CN105875395A - Method for constructing green alga phase in saline-alkali soil underground water shrimp industrial culture pond - Google Patents
Method for constructing green alga phase in saline-alkali soil underground water shrimp industrial culture pond Download PDFInfo
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- CN105875395A CN105875395A CN201610236290.9A CN201610236290A CN105875395A CN 105875395 A CN105875395 A CN 105875395A CN 201610236290 A CN201610236290 A CN 201610236290A CN 105875395 A CN105875395 A CN 105875395A
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- algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
Abstract
The invention provides a method for constructing green alga phase in a saline-alkali soil underground water shrimp industrial culture pond, and belongs to the technical field of aquaculture. The green alga is platymonas helgolandica. The method comprises the following specific steps of: constructing an alga phase during a preparation period, maintaining the alga phase at the early stage of culture and maintaining the alga phase at the middle-late stage of culture. According to the method, euryhalic, high-temperature-resistant and high-adaptability platymonas helgolandica is adopted to construct the alga phase, so that the water quality of brackish water shrimp industrial culture is optimized, and healthy and rapid growth of shrimps is promoted.
Description
Technical field
The invention belongs to aquaculture field, more particularly to a kind of Underground Water in Salinization Area prawn industrial cultivation
The construction method of pond chlorella algae phase.
Background technology
The Litopenaeus vannamei Acute Hepatic pancreatic necrosis syndrome (Acute that vibrio parahaemolytious causes
Hepatopancreas Necrosis Syndrome, AHPNS)/Deaths syndrome (Early Mortality
Syndrome, EMS) etc. disease bring about great losses to shrimp culture industry.As a kind of halophilic bacteria, salinity is more
Low, vibrio parahaemolytious growth and breeding speed is the slowest.Therefore, Underground Water in Salinization Area cultivation Litopenaeus vannamei has relatively
High success rate.Underground Water in Salinization Area due to pH and carbonate alkalinity is higher and water quality is thin, plankton number
Measuring less, therefore experienced shrimp agriculture is often in cistern first " solarization water ", recycling " clear water " supports shrimp.So
And, in the water body that cistern tans by the sun, microalgae multiformity is complicated, and kind is the most unstable, is easily mixed into cyanophyceae etc. and is harmful to
Algae, during causing prawn culturing, microalgae sociales are constantly become feeble and die and succession, it is difficult to formation is stablized excellent
Algae phase.Microcystic aeruginosa, protokaryon unarmored dinoflagellate is often resulted in when particularly cultivating the mistake eutrophication of middle and late stage water body
Etc. harmful microalgae amount reproduction, cause prawn sick or dead.It addition, in breeding process, high density microalgae
Yi Yin precipitation and cause extinction to be obstructed and dead, generation " algae " phenomenon, ultimately result in the mistake of prawn culturing
Lose.
Build stable chlorella algae and can improve prawn culturing success rate mutually.Platymonas helgolandica var is to salinity and temperature
All there is adaptability widely, be chlorella common in prawn culturing water body.Platymonas helgolandica var contains abundant
Unsaturated fatty acid, is bait micro-algae conventional during prawn seed-rearing.There are some researches show, the secretion of flat algae
Thing and fat-soluble extractives etc. have inhibitory action to vibrio and pseudomonas.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Underground Water in Salinization Area prawn industrial cultivation pond green
The construction method of algae algae phase.The method is by choosing eurysalinity, high temperature resistant, and adaptable Qingdao is the most flat
Algae builds stablizes algae phase, thus optimizes brackish water prawn industrial cultivation Water quality, promotes that prawn is healthy fast
Fast-growing is long；
The present invention is achieved by the following technical solutions:
The construction method of a kind of Underground Water in Salinization Area prawn industrial cultivation pond chlorella algae phase, described chlorella is
Platymonas helgolandica var, the algae concretely comprising the following steps the preparatory stage builds mutually, cultivate during early stage algae maintains mutually and cultivates after
Phase algae maintains mutually；
The algae of described preparatory stage builds mutually, including water inlet in culturing pool, after abundant aeration is without chlorine residue, and will
Platymonas helgolandica var algae solution after amplification culture moves into culturing pool and inoculates, initial inoculation concentration be (1-3) ×
104cell/ml.After inoculation, it is ensured that chlorella is in suspended state, observe water body color, optical microscope every day
Lower counting also observes chlorella growth situation.After 3-5 days, in water body, chlorella cell number reaches (0.8-1.0)
×105Cell/ml, can throw in shrimp Seedling；
Described cultivation early stage algae maintains mutually, and cultivation early stage does not change water, blowdown after bait throwing in 1-2h, blowdown every day
Amount is about the 3-5% of total breeding water body, fills new water after blowdown；
Described cultivation middle and late stage algae maintains mutually, cultivates middle and late stage, makes water body chlorella density maintain (0.8-1.0)
×105Cell/ml, supplements and applies an one-lime phosphate for every 10-15 days, and consumption is 3-5g/m3；Every 15-20 days,
Applying a ferric citrate, consumption is 0.2-0.5g/m3；Every 10-15 days, splash the most activated after hay
Bacillus cereus.Cultivation middle and late stage, along with the increase of prawn daily ration, feeding quantity, every day, blowdown flow rate was total breeding water body
5-10%；
Further, before described cultivation early stage is the shrimp long 3cm of Seedling body；
Further, when inoculating Platymonas helgolandica var, Platymonas helgolandica var cell density (5-10) in inoculation liquid ×
105cell/mL；
Further, described culturing pool is provided with microalgae levitation device, and it includes that being positioned at the micropore at the bottom of culturing pool increases
Oxygen pipe, gas stone, discharge and turn to elbow, micro-pore oxygenation pipe parallel laid at the bottom of pond, at the bottom of culturing pool, is supported
Growing pond bottom perimeter to be provided with 2-4 and be fixed on the gas stone at the bottom of culturing pool, directly over gas stone, 1-2 centimeters is perpendicular to support
Growing and be provided with a discharge at the bottom of pond, discharge diameter is more than gas stone diameter, and discharge is fixed on pond by fixed mount
Wall, turns to elbow one end to connect discharge top end opening, and the other end is parallel and is less than water surface 1-3cm, belongs to and opening wide
Formula, turns to elbow consistent with water inlet pipe direction, and gas stone is connected with aerator by trachea with micro-pore oxygenation pipe.
Present invention beneficial effect compared with prior art:
1) choosing eurysalinity, high temperature resistant, adaptable Platymonas helgolandica var builds algae phase, it is ensured that its
The adaptability in brackish water aquaculture pond.
2) by building shrimp culture pond microalgae levitation device, it is ensured that in breeding process, Platymonas helgolandica var begins
It is in suspended state eventually, effectively prevents Platymonas helgolandica var from algae occurring down because of coagulation.
3) cultivation middle and late stage, splash activated after bacillus subtilis, both contributed to ammonia nitrogen, nitrous acid
The removal of the harmful substances such as salt nitrogen, can be again the CO required for Platymonas helgolandica var offer growth2。
4) by controlling microalgae quantity, effectively build and stablize excellent algae phase, beneficially regulating and controlling water quality and prawn
Mushroom out.
Accompanying drawing explanation
Fig. 1 culturing pool and microalgae levitation device profile: 1, culturing pool, 2, micro-pore oxygenation pipe, 3, gas
Stone, 4, discharge, 5, turn to elbow, 6, fixed mount, 7, trachea, 8, aerator.
Detailed description of the invention
Combine accompanying drawing below by specific embodiment and the technology of the present invention is further described explanation.
If no special instructions, the raw material employed in embodiments of the invention is the raw material that this area is common,
Method employed in embodiment, is the conventional method of this area.
The construction method of a kind of Underground Water in Salinization Area prawn industrial cultivation pond chlorella algae phase, described chlorella is
Platymonas helgolandica var, the algae concretely comprising the following steps the preparatory stage builds mutually, cultivate early stage algae maintains mutually, cultivate in after
Phase algae maintains mutually；
The algae of described preparatory stage builds mutually, water inlet in culturing pool, and water filters through 230 eye mesh screens, uses
(2-10)×10-5Bleaching powder processes, and neutralizes with sodium thiosulfate, open micro-pore oxygenation at the bottom of culturing pool after (12-24) h
Pipe, abundant aeration.After starch potassium iodide paper detection is without chlorine residue, can inoculate.During inoculation, will be through expanding
After big cultivation, algae cell density reaches (5-10) × 105The Platymonas helgolandica var algae solution of cell/mL the most uniformly moves into and supports
Growing pond, initial inoculation concentration is (1-3) × 104cell/ml.After inoculation, for guaranteeing that chlorella is in suspended state,
Open the gentle stone of micro-pore oxygenation pipe at the bottom of culturing pool, make cultivation pool inner water form self-loopa.Every day observes water body
Color, counts under optical microscope and observes chlorella growth situation.After 3-5 days, in water body, chlorella cell number reaches
To (0.8-1.0) × 105Cell/ml, can throw in shrimp Seedling.
Described cultivation early stage algae maintains mutually, and cultivation early stage does not change water, blowdown after bait throwing in 1-2h, and blowdown flow rate is about
The 3-5% of total breeding water body, fills new water after blowdown, water filters through 230 eye mesh screens；
Described cultivation middle and late stage algae maintains mutually, cultivates middle and late stage, observes water body color, optical microphotograph every day
Count under mirror and observe Platymonas helgolandica var growing state.According to counted under microscope result, add Qingdao the most flat
Algae culturing liquid or by draining discharge part Platymonas helgolandica var, make micro algae density maintain (0.8-1.0)
×105cell/ml.Within every 10-15 days, supplementing and apply an one-lime phosphate, consumption is 3-5g/m3；Every 15-20 days,
Applying a ferric citrate, consumption is 0.2-0.5g/m3；Cultivation middle and late stage, every 10-15 days, once warp of splashing
Bacillus subtilis after activation.Along with the increase of prawn daily ration, feeding quantity, every day, blowdown flow rate was total breeding water body
5-10%；
Culturing pool described in the present embodiment is provided with microalgae levitation device, as it is shown in figure 1, it includes micro-pore oxygenation
Pipe 2, gas stone 3, discharge 4 and turn to elbow 5, micro-pore oxygenation pipe parallel laid in culturing pool 1 end,
Culturing pool bottom perimeter is provided with 4 gas stones being fixed at the bottom of culturing pool, and directly over gas stone, 1 centimeters vertically cultivates
Being provided with a discharge at the bottom of pond, discharge diameter is more than gas stone diameter, and discharge is fixed on pond by fixed mount 6
Wall, discharge top is connected with turning to elbow 5, turns to elbow one end to connect discharge top end opening, another
Hold row level with both hands and less than water surface 1cm, belong to open type, turn to elbow to be less than the water surface 9 2cm, turn to elbow and water inlet
Pipe direction is consistent, and gas stone is connected with aerator 8 by trachea 7 with micro-pore oxygenation pipe.
Embodiment 1
Experiment carry out at brackish water prawn industrial cultivation test site, Jiangzhou (the experimental period 5-8 month in 2015,
Amount to 84 days).Litopenaeus vannamei breeding density is 300 tails/m3.Experimental water mud sump is circular (radius
=0.97m, highly=1.0m), totally 36, wherein 30 inoculation Platymonas helgolandica vars, operate as stated above；
6 is tradition " solarization water " cultural method, does not inoculate microalgae, and every day, quantity of exchanged water was 10-30%.During experiment,
Salinity (20.78 ± 0.06) ‰, temperature (29.87 ± 0.13) DEG C.
Result shows, during inoculation Platymonas helgolandica var group cultivation, algae is stable mutually, and the tradition cultivation group cultivation later stage
Microcystis aeruginosa and silkweed occur；In inoculation Platymonas helgolandica var group water body, Total inorganic nitrogrn is respectively than tradition cultivation group
Reduce by 89.4% and 90.5%.Inoculation Platymonas helgolandica var group prawn opisthosoma weighs 18.04 ± 1.36g, and cultured prawn produces
Amount 4.41 ± 0.14kg/m3；Tradition cultivation group prawn opisthosoma weighs 14.30 ± 0.08g, cultured prawn yield 2.41 ± 0.04
kg/m3。
Embodiment 2
Experiment carry out at brackish water prawn industrial cultivation test site, Jiangzhou (the experimental period 8-11 month in 2015,
Amount to 105 days).Litopenaeus vannamei breeding density is 400 tails/m3.Experimental water mud sump is square (5.7m × 5.7
M × 1.2m), totally 80, wherein inoculate Platymonas helgolandica var according to the method described above for 70, other 10 do not connect
Plant microalgae.During experiment, salinity (20.15 ± 0.04) ‰, temperature (24.28 ± 2.23) DEG C.
At the end of experiment, in experimental group water body, Total inorganic nitrogrn reduces by 81.2% than matched group, inoculates Platymonas helgolandica var
Group prawn yield (4.1-4.7) kg/m2, and do not inoculate chlorella vulgaris prawn yield only (3.3-3.7) kg/m2。
Claims (4)
1. the construction method of a Underground Water in Salinization Area prawn industrial cultivation pond chlorella algae phase, it is characterised in that described
Chlorella be Platymonas helgolandica var, the algae concretely comprising the following steps the preparatory stage builds mutually, cultivate early stage algae maintain mutually and
Middle and late stage algae maintains mutually in cultivation；
The algae of described preparatory stage builds mutually, including water inlet in culturing pool, after abundant aeration is without chlorine residue, and will
Platymonas helgolandica var algae solution after amplification culture is poured culturing pool into and is inoculated, initial inoculation concentration be (1-3) ×
104Cell/ml, after inoculation, it is ensured that chlorella is in suspended state, observes water body color, optical microscope every day
Lower counting also observes chlorella growth situation, and after 3-5 days, in water body, chlorella cell number reaches (0.8-1.0)
×105Cell/ml, can throw in shrimp Seedling；
Described cultivation early stage algae maintains mutually, and cultivation early stage does not change water, blowdown after bait throwing in 1-2h, and blowdown flow rate is about
For the 3-5% of total breeding water body, after blowdown, fill new water；
Described cultivation middle and late stage algae maintains mutually, cultivates middle and late stage, makes water body chlorella density maintain (0.8-1.0)
×105Cell/ml, supplements and applies an one-lime phosphate for every 10-15 days, and consumption is 3-5g/m3；Every 15-20 days,
Applying a ferric citrate, consumption is 0.2-0.5g/m3, every 10-15 days, splash the most activated after hay
Bacillus cereus, cultivates middle and late stage, and along with the increase of prawn daily ration, feeding quantity, every day, blowdown flow rate was total breeding water body
5-10%.
The structure of a kind of Underground Water in Salinization Area prawn industrial cultivation pond the most according to claim 1 chlorella algae phase
Method, it is characterised in that before described cultivation early stage is the shrimp long 3cm of Seedling body.
The structure of a kind of Underground Water in Salinization Area prawn industrial cultivation pond the most according to claim 1 chlorella algae phase
Method, it is characterised in that when inoculating Platymonas helgolandica var, Platymonas helgolandica var cell density (5-10) in inoculation liquid ×
105cell/mL。
The structure of a kind of Underground Water in Salinization Area prawn industrial cultivation pond the most according to claim 1 chlorella algae phase
Method, it is characterised in that culturing pool is provided with microalgae levitation device, described microalgae levitation device includes being positioned at cultivation
Micro-pore oxygenation pipe, gas stone, discharge at the bottom of pond and turn to elbow, micro-pore oxygenation pipe parallel laid at the bottom of pond in
At the bottom of culturing pool, culturing pool bottom perimeter is provided with 2-4 and is fixed on the gas stone at the bottom of culturing pool, directly over gas stone 1-2 li
Being perpendicular at the bottom of culturing pool be provided with a discharge at meter, discharge diameter is more than gas stone diameter, and discharge is by solid
Determining frame and be fixed on pool wall, turn to elbow one end to connect discharge top end opening, the other end is parallel and is less than the water surface
1-3cm, belongs to open type, turns to elbow consistent with water inlet pipe direction, gas stone and micro-pore oxygenation pipe pass through trachea and
Aerator is connected.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106538435A (en) * | 2017-01-06 | 2017-03-29 | 宁波大学 | A kind of winter grass shrimp constricta zoology cyclic culture method and device |
| CN109757422A (en) * | 2019-03-11 | 2019-05-17 | 山东睿洋水产科技有限公司 | Saline and alkaline underground water batch production water process cultivates Penaeus Vannmei equipment |
| RU2755309C1 (en) * | 2020-11-13 | 2021-09-15 | Общество с ограниченной ответственностью «Альготек Грин Технолоджи» (ООО «Эй-Джи-Ти») | Method for managing algoremediation of water bodies |
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| CN105875395B (en) | 2019-01-25 |
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