CN105900934A - 木瓜蛋白酶在卤虫卵去壳方面的应用 - Google Patents
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- A—HUMAN NECESSITIES
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Abstract
本发明公开了一种木瓜蛋白酶在卤虫卵去壳方面的应用,方法为将浸泡后的卤虫卵置于0.01%‑0.5%的木瓜蛋白酶溶液中,室温下搅拌2‑6小时,卵颜色由褐色变为橙红色时将卵与溶液分离,用海水冲洗后即得去壳卵。本发明属于生物法去壳,不引入化学原料,对去壳卵卵膜损伤小,对后续孵化或直接投喂无不良影响,且操作简单。
Description
技术领域
本发明属于卤虫卵应用技术领域,具体涉及木瓜蛋白酶在卤虫卵去壳方面的应用。
背景技术
卤虫又叫丰年虫,是世界性分布的小型甲壳类,生活于盐湖、盐场等高盐水域中。卤虫卵是优质的水产育苗饵料,在水产养殖业具有重要作用。卤虫卵壳是含铁脂蛋白,鱼虾幼苗无法消化,因此一般需要先孵化出卤虫幼体,然后再将幼体作为饵料投喂。孵化后的卵壳和幼体不易彻底分离,易造成部分已孵化幼体随卵壳倒掉而损耗或卵壳和幼体一起被采食导致鱼虾幼苗肠梗阻死亡。卤虫卵去壳后可以直接投喂,也可以孵化出幼体。由于没有卵壳,可以减少幼体破壳时的能量消耗,有利于保留更多营养。在鱼虾育苗中,卤虫卵的费用往往占育苗场50%-70%成本。通过去壳的方法,可以提高孵化率并使卤虫幼体保留更多营养,同时还可以利用大量孵化率低的低质卵,降低育苗成本,对育苗场意义重大。
目前卤虫卵去壳多采用化学法,即用次氯酸钠及氢氧化钠溶液溶解卵壳,去壳后反应无法立即终止,需要将去壳卵捞出清洗除去余氯,此过程中溶液的余氯继续对脱壳卵作用,损伤较大;同时在去壳过程中溶液温度容易升高,影响孵化率,需要采取降温措施;操作过程对孵化率影响很大,工艺不稳定;且卵表面余氯难以彻底去除,投喂时有氯特有气味影响水产幼苗采食,往往还需要添加诱食剂。
木瓜蛋白酶是一种低特异性蛋白水解酶,属于巯基蛋白酶,它具有酶活高、热稳定性好、天然卫生安全等特点。广泛地存在于番木瓜的根、茎、叶和果实内,其中在未成熟的乳汁中含量最丰富。木瓜蛋白酶的活性中心含半胱氨酸,可水解蛋白质和多肽中精氨酸和赖氨酸的羧基端,并能优先水解那些在肽键的N-端具有二个羧基的氨基酸或芳香L-氨基酸的肽键,因此在食品、医药、饲料、日化、皮革及纺织等行业得到广泛应用。
发明内容
本发明的目的在于提供一种菠萝蛋白酶在卤虫卵去壳方面的应用,解决化学法去壳存在的问题,改善去壳卵质量。
1.将商品卤虫卵用淡水浸泡2-4小时,充气搅拌,使卵充分吸水膨胀;
2.分离出卤虫卵,将其置于10倍重量的0.01%-0.5%木瓜蛋白酶溶液中,室温下120r/min搅拌;
3.去壳时间2-6小时,观察卵的颜色由褐色逐渐变为橙红色,将卵与酶溶液分离;
4.将去壳卵置于50倍以上重量海水中,将充气头置于水底,控制进气量使全部水体翻滚,充气搅拌冲洗10分钟,静置,待分层后分离去壳卵,即可用于孵化或直接投喂。
上述过程中所用淡水如果为自来水,需提前进行除氯,减轻对卵的损伤。
所述步骤2中木瓜蛋白酶溶液浓度进一步优选为0.08%-0.2%。
所述步骤3中去壳时间进一步优选为2-3小时。
与其他蛋白酶相比,去壳卵卵膜对木瓜蛋白酶的抗性强,因此可不使用专门的抑制剂终止反应。
与现有技术相比,本发明的有益效果:
本发明属于生物法去壳,所用原料为天然产物,不引入化学原料,对去壳卵无化学污染且对去壳卵卵膜损伤小,对后续孵化或直接投喂无不良影响,降低了操作难度,而且去壳效果稳定。
具体实施方式
下面通过实施例对本发明作进一步详细描述,但是本发明并不限于下述实施方式。
实施例1:
1.将500克卤虫卵用淡水浸泡3小时,充气搅拌,使卵充分吸水膨胀。如果所用淡水为自来水,需提前进行除氯,减轻对去壳卵的损伤;
2.分离出卤虫卵,将其置于5升0.12%的木瓜蛋白酶溶液中,酶活力100万U/g,室温下120r/min搅拌;
3.去壳时间2小时,当卵的颜色由褐色逐渐变为橙红色,将卵与酶溶液分离;
4.将去壳卵重新置于30升海水中,将充气头置于水底,控制进气量使全部水体翻滚,充气搅拌冲洗10分钟,静置,待分层后分离去壳卵,即可用于孵化或直接投喂。
实施例2:不同去壳方法对卤虫卵孵化的影响
采用产地为山东的同批次卤虫卵,在自然光照射、盐度30‰、温度28℃,孵化时间24h,以及其他条件一致的情况下,取两种方法在不同处理时间对应的去壳率及相应去壳卵的孵化率,来对比两种不同的去壳方法对卤虫卵孵化的影响,结果如表1所示。其中:对照组为正常孵化,不去壳;化学法为次氯酸钠和氢氧化钠联用;两种去壳方法的孵化率均为去壳卵孵化率。
表1 不同处理方法对卤虫卵孵化的影响
使用化学法去壳过程中,去壳卵的孵化率与去壳率成负相关,即去壳时间越长则去壳率越高,孵化率越低。虽然去壳率低时的去壳卵孵化率比对照组高,但此时还有相当部分的卵没有去壳,所以卵的总体孵化率提高较小;当完全去壳时,总体孵化率反而比正常孵化低。采用本发明方法去壳,作用温和,对卵损伤小,在去壳率高时的去壳卵孵化率依然较高,使卵的总体孵化率有较大提高,且直接投喂或用于孵化均适宜。
Claims (1)
1.木瓜蛋白酶在卤虫卵去壳方面的应用,其特征在于:将浸泡后的卤虫卵置于0.01%-0.5%的木瓜蛋白酶溶液中,室温下搅拌2-6小时,卵颜色由褐色变为橙红色时将卵与溶液分离,用海水冲洗后即得去壳卵。
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106577408A (zh) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | 一种用于提高孵化率的脱壳卤虫卵的制备方法 |
CN106689063A (zh) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | 一种稻螟赤眼蜂的人工繁育生产方法 |
CN116286749A (zh) * | 2023-03-15 | 2023-06-23 | 广东海洋大学 | 一种溶解剂及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU1124899A1 (ru) * | 1982-06-18 | 1984-11-23 | Сибирский Научно-Исследовательский И Проектно-Конструкторский Институт Рыбного Хозяйства "Лоцман" | Способ подготовки к инкубации иц ракообразных |
US20040121056A1 (en) * | 2002-12-24 | 2004-06-24 | Inve Technologies N.V., A Corporation Of Belgium | Process for decapsulating crustacean or rotifer eggs |
CN102630639A (zh) * | 2012-01-10 | 2012-08-15 | 李新书 | 一种卤虫卵脱壳方法 |
CN104273100A (zh) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | 一种用于提高孵化率的卤虫卵脱壳的方法 |
-
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- 2016-05-13 CN CN201610314078.XA patent/CN105900934B/zh not_active Expired - Fee Related
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU1124899A1 (ru) * | 1982-06-18 | 1984-11-23 | Сибирский Научно-Исследовательский И Проектно-Конструкторский Институт Рыбного Хозяйства "Лоцман" | Способ подготовки к инкубации иц ракообразных |
US20040121056A1 (en) * | 2002-12-24 | 2004-06-24 | Inve Technologies N.V., A Corporation Of Belgium | Process for decapsulating crustacean or rotifer eggs |
CN102630639A (zh) * | 2012-01-10 | 2012-08-15 | 李新书 | 一种卤虫卵脱壳方法 |
CN104273100A (zh) * | 2014-10-21 | 2015-01-14 | 天津海友佳音生物科技股份有限公司 | 一种用于提高孵化率的卤虫卵脱壳的方法 |
Non-Patent Citations (3)
Title |
---|
DONALDE.CAMPTON 等: "卤虫卵去壳和孵化的简单工艺", 《水产科学》 * |
于秀玲 等: "卤虫去壳卵在河蟹育苗中应用的研究", 《水产养殖》 * |
廖永岩 等: "去壳时间和盐度对卤虫去壳卵孵化的影响", 《科学养鱼》 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106577408A (zh) * | 2016-11-22 | 2017-04-26 | 汪逸凡 | 一种用于提高孵化率的脱壳卤虫卵的制备方法 |
CN106577408B (zh) * | 2016-11-22 | 2019-09-03 | 江苏好润生物科技有限公司 | 一种用于提高孵化率的脱壳卤虫卵的制备方法 |
CN106689063A (zh) * | 2016-12-07 | 2017-05-24 | 中国科学院动物研究所 | 一种稻螟赤眼蜂的人工繁育生产方法 |
CN116286749A (zh) * | 2023-03-15 | 2023-06-23 | 广东海洋大学 | 一种溶解剂及其应用 |
CN116286749B (zh) * | 2023-03-15 | 2024-03-26 | 广东海洋大学 | 一种溶解剂及其应用 |
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