CN106259067B - A kind of selection of high temperature resistant stichopus japonicus strain - Google Patents
A kind of selection of high temperature resistant stichopus japonicus strain Download PDFInfo
- Publication number
- CN106259067B CN106259067B CN201610634181.2A CN201610634181A CN106259067B CN 106259067 B CN106259067 B CN 106259067B CN 201610634181 A CN201610634181 A CN 201610634181A CN 106259067 B CN106259067 B CN 106259067B
- Authority
- CN
- China
- Prior art keywords
- high temperature
- stichopus japonicus
- family
- breeding
- selection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
Abstract
The invention discloses a kind of selections of high temperature resistant stichopus japonicus strain, belong to aquatic products genetic breeding field.Survival stichopus japonicus is as parent after the present invention is eliminated by selection different geographical summer natural high-temperature, breeding building family after sexal maturity, 28 DEG C of heat shocks are carried out in each family auricularia stage, retain 20% family by survival rate and abnormal rate, when then retaining family mixed breed to individual average weight > 50g, carry out 32 DEG C of progress high temperature stress, removal death and impaired individuals, retain≤25% individual and continues cultivation to sexal maturity, it is carried out continuously heat shock selection and breeding three generations with principle of identity, obtains the stichopus japonicus new lines with high temperature resistant character.Stichopus japonicus original parent of the present invention more meets the patience characteristic of practical breeding environment；The screening of larval stage family high temperature acclimation and cultivation phase high temperature stress screening, family selective breeding improve efficiency of selection in conjunction with unexpected mass incident；Continuous three generations's breeding, consolidates high temperature resistant character, breeding prospect with good economic efficiency and wide.
Description
Technical field
The present invention relates to a kind of selections of high temperature resistant stichopus japonicus strain, belong to aquatic products genetic breeding field.
Background technique
Apostichopus japonicus culture is the important sea-farming industry of northern China.In recent years as people are to the understanding of stichopus japonicus nutritive value,
The market demand persistently overheating of stichopus japonicus product, the cultivation scale of stichopus japonicus are expanded rapidly with the increase of the market demand, are cultivated at present
Scale has reached millions of mus, and nearly 20,000,000,000 yuan of annual value of production, be the highest type of China's sea-farming single variety output value.However, with
The variation of global climate, while global average temperature rises, extreme weather such as summer high temperature etc. is also in many areas in the whole world
Domain more frequently occurs, and China's summer lasting hot weather caused Stichopus japonicus pond water temperature high in recent years, and the duration is long, part
Regional pond superficial water high temperature causes to seriously affect up to 32 DEG C or more to pond culture stichopus japonicus, the rotten death of a large amount of stichopus japonicus, only
There are a small number of individual survivals.It is reported that the Along The Coast of Shandong Province culturing sea cucumbers of summer in 2013 are generally by continuous high temperature disaster, Dongying is tried to gain
The ground such as flat, Laizhou, Rushan have resulted in Large Scale Death disaster, and the billions of members of direct economic loss, to apostichopus japonicus culture, producer is brought
Heavy blow has seriously affected the sound development of summer apostichopus japonicus culture industry.In addition, cultivation is moved to resistance in the scale south of stichopus japonicus
The juvenile stichopus of high temperature character proposes demand.The selection for inventing a kind of high temperature resistant stichopus japonicus, cultivates stichopus japonicus resistant to high temperature
New varieties are the urgent needs of stichopus japonicus efficient health cultivation.
Summary of the invention
The purpose of the present invention is to provide a kind of selections of high temperature resistant stichopus japonicus strain, are suitable for stichopus japonicus breeding and seed
It cultivates, improves the ability of stichopus japonicus reply hot environment, reduce loss of the summer high temperature to apostichopus japonicus culture, there is good economic effect
Benefit.
For achieving the above object, a kind of selection of high temperature resistant phase stichopus japonicus strain, it is characterised in that:
The following steps are included:
1) survival stichopus japonicus each 100 are used as parent after the summer high temperature of selection different geographical is eliminated, and cultivation to sexal maturity is close
This breeding constructs family, each family cultivating water box isolation culture；
2) each family stichopus japonicus is developed to the auricularia stage, takes the young to set heat shock in 27 DEG C of seawater and tames, experiment stichopus japonicus children
Body is cultivated to young ginseng, and survival rate and abnormal rate are counted；
3) it according to statistics survival rate and abnormal rate, eliminates that survival rate is low and the high family of abnormal rate by sorting, retains 20%
System, retains family mixing, and Routine Management is cultivated；
4) when retaining family mixed breed to individual average weight > 50g, big individual >=500 progress heat resistances are taken at random
Experiment, control breeding water body water temperature rise to 32 DEG C and are coerced, and choose dead individuals, flowing water culture at any time, it is ensured that water temperature and ring
The maintenance in border；
5) experiment terminates the number that record finally survives individual, eliminates dead and impaired individuals, continues conventional cultivation to property
It is mature；
6) sexal maturity stichopus japonicus >=100 are continued to construct family by step 1-5 principle, carry out the screening of larval stage family high temperature
With cultivation phase high temperature unexpected mass incident, continuous choice breeding three generations, until stichopus japonicus new lines of the acquisition with high temperature resistant character.
Further, stichopus japonicus original parent described in step 1) uses Yantai, Qingdao, three ground pond culture stichopus japonicus summer of Dongying
Survive stichopus japonicus after natural high-temperature is superseded, guarantees its parent's genetic diversity and with the patience characteristic for meeting practical breeding environment.
Further, family described in step 1) is configured to establish 60 or more full sibs with the principle of 1-3 hero ginseng with 1 female ginseng
Family and family half sibs.
Further, all familys described in step 2 be developed to auricularia heat shock domestication the time be 120min.
Further, cultivation 32 DEG C of high temperature stress temperature control operation processes of phase described in step 4) are from normal water temperature (18
± 1) slow after DEG C rising to 25 DEG C by the speed that every 12h increases by 1 DEG C, then by rising to 32 DEG C, 32 DEG C of maintenances 72h per increasing by 1 DEG C for 24 hours
Slow cooling.
Further, step 5) removal is dead and impaired individuals ,≤25% individual of reservation continue to cultivate.
Further, family selective breeding and unexpected mass incident strategy can be also used in step 6) simultaneously, is carried out continuously three generations's breeding,
Obtain high temperature resistant character stichopus japonicus.
The present invention has the advantages that 1, stichopus japonicus original parent is survived after being eliminated using pond culture stichopus japonicus summer natural high-temperature
Stichopus japonicus, individual have the patience characteristic for more meeting practical breeding environment；2, family selective breeding is in conjunction with unexpected mass incident, larval stage man
It is high temperature acclimation screening and cultivation phase high temperature stress screening, improves efficiency of selection；3, continuous three generations's breeding consolidates heat-resisting quantity
Shape moves cultivation and provides fine germplasm resources, improves apostichopus japonicus culture economic benefit for summer high temperature apostichopus japonicus culture and stichopus japonicus south.
Specific embodiment
Below in conjunction with detailed description of the invention by specific embodiments:
1) stichopus japonicus parent is living individuals after the Yantai, Qingdao, three ground pond culture summer high temperature of Dongying collected are eliminated, pond
32 DEG C of pool water temperature or more high temperature continue 5 days, eliminate survival rate < 0.05%.Each 100 of three groups are used as parent, cultivation to property at
Ripe, the mode of artificial cold water decorporation carries out parent participation breeding, establishes 60 or more full sibs men with the principle of 1-3 hero ginseng with 1 female ginseng
System and family half sibs actually establish family 72, each family cultivating water box isolation culture；
2) each family stichopus japonicus is developed to the auricularia stage, takes each family stichopus japonicus young 1L(density 0.5 with glass beaker
Inds/mL), it is placed in and presets in 27 DEG C of thermostat water baths, during which heat shock 120min uses microthermometer every 5min
(accuracy ± 0.2 DEG C), which measures and heats water adjustment, keeps water temperature.Then by stichopus japonicus be transferred to renewal cultivation at 17 DEG C cultivate to
Young ginseng observes stichopus japonicus animation, counts the death rate and abnormal rate；
3) count survival rate and abnormal rate, non-heat shock stichopus japonicus auricularia survival rate 68% or so, after heat shock 72 familys at
Motility rate is between 24.5%-47%, abnormal rate 8%-11.5%.By the high family of the superseded death rate height of sequence and abnormal rate, retain 20%
Family totally 14, survival rate>40%, abnormal rate<10%.Retain family mixing, Routine Management is cultivated；
4) when each stichopus japonicus family mixed breed is to individual average weight > 50g, healthy and strong big individual >=500 progress are taken at random
Heat resistance experiment.Breeding water body water temperature is controlled with controllable constant-temperature heating rod, increases by 1 DEG C from normal water temperature (18 ± 1) DEG C by every 12h
Speed rise to 25 DEG C after, then by per for 24 hours increase by 1 DEG C rise to 32 DEG C, 32 DEG C of maintenance 72h, slow cooling.Choose dead at any time
Body, flowing water culture, it is ensured that the maintenance of water benign environment；
5) slow cooling after high temperature stress restores room temperature culture, and record finally survives the number of individual, practical to survive
122, survival rate 24.4% retains individual and continues conventional cultivation to sexal maturity；
6) sexal maturity stichopus japonicus >=100 continue to carry out parent participation breeding by step 1-5 principle, construct family, carry out children in succession
The screening of body phase family high temperature acclimation and cultivation phase high temperature stress screening, continuous breeding three generations；
7) high temperature resistant stichopus japonicus being obtained as parent participation and breeding filial generation, this is the stichopus japonicus new lines with high temperature resistant character.
Claims (6)
1. a kind of selection of high temperature resistant stichopus japonicus strain, which comprises the following steps:
1) survival stichopus japonicus each 100 are used as parent, cultivation to sexal maturity, Qin Benfan after the summer high temperature of selection different geographical is eliminated
Educate building family, each family cultivating water box isolation culture；
2) each family stichopus japonicus is developed to the auricularia stage, takes the young to set heat shock in 27 DEG C of seawater and tames, stichopus japonicus larval culture is extremely
Young ginseng counts survival rate and abnormal rate；
3) it according to statistics survival rate and abnormal rate, eliminates that survival rate is low and the high family of abnormal rate by sorting, retains 20% family,
Retain family mixing, Routine Management is cultivated；
4) when retaining family mixed breed to individual average weight > 50g, big individual >=500 progress heat resistance experiments are taken at random,
Control breeding water body water temperature rises to 32 DEG C and is coerced, and chooses dead individuals, flowing water culture at any time, it is ensured that the dimension of water benign environment
It holds；
5) experiment terminates the number that record finally survives individual, eliminates dead and impaired individuals, continues conventional cultivation to sexal maturity；
6) sexal maturity stichopus japonicus >=100 are continued to construct family by step 1-5 principle, are carried out the screening of larval stage family high temperature and are supported
Phase high temperature unexpected mass incident, continuous choice breeding three generations are grown, until obtaining the stichopus japonicus new lines with high temperature resistant character.
2. the selection of high temperature resistant stichopus japonicus strain according to claim 1, it is characterised in that: stichopus japonicus described in step 1) is former
Beginning parent is survived stichopus japonicus after being eliminated using Yantai, Qingdao, three ground pond culture stichopus japonicus summer natural high-temperature of Dongying, guarantees its parent
Genetic diversity and with the patience characteristic for meeting practical breeding environment.
3. the selection of high temperature resistant stichopus japonicus strain according to claim 1, it is characterised in that: construct house described in step 1)
System establishes 60 or more family full-sibs and family half sibs for the principle joined with 1 female ginseng with 1-3 hero.
4. the selection of high temperature resistant stichopus japonicus strain according to claim 1, it is characterised in that: all families described in step 2
System is developed to auricularia heat shock and tames the time as 120min.
5. the selection of high temperature resistant stichopus japonicus strain according to claim 1, it is characterised in that: cultivate the phase described in step 4)
32 DEG C of high temperature stress temperature control operation processes are to rise to 25 DEG C from normal water temperature (18 ± 1) DEG C by the speed that every 12h increases by 1 DEG C
Afterwards, then by per 1 DEG C of increase for 24 hours 32 DEG C, 32 DEG C of maintenance 72h are risen to, slow cooling.
6. the selection of high temperature resistant stichopus japonicus strain according to claim 1, it is characterised in that: step 5) removal it is dead and
Impaired individuals retain≤25% individual and continue to cultivate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610634181.2A CN106259067B (en) | 2016-08-05 | 2016-08-05 | A kind of selection of high temperature resistant stichopus japonicus strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610634181.2A CN106259067B (en) | 2016-08-05 | 2016-08-05 | A kind of selection of high temperature resistant stichopus japonicus strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106259067A CN106259067A (en) | 2017-01-04 |
| CN106259067B true CN106259067B (en) | 2019-03-01 |
Family
ID=57665129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610634181.2A Active CN106259067B (en) | 2016-08-05 | 2016-08-05 | A kind of selection of high temperature resistant stichopus japonicus strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106259067B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107006409B (en) * | 2017-04-14 | 2019-03-08 | 山东省海洋生物研究院 | A kind of selection of the degeneration-resistant stichopus japonicus new lines of fast-growing |
| CN115948577B (en) * | 2023-01-09 | 2023-08-22 | 山东省渔业发展和资源养护总站 | Breeding method of high-temperature-resistant sea cucumber |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720685A (en) * | 2008-10-30 | 2010-06-09 | 大连水产学院 | High strength selecting and cultivating method of excellent stichopus japonicus seeds |
| CN102144587A (en) * | 2011-03-30 | 2011-08-10 | 浙江省海洋水产研究所 | Method for breeding high-temperature-resistant Paralichthys olivaceus new varieties |
| CN104365516A (en) * | 2014-11-25 | 2015-02-25 | 山东省海洋资源与环境研究院 | Method for breeding stichopus japonicus through shortening of aestivation period |
| KR20150060625A (en) * | 2015-04-23 | 2015-06-03 | 장철중 | The construction methode of sea cucumber culture farms |
| CN105766707A (en) * | 2016-02-01 | 2016-07-20 | 大连海洋大学 | Cultivation method for high temperature resistance sea urchins |
-
2016
- 2016-08-05 CN CN201610634181.2A patent/CN106259067B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720685A (en) * | 2008-10-30 | 2010-06-09 | 大连水产学院 | High strength selecting and cultivating method of excellent stichopus japonicus seeds |
| CN102144587A (en) * | 2011-03-30 | 2011-08-10 | 浙江省海洋水产研究所 | Method for breeding high-temperature-resistant Paralichthys olivaceus new varieties |
| CN104365516A (en) * | 2014-11-25 | 2015-02-25 | 山东省海洋资源与环境研究院 | Method for breeding stichopus japonicus through shortening of aestivation period |
| KR20150060625A (en) * | 2015-04-23 | 2015-06-03 | 장철중 | The construction methode of sea cucumber culture farms |
| CN105766707A (en) * | 2016-02-01 | 2016-07-20 | 大连海洋大学 | Cultivation method for high temperature resistance sea urchins |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106259067A (en) | 2017-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107637551B (en) | Method for breeding new strain of northern low-temperature-resistant crassostrea sikamea | |
| CN103168751B (en) | Expanding propagation method for aphidius gifuensis ashmeads | |
| CN106508760B (en) | A kind of selection of bay scallop high temperature resistant new lines | |
| CN103141424B (en) | Rapid screening method for high-temperature-resistant mizuhopecten yessoensis breeding pedigrees | |
| CN103548722A (en) | Hippocampus kelloggi ecological breeding method | |
| CN103222425A (en) | Efficient and rapid propagation technology suitable for southern highbush blueberry | |
| CN101473791B (en) | Method for breeding spring cabbage stalk and maintaining springness of inbred line | |
| CN106259067B (en) | A kind of selection of high temperature resistant stichopus japonicus strain | |
| CN105340755A (en) | Method for continuously culturing high frequency regeneration plants through cereal crop single plant source microspores | |
| CN105850747A (en) | Rapid propagation method for tissue of succulent sedum rubrotinctum and sedum rubrotinctum cultured with method | |
| CN102696483A (en) | Method for quickly propagating lilium fargesii | |
| CN102511450A (en) | Artificial domestication method for wild leeches | |
| CN100394845C (en) | In-bottle production method of detoxified small seed ball of east lily | |
| CN104488723A (en) | Tissue-culture and rapid-propagation method of epimedium koreanum nakai | |
| CN100574614C (en) | A kind of calla lily germplasm resources in vitro conservation method | |
| CN205658206U (en) | VHD batch production nian fish farming systems | |
| CN107125136A (en) | A kind of Camellia nitidissima tissue culture mating system | |
| CN101904302B (en) | Method for somatic cell embryogeny and plant regeneration of medicinal plant schisandga chinensis baill | |
| CN106258903A (en) | A kind of method of large-scale production Pseudobulbus Bletillae (Rhizoma Bletillae) seedling | |
| CN106416972A (en) | Method for quickly cultivating bletilla striata seedlings | |
| CN103798168B (en) | One is applicable to the indoor extensive artificial breeding new method of the South China coastal salinity gradual change type Kumamoto oyster | |
| CN101012448A (en) | Method of screening leymus chinensis salt-tolerant somatic mutation | |
| CN105210863A (en) | A kind of tissue culture and rapid propagation method of rhizoma Gastrodiae | |
| CN104365516B (en) | A kind of breeding method of short estivation period stichopus japonicus | |
| CN106305421B (en) | A method of cultivating salt tolerant switchgrass |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |