CN106290338B - A method of detection amantadine content - Google Patents

A method of detection amantadine content Download PDF

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Publication number
CN106290338B
CN106290338B CN201610813320.8A CN201610813320A CN106290338B CN 106290338 B CN106290338 B CN 106290338B CN 201610813320 A CN201610813320 A CN 201610813320A CN 106290338 B CN106290338 B CN 106290338B
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China
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amantadine
solution
reaction
cucurbit
urea
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CN201610813320.8A
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Chinese (zh)
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CN106290338A (en
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李根喜
曹亚
韩鹏
王竹馨
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上海大学
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/3103Atomic absorption analysis

Abstract

The invention discloses a kind of methods for detecting amantadine content, the steps include: that a. takes substance withdrawl syndrome is that cucurbit [7] urea solution of 0.25~0.6mM is placed in micro tube, sample solution containing amantadine is added to be reacted, reaction temperature is 20~30 DEG C, reaction 0.5~1 hour, obtains cucurbit [7] urea@amantadine complex solution after reaction;B. ABTS solution and 25 μ L of hydrogenperoxide steam generator are added into resulting cucurbit [7] urea@amantadine complex solution, is heated to 40~50 DEG C, reacts 0.5~1 hour, detection solution is obtained after chromogenic reaction;C. amantadine content is calculated according to the relationship at 420nm wavelength between absorbance value and amantadine concentration using the uv-visible absorption spectra of the detection solution after ultraviolet-visible spectrophotometer measurement chromogenic reaction.This method is easy to operate, quick, and high sensitivity, specificity are good.

Description

A method of detection amantadine content

Technical field

The present invention relates to a kind of methods of quickly detection amantadine content, belong to technical field of analytical chemistry.

Background technique

Amantadine (amantadine), also known as amantadine are a kind of Common drugs molecules with symmetrical structure.Medicine Pharmacological research shows that amantadine can prevent virus from penetrating host cell and effectively inhibit the shelling and breeding of virus;Together When, dopaminergic neuron release dopamine mediator can be promoted in brain tissue and inhibit the reuptake of dopamine.Therefore, Amantadine has important application in terms of antiviral and treatment.But excessive use amantadine The adverse reactions such as drowsiness, dizziness, phonism, nervous system injury can be caused.In order to avoid the excess of amantadine to take the photograph as far as possible Enter, using accurate feasible amantadine detection method, realizes that the Accurate Determining of amantadine content in drug tablet is just shown It obtains particularly important.Currently used amantadine detection method mainly includes high performance liquid chromatography, gas chromatography, mass spectrum Method, capillary electrophoresis and Oscillographic voltammetry etc..Although these methods detection sensitivity, specificity and in terms of it is each Advantageous, still, time-consuming, instrument and equipment is expensive for the generally existing detection of common amantadine detection method, cumbersome etc. Disadvantage.

Summary of the invention

The purpose of the present invention is: for the deficiency of existing method, propose a kind of method for detecting amantadine content, the party The chromogenic reaction that method depends on cucurbit [7] urea (Cucurbit [7] uril) to participate in forms stable amantadine and cucurbit [7] The complex of urea realizes the content detection of amantadine.

To achieve the above object, the present invention uses following mechanism:

(1) ABTS (double -3- ethyl-benzothiazole -6- sulfonic acid of 2,2'- connection nitrogen) is a kind of common peroxidase colour developing Substrate;By the catalysis of peroxidase, ABTS can generate stable radical cation ABTS with hydroperoxidation ·+;Cucurbit [7] urea can improve its reactivity by internal hydrophobic cavity combination ABTS, make it without peroxide Under conditions of enzymatic, ABTS directly is generated with hydroperoxidation+

(2) when amantadine is not present, ABTS can be in conjunction with cucurbit [7] urea, and then ABTS is directly and hydrogen peroxide Reaction generates green matter ABTS+, to occur apparent characteristic absorption peak at uv-visible absorption spectra 420nm.When There are when amantadine, amantadine forms stable cucurbit [7] urea@adamantane by occupying the hydrophobic cavity of cucurbit [7] urea Amine complex, so that ABTS can not cause ABTS directly not generate ABTS with hydroperoxidation in conjunction with cucurbit [7] urea ·+, this causes the color keep for detecting solution colourless, and can not occur apparent ultraviolet-ray visible absorbing peak at 420nm.Root According to absorbance value of the detection solution at 420nm, the detection to amantadine is realized.

According to above-mentioned mechanism, the technical solution adopted by the present invention is that:

A kind of method of quick detection amantadine content, it is characterised in that the specific steps of this method are as follows:

A. taking substance withdrawl syndrome is that the 100 μ L of cucurbit [7] urea solution of 0.25~0.6mM is placed in micro tube, and addition contains 100 μ L sample solution of amantadine, are reacted after being sufficiently mixed, reaction temperature be 20~30 DEG C, the reaction time be 0.5~ 1 hour, cucurbit [7] urea@amantadine complex solution is obtained after reaction;

B. substance withdrawl syndrome is added into the resulting cucurbit of above-mentioned steps (a) [7] urea amantadine complex solution is The 25 μ L of ABTS solution and substance withdrawl syndrome of 30~50mM is the 25 μ L of hydrogenperoxide steam generator of 25~50mM, is uniformly mixed, adds Heat the reaction time 0.5~1 hour, obtains detection solution to 40~50 DEG C of progress chromogenic reactions after chromogenic reaction;

C. existed using the detection solution after ultraviolet-visible spectrophotometer measurement above-mentioned steps (b) resulting chromogenic reaction Uv-visible absorption spectra in 400~500nm wave-length coverage, and according to the absorbance (A at 420nm420) value and Buddha's warrior attendant Relationship between alkanamine concentration calculates amantadine content.

Compared with prior art, the present invention has the advantages that following protrusion:

This method is matched dependent on cucurbit [7] urea@amantadine of chromogenic reaction and stable formation that cucurbit [7] urea participates in Object is closed, the content detection of amantadine is realized;This method is easy to operate, quick, and high sensitivity, specificity are good.

Detailed description of the invention

Fig. 1 is the uv-visible absorption spectra obtained when detecting various concentration amantadine., in figure, from a to i, Buddha's warrior attendant When the concentration of alkanamine is respectively 0 μM, 5 μM, 31.25 μM, 62.5 μM, 125 μM, 150 μM, 175 μM, 200 μM and 225 μM, Characteristic absorption peak at 420nm is gradually decreased with the raising of amantadine concentration, wherein occurs one at 420nm significantly Characteristic absorption peak.

Fig. 2 is the A of detection solution after chromogenic reaction420Relational graph between value and amantadine concentration, can from figure Out, the lowest detection for amantadine detection is limited to 3.81 μM, when the concentration of amantadine is between 5~150 μM, CADA And A420Linear relationship between value;Equation of linear regression is A420=-0.0153CADA+ 2.507 (r=0.998).

If Fig. 3 is to be measured molten after the chromogenic reaction for detecting 200 μM of amantadines and 2000 μM of dry medicament Coexisting components Liquid A420Value is compared to blank control group A420The ratio schematic diagram of value.

Specific embodiment

After now specific embodiments of the present invention are described in.

Embodiment 1

The method that one kind of the invention quickly detects amantadine content, its step are as follows:

A. taking substance withdrawl syndrome is that the 100 μ L of cucurbit [7] urea solution of 0.5mM is placed in micro tube, is added and contains adamantane 100 μ L sample solution of amine, are reacted after being sufficiently mixed, and reaction temperature is 25 DEG C, and the reaction time is 0.5 hour, after reaction Obtain cucurbit [7] urea@amantadine complex solution;

B. substance withdrawl syndrome is added into the resulting cucurbit of above-mentioned steps (a) [7] urea amantadine complex solution is The 25 μ L of ABTS solution and substance withdrawl syndrome of 40mM is the 25 μ L of hydrogenperoxide steam generator of 40mM, is uniformly mixed, is heated to 45 DEG C, Occur chromogenic reaction (reaction time is 0.5 hour), obtains detection solution after chromogenic reaction;

C. using the detection solution after ultraviolet-visible spectrophotometer measurement above-mentioned steps (b) resulting chromogenic reaction Uv-visible absorption spectra in 400~500nm wave-length coverage, according to A420Relationship between value and amantadine concentration, Amantadine content is calculated, as shown in Figure 1 and Figure 2.

In order to verify the method for the present invention test agents amantadine content specificity, if by dry medicament Coexisting component (chlorphenamine maleate, sucrose, glucose, threonine, maltose and alanine) is used as control, according to the step of above-described embodiment Rapid technical solution is detected respectively.Testing result is as shown in Figure 3: when detecting the sample solution containing 200 μM of amantadines, Detection solution A after chromogenic reaction420Value significantly reduces, with blank control group A420Value is compared, and down ratio is up to 95%;And The corresponding A of detection solution when detecting the contrast solution containing 2000 μM of other medicament Coexisting components, after chromogenic reaction420Value It reduces less.These results explanation, this method have specificity well for detecting amantadine.

Claims (1)

1. a kind of method for detecting amantadine content, which is characterized in that the specific steps of this method are as follows:
A. taking substance withdrawl syndrome is that the 100 μ L of cucurbit [7] urea solution of 0.25~0.6mM is placed in micro tube, is added and contains Buddha's warrior attendant 100 μ L sample solution of alkanamine, are reacted after being sufficiently mixed, and reaction temperature is 20~30 DEG C, and the reaction time is 0.5~1 small When, cucurbit [7] urea@amantadine complex solution is obtained after reaction;
B. it is 30 that substance withdrawl syndrome is added into the complex solution of the resulting cucurbit of above-mentioned steps (a) [7] urea amantadine The 25 μ L of ABTS solution and substance withdrawl syndrome of~50mM is the 25 μ L of hydrogenperoxide steam generator of 25~50mM, is uniformly mixed, heating To 40~50 DEG C of progress chromogenic reactions, the reaction time 0.5~1 hour, detection solution is obtained after chromogenic reaction;
C. using the detection solution after ultraviolet-visible spectrophotometer measurement above-mentioned steps (b) resulting chromogenic reaction 400~ Uv-visible absorption spectra in 500nm wave-length coverage, according to the absorbance (A at 420nm420) value and amantadine concentration it Between relationship, calculate amantadine content.
CN201610813320.8A 2016-09-10 2016-09-10 A method of detection amantadine content CN106290338B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106905958B (en) * 2017-03-21 2019-08-02 贵州大学 A kind of fluorescence probe based on trans- cucurbit(7)uril, preparation method and application
CN107502339B (en) * 2017-07-19 2019-10-11 贵州大学 A kind of ratio fluorescent probe identifying nilotinib and its preparation and recognition methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105209910A (en) * 2013-03-14 2015-12-30 百奥马克科技有限公司 Detection and quantification of acetylamantadine in urine samples
CN105319351A (en) * 2014-07-24 2016-02-10 江苏维赛科技生物发展有限公司 Adamantanamine enzyme-linked immunosorbent assay detection special-purpose detection kit

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105209910A (en) * 2013-03-14 2015-12-30 百奥马克科技有限公司 Detection and quantification of acetylamantadine in urine samples
CN105319351A (en) * 2014-07-24 2016-02-10 江苏维赛科技生物发展有限公司 Adamantanamine enzyme-linked immunosorbent assay detection special-purpose detection kit

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Determination of amantadine and rimantadine using a sensitive fluorescent probe;Guang-Quan Wang 等;《Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy》;20120819;第98卷;第275-281页
以葫芦[7]脲与异喹啉类生物碱包结物为荧光探针的药物分析研究进展;张先廷;《辽宁化工》;20141231;第43卷(第12期);第1504-1506页
基于过氧化物模拟酶催化显色反应的博来霉素比色检测;芦珊 等;《分析测试学报》;20140930;第33卷(第9期);第1068-1072页

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