CN106074669A - The extracting method of Fructus Momordicae polyphenol - Google Patents

The extracting method of Fructus Momordicae polyphenol Download PDF

Info

Publication number
CN106074669A
CN106074669A CN201610628239.2A CN201610628239A CN106074669A CN 106074669 A CN106074669 A CN 106074669A CN 201610628239 A CN201610628239 A CN 201610628239A CN 106074669 A CN106074669 A CN 106074669A
Authority
CN
China
Prior art keywords
extracting
drying
fructus momordicae
peel
ethanol solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610628239.2A
Other languages
Chinese (zh)
Inventor
何伟平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guilin Strength Science And Technology Ltd
Original Assignee
Guilin Strength Science And Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guilin Strength Science And Technology Ltd filed Critical Guilin Strength Science And Technology Ltd
Priority to CN201610628239.2A priority Critical patent/CN106074669A/en
Publication of CN106074669A publication Critical patent/CN106074669A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/42Cucurbitaceae (Cucumber family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/14Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1605Excipients; Inactive ingredients
    • A61K9/1629Organic macromolecular compounds
    • A61K9/1652Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/53Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/55Liquid-liquid separation; Phase separation

Abstract

The invention discloses the extracting method of a kind of Fructus Momordicae polyphenol, comprise the following steps: 1) take Fructus Momordicae peel, pulverize after microwave drying;2) drying and crushing peel addition ethanol solution will carry out ultrasonic extraction;3), after gained extracting solution is performing centrifugal separation on, after supernatant regulation to pH 3~4, upper polyamide resin column adsorbs, and first with the washing post of 10~15 DEG C, then is 55%~65% ethanol solution eluting by volumetric concentration;4), after eluent is concentrated in vacuo, mix with beta cyclodextrin, arabic gum, maltodextrin and soybean lecithin, homogenizing, be spray-dried, gained solid particle is product.Extraction ratio of the present invention is high, and products obtained therefrom storage performance is stable, purity is high, condition is good.

Description

The extracting method of Fructus Momordicae polyphenol
Technical field
The present invention relates to the preparation method of plant extract, having is the extracting method of a kind of Fructus Momordicae polyphenol.
Background technology
Fructus Momordicae, is commonly called as " angle fruit ", is the perennial liana of Cucurbitaceae.Its leaf is heart-shaped, and dioecism is opened summer Flower, seed in autumn.The traditional Chinese medical science, with its fruit medicine, containing medicinal ingredients such as mogroside, several amino acids and vitamin, cures mainly Lung-heat phlegm-fire cough, pharyngolaryngitis, tonsillitis, acute gastritis, constipation etc..In Fructus Momordicae containing various active composition such as flavone, Polysaccharide, sweet glycoside and polyphenol etc..Polyphenol components, as one of important functional components of Fructus Momordicae, mainly includes 5-hydroxyl Fructus Hordei Germinatus Phenol, kaempferol, magnolol etc., they can be removed body free radical, antibacterial, antiinflammatory, antitumor, can operate with food, medicine etc. Multiple fields.At present, the method extracting the sweet polyphenol of Fructus Momordicae has a lot, but often yield and purity are relatively low, is difficult to meet industry Demand.
Summary of the invention
The technical problem to be solved in the present invention is to provide the extracting method of a kind of Fructus Momordicae polyphenol, high with prepared yield, pure The Fructus Momordicae polyphenol extract spent.
For solve above-mentioned technical problem, the present invention by the following technical solutions:
The extracting method of Fructus Momordicae polyphenol, comprises the following steps: 1) take Fructus Momordicae peel, pulverizes, obtain dry after microwave drying Dry pulverizing peel;2) drying and crushing peel addition ethanol solution will carry out ultrasonic extraction;3) by gained extracting solution by centrifugation After separation, after supernatant regulation to pH 3~4, upper polyamide resin column adsorbs, and first with the washing post of 10~15 DEG C, then uses volume Concentration is 55%~65% ethanol solution eluting, collects eluent;4) after eluent is concentrated in vacuo, with beta-schardinger dextrin-, Arab Glue, maltodextrin and soybean lecithin mixing, homogenizing, be spray-dried, and gained solid particle is the many phenol extractions of Fructus Momordicae Thing.
In technique scheme:
Step 1) in, the mode of described microwave drying is: microwave heating 20~stopping heating 20~30 seconds after 30 seconds, circulation Carrying out 3~5 times, microwave intensity is 3W/g~10W/g.The granularity of described drying and crushing peel is preferably 60~120 mesh.
Step 2) in, the volumetric concentration of described ethanol solution is 65%~75%, and its addition is drying and crushing peel weight 10~20 times of amount;Described Extracting temperature is 50~55 DEG C, and extraction time is 0.5~1 hour.
Step 4) in, described eluent vacuum is preferably concentrated into the 35%~45% of original volume.Eluent after concentration with The weight ratio of beta-schardinger dextrin-, arabic gum, maltodextrin and soybean lecithin be 100:1~3:2~5:0.5~1:0.1~ 0.3。
The invention have the benefit that
1) use microwave drying, the activity of the enzymes such as the polyphenol oxidase being effectively passivated in arhat peel, put forward dry matter Amount, shortens drying time;2) utilize ultrasonic assistant to extract, shorten in extraction time to one hour, it is to avoid Polyphenols Compound partial reduction because extraction time is long, degraded;3) use polyamide resin column absorption to purify, first wash post with cold water, Use ethanol solution eluting again, can effectively go the removal of impurity, be avoided that again the loss of subject matter;4) spray drying is used to obtain granule Product, is dried front beta-schardinger dextrin-, arabic gum, maltodextrin and soybean lecithin mixing embedding Fructus Momordicae polyphenol, reduces sieve The Chinese fruit polyphenol product sensitivity to environment, strengthens its storage-stable;5) product extraction ratio is up to 2%~3%, and yield is high, Condition is good.
Detailed description of the invention
Below in conjunction with specific embodiment for instruction and explanation of the present invention.
Embodiment 1
The extracting method of Fructus Momordicae polyphenol, step is as follows:
1) take Fructus Momordicae peel, after microwave drying, be crushed to 60 mesh, obtain drying and crushing peel.The mode of microwave drying is: Microwave heating stops heating 25 seconds after 25 seconds, circulation carries out 5 times, and microwave intensity is 3W/g.
2) drying and crushing peel addition ethanol solution will carry out ultrasonic extraction.The volumetric concentration of ethanol solution is 70%, addition is 20 times of drying and crushing peel weight;Extracting temperature is 50 DEG C, and extraction time is 0.6 hour.
3), after being performing centrifugal separation on by gained extracting solution, after supernatant regulation to pH 4, upper polyamide resin column adsorbs, and first uses The washing post of 10 DEG C, then be 60% ethanol solution eluting by volumetric concentration, collect eluent.
4) eluent is concentrated in vacuo to the 45% of original volume, with beta-schardinger dextrin-, arabic gum, maltodextrin and Semen sojae atricolor ovum phosphorus Fat, mixes according to the weight ratio of 100:1:4:1:0.1, after homogenizing, is spray-dried, and it is many that gained solid particle is Fructus Momordicae Phenol extraction thing.
Embodiment 2
The extracting method of Fructus Momordicae polyphenol, step is as follows:
1) take Fructus Momordicae peel, after microwave drying, be crushed to 100 mesh, obtain drying and crushing peel.The mode of microwave drying For: microwave heating stops heating 30 seconds after 30 seconds, circulation carries out 3 times, and microwave intensity is 8W/g.
2) drying and crushing peel addition ethanol solution will carry out ultrasonic extraction.The volumetric concentration of ethanol solution is 75%, addition is 10 times of drying and crushing peel weight;Extracting temperature is 53 DEG C, and extraction time is 1 hour.
3), after being performing centrifugal separation on by gained extracting solution, after supernatant regulation to pH 3, upper polyamide resin column adsorbs, and first uses The washing post of 13 DEG C, then with volumetric concentration 65% ethanol solution eluting, collect eluent.
4) eluent is concentrated in vacuo to the 35% of original volume, with beta-schardinger dextrin-, arabic gum, maltodextrin and Semen sojae atricolor ovum phosphorus Fat, mixes according to the weight ratio of 100:2:5:0.5:0.2, after homogenizing, is spray-dried, and gained solid particle is Fructus Momordicae Polyphenol extract.
Embodiment 3
The extracting method of Fructus Momordicae polyphenol, step is as follows:
1) take Fructus Momordicae peel, after microwave drying, be crushed to 120 mesh, obtain drying and crushing peel.The mode of microwave drying For: microwave heating stops heating 20 seconds after 20 seconds, circulation carries out 4 times, and microwave intensity is 10W/g.
2) drying and crushing peel addition ethanol solution will carry out ultrasonic extraction.The volumetric concentration of ethanol solution is 65%, addition is 15 times of drying and crushing peel weight;Extracting temperature is 55 DEG C, and extraction time is 0.5 hour.
3), after being performing centrifugal separation on by gained extracting solution, after supernatant regulation to pH 3, upper polyamide resin column adsorbs, and first uses The washing post of 15 DEG C, then be 55% ethanol solution eluting by volumetric concentration, collect eluent.
4) eluent is concentrated in vacuo to the 40% of original volume, with beta-schardinger dextrin-, arabic gum, maltodextrin and Semen sojae atricolor ovum phosphorus Fat, mixes according to the weight ratio of 100:3:2:0.7:0.3, after homogenizing, is spray-dried, and gained solid particle is Fructus Momordicae Polyphenol extract.

Claims (6)

1. the extracting method of Fructus Momordicae polyphenol, it is characterised in that comprise the following steps: 1) take Fructus Momordicae peel, after microwave drying Pulverize, obtain drying and crushing peel;2) drying and crushing peel addition ethanol solution will carry out ultrasonic extraction;3) gained is extracted After liquid is performing centrifugal separation on, after supernatant regulation to pH 3~4, upper polyamide resin column adsorbs, first with the washing post of 10~15 DEG C, It is 55%~65% ethanol solution eluting by volumetric concentration again, collects eluent;4), after eluent is concentrated in vacuo, stick with paste with β-ring Essence, arabic gum, maltodextrin and soybean lecithin mixing, homogenizing, be spray-dried, and gained solid particle is Fructus Momordicae Polyphenol extract.
Extracting method the most according to claim 1, it is characterised in that the mode of described microwave drying is: microwave heating 20 ~after 30 seconds, stopping heating 20~30 seconds, circulation carries out 3~5 times, and microwave intensity is 3W/g~10W/g.
Extracting method the most according to claim 1, it is characterised in that: the granularity of described drying and crushing peel is 60~120 Mesh.
Extracting method the most according to claim 1, it is characterised in that: step 2) in, the volumetric concentration of described ethanol solution Being 65%~75%, its addition is 10~20 times of drying and crushing peel weight;Described Extracting temperature is 50~55 DEG C, extracts Time is 0.5~1 hour.
Extracting method the most according to claim 1, it is characterised in that: described eluent is concentrated in vacuo to the 35% of original volume ~45%.
Extracting method the most according to claim 1, it is characterised in that: the eluent after concentration and beta-schardinger dextrin-, Arab The weight ratio of glue, maltodextrin and soybean lecithin is 100:1~3:2~5:0.5~1:0.1~0.3.
CN201610628239.2A 2016-08-03 2016-08-03 The extracting method of Fructus Momordicae polyphenol Pending CN106074669A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610628239.2A CN106074669A (en) 2016-08-03 2016-08-03 The extracting method of Fructus Momordicae polyphenol

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610628239.2A CN106074669A (en) 2016-08-03 2016-08-03 The extracting method of Fructus Momordicae polyphenol

Publications (1)

Publication Number Publication Date
CN106074669A true CN106074669A (en) 2016-11-09

Family

ID=57455088

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610628239.2A Pending CN106074669A (en) 2016-08-03 2016-08-03 The extracting method of Fructus Momordicae polyphenol

Country Status (1)

Country Link
CN (1) CN106074669A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942734A (en) * 2017-03-29 2017-07-14 桂林实力科技有限公司 The extracting method of Momordica grosvenori active ingredient
CN107348367A (en) * 2017-06-27 2017-11-17 广东省农业科学院蚕业与农产品加工研究所 A kind of method that jerky antioxidant effect is improved using mulberry fruit polyphenol extract
CN107362080A (en) * 2017-07-18 2017-11-21 上海应用技术大学 A kind of extracting method of polyphenol in passion fruit pericarp
CN109170678A (en) * 2018-10-15 2019-01-11 贵阳学院 A kind of pre-treating method improving kiwi peer Determination of Polyphenols
CN109674843A (en) * 2019-01-30 2019-04-26 湖南绿蔓生物科技股份有限公司 A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101575326A (en) * 2009-06-25 2009-11-11 合肥工业大学 Method for extracting tea polyphenol from glede tea
CN104886577A (en) * 2015-06-16 2015-09-09 福建省农业科学院农业工程技术研究所 Preparation method of prunus fruit polyphenol

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101575326A (en) * 2009-06-25 2009-11-11 合肥工业大学 Method for extracting tea polyphenol from glede tea
CN104886577A (en) * 2015-06-16 2015-09-09 福建省农业科学院农业工程技术研究所 Preparation method of prunus fruit polyphenol

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘灿 等: "闪式提取法提取罗汉果多酚", 《食品科学》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942734A (en) * 2017-03-29 2017-07-14 桂林实力科技有限公司 The extracting method of Momordica grosvenori active ingredient
CN107348367A (en) * 2017-06-27 2017-11-17 广东省农业科学院蚕业与农产品加工研究所 A kind of method that jerky antioxidant effect is improved using mulberry fruit polyphenol extract
CN107362080A (en) * 2017-07-18 2017-11-21 上海应用技术大学 A kind of extracting method of polyphenol in passion fruit pericarp
CN107362080B (en) * 2017-07-18 2019-10-29 上海应用技术大学 A kind of extracting method of polyphenol in passion fruit pericarp
CN109170678A (en) * 2018-10-15 2019-01-11 贵阳学院 A kind of pre-treating method improving kiwi peer Determination of Polyphenols
CN109674843A (en) * 2019-01-30 2019-04-26 湖南绿蔓生物科技股份有限公司 A kind of method for extraction and purification of dried fructus momordicae comprehensive utilization

Similar Documents

Publication Publication Date Title
CN106074669A (en) The extracting method of Fructus Momordicae polyphenol
CN105767396A (en) Lipid-lowering ginkgo seed tea
CN104098641B (en) A kind of method extracting mogroside V from Grosvenor Momordica
CN103652726A (en) Lotus root starch manufacturing technology based on complete utilization of lotus root
CN103145872B (en) Panax notoginseng polysaccharide with low ash content and preparation method of polysaccharide
CN106805180A (en) A kind of method that polyphenol substance is extracted in desmoenzyme and the ultrasonically treated endotesta walnut kernel by band
CN108794651A (en) The method that pectin is extracted in passion fruit pericarp
CN102823785B (en) Mixed cherokee rose fruit jelly and method for making same
CN107519445A (en) A kind of spacetabs type chronic gastritis Chinese medicinal granule and preparation method thereof
CN105996028A (en) Ginger extract, ginger fiber and preparation method of ginger extract and ginger fiber
CN106070654A (en) A kind of walnut beverage and preparation method thereof
CN106071533A (en) A kind of Radix Puerariae walnut peptide composite beverage and preparation method thereof
CN106343104A (en) Making method of care powder with fructus trichosanthis and dendrobe
CN105875935A (en) Pearl barley effervescent tea tablets capable of eliminating dampness
CN106244333A (en) A kind of preparation method and applications of tobacco aromatics using
CN105685966A (en) Method for extracting flavonoids compounds from silybum marianum seedcake dregs
CN105541957A (en) Extraction method for increasing content of glycyrrhizic acid in leftover materials of liquorice
CN105104934A (en) Sour and sweet purple potato starch added with hawthorns
KR101929664B1 (en) Fermented-white ginseng extracts containing increased ginsenoside Rd, Rg3 and Rg5 and method thereof
CN109170885A (en) A method of extracting ginseng effective component
CN109662236A (en) A kind of food additives of the more saponin(es of selenium-rich polysaccharide, preparation method and applications
CN101972283B (en) Preparation method of common wedgelet fern herb total flavone
CN103830490A (en) Extraction method of selenium-rich Huai rhizoma dioscoreae extract
CN106509556A (en) Eleutherine americana oxidation-resistant beverage and preparation method thereof
CN106260202A (en) A kind of kidney-replenishing blood-nourishing Radix Polygoni Multiflori Paeonia suffruticosa tea and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20161109