CN105861385A - Sulfadimidine degrading bacterium S-07 and screening method thereof - Google Patents

Sulfadimidine degrading bacterium S-07 and screening method thereof Download PDF

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Publication number
CN105861385A
CN105861385A CN201610345650.9A CN201610345650A CN105861385A CN 105861385 A CN105861385 A CN 105861385A CN 201610345650 A CN201610345650 A CN 201610345650A CN 105861385 A CN105861385 A CN 105861385A
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sulfadimidine
degradation bacteria
degradation
bacterium
sulfanilamide
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CN201610345650.9A
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汪印
潘兰佳
余广炜
唐晓达
李�杰
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Institute of Urban Environment of CAS
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Institute of Urban Environment of CAS
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    • C12N1/205
    • C12R2001/01
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F7/00Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36Adaptation or attenuation of cells
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention relates to a sulfadimidine degrading bacterium S-07 degraded by microorganisms and a screening method thereof. The sulfadimidine degrading bacterium S-07 is verified as Geobacillus, and the Genbank registration number of the bacterium 16S rDNA is KU588289. The sulfadimidine degrading bacterium is preserved in the China General Microbiological Culture Collection Center on April 21st, 2016, and the preservation number of the sulfadimidine degrading bacterium is CGMCC No. 12382. The sulfadimidine degrading bacterium S-07 well grows within the temperature range of 50-75 DEG C, has quite strong tolerance to sulfadimidine, and can efficiently degrade sulfadimidine in a liquid phase and a solid phase. The bacterium can be used for degrading and removing sulfadimidine residues in livestock excrement or sludge high-temperature compost, the removal rate is larger than 95%, and the sulfadimidine degrading bacterium S-07 has good industrial application prospects and environment benefits.

Description

One strain sulfadimidine degradation bacteria S-07 and screening technique thereof
Technical field
The present invention relates to environmental conservation and organic pollution microbial technology field, especially relate to a strain sulfanilamide two First pyrimidine degradation bacteria S-07 and screening technique thereof.
Background technology
Sulfa antibiotics is one of six big class antibiotic conventional in livestock and poultry breeding industry, is widely used in livestock and poultry animal disease Treatment and make an addition to feedstuff promotes the growth of animal.The Antibacterial Mechanism of sulfa antibiotics is: they are structurally similar Para-amino benzoic acid (PABA), can act on the dihydrofolate synthetase in bacterial body, thus stop PABA with PABA competitiveness As the process of folic acid required for Material synthesis antibacterial, decreasing the amount of the tetrahydrofolic acid with metabolic activity, the latter is then The required material of antibacterial synthetic DNA, therefore inhibits the growth and breeding of antibacterial.Sulfadimidine is conventional sulfa drugs One of, it is not fully absorbed through animal intestinal and is discharged by feces and urine with the form of original shape or metabolite. So, animal wastes have substantial amounts of Sulfamethazine Residues thing and exists.And animal wastes frequently as fertilizer application in Farm land, this other antibiotic medicine such as sulfadimidine resulting in residual enters in ecological environment, causes series of loops Border problem.Composting technology is considered as a kind of simple and easy to do method of decontamination of human excreta and recycling treatment, can effectively remove excrement Just most antibiotic remainss in.But sulfadimidine is the antibiotic that a class is more obstinate, and it is difficult at a temperature of conventional compostation Decompose, still suffer from Certain residues after compost maturation and fail to remove.
By the investigation to research both at home and abroad, find the bacterium that sulfa antibiotics is had degradation capability filtered out at present Great majority are room temperature or pyschrophile, it is impossible to be applied in During High-Temperature Composting.China Patent No.: ZL 201210153552.7, application public affairs Cloth day is on 03 06th, 2013, and invention and created name is: a strain Pseudomonas psychrophila HA-4 low temperature sulphur Amine methylisoxazole degradation bacteria and screening technique thereof and application.This bacterium can with sulfamethoxazole as sole carbon, nitrogen and energy Source grows, and its cellular liquid cultivates sulfamethoxazole of can degrading, it is possible to is applied to sulfamethoxine in municipal sewage and dislikes The biodegradation of azoles, the sulfamethoxazole clearance that initial concentration is 100 mg/L is reached by this bacterium under cryogenic 35.34 %.This bacterium is often/low temperature strain, it is impossible to be applied in During High-Temperature Composting, and this bacterium is to the degradation efficiency of antibiotic relatively Low.Therefore, it should filter out the high temperature bacterium that sulfa antibiotics is had efficient degradation ability, they are prepared as microbial inoculum application In actual During High-Temperature Composting, not only can improve the multiformity of microorganism in compost, improve compost temperature, it is also possible to make residual further The sulfonamide stayed thoroughly is degraded, and this has great importance for the environmental risk reducing compost product.
Summary of the invention
It is an object of the invention to provide strain sulfadimidine degradation bacteria S-07 and a screening technique thereof.The sulfanilamide filtered out Diformazan pyrimidine degradation bacteria S-07, can under high temperature (50-75 DEG C) efficient degradation sulfadimidine, microbial bacteria can be prepared as Agent, is applied in During High-Temperature Composting, it is achieved the efficient degradation of sulfadimidine antibiotic in composting production, for the agriculture of composting production Industry application lays the foundation.
Above-mentioned purpose and task are to be achieved through the following technical solutions:
One strain sulfadimidine degradation bacteria S-07, it is characterised in that: described sulfadimidine degradation bacteria S-07 is in 2016 Year is preserved in that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number is: CGMCC for 04 month 21 days No. 12382.Preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.Identified its is: happiness heat is addicted to oil ground bacillus (Geobacillus thermoleovorans).The Genbank accession number of the 16S rDNA of this bacterium is KU588289.
Further, described sulfadimidine degradation bacteria S-07 is accredited as ground Bacillus through 16s rRNA (Geobacillus) one, reaches 99.65 % with the homology of Geobacillus thermoleovorans.This bacterium 16S The Genbank accession number of rDNA is KU588289.
Further, described sulfadimidine degradation bacteria S-07 is from antibiotic pharmaceutical factory workshop mud sample at 70 DEG C Product, by enrichment, domestication with isolated and purified obtain.Specifically comprising the following steps that of screening technique
1) acclimating
Take workshop, the antibiotic pharmaceutical factory raw sewage that moisture content is 70-85 %, add sulfadimidine in the ratio of 20 ml/g Initial concentration is in the MMSM culture medium of 5.0 mg/L;Being 70 DEG C in temperature, shaking speed is lucifuge training under conditions of 100 rpm Support;Often after domestication 10 days, by medium centrifugal, the centrifugal precipitate obtained is forwarded in new MMSM culture medium, often transfers one Secondary, in MMSM culture medium, the concentration of sulfadimidine increases by 5 mg/L, until sulfadimidine concentration reaches 50 mg/L;
2) isolated and purified
Take culture fluid final for 0.1ml to coat on the selectivity flat board that sulfadimidine concentration is 50 mg/L, be placed in 70 DEG C Incubator is cultivated.The single bacterium colony grown on flat board is chosen, through repeatedly at the flat lining out of sulfadimidine selectivity, Isolated and purified sulfadimidine degradation bacteria S-07 that obtains, and rule to LB medium slant, it is placed in 4 DEG C of refrigerators and protects Hide.
Further, described MMSM culture medium consists of: Na2EDTA·2H2O (0.018 g/L), FeSO4·7H2O (0.013 g/L), CaCl2·2H2O (0.013 g/L), MgSO4·7H2O (0.25 g/L), Na2HPO4 (7.5 g/ L), KH2PO4(5 g/L), NH4NO3(5 g/L), yeast extract (0.6 g/L), glucose (0.5 g/L).
Further, the preparation method of described selectivity flat board is that the agar powder adding 2 % in MMSM culture medium shakes Even, in 121 DEG C of high-pressure sterilizing pots, sterilizing 20 minutes, it is cooled to 60-70 DEG C, is added thereto to sulfadimidine solution, shake up After pour in culture dish, prepare selectivity flat board after solidification.
Further, described LB culture medium consists of: tryptone (10.0 g/L), yeast extract (5.0 g/L), NaCl(10 g/L);Also can be added thereto to the agar powder of 2.0 %, make solid LB media.
Further, the degradation capability method of testing of described sulfadimidine is: used by the bacterium S-07 of slant preservation LB fluid medium activates, and makes seed liquor.Be seeded to the concentration of sulfadimidine be 10 mg/L, pH be 6.0 In MMSM culture medium, being placed in 70 DEG C, shaking speed is lucifuge cultivation under the conditions of 50 rpm.Record in fluid medium after 24 h The degradation rate of sulfadimidine is more than 90 %.
Further, described sulfadimidine degradation bacteria S-07 can be prepared as microbial bacterial agent and is applied to animal dung Just or in sludge high temperature compost, sulfadimidine therein of degrading.
Accompanying drawing explanation
Fig. 1 is sulfadimidine degradation bacteria S-07 form under scanning electron microscope.
Fig. 2 is that temperature is to the growth of sulfadimidine degradation bacteria S-07 and the shadow of degraded sulfadimidine ability Ring.
Fig. 3 is that pH is on the growth of sulfadimidine degradation bacteria S-07 and the impact of degraded sulfadimidine ability.
Detailed description of the invention
With embodiment, technical solution of the present invention is further elaborated below in conjunction with the accompanying drawings.
Embodiment 1
Weigh 5.0 workshop, g antibiotic pharmaceutical factory mud to be placed in 250 ml triangular flasks, wherein add 100 ml MMSM culture medium, Wherein the concentration of sulfadimidine is 5.0 mg/L, is placed in 70 DEG C, and shaking speed is lucifuge cultivation under the conditions of 100rpm.Often tame and docile Changing 10 days, take 5.0 ml culture fluid and transfer 1 time, in every subculture, antibiotic concentration increases by 5 mg/L, until sulfanilamide diformazan is phonetic Pyridine concentration reaches 50 mg/L.Take 0.1 ml culture fluid and coat the selectivity flat board that sulfadimidine concentration is 50mg/L, put Cultivate in 70 DEG C of incubators.The bacterium colony grown on flat board is chosen, isolated and purified obtains degradation bacteria S-07.
Wherein, MMSM culture medium preparation method is: Na2EDTA·2H2O (0.018 g/L), FeSO4·7H2O (0.013 g/L), CaCl2·2H2O (0.013 g/L), MgSO4·7H2O (0.25 g/L), Na2HPO4 (7.5 g/ L), KH2PO4(5 g/L), NH4NO3(5 g/L), yeast extract (0.6 g/L), glucose (0.5 g/L), sulfanilamide two First pyrimidine.Screening culture medium preparation method is: add the agar powder of 2.0% in MMSM culture medium, makes sulfadimidine and selects Property flat board.Culture medium is all in 121 DEG C of high-pressure sterilizing pots, and sterilizing used after 20 minutes.
Use 16s rRNA gene order method that degradation bacteria S-07 is carried out taxonomic identification.The 16s rRNA gene order recorded Comparing with the sequence reported in GenBank, result shows that degradation bacteria S-07 is for the one of ground bacillus (Geobacillus) Kind, this bacterium reaches 99.65 % with the homology of the Geobacillus thermoleovorans of ground Bacillus.
The temperature impact on degradation bacteria S-07 degradation capability
LB culture medium cultivates the seed liquor after 12 h, at room temperature 6000 rpm at 70 DEG C, is centrifuged 5 minutes, the precipitation obtained Thing PBS washs 2 times, then with normal saline, thalline is made OD600The bacteria suspension of=2.0.MMSM culture medium (pH= 6.5) in, the concentration of sulfadimidine is 10 mg/L, the bacteria suspension of 50 ml inoculation of medium 1.0 %, and shaking table temperature is respectively It is set as 50,55,60,65,70 and 75 DEG C, the concentration of sampling detection sulfadimidine after 24 h, and measure thalli growth amount (OD600), result is as shown in Figure 2.As seen from Figure 2, at 70 DEG C, the degradation effect of sulfadimidine is optimal.
The pH impact on degradation bacteria S-07 degradation capability
LB culture medium cultivates the seed liquor after 12 h, at room temperature 6000 rpm at 70 DEG C, is centrifuged 5 minutes, the precipitation obtained Thing PBS washs 2 times, then with normal saline, thalline is made OD600It it is the bacteria suspension of 2.0.Sulfanilamide in MMSM culture medium The concentration of diformazan pyrimidine is 10 mg/L, and with acid-alkali accommodation pH value be respectively 4.0,4.5,5.0,5.5,6.0,6.5,7.0, 7.5、8.0.The bacteria suspension of 50 ml inoculation of medium 1.0 %, shaking table temperature is set as 70 DEG C.Sampling and measuring sulfanilamide after 24 h The concentration of diformazan pyrimidine, and measure thalli growth amount (OD600).This bacterium is when pH=6.0 as seen from Figure 3, sulphur in culture medium The degradation rate of amine diformazan pyrimidine reaches maximum, and increment also reaches maximum.
The degradation bacteria S-07 degraded to sulfadimidine under the conditions of optimum temperature and pH
LB culture medium cultivates the seed liquor after 12 h, at room temperature 6000 rpm at 70 DEG C, is centrifuged 5 minutes, the precipitation obtained Thing PBS washs 2 times, then with normal saline, thalline is made OD600It it is the bacteria suspension of 2.0.Sulfanilamide in MMSM culture medium The concentration of diformazan pyrimidine is 10 mg/L, and with acid-alkali accommodation Medium's PH Value=6.0.Experimental group is at 50 ml inoculation of mediums The bacteria suspension of 1.0 %, matched group is then the sterilized water adding 1.0 % in 50 ml culture medium, and shaking table temperature is set as 70 DEG C, Rotating speed is 50 rpm.The concentration of the sulfadimidine of sampling and measuring matched group and experimental group after 24 h, and calculate corresponding Degradation rate.The degradation rate of matched group 24 h sulfadimidine is 23.35 %, the degraded of experimental group 24 h sulfadimidine Rate is up to 97.45 %.Removing the degradation effect of temperature, degradation bacteria S-07 reaches 74.10 to the degradation effect of sulfadimidine %。
Although above it has been shown and described that embodiments of the invention, it is to be understood that above-described embodiment is example Property, it is impossible to be interpreted as limitation of the present invention, those of ordinary skill in the art is without departing from the principle of the present invention and objective In the case of above-described embodiment can be changed within the scope of the invention, revise, replace and modification.

Claims (12)

1. strain sulfadimidine degradation bacteria S-07, it is characterised in that: described sulfadimidine degradation bacteria S-07 in Within on 04 21st, 2016, it is preserved in " China General Microbiological culture presevation administrative center ", its preserving number CGMCC No. 12382.
Strain sulfadimidine degradation bacteria S-07 the most according to claim 1, it is characterised in that: described sulfanilamide diformazan is phonetic Pyridine degradation bacteria S-07 is accredited as the one on ground Bacillus (Geobacillus) through 16s rRNA, with Geobacillus The homology of thermoleovorans reaches 99.65%.
Strain sulfadimidine degradation bacteria S-07 the most according to claim 1, it is characterised in that: described sulfanilamide diformazan is phonetic The Genbank accession number of the 16S rDNA of pyridine degradation bacteria S-07 is KU588289.
The screening technique of sulfadimidine degradation bacteria S-07 the most according to claim 1, it is characterised in that: described sulfanilamide Diformazan pyrimidine degradation bacteria S-07 is at 70 DEG C from antibiotic pharmaceutical factory workshop mud sample, by enrichment, tames and separates Purification obtains.
5. the specifically comprising the following steps that of screening technique
1) acclimating: take workshop, the antibiotic pharmaceutical factory raw sewage that moisture content is 70-85%, adds sulfanilamide in the ratio of 20 ml/g The initial concentration of diformazan pyrimidine is in the minimal medium (MMSM) of 5.0 mg/L;Being 70 DEG C in temperature, shaking speed is 100 Under conditions of rpm, lucifuge is cultivated;After often taming 10 days, by medium centrifugal, the centrifugal precipitate obtained is connected to new MMSM training Supporting in base, often transfer once, in MMSM culture medium, the concentration of sulfadimidine increases by 5 mg/L, until sulfadimidine is dense Degree reaches 50 mg/L;
2) isolated and purified: to take culture fluid final for 0.1 ml and coat selectivity that sulfadimidine concentration is 50 mg/L and put down On plate, it is placed in 70 DEG C of incubators cultivation.
Selectivity flat board the most according to claim 5, chooses the single bacterium colony grown on flat board, through repeatedly at sulfanilamide two The flat lining out of first pyrimidine selectivity, isolated and purified sulfadimidine degradation bacteria S-07 that obtains, and rule to LB cultivation On base inclined-plane, it is placed in 4 DEG C of Storage in refrigerator.
The screening technique of sulfadimidine degradation bacteria S-07 the most according to claim 5, it is characterised in that: described MMSM culture medium consists of: Na2EDTA·2H2O (0.018 g/L), FeSO4·7H2O (0.013 g/L), CaCl2· 2H2O (0.013 g/L), MgSO4·7H2O (0.25 g/L), Na2HPO4 (7.5 g/L), KH2PO4(5 g/L), NH4NO3(5 g/L), yeast extract (0.6 g/L), glucose (0.5 g/L).
The screening technique of sulfadimidine degradation bacteria S-07 the most according to claim 5, it is characterised in that: described choosing The preparation method of selecting property flat board is that the agar powder adding 2 % in MMSM culture medium shakes up, in 121 DEG C of high-pressure sterilizing pots, sterilizing 20 minutes, it is cooled to 60-70 DEG C, is added thereto to sulfadimidine solution, pour in culture dish after shaking up, prepare after solidification Go out selectivity flat board.
The screening technique of sulfadimidine degradation bacteria S-07 the most according to claim 5, it is characterised in that: described LB Culture medium consists of: tryptone (10.0 g/L), yeast extract (5.0 g/L), NaCl(10.0 g/L);Also can be wherein Add the agar powder of 2.0 %, make solid LB media.
10. according to sulfadimidine degradation bacteria S-07 described in claim 1,2 or 3, it is characterised in that: described sulfanilamide two The degradation capability method of testing of first pyrimidine is: is activated by the bacterium S-07 LB fluid medium of slant preservation, and makes kind Sub-liquid.
11. seed liquor according to claim 10, it is characterised in that: seed liquor is seeded to sulfadimidine concentration is 10 mg/L, pH are in the MMSM culture medium of 6.0, are placed in 70 DEG C, and shaking speed is lucifuge cultivation under the conditions of 50 rpm, after 24 h The degradation rate recording the sulfadimidine in fluid medium is more than 95 %.
12. according to sulfadimidine degradation bacteria S-07 described in claim 1,2 or 7, it is characterised in that: described sulfanilamide two First pyrimidine degradation bacteria S-07 can be prepared as microbial bacterial agent and is applied in feces of livestock and poultry or sludge high temperature compost, degrades therein Sulfadimidine.
CN201610345650.9A 2016-05-23 2016-05-23 Sulfadimidine degrading bacterium S-07 and screening method thereof Pending CN105861385A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108611285A (en) * 2018-04-04 2018-10-02 华南农业大学 A kind of sulfa antibiotics degradation bacteria and its application
CN108611286A (en) * 2018-04-04 2018-10-02 华南农业大学 A kind of sulfa antibiotics/heavy-metal composite pollution degradation/adhered bacteria and its application
CN108823115A (en) * 2018-05-15 2018-11-16 浙江省农业科学院 A kind of sulfa antibiotics degradation Alcaligenes and its application
CN109280631A (en) * 2018-10-24 2019-01-29 哈尔滨商业大学 One plant of sulfamethazine degradation bacteria S-2 and its application

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WO2013138928A1 (en) * 2012-03-23 2013-09-26 Himark Biogas Inc. Use of anaerobic digestion to destroy antibiotics in organic waste

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WO2013138928A1 (en) * 2012-03-23 2013-09-26 Himark Biogas Inc. Use of anaerobic digestion to destroy antibiotics in organic waste

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赵方: "磺胺二甲基嘧啶的微波与微生物降解研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108611285A (en) * 2018-04-04 2018-10-02 华南农业大学 A kind of sulfa antibiotics degradation bacteria and its application
CN108611286A (en) * 2018-04-04 2018-10-02 华南农业大学 A kind of sulfa antibiotics/heavy-metal composite pollution degradation/adhered bacteria and its application
CN108611285B (en) * 2018-04-04 2020-06-12 华南农业大学 Sulfonamide antibiotic degrading bacteria and application thereof
CN108611286B (en) * 2018-04-04 2020-08-07 华南农业大学 Sulfonamide antibiotic/heavy metal combined pollution degradation/adsorption bacterium and application thereof
CN108823115A (en) * 2018-05-15 2018-11-16 浙江省农业科学院 A kind of sulfa antibiotics degradation Alcaligenes and its application
CN108823115B (en) * 2018-05-15 2020-06-23 浙江省农业科学院 Sulfonamide antibiotic degradation alcaligenes and application thereof
CN109280631A (en) * 2018-10-24 2019-01-29 哈尔滨商业大学 One plant of sulfamethazine degradation bacteria S-2 and its application

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Application publication date: 20160817