CN106174561A - Improve Rhizoma Curculiginis oral liquor and the oral liquid thereof of immunity - Google Patents

Abstract

The invention discloses a preparation method of curculigo curculigo oral liquid for improving immunity. The preparation steps include: a. extracting curculigo curculiosa as component 1; b. putting the remaining raw materials into ethanol and heating and refluxing to extract 2. Second, as component 2; c. Combine the above two extracts, component 1 and component 2, concentrate, add dextrin to reconcile, and prepare oral liquid after disinfection. In the step a, chop the collected curculigo, then slowly add 70% ethanol solution; wait until the air between the powder particles is discharged, and about 20L of ethanol flows out, close the discharge valve, and cover the drum , Dipping for 24 hours, and then open the material valve for percolation. The invention can better improve sub-healthy people who are prone to colds, sore eyes, poor sleep quality, spleen deficiency and fatigue, lack of food, dry mouth and thirst.

Description

Preparation method of Curculigo curculigo oral liquid for improving immunity and oral liquid thereof

technical field

The invention relates to a health product for improving immunity containing components derived from plants, in particular to a curculigo curculigo oral liquid for improving immunity and a preparation method thereof.

Background technique

There is no such term as "low immunity" in Chinese medicine, but there are similar concepts such as qi deficiency and physical weakness. Patients are prone to infection symptoms such as colds, pharyngitis, and pneumonia, because the body's righteousness is insufficient, and external evils are easy to invade repeatedly. Western medicine interprets it as low immunity, which means the same thing, so improving immunity is actually the goal pursued by both Chinese and Western medicine.

The so-called immunity, simply put, is the ability of the human body to avoid various pathogenic microorganisms. It should be said that in the long evolutionary process of human beings, human beings have been fighting against various pathogenic microorganisms in nature, including viruses, bacteria, fungi, etc. No matter how far science and technology advances, no matter how advanced medicines humans can invent, the body's own immunity will continue to play an important role.

Human immunity is the defense function of the human body against external pathogenic microorganisms, or the defense system. Under normal circumstances, it can ensure that the human body is not invaded by external pathogenic microorganisms. Generally speaking, human immunity refers to the ability of the human body's immune system to resist the invasion of foreign pathogenic microorganisms. Under the condition of normal human immunity, the human body itself can resist the invasion of quite a few germs and avoid causing damage to the human body. Immunity plays an important role in the mechanism of human life, and the human body's nutrition, fatigue, mood, environment, disease, medication, radiation, etc. lead to the decline of its own immunity. For millions of years, humans have lived in an environment that is both suitable for survival and fraught with danger. Human beings have survived and acquired extraordinary immunity. So immunity is a product of biological evolution.

A body with a low immune system is prone to infection or cancer; an immune system with a high immune system can also produce harmful results, such as triggering allergic reactions, autoimmune diseases, etc. For various reasons, the immune system cannot normally play a protective role. In this case, it is very easy to cause infections such as bacteria, viruses, and fungi. Therefore, the most direct manifestation of low immunity is that it is easy to get sick. Due to frequent illnesses, the consumption of the body has been aggravated, so there are generally symptoms such as physical weakness, malnutrition, listlessness, fatigue, loss of appetite, sleep disturbance, etc. Getting sick, taking injections and taking medicine have become commonplace. It takes a long time to recover from each illness, and often relapses. If things go on like this, it can lead to poor physical and mental development, and it is also easy to induce major diseases. The underlying reason is low immunity or unsound immunity. When the human body's immune function is dysfunctional, or the immune system is not perfect, the following problems will recur: --recurrent colds, recurrent tonsillitis, recurrent asthma, recurrent bronchitis, recurrent pneumonia, recurrent diarrhea... ...so don't underestimate it.

Immunity refers to the ability of the body to resist external invasion and maintain the stability of the internal environment. The air is full of all kinds of microorganisms: bacteria, viruses, mycoplasma, chlamydia, fungi, etc. In the case of low human immunity, they can all become pathogens of the morning shift. Although the human body will produce corresponding antibodies against different pathogens to resist re-infection, antibodies are specific and time-limited. Can not resist other virus infection. People with low immunity cannot resist the invasion of cold viruses at all, which is the real reason why susceptible people often catch colds.

Curculigo orchioides (scientific name: Curculigo orchioides Gaertn.), also known as ground palm (Sichuan, Guizhou), monophyll (Sichuan), mountain codonopsis (Fujian), curculigo orchioides (Yunnan), Hainan ginseng (Hainan), Mao claw, Brahmin Ginseng belongs to Curculigoaceae, flowering period is from April to September. Medicinal plants have the functions of tonifying kidney and yang, benefiting essence and blood, strengthening muscles and bones, promoting blood circulation and reducing swelling. Curculigo can warm kidney and strengthen Yang; dispel cold and dampness. Indicates impotence and essence cold; urinary incontinence; epigastric cold and pain; waist and knee pain; weakness of muscles and bones; spasms of lower limbs; menopausal syndrome.

Pharmacological effects of curculigo

effect on immune function

1. Effects on the phagocytosis of macrophages: mice were fed with 70% curculigo infusion 10, 20g/kg, once a day, for 7 consecutive days, and on the fifth day after administration, 0.4% liver 2 ml of glycogen, and 1 ml of 2% chicken red blood cell suspension was intraperitoneally injected 1 hour after the last administration, and the mice were sacrificed 2 hours later. The phagocytic function was measured according to the literature (Zhang Yunfen et al., Journal of Beijing Medical College, 1979). The results showed that Curculigo curculigo can significantly increase the phagocytosis percentage and phagocytosis index of mouse peritoneal macrophages compared with the control group.

2. Effects on the percentage of T lymphocytes in normal and immune-suppressed mice: The percentage of T lymphocytes in mice was determined by esterase method (Nanjing Railway Medical College Histoembryology Teaching and Research Office, Chinese Medical Journal, 1980). The results proved that Curculigo curculigo could not increase the percentage of T lymphocytes in normal mice, but it had a significant effect on the reduction of T lymphocytes in mice with immune function suppressed by cyclophosphamide.

3. The water extract of curculigo can promote the production of antibodies and prolong its efficacy. Curculigoside can promote the proliferation of macrophages and improve their phagocytosis, which can be considered to enhance immune function.

Effects on the central nervous system

1. Effect on sleep time of pentobarbital sodium: 30 minutes after intraperitoneal injection of curculigo alcohol infusion 10g/kg to mice, intraperitoneal injection of pentobarbital sodium 40mg/kg, recording sleep time of mice, and administration of results The average sleep time of the mice in the control group and the control group (normal saline) were 304.9±24.2 and 63.4±11.6 (minutes), respectively. It is proved that curculigo can significantly prolong sleep time (P<0.001).

2. The effect on convulsions induced by picrotoxin: 30 minutes after the intraperitoneal injection of Curculigo grass, 200 mg/kg of picrotoxin was injected intraperitoneally, and the latency time for the mice to appear clonic convulsions was recorded. The results showed that the latency time of convulsions in the curculiculatus group and the control group were 24.38±3.78 and 7.57±0.63 minutes respectively, and the difference between the two groups was very significant (P<0.001). It shows that curculigo can significantly delay the latent period of convulsions in mice induced by picrotoxin.

3. The stone alkali contained in Curculigo curculigo can significantly prolong the latent period of its conditioned reflex by intraperitoneal injection of 5 mg/kg to rats, so that the positive conditioned reflex partially disappears and then recovers. Injecting 2 mg/kg into mice has a significant effect. Sedative effect, a dose of 12mg/kg can prolong the hypnotic time of pentobarbital, and has significant analgesic and antipyretic effects.

Effects on hypothalamic-pituitary-gonadal axis function

1. Effects on endocrine function of pituitary gland and ovary in normal rats: 35 normal female rats, weighing 150-200g, were randomly divided into 8 groups: curculigo grass group, dodder group, Morinda officinalis group, Rehmannia glutinosa group, Cistanche deserticola group , Epimedium group, Lycium barbarum group and control group. The drug group (decoction) was administered by intragastric administration, 1ml/100g body weight, twice a day, for 5 days. The control group was intragastrically administered the same amount of normal saline. On the 6th day, the animals were treated, and under pentobarbital sodium anesthesia, 1 ml of blood was collected from the abdominal aorta, and the plasma was separated for determination of plasma luteinizing hormone (LH). The anterior lobe of the pituitary gland, uterus and ovary were removed and weighed, and the ovary was immediately put into the frozen Tris-Hcl buffer for determination of chorionic gonadotropin/luteinizing hormone receptor. The results showed that curculigo and other traditional Chinese medicines for promoting yang and tonifying kidney could significantly increase the weight of anterior pituitary gland, ovary and uterus in rats compared with the control group (P<0.01); but the level of luteinizing hormone in plasma did not change; The specific binding ability of gonadotropin/luteinizing hormone receptors in the medication group was also significantly higher than that in the control group (P<0.01).

2. Effects on the responsiveness of the pituitary gland of ovariectomized rats to luteinizing releasing hormone: the experimental design refers to the method of JohnsonJH and Davis CL (1981). Take 35 adult healthy female rats, weighing 150-200 g, and remove both ovaries under ether anesthesia. One week later, they were randomly divided into 8 groups, 7 groups were the corresponding medication groups, and 1 group was the control group. Oral administration twice a day, at 1ml/100g body weight, for 5 consecutive days; the control group took the same amount of normal saline. At 8 o'clock in the morning on the sixth day, anesthetize with pentobarbital sodium subcutaneous injection (50mg/kg), 30 minutes later, draw 0.4ml of blood from the abdominal cavity vein (heparin anticoagulant), and then inject D-alcohol-luteinizing hormone from the saphenous vein Release hormone (100ng/100g body weight). At 30 minutes and 90 minutes after the injection of luteinizing releasing hormone, 0.4 ml of blood was collected from the abdominal vein. After centrifugation for 3 times, the plasma of the blood sample was reserved for the determination of luteinizing hormone to determine the response of the pituitary gland to the secretion of luteinizing hormone after injection of luteinizing releasing hormone. The results showed that the levels of plasma luteinizing hormone in each group of animals were very low under pentobarbital sodium anesthesia. After injection of D-alpha-luteinizing releasing hormone, the plasma luteinizing hormone level in control animals increased to 22.25 ng/ml at 30 minutes and 18.50 ng/ml at 90 minutes. However, in the rats taking curculigo and other traditional Chinese medicines for strengthening yang and nourishing the kidney, the pituitary gland significantly increased the secretion of luteinizing hormone after injection of luteinizing releasing hormone, and the plasma luteinizing hormone level was 55.90 ng/ml at 90 minutes after injection, which was higher than that of the control group. group significantly increased (P <0.01).

androgen-like effect

Take 16 male rats with a body weight of 60-90g, remove the testes on both sides, divide them into two groups on the 7th day after the operation, and give 10g/kg of Curculigo grass 70% alcohol infusion and normal saline, once a day, respectively. 21 consecutive days. On the next day after the last administration, the animals were sacrificed, and the seminal vesicles were dissected out and weighed. Results The seminal vesicle weights of the curculigo and control groups were 0.116±0.009 and 0.0679±0.003mg/g body weight respectively, and there was a significant difference (P<0.01). It shows that curculigo has a male hormone-like effect.

Adaptation as-is

1. Anti-hypoxia effect: gavage 20 and 40 g/kg of curculigo 70% alcohol infusion to the mice, and after 1 hour, place the mice in a 250ml jar with a stopper, seal the bottle mouth, and record the time of each group. Rat survival time. Results The survival time of the mice in the 20g/kg and 40g/kg curculigo alcohol infusion groups and the control group were 28.8±1.14, 33.6±1.19 and 26.8±0.77 (minutes, X±SE), respectively. Curculigo 40g/kg has obvious anti-hypoxia effect (P<0.001).

2. Anti-high temperature effect: intraperitoneal injection of Curculigo 70% alcohol infusion 10g/kg to mice, and after 30 minutes, place it in an incubator at 45±1°C, and die with the total number of mice in the drug group and the control group Half was used as a boundary, and the death rate of mice in each group was recorded. 5 of the 20 mice in the curculigo curculigo group died (mortality rate 25%), while in the 20 mice of the control group, 14 died (the mortality rate was 70%), and the difference was significant between the two groups (P< 0.05). It shows that curculigo has anti-high temperature effect.

anti-inflammatory effect

The mice were intraperitoneally injected with Curculigo 70% alcohol infusion 10g/kg, cortisone 50mg/kg and normal saline, and croton oil was used to induce inflammation 30 minutes after the administration. Results The swelling degrees of the ears of the mice in the curculiculatus group, the cortisone group and the control group were 9.25±1.23mg, 4.1±1.35mg and 14±1.04mg, respectively. It shows that curculigo can significantly inhibit the experimental inflammation of mice (P<0.05).

Effect on Na(+), K(+)-ATPase activity of erythrocyte membrane

The mice were fed with 12% water decoction of curculigo and other tonic traditional Chinese medicines at 6 g/kg, once a day, for 10 consecutive days. The control group was given an equal volume of water. Determination of enzyme activity. The results showed that tonic medicines such as curculigo can increase the activity of Na(+) and K(+)-ATPase.

antibacterial effect

The 100% decoction has inhibitory effect on Shigella flexneri, Shigella flexneri and Shigella sonnii by using flat trenching method.

Antitumor effect

The acetone extract of curculigo has inhibitory effect on Ehrlich ascites carcinoma solid tumor. Lycorine can inhibit the anaerobic glycolysis of ascites cancer cells in mice, but it does not affect their gasification and respiration. Since cancer cells generally use anaerobic alcoholysis as the main source of energy, it can be considered that Curculigo curculigo can affect the glucose metabolism of cancer cells. There is a certain interference effect.

other role

Curculigo curculigo water extract can dilate coronary arteries and strengthen the heart. Intravenous injection of 6g/kg to rabbits with slow arrhythmia can significantly increase heart rate, significantly reduce the number of mutant cells, inhibit the activity of purine system converting enzyme by about 20%, inhibit cholecystokinin by about 80%, and make thyroid function When the level of cyclic-adenosine monophosphate in the patient's serum decreases, the patient's serum cyclic-adenosine monophosphate level will rise, and if the level of cyclic-guanosine monophosphate is elevated, it will fall back, and the ratio of cyclic-adenosine-phosphate/cyclic-guanosine-phosphate tends to be normal. Intraperitoneal injection of lycorine 6mg/kg to rats can increase uric acid excretion, significantly inhibit mouse kidney succinate dehydrogenase, mildly lower blood pressure, and resist experimental arthritis.

The smell of traditional Chinese medicine is bitter, and many people do not want to take it for a long time; How to maximize the efficacy of Curculigo curculigo without harming the human body, and obviously improve the taste so that people can accept it is a problem that we urgently need to solve.

Contents of the invention

The technical problem to be solved by the present invention is that the immunity of modern people is becoming more and more fragile, environmental pollution, irregular work and rest, fast pace of life, unhealthy eating, and lack of abstinence cause the incidence of various diseases to increase. constant increase. Especially the so-called office workers are high-risk groups with low immunity. Most people are in a sub-healthy state, overdrawing their unhealthy bodies. Symptoms of most people are insomnia and dreaminess, easy to catch a cold, sore eyes, spleen deficiency and weakness; lack of food, dry mouth; thirst; kidney deficiency, soreness of waist and knees; Leprosy. How to find safe and effective active substance health care products, which can greatly improve immunity and enhance physical fitness has always been the key to people's expectation to solve the problem. Curculigo curculigo can lower blood pressure, lower blood sugar, lower blood fat, prevent atherosclerosis, improve immunity and antibacterial effects. Curculigo, lukewarm. Return liver, kidney channel. Tonifying the liver and kidney, astringent essence is solidified. It is used for dizziness, tinnitus, waist and knee pain, impotence, nocturnal emission, enuresis, frequent urination, uterine bleeding, profuse sweating, prostration, internal heat and quenching thirst. It has obvious diuretic, antihypertensive, antibacterial and elevated blood white blood cell effects, and is beneficial to the treatment of liver and kidney deficiency. It has obvious curative effect on dizziness, tinnitus, backache and other diseases caused by kidney yin deficiency, such as spermatorrhea and frequent urination. Because of its astringent and hemostatic effect, it is often used for menorrhagia, metrorrhagia and leukorrhea caused by women's physical weakness. It has a good therapeutic effect on common diseases of andrology and gynecology, and is widely used in the production of tonic medicines. Coupled with some traditional Chinese medicines that have a light taste and do not affect the taste of dogwood, they are easy to accept without affecting the taste, and can better achieve the effect of improving immunity.

In order to solve the above-mentioned technical problems, the present invention provides a Curculigo curculigo oral liquid for improving immunity. The oral liquid may include the following raw materials in parts by weight: 10-50 parts of Curculigo curculigo, 10-20 parts of Digupi, 10 parts of Atractylodes macrocephala ～20 parts, Rehmannia glutinosa 10～20 parts, Rhodiola 10～20 parts, Epimedium 10～20 parts, Panax notoginseng 10～20 parts, Astragalus 10～20 parts, Gynostemma 10～20 parts, Schisandra 10～20 parts , 10-20 parts of acacia bark, 10-20 parts of raspberry, 10-20 parts of poria cocos, 10-20 parts of Radix Polygoni Multiflori, 10-20 parts of mulberry, 10-20 parts of wolfberry, 10-20 parts of eyebright, 10 yams ～20 parts, Achyranthes bidentata 10～20 parts, Radix Paeoniae Rubra 10～20 parts, Corydalis Corydalis 10～20 parts, honeysuckle 10～20 parts.

Said curculigo oral liquid for improving immunity, said oral liquid may also include the following raw materials in parts by weight: 10-40 parts of curculigo, 10-15 parts of Digupi, 10-15 parts of Atractylodes macrocephala, 10-15 parts of Rehmannia glutinosa 10-15 parts of Rhodiola, 10-15 parts of Epimedium, 10-15 parts of Panax notoginseng, 10-15 parts of Astragalus, 10-15 parts of Gynostemma, 10-15 parts of Schisandra, 10-15 parts of Albizia Bark, 10-15 parts of raspberry, 10-15 parts of Poria cocos, 10-20 parts of Radix Polygoni Multiflori, 10-20 parts of mulberry, 10-20 parts of wolfberry, 10-20 parts of eyebright, 10-20 parts of yam, 10-20 parts of Achyranthes bidentata 20 parts, 10-20 parts of Radix Paeoniae Rubra, 10-20 parts of Corydalis Corydalis, 10-20 parts of Honeysuckle.

Said curculigo oral liquid for improving immunity, said oral liquid may also include the following raw materials in parts by weight: 10-50 parts of curculigo, 10-20 parts of Digupi, 10-20 parts of Atractylodes macrocephala, 10-20 parts of Rehmannia glutinosa 10-20 parts of rhodiola, 10-20 parts of epimedium, 10-20 parts of Panax notoginseng, 10-20 parts of astragalus, 10-20 parts of Jiaogulan, 10-20 parts of Schisandra chinensis, 10-20 parts of Albizia juniper, 10-20 parts of raspberry, 10-20 parts of Poria cocos, 10-15 parts of Radix Polygoni Multiflori, 10-15 parts of mulberry, 10-15 parts of wolfberry, 10-15 parts of eyebright, 10-15 parts of yam, 10-15 parts of Achyranthes bidentata 15 parts, 10-15 parts of Radix Paeoniae Rubra, 10-15 parts of Corydalis Corydalis, 10-15 parts of Honeysuckle.

In order to solve the above-mentioned technical problems, the present invention also provides a preparation method of curculigo curculigo oral liquid for improving immunity, characterized in that, the preparation steps of said oral liquid include:

a. The extraction of curculigo, as component 1;

b. put the rest of the raw materials into ethanol and heat and reflux to extract twice, as component 2;

c. Combining the above two extract components 1 and 2, concentrating, adding dextrin to reconcile, disinfecting and sterilizing to prepare an oral liquid.

In the step a, the collected curculigo can be chopped, soaked and placed in a stainless steel bucket with a cover, add 70% ethanol solution, the mass is 0.8-1 times that of the coarse particles, stir evenly, keep it moist and airtight for 1 hour Above, fully expand; wrap the filter plate at the bottom of the percolation cylinder with a gauze bag and pave it flat, then mix and loosen the wet and expanded drug sample, then use a stainless steel spoon to fill the powder, and evenly put it into the percolation cylinder, pack 10 -12 cm thick, press evenly with a T-shaped rod, and then follow the above operation, load layer by layer, pressurize properly, the powder filling should not exceed 2/3 of the height of the percolation cylinder; the powder surface is covered with stainless steel holes Press the plate firmly, open the discharge valve under the percolation cylinder, put a container, and then slowly add 70% ethanol solution; after the air between the powder particles is discharged, and about 20L of ethanol flows out, close the discharge valve , cover the drum, soak for 24 hours, then open the material valve for percolation, control the percolation speed, generally 1000g medicinal materials flow out 2-3ml per minute, put the filtrate into the liquid storage tank, and pour the ethanol solution when emptying Put it into the liquid storage tank; combine the leachate and let it stand for later use as component 1.

In the step b, the remaining raw materials can be soaked in 10 times the amount of ethanol for 10-15 days, then heated and extracted twice for 1-2 hours each time, the supernatant is removed, the extracts are combined, and filtered through 100-110 mesh. After ultrafiltration through an ultrafiltration column with a cut-off molecular weight of 6000-10000, the ultrafiltrate is concentrated under reduced pressure to an extract with a relative density of 1.36 at 80°C, heated and concentrated to a paste, and left to stand for later use to form component 2.

In the step c, the extract extracted in the above steps can be mixed in a double-effect vacuum concentrator, and the filtrate is first concentrated to a concentrated solution with a relative density of 1.32 at 80°C, and then refrigerated at 0-5°C for 24 hours Add 0.3% diatomite filter aid to the refrigerated liquid, filter, put the filtrate into a double-effect vacuum concentrator, concentrate to contain 0.1g extract per 1ml, add dextrin to reconcile, and prepare oral liquid after sterilization agent.

In order to solve the above-mentioned technical problems, the present invention also provides a preparation method of Curculigo curculigo Oral Liquid for improving immunity, and its preparation steps can also be:

a. The preparation of curculigo, as component 1;

b. chopping and extracting the rest of the raw materials as component 2;

c. Mix component 1 and component 2, concentrate and add appropriate amount of honey to blend, sterilize with ultraviolet light and bottle.

In the step a, the collected curculigo can be put into 5-10 times the amount of ethanol, soaked for 1-2 hours, heated and extracted twice, each time for 1-2 hours, the supernatant is removed, and the extract is combined, 100 Filter through -120 mesh; then add 10 times the amount of water to heat and reflux the dregs of other raw materials extracted by ethanol to extract twice, each time for 1 to 2 hours, combine the two extracts and let stand; combine the above two Combine the extracts, recover ethanol under reduced pressure, and concentrate to a concentration of 0.6g crude drug/mL. After suction filtration, the relative density of the filtrate is about 1.08 at 20°C; reduce the pressure to 0.03-0.08MPa, and keep the temperature at 60- 80 ℃, concentrated to a relative density of 1.20, the temperature of the extract at 60 ℃ -70 ℃, component 1.

In the step b, the remaining raw materials and medicinal materials can be mixed, chopped and passed through a 100-120 mesh sieve, and the fine powder is used after sieving, and the coarse particles are put into an acid and alkali resistant dipping pot, and dipped together with ethanol above 70 degrees at room temperature 15-30 days, then heat and reflux extraction twice, each time for 1-2 hours, combine the two extracts and let stand, filter, separate and take the filtrate; filter with gauze, add 50-60% ethanol to the residue, 60°C Continue to extract at -70°C for 2 hours, stir once every 10 minutes, filter through gauze, combine the extracts, concentrate into a paste and become component 2.

The beneficial technical effect of the invention lies in that the invention has the advantages of non-toxicity and being able to be completely absorbed by organisms. Curculigo curculigo can lower blood pressure, lower blood sugar, lower blood fat, prevent atherosclerosis, improve immunity and antibacterial effects. Curculigo lukewarm. Return liver, kidney channel. Tonifying the liver and kidney, astringent essence is solidified. It is used for dizziness, tinnitus, waist and knee pain, impotence, nocturnal emission, enuresis, frequent urination, uterine bleeding, profuse sweating, prostration, internal heat and quenching thirst. It has obvious diuretic, antihypertensive, antibacterial and elevated blood white blood cell effects, and is beneficial to the treatment of liver and kidney deficiency. Dizziness, tinnitus, backache and other symptoms caused by kidney yin deficiency, nocturnal emission, frequent urination and other symptoms have obvious curative effects; plus some Chinese medicinal materials that promote blood circulation, invigorate the spleen, dampness and nourish yin, most of these medicinal materials have a light taste, It does not affect the taste of Cornus officinalis tea, and has no unpleasant smell of traditional Chinese medicine that is difficult to swallow. It is easy to accept and can be taken for a long time. It can better improve sub-healthy people who are prone to colds, sore eyes, poor sleep quality, spleen deficiency and fatigue; eating less Dry mouth, thirst, kidney deficiency, soreness of the waist and knees, impotence, nocturnal emission, tinnitus, dark eyes, premature graying of beard and hair, body weakness and thinness, wind, leprosy and ringworm, can better achieve the effect of improving immunity.

detailed description

Human immunity is the defense function of the human body against external pathogenic microorganisms, or the defense system. Under normal circumstances, it can ensure that the human body is not invaded by external pathogenic microorganisms. Generally speaking, human immunity refers to the ability of the human body's immune system to resist the invasion of foreign pathogenic microorganisms. Under the condition of normal human immunity, the human body itself can resist the invasion of quite a few germs and avoid causing damage to the human body. Immunity plays an important role in the mechanism of human life, and the human body's nutrition, fatigue, mood, environment, disease, medication, radiation, etc. lead to the decline of its own immunity. More and more studies have shown that traditional Chinese medicine is playing an increasingly important role in the regulation of human immunity.

Curculigo orchioides (scientific name: Curculigo orchioides Gaertn.), also known as ground palm (Sichuan, Guizhou), monophyll (Sichuan), mountain codonopsis (Fujian), curculigo orchioides (Yunnan), Hainan ginseng (Hainan), Mao claw, Brahmin Ginseng belongs to Curculigoaceae, flowering period is from April to September. Medicinal plants have the functions of tonifying kidney and yang, benefiting essence and blood, strengthening muscles and bones, promoting blood circulation and reducing swelling. Curculigo can warm kidney and strengthen Yang; dispel cold and dampness. Indicates impotence and essence cold; urinary incontinence; epigastric cold and pain; waist and knee pain; weakness of muscles and bones; spasms of lower limbs; menopausal syndrome.

Pharmacological effects of curculigo

effect on immune function

1. Effects on the phagocytosis of macrophages: mice were fed with 70% curculigo infusion 10, 20g/kg, once a day, for 7 consecutive days, and on the fifth day after administration, 0.4% liver 2 ml of glycogen, and 1 ml of 2% chicken red blood cell suspension was intraperitoneally injected 1 hour after the last administration, and the mice were sacrificed 2 hours later. The phagocytic function was measured according to the literature (Zhang Yunfen et al., Journal of Beijing Medical College, 1979). The results showed that Curculigo curculigo can significantly increase the phagocytosis percentage and phagocytosis index of mouse peritoneal macrophages compared with the control group.

2. Effects on the percentage of T lymphocytes in normal and immune-suppressed mice: The percentage of T lymphocytes in mice was determined by esterase method (Nanjing Railway Medical College Histoembryology Teaching and Research Office, Chinese Medical Journal, 1980). The results proved that Curculigo curculigo could not increase the percentage of T lymphocytes in normal mice, but it had a significant effect on the reduction of T lymphocytes in mice with immune function suppressed by cyclophosphamide.

3. The water extract of curculigo can promote the production of antibodies and prolong its efficacy. Curculigoside can promote the proliferation of macrophages and improve their phagocytosis, which can be considered to enhance immune function.

Effects on the central nervous system

1. Effect on sleep time of pentobarbital sodium: 30 minutes after intraperitoneal injection of curculigo alcohol infusion 10g/kg to mice, intraperitoneal injection of pentobarbital sodium 40mg/kg, recording sleep time of mice, and administration of results The average sleep time of the mice in the control group and the control group (normal saline) were 304.9±24.2 and 63.4±11.6 (minutes), respectively. It is proved that curculigo can significantly prolong sleep time (P<0.001).

2. The effect on convulsions induced by picrotoxin: 30 minutes after the intraperitoneal injection of Curculigo grass, 200 mg/kg of picrotoxin was injected intraperitoneally, and the latency time for the mice to appear clonic convulsions was recorded. The results showed that the latency time of convulsions in the curculiculatus group and the control group were 24.38±3.78 and 7.57±0.63 minutes respectively, and the difference between the two groups was very significant (P<0.001). It shows that curculigo can significantly delay the latent period of convulsions in mice induced by picrotoxin.

3. The stone alkali contained in Curculigo curculigo can significantly prolong the latent period of its conditioned reflex by intraperitoneal injection of 5 mg/kg to rats, so that the positive conditioned reflex partially disappears and then recovers. Injecting 2 mg/kg into mice has a significant effect. Sedative effect, a dose of 12mg/kg can prolong the hypnotic time of pentobarbital, and has significant analgesic and antipyretic effects.

Effects on hypothalamic-pituitary-gonadal axis function

1. Effects on endocrine function of pituitary gland and ovary in normal rats: 35 normal female rats, weighing 150-200g, were randomly divided into 8 groups: curculigo grass group, dodder group, Morinda officinalis group, Rehmannia glutinosa group, Cistanche deserticola group , Epimedium group, Lycium barbarum group and control group. The drug group (decoction) was administered by intragastric administration, 1ml/100g body weight, twice a day, for 5 days. The control group was intragastrically administered the same amount of normal saline. On the 6th day, the animals were treated, and under pentobarbital sodium anesthesia, 1 ml of blood was collected from the abdominal aorta, and the plasma was separated for determination of plasma luteinizing hormone (LH). The anterior lobe of the pituitary gland, uterus and ovary were removed and weighed, and the ovary was immediately put into the frozen Tris-Hcl buffer for determination of chorionic gonadotropin/luteinizing hormone receptor. The results showed that curculigo and other traditional Chinese medicines for promoting yang and tonifying kidney could significantly increase the weight of anterior pituitary gland, ovary and uterus in rats compared with the control group (P<0.01); but the level of luteinizing hormone in plasma did not change; The specific binding ability of gonadotropin/luteinizing hormone receptors in the medication group was also significantly higher than that in the control group (P<0.01).

2. Effects on the responsiveness of the pituitary gland of ovariectomized rats to luteinizing releasing hormone: the experimental design refers to the method of JohnsonJH and Davis CL (1981). Take 35 adult healthy female rats, weighing 150-200 g, and remove both ovaries under ether anesthesia. One week later, they were randomly divided into 8 groups, 7 groups were the corresponding medication groups, and 1 group was the control group. Oral administration twice a day, at 1ml/100g body weight, for 5 consecutive days; the control group took the same amount of normal saline. At 8 o'clock in the morning on the sixth day, anesthetize with pentobarbital sodium subcutaneous injection (50mg/kg), 30 minutes later, draw 0.4ml of blood from the abdominal cavity vein (heparin anticoagulant), and then inject D-alcohol-luteinizing hormone from the saphenous vein Release hormone (100ng/100g body weight). At 30 minutes and 90 minutes after the injection of luteinizing releasing hormone, 0.4 ml of blood was collected from the abdominal vein. After centrifugation for 3 times, the plasma of the blood sample was reserved for the determination of luteinizing hormone to determine the response of the pituitary gland to the secretion of luteinizing hormone after injection of luteinizing releasing hormone. The results showed that the levels of plasma luteinizing hormone in each group of animals were very low under pentobarbital sodium anesthesia. After injection of D-C-LH, the plasma LH level of the animals in the control group increased to 22.25 ng/ml at 30 minutes and 18.50 ng/ml at 90 minutes. However, in the rats taking curculigo and other traditional Chinese medicines for strengthening yang and nourishing the kidney, the pituitary gland significantly increased the secretion of luteinizing hormone after injection of luteinizing releasing hormone, and the plasma luteinizing hormone level was 55.90 ng/ml at 90 minutes after injection, which was higher than that of the control group. group significantly increased (P <0.01).

androgen-like effect

Take 16 male rats with a body weight of 60-90g, remove the testes on both sides, divide them into two groups on the 7th day after the operation, and give 10g/kg of Curculigo grass 70% alcohol infusion and normal saline, once a day, respectively. 21 consecutive days. On the next day after the last administration, the animals were sacrificed, and the seminal vesicles were dissected out and weighed. Results The seminal vesicle weights of the curculigo and control groups were 0.116±0.009 and 0.0679±0.003mg/g body weight respectively, and there was a significant difference (P<0.01). It shows that curculigo has a male hormone-like effect.

Adaptation as-is

1. Anti-hypoxia effect: gavage 20 and 40 g/kg of curculigo 70% alcohol infusion to the mice, and after 1 hour, place the mice in a 250ml jar with a stopper, seal the bottle mouth, and record the time of each group. Rat survival time. Results The survival time of the mice in the 20g/kg and 40g/kg curculigo alcohol infusion groups and the control group were 28.8±1.14, 33.6±1.19 and 26.8±0.77 (minutes, X±SE), respectively. Curculigo 40g/kg has obvious anti-hypoxia effect (P<0.001).

2. Anti-high temperature effect: intraperitoneal injection of Curculigo 70% alcohol infusion 10g/kg to mice, and after 30 minutes, place it in an incubator at 45±1°C, and die with the total number of mice in the drug group and the control group Half was used as a boundary, and the death rate of mice in each group was recorded. 5 of the 20 mice in the curculigo curculigo group died (mortality rate 25%), while in the 20 mice of the control group, 14 died (the mortality rate was 70%), and the difference was significant between the two groups (P< 0.05). It shows that curculigo has anti-high temperature effect.

anti-inflammatory effect

The mice were intraperitoneally injected with Curculigo 70% alcohol infusion 10g/kg, cortisone 50mg/kg and normal saline, and croton oil was used to induce inflammation 30 minutes after the administration. Results The swelling degrees of the ears of the mice in the curculiculatus group, the cortisone group and the control group were 9.25±1.23mg, 4.1±1.35mg and 14±1.04mg, respectively. It shows that curculigo can significantly inhibit the experimental inflammation of mice (P<0.05).

Effect on Na(+), K(+)-ATPase activity of erythrocyte membrane

The mice were fed with 12% water decoction of curculigo and other tonic traditional Chinese medicines at 6 g/kg, once a day, for 10 consecutive days. The control group was given an equal volume of water. Determination of enzyme activity. The results showed that tonic medicines such as curculigo can increase the activity of Na(+) and K(+)-ATPase.

antibacterial effect

The 100% decoction has inhibitory effect on Shigella flexneri, Shigella flexneri and Shigella sonnii by using flat trenching method.

Antitumor effect

The acetone extract of curculigo has inhibitory effect on Ehrlich ascites carcinoma solid tumor. Lycorine can inhibit the anaerobic glycolysis of ascites cancer cells in mice, but it does not affect their gasification and respiration. Since cancer cells generally use anaerobic alcoholysis as the main source of energy, it can be considered that Curculigo curculigo can affect the glucose metabolism of cancer cells. There is a certain interference effect.

other role

Curculigo curculigo water extract can dilate coronary arteries and strengthen the heart. Intravenous injection of 6g/kg to rabbits with slow arrhythmia can significantly increase heart rate, significantly reduce the number of mutant cells, inhibit the activity of purine system converting enzyme by about 20%, inhibit cholecystokinin by about 80%, and make thyroid function When the level of cyclic-adenosine monophosphate in the patient's serum decreases, the patient's serum cyclic-adenosine monophosphate level will rise, and if the level of cyclic-guanosine monophosphate is elevated, it will fall back, and the ratio of cyclic-adenosine-phosphate/cyclic-guanosine-phosphate tends to be normal. Intraperitoneal injection of lycorine 6mg/kg to rats can increase uric acid excretion, significantly inhibit mouse kidney succinate dehydrogenase, mildly lower blood pressure, and resist experimental arthritis

Codonopsis Codonopsis is a traditional tonic medicine commonly used in China. In ancient times, Codonopsis Codonopsis produced in Shangdang area of Shanxi was the top grade. It has the effects of nourishing the middle and Qi, strengthening the spleen and lungs. Modern research shows that Codonopsis pilosula contains various sugars, phenols, sterols, volatile oils, baicalein glucoside, saponins and trace alkaloids, which have the functions of enhancing immunity, dilating blood vessels, lowering blood pressure, improving microcirculation, and enhancing hematopoietic function. In addition, it can improve the leukopenia caused by chemotherapy and radiotherapy. Codonopsis pilosula has the effects of invigorating the middle and replenishing qi, invigorating the spleen and benefiting the lungs. For weakness of the spleen and lung, shortness of breath and palpitations, lack of food and loose stools, asthma and cough due to deficiency, internal heat and quenching thirst, etc. "Materia Medica Congxin" records: "Replenishing the middle and replenishing qi, harmonizing the spleen and stomach, and relieving polydipsia. The middle qi is weak, and it is very safe to use it for tonifying."

Poria cocos is sweet, light, and flat in nature. When used as medicine, it has the functions of diuresis and dampness, spleen and stomach, and calming the mind. Modern medical research: Poria cocos can enhance the immune function of the body, and Poria cocos polysaccharide has obvious anti-tumor and liver-protecting effects. Poria cocos is sweet and flat in nature and flavor, and enters the heart, lung and spleen meridians. It has the effects of eliminating dampness and diuresis, invigorating the spleen and stomach, calming the mind and calming the nerves. It can cure dysuria, edema and fullness, phlegm retention and cough, vomiting, nausea, diarrhea, spermatorrhea, stranguria, palpitations, and amnesia. The diuresis of Poria cocos is achieved by invigorating the functions of the spleen and lungs, which is different from other traditional Chinese medicines that directly diuresis. For spleen deficiency diarrhea, taking Poria cocos can not only strengthen the spleen, but also ooze dampness. For diarrhea and leukorrhea caused by spleen deficiency, Poria cocos has the effect of taking care of both specimens and is often compatible with Codonopsis pilosula, Atractylodes macrocephala, and Chinese yam. It can be used as an adjuvant medicine for invigorating the lung and spleen and treating qi deficiency.

Schisandra is warm; sour and sweet; returns to the lung, heart, and kidney meridians; astringent and astringent, nourishing qi and promoting body fluid, nourishing the kidney and calming the heart. For chronic cough and shortness of breath, nocturnal spermatorrhea, enuresis, frequent urination, chronic diarrhea, spontaneous sweating, night sweats, thirsty body fluid wounds, shortness of breath and arteries, internal heat and quenching thirst, palpitations and insomnia. "Compendium of Materia Medica" contains: Schisandra governs Qi, the lungs govern all Qi, acid can be absorbed, and it is entering the lungs to nourish the lungs, so it also replenishes Qi. It mainly focuses on those who cough against the upper qi, and if the qi is deficient, the upper qi will be obstructed and not return to the original source. If the acid is absorbed, the qi will be absorbed and returned to the original source, and the cough and upper qi will be eliminated by itself. Lose weight due to overwork, replenish deficiencies, strengthen yin, and benefit men's essence. "Bie Lu" nourishes the five viscera, removes heat, and grows the middle muscles of the yin. When the dross is turned into fine particles, the essence will be self-generated, and when the essence is born, the yin will grow, so it is the same as the above-mentioned diseases.

Rehmannia glutinosa, also known as Rehmannia glutinosa or Fudi, belongs to the plant of Scrophulariaceae. It is a good Chinese herbal medicine. It has the effect of nourishing blood and nourishing yin. It is one of the main ingredients of Liuwei Dihuang Pills, an over-the-counter drug for deficiency syndromes, which is used for hot flashes, night sweats, nocturnal emission, and thirst due to deficiency of kidney yin. This quality nourishes the kidneys, is good at nourishing kidney yin, replenishes essence and marrow, and is an essential medicine for invigorating kidney yin. The ancients called it "replenishing the five internal organs and true yin" and "replenishing true water". It is often used with yam and dogwood to treat liver and kidney yin deficiency, soreness of the waist and knees, nocturnal emission, night sweats, tinnitus, deafness and thirst, etc. It can nourish the liver and kidney, and benefit the essence, such as Liuwei Dihuang Wan.

Astragalus has the effects of invigorating qi and solidifying the surface, diuresis and swelling, detoxification and pus drainage, and muscle regeneration. The medicinal use of Astragalus has a history of more than 2,000 years. Modern research shows that Astragalus contains saponins, sucrose, polysaccharides, various amino acids, folic acid, selenium, zinc, copper and other trace elements. It can enhance the immune function of the body, protect the liver, diuretic, anti-aging, anti-stress, lower blood pressure and have a wide range of antibacterial effects. However, there are evidences such as exuberance of excess pathogens, stagnation of qi and dampness, stagnation of food accumulation, early onset of carbuncle or ulceration of heat and toxin. Radix Astragali has the effects of invigorating qi and strengthening the exterior, diuretic and detoxification, evacuating pus, astringing sores and promoting granulation. For qi deficiency and fatigue, lack of appetite, loose stools, subsidence of central qi, chronic diarrhea and prolapse of the anus, blood in the stool and metrorrhagia, spontaneous perspiration due to superficial deficiency, carbuncle and gangrene that are difficult to ulcerate, chronic ulceration without restraint, blood deficiency and chlorosis, internal heat and quenching thirst. "The Classic" records: "mainly carbuncle and gangrene, long-term sores, draining pus and relieving pain. Tonifies deficiency, and treats all kinds of diseases in children." "Nihuazi Materia Medica" records: "Helps Qi and strengthens muscles and bones, grows flesh and nourishes blood."

Radix Polygoni Multiflori is made by boiling raw Radix Polygoni Multiflori with black beans and then drying it in the sun. It is mainly used to treat symptoms such as weakness of the waist and knees, dizziness, palpitations, insomnia, and premature graying of hair caused by deficiency of liver and kidney, and loss of essence and blood. The black hair function of Polygonum multiflorum is exerted through the functions of nourishing the kidney, benefiting the essence, nourishing the blood and calming the mind. Shouwu has the functions of nourishing essence and blood, nourishing the liver and calming the nerves, strengthening the bones and muscles, and strengthening the kidneys. It is used for dizziness and dizziness due to blood deficiency, palpitation, insomnia, forgetfulness, chlorosis and fatigue, dizziness and tinnitus due to liver and kidney essence and blood deficiency, soreness of the waist and knees, Nocturnal emission collapse belt, premature graying of beard and hair embolism.

Digupi is cold in nature and sweet in taste. Functions and indications: cooling blood and removing steam, clearing lung and reducing fire. For yin deficiency hot flashes, bone steaming night sweats, lung heat cough, hemoptysis, epistaxis. "Bie Lu": "mainly rheumatism, lower chest and hypochondrium, heat headache, tonifying internal injuries, breathing, strengthening tendons, strengthening yin, benefiting large and small intestines, cold and heat." Cold and heat, all kinds of blood syndrome, epistaxis, coughing blood, cough, wheezing, diarrhea, stroke, dizziness, convulsions, low back pain, numbness, beriberi, edema, restlessness, palpitations, forgetfulness, difficulty urinating, red and white turbidity. "

Atractylodes macrocephala can significantly enhance the contraction amplitude and contraction frequency of small intestinal smooth muscle, prolong the contraction time of small intestinal smooth muscle under hypoxic conditions, adjust gastrointestinal motility function, and have the functions of anti-ulcer, liver protection and enhancing immune function of the body. Atractylodes macrocephala bitter in the mouth, sweet, warm. Return spleen, stomach warp. Cure mainly invigorating the spleen and replenishing qi, drying dampness and diuresis, antiperspirant, antiabortive. For insufficiency of the spleen, lack of food, abdominal distension and diarrhea, dizziness and palpitation due to phlegm and retention, edema, spontaneous sweating, restless fetal movement. Atractylodes macrocephala has cytotoxic effect on tumor cells, can reduce the proliferation rate of tumor cells, reduce the invasiveness of tumor tissues, and improve the body's anti-tumor response ability. "On the Nature of Medicine" contains it: Jun, sweet in the mouth, pungent, nontoxic. Can control strong wind numbness, dysentery due to dysentery for many years, confidant's distending pain, breaks away food, whets the appetite, removes phlegm and salivation, removes cold and heat, and stops diarrhea. The main face is radiant, calms the face, and removes blackness. Control water swelling and fullness, vomiting and nausea, cold pain in the abdomen, vomiting and diarrhea, and cold dysentery due to stomach qi deficiency.

Albizia Julibrissin is sweet and flat in nature and flavor. It has the functions of relieving depression, harmonizing blood, calming the heart, and eliminating carbuncle. It has the effect of treating restlessness, depression, insomnia, lung abscess, carbuncle, scrofula, and tendon fracture. "Shen Nong's Materia Medica": "Albizia julibrissin is sweet and flat. It is mainly for the five internal organs, benefiting the heart and mind, making people happy and worry-free... growing valleys." Albizia juniper is the bark of the leguminous plant Albizia julienne tree. In summer and autumn, the bark is peeled and dried for medicinal purposes. Nature and flavor are sweet, flat. It has the functions of relieving depression, harmonizing blood, calming the heart, and eliminating carbuncle. It has the effect of treating restlessness, depression, insomnia, lung abscess, carbuncle, scrofula, and tendon fracture.

Raspberry is warm in nature; sweet and sour; returns to kidney and bladder channels. Indications: Tonifying the kidney, strengthening essence, reducing urination. For kidney deficiency enuresis, frequent urination, impotence and premature ejaculation, nocturnal emission and spermatorrhea. "Compendium of Materia Medica": "Raspberry, its main benefit is Qi, and it is also said to benefit essence. Kidney stores essence, kidney receives Qi, and if essence is sufficient, the body will be light and the hair will not be white. Su Gong advocates tonic and continuation Absolutely, strengthens yin and builds yang, brightens the skin, harmonizes viscera. Zhen Quan masters men with kidney deficiency and impotence, and women eat and have children. Great Ming masters the five internal organs, improves color, nourishes essence, grows hair, and strengthens will All take its beneficial kidney to add essence, and the artificial ear of sweet and sour convergence." "On the Nature of Medicine" contains it: "The main man's kidney essence is exhausted, and the woman eats to have children. Main impotence."

Epimedium, epimedium is a commonly used traditional Chinese medicine in China, with high medicinal value, and the whole plant is used for medicinal purposes. Indications for impotence and premature ejaculation, backache and leg pain, numbness of limbs, hemiplegia, neurasthenia, forgetfulness, tinnitus, dizziness and other diseases.

Radix Notoginseng is sweet, slightly bitter, warm, and returns to the Liver and Stomach Meridians. Efficacy dissipating blood stasis to stop bleeding, reducing swelling and relieving pain. Cure mainly for hemoptysis, hematemesis, epistaxis, hematochezia, metrorrhagia, traumatic hemorrhage, chest and abdomen stabbing pain, tumbling and swelling pain. "Compendium of Materia Medica": Stop bleeding, disperse blood and relieve pain, for wounds caused by golden blade arrows, sores caused by falling sticks, and bleeding that does not stop, chew it and apply it, or mix it at the end, and the blood will stop immediately. It also mainly treats hematemesis and epistaxis, bloody dysentery, metrorrhagia, dysmenorrhea, postpartum hemorrhage, pain in blood circulation, red eyes and carbuncle, tiger bites and snake bites. "Materia Medica Seeking Truth": specially enters the liver and stomach. And into the heart and large intestine. Also known as mountain lacquer. Shi Zhenri. Or Yuneng alloy sores. Such as paint stickies also.

Lycium barbarum: sweet, flat. Lycium barbarum leaves: bitter, sweet; cool in nature. Function: Lycium barbarum: nourishes the liver, nourishes the kidney, moistens the lungs. Lycium barbarum leaves: tonifies deficiency and essence, clears away heat and improves eyesight. Lycium barbarum polysaccharide is a water-soluble polysaccharide, which is the most important active ingredient in Lycium barbarum, with a relative molecular mass of 68-200, and has become a research hotspot at home and abroad. Among them, the immune regulation and anti-tumor effects of Lycium barbarum polysaccharides are the most studied. Many studies have shown that Lycium barbarum polysaccharide has the functions of promoting immunity, anti-aging, anti-tumor, scavenging free radicals, anti-fatigue, anti-radiation, protecting liver, protecting and improving reproductive functions, etc.

Gynostemma, also known as: Paradise Grass, Gospel Grass, Super Ginseng, Gong Luo Pan Bottom, Rooting Everywhere, Aescin, Five-leaf Ginseng and Aescin, etc., Latin name: Gynostemmapentaphyllum (Thunb.) Makino Cucurbitaceae, Gynostemma genus Quality climbing plants; stems are thin and weak, with branches, longitudinal ribs and grooves, glabrous or sparsely pubescent, and the medicinal content of Gynostemma pentaphylla growing in the south is relatively high. Folks call it the magical "herb of longevity and longevity". The main active ingredients are gypenoside, gypenoside (polysaccharide), water-soluble amino acid, flavonoids, multivitamins, trace elements, minerals, etc. Efficacy: lowering blood fat, regulating blood pressure, preventing thrombosis, preventing and treating cardiovascular diseases, regulating blood sugar, promoting sleep, delaying aging, preventing and anti-cancer, improving immunity, and regulating human physiological functions. Gynostemma can protect the adrenal gland, thymus gland and endocrine organs from atrophy with age, maintain the function of the endocrine system, and have the effect of lowering blood sugar and improving glucose metabolism.

Rhodiola rosea (scientific name: Rhodiola rosea L.), alias: Rhodiola rosea, Saul Marble (Tibetan name), etc.; it is a perennial herb, 10-20 cm high. The root is thick, conical, fleshy, brownish yellow, with many fibrous roots at the root neck, the rhizome is short, thick, cylindrical, covered by many scale-like leaves arranged in tiles. It grows in the alpine and pollution-free zone at an altitude of 1800-2500 meters, and its growth environment is harsh, so it has strong vitality and special adaptability. It can be used as medicine, can invigorate qi and clear the lungs, improve intelligence and nourish the heart, and is a traditional Chinese medicine with a wide range of effects. It also has a great cosmetic effect and can be used as a skin care product or edible. Nature and flavor: cold, sweet, astringent. Meridian return: lung meridian. Functions and Indications: It has the effects of invigorating qi and clearing the lungs, improving intelligence and nourishing the heart, reducing astringency to stop bleeding, dispelling blood stasis and reducing swelling. It is mainly used to treat qi deficiency and frailty, chills after illness, shortness of breath and fatigue, cough due to lung heat, hemoptysis, leucorrhea diarrhea, bruises, etc. The first medical classic in ancient China, "Shen Nong's Materia Medica", listed Rhodiola as a top-grade medicine. Taking Rhodiola can lighten the body and nourish Qi, prevent aging and prolong life. It can invigorate the kidney, regulate qi and nourish blood, and treat general fatigue and chest tightness. It also has the effects of promoting blood circulation to stop bleeding, clearing lung and relieving cough, antipyretic, and stopping leukorrhea.

Mulberry nourishes yin and nourishes blood; promotes body fluid; moistens intestines. Enriches blood and nourishes yin, promotes fluid production and moisturizes dryness. For vertigo and tinnitus, palpitation and insomnia, premature graying of beard and hair, thirsty wounds, internal heat quenching one's thirst, blood deficiency and constipation. Invigorate the liver, nourish the kidney, quench the wind, and nourish the liquid. Treat liver and kidney yin deficiency, diabetes, constipation, dark eyes, tinnitus, scrofula, and joint discomfort. It can be eaten or used as medicine. Chinese medicine believes that mulberries are sweet and sour in taste and slightly cold in nature. They enter the heart, liver, and kidney meridians. It has the effects of nourishing blood and nourishing yin, promoting body fluid to quench thirst, moistening intestines and dryness, etc. It is mainly used to treat dizziness, tinnitus and palpitations, irritability and insomnia, soreness of waist and knees, premature graying of beard and hair, thirst and dry mouth, dry stool and other symptoms caused by deficiency of yin and blood.

Eyebright, scientific name: Euphrasia pectinata Ten, is an annual herb, and the whole plant has medicinal value. Bitter in the mouth; slightly cold in nature, returns bladder meridian, function cures mainly heat-clearing and toxic substances removing; Diuresis. Mainly fever and thirst; headache; lung heat cough; sore throat; hot stranguria; dysuria; aphtha; carbuncle.

Yam, also known as yam, native potato, yam, Huai yam, Huaishan, and white yam, is a herbal medicine recorded in "Chinese Materia Medica", and its medicinal source is the dried rhizome of yam, a plant of the family Dioscoreae. Yam has the functions of nourishing and strengthening, aiding digestion, suppressing sweating due to deficiency, and stopping diarrhea. Yam is most suitable to be taken with Ganoderma lucidum, which has the effect of preventing and treating diabetes. Yam has great development prospects in the food industry and processing industry.

Radix Paeoniae Rubra, the name of traditional Chinese medicine. It is the dry root of Radix Paeoniae Rubra or Radix Radix Radix Paeoniae Rubra of Ranunculaceae. Excavated in spring and autumn, removed rhizomes, fibrous roots and sediment, and dried in the sun. Bitter, slightly cold. Return liver channel. It has the effects of clearing heat and cooling blood, promoting blood circulation and removing blood stasis. "Shennong Materia Medica": "Peony, bitterness and flatness. Lord evil qi and abdominal pain, removing blood paralysis, breaking the cold and heat hernia, relieving pain ... Shengchuan Valley." "Famous Doctor" And hills, harvest roots and dry out in February and August."

Achyranthes bidentata root is used as medicine, used raw, promoting blood circulation and promoting menstruation; treating postpartum abdominal pain, irregular menstruation, amenorrhea, epistaxis, toothache due to deficiency fire, beriberi edema; cooked use, nourishing liver and kidney, strengthening waist and knees; treating soreness of waist and knees, liver and kidney deficiency Deficiency, bruises and bruises. Veterinarians use it to treat bovine soft feet, falls and broken bones. Medicinal part: the dry root of the plant. Nature and flavor: bitter, sweet, sour, flat. Return through: return liver, kidney channel. Efficacy: expel blood stasis to stimulate menstrual flow, nourish liver and kidney, strengthen muscles and bones, diuresis and relieve stranguria, induce blood to descend. Indications: For amenorrhea, dysmenorrhea, soreness of the waist and knees, weakness of muscles and bones, stranguria, edema, headache, dizziness, toothache, sun sore, vomiting blood, epistaxis.

Corydalis pungent; bitter; warm; nontoxic, meridian distribution: liver; stomach; heart; lung; spleen meridian. Indications: promoting blood circulation; dispelling blood stasis; regulating qi; relieving pain. Main confidant waist and knee pain; irregular menstruation; lump in the abdomen; metrorrhagia; postpartum haemorrhage; lochia endlessly; bruises. "Lei Gong Pao Shao Lun": cure heartache. "Nihuazi Materia Medica": eliminates wind, controls qi, warms the waist and knees, breaks symptoms, beats damage and blood stasis, abortion, and sudden lumbago. "Kaibao Materia Medica": Mainly breaks blood, and postpartum diseases are caused by blood. Irregular menstruation in women, lumps in the abdomen, metrorrhagia, postpartum blood circulation, rush of blood, bleeding due to damage, or rubbed with alcohol or boiled.

Honeysuckle, also known as honeysuckle (scientific name: Lonicera japonica). The name "Honeysuckle" comes from "Compendium of Materia Medica". Because the honeysuckle flower is white at first, and then turns yellow, it is named honeysuckle. The medicinal material honeysuckle is the dried flower buds or flowers of the same genus Lonicera Lonicera and the same genus. Honeysuckle has been known as a good medicine for clearing away heat and detoxification since ancient times. It is sweet and cold in nature and fragrant, sweet and cold to clear away heat without hurting the stomach, and the fragrance is thorough and can dispel evil. Honeysuckle can not only disperse wind-heat, but also clear blood toxin. It is used for various febrile diseases, such as body heat, rash, spots, heat-toxin sores, sore throat, etc., and the effect is remarkable.

Specific embodiment 1:

The curculigo is extracted first.

Then use the other raw materials Digupi, Atractylodes macrocephala, Rehmannia glutinosa, Rhodiola rosea, Epimedium, Panax notoginseng, Astragalus, Jiaogulan, Schisandra chinensis, Albizia juniper, raspberry, Poria cocos, Zhishouwu, mulberry, wolfberry, eyebright, yam, Achyranthes bidentata, Radix Paeoniae Rubra, Corydalis Corydalis, Honeysuckle, extracted and concentrated to make granule, powder or ointment.

The prepared extract granules of Cornus officinalis and other raw materials are sterilized by ultraviolet rays and packed in bags, and can be directly washed with boiled water when used.

Specific embodiment 2:

About 5000g of curculigo collected, chopped, soaked and placed in a stainless steel bucket with a cover, added 70% ethanol solution, the mass is 0.8-1 times of coarse particles, stirred evenly, kept moist and airtight for more than 1 hour, so that fully Expansion: Wrap the filter plate at the bottom of the percolation cylinder with a gauze bag and pave it flat, then mix and loosen the wet and expanded drug samples, then use a stainless steel spoon to fill the powder, and put it evenly into the percolation cylinder, with a thickness of 10-12 cm , press evenly with a T-shaped rod, and then follow the above operation, load layer by layer, pressurize appropriately, the powder filling should not exceed 2/3 of the height of the percolation cylinder; the powder surface is covered with a stainless steel orifice plate and pressed firmly, Open the discharge valve under the percolation cylinder, put a container, and then slowly add 70% ethanol liquid; after the air between the powder particles is discharged, and about 20L of ethanol flows out, close the discharge valve and cover the filter After soaking for 24 hours, open the material valve for percolation, control the percolation speed, generally 1000g of medicinal materials flow out 2-3ml per minute, put the filtrate into the liquid storage tank, and pour the ethanol solution when emptying into the liquid storage tank ; Combine the leachate and let it stand for later use as component 1.

The remaining raw materials are 1500g Digupi, 1500g Atractylodes macrocephala, 1500g Rehmannia glutinosa, 1500g Rhodiola, 1500g Epimedium, 1500g Sanqi, 1500g Astragalus, 1500g Jiaogulan, 1500g Schisandra, 1500g Albizia Jubilata, 1500g raspberry, 1500g Poria cocos. 1500g blackberry, 1500g mulberry, 1500g wolfberry, 1500g eyebright, 1500g yam, 1500g achyranthes bidentata, 1500g red peony root, 1500g corydalis corydalis, 1500g honeysuckle flower, washed to remove impurities, soaked in ethanol, heated and refluxed twice, 1 to 2 hours each time , combine the two extracts and let stand; combine the above two extracts, recover ethanol under reduced pressure and concentrate until the concentration of the medicinal solution is 0.6g crude drug/mL, after suction filtration, the relative density of the filtrate is about 1.08 at 20°C; Reduce the pressure to 0.03-0.08MPa, keep the temperature at 60-80°C, concentrate to a relative density of 1.20, and extract the extract at a temperature of 60°C-70°C as component 2.

Put components 1 and 2 into a double-effect vacuum concentrator, concentrate to a concentrated solution with a relative density of 1.05 at 90°C, and refrigerate at a low temperature of 0-5°C for 24 hours; add 0.3% filter aid to the refrigerated solution Diatomaceous earth, filter, and put the filtrate into a double-effect vacuum concentrator, concentrate to contain 0.1g of crude drug per 1ml, and then add dextrin to make an oral liquid.

Specific embodiment 3:

About 4000g of curculigo collected, chopped, soaked in a stainless steel bucket with a cover, added 70% ethanol solution, the mass is 0.8-1 times that of coarse particles, stirred evenly, kept moist and airtight for more than 1 hour, so that it can fully expand Wrap the filter plate at the bottom of the percolation cylinder with a gauze bag and spread it flat, then mix and loosen the wet and swelled drug sample, then use a stainless steel spoon to fill the powder, and put it evenly into the percolation cylinder, with a thickness of 10-12 cm. Use a T-shaped rod to press evenly, and then follow the above operation, load layer by layer, pressurize appropriately, the powder filling should not exceed 2/3 of the height of the percolation cylinder; the surface of the powder is covered with a stainless steel orifice plate, press firmly, open Put the discharge valve under the percolation cylinder, and put a container, and then slowly add 70% ethanol liquid; after the air between the powder particles is discharged, and about 20L of ethanol flows out, close the discharge valve and cover the cylinder , Immerse for 24 hours, then open the material valve for percolation, control the percolation speed, generally 1000g medicinal materials flow out 2-3ml per minute, put the filtrate into the liquid storage tank, and pour the ethanol liquid when emptying into the liquid storage tank; Combine the leachate and let it stand for later use as component 1; the remaining raw materials Digupi 1200g, Atractylodes macrocephala 1200g, Rehmannia glutinosa 1300g, Rhodiola rosea 1200g, Epimedium 1300g, Panax notoginseng 1200g, Astragalus 1100g, Jiaogulan 1100g, 1200g schisandra, 1100g acacia, 1200g raspberry, 1300g poria cocos, 1200g mulberry, 1200g wolfberry, 1200g eyebright, 1200g yam, 1200g achyranthes, 1100g red peony, 1100g corydalis, 1200g honeysuckle, put them in Soak in 10 times the amount of water. Put it into a multi-functional extraction tank to extract twice, add 8-10 times the amount of water to decoct for 1-2 hours for the first time, add 4-8 times the amount of water to decoct for 1-2 hours for the second time, combine the two decoctions, and filter Obtain filtrate; heat and concentrate to paste, add honey to reconcile the preparation.

Pharmacological toxicity test

Experimental example 1: acute toxicity test of the present invention

1. Test materials: Animals: Kunming mice, weighing 18-25g, half male and half male, bred by the biological laboratory of Shandong University. Medicine: the present invention (all raw materials are mixed and decocted twice, combined and filtered, and the medicinal solution is taken) contains 0.0365 mg/ml.

2. Method:

1. Calculation of LD50: adopt the modified Cole's method, mice are randomly divided into 5 groups, 10 in every group, half male and half male, the present invention is dissolved in distilled water, made into maximum concentration, and administered according to the maximum allowable capacity of mice. Dosing is followed by 18, 14.4, 11.5, 9.2, 7.4 (g.kg-1) according to the crude drug amount, and after the animal is fasted (can't help water) for 18 hours, it is divided into two administrations (interval half an hour) in one day, 0.5ml each time, observe the animal death.

2. Determination of maximum tolerated dose (MTD value): take 20 mice, 10 males and 10 males. The present invention is added with distilled water and dissolved to make the highest concentration. According to the maximum tolerance of the animal, the injection feeding device can twitch as the criterion. After the animals fasted (without water) for 18 hours, they were administered twice a day (with an interval of half an hour), 0.5ml each time (each ml contained 0.36g of crude drug), and the total drug dose was 18g of crude drug/kg.d, equivalent to 300 times the amount of clinical adult 50Kg body weight. Continuous observation for 7 days after administration.

3. Test results:

In the LD50 calculation, when the mice were administered with the maximum allowable concentration and the maximum allowable capacity (18g/Kg.d), no death of the mice was seen, that is, the LD50 was not measured, and only the maximum tolerated dose could be obtained. During the 7-day observation period , the animals' appetite, activity, coat color, mental state, etc. were all normal, and the development was normal, and no death was seen. That is, the dosage equivalent to 300 times of the clinical dose was selected, and no adverse reaction occurred, indicating that the acute toxicity was extremely small, and the MTD was greater than 18g/Kg.d.

Experimental example 2: Acute toxicity and long-term toxicity test results

Acute toxicity test: give the present invention by one-time gavage of mice, maximum concentration 35%, maximum gavage capacity 0.4ml/10g, dose 14g/kg (every g of powder is equivalent to 10g crude drug), observe continuously for 7 days, no For any toxic reaction, the LD 50 of the drug could not be measured because the concentration and dose could not be increased. Determination of the maximum tolerated dose: with the highest concentration and the maximum gavage volume, the mice were gavaged three times with an interval of 5 hours, and then observed continuously for 7 days, and no case died. The dosage of the medicinal powder is >42g/kg.day (each g of medicinal powder is equivalent to 10g of crude drug), which is equivalent to 420 times of the clinical daily dosage of an adult in terms of kilogram body weight.

Long-term toxicity test: in order to observe whether long-term medication produces toxic reactions, give rats the present invention to feed respectively, by 70 times and 35 times (being 7g/kg/ day and 3.5g/kg/ day) of adult's clinical daily dose, continuous After feeding for 8 weeks, there was no abnormality in the behavior, eating, and body weight of the rats. Compared with the control group, the blood routine, liver and kidney functions, and various important organs had no abnormal changes. Any toxic and side effects of the invention. Acute and chronic toxicity tests on animals prove that the invention has a large safety range and is a safe and reliable health care product.

Pharmacodynamics experiment:

Using modern immunology theory and technology to study the effect of traditional Chinese medicine on enhancing and regulating the immune function of the body is of great significance for clarifying the material basis of the theory of traditional Chinese medicine. The invention is formed by scientific compatibility of several natural medicines such as cornel officinalis, codonopsis pilosula and astragalus. Existing studies have found that Cornus officinalis can adjust the functions of various organs of the body, and has a regulatory effect on immune cells and immune molecules. This experiment aims to further explore the molecular immunological mechanism of the present invention's immunoregulatory effect on immunocompromised mice.

1 Materials and methods

1.1 Instruments and reagents: Curculigo curculigo Oral Liquid of the present invention is provided by the hospital (prepared according to the method in Example 2); cyclophosphamide for injection is provided by Shanghai Hualian Pharmaceutical Co., Ltd. Cytokine detection kits were purchased from American R&D Company. Flow cytometer, 2000FCA, produced by BD Company of the United States.

1.2 Animals Thirty Kunming male mice, 5-7 weeks old, weighing 25±2g, were provided by Shandong University Experimental Animal Co., Ltd.

1.3 Method

1.3.1 Establishment of animal model and grouping Kunming male mice were randomly divided into 3 groups: blank control group, model group and drug group, with 10 mice in each group. Blank control group: normal feeding without any treatment during the experiment; model group: intraperitoneal injection of 100 mg/kg cyclophosphamide to cause hypoimmunity model, once a day, for 3 consecutive days; drug group: intraperitoneal injection of 100 mg/kg cyclophosphamide Phosphoramide induced hypoimmunity model, 1 time/d, for 3 consecutive days, and smeared on the left tibialis anterior muscle of mice on the first day of modeling, 0.1mL/mouse, boosted immunization once a week later, the dose was the same as that of the first day once. Samples were collected after 4 days.

1.3.2 Determination of immune organ index in mice After the mice were weighed, the mice were killed by cervical dislocation, and the thymus and spleen were taken to weigh their wet weight, and the thymus index and spleen index were calculated: thymus index=thymus weight×1000/body weight and spleen index =spleen weight×1000/body weight 1.3.3 Determination of IgG and IgM levels in mouse serum According to the kit instructions, the IgG and IgM levels in mouse serum were measured.

1.3.4 Determination of T lymphocytes and their subsets CD4+, CD8+ and CD4+/CD8+ in mouse spleen tissue Aseptically take the spleen, place it in a plate containing an appropriate amount of Hank's-free liquid, and gently grind the spleen with tweezers to prepare A single cell suspension was filtered through a 200-mesh sieve, the cells were suspended in 1 mL of complete culture medium, and the cell concentration was adjusted to 1×10 ⁹ L ^-1 , and 100 μL of well-mixed splenocytes were carefully suspended with a micropipette. Add the solution to the bottom of the injection tube, add 20 μL of monoclonal antibody, oscillate at low speed for 3 seconds, incubate at room temperature in the dark for 30 minutes, break red blood cells in The medicine studyPREP, and detect the content of T cell subsets on a flow cytometer.

1.3.5 Determination of IFN-Y and IL-10 levels in mouse serum According to the kit instructions, the levels of IFN-Y and IL-10 in serum were measured.

1.2 Statistical processing All values are represented by x ± s, and SPSS13.0 statistical software is used for data analysis, and t test is used for comparison between groups. Take P<0.05 as the standard of statistically significant difference.

2 results

2.1 Effects on the immune organs of the immunocompromised mice It can be concluded from Table 1 that compared with the normal control group, the spleen and thymus indexes in the model group were significantly lower, and the difference was statistically significant, P<0.01. Compared with the model group, the spleen index of the mice in the drug group increased significantly, which was statistically significant, P<0.01, and the thymus index also increased, but the difference was not significant.

Table 1 The present invention is to the influence (n=10, mean ± s) of immunocompromised mice immune organ weight

group Spleen index (mg/g) Thymus index (mg/g) normal control group 6.02±1.04 3.23±1.01 model group 7.35±0.89△△ 1.43±0.75△△ Invention group 5.45±0.42** 1.08±0.86

Compared with the normal control group: △: P<0.05, △△: P<0.01; compared with the model group: *: P<0.05, **: P<0.01

2.2 Effects on T lymphoid subgroups in spleen tissue of immunocompromised mice It can be seen from Table 2 that compared with the normal control group, the percentage of CD4+ cell subsets in the spleen of the model group mice decreased, and the percentage of CD8+ cell subsets increased, with significant differences , P<0.05, the ratio of CD4+/CD8+ decreased significantly, with a very significant difference, P<0.01. However, the drug group can obviously counteract the effect of cyclophosphamide on destroying immunity, with a significant difference, P<0.05.

Table 2 The effect of King Kong Tiandun Liquid on humoral T cell subsets in immunocompromised mice (n=10, mean±SD)

Compared with the normal control group: △P<0.05, △△p<0.05; compared with the model group: *P<0.05, **P<0.05.

Thymus and spleen are important lymphatic organs of the body. The thymus is the place where T cells develop, differentiate and mature, and its structure and function are closely related to the state of the body. The reduction of thymus index and thymus atrophy are the manifestations of immunosuppression mainly of cellular immunity in mice. The spleen is the largest peripheral immune organ in the body. The immune cells of the spleen are mainly B lymphocytes, and B lymphocytes are the main cells involved in humoral immunity. Therefore, the spleen is closely related to the humoral immunity of the body. Thymus index and spleen index intuitively reflect the strength of immune body function. This experiment shows that the invention can promote the development of the spleen of mice with hypoimmunity induced by cyclophosphamide, and can also promote the development of the thymus to a certain extent. T lymphocytes can be divided into two subgroups, CD4+ and CD8+, according to their functions. CD4+ T lymphocytes play a very important central role in the body's immune response. It activates other T and B cell subsets and other cells participating in the immune response by secreting a variety of lymphokines, making them proliferate and differentiate, and coordinate the immune response. The interaction between cells plays a role in the body's immune system. An increase in the ratio of CD4+/CD8+ indicates that the positive regulation of the immune response is dominant, while a decrease in the ratio indicates that the immune function is low. The determination of T lymphocyte subsets in peripheral blood shows that the present invention can increase the percentage content of CD4+T lymphocytes in immunocompromised mice caused by cyclophosphamide, and increase the ratio of CD4+/CD8+, suggesting that the present invention Play a positive regulatory role on the T lymphocyte immune system. IgG, IgM levels reflect the humoral immunity. This study shows that the health-care tea of Cornus officinalis can promote the secretion of IgG and IgM in mice with low immunity. It shows that the present invention not only promotes the enhancement of the body's cellular immune function, but also promotes the humoral immunity.

Claims (10)

1. the preparation method of the Rhizoma Curculiginis oral liquid improving immunity, it is characterised in that the preparation process bag of described oral liquid Include:

A. the extraction of Rhizoma Curculiginis, as component 1；

B. remaining crude drug described is put into heating and refluxing extraction 2 times in ethanol, as component 2；

C. by 2-in-1 also to above two extracting solution component 1 and component, add dextrin after concentration and be in harmonious proportion, be prepared as after disinfection being administered orally Liquor.

Improve the preparation method of the Rhizoma Curculiginis oral liquid of immunity the most according to claim 1, it is characterised in that described step a In, the Rhizoma Curculiginis chopping that will gather, to be soaked and be placed in lid stainless steel cask, added 70% ethanol, quality is coarse grained 0.8-1 times, stir, moistening airtight placement more than 1 hour, make fully to expand；By the gauze bag bag of filter plate bottom percolator Wrap up in again the drug sample after moistening expansion is mixed after paving pine do dissipate, then with rustless steel spoon contain powder, load percolator uniformly, Dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitably pressurize, medicated powder must not load and surpassed Cross 2/3 eminence of percolator；Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, Then 70% ethanol it is slowly added into；Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, closedown is put Material valve, covers cylinder of filtering, dipping 24 hours, then opens baiting valve and carries out percolation, controls percolation speed and is generally 1000g medical material every point Clock flows out 2～3ml, and filtrate puts into liquid container, and pours ethanol during emptying into liquid container；Percolate is merged and stands, Stand for standby use, as component 1.

Improve the preparation method of the Rhizoma Curculiginis oral liquid of immunity the most according to claim 1, it is characterised in that described step b In, remaining raw material is steeped in 10 times amount ethanol after 10-15 days, heating extraction 2 times, each 1-2 hour, remove supernatant, merge Extracting solution, 100-110 mesh filters, then the ultrafiltration post ultrafiltration that molecular weight is 6000-10000 through damming, ultrafiltrate concentrating under reduced pressure phase The extractum of 1.36 when being 80 DEG C to density, heating is concentrated into paste, stand for standby use, becomes component 2.

Improve the preparation method of the Rhizoma Curculiginis oral liquid of immunity the most according to claim 1, it is characterised in that described step c In, the extracting solution that above-mentioned steps is extracted, put in economic benefits and social benefits vacuum concentrator and mix, first concentrate the filtrate to when 80 DEG C the closeest Degree is the concentrated solution of 1.32, sets to 0～5 DEG C of deepfreezes 24 hours；The filter aid kieselguhr adding 0.3% by cold preservation liquid, filters, filter Liquid is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml extractum Han 0.1g, adds dextrin and is in harmonious proportion, is prepared as mouth after disinfection Take liquor.

5. the preparation method of the Rhizoma Curculiginis oral liquid improving immunity, it is characterised in that its preparation process is:

A. the preparation of Rhizoma Curculiginis, as component 1；

B. remaining crude drug described chopping is extracted as component 2；

C. component 1 and component 2 are mixed, add proper honey after concentration and be in harmonious proportion, bottle after ultraviolet ray sterilization bactericidal.

Improve the preparation method of the Rhizoma Curculiginis oral liquid of immunity the most according to claim 5, it is characterised in that described step a In, the Rhizoma Curculiginis of collection is put in 5-10 times amount ethanol, soak 1-2 hour, heating extraction 2 times, each 1-2 hour, remove supernatant Liquid, united extraction liquid, 100-120 mesh filters；The medicinal residues of other crude drug crossed by above-mentioned ethanol extraction again add 10 times amount water and add 2 extracting solution each 1～2 hour, are merged and stand by circumfluence distillation 2 times；Above two extracting solution is merged, recovered under reduced pressure Ethanol, and to be concentrated into liquor strength be 0.6g crude drug/mL, after sucking filtration, when the relative density of filtrate is about 20 DEG C 1.08；It is decompressed to 0.03-0.08MPa, temperature is maintained at 60-80 DEG C, and being concentrated into relative density is 1.20, the extractum of temperature to 60 DEG C-70 DEG C, for Component 1.

Improve the preparation method of the Rhizoma Curculiginis oral liquid of immunity the most according to claim 5, it is characterised in that described step b In, remaining raw medicinal material is mixed, shredded 100～120 mesh sieves, after sieving, fine powder is standby, and coarse granule puts into acid and alkali-resistance dipping Pot, at room temperature, impregnates 15～30 days, then heating and refluxing extraction 2 times, each 1～2 hour together with more than 70 degree ethanol, 2 extracting solution are merged and stands, filter, after separation, take filtrate；By filtered through gauze, residue adds 50-60% ethanol, 60 DEG C- 70 DEG C are continued extraction 2h, every 10min and stir once, and filtered through gauze merges lixiviating solution, and being condensed into pasty state becomes component 2.

8. the Rhizoma Curculiginis oral liquid improving immunity, it is characterised in that described oral liquid includes the raw material of following weight portion: celestial Thatch 10～50 parts, Cortex Lycii 10～20 parts, the Rhizoma Atractylodis Macrocephalae 10～20 parts, Radix Rehmanniae Preparata 10～20 parts, Radix Rhodiolae 10～20 parts, Herba Epimedii 10～ 20 parts, Radix Notoginseng 10～20 parts, the Radix Astragali 10～20 parts, Herb Gynostemmae Pentaphylli 10～20 parts, Fructus Schisandrae Chinensis 10～20 parts, Cortex Albiziae 10～20 parts, Fructus Rubi 10～20 parts, Poria 10～20 parts, Radix Polygoni Multiflori Preparata 10～20 parts, Fructus Mori 10～20 parts, Fructus Lycii 10～20 parts, Herba Euphrasiae Regelii 10 ～20 parts, Rhizoma Dioscoreae 10～20 parts, Radix Achyranthis Bidentatae 10～20 parts, Radix Paeoniae Rubra 10～20 parts, Rhizoma Corydalis 10～20 parts, Flos Lonicerae 10～20 parts.

Improve the Rhizoma Curculiginis oral liquid of immunity the most according to claim 8, it is characterised in that described oral liquid includes following heavy The raw material of amount part: Rhizoma Curculiginis 10～40 parts, Cortex Lycii 10～15 parts, the Rhizoma Atractylodis Macrocephalae 10～15 parts, Radix Rehmanniae Preparata 10～15 parts, Radix Rhodiolae 10～15 Part, Herba Epimedii 10～15 parts, Radix Notoginseng 10～15 parts, the Radix Astragali 10～15 parts, Herb Gynostemmae Pentaphylli 10～15 parts, Fructus Schisandrae Chinensis 10～15 parts, close Joyous skin 10～15 parts, Fructus Rubi 10～15 parts, Poria 10～15 parts, Radix Polygoni Multiflori Preparata 10～20 parts, Fructus Mori 10～20 parts, Fructus Lycii 10～ 20 parts, Herba Euphrasiae Regelii 10～20 parts, Rhizoma Dioscoreae 10～20 parts, Radix Achyranthis Bidentatae 10～20 parts, Radix Paeoniae Rubra 10～20 parts, Rhizoma Corydalis 10～20 parts, gold Flos Lonicerae 10～20 parts.

Improve the Rhizoma Curculiginis oral liquid of immunity the most according to claim 8, it is characterised in that described oral liquid includes following The raw material of weight portion: Rhizoma Curculiginis 10～50 parts, Cortex Lycii 10～20 parts, the Rhizoma Atractylodis Macrocephalae 10～20 parts, Radix Rehmanniae Preparata 10～20 parts, Radix Rhodiolae 10～ 20 parts, Herba Epimedii 10～20 parts, Radix Notoginseng 10～20 parts, the Radix Astragali 10～20 parts, Herb Gynostemmae Pentaphylli 10～20 parts, Fructus Schisandrae Chinensis 10～20 parts, Cortex Albiziae 10～20 parts, Fructus Rubi 10～20 parts, Poria 10～20 parts, Radix Polygoni Multiflori Preparata 10～15 parts, Fructus Mori 10～15 parts, Fructus Lycii 10 ～15 parts, Herba Euphrasiae Regelii 10～15 parts, Rhizoma Dioscoreae 10～15 parts, Radix Achyranthis Bidentatae 10～15 parts, Radix Paeoniae Rubra 10～15 parts, Rhizoma Corydalis 10～15 parts, Flos Lonicerae 10～15 parts.