CN106289862B - Brain tissue nuclear cluster extraction instrument - Google Patents

Abstract

The invention discloses a brain tissue nucleus extractor, belonging to the technical field of laboratory equipment, comprising: a fixing groove (14) for containing brain tissue, the fixing groove (14) has a scale value; An extraction device above the fixing groove (14), the extraction device comprising an extraction cylinder (5), an extraction needle arranged under the extraction cylinder (5), and a piston (7) slidably connected to the inner wall of the extraction cylinder (5) ); and a positioning device, which is fixedly connected with the extraction cylinder (5) and drives the extraction cylinder (5) to move vertically and horizontally. The above extractor solves the problem that the existing extraction device cannot accurately obtain the required nuclei, and achieves the purpose of accurately and non-invasively extracting the nuclei of the brain tissue, which is simple, convenient and cost-effective.

Description

Brain Tissue Nucleus Extractor

technical field

The invention relates to an instrument for extracting nuclei of brain tissue, belonging to the technical field of laboratory equipment.

Background technique

In animal experiments of rat nervous system disease models, it is often necessary to locate brain tissues, such as PVN (paraventricular nucleus of hypothalamus), hippocampus, RVLM (rostral ventrolateral medulla) or other nerve nuclei tissues, etc. Tissue itself is small in size and complex in structure, so it is extremely difficult to accurately and completely obtain nuclei of smaller brain tissue from the brain. Now the more popular method of nuclei extraction is to use a blade to roughly determine the position of the desired nuclei tissue through the rat brain atlas, and then cut and separate. This method uses materials that are easy to obtain and save, but due to the imprecise position of the nuclei, it is easy to cut during the cutting process. The nucleus is partially cut away, causing irreversible damage. In the biological laboratory, due to the different operating levels of the experimenters, the desired brain tissue nuclei are often damaged due to improper operation, which affects the subsequent experiments, which is also a major obstacle to neuroscience research. Therefore, it is a technical problem to be solved urgently by those skilled in the art to provide a brain nuclei extractor with improved accuracy and high cost performance.

SUMMARY OF THE INVENTION

Therefore, the technical problem to be solved by the present invention is the problem that the existing nuclear group extraction organization accidentally destroys the nuclear group during the operation process or cannot accurately obtain the required nuclear group, and further provides a method suitable for accurately locating and extracting the target nuclear group. brain nuclei extractor.

In order to solve the above-mentioned technical problems, a brain tissue nucleus extractor of the present invention includes: a fixing groove for holding brain tissue, the fixing groove has a scale value; an extraction device located above the fixing groove, so The extraction device includes an extraction cylinder, an extraction needle disposed under the extraction cylinder, and a piston slidably connected to the inner wall of the extraction cylinder; and a positioning device, which is fixedly connected to the extraction cylinder and drives the extraction cylinder The cylinder moves vertically and horizontally.

In the above-mentioned brain tissue nuclei extractor, the positioning device includes a fixed arm, one end of the fixed arm is fixedly connected to the extraction cylinder, and the other end is fixedly connected to the pressing member, the pressing member and the fixing groove are located. A damping member is arranged between the horizontal planes, and under the action of the damping member, the extraction cylinder moves up and down slowly under the driving of the pressing member.

In the above apparatus for extracting brain tissue nuclei, the fixed arm includes a first hinge part, a second hinge part and a third hinge part, the first hinge part and the pressing member are connected by a first fixed arm, and the first hinge part is connected to the pressing part. A hinge part and the second hinge part are connected by a second fixed arm, the first hinge part and the third hinge part are connected by a third fixed arm, and the second hinge part and the extraction cylinder are connected by a third fixed arm. Four fixed arms are connected, and the third hinge part is connected with the extraction cylinder through a fifth fixed arm.

In the above apparatus for extracting brain tissue nuclei, a clamping sleeve is formed at the end of the first fixing arm connected to the pressing member, which is suitable for being clamped and fixed with the pressing member.

In the above-mentioned brain tissue nucleus extractor, the fourth fixed arm is formed with a first half-ring ferrule at the end connected to the extraction cylinder, and the fifth fixed arm is formed with a second half-ring at the end connected with the extraction cylinder. A ferrule, the first half-annular ferrule and the second half-ring ferrule are connected and fixed by a screw connection.

In the above-mentioned brain tissue nucleus extractor, the fixing groove is arranged on the support platform, the pressing member is a sleeve structure, the pressing member is sleeved on the support frame located on the support platform, and the damping member is a sleeve a spring arranged on the support frame.

In the above apparatus for extracting brain tissue nuclei, the fixing groove is a square groove, and the fixing groove is surrounded by the support platform and four limit pieces arranged on the support platform, and the limit pieces have scales. value.

In the above apparatus for extracting nuclei of brain tissue, the limiting piece includes three fixed limiting pieces that are fixedly connected to the supporting platform, and a movable limiting piece that can move relative to the supporting platform.

In the above apparatus for extracting nuclei of brain tissue, the extraction needle includes a nipple, a needle plug and a needle head in order from top to bottom, the nipple is sealedly connected to the extraction cylinder, and the end of the needle head is provided with a needle tip.

In the above apparatus for extracting brain tissue nuclei, the upper side of the piston is integrally formed with a piston shaft and a piston handle, and the upper end of the extraction cylinder is provided with a rubber sleeve for sealingly cooperating with the piston.

The technical scheme of the present invention has the following advantages: using the brain tissue nucleus extraction apparatus with the above structure, the pretreated rat brain (both the required nucleus, cerebellum or medulla oblongata, etc. is determined before using the apparatus) is fixed in a In the fixed groove with scale, read the length and width, mark the length and width of the rat brain according to the rat brain atlas, calculate the ratio, determine the specific position of the brain tissue nuclei to be extracted, and mark it by the cross positioning method. The extraction device can be moved up and down or horizontally driven by the positioning device. The horizontal movement includes forward and backward movement and left and right movement. The experimenter can move the extraction device according to the position of the nucleus, and move the needle extractor in the instrument to the cross point. The brain tissue is pierced vertically downward, and then the extracted brain tissue is sucked into the extraction cylinder by pulling back the upper piston, so as to achieve the purpose of extracting brain tissue nuclei accurately and without injury. It is simple and convenient, with high cost performance and high precision. It is greatly improved, which is of great help to the success of subsequent experiments.

Description of drawings

In order to illustrate the specific embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the specific embodiments or the prior art. Obviously, the accompanying drawings in the following description The drawings are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative efforts.

1 is a schematic structural diagram of an embodiment of the present invention;

Description of reference numbers:

1- Needle tip, 2- Needle head, 3- Needle pin, 4- Nipple, 5- Extraction barrel, 6- Screw connection, 7- Piston, 8- Piston shaft, 9- Piston handle, 10- Fixed arm, 11- Press Pieces, 12- Damping Pieces, 13- Support Platform, 14- Fixing Slots.

Detailed ways

The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation or a specific orientation. construction and operation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

In the description of the present invention, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, unless otherwise expressly specified and limited, for example, it may be a fixed connection or a detachable connection Connection, or integral connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the internal communication of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

FIG. 1 shows the brain tissue nuclei extractor of the present invention, including: a fixing groove 14 for holding brain tissue, the fixing groove 14 has scale values; an extraction device located above the fixing groove 14, so The extraction device includes an extraction cylinder 5, an extraction needle disposed below the extraction cylinder 5, and a piston 7 slidably connected to the inner wall of the extraction cylinder 5; and a positioning device, which is fixedly connected to the extraction cylinder 5, And drive the extraction cylinder 5 to move vertically and horizontally.

Using the brain tissue nuclei extracting instrument with the above structure, the pretreated rat brain (that is, the required nuclei are determined in the cerebrum, cerebellum or medulla oblongata before using the instrument) is fixed in a fixed groove 14 with a scale, Read the length and width, mark the length and width of the rat brain according to the rat brain atlas, calculate the ratio, and determine the specific position of the brain tissue nuclei to be extracted. Move up and down or move horizontally, among which horizontal movement includes forward and backward movement and left and right movement. The experimenter can move the extraction device according to the position of the nucleus, move the needle 2 extractor in the instrument to the cross point, and pierce the brain tissue vertically downward. , and then through the pullback of the upper piston 7, the taken brain tissue is sucked into the extraction cylinder 5, so as to achieve the purpose of extracting brain tissue nuclei accurately and without injury, which is simple and convenient, cost-effective, and the accuracy is greatly improved. The success of subsequent experiments was greatly helpful.

The structure of the positioning device is not unique. As a preferred embodiment, as shown in FIG. 1 , the positioning device includes a fixed arm 10, one end of the fixed arm 10 is fixedly connected to the extraction cylinder 5, and the other end is fixed Connected to the pressing member 11 , a damping member 12 is provided between the pressing member 11 and the horizontal plane where the fixing groove 14 is located. Move slowly up and down. Since the extraction of brain tissue nuclei requires accurate positioning on the one hand, and slow suction on the other hand, the damping member 12 is provided to achieve auxiliary positioning during suction, and the suction effect is better.

More specifically, the fixing groove 14 is disposed on the supporting platform 13 , the pressing member 11 is a sleeve structure, the pressing member 11 is sleeved on the supporting frame located on the supporting platform 13 , and the damping member 12 is A spring sleeved on the support frame. The above-mentioned structure is simple, the assembly is convenient, and the operation is convenient.

The above structure realizes the vertical movement of the extraction device, and the horizontal movement of the extraction device is realized by the following specific structures:

The fixed arm 10 includes a first hinge part, a second hinge part and a third hinge part, the first hinge part and the pressing member 11 are connected by a first fixed arm, and the first hinge part is connected to the The two hinged parts are connected by a second fixed arm, the first hinged part and the third hinged part are connected by a third fixed arm, and the second hinged part and the extraction cylinder 5 are connected by a fourth fixed arm, so The third hinge part is connected with the extraction cylinder 5 through a fifth fixed arm.

Wherein, the second hinge part and the third hinge part are located on the same horizontal plane or the same vertical plane. The following takes the second hinge part and the third hinge part located on the same vertical plane as an example to illustrate the process that the positioning device drives the extraction device to move horizontally.

When the extraction device needs to move back and forth, control the second fixed arm and the third fixed arm to rotate around the first hinge part in the front and rear directions, or control the fourth fixed arm to rotate around the second hinge part in the front and rear directions, and the fifth fixed arm to rotate around the first hinge part. The third hinge portion rotates in the front-rear direction.

When the extraction device needs to move left and right, control the second fixed arm and the third fixed arm to rotate around the first hinge part up and down, or control the fourth fixed arm to rotate around the second hinge part up and down, and the fifth fixed arm Rotate up and down around the third hinge part.

More specifically, the first fixing arm is formed with a ferrule (not shown in the figure) at the end connected with the pressing member 11 , and the ferrule is clamped on the outer wall of the pressing member 11 and is suitable for fixing the first fixing arm. The arm is clamped and fixed with the pressing member 11 .

More specifically, the fourth fixed arm is formed with a first half-ring ferrule (not shown in the figure) at the end connected with the extraction cylinder 5 , and the fifth fixed arm is formed with the end connected with the extraction cylinder 5 . A second half-ring ferrule (not shown in the figure), the first half-ring ferrule and the second half-ring ferrule are connected and fixed by a threaded connection member 6 .

The structure of the above-mentioned fixing slot 14 is not unique. Preferably, the fixing slot 14 is a square slot, and the fixing slot 14 is surrounded by the supporting platform 13 and the four limiting pieces arranged on the supporting platform 13 . There are scale values on the limit piece. In other embodiments, a fixing groove that is provided independently from the supporting platform 13 may also be used, and a magnetic attraction structure may be provided at the bottom of the fixing groove to be connected to the supporting platform 13 .

The limiting piece includes three fixed limiting pieces that are fixedly connected to the support platform 13 , and a movable limiting piece that can move relative to the supporting platform 13 . The movable limit piece moves flexibly to fix different sizes of brain tissue. The limit piece of the fixing slot 14 is made of stainless steel sheet, and can have scales on it. The measuring range is 2.4cm long, 1.4cm wide, and each scale is 10mm. .

The extraction needle includes a nipple 4 , a needle plug 3 and a needle head 2 in order from top to bottom. The nipple is sealedly connected to the extraction cylinder 5 , and the end of the needle head 2 is provided with a needle tip 1 . Needle 2 is made of stainless steel, with a length of 250mm and an inner diameter of 16mm.

A piston shaft 8 and a piston handle 9 are integrally formed on the upper side of the piston 7 , and a rubber sleeve (not shown in the figure) is provided on the upper end of the extraction cylinder 5 for sealing with the piston 7 . When the piston 7 is compressed into the extraction cylinder 5, the rubber sleeve is turned outward, and when the piston 7 is pulled out of the extraction cylinder 5, the rubber sleeve is turned inward due to the friction between the cylinder wall and the rubber sleeve, isolating the internal and external air and ensuring that the gas does not flow from there. leakage.

Obviously, the above-mentioned embodiments are only examples for clear description, and are not intended to limit the implementation manner. For those of ordinary skill in the art, changes or modifications in other different forms can also be made on the basis of the above description. There is no need and cannot be exhaustive of all implementations here. And the obvious changes or changes derived from this are still within the protection scope of the present invention.

Claims (8)

1. A brain tissue nuclei extraction apparatus, comprising: a fixation groove (14) for containing brain tissue, the fixation groove (14) having a scale value thereon; the extraction device is positioned above the fixing groove (14) and comprises an extraction cylinder (5), an extraction needle arranged below the extraction cylinder (5) and a piston (7) which is connected to the inner wall of the extraction cylinder (5) in a sliding manner; the positioning device is fixedly connected with the extraction cylinder (5) and drives the extraction cylinder (5) to vertically and horizontally move; the positioning device comprises a fixing arm (10), one end of the fixing arm (10) is fixedly connected with the extraction cylinder (5), the other end of the fixing arm is fixedly connected to a pressing piece (11), a damping piece (12) is arranged between the horizontal planes of the pressing piece (11) and the fixing groove (14), and the extraction cylinder (5) is driven by the pressing piece (11) to move up and down slowly under the action of the damping piece (12); the fixing arm (10) comprises a first hinged portion, a second hinged portion and a third hinged portion, the first hinged portion is connected with the pressing piece (11) through a first fixing arm, the first hinged portion is connected with the second hinged portion through a second fixing arm, the first hinged portion is connected with the third hinged portion through a third fixing arm, the second hinged portion is connected with the extracting cylinder (5) through a fourth fixing arm, and the third hinged portion is connected with the extracting cylinder (5) through a fifth fixing arm.

2. The apparatus according to claim 1, wherein the first fixing arm is formed with a ferrule at an end connected to the pressing member (11) and adapted to be snap-fitted to the pressing member (11).

3. The brain tissue nuclei extraction instrument according to claim 1, wherein the fourth fixed arm is formed with a first semi-annular ferrule at one end connected with the extraction cylinder (5), the fifth fixed arm is formed with a second semi-annular ferrule at one end connected with the extraction cylinder (5), and the first semi-annular ferrule and the second semi-annular ferrule are fixedly connected through a threaded connector (6).

4. The brain tissue nuclei extraction instrument of claim 1, wherein the fixing groove (14) is disposed on a supporting platform (13), the pressing member (11) is a sleeve structure, the pressing member (11) is sleeved on a supporting frame disposed on the supporting platform (13), and the damping member (12) is a spring sleeved on the supporting frame.

5. The brain tissue nuclei extraction apparatus of claim 4, wherein the fixing groove (14) is a square groove, the fixing groove (14) is defined by the supporting platform (13) and four limiting pieces arranged on the supporting platform (13), and the limiting pieces have scale values.

6. The apparatus according to claim 5, wherein the limiting plate comprises three fixed limiting plates fixedly connected to the supporting platform (13) and a movable limiting plate movable relative to the supporting platform (13).

7. The brain tissue nuclei extraction instrument of claim 1, wherein the extraction needle comprises a nipple (4), a pintle (3) and a needle head (2) from top to bottom in sequence, the nipple is hermetically connected with the extraction cylinder (5), and the end of the needle head (2) is provided with a needle point (1).

8. The brain tissue nuclei extraction instrument according to claim 1, wherein the piston shaft (8) and the piston handle (9) are integrally formed on the upper side of the piston (7), and the upper end of the extraction cylinder (5) is provided with a rubber sleeve for sealing engagement with the piston (7).

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