CN105806872A - Method for identifying different sturgeon roes by low field nuclear magnetism technology - Google Patents

Method for identifying different sturgeon roes by low field nuclear magnetism technology Download PDF

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CN105806872A
CN105806872A CN 201610280882 CN201610280882A CN105806872A CN 105806872 A CN105806872 A CN 105806872A CN 201610280882 CN201610280882 CN 201610280882 CN 201610280882 A CN201610280882 A CN 201610280882A CN 105806872 A CN105806872 A CN 105806872A
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sturgeon
identifying
roes
method
nuclear
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CN 201610280882
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Chinese (zh)
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谭明乾
黄琳琳
李永
周大勇
董秀萍
姜鹏飞
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大连工业大学
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N24/00Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
    • G01N24/08Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance

Abstract

The invention provides a method for identifying different sturgeon roes by a low field nuclear magnetism technology. The method comprises the following steps of collecting a plurality of 2-3.5mm different sturgeon roes as detecting samples in spring and autumn; performing low field nuclear magnetic resonance analysis on each sturgeon roe sample to obtain echo attenuationcurve data of sturgeon roe sample; performing principal component analysis on the obtained echo attenuation curve data, and building different sturgeon roe identifying models; performing low field nuclear magnetic resonance analysis on sturgeon roes to be tested by the same procedure as the second step, and identifying the varieties of the sturgeon roes to be tested by combining the sturgeon roe identifying models obtained in the third step. The method for identifying different sturgeon roes by the low field nuclear magnetism technology has the advantages that the great significance is realized in the aspects of reinforcing the trading circulation link technology supervision, protecting the sustainable development of Chinese special seafood product industry and improving the competitiveness of the Chinese seafood product in the international market; the method belongs to a very potential fast identifying new technology.

Description

利用低场核磁技术鉴别不同鲟鱼卵的方法 The method of using a low-field NMR techniques identify different roe of sturgeon

技术领域 FIELD

[0001]本发明涉及物质种类区分领域,具体来说,涉及利用低场核磁技术鉴别不同鋳鱼卵的方法。 [0001] The present invention relates to the field of the type-specific substances, in particular, a different method of low-field NMR techniques to identify eggs Zhu relates.

背景技术 Background technique

[0002]鋳鱼鱼卵是一种营养丰富的食品,其中有大量的蛋白质、胆固醇、维生素、核黄素、脑磷脂和钙、磷、铁等矿物质,是人类大脑和骨髓的良好补充剂、滋长剂,尤其对儿童生长发育极为重要,但又是我们日常膳食中比较容易缺乏的。 [0002] Zhu fish eggs are a nutrient-rich foods, including plenty of protein, cholesterol, vitamins, riboflavin, brain phospholipids and calcium, phosphorus, iron and other minerals, it is a good supplement human brain and bone marrow , grow agents, especially for children's growth and development is extremely important, but in our daily diet is easier lacking. 用2%〜5%的盐对鋳鱼卵进行腌渍发酵,形成市场上销售的鱼子酱。 With 2% ~ 5% of the eggs for pickling salt Zhu fermentation, is formed on the market caviar. 加盐腌渍后的鱼卵保质期延长,其中的水分、油脂、蛋白质等营养成分含量也有所改变。 The shelf life of the eggs with salt pickled extended, in which nutrition ingredient content of moisture, fat, protein, etc. have changed. 而对于不同种类的鋳鱼卵,其市场价格、营养价值也会有很大的不同。 As for the different types of Zhu eggs, its market price, nutritional value will be very different. 然而对消费者来说紧靠肉眼很难辨别鋳鱼卵的种类。 However, for consumers against the naked eye it is difficult to distinguish the type of Zhu roe. 因此发展快速、准确、便捷地鉴别鋳鱼卵等海产品技术,对于加强贸易流通环节技术监管,保护我国特色海产品产业可持续发展,提高我国海产品在国际市场上的竞争力有重大意义。 Therefore the development of fast, accurate and easily identify Zhu eggs and other seafood technology, to strengthen trade circulation technical supervision, protection of the sustainable development of the seafood industry characteristics, improve the competitiveness of our marine products in the international market has great significance.

[0003]目前,用于不同鋳鱼卵的检测方法主要包括感官评定、物理化学方法、分子生物学方法和光谱学方法。 [0003] Currently, a method for detecting different Zhu eggs mainly includes sensory evaluation, physical chemistry, molecular biology methods and spectroscopic methods. 感官评定和物理化学方法检测速度慢,且受主观因素的影响,使其在快速检测应用中受到限制。 Sensory evaluation and physical and chemical detection method is slow and influenced by subjective factors, it is limited in rapid detection applications. 而分子生物学和光谱学方法存在检测方法繁杂,步骤繁琐等缺陷。 The method of detecting the presence of complicated, cumbersome steps and other defects of molecular biology and spectroscopic methods.

发明内容 SUMMARY

[0004]目前市售鋳鱼卵产品品种多样、良莠不齐,本发明的目的在于提供一种简单、快速、准确、环保的方法对不同鋳鱼卵加以鉴别,利用低场核磁共振技术鉴别腌渍/未腌渍鋳鱼卵、西伯利亚鋳鱼卵/海博瑞鋳鱼卵等。 [0004] Zhu eggs currently marketed product variety, mixed, object of the present invention to provide a simple, rapid, accurate, and environmentally friendly way to be identified for different eggs Zhu, authentication using low field NMR technique pickling / No pickled eggs Zhu, Zhu Siberian roe / Haibo Rui Zhu eggs and so on.

[0005]为达到上述目的,本发明提供了一种利用低场核磁技术鉴别不同鋳鱼卵的方法,包括如下步骤: [0005] To achieve the above object, the present invention provides a low-field NMR techniques identify different Zhu eggs using the method, comprising the steps of:

[0006] S1、分春秋两季采集若干大小为2〜3.5毫米的不同鋳鱼卵作为检测样品; [0006] S1, a plurality of spring and autumn collection of different size 2~3.5 mm Zhu eggs as a test sample;

[0007] S2、对步骤SI得到的各鋳鱼卵样品进行低场核磁共振分析,利用CPMG脉冲序列法采集核磁共振回波信号,得到各鋳鱼卵样品的回波衰减曲线数据; [0007] S2, Zhu of eggs each sample obtained in step SI low-field NMR analysis, nuclear magnetic resonance echo signals are acquired using CPMG pulse sequence method, to obtain each of the echo decay curve Zhu eggs sample data;

[0008] 所述CPMG脉冲序列法采用的参数为:90度脉宽Pl:13ys, 180度脉宽P2:26ys,重复采样等待时间Tw: lOOO-lOOOOms,模拟增益RGl:[ 10到20,均为整数],数字增益DRGl: [2到5,均为整数],前置放大增益PRG: [I,2,3],NS:4,8,16,NECH: 1000-10000,接收机带宽SW: 100,200,300KHz,开始采样时间的控制参数RFD:0.002-0.05ms,时延DLl:0.1-0.5ms; [0008] The parameters used in the method of CPMG pulse sequence: 90 ° pulse Pl: 13ys, 180 degree pulse width P2: 26ys, resampling the waiting time Tw: lOOO-lOOOOms, analog gain RGl: [10 to 20 of each is an integer], the digital gain DRGl: [2 to 5 are integers], preamp gain PRG: [I, 2,3], NS: 4,8,16, NECH: 1000-10000, receiver bandwidth SW : control parameter 100,200,300KHz, start sampling time RFD: 0.002-0.05ms, delay DLl: 0.1-0.5ms;

[0009] S3、运用化学计量学方法,采用The Unscrambler统计分析软件对步骤S2得到的鋳鱼卵样品样品的回波衰减曲线数据进行主成分分析,建立不同鋳鱼卵区分模型; [0009] S3, chemometrics method, using statistical analysis software, The Unscrambler decay curve of the echo data sample in the sample Zhu eggs obtained in step S2 principal component analysis, to distinguish between different model Zhu eggs;

[0010] S4、取待测鋳鱼卵lg,采用与步骤S2相同的程序对所述待测鋳鱼卵进行低场核磁共振分析,得到所述待测鋳鱼卵的回波衰减曲线数据;对得到的所述待测鋳鱼卵的回波衰减曲线数据进行主成分分析,结合步骤S3得到的鋳鱼卵区分模型,鉴别待测鋳鱼卵的种类。 [0010] S4, taking eggs tested Zhu lg, using the same procedure as the step S2 Zhu eggs tested for low-field NMR analysis, the obtained test Zhu eggs echo decay curve data; Zhu the obtained measured echo decay curve data eggs principal component analysis, to distinguish eggs Zhu binding model obtained in step S3, the discrimination test Zhu type eggs.

[0011]优选方式下,所述鋳鱼卵采集自6〜11条鋳鱼。 [0011] Preferably embodiment, the eggs collected from 6~11 article Zhu Zhu fish.

[0012] 最优方式下,所述不同鋳鱼卵为海博瑞鋳鱼卵和西伯利亚鋳鱼卵; [0012] the best mode, the eggs of different Zhu Haibo Rui Zhu Zhu Siberia eggs and eggs;

[0013] 最优方式下,所述不同鋳鱼卵为经过浓度为3〜5wt%的盐水腌渍的鋳鱼卵和未经过腌渍的鋳鱼卵。 [0013] Under optimal way, as the different eggs through Zhu concentration 3~5wt% brine pickled eggs and Zhu Zhu not been pickled eggs.

[0014]根据采集样本的筛选,本发明可以实现对于腌渍/未腌渍鋳鱼卵的鉴别,对于不同种类鋳鱼的鋳鱼卵的鉴别;进一步优选,本发明可同时实现腌渍/未腌渍、不同种类鋳鱼等多差别的同时鉴别。 [0014] According to the screening of the collected samples, the present invention may be implemented for pickled / unsalted Zhu roe identification, authentication for different kinds Zhu Zhu fish roe; more preferably, the present invention can be achieved simultaneously pickled / unsalted, different identification of simultaneous multiple different types of Zhu fish.

[0015]本发明的有益之处在于: [0015] the benefits of the present invention:

[0016] 1、本发明涉及的检测方法操作过程简单,鋳鱼卵样品无需前处理,不需消耗有机试剂,重复性好,分析时间短,对鋳鱼卵无破坏,为非侵入式测量方法可对不同鋳鱼卵进行区分。 [0016] 1, the detection method of operation of the invention relates to a simple, Zhu eggs without sample pretreatment, without consuming organic reagent, reproducible, analysis time, no damage to the eggs Zhu, non-invasive measurement method Zhu can distinguish between different eggs.

[0017] 2、本发明利用低场核磁共振技术结合主成分分析(PCA)定性对不同鋳鱼卵进行鉴另IJ,与传统的检测方法不同,低场核磁共振检测前无需对样品进行特殊前处理,检测过程中耗时短,具有便捷、快速、准确、无污染、非侵入的特点,而且不受鉴别样品表面状态(如颜色、形状等)影响;且本发明方法可以同时实现腌渍情况、鋳鱼卵品种等多因素的鉴别。 [0017] 2, the present invention utilizes a low-field nuclear magnetic resonance analysis (PCA) for the characterization of different Zhu Kam another IJ eggs, the conventional detection methods, without special sample prior to low-field NMR detection Principal Component processing, less time consuming detection process, having a convenient, fast, accurate, non-polluting, non-invasive characteristics, but without discriminating the surface state of the sample (such as color, shape, etc.) on; and the method of the present invention may be implemented simultaneously pickled case, identification of multiple factors Zhu eggs varieties.

[0018] 3、腌渍会破坏鋳鱼卵的营养成分,比未经过腌渍的鋳鱼卵具有较长的保质期,便于运输。 [0018] 3, Zhu pickled eggs will destroy nutrients, has a longer shelf life than non-pickled after Zhu eggs, easy to transport. 本发明方法可以有效实现腌渍/未腌渍鋳鱼卵的鉴别,鉴别出经过腌渍和未经腌渍的鋳鱼卵可为消费者者提供出一个较好的食用期限判断,让消费者明确购买的鋳鱼卵是否经过腌渍加工,保护消费者权益。 The method can effectively achieve pickled / unsalted identify Zhu roe, pickled and not identified after Zhu pickled eggs can provide a better judge for consumers who expiry date, so that consumers clearly purchased Zhu whether pickled eggs after processing, to protect the interests of consumers.

[0019] 4、目前,用于不同鋳鱼卵的检测方法主要包括感官评定、物理化学方法等,这些方法能够实现不同鋳鱼卵的区分,但检测速度慢,且受主观因素的影响,对普通消费者来说很难通过上述方法辨别鋳鱼卵的种类。 [0019] 4, at present, different methods for the detection of eggs Zhu include sensory evaluation, physical and chemical methods, which method enables to distinguish between different Zhu eggs, but the slow detection speed, and is subject to subjective factors, for the average consumer is difficult to distinguish the type of Zhu eggs by the above method. 以西伯利亚鋳鱼卵和海博瑞鋳鱼卵为例,两种鋳鱼卵营养成分含量及体腔液的生化组成均有所差异,因而,市场价格也不同;本发明方法可以实现西伯利亚鋳鱼卵/海博瑞鋳鱼卵的快速鉴别。 Siberian Zhu Zhu Haibo Rui eggs and eggs, for example, two kinds of biochemical content and Zhu body cavity fluid nutrient composition eggs were all different, therefore, the market price is different; method of the present invention may be implemented Siberian Zhu eggs / Haibo Rui Zhu rapid identification of fish eggs. 为消费者提供保障,避免消费市场鱼目混珠的情况发生。 Case to provide protection for consumers against consumption of fish in the market place.

[0020]综上所述,本发明利用低场核磁技术鉴别不同鋳鱼卵的方法,对于加强贸易流通环节技术监管,保护我国特色海产品产业可持续发展,提高我国海产品在国际市场上的竞争力有重大意义,是一种非常有潜力的快速区分新技术。 [0020] In summary, the present invention is a method utilizing low-field NMR techniques to identify different Zhu eggs, for the technical supervision to strengthen trade circulation, protect our seafood industry characteristics of sustainable development, enhance our marine products in the international market competitiveness of great significance, is a very fast distinction between new technology potential.

附图说明 BRIEF DESCRIPTION

[0021 ]图1腌渍和未腌渍鋳鱼卵CPMG衰减曲线; [0021] FIGS. 1 and unsalted Zhu pickled eggs CPMG decay curve;

[0022]图2腌渍和未腌渍鋳鱼卵主成分散点图(CPMG序列); [0022] FIGS. 2 and pickled eggs unsalted Zhu scattergram main component (the CPMG sequence);

[0023] 图3不同种类鋳鱼卵CPMG衰减曲线; [0023] FIG 3 different types of eggs Zhu CPMG decay curve;

[0024]图4不同种类鋳鱼卵主成分散点图(CPMG序列)。 [0024] FIG 4 different types of eggs Zhu scattergram main component (the CPMG sequence).

具体实施方式 detailed description

[0025]以下结合附图和实施例,对本发明的具体实施方式进行更加详细的说明,以便能够更好地理解本发明的方案以及其各个方面的优点。 [0025] The following embodiments and the accompanying drawings, specific embodiments of the present invention will be explained in more detail, in order to better appreciate the advantages of the present invention and its various aspects. 以下描述的具体实施方式和实施例仅是说明的目的,而不是对本发明的限制。 DETAILED DESCRIPTION The following description and examples are merely illustrative purposes, and not to limit the present invention.

[0026]下述实施例中所用的材料、试剂等,如无特殊说明,均可从商业途径得到。 [0026] Example materials used, reagents and the like, no special instructions such as the following, can be obtained from commercial sources.

[0027] 实施例1 [0027] Example 1

[0028]利用低场核磁技术鉴别鋳鱼卵是否被腌渍加工的方法,包括如下步骤: [0028] using the method of low-field NMR techniques whether the authentication is pickled Zhu processed eggs, comprising the steps of:

[0029] S1、采集春秋两季海博瑞鋳鱼,取其直径在2.5到3毫米之间的鋳鱼卵。 [0029] S1, collected in spring and autumn Haibo Rui Zhu fish, eggs Zhu whichever diameter between 2.5 and 3 mm. 其中,未腌渍的海博瑞鋳鱼卵样本采自6条海博瑞鋳鱼,每条鱼采集三个样品,共计18个样品,腌渍的海博瑞鋳鱼卵样本采自11条海博瑞鋳鱼,每条鱼采集三个样品,共计33个样品,每个样品取Ig; Wherein the unsalted Haibo Rui Zhu eggs collected from the sample 6 Haibo Rui Zhu fish, each fish collecting three samples, a total of 18 samples, Zhu Haibo Rui pickled samples were collected from 11 eggs Haibo Zhu Rui fish, each fish collecting three samples, a total of 33 samples, each sample an Ig;

[0030]作为检测样品,上述样品具有一定代表性。 [0030] As a test sample, in the sample having a good representative.

[0031] S2、对步骤SI得到的各鋳鱼卵样品进行低场核磁共振分析:利用CPMG脉冲序列法采集核磁共振回波信号,采用Mini MR-Rat磁共振成像分析仪对上述所有鋳鱼卵样品进行低场核磁分析,利用CPMG脉冲序列,测量鋳鱼卵横向弛豫时间T2,参数设置为:90度脉宽Pl:13ys,180度脉宽P2: 26ys,重复采样等待时间Tw: 2000ms,模拟增益RGl: 20,数字增益DRGl:I,前置放大增益PRG: 3,NS: 8,NECH:6000,接收机带宽SW: 200KHz,开始采样时间的控制参数RFD: 0.008ms,时延DLl: 0.2ms,获得回波衰减曲线。 [0031] S2, Zhu of eggs each sample obtained in step SI low-field NMR analysis: NMR echo signals are acquired using CPMG pulse sequence method using magnetic resonance imaging Mini MR-Rat Analyzer all of the above Zhu eggs low-field NMR analysis of samples using CPMG pulse sequence, Zhu eggs measuring transverse relaxation time T2, the parameter is set to: 90 ° pulse Pl: 13ys, 180 degree pulse width P2: 26ys, resampling the waiting time Tw: 2000ms, the analog gain RGl: 20, digital gain DRGl: I, preamp gain PRG: 3, NS: 8, NECH: 6000, receiver bandwidth SW: 200KHz, the sampling start time control parameter RFD: 0.008ms, delay DLl: 0.2ms, the echo attenuation curve is obtained. 如图1所示,得到腌渍和未腌渍海博瑞鋳鱼卵样品的回波衰减曲线数据; 1, and pickled to give unsalted eggs Haibo Rui Zhu echo decay curve data of the sample;

[0032] S3、运用化学计量学方法,结合The Unscrambler软件,对步骤S2得到的各鋳鱼卵样品的回波衰减曲线数据进行主成分分析,建立鋳鱼卵是否被腌渍的区分模型,利用Origin软件绘图,如图2所示; [0032] S3, using chemometric methods, in conjunction with The Unscrambler software, the echo of each sample Zhu eggs step S2 decay curve data obtained by principal component analysis, the model distinguish whether Zhu eggs pickled using Origin drawing software, as shown in FIG 2;

[0033] S4、未知鋳鱼卵样品鉴别:取待测鋳鱼卵样品I,样品2各lg,进行上述步骤,鉴别待测鋳鱼卵是否被腌渍。 [0033] S4, the unknown sample identification Zhu eggs: eggs take samples tested Zhu I, each of Samples 2 lg, the above step, discriminating whether the test Zhu eggs being pickled. 结果如图2所示,样品I为未腌渍的海博瑞鋳鱼卵,样品2为腌渍的海博瑞鋳鱼卵。 The results shown in Figure 2, Sample I is a non-pickled eggs Zhu Haibo Rui, Sample 2 was pickled Haibo Rui Zhu eggs.

[0034] 实施例2 [0034] Example 2

[0035]利用低场核磁技术鉴别鋳鱼卵种类的方法,包括如下步骤: [0035] The use of low-field NMR technique is a method for identifying the type Zhu eggs, comprising the steps of:

[0036] Rl、采集春秋两季海博瑞鋳鱼,取其直径在2.5到3毫米之间的鋳鱼卵,其中,未腌渍的海博瑞鋳鱼卵样本采自6条海博瑞鋳鱼,每条鱼采集三个样品,共计18个样品,未腌渍的西伯利亚鋳鱼卵样本采自6条西伯利亚鋳鱼,每条鱼采集三个样品,共计18个样品,每个样品取Ig; [0036] Rl, in spring and autumn Haibo Rui Zhu collected fish, eggs Zhu whichever diameter between 2.5 and 3 mm, wherein the unsalted Haibo Rui Zhu eggs collected from the sample 6 Haibo Rui Zhu fish, each fish collecting three samples, a total of 18 samples, Siberian Zhu unsalted eggs collected from the sample 6 Siberian Zhu fish, each fish collecting three samples, a total of 18 samples, each sample an Ig;

[0037]作为检测样品,上述样品具有一定代表性。 [0037] As a test sample, in the sample having a good representative.

[0038] R2、对步骤Rl得到的各鋳鱼卵样品进行低场核磁共振分析:利用CPMG脉冲序列法采集核磁共振回波信号,采用Mini MR-Rat磁共振成像分析仪对上述所有鋳鱼卵样品进行低场核磁分析,利用CPMG脉冲序列,测量鋳鱼卵横向弛豫时间T2,参数设置为:90度脉宽Pl:13ys,180度脉宽P2: 26ys,重复采样等待时间Tw: 2000ms,模拟增益RGl: 20,数字增益DRGl:I,前置放大增益PRG: 3,NS: 8,NECH:6000,接收机带宽SW: 200KHz,开始采样时间的控制参数RFD: 0.008ms,时延DLl: 0.2ms,获得回波衰减曲线。 [0038] R2, Zhu of eggs each sample obtained in step Rl low field NMR analysis: NMR echo signals are acquired using CPMG pulse sequence method using magnetic resonance imaging Mini MR-Rat Analyzer all of the above Zhu eggs low-field NMR analysis of samples using CPMG pulse sequence, Zhu eggs measuring transverse relaxation time T2, the parameter is set to: 90 ° pulse Pl: 13ys, 180 degree pulse width P2: 26ys, resampling the waiting time Tw: 2000ms, the analog gain RGl: 20, digital gain DRGl: I, preamp gain PRG: 3, NS: 8, NECH: 6000, receiver bandwidth SW: 200KHz, the sampling start time control parameter RFD: 0.008ms, delay DLl: 0.2ms, the echo attenuation curve is obtained. 如图3所示,得到未腌渍海博瑞鋳鱼卵和未腌渍西伯利亚鋳鱼卵样品的回波衰减曲线数据; 3, to obtain eggs and unsalted Haibo Rui Zhu Zhu Siberian unsalted eggs echo decay curve data of the sample;

[0039] R3、运用化学计量学方法,结合The Unscrambler软件,对步骤R2得到的各鋳鱼卵样品的回波衰减曲线数据进行主成分分析,建立鋳鱼卵是否被腌渍的区分模型,利用Origin软件绘图,如图4所示; [0039] R3, chemometrics methods, in conjunction with The Unscrambler software, the echo of each sample Zhu eggs step R2 attenuation curve data obtained by principal component analysis, the model distinguish whether Zhu eggs pickled using Origin drawing software, shown in Figure 4;

[0040] R4、未知鋳鱼卵样品鉴别:取待测鋳鱼卵样品3,样品4各lg,进行上述步骤,鉴别待测鋳鱼卵的种类。 [0040] R4, identification of unknown samples eggs Zhu: Zhu eggs tested samples taken 3, 4 samples each of lg, the above step, the type of authentication to be tested Zhu roe. 结果如图4所示,样品3为西伯利亚鋳鱼卵,样品4为海博瑞鋳鱼卵。 The results shown in Figure 4, Sample 3 eggs Siberian Zhu, sample 4 is Haibo Rui Zhu eggs. [0041 ]本发明不仅局限于对腌渍海博瑞鋳鱼卵和未腌渍海博瑞鋳鱼卵,以及未腌渍海博瑞鋳鱼卵和未腌渍西伯利亚鋳鱼卵的鉴别。 [0041] The present invention is not limited to eggs and pickled Haibo Rui Zhu Zhu Haibo Rui unsalted eggs, and the eggs and unsalted Haibo Rui Zhu Zhu Siberia unsalted eggs authentication.

[0042]综上,利用低场核磁结合主成分分析法可以很好的对不同鋳鱼卵进行区分,对待测鋳鱼卵样品无破坏,操作简便,可提高检测速度。 [0042] In summary, the use of low-field NMR analysis and principal components can be good for distinguishing different Zhu eggs, eggs Zhu treated test sample without damage, easy to operate, the detection speed can be improved.

[0043]以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明披露的技术范围内,根据本发明的技术方案及其发明构思加以等同替换或改变,都应涵盖在本发明的保护范围之内。 [0043] The above are only the preferred specific embodiments of the invention, but the scope of the present invention is not limited thereto, any skilled in the art in the art within the technical scope disclosed in the present invention, according to the present invention its aspect of the inventive concept changes or equivalent replacements to be, shall fall within the scope of the present invention.

Claims (4)

  1. 1.一种利用低场核磁技术鉴别不同鋳鱼卵的方法,其特征在于,包括如下步骤: 51、分春秋两季采集若干大小为2〜3.5毫米的不同鋳鱼卵作为检测样品; 52、对步骤SI得到的各鋳鱼卵样品进行低场核磁共振分析,利用CPMG脉冲序列法采集核磁共振回波信号,得到各鋳鱼卵样品的回波衰减曲线数据; 所述CPMG脉冲序列法采用的参数为:90度脉宽Pl: 13ys,180度脉宽P2: 26ys,重复采样等待时间Tw:1000-10000ms,模拟增益1^1:[10到20,均为整数],数字增益01^1:[2到5,均为整数],前置放大增益?1^:[1,2,3],呢:4,8,16,陬01:1000-10000,接收机带宽邠:100,200,300KHz,开始采样时间的控制参数RFD:0.002-0.05ms,时延DLl:0.1-0.5ms; 53、运用化学计量学方法,采用The Unscrambler统计分析软件对步骤S2得到的鋳鱼卵样品样品的回波衰减曲线数据进行主成分分析,建立不同鋳鱼卵区分模型; 54、取 A different method of low-field NMR techniques of Zhu eggs using the identification, characterized by comprising the steps of: 51, a plurality of spring and autumn collection of different size 2~3.5 mm as a test sample Zhu eggs; 52, Zhu eggs for each sample obtained in step SI low-field NMR analysis, nuclear magnetic resonance echo signals are acquired using CPMG pulse sequence method, to obtain each of the echo decay curve Zhu eggs sample data; the method using CPMG pulse sequence of parameters were: 90 ° pulse Pl: 13ys, 180 degree pulse width P2: 26ys, resampling the waiting time Tw: 1000-10000ms, analog gain ^ 1 1: [10 to 20, are integers], 01 ^ 1 digital gain : [2-5, are integers], preamp gain 1 ^:? [1,2,3], it: 4,8,16, Zou 01: 1000-10000, receiver bandwidth Bin: 100,200 , 300KHz, began to control the parameters RFD sampling time: 0.002-0.05ms, delay DLl: 0.1-0.5ms; 53, using chemometric methods, the use of eggs Zhu sample sample the Unscrambler statistical analysis software to get the step S2 echo decay curve data principal component analysis, to distinguish between different model Zhu eggs; 54, taking 待测鋳鱼卵lg,采用与步骤S2相同的程序对所述待测鋳鱼卵进行低场核磁共振分析,得到所述待测鋳鱼卵的回波衰减曲线数据;对得到的所述待测鋳鱼卵的回波衰减曲线数据进行主成分分析,结合步骤S3得到的鋳鱼卵区分模型,鉴别待测鋳鱼卵的种类。 Zhu eggs tested lg, using the same procedure as in step S2 of the eggs tested Zhu for low field NMR analysis, the obtained test Zhu eggs echo decay curve data; said to be obtained Zhu measured echo decay curve data eggs principal component analysis, to distinguish eggs Zhu binding model obtained in step S3, the discrimination test Zhu type eggs.
  2. 2.根据权利要求1所述利用低场核磁技术鉴别不同鋳鱼卵的方法,其特征在于,所述鋳鱼卵采集自6〜11条鋳鱼。 The method according to one using low field NMR technique identification of eggs of different Zhu claim, wherein said article 6~11 Zhu Zhu eggs collected from the fish.
  3. 3.根据权利要求1或2所述利用低场核磁技术鉴别不同鋳鱼卵的方法,其特征在于,所述不同鋳鱼卵为海博瑞鋳鱼卵和西伯利亚鋳鱼卵。 3. The method of claim 1 or 2, using a different low-field NMR techniques identify eggs Zhu claim, wherein said different Zhu eggs and eggs of Siberian Haibo Rui Zhu Zhu eggs.
  4. 4.根据权利要求1或2所述利用低场核磁技术鉴别不同鋳鱼卵的方法,其特征在于,所述不同鋳鱼卵为经过浓度为3〜5wt %的盐水腌渍的鋳鱼卵和未经过腌渍的鋳鱼卵。 1 or 2 according to the authentication technology using low field NMR method Zhu eggs of different claims, characterized in that, after the different concentration of eggs Zhu 3~5wt% saline eggs and not pickled Zhu after Zhu pickled eggs.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009023054A1 (en) * 2007-05-18 2009-02-19 Los Alamos National Security, Llc Ultra-low field nuclear magnetic resonance and magnetic resonance imaging to discriminate and identify materials
US20100219827A1 (en) * 2007-05-18 2010-09-02 Los Alamos National Security, Llc Ultra-low field nuclear magnetic resonance and magnetic resonance imaging to discriminate and identify materials
CN101975788A (en) * 2010-09-01 2011-02-16 苏州纽迈电子科技有限公司;上海理工大学;上海纽迈电子科技有限公司 Method for identifying quality of edible oil with low-field NMR (Nuclear Magnetic Resonance)
WO2013062260A1 (en) * 2011-10-26 2013-05-02 한국표준과학연구원 Method and apparatus for identifying extremely-low-magnetic-field nuclear magnetic resonance material
CN104950005A (en) * 2014-03-26 2015-09-30 中国科学院大连化学物理研究所 Qualitative analysis method for distinguishing water contents of lightly dried sea cucumber, salt dried sea cucumber and expanded sea cucumber

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009023054A1 (en) * 2007-05-18 2009-02-19 Los Alamos National Security, Llc Ultra-low field nuclear magnetic resonance and magnetic resonance imaging to discriminate and identify materials
US20100219827A1 (en) * 2007-05-18 2010-09-02 Los Alamos National Security, Llc Ultra-low field nuclear magnetic resonance and magnetic resonance imaging to discriminate and identify materials
CN101975788A (en) * 2010-09-01 2011-02-16 苏州纽迈电子科技有限公司;上海理工大学;上海纽迈电子科技有限公司 Method for identifying quality of edible oil with low-field NMR (Nuclear Magnetic Resonance)
WO2013062260A1 (en) * 2011-10-26 2013-05-02 한국표준과학연구원 Method and apparatus for identifying extremely-low-magnetic-field nuclear magnetic resonance material
CN104950005A (en) * 2014-03-26 2015-09-30 中国科学院大连化学物理研究所 Qualitative analysis method for distinguishing water contents of lightly dried sea cucumber, salt dried sea cucumber and expanded sea cucumber

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
姜潮,韩剑众: "基于低场核磁共振技术的大米品种快速鉴别", 《食品工业科技》 *

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