10-29 | 2012-05-03 | Thermo Finnigan Llc | Modular multiple-column chromatography cartridge |
| CN103026298A (en) * | 2010-05-21 | 2013-04-03 | 普林斯顿大学 | Structures for enhancement of local electric field, light absorption, light radiation, material detection and methods for making and using of the same |
| CN103954605A (en) * | 2014-04-30 | 2014-07-30 | 中国科学院物理研究所 | Micro-flow detector based on SERS (Surface Enhanced Raman Scattering) principle and preparation method thereof |
| CN203929645U (en) * | 2014-05-07 | 2014-11-05 | 中国科学院物理研究所 | Micro-fluidic surface-enhanced Raman test chip |
| CN104792767A (en) * | 2015-05-08 | 2015-07-22 | 厦门大学 | Microfluidic chip for SERS (surface-enhanced Raman scattering) detection by sol method and using method of microfluidic chip |
| CN105466905A (en) * | 2015-10-27 | 2016-04-06 | 厦门市普识纳米科技有限公司 | Solid phase extraction column integrated surface enhanced Raman detection chip |
-
2016
- 2016-11-14 CN CN201610998040.9A patent/CN106323945A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101169403A (en) * | 2006-10-25 | 2008-04-30 | 中国科学院大连化学物理研究所 | Micro-fluidic chip and its preparation and uses |
| US20080160630A1 (en) * | 2006-12-29 | 2008-07-03 | Intel Corporation | Enzymatic signal generation and detection of binding complexes in stationary fluidic chip |
| CN103026298A (en) * | 2010-05-21 | 2013-04-03 | 普林斯顿大学 | Structures for enhancement of local electric field, light absorption, light radiation, material detection and methods for making and using of the same |
| WO2012058515A2 (en) * | 2010-10-29 | 2012-05-03 | Thermo Finnigan Llc | Modular multiple-column chromatography cartridge |
| CN103954605A (en) * | 2014-04-30 | 2014-07-30 | 中国科学院物理研究所 | Micro-flow detector based on SERS (Surface Enhanced Raman Scattering) principle and preparation method thereof |
| CN203929645U (en) * | 2014-05-07 | 2014-11-05 | 中国科学院物理研究所 | Micro-fluidic surface-enhanced Raman test chip |
| CN104792767A (en) * | 2015-05-08 | 2015-07-22 | 厦门大学 | Microfluidic chip for SERS (surface-enhanced Raman scattering) detection by sol method and using method of microfluidic chip |
| CN105466905A (en) * | 2015-10-27 | 2016-04-06 | 厦门市普识纳米科技有限公司 | Solid phase extraction column integrated surface enhanced Raman detection chip |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107036874A (en) * | 2017-05-25 | 2017-08-11 | 华东理工大学 | The encapsulation type enrichment and separation device detected for online solid phase spectra |
| CN107036874B (en) * | 2017-05-25 | 2021-03-16 | 华东理工大学 | A encapsulation type enrichment separator for online solid-phase spectral detection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hyun et al. | Microfluidic flow fractionation device for label-free isolation of circulating tumor cells (CTCs) from breast cancer patients | |
| CN107402295B (en) | Circulating tumor cell is automatically separated purifying micro-fluidic chip and its isolation and purification method | |
| Rana et al. | Advancements in microfluidic technologies for isolation and early detection of circulating cancer-related biomarkers | |
| CN103983794B (en) | A kind of micro-fluidic chip and a kind of microfluidic methods | |
| CN109070080B (en) | Particle separation device and particle separation method | |
| US20120177537A1 (en) | Apparatus and method for separating plasma | |
| WO2008011486A3 (en) | Detection or isolation of target molecules using a microchannel apparatus | |
| CN105466905B (en) | A kind of surface-enhanced Raman detection chip of integrated solid-phase extraction column | |
| CN104759302B (en) | flow cytometer | |
| CN105259164A (en) | Micro-fluidic chip for multi-object quantitative detection based on magnetic particle chemiluminescence | |
| CN103616427A (en) | Micro-fluid control electrochemical biological sensing system for simultaneous detection on different serum markers of prostate cancer | |
| CN104165906B (en) | A kind of Viral diagnosis instrument | |
| CN107400623B (en) | Micro-fluidic chip for automatically capturing circulating tumor cells and automatic capturing method thereof | |
| CN204637683U (en) | A kind of slipstream filter post purification devices of multicolumn parallel connection | |
| CN106323945A (en) | Solid-phase-extraction-column-integrated surface-enhanced Raman detection chip | |
| CN105424784A (en) | Microfluidic chip for detecting heavy metal ions in water and detection method | |
| CN107209198A (en) | Target component is positioned and discharged | |
| WO2016019401A1 (en) | Microfluidic device with smooth surface for enrichment of rare cells and biomarkers from a biological fluid | |
| US20140087414A1 (en) | Magnetophoretic analyte selection and concentration | |
| CN206666547U (en) | A kind of biochip for being used to screen rare cell in positioning and detection blood | |
| CN104458973A (en) | Online determination method suitable for zearalenone and metabolites of zearalenone | |
| CN103131693B (en) | Separation method of rare cells in blood | |
| CN207520642U (en) | It is a kind of to match liquid layer analysis system online | |
| CN209619323U (en) | A kind of 384 hole array micro-fluidic chips | |
| CN207143246U (en) | A kind of integrated form Terahertz superstructure nano biological sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |