CN106305426A - Establishment method for tissue culture system of Taiwan morus laevigata - Google Patents
Establishment method for tissue culture system of Taiwan morus laevigata Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an establishment method for a tissue culture system of Taiwan morus laevigata. Morus laevigata is a special main tree species in southern China, seedlings of morus laevigata are mainly cultured in traditional modes of cutting, grafting, seeding and the like at present, and the defects that the seedling culturing cost is high, the nursery-stock growth vigor is poor and uneven, and the improved variety potential cannot be fully developed exist. According to the establishment method, excellent clone twigs serve as explants, in-vitro regeneration plants of morus laevigata are obtained through the explant sterilizing process, the inducing culturing process, the cluster shoot proliferation process, the adventitious root formation process, the acclimatization and transplant process and the like, the tissue-culture rapid propagation technology system of morus laevigata is established, the excellent characters of clone female parents of morus laevigata can be kept, and large-scale production of excellent clone nursery stocks of morus laevigata is facilitated. Fruits obtained with the establishment method can be eaten, mulberry leaves are used for feeding and breeding silkworms, and fruits can also be used for making wine, cakes and beverages. The establishment method has the advantages of being simple and practicable in technology method, easy to operate, good in growth vigor and the like.
Description
Technical field
The present invention relates to morus laevigata breeds field, the method for building up of a kind of Taiwan nurture system of morus laevigata group.
Background technology
Taiwan morus laevigata is to be bred as after the hybridization of wild morus laevigata with Taiwan common fruit Mulberry by Taiwan agriculture expert. cause and effect
Mulberry is generally up to 12 cm, the longest gains the name up to 20 centimetres.Maximum single fruit weight 20 grams, sugar content about 20 degree.Nothing
Tart flavour. mouthfeel is dense sweet like honey. and nutritious.Cultivate then and get final product result autumn then. the most annual repeatedly result. high yield period mu
To produce general up to 3000 ~ 3500 kilograms. Super-high-yielding plot is up to more than 4500 kilograms, and yield is general about 2 times of Mulberry of fruit.These product
Kind solve and overcome tradition fruit Mulberry because of the least, tart flavour heavy and yield is the lowest. be difficult to develop plantation as fruit variety
Shortcoming. make fruit Sang Jinhang scale establishing in large scale.Development and Production fruit Mulberry wine, really Mulberry juice, really Mulberry cream and other products become
Reality.
Summary of the invention
It is an object of the invention to provide a kind of is outer implant with high-quality clone, by outer implant sterilization, inducing culture, grows thickly
Shoot propagation, adventitious root germinate, seedling exercising is transplanted and obtained in vitro plant again, complete the foundation of system.Really Mulberry has that fruit is big, knot
The distinguishing features such as fruit is early, yield is high, plant is downgraded, management is simple, market development has a extensive future, China's most area all can be planted
Plant.The fruit Mulberry series new varieties the most just starting to promote have the short row kind in Taiwan, long row kind, domestic big ten, 8632 etc..Really Mulberry
Third generation fruit as sight seeing and pastime farm and processing fresh Fructus Mori juice beverage and food is extensively planted, by consumer
Extensive welcome with Producer.
The technical solution of the present invention is such, the method for building up of a kind of Taiwan nurture system of morus laevigata group, its feature
It is to comprise the following steps:
Step 1, outer implant collection and sterilization: take the full twig giving birth to the state of sprouting then as outer implant, in flowing water undershoot
Washing 6h, be soaked in 7% washing powder solution 12 minutes, banister brush 7% washing powder solution washing material gently, tap water is to drip
Form rinses 97min, and distilled water flushing 7 times, with 78% alcohol solution dipping 70s, aseptic water washing 7 in superclean bench
Secondary, containing 0.8% mercuric chloride solution sterilization 17min of 0.09% tween 20, after aseptic water washing 7 times, blot surface with aseptic filter paper
Moisture standby;
Step 2, inducing culture: the twig after step (1) processes is cut into the stem section of about 1.8cm and is inoculated into inducing culture
Carrying out inducing clumping bud cultivation, illumination every day 16 hours after inoculation, intensity of illumination is 3000lx, and cultivation temperature is 26 DEG C, air
Relative humidity be cultivate 37 days under conditions of 78% after add up induction situation;
Step 3, adventitious buds proliferation: step (2) inducing culture is obtained Multiple Buds and is inoculated into proliferated culture medium and carries out adventitious bud increasing
Growing cultivation, inoculation is placed on illumination every day 16 hours, and intensity of illumination is 3000lx, and being placed in cultivation temperature is 26 DEG C, and air is relative
Humidity be cultivate 45 days under conditions of 83% after add up germinative number;
Step 4, root culture: the plant obtained in step (3) being bred is cut from base portion and is seeded to root media enter
Row root induction, illumination every day 16 hours after inoculation, intensity of illumination is 3400lx, and cultivation temperature is 26 DEG C, relative air humidity
It is that after cultivating 37 days under conditions of 86%, statistics is taken root situation;
Step 5, acclimatization and transplants: the bottle Seedling possessing transplanting condition obtained on root media is carried out seedling exercising, and bottle Seedling moves to often
Temperature, after lower 7 days, is opened bottle cap 5 days, is then washed away and be attached to the culture medium that shoot root is fastened, in the bacteria agent of 1000 times
Soaking 17min, then transplant and cultivate to the container bag fill designed transplanting culture matrix, each container bag is moved
Planting 1 strain to take root Seedling, the hot house inside holding moisturizing temperature being placed in the auto spraying with shade net controls at 37 DEG C, humidity
About 89% should be maintained at, it is to avoid direct sunlight, after transplanting 35 days, add up survival rate.
Inducing culture described in step (2) is: MS+1.3mg/L NAA+8.4mg/L 6-BA+3.6mg/L KT+
1.65g/L AC+36g/L sucrose+6.5g/L agar, pH is 5.9.
Proliferated culture medium described in step (3) is: MS+0.7mg/L 2,4-D+6.5mg/L 6-BA+1.4mg/L NAA+
1.8g/L AC+ 35g/L sucrose+6.5g/L agar, pH is 5.4～5.9.
Root media described in step (4) is: White+1.3mg/L NAA+3.3mg/L IBA+35g/L sucrose+8g/
L agar, pH is 5.9.
Transplanting culture matrix described in step (5) is for by peat soil: Vermiculitum: Margarita charcoal: coconut palm bran=1:3:2:2 mixes
Substrate.
Compared with prior art the prominent effect of the present invention is: overcome the cuttage that fruit Sang Chuantong uses, grafting and seeding method
Nursery, the shortcomings such as seedling cost is low, long potential difference, uneven.The present invention has simple for process, simple to operate, low cost,
Breed Seedling easily to grow.The fruit that the application present invention obtains can be used as fruit food, and Folium Mori are fed silkworm and bred silkworms.Fruit also can process fruit wine, really
Mulberry cream, really Beverage of mulberry etc..
Detailed description of the invention
It is described in detail below according to morus laevigata characteristic embodiment.
Embodiment 1
1. build garden and cultivation requires: this kind uses fruit Mulberry and wild morus laevigata cross breeding. there is extremely strong distant hybrid
Advantage. the domestic any soil property in area having growing of mulberry all can be planted.But build high yield garden should select that soil layer is deep, soil fertility fertilely
Block is planted.Field planting ditch depth 50 centimetres. wide 60 centimetres, bottom of trench one layer of crops stem stalk of paving, thickness 20-30 centimetre, covers 10 centimetres of left sides
Right table soil, again with farmers' fowl, poultry or night soil on table soil. backfill whole table after mu import compound fertilizer 50 kilograms more native. and field planting
Seeding row spacing is in 2 meters × 3 meters of fertile plot, and mu plants 111 strains.Lean unfertile land, hillside, dust storm wasteland seeding row spacing 1 meter × 2 meters. mu is planted
333 strains.Field planting requires to be as good as with general fruit tree. notice that ridging is treaded rear speed and watered and determine root water. and arid seriously also should repeatedly be watered in area
Water all survives to protect once to cultivate.
2. management technique main points: surely do away from ground 30 centimeters cutting back nursery stock after field planting. the most general every strain can be sprouted
Young sprout 6-8. pinch the top heart during young sprout length to 20 centimetre and promote branch.Field planting should not advocated promote it in a large number based on foster tree then
Result. but also can pick fresh fruit in the fall.Second Year can pick 3 ~ 4 batches of fresh fruit at the Yellow River and Huai He River to basin, Changting. and high yield strain can reach 25
About kilogram.Fruit 5-6 stubble can be received in Guangdong, Guangxi and Hainan, and yield is higher.After often having adopted one batch of fruit, cutting back bearing basal shoot immediately.
Stay bud 2-3 to promote it to sprout and make next batch of bearing basal shoot. and execute quick-acting fertilizer as early as possible or carbamide promotees the growth of its flower bud germination. for next
Stubble fruit Mulberry high yield creates conditions.After annual autumn has been adopted last batch of autumn fruit in various places. should be field planting interline trench digging heavy dressing coming year
Base manure .. applies fertilizer specifically and answers heavy dressing farm organic fertilizer and dung polluted water etc. it is aided with import compound fertilizer 50 kilograms every mu again. and promote bud and divide
Turn to again get bumper crops the coming year and lay the first stone.For improving yield and quality, it is possible in each early flowering season to young fruit period. foliage-spray phosphorus
Acid diamino potassium and nature brown cotton mixed liquor. it is remarkably improved sugar content. promote precocity, stable yields, volume increase.
3. insect pest preventing and controlling: this kind because of be use the hybridization of wild morus laevigata after be bred as.Show extra-heavy resistance against diseases.
Layout to plant experimentally in China and all show high anti-sclerotiniose and powdery mildew, also do not find other diseases.The general time is not required to pesticide
Controlling disease. as found insect pest. local low residual hazard pesticide can be used to control worm.
4. gather: this kind early goes public about 10 days than common fruit Mulberry, typically ripe in late April.Because of the sugary height of this kind.
When fruit Mulberry by green turn red time can gather listing. red to purple black time pol content the highest.Quality also reaches the most excellent.Gather with product in early morning
Matter is optimal.
Embodiment 2
(1), biological characteristics: really mulberry gesture is strong, grows vigorous, and in a year, new branch constantly produces, the multilamellar side shoot of formation
Being Second Year fruitful branch, germination percentage is up to about 98%, and each sprouting all produces 3-6 sorosis, mean fruit weight 4.5 grams, really
Real big and even, in puce.Fruit type is neat, in good taste, ripe neat.Within 4 years, raw maximum individual plant produces fruit 26 kilograms, and per hectare produces sorosis
45 tons；Under natural conditions, every annual bearing 2 times, autumn, yield accounted for the 20% of spring, especially high yield, stable yields.Skin ash sepia, skin
Hole is thick and close；Hibernaculum is loose, ovalize, yellowish-brown, suitable with raw branch thickness；Blade is little, thick, glossy.
(2), Cultural technique:
1, selection of land builds garden: the knob of low altitude area, and the various soil of plains region all can be planted, and wherein irrigation and drainage are convenient,
Soil property fertile soil growing way is prosperous, and yield is high.Digging field planting ditch, ditch depth 50 centimetres before field planting, wide 60 centimetres, bottom of trench spreads one layer 20 lis
The Caulis et Folium Oryzae that rice is thick, overlying 10 cm table soil, table soil is executed chicken manure, compound fertilizer again, then backfills.Every mu covers Caulis et Folium Oryzae 1.5 altogether
Ton, chicken manure 2 tons, compound fertilizer 50 kilograms or every mu execute high-quality well-rotted farmyard manure 3 tons, and field planting distance between rows and hills is 3 meters × 2 meters, every mu of kind
111 strains.Barren ground seeding row spacing 1 meter × 2 meters, plants 333 strains for every mu.During field planting, nursery stock is put into the field planting ditch filled and led up, and by root
System makes in order and makes it stretch to surrounding, and then ridging is treaded, and waters the most permeable, then spreads Caulis et Folium Oryzae or plastic sheeting, anti-mud at the tree end
Soil pollutes sorosis.
2, pruning: the nursery stock after field planting is doing surely away from ground 20-25 centimeters cutting back.Young sprout 5-6 is sprouted in the most general every strain
Individual, pinching during young sprout length to 20 centimetre, large result branch can be produced；After Second Year fruit picking terminates, carry out repairing summer in conjunction with shaping
Cutting, control trunk height, cut off too much descending branch, all of bearing basal shoot all stays 2-3 bud cutting back, promotees it and sprouts young sprout, makees
Bearing basal shoot for the coming year.Cutting back is urgent, and to ensure that young sprout has the time growth of abundance, accumulation nutrition carries out bud and divides
Change.Annual all cutting backs after Mulberry is very gathered, gradually form low dry tree-like.By small and weak branch, the degeneration of sprouting in summer during winter pruning
The suitable cutting back of bearing basal shoot, typically cut off 20-25 centimetre of branch top top.
3, water and fertilizer management: after really Mulberry needs water phase mainly spring pruning, budding period and summer cut the rear budding period, the two should be mended period in time
Water-filling divides.Annual winter is same " building garden standard " in field planting interline ditching-fertilizing, fertilization mode and dose.After early summer in June cuts
Want heavy dressing fertilizer, optimum with chicken manure, soybean cake, be aided with import compound fertilizer, carbamide (20 kilograms every mu), promote bud differentiation and
Formed.Early flowering season and young fruit period carry out foliage-spray 1 time respectively, and the potassium dihydrogen phosphate that can be selected for 0.25% mixes with nature brown cotton
Closing liquid, can significantly improve the sugar content of fruit, promote precocity, the big color of fruit is gorgeous, and stable yields is increased production.
4, the prevention and control of plant diseases, pest control: harm fruit the disease main foxiness disease of Mulberry, anthrax, powdery mildew etc..Insect mainly have mulberry geometrid (Hemerophila atrilineata),
Euproctis similis, hishimonus sellatus, mulberry borer etc..After the dry branches and fallen leaves pruned is burned by annual winter, buried in conjunction with fertilising；Use before rudiment
Full branch is carried out disinfection by the lime sulfur of 3 Baume degrees；The 7-9 month high temperature and rainy phase, sprayed 1 time 40% oxidation every 10-15 days happy
Really 1200 times of liquid or 2000 times of liquid of decis add 1200 times of liquid of 75% thiophanate methyl or 800 times of liquid of 75% Bravo, to prevent and treat Mulberry
Caterpillar, brown spot etc..If finding mulberry borer harm branch, larva can use channel injection or fill in swab and kill, adult can
Artificial capture method.
Embodiment 3
(1) outer implant collection and sterilization: take the full twig of sorosis tree raw state of sprouting then as outer implant, under flowing water
Rinse 4h, be soaked in 5% washing powder solution 9min, with banister brush 5% washing powder solution washing material gently, then with tap water with
The form dripped rinses 58min, with distilled water flushing 6 times, with 75% alcohol solution dipping 30s in superclean bench, aseptic
Water rinses 8 times, then with the 0.3% mercuric chloride solution sterilization 6min containing 0.05% tween 20, uses aseptic filter after aseptic water washing 8 times
The moisture that paper blots surface is standby.
(2) inducing culture: the twig after step (1) processes is cut into the stem section of about 4cm and is inoculated into inducing culture
Carry out inducing clumping bud cultivation.Inoculation is placed on illumination every day 16 hours, and intensity of illumination is 1800lx, and cultivation temperature is 25 DEG C,
Relative air humidity is that after cultivating 36 days under conditions of 75%, inductivity reaches 100%.Described inducing culture is: MS+0.5mg/L
NAA+3.0mg/L 6-BA+3mg/L KT+1.5g/L AC+28g/L sucrose+7.0g/L agar, pH is 5.6.
(3) adventitious buds proliferation: step (2) inducing culture is obtained Multiple Buds and is inoculated into proliferated culture medium and carries out adventitious bud increasing
Grow cultivation.Illumination every day 16 hours after inoculation, intensity of illumination is 1800lx, and being placed in cultivation temperature is 25 DEG C, relative air humidity
Be cultivate 45 days under conditions of 75% after bud number more than 8.Described proliferated culture medium is: MS+0.5mg/L 2,4-D+5.5mg/
L 6-BA+0.8mg/L NAA+1.5g/L AC+ 28g/L sucrose+7.5g/L agar, pH is 5.6.
(4) root culture: the plant obtained in step (3) being bred is cut 4cm from base portion and is seeded to root media
In carry out root induction.Illumination every day 16 hours after inoculation, intensity of illumination is 2500lx, and cultivation temperature is 25 DEG C, and air is relative
Humidity be cultivate 36 days under conditions of 75% after take root and reach more than 96%.Described root media is: White+0.6mg/L
NAA+2.0mg/L IBA+25g/L sucrose+4.8g/L agar, pH is 5.6.
(5) acclimatization and transplants: the bottle Seedling possessing transplanting condition obtained on root media is carried out seedling exercising, and bottle Seedling moves to often
Temperature, after lower 4 days, is opened bottle cap 5 days, is then washed away and be attached to the culture medium that shoot root is fastened, in the bacteria agent of 1000 times
Soaking 8min, then transplant and cultivate to the container bag fill designed transplanting culture matrix, each container bag is transplanted
1 strain is taken root Seedling.The hot house inside holding moisturizing temperature being placed in the auto spraying with shade net controls at 28 DEG C, and humidity should
Being maintained at about 75%, it is to avoid direct sunlight, after transplanting 35 days, survival rate is to more than 93.Described transplanting culture matrix is
By peat soil: Vermiculitum: Margarita charcoal: coconut palm bran=1:2:3:3 mixes substrate.
Embodiment 4
(1) outer implant collection and sterilization: take raw then the full tender of state of sprouting that fir pile foundation portion Morphological Characterization is consistent
Branch, as outer implant, rinses 6h under flowing water, is soaked in 6% washing powder solution 12min, with banister brush 6% washing powder solution gently
Washing material, then rinse 80min with the form dripped with tap water, with distilled water flushing 6 times, in superclean bench with
75% alcohol solution dipping 38s, aseptic water washing 8 times, then with containing 0.04% tween 20 0.4% mercuric chloride solution sterilization 10min,
The moisture on surface is blotted with aseptic filter paper standby after aseptic water washing 8 times.
(2) inducing culture: the twig after step (1) processes is cut into the stem section of about 4cm and is inoculated into inducing culture
Carry out inducing clumping bud cultivation.Inoculation is placed on illumination every day 16 hours, and intensity of illumination is 3000lx, and cultivation temperature is 26 DEG C,
Relative air humidity is that after cultivating 35 days under conditions of 78%, inductivity reaches 98.5%.Described inducing culture is: MS+0.6mg/
LNAA+8.0mg/L 6-BA+4.0mg/L KT+1.6g/L AC+40g/L sucrose+8.0g/L agar, pH is 5.6.
(3) adventitious buds proliferation: step (2) inducing culture is obtained Multiple Buds and is inoculated into proliferated culture medium and carries out adventitious bud increasing
Grow cultivation.Inoculation is placed on illumination every day 16 hours, and intensity of illumination is 3000lx, and cultivation temperature is 26 DEG C, relative air humidity
Be cultivate 45 days under conditions of 75% after bud number more than 11.Described proliferated culture medium is: MS+0.4mg/L 2,4-D+
4.5mg/L 6-BA+0.65mg/L NAA+1.4g/L AC+ 28g/L sucrose+5.5g/L agar, pH is 5.6.
(4) root culture: the plant obtained in step (3) being bred is cut 4cm from base portion and is seeded to root media
In carry out root induction.Illumination every day 16 hours after inoculation, intensity of illumination is 3000lx, and cultivation temperature is 25 DEG C, and air is relative
Humidity be cultivate 36 days under conditions of 75% after take root and reach more than 97.5%.Described root media is: White+1.2mg/L
NAA+2.5mg/L IBA+26g/L sucrose+6.5g/L agar, pH is 5.6.
(5) acclimatization and transplants: the bottle Seedling possessing transplanting condition obtained on root media is carried out seedling exercising, and bottle Seedling moves to often
Temperature, after lower 6 days, is opened bottle cap 6 days, is then washed away and be attached to the culture medium that shoot root is fastened, in the bacteria agent of 1000 times
Soaking 10min, then transplant and cultivate to the container bag fill designed transplanting culture matrix, each container bag is moved
Plant 1 strain to take root Seedling.The hot house inside holding moisturizing temperature being placed in the auto spraying with shade net controls at 25 DEG C, humidity
Should be maintained at about 75%, it is to avoid direct sunlight, after transplanting 36 days, survival rate is to more than 96.5.Described transplanting culture medium
Matter is for by peat soil: Vermiculitum: Margarita charcoal: coconut palm bran=1:3:3:3 mixes substrate.
Claims (5)
1. the method for building up of the Taiwan nurture system of morus laevigata group, it is characterised in that comprise the following steps:
Step 1, outer implant collection and sterilization: take the full twig giving birth to the state of sprouting then as outer implant, in flowing water undershoot
Washing 6h, be soaked in 7% washing powder solution 12 minutes, banister brush 7% washing powder solution washing material gently, tap water is to drip
Form rinses 97min, and distilled water flushing 7 times, with 78% alcohol solution dipping 70s, aseptic water washing 7 in superclean bench
Secondary, containing 0.8% mercuric chloride solution sterilization 17min of 0.09% tween 20, after aseptic water washing 7 times, blot surface with aseptic filter paper
Moisture standby;
Step 2, inducing culture: the twig after step (1) processes is cut into the stem section of about 1.8cm and is inoculated into inducing culture
Carrying out inducing clumping bud cultivation, illumination every day 16 hours after inoculation, intensity of illumination is 3000lx, and cultivation temperature is 26 DEG C, air
Relative humidity be cultivate 37 days under conditions of 78% after add up induction situation;
Step 3, adventitious buds proliferation: step (2) inducing culture is obtained Multiple Buds and is inoculated into proliferated culture medium and carries out adventitious bud increasing
Growing cultivation, inoculation is placed on illumination every day 16 hours, and intensity of illumination is 3000lx, and being placed in cultivation temperature is 26 DEG C, and air is relative
Humidity be cultivate 45 days under conditions of 83% after add up germinative number;
Step 4, root culture: the plant obtained in step (3) being bred is cut from base portion and is seeded to root media enter
Row root induction, illumination every day 16 hours after inoculation, intensity of illumination is 3400lx, and cultivation temperature is 26 DEG C, relative air humidity
It is that after cultivating 37 days under conditions of 86%, statistics is taken root situation;
Step 5, acclimatization and transplants: the bottle Seedling possessing transplanting condition obtained on root media is carried out seedling exercising, and bottle Seedling moves to often
Temperature, after lower 7 days, is opened bottle cap 5 days, is then washed away and be attached to the culture medium that shoot root is fastened, in the bacteria agent of 1000 times
Soaking 17min, then transplant and cultivate to the container bag fill designed transplanting culture matrix, each container bag is moved
Planting 1 strain to take root Seedling, the hot house inside holding moisturizing temperature being placed in the auto spraying with shade net controls at 37 DEG C, humidity
About 89% should be maintained at, it is to avoid direct sunlight, after transplanting 35 days, add up survival rate.
The method for building up of a kind of Taiwan nurture system of morus laevigata group the most according to claim 1, it is characterised in that step (2) institute
The inducing culture stated is: MS+1.3mg/L NAA+8.4mg/L 6-BA+3.6mg/L KT+1.65g/L AC+36g/L sucrose+
6.5g/L agar, pH is 5.9.
The method for building up of a kind of Taiwan nurture system of morus laevigata group the most according to claim 1, it is characterised in that step (3) institute
The proliferated culture medium stated is: MS+0.7mg/L 2,4-D+6.5mg/L 6-BA+1.4mg/L NAA+1.8g/L AC+ 35g/L sugarcane
Sugar+6.5g/L agar, pH is 5.4～5.9.
The method for building up of a kind of Taiwan nurture system of morus laevigata group the most according to claim 1, it is characterised in that step (4) institute
The root media stated is: White+1.3mg/L NAA+3.3mg/L IBA+35g/L sucrose+8g/L agar, pH is 5.9.
The method for building up of a kind of Taiwan nurture system of morus laevigata group the most according to claim 1, it is characterised in that step (5) institute
The transplanting culture matrix stated is for by peat soil: Vermiculitum: Margarita charcoal: coconut palm bran=1:3:2:2 mixes substrate.
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| CN108633732A (en) * | 2018-08-03 | 2018-10-12 | 西北农林科技大学 | A kind of mulberry Polyploid Induction Methods based on tissue-cultured seedling |
| CN111903528A (en) * | 2020-09-11 | 2020-11-10 | 湖北省农业科学院经济作物研究所 | Tissue culture regeneration rapid propagation method for medicinal mulberry winter buds |
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