CN105953753A - Measuring device and method of porphyra haitanensis area - Google Patents
Measuring device and method of porphyra haitanensis area Download PDFInfo
- Publication number
- CN105953753A CN105953753A CN201610399499.7A CN201610399499A CN105953753A CN 105953753 A CN105953753 A CN 105953753A CN 201610399499 A CN201610399499 A CN 201610399499A CN 105953753 A CN105953753 A CN 105953753A
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- light source
- porphyra haitanensis
- area
- thallus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/28—Measuring arrangements characterised by the use of optical techniques for measuring areas
- G01B11/285—Measuring arrangements characterised by the use of optical techniques for measuring areas using photoelectric detection means
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention belongs to the field of blade measurement and particularly relates to a measuring device of a porphyra haitanensis area. The device comprises an object lens and a sample platform. The object lens is above the sample platform. The bottom end of the object lens is provided with a color filtering lens. The sample platform is equipped with a sample. A main light source is arranged under the sample platform. The object lens, the color filtering lens, the sample, the sample platform and the main light source are arranged in the same straight line. The device is a very stable and reliable selection for measuring the blade area of a porphyra haitanensis and has the characteristics of accurate measurement, good repeatability, and simple operation, the method is simple and easy and has higher accuracy and a better effect compared with a method with the use of a length and a width.
Description
Technical field
The invention belongs to field of vane measurement, be specifically related to measurement apparatus and the method for a kind of porphyra haitanensis thallus area.
Background technology
Leaf is the most important place that higher plant carries out photosynthesis and transpiration.The size of leaf area be embody plant growing,
The important indicator of nutriture, is the key factor determining Biomass and yield.The measurement of leaf area has been answered in higher plant
With, it is the research growth promoter of plant, yield composition and the important character index of Planting characteristic.Leaf is measured many higher plants
In the method for area, such as Publication No. " CN105180851A ", the invention of entitled " pine needle area measuring device and method "
Patent, discloses measurement device pine needle areas such as utilizing optical glass lens, light source, imageing sensor, with default pine needle
Areal calculation model compares acquisition pine needle area to be measured.Such as Publication No. " CN105004284A ", entitled " a kind of photo-electric
Nicotiana tabacum L. area measuring device " patent of invention, disclose use photovoltaic panel principle measure Nicotiana tabacum L. area.Conventional leaf area instrument method
Measure, such as Publication No. " CN105136070A ", sending out of entitled " utilizing the method that plant leaf area measured by electronic equipment "
Bright patent, discloses and is laid in by mounted blade to be measured on the reference plate with canonical reference pattern, and canonical reference pattern has base
Quasi-area；Electronic equipment is utilized to take pictures to obtain the photo of whole reference plate to the reference plate being covered with described blade to be measured；Meter
Calculate the real area size corresponding to each pixel in photo；Pixel quantity occupied by blade to be measured, calculates described blade to be measured
Real area size.Such as Publication No. " CN103791861A ", entitled " a kind of plant leaf area non-destructive determination method "
Patent of invention, discloses and have employed leaf image acquisition method and indoor leaf area meter algorithm measures and calculates, wherein
Leaf image acquisition method uses digital camera, shooting background base plate, graph paper.Such as Publication No. " CN101726267A ", title
For " a kind of living body blade area tester and test leaf area method " patent of invention, disclose include backing plate, cover plate,
Component measured by housing, longitudinal pulse generator, photoelectric sensor, film key and lining rope etc..
Porphyra haitanensis is the ocean cryptogam that China coast is important, and its thallus is made up of cell monolayer, film like, size
Relevant with yield.Compared with the leaf of higher plant, it is irregular equally, but its profile and edge have along with growth and gathering etc.
Significant change.Traditional method is to use the index such as length and width and length and width ratio to measure, but thallus changes greatly, such as,
Seedling Stage length and width ratio is 10-100, at the end of, 1-0.3, only determine with a width of index or to predict that growth has sizable with long
Error.Meanwhile, thickness is thin, and only about 1/100, thallus color differs, and moisture content is high, with the background such as glass, water
Contrast little, obscuring easily occurs with background in thallus border, and therefore, the error of Direct Determination is big.By measuring blade face
Amass reaction yield significant in the Sargassums such as Thallus Porphyrae, the most also do not have and see the research report measuring leaf area.
Summary of the invention
It is an object of the invention to the measurement apparatus by providing a kind of porphyra haitanensis thallus area, and according to actual situation, determine
Suitable measuring method, it is achieved the measurement of porphyra haitanensis thallus leaf area.
A kind of measurement apparatus of porphyra haitanensis thallus area, including object lens and print platform, described object lens are positioned at the top of print platform,
The bottom of described object lens is provided with colour filter, and equipped with print on described print platform, the lower section of described print platform is provided with main light source, described
Object lens, colour filter, print, print platform and main light source all arrange point-blank.
Further, described sample stage be provided around side light source.
Further, described side light source is tabular.
Preferably, described colour filter is faint yellow.
Preferably, described print platform uses transparent quartz glass.
The measuring method of a kind of porphyra haitanensis thallus area, comprises the following steps:
(1) print is prepared: try one's best as print so smooth that to be layered on collection plate using porphyra haitanensis thallus, after paving, use absorbent paper
Suck surface moisture gently, then an acrylic acid overlay film is covered on print surface, by 0.01cm2—1.0cm2Square red
Coloured paper sheet is placed on sample corner as scale, in order to calculate Thallus Porphyrae leaf area afterwards；
(2) regulation main light source and the intensity of illumination of side light source；
(3) shooting: install camera, shoots porphyra haitanensis thallophytic print photo；
(4) data process: changed by figure, stored and edit by the photo that camera shooting obtains, and quickly measure porphyra haitanensis leaf
The leaf area of shape body, the simultaneously further feature such as the thallophytic length of accurate recording porphyra haitanensis, width.
Preferably, in step (1) by acrylic acid overlay film cover when print surface, from the beginning of the side of print, after be extended down to whole
Print, prevents bubble from producing.
Preferably, in step (2), when keeping measuring, the intensity of illumination of main light source is 1000Lux 3000Lux, side every time
The intensity of illumination of light source is 50Lux-300Lux.
Preferably, in step (3), it is fixed to certain altitude with portable rack during shooting photo and shoots, and take to hang down
Pen-hold grip is taken the photograph, and closest requirement of taking pictures is the red scraps of paper of four squares that can just see collection plate summit.
The Advantageous Effects of the present invention: the measurement apparatus of the porphyra haitanensis thallus area of the present invention and method are for measuring porphyra haitanensis
The features such as thallophytic leaf area is a highly stable reliable selection, and it has mensuration accurately, reproducible, simple to operate,
Employing the method is simple and easy to do, uses more merely Length x Width more accurate, effective.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention；
Reference: 1, object lens；2, colour filter；3, print；4, print platform；5, main light source；6, side light source.
Detailed description of the invention
The present invention is further detailed explanation with detailed description of the invention below in conjunction with the accompanying drawings.
A kind of measurement apparatus of porphyra haitanensis thallus area, as it is shown in figure 1, include object lens 1 and print platform 4, described object lens 1
Being positioned at the top of print platform 4, the bottom of described object lens 1 is provided with colour filter 2, it is possible to obtain suitable spectrum, obtains with record
Image clearly, described colour filter 2 is faint yellow；Described print platform 4 uses transparent quartz glass, on described print platform 4
Equipped with print 3, the top of described print 3 is coated with one layer of acrylic acid overlay film, and the lower section of described print platform 4 is provided with main light source 5,
Described object lens 1, colour filter 2, print 3, print platform 4 and main light source 5 all arrange point-blank；Described sample stage 4
Being provided around side light source 6, described side light source 6 is plate-shaped light source, print platform 4 and print 3 carries out three-dimensional encirclement, thus disappears
Error except print 3 with print platform 4.Using artificial lighting during use, the light of main light source (5) first passes through print platform (4),
The image of print (3) passes through colour filter (2), enters the object lens (1) of camera, thus obtains record.
The measuring method of this device comprises the following steps:
(1) print is prepared: porphyra haitanensis thallus is so the most smooth that to be layered on collection plate as print 3, with water suction after paving
Paper sucks surface moisture gently, now, if Thallus Porphyrae is long, can curve it to measure, if being by the experiment of non-succession,
Thallus Porphyrae can be cut into several sections to measure；Again an acrylic acid overlay film is covered on print 3 surface, during operation, from the one of print
Side starts, after be extended down to whole print, prevent bubble from producing；By 0.01cm2—1.0cm2The red scraps of paper of square as ratio
Chi is placed on sample corner, in order to calculating Thallus Porphyrae leaf area afterwards, the principle of calculating is that the size of proportionally chi is weighed, by institute
Thallus area to be measured, is divided into the pixel of several bulks, and pixel is the biggest, and block number is the most, shows that the precision measured is got over
High.Seedling Stage, when thallus width is about 1.0cm, uses 0.01cm2The scraps of paper.
(2) regulation main light source and the intensity of illumination of side light source: measure under conditions of uniform light, abundance, uses regulation
Light source and light intensity, reduce the error of the shade generation that light irradiates, for guaranteeing the accuracy measured, master when should keep measuring every time
The intensity of illumination of light source is 1000Lux 3000Lux, and the intensity of illumination of side light source is 50Lux-300Lux, reduces light and irradiates
Shade produce error.
(3) shooting: install camera, shoots porphyra haitanensis thallophytic print photo, is fixed to necessarily with portable rack during shooting
Highly shooting, and take vertically to shoot, closest requirement of taking pictures is four pros that can just see collection plate summit
The shape redness scraps of paper.
(4) data process: changed by figure, stored and edit by the photo that camera shooting obtains, and quickly measure porphyra haitanensis leaf
The leaf area of shape body, the simultaneously further feature such as the thallophytic length of accurate recording porphyra haitanensis, width.
The highest coefficient of variation of pixel is the least, but 8,000,000 pixels and 10,000,000 pixel difference are not notable (being shown in Table 1), this method
Use and shoot with the camera of 8,000,000 pixels.
Table 1 different pixels shoots the coefficient of variation of leaf area
| 1000000 pixels | 3000000 pixels | 5000000 pixels | 8000000 pixels | 10000000 pixels | |
| Coefficient of variation % | 1.717±0.349a | 0.536±0.173b | 0.215±0.065c | 0.127±0.036d | 0.105±0.037d |
Note: different letter representation significant differences
Measuring Thallus Porphyrae leaf area in the environment of different temperatures and humidity, difference is notable, temperature and wet when showing to measure leaf area
The difference of degree there is no the biggest impact to result.And the measurement of leaf area is affected relatively big by illumination, under different light intensity, measure Thallus Porphyrae
Leaf area, has certain error.Measure under conditions of uniform light, abundance.
Although, the present invention is described in detail to have used general explanation and specific embodiment, but at base of the present invention
On plinth, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, the most inclined
These modifications or improvements on the basis of spirit of the present invention, belong to the scope of protection of present invention.
Claims (9)
1. the measurement apparatus of a porphyra haitanensis thallus area, it is characterized in that: include object lens and print platform, described object lens are positioned at the top of print platform, the bottom of described object lens is provided with colour filter, equipped with print on described print platform, the lower section of described print platform is provided with main light source, and described object lens, colour filter, print, print platform and main light source all arrange point-blank.
The measurement apparatus of porphyra haitanensis thallus area the most according to claim 1, it is characterised in that: described sample stage be provided around side light source.
The measurement apparatus of porphyra haitanensis thallus area the most according to claim 2, it is characterised in that: described side light source is tabular.
The measurement apparatus of porphyra haitanensis thallus area the most according to claim 1, it is characterised in that: described colour filter is faint yellow.
The measurement apparatus of porphyra haitanensis thallus area the most according to claim 1, it is characterised in that: described print platform uses transparent quartz glass.
6. according to the measuring method of the measurement apparatus of the porphyra haitanensis thallus area described in any one of claim 2-5, it is characterised in that comprise the following steps:
(1) prepare print: try one's best as print so smooth that to be layered on collection plate using porphyra haitanensis thallus, after paving, suck surface moisture with absorbent paper gently, then an acrylic acid overlay film is covered on print surface, by 0.01cm2—1.0cm2The red scraps of paper of square be placed on sample corner as scale, in order to calculate Thallus Porphyrae leaf area afterwards；
(2) regulation main light source and the intensity of illumination of side light source；
(3) shooting: install camera, shoots porphyra haitanensis thallophytic print photo；
(4) data process: changed by figure, stored and edit by the photo that camera shooting obtains, and quickly measure the thallophytic leaf area of porphyra haitanensis, simultaneously the further feature such as the thallophytic length of accurate recording porphyra haitanensis, width.
Measuring method the most according to claim 6, it is characterised in that: in step (1) by acrylic acid overlay film cover when print surface, from the beginning of the side of print, after be extended down to whole print, prevent bubble from producing.
Measuring method the most according to claim 6, it is characterised in that: in step (2), when keeping measuring, the intensity of illumination of main light source is 1000Lux 3000Lux, and the intensity of illumination of side light source is 50Lux-300Lux every time.
Measuring method the most according to claim 6, it is characterized in that: in step (3), it is fixed to certain altitude with portable rack during shooting photo shoot, and taking vertically to shoot, closest requirement of taking pictures is the red scraps of paper of four squares that can just see collection plate summit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610399499.7A CN105953753A (en) | 2016-06-06 | 2016-06-06 | Measuring device and method of porphyra haitanensis area |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610399499.7A CN105953753A (en) | 2016-06-06 | 2016-06-06 | Measuring device and method of porphyra haitanensis area |
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| CN105953753A true CN105953753A (en) | 2016-09-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610399499.7A Pending CN105953753A (en) | 2016-06-06 | 2016-06-06 | Measuring device and method of porphyra haitanensis area |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6490066A (en) * | 1987-09-30 | 1989-04-05 | Mita Industrial Co Ltd | Method for removing paint film from end of drum |
| CN101548670A (en) * | 2009-04-21 | 2009-10-07 | 宁波大学 | Method for praparing varek specimen |
| CN101650316A (en) * | 2009-09-15 | 2010-02-17 | 合肥安大电子检测技术有限公司 | Device and method for detecting tobacco strip structure on line |
| CN201413131Y (en) * | 2009-05-22 | 2010-02-24 | 青岛科技大学 | Novel device for measuring and calculating leaf area index |
| CN102519971A (en) * | 2011-12-01 | 2012-06-27 | 浙江工商大学 | On-line identification apparatus and method for impurities in edible fungi |
| CN105180851A (en) * | 2015-09-21 | 2015-12-23 | 北京农业智能装备技术研究中心 | Pine needle leaf area measurement device and method |
-
2016
- 2016-06-06 CN CN201610399499.7A patent/CN105953753A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6490066A (en) * | 1987-09-30 | 1989-04-05 | Mita Industrial Co Ltd | Method for removing paint film from end of drum |
| CN101548670A (en) * | 2009-04-21 | 2009-10-07 | 宁波大学 | Method for praparing varek specimen |
| CN201413131Y (en) * | 2009-05-22 | 2010-02-24 | 青岛科技大学 | Novel device for measuring and calculating leaf area index |
| CN101650316A (en) * | 2009-09-15 | 2010-02-17 | 合肥安大电子检测技术有限公司 | Device and method for detecting tobacco strip structure on line |
| CN102519971A (en) * | 2011-12-01 | 2012-06-27 | 浙江工商大学 | On-line identification apparatus and method for impurities in edible fungi |
| CN105180851A (en) * | 2015-09-21 | 2015-12-23 | 北京农业智能装备技术研究中心 | Pine needle leaf area measurement device and method |
Non-Patent Citations (1)
| Title |
|---|
| 张云鹤: "基于虚拟仪器技术的作物叶片面积测量仪的开发", 《农业工程学报》 * |
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Application publication date: 20160921 |
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