CN105817275A - Centrifugal-type flow channel device - Google Patents
Centrifugal-type flow channel device Download PDFInfo
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- CN105817275A CN105817275A CN201510011267.5A CN201510011267A CN105817275A CN 105817275 A CN105817275 A CN 105817275A CN 201510011267 A CN201510011267 A CN 201510011267A CN 105817275 A CN105817275 A CN 105817275A
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Abstract
The invention discloses a centrifugal-type flow channel device. The centrifugal-type flow channel device comprises a flow channel body, a collection unit and a waste liquid bath. The flow channel body comprises a first surface and a second surface which are relatively configured. The flow channel body comprises a sample inlet, a sample flow channel, a separating tank, a reagent inlet, a reagent flow channel and a mixing channel. The sample inlet is configured on the first surface, the sample channel is connected with the sample inlet, the separating tank is arranged to the sample flow channel, and is communicated with the sample flow channel. The reagent inlet is configured on the first surface, the reagent flow channel is connected with the reagent inlet, and one end of the mixing flow channel is connected with the sample flow channel and the reagent flow channel. The collection unit comprises a perforate and at least an overflow orifice, the perforate is communicated with the other end of the mixing flow channel, and the waste liquid bath is communicated with the overflow orifice of the collection unit. According to the invention, a specimen sample flows through a junction of the sample flow channel and the separating tank, through the centrifugal force effect and screening by a density gradient solution, the substances in the specimen sample can be separated.
Description
Technical field
The present invention is about a kind of flow passage device, especially with regard to a kind of centrifugal flow passage device.
Background technology
No matter on clinical medicine, or on food test, all there is the demand of detection specific biological molecules.Specifically, it is on clinical medicine, by detecting the content of the various biomolecule of human body, such as the free cell in the body fluid such as blood, urine or the content of all types of protein, then can operate the most normal by each organ of entry evaluation human body.And on food test, more can be by the material in detection raw-food material or product, whether entry evaluation food contains the carcinogen of excess, pesticide residues or identifies gene transformation food etc..But, before detecting, isolated or purified (purify) must be carried out for the specific biological molecules in corpse or other object for laboratory examination and chemical testing sample (the most aforesaid blood, urine and raw-food material or product etc.) or material, to improve the concentration of specific subject matter, beneficially the carrying out of subsequent detection experiment.Therefore, the process of corpse or other object for laboratory examination and chemical testing sample with separate, be the primary goal that promotes of raw doctor's detection technique.
At present miniflow (microfluid) technology is applied the material in isolated or purified corpse or other object for laboratory examination and chemical testing sample, the most centrifugal (centrifugal-based) microfluidic devices.Centrifugal microfluidic devices is centrifuged by what rotation produced with the material in isolated or purified corpse or other object for laboratory examination and chemical testing sample.But, centrifugal microfluidic devices operationally, is only capable of injecting an a small amount of corpse or other object for laboratory examination and chemical testing every time, therefore is difficult to apply at the substantial amounts of corpse or other object for laboratory examination and chemical testing sample of process, also cannot detect the material that in corpse or other object for laboratory examination and chemical testing sample, content is relatively low.Therefore, centrifugal microfluidic device is fairly limited, normally only as research tool in business achievement.
Additionally, though there being the centrifugal microfluidic device after improvement at present, can be by the way of being continuously injected into, process substantial amounts of corpse or other object for laboratory examination and chemical testing sample, but it is for the corpse or other object for laboratory examination and chemical testing sample after the most separated, be provided only with passing away, so make separated after corpse or other object for laboratory examination and chemical testing sample be sprayed onto periphery because of centrifugal, be susceptible to the situation polluted.
Summary of the invention
Because above-mentioned problem, the purpose of the present invention is for providing a kind of centrifugal flow passage device, its material that separation weight or size differ by the way of centrifugal action collocation density gradient, and by collector unit and the design of waste liquid tank, to process substantial amounts of corpse or other object for laboratory examination and chemical testing sample easily, and can maintain the overall cleaning of centrifugal flow passage device simultaneously, and can avoid polluting and occur.
For reaching above-mentioned purpose, according to the centrifugal flow passage device of one of the present invention, including a runner body, at least one collector unit and a waste liquid tank.Runner body has a first surface and a second surface of relative configuration, and runner body includes a sample inlet, at least one sample runner, a separating tank, a reagent inlet, at least one reagent runner and at least one mixing runner.Sample inlet is configured at first surface.Sample runner connects sample inlet.Separating tank is adjacent to sample runner, and with sample flow passage.Reagent inlet is configured at first surface.Reagent runner connects reagent inlet.One end of mixing runner connects sample runner and reagent runner.Collector unit has a perforate and at least one spout hole, and perforate connects with the other end of mixing runner.Waste liquid tank connects with the spout hole of collector unit.
In one embodiment, the spout hole of collector unit is positioned at second surface, and waste liquid tank is connected to the second surface of runner body.
In one embodiment, the spout hole of collector unit is positioned at the outside of runner body, and waste liquid tank is connected to the outside of runner body.
In one embodiment, waste liquid tank is detachably arranged in runner body.
In one embodiment, waste liquid tank has a medial wall and a lateral wall, and this second surface of runner body has at least two snap fits, and the apical margin of medial wall and the apical margin of lateral wall respectively have at least one draw-in groove, and this two snap fit is sticked in this two draw-in groove.
In one embodiment, the second surface of runner body has at least two protuberances, forms to two sides extensions from snap fit, and with the outer peripheral edge around runner body, this two snap fit and this two protuberance are arranged at this two draw-in groove of this waste liquid tank jointly.
In one embodiment, runner body has more two elastic components, is arranged in this two draw-in groove.
In one embodiment, collector unit is detachably arranged in runner body.
In one embodiment, runner body has a cavity or an opening, and collector unit is arranged at cavity or opening.
In one embodiment, runner body has two barrel masts, is respectively arranged at sample inlet and the periphery of reagent inlet.
In one embodiment, runner body rounded video disc shape, waste liquid tank is annular groove, is arranged at the second surface of runner body, or around being arranged at the outside of runner body.
In one embodiment, sample runner local stretches out formation separating tank.
In one embodiment, sample inlet is arranged at the geometric center of runner body, and sample runner spirally stretches out.
For reaching above-mentioned purpose, according to the centrifugal flow passage device of one of the present invention, including a runner body, at least one collector unit and a waste liquid tank.Runner body has a first surface and a second surface of relative configuration, and runner body includes at least one sample runner, at least one reagent runner and at least one mixing runner.Sample inlet is arranged at the inside of runner body, and sample runner forms a sample inlet in first surface, and the local of sample runner stretches out formation one separating tank.Reagent runner is arranged at the inside of runner body, and reagent runner forms a reagent inlet in first surface.One end of mixing runner connects sample runner and reagent runner.Collector unit has a perforate and at least one spout hole, and perforate connects with the other end of mixing runner.Waste liquid tank connects with the spout hole of collector unit.
For reaching above-mentioned purpose, according to the centrifugal flow passage device of one of the present invention, including a runner body, a waste liquid tank and at least one collector unit.Runner body has a first surface and a second surface of relative configuration, and runner body includes a sample inlet, at least one sample runner, a separating tank, a reagent inlet, at least one reagent runner, at least one mixing runner and at least one first linking part.Sample inlet is configured at first surface.Sample runner connects sample inlet.Separating tank is adjacent to sample runner, and with sample flow passage.Reagent inlet is configured at first surface.Reagent runner connects reagent inlet.One end of mixing runner connects sample runner and reagent runner.First linking part is arranged at second surface.Waste liquid tank is detachably arranged in runner body, and waste liquid tank has at least one second linking part, the first linking part and the second linking part and cooperates.Collector unit has a perforate and at least one spout hole, and perforate connects with the other end of mixing runner, and spout hole connects with waste liquid tank.
In one embodiment, the first linking part is snap fit, and the second linking part is a draw-in groove.
In one embodiment, waste liquid tank has a medial wall and a lateral wall, and the second surface of runner body has at least two these snap fits, and the apical margin of medial wall and the apical margin of lateral wall respectively have at least one this draw-in groove, and this two snap fit is sticked in this two draw-in groove.
In one embodiment, the second surface of runner body has at least two protuberances, forms to two sides extensions from snap fit, and with the outer peripheral edge around runner body, this two snap fit and this two protuberance are arranged at this two draw-in groove of this waste liquid tank jointly.
In one embodiment, runner body has more two elastic components, is arranged in this two draw-in groove.
In one embodiment, runner body rounded video disc shape, waste liquid tank is annular groove, is arranged at the second surface of runner body, or around being arranged at the outside of runner body.
From the above, centrifugal flow passage device according to the present invention, it is by the design of runner, especially sample runner and the configuration relation of separating tank, when making the intersection that corpse or other object for laboratory examination and chemical testing sample flows through sample runner and separating tank, can be by the effect of centrifugal force and the screening of density gradient solution, the cell or the molecule that make lighter in weight flow to by the drive of centrifugal force mix runner, the cell of heavier-weight or molecule are then washed and Shen drops in separating tank, and then reach to separate effect of the material in corpse or other object for laboratory examination and chemical testing sample.
Also, the design that waste liquid tank connects with the spout hole of collector unit, make waste liquid tank can accept too much corpse or other object for laboratory examination and chemical testing sample, and then substantial amounts of corpse or other object for laboratory examination and chemical testing sample can be processed easily, and the cleaning of centrifugal flow passage device entirety can be maintained simultaneously, occur to avoid polluting.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of a kind of centrifugal flow passage device of first embodiment of the invention.
Fig. 2 is the sectional axonometric drawing of the centrifugal flow passage device shown in Fig. 1.
Fig. 3 is the runner schematic diagram of the centrifugal flow passage device shown in Fig. 1.
Fig. 4 is the collector unit shown in Fig. 2 and the enlarged diagram in portion of combined stream road.
Fig. 5 is the schematic diagram of a kind of centrifugal flow passage device of second embodiment of the invention.
Fig. 6 is the schematic diagram of a kind of centrifugal flow passage device of third embodiment of the invention.
Fig. 7 is the schematic diagram of a kind of centrifugal flow passage device of fourth embodiment of the invention
Fig. 8 A is the schematic diagram of a kind of centrifugal flow passage device of fifth embodiment of the invention.
Fig. 8 B is collector unit shown in Fig. 8 A and the enlarged diagram of part waste liquid tank.
Fig. 8 C is the collector unit shown in Fig. 8 B and waste liquid tank decomposing schematic representation.
Fig. 9 A is the schematic diagram of a kind of centrifugal flow passage device of sixth embodiment of the invention.
Fig. 9 B is the side view of the centrifugal flow passage device shown in Fig. 9 A.
Figure 10 A is the schematic diagram of a kind of centrifugal flow passage device of seventh embodiment of the invention.
Figure 10 B is the top view of the centrifugal flow passage device shown in Figure 10 A.
Figure 11 A is that the centrifugal flow passage device shown in Figure 10 A is in the profile of A-A hatching.
Figure 11 B is the decomposing schematic representation of the centrifugal flow passage device shown in Figure 11 A.
Figure 12 A is that the centrifugal flow passage device shown in Figure 10 A is in the profile of B-B hatching.
Figure 12 B is the decomposing schematic representation of the centrifugal flow passage device shown in Figure 12 A.
In figure, each description of reference numerals is as follows.
1,1a, 1b, 1c, 1d, 1e, 1f: runner body
10a, 10b, 10c: opening
11: first surface
12,12f: second surface
13,13a, 13b, 13c, 13d, 13e, 13f: sample inlet
14,14a, 14b, 14c, 14d, 14e, 14f: sample runner
15,15a, 15b, 15c, 15d, 15e, 15f: separating tank
16,16a, 16b, 16c, 16d, 16e, 16f: reagent inlet
17,17a, 17b, 17c, 17d, 17e, 17f: reagent runner
18,18a, 18b, 18c, 18d, 18e, 18f: mixing runner
181: confluence
182: micro structure
19e: barrel mast
2,2a, 2b, 2c, 2d, 2e, 2f: collector unit
21,21c, 21d: perforate
22,22c, 22d: spout hole
23,23c, 23d: accommodation space
3,3a, 3b, 3c, 3d, 3e, 3f: waste liquid tank
31: end face
32: protuberance
33d, 33f: medial wall
331f, 341f: draw-in groove
34f: lateral wall
A: low density area
B: high density area
C1, C2, C3, C4, C5, C6, C7: centrifugal flow passage device
H: perforate
HC: maxicell
LC: minicell
M1: first flow body
M11: groove
M11, M21: snap fit
M12, M22: protuberance
M2: the second runner body
O: hole, location
Detailed description of the invention
Hereinafter with reference to correlative type, a kind of centrifugal flow passage device according to present pre-ferred embodiments being described, the most identical element will be illustrated with identical reference marks.
Fig. 1 is the schematic diagram of a kind of centrifugal flow passage device of first embodiment of the invention, refer to shown in Fig. 1.The centrifugal flow passage device C1 of the present embodiment can be applicable to process different types of corpse or other object for laboratory examination and chemical testing sample, and corpse or other object for laboratory examination and chemical testing sample is fluid, and it can be such as but not limited to the biological sample sample of the flow-likes such as blood sample, plasma fluid, urine or other body fluid.Additionally, the centrifugal flow passage device C1 of the present embodiment more can be applicable in the corpse or other object for laboratory examination and chemical testing sample that examination target (specific biological molecules or material) concentration is relatively low, that is the examination target content that is present in corpse or other object for laboratory examination and chemical testing sample is less, therefore need to be by inputting substantial amounts of corpse or other object for laboratory examination and chemical testing sample, to obtain the examination target of q.s, and the centrifugal flow passage device C1 of the present embodiment i.e. can be applicable to process substantial amounts of corpse or other object for laboratory examination and chemical testing sample.
For example, during neoplasm metastasis (TumorMetastasis), i.e. refer to that tumor cell is from primary position, transfer to health other away from primary position (such as its hetero-organization or organ) continued growth during, tumor cell palpus adjacent cells the most Over or around, enter back in blood circulation, such as blood vessel, and its hetero-organization or organ it is transferred to by blood circulation, and tumor cell free in blood is referred to as " circulating tumor cell (circulatingtumorcells, CTC) ".Circulating tumor cell content in blood is low, that is, in blood sample, compared to other blood cell, it is low-down that circulating tumor cell accounts for the ratio of blood sample entirety composition, even if in there is the blood of sufferer of neoplasm metastasis, in every 106～109 mononuclear cells, generally only have a circulating tumor cell exist.Therefore, need to be by processing substantial amounts of blood sample (corpse or other object for laboratory examination and chemical testing sample), make to obtain the circulating tumor cell (examination target) of q.s, and the centrifugal flow passage device 1 of the present embodiment is explanation as a example by the circulating tumor cell to separate in blood sample.
Fig. 2 is the sectional axonometric drawing of the centrifugal flow passage device shown in Fig. 1, please also refer to shown in Fig. 1 and Fig. 2.Centrifugal flow passage device C1 includes runner body 1, an at least collector unit 2 and a waste liquid tank 3.The runner body 1 of the present embodiment in video disc shape, and can have first surface 11 and a second surface 12 of relative configuration, and specifically, if first surface 11 is referred to as front, second surface 12 is then the back side.It should be noted that, the visual angle presented due to Fig. 2, therefore second surface 12 is shown in the edge of second surface 12, and the back side that its indication person is runner body 1 (second surface 12), hereby chat bright.Structurally, runner body 1 includes sample inlet 13, at least sample runner 14, an at least separating tank 15, reagent inlet 16, at least reagent runner 17 and an at least one mixing runner 18.It should be noted that, the present invention does not limit sample runner 14, separating tank 15, reagent runner 17, mixing runner 18 and the quantity of collector unit 2, the explanation as a example by sample runner 14, separating tank 15, reagent runner 17, mixing runner 18 and a collector unit 2 of the present embodiment system.In other embodiments, can also multiple sample runners 14, separating tank 15, reagent runner 17, mixing runner 18 and collector unit 2, the present invention is not limited.
The runner body 1 of the present embodiment is in video disc shape, i.e. there is the video disc shape of thickness, make sample runner 14, separating tank 15, reagent runner 17 and mixing runner 18 can be formed at the inside of runner body 1, and sample inlet 13 and reagent inlet 16 are positioned at the first surface 11 of runner body 1.And the material of runner body 1 can be plastic material, such as polymethyl methacrylate (PMMA), Merlon (PC) or other thermoplastic.The diameter of runner body 1 can be between 6～18 centimeters, preferably 12 centimeters.
Specifically, sample inlet 13 is configured at first surface 11, that is has the sample inlet 13 of hatch frame in first surface 11, and sample runner 14 is formed at the inside of runner body 1 and connects sample inlet 13.It is also preferred that the left sample inlet 13 is positioned at the geometric center of runner body 1, it is more beneficial for during centrifugal flow passage device C1 operates, the injection blood sample of sustainability.It should be noted that, the be referred to as geometric center of the present embodiment is non-is a central point, but the region of neighbouring geometric center.Specifically, the centrifugal flow passage device C1 of the present embodiment can use with rotation platform collocation, drives centrifugal flow passage device C1 to rotate by rotation platform and produces the centrifugal force needed for separation corpse or other object for laboratory examination and chemical testing sample content thing.Centrifugal flow passage device C1 has more hole, the location O run through, and the present embodiment is as a example by three location hole O.Centrifugal flow passage device C1 can be fixed on rotation platform by location hole O, and when centrifugal flow passage device C1 is rotated by the driving of rotation platform, invariant position due to the geometric center of runner body 1, will not deviate because of rotation, therefore sustainability inject corpse or other object for laboratory examination and chemical testing sample in sample inlet 13, and then can be applicable to separate experiment or the detection method of a large amount of corpse or other object for laboratory examination and chemical testing sample.And the present invention is not intended to the configuration of runner body 1, it is only necessary to rotation that can be stable is to produce centrifugal force, and it is also preferred that the left runner body 1 can rounded video disc shape.
Same, reagent inlet 16 is configured at first surface 11, that is has the reagent inlet 16 of hatch frame in first surface 11, and reagent runner 17 is same in being arranged at the inside of runner body 1 and connecting reagent inlet 16.It is also preferred that the left reagent inlet 16 also can the geometric center of bit stream road body 1, that is the region of neighbouring geometric center.In the present embodiment, sample inlet 13 and reagent inlet 16 are same shape but different size, make sample inlet 13 and reagent inlet 16 can be simultaneously located at the geometric center of runner body 1, and the aspect that sample inlet 13 is in inner side, reagent inlet 16 is in outside.It is also preferred that the left sample inlet 13 and reagent inlet 16 are all circular open, and in the way of (concentric) with one heart, it is configured at the geometric center of runner body 1.Therefore, in addition to the injection corpse or other object for laboratory examination and chemical testing sample of sustainability, can also the injection reagent of persistence.Operationally, when centrifugal flow passage device C1 operates, corpse or other object for laboratory examination and chemical testing sample can be injected in sample inlet 13, such as blood sample, and reagent can be injected at reagent inlet 16 so that blood sample and reagent by the effect of centrifugal force, and can be loaded in sample runner 14 and reagent runner 17 respectively.
One end of the sample runner 14 of the present embodiment is connected to be arranged at the sample inlet 13 of the geometric center of runner body 1, and the other end is then spirally to stretch out, and to form the arcuate structure around sample inlet 13, and is configured at the inside of runner body 1.The inside of the runner body 1 of the present embodiment also has separating tank 15, and separating tank 15 is adjacent to sample runner 14, and connects with sample runner 14.Separating tank 15 also can be identical with sample runner 14, presents the arcuate structure around sample inlet 13.In the present embodiment, can be stretched out by the local of sample runner 14 and form separating tank 15, therefore the zone line of sample runner 14 forms, with separating tank 15, the aspect being interconnected.Specifically, it is narrower that sample runner 14 and the overall structure of separating tank 15 present two ends, and the non-uniform width that zone line is wider, i.e. narrower at sample inlet 13 and mixing runner 18, and zone line presents wider aspect because there being the structure of separating tank 15.Cell in blood sample can filter out, in sample runner 14 and separating tank 15, the cell that cell weight is lighter, and its detail content is in rear detailed description.
Same, reagent runner 17 can also present the arcuate structure around reagent inlet 16 by reagent inlet 16.And wherein one end of the mixing runner 18 of the present embodiment has more a confluence 181, to connect sample runner 14 and reagent runner 17, make separated after blood sample can enter mixed flow road 18 after confluxing in confluence 181 with reagent.The other end of mixing runner 18 connects with collector unit 2, and the mixed solution making blood sample and reagent is negotiable to collector unit 2, same, other detail content in rear detailed description it.
The separating tank 15 of the present embodiment is for housing density gradient solution, as a example by being the circulating tumor cell to separate in blood sample due to the present embodiment, therefore density gradient solution can be such as but not limited to Ficoll-Paque solution (Ficoll-paqueTMplusGEHealthcare).And density gradient solution first need to inject in separating tank 15 before injecting blood sample.And the period driven and rotate at runner body 1, density gradient solution can form density gradient in separating tank 15, and can screen the cell of Different Weight in blood sample by being formed of density gradient.
Fig. 3 is the runner schematic diagram of the centrifugal flow passage device shown in Fig. 1, utilizes the runner schematic diagram that Fig. 3 is drawn at this, illustrates to separate the situation of the circulating tumor cell in blood sample, please also refer to shown in Fig. 1 and Fig. 3.First, density gradient solution forms density gradient in separating tank 15 by the effect of centrifugal force, and the closer to the inner edge of runner body 1, density is relatively low, is referred to as low density area A at the present embodiment, and the closer to the outer rim of runner body 1, density is higher, is referred to as high density area B at the present embodiment.It should be noted that, separating tank 15 by the density gradient of inner edge to outer rim be continuous print from low to high, Fig. 3 is then for asking graphic simple and clear, directly divides into low density area A and high density area B.Additionally, for outline, blood sample comprises the cell (Heavycells) of heavier-weight and the cell (Lightcells) of lighter in weight, it is called maxicell HC and minicell LC in the present embodiment, wherein, maxicell HC such as erythrocyte, leukocyte etc., and minicell LC such as circulating tumor cell etc..When the period that runner body 1 is driven and rotate, centrifugal force can drive blood sample to flow (flow direction is as shown by arrows) from sample inlet 13 toward the lateral direction of runner body 1, reagent can be driven equally to flow toward the lateral direction of runner body 1 from reagent inlet 16, and the sample runner 14 respectively along helical form (or arc) flows with reagent runner 17.
In sample runner 14, when blood sample flows through the intersection of sample runner 14 and separating tank 15, subject to centrifugal forces and the screening of density gradient solution, minicell LC or little molecular suspension is in the A of low density area, and flow to further mix runner 18 by the drive of centrifugal force, maxicell HC or macromole are then washed and Shen drops in high density area B.In brief, the flow path of blood sample originates in sample inlet 13, flow along sample runner 14, its medium-small cells LC flows to by the effect of centrifugal force mix runner 18, maxicell HC is rested on the high density area B of separating tank 15 by centrifugal force and the screening of density gradient the most simultaneously, and then minicell LC is separated with maxicell HC, make the blood sample of entrance mixing runner 18 contains only minicell LC (including circulating tumor cell) and other little molecules.And after the blood sample after separated flows through mixing runner 18, enter back in collector unit 2, thereby reach to separate and collect the effect of the circulating tumor cell in blood sample.
In addition, in reagent runner 17, after reagent inlet 16 injects reagent, reagent can be made to enter mixed flow road 18 after flowing through reagent flow road 17 by the effect of centrifugal force, and then mixes with the blood sample after separated.Wherein, reagent can be buffer solution, or reagent has can the material of mark cycle tumor cell (i.e. examination target), and can the material of mark cycle tumor cell can be such as but not limited to fluorescent dye (fluorescentdye), antibody (antibody), immunity marker (immuno-marker) or magnetic bead (magneticbead) etc..Therefore, after circulating tumor cell can be labeled in mixing runner 18, enter collector unit 2, and circulating tumor cell also may be labeled in collector unit 2, thereby reach to detect the effect of the circulating tumor cell content in blood sample.
Fig. 4 is the collector unit shown in Fig. 2 and the enlarged diagram in portion of combined stream road, as shown in Figure 4, it is also preferred that the left mixing runner 18 more can have multiple micro structure 182, and the discontinuous inside being arranged at mixing runner 18 of those micro structures 182.The setting of micro structure 182 can increase reagent with separated after the mixability of blood sample, make that circulating tumor cell can be certain is labeled.It should be noted that, for seeking simplified form, micro structure 182 is only drawn in enlarged drawing.
Please also refer to shown in Fig. 2 and Fig. 4, the collector unit 2 of the present embodiment has perforate 21, an at least spout hole 22 and an accommodation space 23, perforate 21 connects with mixing runner 18, and waste liquid tank 3 connects with the spout hole 22 of collector unit 2, and accommodation space 23 is arranged between perforate 21 and spout hole 22.In the present embodiment, collector unit 2 is arranged at the outer peripheral edge of the runner body 1 of rounded video disc shape, and collector unit 2 is formed directly in the inside of runner body 1, and the spout hole 22 of collector unit 2 is positioned at the second surface 12 of runner body 1.It is also preferred that the left the waste liquid tank 3 of the present embodiment is annular groove, and it is connected to the second surface 12 of runner body 1, i.e. it is arranged at the lower section of runner body 1, and waste liquid tank 3 is formed directly in the second surface 12 of runner body 1, or being detachably arranged in runner body 1, the present invention is not limiting as.Therefore, the perforate 21 of collector unit 2 can receive separated and mixed blood sample from mixing runner 18, and be placed in accommodation space 23, and centrifugal flow passage device C1 can directly be moved to the device being available for observation by user, to observe separated and mixed blood sample (containing labeled circulating tumor cell).Also, owing to circulating tumor cell content in blood is rare, therefore when carrying out this type of experiment, substantial amounts of blood sample need to be injected, and when collector unit 2 collects too much blood sample, by spout hole 22, too much blood sample can be got rid of.Additionally, more can accept too much blood sample by the waste liquid tank 3 connected with spout hole 22, spray the contamination of surrounding avoiding directly getting rid of the caused blood sample of too much blood sample, thus can maintain centrifugal flow passage device C1 and with the use of the overall cleaning of experiment porch.
Preferably, as shown in Figure 2, the middle body of waste liquid tank 3 structure has further included an end face 31, and is provided with protuberance 32 on end face 31, and the position that arranges of protuberance 32 corresponding to the sample inlet 13 of runner body 1, sample runner 14, separating tank 15, reagent runner 17 and mixes the structures such as runner 18.It addition, protuberance 32 can be such as but not limited to o-ring (O-ring), when runner body 1 combines with waste liquid tank 3, to prevent the liquid flooding in aforementioned runner, can have effect of leakproof by the design of protuberance 32.
And the runner body 1 of the present embodiment, collector unit 2 and waste liquid tank 3 can have multiple different enforcement aspect, further illustrate with different embodiments below.
Fig. 5 is the schematic diagram of a kind of centrifugal flow passage device C2 of second embodiment of the invention, refer to shown in Fig. 5.In the present embodiment, collector unit 2a also can be to be detachably arranged in runner body 1a.Wherein, runner body 1a has a cavity or an opening, the present embodiment is as a example by opening 10a, collector unit 2a is made to be detachably arranged in opening 10a (or cavity), and the long axis direction of the opening 10a of the present embodiment is parallel with the long axis direction of runner body 1a, makes collector unit 2a can be placed in runner body 1a in the way of side slips into.Collector unit 2a is dismountable design, makes user can only take out collector unit 2a to observe.And the minutia of other elements of centrifugal flow passage device C2, can be not added with at this repeating directly with reference to the content described in centrifugal flow passage device C1 of first embodiment.
Additionally, Fig. 6 is the schematic diagram of a kind of centrifugal flow passage device C3 of third embodiment of the invention, refer to shown in Fig. 6, the long axis direction of the opening 10b of the present embodiment is vertical with the long axis direction of runner body 1a, makes the collector unit 2a can be to be directly placed in opening 10b from the top of runner body 1b.And the minutia of other elements of centrifugal flow passage device C3, content that can be directly described with reference to the centrifugal flow passage device C1 of first embodiment, it is not added with at this repeating.
The collector unit 2 (2a, 2b) of first, second and third embodiment is to be arranged at runner body 1 (1a, 1b) in the way of keeping flat, and in other embodiments, vertical mode can also be arranged at runner body.
Fig. 7 is the schematic diagram of a kind of centrifugal flow passage device C4 of fourth embodiment of the invention, as it is shown in fig. 7, the collector unit 2c of the present embodiment is to be arranged at runner body 1c in vertical manner.The perforate 21c of the collector unit 2c of the present embodiment is arranged at the upper limb of collector unit 2c, and spout hole 22c is then arranged at the lower edge of collector unit 2c, and runner body 1c correspondence has an opening 10c, and opening 10c runs through runner body 1c.During therefore collector unit 2c is placed in runner body 1c, the perforate 21c being positioned at collector unit 2c upper limb can connect with mixing runner 18c, and spout hole 22c can correspond directly to waste liquid tank 3c, makes waste liquid tank 3c can accept too much blood sample.And the minutia of other elements of centrifugal flow passage device C4, can be not added with at this repeating directly with reference to the content described in centrifugal flow passage device C1 of first embodiment.
Fig. 8 A is the schematic diagram of a kind of centrifugal flow passage device C5 of fifth embodiment of the invention, and Fig. 8 B is collector unit shown in Fig. 8 A and the enlarged diagram of part waste liquid tank, and Fig. 8 C is the collector unit shown in Fig. 8 B and waste liquid tank decomposing schematic representation.Please also refer to shown in Fig. 8 A to Fig. 8 C, the waste liquid tank 3d cincture of the present embodiment is arranged at the outside of runner body 1d, and the outside of the runner body 1d described in the present embodiment represents outside the sidewall of runner body 1d, as shown in Fig. 8 B and Fig. 8 C.The spout hole 22d of the collector unit 2d of the present embodiment is positioned at the outside of runner body 1d, i.e. it is arranged at the sidewall of runner body 1d, and the medial wall 33d of waste liquid tank 3d has the perforate H corresponding to spout hole 22d, waste liquid tank 3d is made to be connected to the outside of runner body 1d, waste liquid tank 3d is formed directly in the outside of runner body 1c, or it being detachably arranged in the outside of runner body 1d, the present invention is not limiting as.Therefore, spout hole 22d can connect with waste liquid tank 3d, and waste liquid tank 3d also can accept too much blood sample.Same, the minutia of other elements of centrifugal flow passage device C5, can be not added with at this repeating directly with reference to the content described in centrifugal flow passage device C1 of first embodiment.
Fig. 9 A is the schematic diagram of a kind of centrifugal flow passage device C6 of sixth embodiment of the invention, and Fig. 9 B is the side view of the centrifugal flow passage device shown in Fig. 9 A, please also refer to shown in Fig. 9 A and Fig. 9 B.The runner body 1e of the present embodiment more can have two barrel mast 19e, is respectively arranged at sample inlet 13e and the periphery of reagent inlet 16e.Specifically, the present embodiment is by raising up to be formed barricade at the periphery of sample inlet 13e and reagent inlet 16e, and barricade is the barrel mast 19e alleged by the present embodiment.The present embodiment can be by the setting of barrel mast 19e, with the contamination avoiding blood sample the most first to mix mutually with reagent before carrying out entering sample runner 14e.The minutia of other elements of centrifugal flow passage device C6, can be not added with at this repeating directly with reference to the content described in centrifugal flow passage device C1 of first embodiment.
Figure 10 A is the schematic diagram of a kind of centrifugal flow passage device of seventh embodiment of the invention, and Figure 10 B is the top view of the centrifugal flow passage device shown in Figure 10 A, refer to shown in Figure 10 A and Figure 10 B.The sample inlet 13f of the present embodiment, sample runner 14f, separating tank 15f, reagent inlet 16f, reagent runner 17f and mixing runner 18f are all arranged at runner body 1f, and collector unit 2f is also arranged at the outer peripheral edge of runner body 1f, and waste liquid tank 3f is detachably arranged in runner body 1f.In the present embodiment, runner body 1f is to be stacked by the sheet of two video disc shapes to form, and wherein, deserving to be called layer is first flow body M1, and lower floor is the second runner body M2.Those runners aforesaid and those inlets may be disposed at first flow body M1, and the sheet of lower floor is the most directly covered on the bottom side of the second runner body M2, can arrange in pairs or groups with reference to shown in Figure 11 A.
Figure 11 A be the centrifugal flow passage device shown in Figure 10 A in the profile of A-A hatching, Figure 11 B is the decomposing schematic representation of centrifugal flow passage device shown in Figure 11 A, please also refer to shown in Figure 11 A and Figure 11 B.At the second surface 12f of runner body 1f, there are at least two snap fit M11, M21, that is, snap fit M11 is formed at the bottom side of first flow body M1, and snap fit M21 is formed at the bottom side of the second runner body M2.Waste liquid tank 3f has an a medial wall 33f and lateral wall 34f, the apical margin of medial wall 33f respectively has at least one draw-in groove 331f, 341f with the apical margin of this lateral wall 34f, snap fit M11 is sticked in draw-in groove 341f, and snap fit M21 is sticked in draw-in groove 331f, and then waste liquid tank 3f is made to be detachably arranged in runner body 1f in the way of engaging.Therefore, more isometric waste liquid tank 3f can be selected according to the amount of capacity of injected blood sample.
As shown in Figure 10 B, the runner body 1f of the present embodiment, it is respectively provided with multiple snap fit M11 (or snap fit M21) interval and arranges, and have more protuberance M12 (or protuberance M22) between adjacent two snap fit M11 (or adjacent two snap fit M21).In other words, the second surface 12f of runner body 1f has at least two protuberance M12, M22, forms to two sides extensions from snap fit M11, M21 respectively, makes the common outer peripheral edge around runner body 1f of protuberance M12, M22 and snap fit M11, M21.Figure 12 A be the centrifugal flow passage device shown in Figure 10 A in the profile of B-B hatching, Figure 12 B is the decomposing schematic representation of the centrifugal flow passage device shown in Figure 12 A, please also refer to shown in Figure 12 A and Figure 12 B.And protuberance M12, M22 are similarly provided at draw-in groove 331f, 341f of waste liquid tank 3f, separately please arrange in pairs or groups shown in Figure 11 A, therefore snap fit M11, M21 of the present embodiment and protuberance M12, M22 are arranged at draw-in groove 331f, 341f jointly.And the configuration of draw-in groove 331f, 341f also can coordinate snap fit M11, M21 or the configuration of protuberance M12, M22 to be varied from, such as draw-in groove 331f, 341f can have breach, with the hook part (such as Figure 11 A) of accommodating snap fit M11, M21 corresponding to the position of snap fit M11, M21；Correspond to the position of protuberance M12, M22 then without structure jaggy.
Refer to shown in Figure 11 A and Figure 11 B, runner body 1f has more two hole, location O, the present embodiment system is as a example by o-ring, it is respectively arranged in draw-in groove 331f, 341f, and then be located between snap fit M11, M21 and draw-in groove 331f, 341f, and be located between protuberance M12, M22 and draw-in groove 331f, 341f (as illustrated in fig. 12), make runner body 1f can be combined more closely with waste liquid tank 3f, to reach effect of leakproof.
It addition, the present invention more proposes a kind of centrifugal flow passage device, it includes a runner body, at least one collector unit and a waste liquid tank.Runner body has a first surface and a second surface of relative configuration, and runner body includes sample runner, reagent runner and mixing runner, wherein, sample inlet is arranged at the inside of runner body, sample runner forms a sample inlet in first surface, and the local of sample runner stretches out formation one separating tank.Reagent runner is arranged at the inside of runner body, and reagent runner forms a reagent inlet in first surface.And connection relationship between sample inlet, separating tank and reagent inlet with other elements is identical with aforementioned first embodiment, therefore can be directly with reference to the runner body 1 of first embodiment.And when the spout hole of collector unit is positioned at second surface, waste liquid tank is then connected to the second surface (such as first embodiment) of runner body, and when the spout hole of collector unit is positioned at the outside of runner body, and waste liquid tank is then connected to the outside of runner body, such as the 4th embodiment, therefore the corresponding relation of collector unit and waste liquid tank can not repeat them here directly with reference to the centrifugal flow passage device C4 of the 4th embodiment.
The present invention proposes again a kind of centrifugal flow passage device, and it includes a runner body, a waste liquid tank and at least collector unit.Runner body has a first surface and a second surface of relative configuration, and runner body includes a sample inlet, at least one sample runner, a separating tank, a reagent inlet, at least one reagent runner, at least one mixing runner and at least one first linking part.Sample inlet is configured at first surface.Sample runner connects sample inlet.Separating tank is adjacent to sample runner, and with sample flow passage.Reagent inlet is configured at first surface.Reagent runner connects reagent inlet.One end of mixing runner connects sample runner and reagent runner.First linking part is arranged at second surface.Waste liquid tank is detachably arranged in runner body, and waste liquid tank has at least one second linking part, the first linking part and the second linking part and cooperates.Collector unit has a perforate and at least one spout hole, and perforate connects with the other end of mixing runner, and spout hole connects with waste liquid tank.
Wherein, the sample inlet of runner body, sample runner, separating tank, reagent inlet, reagent runner and mixing runner refer to previous embodiment, and the second surface of the runner body of the present embodiment is more provided with the first linking part, waste liquid tank has the second linking part of correspondence, first linking part and the second linking part cooperate, such as first linking part can be snap fit, and the second linking part can be draw-in groove.Therefore, the first linking part and the second linking part refer to the centrifugal flow passage device C7 of aforementioned 7th embodiment, and other element and detailed connection relationship and change aspect thereof refer to previous embodiment, do not repeat them here.
In sum, centrifugal flow passage device according to the present invention, it is by the design of runner, especially sample runner and the configuration relation of separating tank, when making the intersection that corpse or other object for laboratory examination and chemical testing sample flows through sample runner and separating tank, can be by the effect of centrifugal force and the screening of density gradient solution, the cell or the molecule that make lighter in weight flow to by the drive of centrifugal force mix runner, the cell of heavier-weight or molecule are then washed and Shen drops in separating tank, and then reach to separate effect of the material in corpse or other object for laboratory examination and chemical testing sample.
Also, the design that waste liquid tank connects with the spout hole of collector unit, make waste liquid tank can accept too much corpse or other object for laboratory examination and chemical testing sample, and then substantial amounts of corpse or other object for laboratory examination and chemical testing sample can be processed easily, and the cleaning of centrifugal flow passage device entirety can be maintained simultaneously, occur to avoid polluting.
The foregoing is only illustrative, rather than be restricted person.Any spirit and scope without departing from the present invention, and the equivalent modifications carrying out it or change, in claim attached after being intended to be limited solely by.
Claims (20)
1. a centrifugal flow passage device, it is characterised in that including:
One runner body, has a first surface and a second surface of relative configuration, and this runner body includes:
One sample inlet, is configured at this first surface；
At least one sample runner, connects this sample inlet；
At least one separating tank, is adjacent to this sample runner, and with this sample flow passage；
One reagent inlet, is configured at this first surface；
At least one reagent runner, connects this reagent inlet；And
At least one mixing runner, its one end connects this sample runner and this reagent runner；
At least one collector unit, has a perforate and at least one spout hole, and this perforate connects with the other end of this mixing runner；And
One waste liquid tank, connects with this spout hole of this collector unit.
Centrifugal flow passage device the most as claimed in claim 1, it is characterised in that this spout hole of this collector unit is positioned at this second surface, and this waste liquid tank is connected to this second surface of this runner body.
Centrifugal flow passage device the most as claimed in claim 1, it is characterised in that this spout hole of this collector unit is positioned at the outside of this runner body, and this waste liquid tank is connected to the outside of this runner body.
Centrifugal flow passage device the most as claimed in claim 1, it is characterised in that this waste liquid tank is detachably arranged in this runner body.
Centrifugal flow passage device the most as claimed in claim 4, it is characterized in that, this waste liquid tank has a medial wall and a lateral wall, and this second surface of this runner body has at least two snap fits, the apical margin of this medial wall and the apical margin of this lateral wall respectively have at least one draw-in groove, and this snap fit is sticked in this draw-in groove.
Centrifugal flow passage device the most as claimed in claim 5, it is characterized in that, this second surface of this runner body has at least two protuberances, forms to two sides extensions from this snap fit, with the outer peripheral edge around this runner body, this snap fit and this protuberance are arranged at this draw-in groove of this waste liquid tank.
Centrifugal flow passage device the most as claimed in claim 5, it is characterised in that this runner body has more two elastic components, is arranged in this two draw-in groove.
Centrifugal flow passage device the most as claimed in claim 1, it is characterised in that this collector unit is detachably arranged in this runner body.
Centrifugal flow passage device the most as claimed in claim 8, it is characterised in that this runner body has a cavity or an opening, this collector unit is arranged at this cavity or this opening.
Centrifugal flow passage device the most as claimed in claim 1, it is characterised in that this runner body has two barrel masts, is respectively arranged at this sample inlet and the periphery of this reagent inlet.
11. centrifugal flow passage devices as claimed in claim 1, it is characterised in that this runner body rounded video disc shape, this waste liquid tank is annular groove, is arranged at this second surface of this runner body, or around being arranged at the outside of this runner body.
12. centrifugal flow passage devices as claimed in claim 1, it is characterised in that this sample runner local stretches out and forms this separating tank.
13. centrifugal flow passage devices as claimed in claim 1, it is characterised in that this sample inlet is arranged at the geometric center of this runner body, and this sample runner spirally stretches out.
14. 1 kinds of centrifugal flow passage devices, it is characterised in that including:
One runner body, has a first surface and a second surface of relative configuration, and this runner body includes:
At least one sample runner, is arranged at the inside of this runner body, and this sample runner forms a sample inlet in this first surface, and the local of this sample runner stretches out formation one separating tank；
At least one reagent runner, is arranged at the inside of this runner body, and this reagent runner forms a reagent inlet in this first surface；And
At least one mixing runner, its one end connects this sample runner and this reagent runner；
At least one collector unit, has a perforate and at least one spout hole, and this perforate connects with the other end of this mixing runner；And
One waste liquid tank, connects with this spout hole of this collector unit.
15. 1 kinds of centrifugal flow passage devices, it is characterised in that including:
One runner body, has a first surface and a second surface of relative configuration, and this runner body includes:
One sample inlet, is configured at this first surface；
At least one sample runner, connects this sample inlet；
At least one separating tank, is adjacent to this sample runner, and with this sample flow passage；
One reagent inlet, is configured at this first surface；
At least one reagent runner, connects this reagent inlet；
At least one mixing runner, its one end connects this sample runner and this reagent runner, and the other end connects with this perforate of this collector unit；And
At least one first linking part, is arranged at this second surface；
One waste liquid tank, is detachably arranged in this runner body, and this waste liquid tank has at least one second linking part, and this first linking part cooperates with this second linking part；And
At least one collector unit, has a perforate and at least one spout hole, and this perforate connects with the other end of this mixing runner, and this spout hole connects with this waste liquid tank.
16. centrifugal flow passage devices as claimed in claim 15, it is characterised in that this first linking part is snap fit, this second linking part is a draw-in groove.
17. centrifugal flow passage devices as claimed in claim 16, it is characterized in that, this waste liquid tank has a medial wall and a lateral wall, this second surface of this runner body has at least two these snap fits, the apical margin of this medial wall and the apical margin of this lateral wall respectively have at least one this draw-in groove, and this two snap fit is sticked in this two draw-in groove.
18. centrifugal flow passage devices as claimed in claim 17, it is characterized in that, this second surface of this runner body has at least two protuberances, forms to two sides extensions from this snap fit, with the outer peripheral edge around this runner body, this two snap fit and this two protuberance are arranged at this two draw-in groove of this waste liquid tank jointly.
19. centrifugal flow passage devices as claimed in claim 16, it is characterised in that this runner body has more two elastic components, are arranged in this two draw-in groove.
20. centrifugal flow passage devices as claimed in claim 15, it is characterised in that this runner body rounded video disc shape, this waste liquid tank is annular groove, is arranged at this second surface of this runner body, or around being arranged at the outside of this runner body.
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| CN201510011267.5A CN105817275A (en) | 2015-01-09 | 2015-01-09 | Centrifugal-type flow channel device |
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| CN201510011267.5A CN105817275A (en) | 2015-01-09 | 2015-01-09 | Centrifugal-type flow channel device |
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