CN106198464A - A kind of laser-induced fluorescence (LIF) food detector - Google Patents
A kind of laser-induced fluorescence (LIF) food detector Download PDFInfo
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- CN106198464A CN106198464A CN201510231349.0A CN201510231349A CN106198464A CN 106198464 A CN106198464 A CN 106198464A CN 201510231349 A CN201510231349 A CN 201510231349A CN 106198464 A CN106198464 A CN 106198464A
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Abstract
The present invention relates to a kind of laser-induced fluorescence (LIF) food detector, constitute including laser instrument, single mode circular waveguide and multimode circular waveguide composite construction device, circular optical waveguide sensing module, photoelectric conversion module, DSP data processing and control module, usb data interface module and computer.Described laser instrument, single mode circular waveguide and multimode circular waveguide composite construction device, circular optical waveguide sensing module, photoelectric conversion module, DSP data processing and control module, usb data interface module and computer are sequentially connected with, and described circular optical waveguide sensing module includes circular optical waveguide sensing element and capillary sample pond.The invention have the benefit that described laser-induced fluorescence (LIF) food detector utilizes laser excitation to make the determinand being marked with fluorescence being connected on circular optical waveguide sensing element surface send fluorescence and collect by biological compatible reaction, it is then passed through Signal sampling and processing, the concentration of determinand in food can be detected.
Description
Technical field
The present invention relates to technical field of food detection, particularly relate to a kind of laser-induced fluorescence (LIF) food detector.
Background technology
Food additive, pesticide residues are especially exceeded standard and add the related data information growing interest of unauthorized substances in food by the safe condition of modern society people growing interest food.
Bio-detector based on circular optical waveguide utilizes laser to produce evanescent waves at sensor surface when transmitting in total reflection mode in circular waveguide, this evanescent waves, by exciting the test substance of the mark fluorescent being attached on sensor surface by biological compatible reaction, realizes the detection of test substance concentration by detection fluorescence signal size.The generally penetration depth of disappearance wave field only has tens nanometer to hundreds of nanometer, so circular optical waveguide bio-detector can only detect is incorporated into the fluorescence that the fluorescent material in the range of disappearance wave field sends, and fluorescent material free in solution produces impact to testing result hardly.Therefore such detector has that highly sensitive, biologic specificity is strong and the feature such as detection speed is fast, can dynamically detect bioprocesses simultaneously, thus is widely used in the field such as food inspection and environmental monitoring.
Such detector of prior art mainly utilization circular waveguide is as sensing unit, and optical signal collector unit uses a large amount of optical fractionation elements.Transmitting laser with the collection of fluorescence and detection is all to use conventional optical element, and such as reflecting mirror, battery of lens, chopper etc., therefore such detector exists the shortcomings such as optical path adjusting difficulty, system complex, energy loss are big.Laser-induced fluorescence (LIF) food detector of the present invention is that laser transmits and the collection of fluorescence all utilizes circular optical waveguide to realize, and has system structure simple, and light loss is few, detection sensitivity advantages of higher.
Summary of the invention
It is an object of the invention to provide a kind of laser-induced fluorescence (LIF) food detector, the composite construction using single mode circular waveguide and multimode circular waveguide composition realizes the transmission of laser and the collection of fluorescence, reduce optical fractionation device to greatest extent and realize the transmission of high efficiency light and high signal to noise ratio, finally realize the highly sensitive detection of food risk pollutant, to overcome deficiencies of the prior art.
It is an object of the invention to be achieved through the following technical solutions:
A kind of laser-induced fluorescence (LIF) food detector, including laser instrument, single mode circular waveguide and multimode circular waveguide composite construction device, circular optical waveguide sensing module, photoelectric conversion module, DSP data processing and control module, usb data interface module and computer, described laser instrument, single mode circular waveguide and multimode circular waveguide composite construction device, circular optical waveguide sensing module, photoelectric conversion module, DSP data processing and control module, usb data interface module and computer are sequentially connected with according to corresponding input/output port, the corresponding port of described DSP data processing and control module connects usb data interface module and photoelectric conversion module respectively；The front end of described photoelectric conversion module is equipped with fluorescent optical filter；Described circular optical waveguide sensing module includes circular optical waveguide sensing element and capillary sample pond.
Further, described single mode circular waveguide and multimode circular waveguide composite construction device are coupled to form by single mode circular waveguide and multimode circular waveguide bundle.
Further, a diameter of 4 microns of described single mode circular optical waveguide, numerical aperture is 0.22；A diameter of 200 microns of multimode circular waveguide bundle, numerical aperture is 0.22, is formed by 5 altogether.
Further, described circular waveguide sensing module uses dismountable adapter to be attached with single mode circular waveguide and multimode circular waveguide composite construction device.
Further, described circular waveguide sensing element surface is fixed with biological identification molecule.
The detection method of above-mentioned laser-induced fluorescence (LIF) food detector, comprises the following steps:
(1) information gathering of the concentration of determinand:
Utilize laser excitation to make the determinand being marked with fluorescence being connected on circular optical waveguide sensing element surface send fluorescence and collect by biological compatible reaction, then the information of collection is converted to the signal of telecommunication through photoelectric conversion module；
(2) amplification of signal, Filtering Processing: the signal of telecommunication of opto-electronic conversion is amplified through amplifying circuit, after the filtering of filter element, sends into DSP data processing module and further analyze and process；
(3) DSP data processing and control module is to the analyzing and processing of data and control: select different sample frequencys to carry out AD sampling, calculate and the analysis of DSP data processing module through fast Fourier transform, draw the signal characteristic value of testing concentration in certain period of time；It is sent to computer analysis through usb data interface module, and carries out corresponding data process and display.
The invention have the benefit that described laser-induced fluorescence (LIF) food detector volume is little to be convenient for carrying, scene it is directly placed at during use, laser excitation is utilized to make the determinand being marked with fluorescence being connected on circular optical waveguide sensing element surface send fluorescence and collect by biological compatible reaction, it is then passed through suitable Signal sampling and processing, the concentration of determinand in food can be detected.
Accompanying drawing explanation
Below according to accompanying drawing, the present invention is described in further detail.
In figure:
Fig. 1 is the structural principle schematic diagram of the laser-induced fluorescence (LIF) food detector described in the embodiment of the present invention；
Fig. 2 is the circular optical waveguide sensing module schematic diagram of the laser-induced fluorescence (LIF) food detector described in the embodiment of the present invention；
Fig. 3 is the sensing element schematic diagram of the laser-induced fluorescence (LIF) food detector described in the embodiment of the present invention；
Fig. 4 is single mode circular waveguide and the multimode circular waveguide composite construction device schematic diagram of the laser-induced fluorescence (LIF) food detector described in the embodiment of the present invention.
In figure:
1, laser instrument；2, single mode circular waveguide and multimode circular waveguide composite construction；3, photoelectric conversion module；4, fluorescent optical filter；5, adapter；6, circular optical waveguide sensing module；7, DSP data processing and control module；8, usb data interface module；9, computer；10, circular waveguide sensing element；11, capillary sample pond；1001, covering；1002, sensing element (fibre core)；201, single mode circular waveguide；202, multimode circular waveguide bundle.
Detailed description of the invention
As Figure 1-4, a kind of laser-induced fluorescence (LIF) food detector described in the embodiment of the present invention, including laser instrument 1, single mode circular waveguide and multimode circular waveguide composite construction device 2, circular optical waveguide sensing module 6, photoelectric conversion module 3, DSP data processing and control module 7, usb data interface module 8 and computer 9, described laser instrument 1, single mode circular waveguide and multimode circular waveguide composite construction device 2, circular optical waveguide sensing module 6, photoelectric conversion module 3, DSP data processing and control module 7, usb data interface module 8 and computer 9 are sequentially connected with according to corresponding input/output port, the corresponding port of described DSP data processing and control module 7 connects usb data interface module 8 and photoelectric conversion module 3 respectively；The front end of described photoelectric conversion module 3 is equipped with fluorescent optical filter 4；Described circular optical waveguide sensing module 6 includes circular optical waveguide sensing element 10 and capillary sample pond 11.
The laser that described laser instrument 1 sends single mode circular waveguide 201 in single mode circular waveguide and multimode circular waveguide composite construction device 2 enters the circular optical waveguide sensing element 10 in circular optical waveguide sensing module 6, and producing evanescent waves at sensing element (fibre core) 1002, this evanescent waves excites the fluorescence molecule being attached to the labelling of sensing element (fibre core) 1002 on the test object.Part fluorescence is coupled back into sensing element (fibre core) 1002, the multimode circular waveguide bundle 202 of single mode circular waveguide and multimode circular waveguide composite construction 2 is entered through adapter 5, fluorescent optical filter 4 filters the exciting light of reflection, and major part fluorescence is passed through, the fluorescence passed through is detected by photoelectric conversion module 3 and is converted into the signal of telecommunication, and then this signal transfers to computer 9 analyze in real time and show through usb data interface module 8 after DSP data processing and control module 7 processes.
Described single mode circular waveguide and multimode circular waveguide composite construction 2 are made up of a single mode circular waveguide 201 being in center and multimode circular waveguide bundle 202, wherein single mode circular waveguide 201 is that laser introduces circular waveguide sensing element 1002, and multimode circular waveguide bundle 202 is used for collecting fluorescence, be conducive to improving phosphor collection efficiency.
The covering 1001 of described circular optical waveguide sensing element 10 is removed part, and fixes biological identification molecule on sensing element (fibre core) 1002 surface.
Described single mode circular waveguide and multimode circular waveguide composite construction 2 are combined with circular optical waveguide sensing module 6 by adapter 5.
Described circular optical waveguide sensing module 6 is made up of circular optical waveguide sensing element 10 and capillary sample pond 11, sensing element (fibre core) 1002 needs fixing biological identification molecule before detection, it is then placed within capillary sample pond 11, then is connected with single mode circular waveguide and multimode circular waveguide composite construction 2.
During detection, circular optical waveguide sensing module 6 is put in determinand solution, under capillarity, solution enters capillary tube, after reaction certain time, open laser instrument 1, exciting light will enter sensing element (fibre core) 1002 in total reflection mode, the evanescent waves that laser produces during multihop propagation in sensing element (fibre core) 1002 excites the fluorescent material being attached to its surface, fluorescent material sends fluorescence, part is coupled back into sensing element (fibre core) 1002, after multimode circular optical waveguide bundle 202 transmits, penetrate from the other end, photoelectric conversion module 3 is entered after fluorescent optical filter 4 filters, and it is converted into the signal of telecommunication being directly proportional to fluorescence intensity, after DSP data processing and control module 7 processes, it is sent to computer 9 through usb data interface module 8 be analyzed processing.After computer 9 analyzing and processing to data be directly proportional to the concentration of circular optical waveguide element surface test substance, the concentration of test substance can be learnt by the power of analysis signal.
Fig. 1 is highly preferred embodiment of the present invention, and its concrete structure and parameter are as follows:
Excitation source be centre wavelength be the semiconductor laser 1 of 650nm, output is 5mW, in single mode circular waveguide and multimode circular waveguide composite construction: a diameter of 4 microns of single mode circular optical waveguide, numerical aperture is 0.22；A diameter of 200 microns of multimode circular waveguide, numerical aperture is 0.22, is formed by 5 altogether.The fluorescent optical filter 4 transmitance≤10-6 to exciting light, transmitance >=75% to fluorescence.Optical-electrical converter is photodiode.
During detection, being filled with the testing sample solution that marked Cy5.5 fluorescence molecule in capillary sample pond 8, in embodiment, circular waveguide element all reaches 10-8mol/L to the detectivity of Cy5.5 fluorescence molecule solution.Complete first order fluorescence signal detection required time less than 20min.
The present invention is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under the enlightenment of the present invention; though but in its shape or structure, make any change, every have same as the present application or akin technical scheme, within all falling within protection scope of the present invention.
Claims (6)
- null1. a laser-induced fluorescence (LIF) food detector，It is characterized in that: described laser-induced fluorescence (LIF) food detector includes laser instrument (1)、Single mode circular waveguide and multimode circular waveguide composite construction device (2)、Circular optical waveguide sensing module (6)、Photoelectric conversion module (3)、DSP data processing and control module (7)、Usb data interface module (8) and computer (9)，Described laser instrument (1)、Single mode circular waveguide and multimode circular waveguide composite construction device (2)、Circular optical waveguide sensing module (6)、Photoelectric conversion module (3)、DSP data processing and control module (7)、Usb data interface module (8) and computer (9) are sequentially connected with according to corresponding input/output port，The corresponding port of described DSP data processing and control module (7) connects usb data interface module (8) and photoelectric conversion module (3) respectively；The front end of described photoelectric conversion module (3) is equipped with fluorescent optical filter (4)；Described circular optical waveguide sensing module (6) includes circular optical waveguide sensing element (10) and capillary sample pond (11).
- Laser-induced fluorescence (LIF) food detector the most according to claim 1, it is characterised in that: described single mode circular waveguide and multimode circular waveguide composite construction device (2) are coupled to form by single mode circular waveguide (201) and multimode circular waveguide bundle (202).
- Laser-induced fluorescence (LIF) food detector the most according to claim 1, it is characterised in that: a diameter of 4 microns of described single mode circular optical waveguide (201), numerical aperture is 0.22；A diameter of 200 microns of multimode circular waveguide bundle (202), numerical aperture is 0.22, is formed by 5 altogether.
- Laser-induced fluorescence (LIF) food detector the most according to claim 1, it is characterised in that: described circular waveguide sensing module (6) uses dismountable adapter (5) to be attached with single mode circular waveguide and multimode circular waveguide composite construction device (2).
- Laser-induced fluorescence (LIF) food detector the most according to claim 1, it is characterised in that: described circular waveguide sensing element (1002) surface is fixed with biological identification molecule.
- 6. the detection method of the laser-induced fluorescence (LIF) food detector described in any one of claim 1-5, it is characterised in that comprise the following steps:(1) information gathering of the concentration of determinand: utilize laser excitation to make the determinand being marked with fluorescence being connected on circular optical waveguide sensing element surface send fluorescence and collect by biological compatible reaction, then the information of collection is converted to the signal of telecommunication through photoelectric conversion module；(2) amplification of signal, Filtering Processing: the signal of telecommunication of opto-electronic conversion is amplified through amplifying circuit, after the filtering of filter element, sends into DSP data processing module and further analyze and process；(3) DSP data processing and control module is to the analyzing and processing of data and control: select different sample frequencys to carry out AD sampling, calculate and the analysis of DSP data processing module through fast Fourier transform, draw the signal characteristic value of testing concentration in certain period of time；It is sent to computer analysis through usb data interface module, and carries out corresponding data process and display.
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| CN201540249U (en) * | 2009-06-25 | 2010-08-04 | 华东师范大学 | Automatic laser induced fluorescence spectroscopy measurement device |
| CN201852788U (en) * | 2010-11-11 | 2011-06-01 | 江阴弘扬传感探测技术有限公司 | Portable fluorescence detector |
| CN103245641A (en) * | 2012-02-10 | 2013-08-14 | 清华大学 | Multi-channel planar waveguide evanescent wave biosensor |
| CN204575538U (en) * | 2015-05-08 | 2015-08-19 | 罗强 | A kind of laser-induced fluorescence (LIF) food detector |
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2015
- 2015-05-08 CN CN201510231349.0A patent/CN106198464A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1873450A (en) * | 2006-06-30 | 2006-12-06 | 清华大学 | Biosensor of full fiber optic evanescent wave |
| CN101082584A (en) * | 2007-06-28 | 2007-12-05 | 大连海事大学 | Optical fiber biological sensor |
| CN101666747A (en) * | 2008-09-04 | 2010-03-10 | 北京金达清创环境科技有限公司 | Array fibre evanescent wave biosensor system |
| CN201540249U (en) * | 2009-06-25 | 2010-08-04 | 华东师范大学 | Automatic laser induced fluorescence spectroscopy measurement device |
| CN201852788U (en) * | 2010-11-11 | 2011-06-01 | 江阴弘扬传感探测技术有限公司 | Portable fluorescence detector |
| CN103245641A (en) * | 2012-02-10 | 2013-08-14 | 清华大学 | Multi-channel planar waveguide evanescent wave biosensor |
| CN204575538U (en) * | 2015-05-08 | 2015-08-19 | 罗强 | A kind of laser-induced fluorescence (LIF) food detector |
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Application publication date: 20161207 |