CN105940803B - A kind of method that utilization microbial manure improves orchard soil - Google Patents
A kind of method that utilization microbial manure improves orchard soil Download PDFInfo
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Abstract
本发明提供一种利用微生物肥料改良果园土壤的方法,该方法包括如下步骤:(1)将果园土壤进行翻耕,撒施生物有机肥,施用量为1000‑2000kg/亩;翻耕时,避免将下层土壤翻到表层土壤上;(2)利用旋耕机对园区进行旋耕,将土壤耙细、耙平。本发明能显著改良土壤,降低土壤盐分;降低土传病害、苗木定值存活率达到80%以上、提高作物的产量;本发明的方法简单、易于推广使用。The invention provides a kind of method utilizing microbial fertilizer to improve orchard soil, and the method comprises the following steps: (1) plowing the orchard soil, spreading bio-organic fertilizer, application rate is 1000-2000kg/mu; when plowing, avoid Turn the lower layer of soil onto the top layer of soil; (2) use a rotary tiller to perform rotary tillage on the park, and rake and level the soil. The invention can significantly improve the soil, reduce soil salinity, reduce soil-borne diseases, increase the survival rate of seedlings to more than 80 percent, and increase the yield of crops; the method of the invention is simple and easy to popularize and use.
Description
技术领域technical field
本发明属于土壤改良领域,具体涉及一种利用微生物肥料改良果园土壤的方法。The invention belongs to the field of soil improvement, and in particular relates to a method for improving orchard soil by utilizing microbial fertilizers.
背景技术Background technique
在土壤肥力贫瘠的地区,土壤中的盐分含量普遍过高,使得作物出苗后根系容易腐烂,造成作物产量不高。目前,提高作物产量的方法主要靠施用化学农药,而化学农药的施用则进一步增大了土壤贫瘠化的趋势。In areas with poor soil fertility, the salt content in the soil is generally too high, which makes the root system of crops easy to rot after emergence, resulting in low crop yield. At present, the method of increasing crop yield mainly relies on the application of chemical pesticides, and the application of chemical pesticides further increases the tendency of soil barrenness.
因此,如何利用绿色肥料,特别是微生物肥料对果园土壤进行改良,成为亟待解决的技术问题。Therefore, how to use green fertilizers, especially microbial fertilizers, to improve orchard soil has become a technical problem to be solved urgently.
发明内容Contents of the invention
针对现有技术的缺点,本发明的目的在于提供一种利用微生物肥料改良果园土壤的方法,该方法包括如下步骤:For the shortcoming of prior art, the object of the present invention is to provide a kind of method utilizing microbial fertilizer to improve orchard soil, and this method comprises the steps:
(1)将果园土壤进行翻耕,撒施生物有机肥,施用量为1000-2000kg/亩;翻耕时,避免将下层土壤翻到表层土壤上;(1) Plow the orchard soil, and spread bio-organic fertilizer at an application rate of 1000-2000kg/mu; when plowing, avoid turning the lower layer of soil onto the surface soil;
(2)利用旋耕机对园区进行旋耕,将土壤耙细、耙平;(2) Use the rotary tiller to perform rotary tillage on the park, and rake and level the soil;
所述生物有机肥由方法A或方法B制得:Described biological organic fertilizer is made by method A or method B:
方法A包括步骤:Method A consists of the steps:
发酵原料的制备:调节发酵基底的C/N比为20-25,总养分含量为6.0~6.5%;所述发酵基底包括菌渣;Preparation of fermentation raw materials: adjusting the C/N ratio of the fermentation base to 20-25, and the total nutrient content to 6.0-6.5%; the fermentation base includes fungus residue;
预发酵:设置堆体高度≤3m,发酵原料含水量为50~60%;Pre-fermentation: Set the height of the pile to be ≤3m, and the moisture content of the fermentation raw materials to be 50-60%;
槽式发酵:将步骤所得物进行入槽堆放,设置堆体高度≤3m,发酵原料含水量为40~50%;当堆体温度升高到50~55℃且持续1天以上时进行翻肥,每隔2~4天翻一次肥,槽式发酵的时间为20~25天;Tank-type fermentation: stack the obtained products in the tank, set the height of the pile to ≤3m, and the water content of the fermentation raw materials is 40-50%; when the temperature of the pile rises to 50-55°C and lasts for more than 1 day, turn over the fertilizer , turn over the fertilizer every 2 to 4 days, and the time of tank fermentation is 20 to 25 days;
④堆沤发酵:设置堆体高度≤6m,步骤所得物含水量为35~40%;当堆体温度升高到55℃以上并持续2~3天后,进行翻肥,每隔5~7天翻一次肥,堆沤发酵的时间为20~25天;④Stack retting fermentation: set the height of the pile to ≤6m, and the water content of the product obtained in the step is 35-40%; when the temperature of the pile rises above 55°C and lasts for 2-3 days, turn over the fertilizer, every 5-7 days Turn over the fertilizer once, and the time for composting and fermentation is 20 to 25 days;
⑤破碎分筛:当堆体温度持续保持在50℃及以下,步骤④所得物含水量为35%及以下时,对步骤所得物进行破碎分筛;⑤ Crushing and sieving: When the temperature of the pile remains at 50°C or below and the water content of the product in step ④ is 35% or below, the product in step 4 is crushed and sieved;
⑥后续腐熟发酵:将步骤⑤所得物进行后续腐熟发酵,设置堆体高度≤6m,每7~10天翻一次肥,当堆体温度持续保持在40℃及以下,步骤⑤所得物含水量保持在30%及以下时,有机肥已腐熟完成,即得生物有机肥;⑥ Subsequent decomposing fermentation: Subsequent decomposing and fermenting the product obtained in step ⑤, setting the height of the pile to be ≤ 6m, and turning the fertilizer every 7 to 10 days. When it is 30% or less, the organic fertilizer has been fully decomposed, and the bio-organic fertilizer is obtained;
方法B包括步骤:Method B includes the steps of:
发酵原料的制备:调节发酵基底的C/N比为20-25,总养分含量为6.0~6.5%;所述发酵基底包括菌渣;Preparation of fermentation raw materials: adjusting the C/N ratio of the fermentation base to 20-25, and the total nutrient content to 6.0-6.5%; the fermentation base includes fungus residue;
预发酵:将步骤中所得发酵原料进行条垛堆肥,设置堆体高度≤2m,每间隔1m进行打孔,打孔直径为3-5cm,打孔深到底,当堆体温度升高到50℃以上时,进行一次翻肥,并转入堆沤发酵区;Pre-fermentation: Compost the fermented raw materials obtained in the step, set the height of the pile to ≤ 2m, and punch holes at intervals of 1m. The diameter of the holes is 3-5cm, and the hole is deep to the bottom. When above, carry out a fertilizer turning, and transfer to the compost retting fermentation area;
堆沤发酵:将步骤所得物进行堆沤发酵,设置堆体高度≤3m,当堆体温度升高到55℃以上且持续2-3天时进行翻肥,每隔3~5天翻一次肥,堆沤发酵的时间为20-30天;Compost retting fermentation: carry out compost retting fermentation on the obtained products in the steps, set the height of the pile body to be ≤3m, turn over the fertilizer when the temperature of the pile body rises above 55°C and lasts for 2-3 days, and turn the fertilizer every 3 to 5 days, The time for stack retting fermentation is 20-30 days;
④后续腐熟发酵:将步骤所得物进行后续腐熟发酵,设置堆体高度≤6m,当堆体温度达50℃以上时,进行翻肥,每7~10天翻一次肥,当堆体温度持续保持在40℃及以下,步骤所得物含水量保持在30%及以下时,有机肥已腐熟完成,即得生物有机肥。④ Subsequent decomposing and fermentation: carry out subsequent decomposing and fermenting the obtained products in the step, set the height of the pile to be ≤ 6m, and turn over the fertilizer when the temperature of the pile reaches above 50°C, and turn over the fertilizer every 7 to 10 days. When the water content of the product obtained in the step is kept at 30% or below at 40° C. or below, the organic fertilizer has been fully decomposed, and the bio-organic fertilizer is obtained.
如本发明的实验例所示,本发明能够显著的降低土壤中的盐分含量,并显著的提高作物的成苗率和产量,同时降低作物的患病率。As shown in the experimental examples of the present invention, the present invention can significantly reduce the salinity content in the soil, significantly increase the seedling rate and yield of crops, and simultaneously reduce the disease rate of crops.
优选的,所述方法还包括如下步骤:在步骤(2)之后,进行步骤:于厢面开挖定植穴,每穴深20-40cm,宽40-60cm,向穴中加入3-8kg生物有机肥并与穴中土壤混合,然后将3-8kg生物有机肥撒施在挖出的土壤里,回填至穴中,形成高15-20cm,直径60-80cm的中间高四周低的树盘。更优选的,开挖定植穴后,向穴中加入5kg生物有机肥并与穴中土壤混合,然后将5kg生物有机肥和撒施在挖出的土壤里,回填至穴中。Preferably, the method further comprises the following steps: after step (2), carry out the step of excavating planting holes on the compartment surface, each hole is 20-40cm deep and 40-60cm wide, and 3-8kg of biological organic matter is added to the hole. Fertilize and mix with the soil in the hole, then spread 3-8kg of bio-organic fertilizer in the excavated soil, and backfill it into the hole to form a tree disk with a height of 15-20cm and a diameter of 60-80cm in the middle and low in the periphery. More preferably, after excavating the planting hole, add 5kg of bio-organic fertilizer to the hole and mix it with the soil in the hole, then sprinkle 5kg of bio-organic fertilizer on the excavated soil, and backfill it into the hole.
优选的,所述方法A步骤(2)中,当预发酵过程中堆体温度达35~40℃时,预发酵过程中止。Preferably, in step (2) of the method A, when the temperature of the heap during the pre-fermentation reaches 35-40° C., the pre-fermentation process is terminated.
优选的,所述方法A步骤(1)中,发酵基底中菌渣含量按重量百分比计为60%以上;所述方法B步骤(1)中,发酵基底中菌渣含量按重量百分比计为60%以上。Preferably, in the step (1) of the method A, the content of the slag in the fermentation base is more than 60% by weight; in the step (1) of the method B, the content of the slag in the fermentation base is 60% by weight %above.
优选的,所述方法A步骤(1)中,所述发酵基底还包括城市生活垃圾、农作物秸秆和畜禽粪便中的至少一种;所述方法B步骤(1)中,所述发酵基底还包括城市生活垃圾、农作物秸秆和畜禽粪便中的至少一种。Preferably, in the step (1) of the method A, the fermented substrate further includes at least one of municipal solid waste, crop stalks and livestock and poultry manure; in the step (1) of the method B, the fermented substrate also includes Including at least one of municipal solid waste, crop straw and livestock and poultry manure.
本发明的有益效果:Beneficial effects of the present invention:
1、能显著改良土壤,降低土壤盐分;1. Can significantly improve the soil and reduce soil salinity;
2、降低土传病害、苗木定值存活率达到80%以上、提高作物的产量;2. Reduce soil-borne diseases, the survival rate of seedlings can reach more than 80%, and increase the yield of crops;
3、本发明的方法简单、易于推广使用。3. The method of the present invention is simple and easy to popularize and use.
具体实施方式detailed description
下面通过实施例对本发明进行具体描述,有必要在此指出的是以下实施例只是用于对本发明进行进一步的说明,不能理解为对本发明保护范围的限制,该领域的技术熟练人员根据上述发明内容所做出的一些非本质的改进和调整,仍属于本发明的保护范围。The present invention is described in detail by the following examples, it is necessary to point out that the following examples are only used to further illustrate the present invention, and can not be interpreted as limiting the scope of the present invention, those skilled in the art according to the content of the above invention Some non-essential improvements and adjustments still belong to the protection scope of the present invention.
实施例1Example 1
1)将果园土壤进行翻耕,撒施生物有机肥,施用量为1000kg/亩;翻耕时,避免将下层土壤翻到表层土壤上;1) Plow the orchard soil, and spread bio-organic fertilizer at an application rate of 1000kg/mu; when plowing, avoid turning the lower layer of soil onto the surface soil;
2)利用旋耕机对园区进行旋耕,将土壤耙细、耙平;再按照定植株行距进行开沟,整理厢面。2) Use a rotary tiller to carry out rotary tillage on the park, and rake the soil finely and level; then ditch according to the row spacing of the fixed plants, and tidy up the car surface.
制备生物有机肥的方法为:The method for preparing bio-organic fertilizer is:
(1)发酵原料的制备:按重量份计,取60份菌渣、30份牛粪、10份餐厨垃圾混合均匀,向其中加入枯草芽孢杆菌、地衣芽孢杆菌和氮肥、磷肥、钾肥,调节总混合料的C/N比为22,总养分含量为6.3%;(1) Preparation of fermentation raw material: by weight, get 60 parts of bacteria residues, 30 parts of cow dung, 10 parts of kitchen waste and mix evenly, add Bacillus subtilis, Bacillus licheniformis and nitrogen fertilizer, phosphate fertilizer, potassium fertilizer to it, adjust The C/N ratio of the total mixture is 22, and the total nutrient content is 6.3%;
(2)槽式发酵:将步骤(1)所得发酵原料进行入槽堆放,设置堆体高度为2.5m,发酵原料含水量为50%,每天记录观察30cm深处堆体温度变化情况,当堆体温度升高到52℃且持续2天时使用翻肥机进行翻肥,每隔4天翻一次肥,发酵时间为22天;(2) Tank type fermentation: the fermented material obtained in step (1) is put into the tank and piled up, the height of the heap is set to be 2.5m, and the moisture content of the fermented raw material is 50%. When the body temperature rises to 52°C and continues for 2 days, use a fertilizer turning machine to turn the fertilizer every 4 days, and the fermentation time is 22 days;
(3)堆沤发酵:经22天槽式发酵后,步骤(2)所得物已无明显臭味,此时将其从发酵槽中转出,进行堆沤发酵,堆体高度为5m,步骤(2)所得物含水量为38%;每天记录观察30cm深处堆体温度变化情况,当堆体温度升高到58℃并持续3天后,使用大型装载机进行翻肥,每隔6天翻一次肥,发酵时间为23天;(3) Stack retting fermentation: after 22 days of trough fermentation, the product of step (2) has no obvious odor, and now it is transferred out from the fermentation tank for stack retting fermentation. The height of the pile is 5m. (2) The water content of the resulting product is 38%; record and observe the temperature change of the pile at a depth of 30cm every day, when the temperature of the pile rises to 58°C and continues for 3 days, use a large loader to turn the fertilizer every 6 days One time fertilizer, the fermentation time is 23 days;
(4)破碎分筛:当堆体温度持续保持在48℃,步骤(3)所得物含水量保持在34%时,使用破碎分筛设备将步骤(3)所得物进行破碎分筛;(4) Crushing and sieving: when the temperature of the pile is kept at 48°C and the water content of the product in step (3) is kept at 34%, use the crushing and sieving equipment to crush and sieve the product in step (3);
(5)后续腐熟发酵:将步骤(4)所得物进行后续腐熟发酵,设置堆体高度为5m,每8天翻一次肥,当堆体温度降至35℃,步骤(4)所得物含水量降至28%时,有机肥已腐熟完成,即得生物有机肥。(5) Subsequent decomposing fermentation: The result of step (4) is subjected to subsequent decomposing and fermenting, the height of the pile is set to 5m, and the fertilizer is turned every 8 days. When the temperature of the pile drops to 35°C, the water content of the result of step (4) When it is reduced to 28%, the organic fertilizer has been fully decomposed, and the bio-organic fertilizer is obtained.
实施例2Example 2
1)将果园土壤进行翻耕,撒施生物有机肥,施用量为2000kg/亩;翻耕时,避免将下层土壤翻到表层土壤上;1) Plow the orchard soil and spread bio-organic fertilizer at an application rate of 2000kg/mu; when plowing, avoid turning the lower layer of soil onto the surface soil;
2)利用旋耕机对园区进行旋耕,将土壤耙细、耙平;再按照定植株行距进行开沟,整理厢面;2) Use a rotary tiller to carry out rotary tillage on the park, rake the soil finely and flatten it; then ditch according to the row spacing of the fixed plants, and tidy up the car surface;
3)于厢面开挖定植穴,每穴深20-40cm,宽40-60cm,向穴中加入3-8kg生物有机肥并与穴中土壤混合,然后将3-8kg生物有机肥撒施在挖出的土壤里,回填至穴中,形成高15-20cm,直径60-80cm的中间高四周低的树盘。3) Excavate planting holes on the surface of the car, each hole is 20-40cm deep and 40-60cm wide, add 3-8kg bio-organic fertilizer to the hole and mix with the soil in the hole, and then spread 3-8kg bio-organic fertilizer on the In the excavated soil, backfill in the hole to form a tree disk with a height of 15-20cm and a diameter of 60-80cm, which is high in the middle and low around.
制备生物有机肥的方法为:The method for preparing bio-organic fertilizer is:
(1)发酵原料的制备:按重量份计,取70份菌渣、10份猪粪、20份牛粪混合均匀,向其中加入枯草芽孢杆菌、侧孢短芽孢杆菌和氮肥、磷肥、钾肥,调节总混合料的C/N比为25,总养分含量为6.5%;(1) Preparation of fermentation raw material: by weight, get 70 parts of bacteria residues, 10 parts of pig manure, 20 parts of cow manure and mix uniformly, add Bacillus subtilis, Brevibacillus serospora and nitrogen fertilizer, phosphorus fertilizer, potash fertilizer wherein, Adjust the C/N ratio of the total mixture to be 25, and the total nutrient content to be 6.5%;
(2)预发酵:将步骤(1)所得发酵原料进行条垛堆肥,设置堆体高度为1.8m,每间隔1m进行打孔,打孔直径为5cm,打孔深到底,当堆体温度升高到53℃时,进行一次翻肥,并转入堆沤发酵区;(2) Pre-fermentation: Compost the fermented raw materials obtained in step (1) in strips, set the height of the pile to 1.8m, punch holes every 1m, the diameter of the holes is 5cm, and the depth of the holes is 5cm. When the temperature of the pile increases When the temperature reaches 53°C, turn over the fertilizer once and transfer to the composting fermentation area;
(3)堆沤发酵:将步骤(2)所得物进行堆沤发酵,设置堆体高度为2m,每天测定距顶部30cm深处堆体温度,当堆体温度升高到58℃且持续2天时进行翻肥,每隔5天翻一次肥,堆沤发酵的时间为30天;(3) Stack retting fermentation: carry out stack retting fermentation on the product obtained in step (2), set the height of the pile to 2m, measure the temperature of the pile at a depth of 30cm from the top every day, when the temperature of the pile rises to 58°C and lasts for 2 days Fertilize, turn once every 5 days, and the time for composting and fermenting is 30 days;
(4)后续腐熟发酵:经过30天堆沤发酵后,将步骤(3)所得物进行后续腐熟发酵,设置堆体高度为5.5m,每天测定距顶部30cm深处堆体温度,当堆体温度达53℃时,进行翻肥,每10天翻一次肥,当堆体温度降至32℃,步骤(3)所得物含水量降至25%时,有机肥已腐熟完成,即得生物有机肥。(4) Subsequent decomposing fermentation: After 30 days of composting and fermentation, the result of step (3) is subjected to subsequent decomposing and fermenting, the height of the compost is set to 5.5m, and the temperature of the compost at a depth of 30cm from the top is measured every day. When it reaches 53°C, turn the fertilizer, and turn the fertilizer once every 10 days. When the temperature of the heap body drops to 32°C and the water content of the product in step (3) drops to 25%, the organic fertilizer has completely decomposed, and the bio-organic fertilizer is obtained. .
实施例3Example 3
1)将果园土壤进行翻耕,撒施生物有机肥,施用量为1500kg/亩;翻耕时,避免将下层土壤翻到表层土壤上;1) Plow the orchard soil and spread bio-organic fertilizer at an application rate of 1500kg/mu; when plowing, avoid turning the lower layer of soil onto the surface soil;
2)利用旋耕机对园区进行旋耕,将土壤耙细、耙平;再按照定植株行距进行开沟,整理厢面;2) Use a rotary tiller to carry out rotary tillage on the park, rake the soil finely and flatten it; then ditch according to the row spacing of the fixed plants, and tidy up the car surface;
3)于厢面开挖定植穴,每穴深20-40cm,宽40-60cm,向穴中加入5kg生物有机肥并与穴中土壤混合,然后将5kg生物有机肥撒施在挖出的土壤里,回填至穴中,形成高15-20cm,直径60-80cm的中间高四周低的树盘。3) Excavate planting holes on the side of the car, each hole is 20-40cm deep and 40-60cm wide, add 5kg of bio-organic fertilizer to the hole and mix it with the soil in the hole, and then spread 5kg of bio-organic fertilizer on the excavated soil Backfill in the hole to form a tree disk with a height of 15-20cm and a diameter of 60-80cm, which is high in the middle and low around.
制备生物有机肥的方法为:The method for preparing bio-organic fertilizer is:
(1)发酵原料的制备:按重量份计,取60份菌渣、30份牛粪、10份餐厨垃圾混合均匀,向其中加入枯草芽孢杆菌、地衣芽孢杆菌和氮肥、磷肥、钾肥,调节总混合料的C/N比为22,总养分含量为6.3%;(1) Preparation of fermentation raw material: by weight, get 60 parts of bacteria residues, 30 parts of cow dung, 10 parts of kitchen waste and mix evenly, add Bacillus subtilis, Bacillus licheniformis and nitrogen fertilizer, phosphate fertilizer, potassium fertilizer to it, adjust The C/N ratio of the total mixture is 22, and the total nutrient content is 6.3%;
(2)预发酵:将步骤(1)所得发酵原料进行条垛堆肥,设置堆体高度为1.5m,每间隔1m进行打孔,打孔直径为4cm,打孔深到底,当堆体温度升高到52℃时,进行一次翻肥,并转入堆沤发酵区;(2) Pre-fermentation: Compost the fermented raw materials obtained in step (1) in strips, set the height of the pile to 1.5m, punch holes every 1m, the diameter of the holes is 4cm, and the depth of the holes is 4cm. When the temperature of the pile increases When the temperature reaches 52°C, turn over the fertilizer once and transfer to the composting fermentation area;
(3)堆沤发酵:将步骤(2)所得物进行堆沤发酵,设置堆体高度为2.5m,每天测定距顶部30cm深处堆体温度,当堆体温度升高到56℃且持续3天时进行翻肥,每隔4天翻一次肥,堆沤发酵的时间为25天;(3) Stack retting fermentation: carry out stack retting fermentation on the result of step (2), set the height of the pile to 2.5m, measure the temperature of the pile at a depth of 30cm from the top every day, when the temperature of the pile rises to 56°C and last for 3 Turn over the fertilizer every 4 days, and the time for composting and fermenting is 25 days;
(4)破碎分筛:当堆体温度持续保持在48℃,步骤(3)所得物含水量为32%时,对步骤(3)所得物进行破碎分筛;(4) Broken and sieved: when the temperature of the heap remains at 48°C and the water content of the product in step (3) is 32%, the product in step (3) is crushed and sieved;
(5)后续腐熟发酵:将步骤(4)所得物进行后续腐熟发酵,设置堆体高度为5m,每天测定距顶部30cm深处堆体温度,当堆体温度达52℃时,进行翻肥,每8天翻一次肥,当堆体温度降至35℃,步骤(3)所得物含水量降至28%时,有机肥已腐熟完成,即得生物有机肥。(5) Subsequent decomposing and fermenting: carry out subsequent decomposing and fermenting the result of step (4), set the height of the heap to 5m, measure the temperature of the heap at a depth of 30cm from the top every day, and turn over the fertilizer when the temperature of the heap reaches 52°C. Turn over the fertilizer every 8 days. When the temperature of the heap body drops to 35° C. and the water content of the product in step (3) drops to 28%, the organic fertilizer has been fully decomposed, and the bio-organic fertilizer is obtained.
实验例1Experimental example 1
实施实施例1-3所述方法后,检测土壤盐分含量(土壤深度为60-80cm),以未经处理土壤作为对照,结果如表1所示。After implementing the method described in Examples 1-3, the soil salinity content was detected (soil depth is 60-80 cm), and the untreated soil was used as a contrast, and the results are shown in Table 1.
表1Table 1
如表1所示,本发明方法对于土壤脱盐效果好。As shown in Table 1, the method of the present invention has a good effect on soil desalination.
实验例2Experimental example 2
实施例1-3所得生物有机肥的相关指标测定结果见表2。See Table 2 for the relevant index measurement results of the bio-organic fertilizer obtained in Examples 1-3.
表2Table 2
实验例3Experimental example 3
利用实施例1-3记载的方法对土壤进行改良后,进行夏橙的种植,记录苗木存活率和产量,以未经处理的土壤种植夏橙作为对照组1。结果如表3所示。After the soil was improved by the method described in Examples 1-3, summer oranges were planted, seedling survival rate and yield were recorded, and summer oranges were planted in untreated soil as control group 1. The results are shown in Table 3.
表3table 3
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