CN106117298A - Molecular engram extracts the way of Ganodenic acid with ultrasonic coupling - Google Patents
Molecular engram extracts the way of Ganodenic acid with ultrasonic coupling Download PDFInfo
- Publication number
- CN106117298A CN106117298A CN201610501817.6A CN201610501817A CN106117298A CN 106117298 A CN106117298 A CN 106117298A CN 201610501817 A CN201610501817 A CN 201610501817A CN 106117298 A CN106117298 A CN 106117298A
- Authority
- CN
- China
- Prior art keywords
- molecular engram
- ganodenic acid
- extracts
- extracting
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses the way of a kind of molecular engram and ultrasonic coupling extraction Ganodenic acid, relate to the extraction and separation technology field of natural product.It is using REISH as extracting raw material that this molecular engram and ultrasonic coupling extract the way of Ganodenic acid, using water as extracting medium, utilize the Ganodenic acid in molecular engram material adsorbing and extracting liquid, from extracting solution, the molecular engram material being adsorbed with Ganodenic acid is extracted again with ultrasonic extraction device, after extraction, the Ganodenic acid of absorption on molecular engram material described in eluting.The present invention solves the technical problem that the extracting method of current Ganodenic acid exists that environmental pollution is big, yield is low, purity is low etc..
Description
Technical field
The present invention relates to the extraction and separation technology field of natural product, especially a kind of molecular engram extracts with ultrasonic coupling
The way of Ganodenic acid.
Background technology
Ganoderma is On Polyporaceae Ganoderma also known as Lin Zhongling, fine jade precious (formal name used at school: Ganoderma Lucidum Karst)
Sporophore.Ganoderma contains multiple chemical composition, and its main active has polysaccharide and peptide polysaccharide class, alkaloids and nucleotide
Class, amino acid and protein class, triterpenes, trace element.This triterpenoid compound has hepatoprotective effect, antitumor action, resists
HIV1 and HIV2 proteinase activity, suppression histamine release, Angiotensin-converting enzyme inhibition (ACE), suppression cholesterol biosynthesis, battle array
Pain effect, the impact on platelet aggregation, the inhibitory action to albumen Acacia farnesiana Willd. transesterify enzyme, antioxidation etc..And Ganoderma
The important activity composition of triterpenoid compound is exactly Ganodenic acid, and the extracting method of current Ganodenic acid is often molten as extracting with chloroform
Agent obtains through silica gel purification repeatedly, there is the technical problems such as environmental pollution is big, yield is low, purity is low.
Molecular imprinting is the focus of research at present, and it has structure effect precordainment, the feature of specific recognition, is a kind of
Relatively there is the technology of industrialization value.This technology is usually target molecule as template molecule, selects in sense unity
Function monomer matched on structure, allows and covalent effect or noncovalent interaction occurs between the two, adds cross-linking agent by heat
Polymerization or photopolymerization, produce polyreaction under the induction of the porogen of initiator, thus form the height being enclosed with target molecule
Polymers, i.e. molecularly imprinted polymer (MIPs), subsequently, utilize physics or chemical method, by target molecule from polymeric inner
It is eluted out, once obtains and there is identical with target molecule shape and that position of functional group is certain memory hole.
Summary of the invention
The present invention provides a kind of molecular engram to extract the way of Ganodenic acid with ultrasonic coupling, and it can solve current Ganodenic acid
The extracting method technical problem that has that environmental pollution is big, yield is low, purity is low etc..
In order to solve the problems referred to above, the technical solution adopted in the present invention is: this molecular engram extracts with ultrasonic coupling
The way of Ganodenic acid is using REISH as extracting raw material, using water as extracting medium, utilizes molecular engram material absorption to carry
Take the Ganodenic acid in liquid, then from extracting solution, extract the molecular engram material being adsorbed with Ganodenic acid with ultrasonic extraction device, extract
After, the Ganodenic acid of absorption on molecular engram material described in eluting；
Described molecular engram material comprises the following steps:
A, being pulverized by dry Ganoderma, the Ganoderma powder quality of gained extracts 3 times than the ethanol water that concentration is 80%,
Merging the lixiviating solution of 3 gained, controlling temperature in each leaching process is 60 DEG C, and each time is 5h；
The consumption of described REISH and the consumption of described ethanol water, by described REISH 1kg, described ethanol water
The ratio of 5～10L calculates；
B, the lixiviating solution of step A gained is concentrated to give Ganodenic acid；
C, 1～1.5mmol Ganodenic acid and 6mmol methacrylic acid are dissolved in 5～10ml chloroforms, are placed at 0 DEG C holding
30min, is subsequently adding 15～30mmol Ethylene glycol dimethacrylate and 15～30mmolN-N-isopropylacrylamides and 40
～100mg azodiisobutyronitrile, after mixing, it being passed through nitrogen, stirring is to being completely dissolved, and logical nitrogen 10min, sealing, at 65 DEG C of bars
Part reaction, until polymerization completely, is pulverized, is crossed 200 mesh sieves, is the methanol of 9:1 by volume ratio: alcohol mixeding liquid eluting Ganodenic acid, i.e.
Available molecular engram material.
In technique scheme, more specifically technical scheme is it may also is that described supersound extraction process control is at 250W,
40MHz。
Further: after extraction, first adsorb in institute with 50 DEG C of water elutions of 60 times of described molecular engram material weight
State the impurity on molecular engram material, more i.e. can get Ganodenic acid with 25 DEG C of water elutions of 30 times of described molecular engram material weight
Solution.
Owing to have employed technique scheme, the present invention compared with prior art has the advantages that
1, the way technique environmental protection extracting Ganodenic acid of the present invention, does not use any organic reagent, i.e. improves supersound extraction
Efficiency, is readily available again the Ganodenic acid aqueous solution that purity is higher.
2, the lowest critical solution temperature (LCST) of imprinted material prepared by the present invention is about 38 DEG C, supersound extraction because
Meeting accumulated heat, temperature easily reaches more than 38 DEG C, without external heat source, energy-conserving and environment-protective.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described:
The way that this molecular engram extracts Ganodenic acid with ultrasonic coupling first has to prepare molecular engram, and preparation method is as follows:
A, the Ganoderma being dried by 1kg are pulverized, and the Ganoderma powder 5L mass of gained is than the ethanol water extraction that concentration is 80%
3 times, merging the lixiviating solution of 3 gained, controlling temperature in each leaching process is 60 DEG C, and each time is 5h；
B, the lixiviating solution of step A gained is concentrated to give Ganodenic acid；
C, 1mmol Ganodenic acid and 6mmol methacrylic acid are dissolved in 5ml chloroform, are placed at 0 DEG C holding 30min, then add
Enter 15mmol Ethylene glycol dimethacrylate and 15mmolN-N-isopropylacrylamide and 40mg azodiisobutyronitrile, mixing
After, it being passed through nitrogen, stirring, to being completely dissolved, is led to nitrogen 10min, sealing, at 65 DEG C of conditioned responses until polymerization is complete, is pulverized,
Cross 200 mesh sieves, be the methanol of 9:1 by volume ratio: alcohol mixeding liquid eluting Ganodenic acid, i.e. can get molecular engram material.
Then prepared molecular engram material and ultrasonic coupling are carried out the extraction of Ganodenic acid, extract process as follows:
Using REISH as extracting raw material, using water as extracting medium, utilize the molecular engram material adsorbing and extracting liquid prepared
In Ganodenic acid, then from extracting solution, extract the molecular engram material being adsorbed with Ganodenic acid with ultrasonic extraction device, extract process
Control at 250W, 40MHz.After extraction, first adsorb in institute with 50 DEG C of water elutions of 60 times of described molecular engram material weight
State the impurity on molecular engram material, more i.e. can get Ganodenic acid with 25 DEG C of water elutions of 30 times of described molecular engram material weight
Aqueous solution.
This molecular engram material is reusable.
Claims (3)
1. a molecular engram and ultrasonic coupling extract the way of Ganodenic acid, it is characterised in that: former using REISH as extracting
Material, using water as extracting medium, utilizes the Ganodenic acid in molecular engram material adsorbing and extracting liquid, then with ultrasonic extraction device from carrying
Take and liquid extracts the molecular engram material being adsorbed with Ganodenic acid, after extraction, absorption on molecular engram material described in eluting
Ganodenic acid；
Described molecular engram material comprises the following steps:
A, being pulverized by dry Ganoderma, the Ganoderma powder quality of gained extracts 3 times than the ethanol water that concentration is 80%,
Merging the lixiviating solution of 3 gained, controlling temperature in each leaching process is 60 DEG C, and each time is 5h；
The consumption of described REISH and the consumption of described ethanol water, by described REISH 1kg, described ethanol water
The ratio of 5～10L calculates；
B, the lixiviating solution of step A gained is concentrated to give Ganodenic acid；
C, 1～1.5mmol Ganodenic acid and 6mmol methacrylic acid are dissolved in 5～10ml chloroforms, are placed at 0 DEG C holding
30min, is subsequently adding 15～30mmol Ethylene glycol dimethacrylate and 15～30mmolN-N-isopropylacrylamides and 40
～100mg azodiisobutyronitrile, after mixing, it being passed through nitrogen, stirring is to being completely dissolved, and logical nitrogen 10min, sealing, at 65 DEG C of bars
Part reaction, until polymerization completely, is pulverized, is crossed 200 mesh sieves, is the methanol of 9:1 by volume ratio: alcohol mixeding liquid eluting Ganodenic acid, i.e.
Available molecular engram material.
Molecular engram the most according to claim 1 extracts the way of Ganodenic acid with ultrasonic coupling, it is characterised in that: described super
Sound extracts process control at 250W, 40MHz.
Molecular engram the most according to claim 1 extracts the way of Ganodenic acid with ultrasonic coupling, it is characterised in that: extract
Bi Hou, first adsorbs the impurity on described molecular engram material with 50 DEG C of water elutions of 60 times of described molecular engram material weight,
Ganodenic acid solution is i.e. can get again with 25 DEG C of water elutions of 30 times of described molecular engram material weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610501817.6A CN106117298A (en) | 2016-06-30 | 2016-06-30 | Molecular engram extracts the way of Ganodenic acid with ultrasonic coupling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610501817.6A CN106117298A (en) | 2016-06-30 | 2016-06-30 | Molecular engram extracts the way of Ganodenic acid with ultrasonic coupling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106117298A true CN106117298A (en) | 2016-11-16 |
Family
ID=57285628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610501817.6A Pending CN106117298A (en) | 2016-06-30 | 2016-06-30 | Molecular engram extracts the way of Ganodenic acid with ultrasonic coupling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106117298A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106946966A (en) * | 2017-04-29 | 2017-07-14 | 北京林业大学 | A kind of subcritical water law extracts wild red ganoderma ganoderic acid A and C simultaneously2Method |
| CN111574347A (en) * | 2020-05-27 | 2020-08-25 | 广西大学 | A molecular imprinting method for extracting jasmonone and jasmonolide from jasmine slag |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1181271A (en) * | 1996-10-31 | 1998-05-13 | 高益槐 | Water extracting process for high activity glossy ganoderma holoside and high content glossy ganoderma acid |
| CN103664942A (en) * | 2013-12-16 | 2014-03-26 | 广西大学 | Method for extracting matrine by coupling molecular imprinting with ultrasonic extraction |
-
2016
- 2016-06-30 CN CN201610501817.6A patent/CN106117298A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1181271A (en) * | 1996-10-31 | 1998-05-13 | 高益槐 | Water extracting process for high activity glossy ganoderma holoside and high content glossy ganoderma acid |
| CN103664942A (en) * | 2013-12-16 | 2014-03-26 | 广西大学 | Method for extracting matrine by coupling molecular imprinting with ultrasonic extraction |
Non-Patent Citations (1)
| Title |
|---|
| 乐康等: "丹参酮ⅡA分子印迹聚合物的制备及其丹参活性成分的分离", 《华西药学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106946966A (en) * | 2017-04-29 | 2017-07-14 | 北京林业大学 | A kind of subcritical water law extracts wild red ganoderma ganoderic acid A and C simultaneously2Method |
| CN111574347A (en) * | 2020-05-27 | 2020-08-25 | 广西大学 | A molecular imprinting method for extracting jasmonone and jasmonolide from jasmine slag |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104557893A (en) | Extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves | |
| CN101260131A (en) | Method for extracting iridoid active site and monomer from Eucommia ulmoides | |
| CN103172804B (en) | Preparation method of theophylline molecular surface printing material | |
| CN103408602B (en) | A kind of method being separated preparation four kinds of glycoside chemical reference substances from ZANGYINCHEN | |
| CN103664942B (en) | A kind of molecular imprinting and ultrasonic coupling extract the method for matrine | |
| CN102166512B (en) | Ginsenoside Rg1 molecularly imprinted material and its application | |
| CN101215302A (en) | A kind of preparation method of forsythiaside A | |
| CN107823237A (en) | A kind of extracting method of burdock root total flavone | |
| CN106117298A (en) | Molecular engram extracts the way of Ganodenic acid with ultrasonic coupling | |
| CN102617813B (en) | Preparation and application of sephadex surface apigenin molecular engram sorbing material | |
| CN100390211C (en) | The preparation method of highly polar polystyrene adsorption resin and its application in the separation of bologna alkaloids | |
| CN103408610B (en) | The method of arbutin is extracted from leaf of pear tree | |
| CN104130310A (en) | Separation and purification method for glutathione | |
| CN106117386A (en) | The method that molecular engram extracts ganoderan with ultrasonic coupling | |
| CN110117342B (en) | A kind of phenethyl alcohol glycoside molecularly imprinted polymer and its preparation method and application | |
| CN106146716A (en) | A kind of proanthocyanidin B2 molecularly imprinted polymer and its preparation method and application | |
| CN104189026B (en) | A kind of method for removing from Ginkgo Biloba Extract and being enriched with ginkgoic acid | |
| CN103224590A (en) | Glabridin molecularly imprinted polymer, as well as preparation method and application thereof | |
| CN106074629A (en) | The method that molecular engram extracts Ganoderma triterpenoids with ultrasonic coupling | |
| CN104072456A (en) | Preparation method of high-purity salvianolic acid B | |
| CN103739602A (en) | Method for extracting matrine | |
| CN105130793A (en) | Method of simultaneously extracting caffeic acid and ferulic acid in cimicifuga rhizome | |
| CN109893547B (en) | Method for extracting ganoderma triterpene and ganoderma polysaccharide from ganoderma | |
| CN104262451B (en) | A kind of method extracting selenka from Holothurian machining waste liquid | |
| CN105602926A (en) | Preparation method and application of topology isomerase II hybridized nanoflowers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161116 |
|
| WD01 | Invention patent application deemed withdrawn after publication |