CN106236601A - Vital preservation method for the stem cell factor of beauty and skin care - Google Patents
Vital preservation method for the stem cell factor of beauty and skin care Download PDFInfo
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- CN106236601A CN106236601A CN201610630544.5A CN201610630544A CN106236601A CN 106236601 A CN106236601 A CN 106236601A CN 201610630544 A CN201610630544 A CN 201610630544A CN 106236601 A CN106236601 A CN 106236601A
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- stem cell
- factor
- liposome
- beauty
- skin care
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses the vital preservation method of the stem cell factor for beauty and skin care, complete the vital preservation to the stem cell factor for beauty and skin care including raw-material selection, the preparation of liposome and lyophilizing technique；Specifically comprise the following steps that the selection of the Stem Cell Activity factor: select the purity Stem Cell Activity factor more than or equal to 99%；The preparation of liposome: include the selection of hydrous solvent, the determination of medicine fat ratio and ultrasonic coating technique；Lyophilizing technique: prepared liposome is dissolved in cell culture supernatant by certain concentration, and add a certain proportion of excipient, in freeze dryer, evacuation makes liquid sublimation, forms flour.The present invention is by using preparation and the freeze drying technology of liposome, effectively extending the Stem Cell Activity factor vital preservation time limit in a liquid state, the Stem Cell Activity factor is applied to cosmetic field by the present invention, it is achieved biological cosmetics effect, directly acting on Skin Cell aspect, using effect is good.
Description
Technical field
The present invention relates to the vital preservation method field of stem cell factor, be specifically related to a kind of for
The vital preservation method of the stem cell factor of beauty and skin care.
Background technology
Stem cell is the pluripotent cell that a class has the of self-replication capacity.Under certain condition, it can be divided into many
Plant functioning cell.It is divided into embryonic stem cell and adult stem cell according to the stage of development residing for stem cell.Sending out according to stem cell
Educate potential to be divided three classes: myeloid-lymphoid stem cell, pluripotent stem cell and unipotent stem cell.Stem cell is cells of origin.Stem cell has
There is the ability that self renewal replicates, it is possible to produce well differentiated functioning cell.
The aging of human body, the appearance of wrinkle, tracing sth. to its source is the most all aging and the minimizing of cell.And the aging of cell
Then caused by stem cell is aging with reducing.Aging its propagation and the ability of differentiation of seriously reducing of stem cell group, newly
Raw cell supplements deficiency, and senile cell can not be substituted in time, and each systemic-function of whole body declines, and allows people the most always go.And
Your skin, also cannot upgrade in time because of the aging of skin progenitor cell, and old and feeble skin can not get repairing, so, you have
Wrinkle, loses youth appearance.
Research simultaneously shows that stem cell factor can quickly activate the stem cell of dormancy and promote it to grow, simultaneously can
With microenvironment in regulation body, provide favourable growth conditions for stem cell.Somatomedin mostly is the peptide hormone of broad sense, has epidermis
Somatomedin, fibroblast growth factor (FGF), platelet source multiplicaiton factor etc..There are some researches show such somatomedin in beauty treatment side
Surface function is powerful: 1) damaged skin, sensitive skin, trauma skin and reconstruction property skin are had good reparation, nursing is made
With；2) whitening, light speckle；3) shorten the wound healing time and reduce cicatrization；4) regeneration vessel, promotes that collagen protein closes
Become, slow down aging etc..
But when cream ointment or the aqueous emulsion of tradition beauty and skin care, the vital preservation time of stem cell factor
The shortest, do not reach the effect that beauty and skin care is anti-ageing, which greatly limits the effect in terms of the beauty and skin care of the Stem Cell Activity factor.
And the present invention adds freeze drying technology by liposome technology, retaining stem cell factor product beautifying and antisenility effect
The most greatly enhance the stem cell factor vital preservation phase in the product simultaneously, and the method is suitable to extensive raw
Produce, serve beauty treatment cause for stem cell factor and provide a new road.
Summary of the invention
Present invention aims to the deficiencies in the prior art, now provide one can retain stem cell factor and produce
Product beautifying and antisenility effect, be also greatly enhanced simultaneously the stem cell factor vital preservation phase in the product for beauty and skin care
The vital preservation method of stem cell factor.
For solving above-mentioned technical problem, the technical solution used in the present invention is: for the stem cell factor of beauty and skin care
Vital preservation method, its innovative point is: include that raw-material selection, the preparation of liposome and lyophilizing technique complete for U.S.
Hold the vital preservation of the stem cell factor of skin protection；Specifically comprise the following steps that
(1) selection of the Stem Cell Activity factor: select the purity Stem Cell Activity factor more than or equal to 99%；
(2) preparation of liposome: include the selection of hydrous solvent, the determination of medicine fat ratio and ultrasonic coating technique；
(3) lyophilizing technique: prepared liposome is dissolved in cell culture supernatant by certain concentration, and add a certain proportion of
Excipient, in freeze dryer, evacuation makes liquid sublimation, forms flour.
Further, the specifically comprising the following steps that of the preparation of described step (2) liposome
A. the selection of hydrous solvent: select PH be 7.4 PBS solvent, PH be 7.0 secondary aqueous solvent, PH be the D-of 7.4
Nacl, concentration that D-Hanks that Hanks, PH are 9.5, concentration are 0.9% are the one in the glucose of 5%；
B. the determination of medicine fat ratio: the described Stem Cell Activity factor is 1:1 with the mass ratio of liposome；
The most ultrasonic coating technique: lecithin, cholesterol and stem cell factor are dissolved in hydrous solvent, rotates after mix homogeneously
Evaporation, removes hydrous solvent, is processed through water bath sonicator by remaining solution again, obtains liposome after being centrifuged.
Further, the addition 22-27% of the total volume of described excipient, described lyophilized powder is dissolved in essence
Factor concentration ensures at 5 μ g/ml.
Further, lecithin, the mass ratio of cholesterol in described ultrasonic coating technique step are 3:1, described rotation
The rotating speed of evaporation is 120-160r/min, and evaporating temperature is 33-39 DEG C.
Further, the intensity of the water bath sonicator in described ultrasonic coating technique step is 30%, and each ultrasonic time is
30s is spaced 5s, and total water bath sonicator time is 4min, and described centrifugal speed is 2900-3100r/min, and described centrifugation time is
4-6min。
Beneficial effects of the present invention is as follows:
(1) present invention is by using preparation and the freeze drying technology of liposome, effectively extends the Stem Cell Activity factor in liquid condition
Under the vital preservation time limit.
(2) the Stem Cell Activity factor is applied to cosmetic field by the present invention, it is achieved biological cosmetics effect, directly acts on
In Skin Cell aspect, using effect is good.
(3) present invention is by quoting the highly purified Stem Cell Activity factor, it is achieved remove old and feeble apoptotic cell, activated sleep
Skin progenitor cell, constantly break up regenerative cell.
Accompanying drawing explanation
Fig. 1 is the water bath sonicator grain-size graph of the present invention；
Fig. 2 is the Probe Ultrasonic Searching grain-size graph of the present invention；
Fig. 3 is 0.01M PH=7.4PBS hydrous solvent grain-size graph；
Fig. 4 is secondary water hydrous solvent grain-size graph；
Fig. 5 is 0.01M PH=7.4D-Hanks hydrous solvent grain-size graph；
Fig. 6 is 0.005mol/L PH=9.5D-Hanks hydrous solvent grain-size graph；
Fig. 7 is 0.9%Nacl hydrous solvent grain-size graph；
Fig. 8 is 5% G/W bonding solvent grain-size graph.
Detailed description of the invention
By particular specific embodiment, embodiments of the present invention being described below, those skilled in the art can be by this explanation
Content disclosed by book understands other advantages and effect of the present invention easily.
Embodiment 1
For the vital preservation method of the stem cell factor of beauty and skin care, including raw-material selection, the preparation of liposome with freeze
Dry technique completes the vital preservation to the stem cell factor for beauty and skin care；Specifically comprise the following steps that
(1) selection of the Stem Cell Activity factor: select the purity Stem Cell Activity factor more than or equal to 99%；
(2) preparation of liposome: including the selection of hydrous solvent, the determination of medicine fat ratio and ultrasonic coating technique, concrete steps are such as
Under:
A. the selection of hydrous solvent: select PH be 7.4 PBS solvent, PH be 7.0 secondary aqueous solvent, PH be the D-of 7.4
Nacl, concentration that D-Hanks that Hanks, PH are 9.5, concentration are 0.9% are the one in the glucose of 5%；
B. the determination of medicine fat ratio: the Stem Cell Activity factor is 1:2 with the mass ratio of liposome；
The most ultrasonic coating technique: lecithin, cholesterol and stem cell factor are dissolved in hydrous solvent, rotates after mix homogeneously
Evaporation, removes hydrous solvent, is processed through water bath sonicator by remaining solution again, obtains liposome after being centrifuged, and lecithin, gallbladder are solid
The mass ratio of alcohol is 3:1, and the rotating speed of rotary evaporation is 120r/min, and evaporating temperature is 33 DEG C, and the intensity of water bath sonicator is 30%,
Ultrasonic time is that 30s is spaced 5s every time, and total water bath sonicator time is 4min, and centrifugal speed is 2900r/min, and centrifugation time is
4min；
(3) lyophilizing technique: prepared liposome is dissolved in cell culture supernatant by certain concentration, and add a certain proportion of
Excipient, in freeze dryer, evacuation makes liquid sublimation, forms flour, the addition of the total volume 22% of excipient,
The factor concentration that lyophilized powder is dissolved in essence ensures at 5 μ g/ml.
Embodiment 2
For the vital preservation method of the stem cell factor of beauty and skin care, including raw-material selection, the preparation of liposome with freeze
Dry technique completes the vital preservation to the stem cell factor for beauty and skin care；Specifically comprise the following steps that
(1) selection of the Stem Cell Activity factor: select the purity Stem Cell Activity factor more than or equal to 99%；
(2) preparation of liposome: including the selection of hydrous solvent, the determination of medicine fat ratio and ultrasonic coating technique, concrete steps are such as
Under:
A. the selection of hydrous solvent: select PH be 7.4 PBS solvent, PH be 7.0 secondary aqueous solvent, PH be the D-of 7.4
Nacl, concentration that D-Hanks that Hanks, PH are 9.5, concentration are 0.9% are the one in the glucose of 5%；
B. the determination of medicine fat ratio: the Stem Cell Activity factor is 1:1 with the mass ratio of liposome；
The most ultrasonic coating technique: lecithin, cholesterol and stem cell factor are dissolved in hydrous solvent, rotates after mix homogeneously
Evaporation, removes hydrous solvent, is processed through water bath sonicator by remaining solution again, obtains liposome after being centrifuged, and lecithin, gallbladder are solid
The mass ratio of alcohol is 3:1, and the rotating speed of rotary evaporation is 160r/min, and evaporating temperature is 39 DEG C, and the intensity of water bath sonicator is 30%,
Ultrasonic time is that 30s is spaced 5s every time, and total water bath sonicator time is 4min, and centrifugal speed is 3100r/min, and centrifugation time is
6min；
(3) lyophilizing technique: prepared liposome is dissolved in cell culture supernatant by certain concentration, and add a certain proportion of
Excipient, in freeze dryer, evacuation makes liquid sublimation, forms flour, the addition of the total volume 27% of excipient,
The factor concentration that lyophilized powder is dissolved in essence ensures at 5 μ g/ml.
Embodiment 3
For the vital preservation method of the stem cell factor of beauty and skin care, including raw-material selection, the preparation of liposome with freeze
Dry technique completes the vital preservation to the stem cell factor for beauty and skin care；Specifically comprise the following steps that
(1) selection of the Stem Cell Activity factor: select the purity Stem Cell Activity factor more than or equal to 99%；
(2) preparation of liposome: including the selection of hydrous solvent, the determination of medicine fat ratio and ultrasonic coating technique, concrete steps are such as
Under:
A. the selection of hydrous solvent: select PH be 7.4 PBS solvent, PH be 7.0 secondary aqueous solvent, PH be the D-of 7.4
Nacl, concentration that D-Hanks that Hanks, PH are 9.5, concentration are 0.9% are the one in the glucose of 5%；
B. the determination of medicine fat ratio: the Stem Cell Activity factor is 1:1 with the mass ratio of liposome；
The most ultrasonic coating technique: lecithin, cholesterol and stem cell factor are dissolved in hydrous solvent, rotates after mix homogeneously
Evaporation, removes hydrous solvent, is processed through water bath sonicator by remaining solution again, obtains liposome after being centrifuged, and lecithin, gallbladder are solid
The mass ratio of alcohol is 3:1, and the rotating speed of rotary evaporation is 140r/min, and evaporating temperature is 36 DEG C, and the intensity of water bath sonicator is 30%,
Ultrasonic time is that 30s is spaced 5s every time, and total water bath sonicator time is 4min, and centrifugal speed is 3000r/min, and centrifugation time is
5min；
(3) lyophilizing technique: prepared liposome is dissolved in cell culture supernatant by certain concentration, and add a certain proportion of
Excipient, in freeze dryer, evacuation makes liquid sublimation, forms flour, the addition of the total volume 25% of excipient,
The factor concentration that lyophilized powder is dissolved in essence ensures at 5 μ g/ml.
Ultrasonic power can select water bath sonicator and Probe Ultrasonic Searching, by the envelop rate of liposome and the test of particle diameter,
Test result see table:
Nano particle size instrument is used to measure the particle diameter of the PDGF-L that two kinds of ultrasonic methods prepare, the scattergram of particle diameter such as Fig. 1 and Fig. 2 institute
Show.
Considering from envelop rate and particle diameter, Probe Ultrasonic Searching method is prepared liposome and is had more preferable effect.
The screening of hydrous solvent kind: examination following six kinds of hydrous solvent envelop rate, drug loading and particle diameter to liposome
Impact, result see table.
Use nano particle size instrument to measure different hydrous solvent and prepare the particle diameter of PDGF-L, grain size distribution as shown in figures 3-8,
Shown in above-mentioned chart, selecting secondary water is that to prepare liposome be optimum selection to solvent.
The present invention, by using preparation and the freeze drying technology of liposome, effectively extends the Stem Cell Activity factor in liquid condition
Under the vital preservation time limit, the Stem Cell Activity factor is applied to cosmetic field by the present invention, it is achieved biological cosmetics effect, directly
Connecing and act on Skin Cell aspect, using effect is good, and the present invention is by quoting the highly purified Stem Cell Activity factor, it is achieved remove
Old and feeble apoptotic cell, the skin progenitor cell of activated sleep, constantly breaks up regenerative cell.
Above-described embodiment is presently preferred embodiments of the present invention, is not the restriction to technical solution of the present invention, as long as
The technical scheme that can realize on the basis of above-described embodiment without creative work, is regarded as falling into patent of the present invention
Rights protection in the range of.
Claims (5)
1. it is used for the vital preservation method of the stem cell factor of beauty and skin care, it is characterised in that: include raw-material selection, lipid
Preparation and the lyophilizing technique of body complete the vital preservation to the stem cell factor for beauty and skin care；Specifically comprise the following steps that
The selection of the Stem Cell Activity factor: select the purity Stem Cell Activity factor more than or equal to 99%；
The preparation of liposome: include the selection of hydrous solvent, the determination of medicine fat ratio and ultrasonic coating technique；
Lyophilizing technique: prepared liposome is dissolved in cell culture supernatant by certain concentration, and add a certain proportion of tax
Shape agent, in freeze dryer, evacuation makes liquid sublimation, forms flour.
The vital preservation method of the stem cell factor for beauty and skin care the most according to claim 1, it is characterised in that: institute
State the specifically comprising the following steps that of preparation of step (2) liposome
The selection of hydrous solvent: select PH be 7.4 PBS solvent, PH be 7.0 secondary aqueous solvent, PH be 7.4 D-Hanks,
Nacl, concentration that D-Hanks that PH is 9.5, concentration are 0.9% are the one in the glucose of 5%；
The determination of medicine fat ratio: the described Stem Cell Activity factor is 1:1 with the mass ratio of liposome；
Ultrasonic coating technique: lecithin, cholesterol and stem cell factor are dissolved in hydrous solvent, rotates after mix homogeneously and steams
Send out, remove hydrous solvent, remaining solution is processed through water bath sonicator again, after being centrifuged, obtain liposome.
The vital preservation method of the stem cell factor for beauty and skin care the most according to claim 1, it is characterised in that: institute
Stating the addition 22-27% of the total volume of excipient, the factor concentration that described lyophilized powder is dissolved in essence ensures at 5 μ g/
ml。
The vital preservation method of the stem cell factor for beauty and skin care the most according to claim 1, it is characterised in that: institute
State the lecithin in ultrasonic coating technique step, the mass ratio of cholesterol is 3:1, and the rotating speed of described rotary evaporation is 120-
160r/min, evaporating temperature is 33-39 DEG C.
The vital preservation method of the stem cell factor for beauty and skin care the most according to claim 1, it is characterised in that: institute
The intensity stating the water bath sonicator in ultrasonic coating technique step is 30%, and each ultrasonic time is that 30s is spaced 5s, and total water-bath surpasses
The sound time is 4min, and described centrifugal speed is 2900-3100r/min, and described centrifugation time is 4-6min.
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| CN201610630544.5A CN106236601B (en) | 2016-08-04 | 2016-08-04 | The vital preservation method of stem cell factor for beauty and skin care |
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| CN106236601A true CN106236601A (en) | 2016-12-21 |
| CN106236601B CN106236601B (en) | 2019-04-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109394563A (en) * | 2018-11-06 | 2019-03-01 | 福建省海西细胞生物工程有限公司 | A kind of preparation method of stem cell media liposome and the application in skin care item |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101683521A (en) * | 2008-09-27 | 2010-03-31 | 深圳市海王英特龙生物技术股份有限公司 | Human eryth ropoietin liposome |
| CN102512370A (en) * | 2011-12-28 | 2012-06-27 | 广州赛莱拉生物科技有限公司 | HSCGF liposome as well as preparation and application thereof |
| CN103751228A (en) * | 2014-01-27 | 2014-04-30 | 贵州神奇药物研究院 | Medicine for treating Alzheimer disease and preparation method thereof |
-
2016
- 2016-08-04 CN CN201610630544.5A patent/CN106236601B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101683521A (en) * | 2008-09-27 | 2010-03-31 | 深圳市海王英特龙生物技术股份有限公司 | Human eryth ropoietin liposome |
| CN102512370A (en) * | 2011-12-28 | 2012-06-27 | 广州赛莱拉生物科技有限公司 | HSCGF liposome as well as preparation and application thereof |
| CN103751228A (en) * | 2014-01-27 | 2014-04-30 | 贵州神奇药物研究院 | Medicine for treating Alzheimer disease and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109394563A (en) * | 2018-11-06 | 2019-03-01 | 福建省海西细胞生物工程有限公司 | A kind of preparation method of stem cell media liposome and the application in skin care item |
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| CN106236601B (en) | 2019-04-30 |
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