CN106318852B - Oscillating jet microbubble bioreactor - Google Patents
Oscillating jet microbubble bioreactor Download PDFInfo
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- CN106318852B CN106318852B CN201510349664.3A CN201510349664A CN106318852B CN 106318852 B CN106318852 B CN 106318852B CN 201510349664 A CN201510349664 A CN 201510349664A CN 106318852 B CN106318852 B CN 106318852B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
Abstract
The present invention provides a kind of oscillating jet microbubble bioreactor, including reactor body, gas distributor, culture solution external circulation line, air supply pipe, oscillating jet mixer and air inlet pipeline.By adding oscillating jet mixer, culture solution and gas are first introduced into the oscillating jet mixer and are sufficiently mixed, a large amount of microbubbles are generated under oscillating jet effect using gas distributor, since microbubble can significantly improve gas liquid interfacial area under same air inflow, to play the important function for strengthening alternate transmittance process, the purpose of efficient carbon sequestration and microdisk electrode is reached；Oscillating jet mixer of the invention compares common normal pressure air inlet, and gas supply energy consumption can be significantly reduced.
Description
Technical field
The present invention relates to algae culture and CO2Fixed technical field of bioengineering, more particularly to a kind of efficiently mixing
Oscillating jet microbubble bioreactor.
Background technique
In field of microalgae cultivation, it is contemplated that microalgae is more sensitive to mechanical stirring, generally selects gas lift or bubble type photoproduction
Object reactor, although both bioreactors avoid mechanical stirring to alga cells using the method for gas-liquid cycle mixing
Injury, but the problem lower there are still gas-liquid mixed, mass transfer, heat transfer efficiency, to strengthen gas-liquid mixed and alternate transmitting
Process, technical staff have made various improvement, and such as application No. is 200420009076.2, entitled " pneumatic lifting photobiological reacts
In the patent of invention of device ", the nozzle quantity and shape of gas distributor are improved, to be more conducive to gas in culture solution
Dispersion, eliminate dead angle.But in practical application, no matter how the structure of gas distributor is designed, gas-liquid mixed efficiency
Improvement is not obvious, and is primarily due to gas and liquid only carries out a gas-liquid mixed in reactor, mass transfer area is by bubble
Size limitation, to influence mass-transfer efficiency.Chinese Patent Application No. uses water circulating pump by culture solution for 200910062087.4
It draws outer mix using Venturi tube with carbon dioxide gas of reactor tank to be sent into reactor tank, this uses Venturi tube contraction section
By in gas injection such as pipeline, higher carbon dioxide conversion is achieved.But Venturi tube injection described in the patent is mixed
Conjunction is actually identical as conventional jet pump, and Venturi tube still falls within normal pressure gas supply, without any change in terms of energy consumption.
The diffusion action that gas-liquid premixer utilizes fluid wall attachment effect and diffuser pipe is vibrated, any moving component is not necessarily to,
The oscillating jet of high frequency can be generated, there is many advantages, such as easily fabricated, high reliablity, Maintenance free.This oscillation gas
Liquid premixer can be applied to the reactor of various gas lifts, can produce 20 in a liquid using specific gas distributor
~100 μm microbubble (Zimmerman, W.B., Zandi, M., Bandulasena, H.C.H., Tesar, V., Gilmour,
D.J.,Ying,K.,2011.Design of an airlift bioreactor and pilot scale studies
with fluidic oscillator induced microbubbles for growth of a microalgae
Dunaliella salina.Applied Energy 88,335.)。
The technical thought of present invention combination oscillating jet, independent research oscillating jet mixer, structure novel, manufacture letter
It is single, it is at low cost, microbubble effectively can be generated in microalgae photoproduction reaction device, be mixed in conjunction with oscillation, play reinforcing gas-liquid mixed
And mass transport process, carbon sequestration energy consumption is reduced, the economy of pilot scale culture microalgae is effectively improved, to solve mesh to a certain extent
The disadvantages of generally existing gas-liquid mixed is uneven in preceding photoproduction reaction device, and mass-transfer efficiency is low, and gas supply energy consumption is higher.
Summary of the invention
In view of the foregoing deficiencies of prior art, the object of the present invention is to provide a kind of structures to be simple and convenient to operate, saves
The about microbubble bioreactor of energy consumption, efficient carbon sequestration and algae culture.By combining injection principle and oscillating jet technology
The more efficient air and liquid mixer of one kind is provided to be pre-mixed to gas and liquid.By the high frequency vibrating of oscillating jet mixer
Bubble is swung to generate contraction at gas distribution holes and accelerate break-off that can greatly improve gas-liquid to generate microbubble
Contact surface promotes gas-liquid mass transfer and photosynthesis.
In order to achieve the above objects and other related objects, the present invention provides a kind of oscillating jet microbubble optical-biological reaction
Device, the oscillating jet microbubble bioreactor include: reactor body, gas distributor, culture solution external circulation line,
Air supply pipe, oscillating jet mixer and air inlet pipeline；
The oscillating jet mixer includes: algae solution jet pipe, gas suction chamber, trunnion, air inlet cavity, feedback channel, vibration
Swing chamber, diffuser pipe, feedback flow pipeline and three-way connection；
The outlet of algae solution jet pipe is via in the entrance insertion gas suction chamber of gas suction chamber；The outlet of gas suction chamber with
The entrance of trunnion is connected, and the outlet of trunnion is connected with the entrance of diffuser pipe；The air inlet cavity is located at gas sucking
The side of chamber, and the outlet of air inlet cavity is connected with the gas suction chamber；Feedback channel is located at the two sides of the trunnion, and
The outlet of feedback channel is connected with the outlet of trunnion；The oscillation chamber is connected with the entrance of diffuser pipe；The feedback flow tube
The one end on road is connected via the three-way connection with the outlet of diffuser pipe, and the other end is connected with the entrance of feedback channel；Institute
It states algae solution jet pipe, gas suction chamber, trunnion, feedback channel, oscillation chamber and diffuser pipe and forms axisymmetric cavity structure；
The gas distributor is located at the inside of the reactor body, and is located at the bottom of the reactor body；Institute
The one end for stating culture solution external circulation line is connected via the side wall of the reactor body with the inside of the reactor body,
The other end is connected with the entrance of algae solution jet pipe；The air supply pipe is connected with the entrance of air inlet cavity；The air inlet pipeline one
End is connected via the three-way connection with the outlet of the diffuser pipe, and the other end is connected with the gas distributor.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the feedback channel, institute
The quantity for stating diffuser pipe and the feedback flow pipeline is two, and the oscillation chamber is between described two diffuser pipes.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the algae solution jet pipe enters
Mouthful width and the ratio between the width of the trunnion be 1~5, the width of the width of the outlet of the algae solution jet pipe and the trunnion it
Than being 0.2~0.8, the ratio between the length of the algae solution jet pipe and the width of the trunnion are 2~10.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the gas suction chamber
Side wall is inclined surface, and the angle that opposite two sidewalls are formed is 10 °~60 °；The outlet and the algae solution of the gas suction chamber
The outlet of jet pipe is in the same plane.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the width of the trunnion is big
In the size of microalgae, the ratio between the length of the trunnion and width are 2~10.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the air inlet cavity goes out
Mouth is located between the entrance and outlet of the algae solution jet pipe；The entrance of the air inlet cavity is circle, the air inlet cavity entrance
Diameter and the ratio between the width of the trunnion be 1~5, the width of the outlet of the air inlet cavity and the ratio between the width of the trunnion
It is 0.5~5.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the entrance of the feedback pipe
Width and the ratio between the width of the trunnion be 2~10, the width of the outlet of the feedback pipe and the ratio between the width of the trunnion
It is 0.1~2.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the oscillation chamber includes position
Flat sidewall in two sides and the circular groove bottom that is connected with the flat sidewall；The flat sidewall positioned at two sides
Institute is extended to from the junction of the entrance of the outlet of the trunnion, the outlet of described two feedback channels and described two diffuser pipes
It states in diffuser pipe；The flat sidewall positioned at two sides forms 20 °~90 ° of angle.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the oscillation chamber is axis pair
Claim structure, the outlet face of the circular groove bottom and the trunnion；The circular groove bottom to the trunnion goes out
The ratio between the distance of mouth and the width of the trunnion are 5~15；The arc length of the circular groove bottom and the trunnion
The ratio between width is 1~5.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the diffuser pipe includes outer
Side wall and inner sidewall, symmetry axis of the inner sidewall close to the axisymmetric cavity structure；The inner sidewall includes being connected with each other
First part and second part, entrance of the first part close to the diffuser pipe, and, institute parallel with the lateral wall
Second part is stated close to the outlet of the diffuser pipe, and forms 5 °~20 ° of angle with the lateral wall；The diffuser pipe enters
Mouthful width and the ratio between the width of the trunnion be 2~10, the width of the width of the outlet of the diffuser pipe and the trunnion it
Than being 2~10.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the algae solution jet pipe, gas
Body suction chamber, trunnion, air inlet cavity, feedback channel, oscillation chamber, diffuser pipe constitute the cavity of the oscillating jet mixer, institute
Stating the ratio between the height of cavity and the width of the trunnion is 0.5~10.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the micro- gas of oscillating jet
Steep bioreactor further include: culture solution import, culture solution outlet, exhaust outlet, inner light source pipe and water circulating pump；
The culture solution import and the exhaust outlet are located at the top of the reactor body；The culture solution outlet is located at
The bottom of the reactor body；The inner light source pipe is inserted perpendicularly into the reactor body from the top of the reactor body
Inside；The water circulating pump is connected to the culture solution outer circulating tube road.
As a kind of preferred embodiment of oscillating jet microbubble bioreactor of the invention, the reactor body and
The inner light source pipe is cylinder, and the diameter ratio of the diameter of the inner light source pipe and the reactor body is 5~20.
As described above, oscillating jet microbubble bioreactor of the invention, has the advantages that the present invention is logical
It crosses and adds oscillating jet mixer, culture solution and gas are first introduced into the oscillating jet mixer and are sufficiently mixed, using
Gas distributor generates a large amount of microbubbles under oscillating jet effect, since microbubble can significantly improve under same air inflow
Gas liquid interfacial area reaches the purpose of efficient carbon sequestration and microdisk electrode to play the important function for strengthening alternate transmittance process；
Oscillating jet mixer of the invention compares common normal pressure air inlet, and gas supply energy consumption can be significantly reduced.
Detailed description of the invention
Fig. 1 is shown as the structural schematic diagram of oscillating jet microbubble bioreactor of the invention.
The structure for the oscillating jet mixer that Fig. 2 is shown as in oscillating jet microbubble bioreactor of the invention is shown
It is intended to.
Fig. 3 is shown as an expansion of the oscillating jet mixer in oscillating jet microbubble bioreactor of the invention
The pressure changing schematic diagram of pressure pipe outlet.
The oscillating jet mixer that Fig. 4 to Fig. 7 is shown as in oscillating jet microbubble bioreactor of the invention exists
The situation of change schematic diagram of one cycle of oscillation flow field streamline.
Component label instructions
1 culture solution import
The outlet of 2 culture solutions
3 air supply pipes
4 exhaust outlets
5 reactor bodies
6 culture liquid zones
7 gas Disengagement zone
8 inner light source pipes
9 gas distributors
10 culture solution external circulation lines
11 water circulating pumps
12 oscillating jet mixers
121 algae solution jet pipes
122 gas suction chambers
123 trunnions
124 air inlet cavities
125 feedback channels
126 oscillation chambers
127 diffuser pipes
13 feedback flow pipelines
14 air inlet pipelines
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification
Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from
Various modifications or alterations are carried out under spirit of the invention.
Please refer to FIG. 1 to FIG. 7.It should be noted that diagram provided in the present embodiment only illustrates this in a schematic way
The basic conception of invention, though only show in diagram with related component in the present invention rather than package count when according to actual implementation
Mesh, shape and size are drawn, when actual implementation kenel, quantity and the ratio of each component can arbitrarily change for one kind, and its
Assembly layout kenel may also be increasingly complex.
Fig. 1 to 2 is please referred to, the present invention provides a kind of oscillating jet microbubble bioreactor, and the oscillating jet is micro-
Bubble bioreactor includes at least: reactor body 5, gas distributor 9, culture solution external circulation line 10, air supply pipe 3,
Oscillating jet mixer 12 and air inlet pipeline 14；The oscillating jet mixer 12 includes: algae solution jet pipe 121, gas suction chamber
122, trunnion 123, air inlet cavity 124, feedback channel 125, oscillation chamber 126, diffuser pipe 127, feedback flow pipeline 13 and three connect
Head；The outlet of algae solution jet pipe 121 is via in the entrance insertion gas suction chamber 122 of gas suction chamber 122；Gas suction chamber 122
Outlet be connected with the entrance of trunnion 123, the outlet of trunnion 123 is connected with the entrance of diffuser pipe 127；The air inlet cavity
124 are located at the side of the gas suction chamber 122, and the outlet of air inlet cavity 124 is connected with the gas suction chamber 122；
The feedback channel 125 is located at the two sides of the trunnion 123, and the outlet of feedback channel 125 is connected with the outlet of trunnion 123
It is logical；The oscillation chamber 126 is connected with the entrance of diffuser pipe 127；One end of the feedback flow pipeline 13 is connected via described three
Head is connected with the outlet of diffuser pipe 127, and the other end is connected with the entrance of feedback channel 125；The algae solution jet pipe 121, gas
Body suction chamber 122, trunnion 123, feedback channel 125, oscillation chamber 126 and diffuser pipe 127 form axisymmetric cavity structure；
The gas distributor 9 is located at the inside of the reactor body 5, and is located at the bottom of the reactor body 5；
One end of the culture solution external circulation line 10 is via the side wall of the reactor body 5 and the inside of the reactor body 5
It is connected, the other end is connected with the entrance of algae solution jet pipe 121；The air supply pipe 3 is connected with the entrance of air inlet cavity 124；
Described 14 one end of air inlet pipeline is connected via the three-way connection with the outlet of the diffuser pipe 127, the other end and the gas
Body distributor 9 is connected.
As an example, the quantity of the feedback channel 125, the diffuser pipe 127 and the feedback flow pipeline 13 is two
A, the oscillation chamber 126 is between described two diffuser pipes 127.
As an example, the ratio between width of the width of the entrance of the algae solution jet pipe 121 and the trunnion 123 is 1~5, institute
Stating the ratio between the width of the outlet of algae solution jet pipe 121 and the width of the trunnion 123 is 0.2~0.8, the algae solution jet pipe 121
The ratio between width of length and the trunnion 123 is 2~10.Preferably, in the present embodiment, the entrance of the algae solution jet pipe 121
The ratio between width of width and the trunnion 123 is 1.5~2.5, the width and the trunnion of the outlet of the algae solution jet pipe 121
The ratio between 123 width is 0.3~0.5, and the ratio between the length of the algae solution jet pipe 121 and the width of the trunnion 123 are 4~6.
As an example, the algae solution jet pipe 121 is inserted into after the gas suction chamber 122, the gas suction chamber 122
Real space is surrounded by the side wall of the algae solution jet pipe 121 and the gas suction chamber 122；The side of the gas suction chamber 122
Wall is two mutually angled clinoplains, and it is 10 °~60 ° that the side wall of the gas suction chamber 122, which is formed by angle,
Preferably, in the present embodiment, it is 30 °~45 ° that the side wall of the gas suction chamber 122, which is formed by angle,.
It should be noted that the length of the gas suction chamber 122 should within limits, to guarantee that the gas is inhaled
The outlet of the outlet and the algae solution jet pipe 121 that enter chamber 122 is in the same plane.
As an example, the width of the trunnion 123 should be greater than the size of microalgae, to ensure that the microalgae can pass through institute
State trunnion 123；The ratio between the length of the trunnion 123 and width are 2~10, it is preferable that in the present embodiment, the trunnion 123
The ratio between length and width are 3~5.
As an example, the outlet of the air inlet cavity 124 is located between the entrance and outlet of the algae solution jet pipe 121；Institute
The shape for stating the entrance of air inlet cavity 124 can be designed according to actual needs, the shape of the entrance of the air inlet cavity 124
It can be round, polygon or irregular structure, it is preferable that in the present embodiment, the shape of the entrance of the air inlet cavity 124 is
It is round；The shape of the entrance of the air inlet cavity 124 be it is circular under the premise of, the diameter of the entrance of the air inlet cavity 124 with
The ratio between width of the trunnion 123 is 1~5, the width of the width of the outlet of the air inlet cavity 124 and the trunnion 123 it
Than being 0.5~5, it is preferable that in the present embodiment, the width of the diameter of the entrance of the air inlet cavity 124 and the trunnion 123 it
Than being 2~3, the ratio between width of the width of the outlet of the air inlet cavity 124 and the trunnion 123 is 0.8~1.2.
As an example, the ratio between the width and the width of the trunnion 123 of the entrance of the feedback pipe 125 are 2~10, it is described
Feeding back the ratio between the width of the outlet of pipe 125 and the width of the trunnion 123 is 0.1~2；Preferably, described anti-in the present embodiment
Presenting the ratio between the width of the entrance of pipe 125 and the width of the trunnion 123 is 2.5~3.5, the width of the outlet of the feedback pipe 125
The ratio between degree and the width of the trunnion 123 are 0.8~1.5.
As an example, the oscillation chamber 126 includes being located at the flat sidewall of two sides and being connected with the flat sidewall
Circular groove bottom；Outlet of the flat sidewall positioned at two sides from the trunnion 123, described two feedback channels 125
Outlet and the junctions of entrance of described two diffuser pipes 127 extend in the diffuser pipe 127, i.e., the described oscillation chamber 126
By the outlet of the trunnion 123, the outlet of described two feedback channels 125, the flat sidewall, described two diffuser pipes 127
Entrance and the circular groove bottom surround.The flat sidewall positioned at two sides forms 20 °~90 ° of angle, preferably
Ground, in the present embodiment, the flat sidewall positioned at two sides forms 40 °~60 ° of angle.
As an example, the oscillation chamber 126 is axially symmetric structure, the circular groove bottom and the trunnion 123
Export face；The distance of the circular groove bottom to the outlet of the trunnion 123 and the ratio between the width of the trunnion 123 are
5~15；The ratio between width of the arc length of the circular groove bottom and the trunnion 123 is 1~5；Preferably, the circle
Arc-shaped groove bottom to the ratio between the distance of the outlet of the trunnion 123 and the width of the trunnion 123 is 9~11；The circular arc
The ratio between width of the arc length of connected in star bottom and the trunnion 123 is 2.8~3.5.
As an example, the diffuser pipe includes lateral wall and inner sidewall, the inner sidewall is close to the axisymmetric cavity knot
The symmetry axis of structure；The inner sidewall includes first part interconnected and second part, and the first part is close to the expansion
The entrance of pressure pipe, and parallel with the lateral wall, the second part close to the outlet of the diffuser pipe, and with the outside
Wall forms 5 °~20 ° of angle；The ratio between width of the width of the entrance of the diffuser pipe and the trunnion is 2~10, the expansion
The ratio between width of the width of the outlet of pressure pipe and the trunnion is 2~10.
As an example, the diffuser pipe 127 includes lateral wall and inner sidewall, the inner sidewall is close to the axisymmetric cavity
The symmetry axis of structure；The inner sidewall includes first part interconnected and second part, and the first part is close to described
The entrance of diffuser pipe 127, and parallel with the lateral wall, the second part close to the outlet of the diffuser pipe 127, and with
The lateral wall forms the angle of certain angle, and the angle for the angle that the second part and the lateral wall are formed is 5 °~
20°；The ratio between width of the width of the entrance of the diffuser pipe 127 and the trunnion 123 is 2~10, and the diffuser pipe 127 goes out
The ratio between width and the width of the trunnion 123 of mouth are 2~10；Preferably, in the present embodiment, the second part and described outer
The angle for the angle that side wall is formed is 8 °~12 °, the width of the width of the entrance of the diffuser pipe 127 and the trunnion 123 it
Than being 2.5~3.5, the ratio between width of the width of the outlet of the diffuser pipe 127 and the trunnion 123 is 3~3.5.
As an example, the algae solution jet pipe 121, gas suction chamber 122, trunnion 123, air inlet cavity 124, feedback channel
125, vibrate chamber 126, the height having the same of diffuser pipe 127, the algae solution jet pipe 121, gas suction chamber 122, trunnion 123,
Air inlet cavity 124, feedback channel 125, oscillation chamber 126, diffuser pipe 127 collectively form the chamber of the oscillating jet mixer 12
The ratio between width of body, the height of the cavity and the trunnion 123 is 0.5~10；Preferably, in the present embodiment, the cavity
Height and the ratio between the width of the trunnion 123 be 3~5.
As an example, the algae solution jet pipe 121, gas suction chamber 122, trunnion 123, air inlet cavity 124, feedback channel
125, vibrating chamber 126 and the formation cavity of diffuser pipe 127 can be used organic glass and is made using laser cutting, the cavity
Top and bottom are all made of organic glass and cover and be fastened by bolts.
As an example, the inside of the reactor body 5 includes positioned at the culture liquid zone 6 and superposed gas of lower part
Disengagement zone 7, the culture liquid zone 6 are used for the culture of algae solution, gas of the gas Disengagement zone 7 for being discharged in the algae solution
Separation.
As an example, the link position of the culture solution external circulation line 10 and the side wall of the reactor body 5 can be with
It sets according to actual needs, it is preferable that the culture solution external circulation line 10 is connected with the top of 5 side wall of reactor body
It connects, the distance of the bottom of reactor body 5 described in the positional distance of connection is the height 0.5~0.8 of the reactor body 5；
It is further preferable that the culture solution external circulation line 10 is reacted with the link position of 5 side wall of reactor body apart from described
The distance of the bottom of device main body 5 is the height 0.6~0.7 of the reactor body 5.
It should be noted that be arranged anyway, need to guarantee the culture solution external circulation line 10 and the reactor
Side wall corresponding to the culture liquid zone 6 in main body 5 is connected, to ensure that the culture solution external circulation line 10 can be with institute
The culture solution stated in reactor body 5 is connected.
As an example, the top and bottom of the reactor body 5 are hemispherical, the height of the reactor body 5
It is 3~20 with the hemispheric diameter ratio；Preferably, in the present embodiment, the height of the reactor body 5 and described half
The ratio between spherical diameter is 10~15.
As an example, the oscillating jet microbubble bioreactor further include: culture solution import 1, culture solution outlet
2, exhaust outlet 4, inner light source pipe 8 and water circulating pump 11；
The culture solution import 1 and the exhaust outlet 4 are located at the top of the reactor body 5, and with the reactor
The inside of main body 5 is connected；Culture solution outlet 2 is located at the bottoms of the reactor body 5, and with the reactor body
5 inside is connected；The top of the inner light source pipe 8 from the reactor body 5 is inserted perpendicularly into the reactor body 5
Portion；The entrance of the water circulating pump 11 is connected with the culture solution external circulation line 10, follows outside the culture solution suitable for driving
The outlet of culture solution in endless tube road 10, the water circulating pump 11 is connected with the entrance of the algae solution jet pipe 121.
As an example, the reactor body 5 can be but be not limited only to perspex transparent material tank body；The reaction
Device main body 5 and the gas distributor 9 and the coaxial distribution of the inner light source pipe 8.
As an example, the reactor body 5 and the inner light source pipe 8 are cylinder, and the inner light source pipe 8 is straight
The diameter ratio of diameter and the reactor body 5 is 5~20；Preferably, the diameter of the inner light source pipe 8 and the reactor master
The diameter ratio of body 5 is 8~12.
As an example, the inner light source pipe 8 is internally provided with internal light source, the internal light source can for providing luminous energy
Think artificial light source (such as LED), or the distributor of natural light made of guide-lighting good material.
As an example, the material of the inner light source pipe 8 and the reactor body 5 is transparent material, as organic glass,
Unorganic glass, transparent plastic etc..
As an example, the outside of the reactor body 5 is also provided with external light source (not shown), the external light source
It can be natural light or artificial light source.
The oscillating jet microbubble bioreactor of the invention is suitable for but is not limited only to for carrying out microdisk electrode
And carbon dioxide fixation.
The working principle of oscillating jet microbubble bioreactor provided by the present invention are as follows: before reactions, will train
Nutrient solution and a certain proportion of frond inject the reactor body 5 from the culture solution import 1 on 5 top of reactor body
Interior, the elemental height of the culture liquid zone 6 should not have the entrance of the culture solution external circulation line 10.Then, it is followed described in unlatching
Ring water pump 11, culture solution enters the oscillating jet from the external circulation line 10 and mixes under the driving of the water circulating pump 11
In device 12, when culture solution is flowed out from the algae solution jet pipe 121 of the oscillating jet mixer 12, flow velocity increases pressure and becomes smaller
In the gas suction chamber 122 formed negative pressure gas injection to be processed is come in through air inlet cavity 124, with culture solution together into
Enter in the oscillation chamber 126.Due to the oscillation chamber 126 be it is geometrically symmetric, when fluid flow out from the trunnion 123, flow
The side wall surface cocurrent that the wall attachment effect of body can be attached at random in the oscillation chamber 126 enters the diffuser pipe 127 of the side,
Fluid flows out 127 rear portion of diffuser pipe and flows into the reactor body 5 through air inlet pipe, and a part is through the feedback flow
Pipeline 13 and the feedback channel 125 are returned in the oscillation chamber 126, and the entrance of feedback flow is located at the entrance of oscillation chamber
Place, belongs to Flow sensitive region here, mainstream by seldom interference will unstability, the mainstream after unstability can pushing away in feedback flow
Dynamic lower deflection is attached in another side wall surface and thus enters the diffuser pipe 127 of the side, due to symmetrical configuration, equally
, side feedback flowing can make mainstream deflection go back again, material is thus formed the deflection oscillator of mainstream (as shown in Fig. 3 to Fig. 7),
To keep the gas of sucking sufficiently broken and be vigorously mixed with liquid.By in the oscillation chamber 126 gas-liquid carry out it is sufficient
Mixing early period, then gas distributor 9 is sent by the admission line 14 together, gas-liquid mixed is equal under the action of oscillating jet
It is even and generate oscillating gas entry, so that bubble is not persistently become larger at gas distribution holes, since oscillation action makes bubble in growth
It generates and shrinks and accelerate to be detached to generate microbubble effect.Gas jet kinetic energy drives the algae solution in reactor to rise flowing,
When reaching culture 6 top of liquid zone, gas is escaped out from culture solution, by the gas Disengagement zone 7 via the exhaust
Mouthfuls 4 are discharged, while culture solution declines along the wall peripheral of the reactor body 5, when to the bottom of the reactor body 5 again
Rise with microbubble jet stream, forms circulation repeatedly.Meanwhile using culture solution external circulation line 10 by the reactor body 5
Interior part culture solution extraction, first carries out gas-liquid mixed early period with gas to be processed outside the reactor body 5, then send again
The efficient photosynthesis for entering to carry out frond and microbubble under light illumination in the reactor body 5, in this way, in addition to the reactor
Culture solution generates interior circulation under the driving of microbubble jet stream in main body 5, and outer follow also is formd outside the reactor body 5
Circulation is dynamic, while the micro-nano bubble that the oscillating jet mixer 12 generates improves gas-liquid mixed and mass transfer effect to the maximum extent
Rate, while reducing gas supply energy consumption.
CO is used in one embodiment2The industrial discharge gas of concentration about 25% obtains after dedusting washing, pressurization, regulating the qi flowing in the channels
Meet the CO of microalgae demand2To be processed gas of the concentration up to 15~20 (percents by volume)；The microalgae can select chlorella
And cyanobacteria.
Gas to be processed is inhaled by air supply opening 3 the gas suction chamber 122 of the oscillating jet mixer 12 due to negative pressure,
Then enter in the oscillation chamber 126 with algae solution and be sufficiently mixed, then a large amount of micro- gas are generated by the gas distributor 9
Bubble, gas flow are that (wherein vvm is ventilation ratio to 0.1-0.5vvm, is logical per minute relative to the volume of the reactor body 5
Enter the amount of gas and the ratio of reactor body volume), since gas-liquid carried out mixing early period in the oscillation chamber 126, then
Connect the gas distributor 9 by the air inlet pipeline 14, under the double action of oscillating jet and gas-liquid mixed, gas with
Very small bubble form exists, and can get extremely efficient mass transfer area, and being uniformly distributed for microbubble promotes CO in gas2
Mass transfer, the CO being dissolved in culture solution2The microalgae biomass of utility value is converted by photosynthesis by microalgae, simultaneously
Release O2, fixed CO2The O generated in the process2, unused inert gas (such as nitrogen) and loose CO2By described
Gas Disengagement zone 7 is discharged through the exhaust outlet 4 of 5 upper end of reactor body.It is first in the operation of microbubble bioreactor
The algae seed liquor that reaction volume device is 20% volume is added in phase, is continuously added to after steady operation by the culture solution import 1
Fresh training base, while in the culture solution for continuously releasing same traffic by culture solution outlet 2, dilution rate is 0.5/d (for liquid
Reactor inner volume is divided by flow rate when flowing into reactor), so that whole work process is in stable volume and stable frond
Biological concentration (such as: OD685 value, i.e., the maximum light absorption value of algae solution is up to 2.8).Contain fixed CO in the culture solution of releasing2Process
The biomass of middle generation is together with culture solution through 2 discharge of culture solution outlet.The temperature of microbubble bioreactor controls
Pass through the 5 outside gravity-flow ventilation of reactor body or spray.Luminous energy is provided by the inner light source pipe 8, can be used artificial
The distributor of natural light can also be made in light source such as LED of guide-lighting good material.
In conclusion the present invention provides a kind of oscillating jet microbubble bioreactor, the oscillating jet microbubble
Bioreactor includes: reactor body, gas distributor, culture solution external circulation line, air supply pipe, oscillating jet mixer
And air inlet pipeline.By adding oscillating jet mixer, culture solution and gas, which are first introduced into the oscillating jet mixer, to be filled
Divide mixing, a large amount of microbubbles is generated under oscillating jet effect using gas distributor, since microbubble is in same air inflow
Under can significantly improve gas liquid interfacial area, to play the important function for strengthening alternate transmittance process, reach efficient carbon sequestration and
The purpose of microdisk electrode；Oscillating jet mixer of the invention compares common normal pressure air inlet, and gas supply energy consumption can be significantly reduced.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (13)
1. a kind of oscillating jet microbubble bioreactor, which is characterized in that the oscillating jet microbubble optical-biological reaction
Device includes: reactor body, gas distributor, culture solution external circulation line, air supply pipe, oscillating jet mixer and air inlet pipe
Road；
The oscillating jet mixer include: algae solution jet pipe, gas suction chamber, trunnion, air inlet cavity, feedback channel, oscillation chamber,
Diffuser pipe, feedback flow pipeline and three-way connection；
The outlet of algae solution jet pipe is via in the entrance insertion gas suction chamber of gas suction chamber；The outlet of gas suction chamber and trunnion
Entrance be connected, the outlet of trunnion is connected with the entrance of diffuser pipe；The air inlet cavity is located at the gas suction chamber
Side, and the outlet of air inlet cavity is connected with the gas suction chamber；Feedback channel is located at the two sides of the trunnion, and feeds back
The outlet of pipeline is connected with the outlet of trunnion；The oscillation chamber is connected with the entrance of diffuser pipe；The feedback flow pipeline
One end is connected via the three-way connection with the outlet of diffuser pipe, and the other end is connected with the entrance of feedback channel；The algae
Liquid jet pipe, gas suction chamber, trunnion, feedback channel, oscillation chamber and diffuser pipe form axisymmetric cavity structure；
The gas distributor is located at the inside of the reactor body, and is located at the bottom of the reactor body；The training
One end of nutrient solution external circulation line is connected via the side wall of the reactor body with the inside of the reactor body, another
End is connected with the entrance of algae solution jet pipe；The air supply pipe is connected with the entrance of air inlet cavity；Air inlet pipeline one end warp
It is connected by the three-way connection with the outlet of the diffuser pipe, the other end is connected with the gas distributor.
2. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the feedback channel,
The quantity of the diffuser pipe and the feedback flow pipeline is two, and the oscillation chamber is between described two diffuser pipes.
3. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the algae solution jet pipe
The ratio between width of the width of entrance and the trunnion is 1~5, the width of the outlet of the algae solution jet pipe and the width of the trunnion
The ratio between be 0.2~0.8, the ratio between the length of the algae solution jet pipe and the width of the trunnion are 2~10.
4. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the gas suction chamber
Side wall be inclined surface, the angle that opposite two sidewalls are formed is 10 °~60 °；The outlet of the gas suction chamber and the algae
The outlet of liquid jet pipe is in the same plane.
5. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the width of the trunnion
Greater than the size of microalgae, the ratio between the length of the trunnion and width are 2~10.
6. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the air inlet cavity
Outlet is located between the entrance and outlet of the algae solution jet pipe；The entrance of the air inlet cavity is circle, and the air inlet cavity enters
Mouthful diameter and the ratio between the width of the trunnion be 1~5, the width of the width of the outlet of the air inlet cavity and the trunnion it
Than being 0.5~5.
7. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the feedback pipe enters
Mouthful width and the ratio between the width of the trunnion be 2~10, the width of the width of the outlet of the feedback pipe and the trunnion it
Than being 0.1~2.
8. oscillating jet microbubble bioreactor according to claim 2, it is characterised in that: the oscillation chamber includes
Flat sidewall positioned at two sides and the circular groove bottom that is connected with the flat sidewall；The planar side positioned at two sides
The junction of wall from the entrance of the outlet of the trunnion, the outlet of described two feedback channels and described two diffuser pipes extends to
In the diffuser pipe；The flat sidewall positioned at two sides forms 20 °~90 ° of angle.
9. oscillating jet microbubble bioreactor according to claim 8, it is characterised in that: the oscillation chamber is axis
Symmetrical structure, the outlet face of the circular groove bottom and the trunnion；The circular groove bottom is to the trunnion
The ratio between width of the distance of outlet and the trunnion is 5~15；The arc length of the circular groove bottom and the trunnion
The ratio between width be 1~5.
10. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the diffuser pipe packet
Include lateral wall and inner sidewall, symmetry axis of the inner sidewall close to the axisymmetric cavity structure；The inner sidewall includes mutual
The first part of connection and second part, the first part close to the diffuser pipe entrance, and it is equal with the lateral wall
Row, the second part is close to the outlet of the diffuser pipe, and the angle with 5 °~20 ° of lateral wall formation；The diffusion
The ratio between width of the width of the entrance of pipe and the trunnion is 2~10, the width of the outlet of the diffuser pipe and the trunnion
The ratio between width is 2~10.
11. oscillating jet microbubble bioreactor according to claim 1, it is characterised in that: the algae solution jet pipe,
Gas suction chamber, trunnion, air inlet cavity, feedback channel, oscillation chamber, diffuser pipe constitute the cavity of the oscillating jet mixer,
The ratio between width of the height of the cavity and the trunnion is 0.5~10.
12. oscillating jet microbubble bioreactor according to any one of claim 1 to 11, it is characterised in that:
The oscillating jet microbubble bioreactor further include: culture solution import, culture solution outlet, exhaust outlet, inner light source pipe and
Water circulating pump；
The culture solution import and the exhaust outlet are located at the top of the reactor body；The culture solution outlet is located at described
The bottom of reactor body；The inner light source pipe is inserted perpendicularly into the reactor body from the top of the reactor body
Portion；The water circulating pump is connected to the culture solution outer circulating tube road.
13. oscillating jet microbubble bioreactor according to claim 12, it is characterised in that: the reactor master
Body and the inner light source pipe are cylinder, and the diameter ratio of the diameter of the inner light source pipe and the reactor body is 5
~20.
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| CN109731490A (en) * | 2018-08-21 | 2019-05-10 | 北京环域生态环保技术有限公司 | A kind of the nano bubble method for generation and device of secondary pressurized multiple stage crushing |
| CN110420609B (en) * | 2019-09-02 | 2024-02-20 | 杭州烃能科技研究有限公司 | Micro-nano scale multiphase flow process strengthening reaction device |
| CN110668522A (en) * | 2019-10-17 | 2020-01-10 | 中南大学 | Micro-nano bubble generating device and micro-nano bubble processing method for oily wastewater |
| CN111718852B (en) * | 2020-07-07 | 2023-05-26 | 浙江佰瑞拉农业科技有限公司 | Automatic change algae and separate fungus culture apparatus |
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