CN105802851A - Dynamic tilted rotation bioreactor for 3D printing skin - Google Patents
Dynamic tilted rotation bioreactor for 3D printing skin Download PDFInfo
- Publication number
- CN105802851A CN105802851A CN201610176759.4A CN201610176759A CN105802851A CN 105802851 A CN105802851 A CN 105802851A CN 201610176759 A CN201610176759 A CN 201610176759A CN 105802851 A CN105802851 A CN 105802851A
- Authority
- CN
- China
- Prior art keywords
- skin
- culture dish
- main shaft
- ball
- fixed disc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
Abstract
The invention discloses a dynamic tilted rotation bioreactor for 3D printing skin.The bioreactor comprises a culture dish and a main shaft.The culture dish is connected with a rotating shaft through a fixed disc.The rotating shaft is connected with a base through a rubber ball.One end of the main shaft is connected with the fixed disc, and the other end of the main shaft penetrates through the center of the rubber ball and the rotating shaft to be connected with an engine on the base.The engine drives the main shaft to rotate in a tilted mode, up-down vibration of the rotating shaft is controlled through the rubber ball, and therefore tilted rotation and up-down vibration of the culture dish on the fixed disc are achieved.When the culture dish moves together with the fixed disc, due to the fact that inclination and up-down vibration enable upper-side tissue engineering skin to be exposed in air, the purpose of gas-liquid exchange culture is achieved, horn cells absorb nutritional ingredients from the bottom layer, differentiation and propulsion are continuously carried out towards the surface layer, a horny layer is formed, the epidermis is continuously promoted to be mature, the printed 3D printing skin is of a tissue-like structure, and an extracellular matrix similar to normal skin is produced.
Description
Technical field
The present invention relates to field of tissue engineering technology, the dynamic tilt rotating biological that a kind of 3D prints skin is anti-
Answer device.
Background technology
Skin refers to that body surface wraps in the tissue outside muscle, is the organ of human body maximum.Skin total weight accounts for body
The 5%～15% of weight, the gross area is 1.5～2 square metres, and thickness is different because of people or position, is 0.5 millimeter～4
Millimeter.Skin cover whole body, it make internal various tissue and organ from physical property, mechanicalness, chemical and
The invasion and attack of pathogenic microorganism.Skin has the barrier action of two aspects: on the one hand prevent internal moisture content, electricity
Xie Zhi, other materials are lost；On the other hand the intrusion of extraneous harmful substance is stoped.Skin remains human body internal ring
Stablizing of border, skin also assists in the metabolic process of human body simultaneously.When causing due to the factor such as environmental damage or disease
During skin injury, its harm can be slight, it is also possible to is fatal.
The reparation of skin wound and healing are the focus studied of surgery and difficult point.Especially for serious burn,
The refractory wound such as avulsion, diabetic foot and tumor ulceration on a large scale, the thorny of the traumatology department field is asked especially
Topic.The skin injury restorative procedure that tradition is commonly used has: auto-skin grafting, allogeneic skin graft, xenogenesis
Skin grafting dermepenthesis, but these methods all also exist for district's deficiency, immunologic rejection, the risk of pathophoresis, for solving this
A little difficult problems, find a kind of preferably Graftskin and are particularly important clinically.In recent years, 3D is biological
Printing technique is a dark horse in Manufacturing Engineering field, and starts to extend to biomedical sector.From initial
Universalness makes referrals to the desk study in research field, and in the short period of time, this technology is for solving donor skin
Skin area is limited and brings new dawn for skin wound reparation, and therefore 3D prints skin technology becomes
Research emphasis new in repair in trauma treatment field.
Prepare by 3D biometric print technology and may be used for clinical treatment containing cell, extracellular matrix etc.,
The skin of simulation natural component, and printing skin is exposed to air fluid level, in such a case, horn cell
Absorb nutritional labeling from bottom, constantly advance to top layer differentiation and form horny layer and constantly promote epidermal maturation (angle
Cell plastid and fibroblast), and one makes the skin printed be in the biological anti-of gas-liquid exchange plane
Device is answered to be particularly important.Existing bioreactor not only structure is complicated, and cannot make organization engineering skin
Complex grows under the growing environment of simulation human body skin, it is impossible to obtains and can really replace human body in performance
The organization engineering skin of skin.
Summary of the invention
The technical problem to be solved in the present invention is to overcome existing bioreactor construction complexity but can not make skin
It is in the defect of gas-liquid exchange plane, it is provided that a kind of 3D prints the dynamic tilt curled curved surface of skin.
In order to solve above-mentioned technical problem, the invention provides following technical scheme:
A kind of 3D prints the dynamic tilt curled curved surface of skin, including culture dish and main shaft, described training
Supporting ware to be connected with rotary shaft by fixed disk, described rotary shaft is by ball with the base with electromotor even
Connecing, described main shaft one end is connected with described fixed disk, and the other end passes the center of described ball and described rotation
Axle is connected with the electromotor on base.
Further, different size of culture dish can be fixed on described fixed disk by elastic cord.
Ball and base are fixing for fixed point, and ball can swing according to the rotary motion of rotary shaft, it is possible to
With according to programming with main shaft vertical tremor.Ball is attached by main shaft with rotary shaft, and ball rises
Cushioning effect in motor process；
Driven by engine inclined main axes on base rotates in the present invention, and the electromotor on base passes through ball
Control the vertical tremor of rotary shaft, thus realize the culture dish Sloped rotating on fixed disk and vertical tremor.Work as training
Support ware along with fixed disk move together time, due to tilt and vertical tremor can make upside organization engineering skin be combined
Thing is exposed in the middle of air, and reaches the purpose that solution-air exchange is cultivated so that horn cell absorbs battalion from bottom
Form point, constantly advance to top layer differentiation and form horny layer and constantly promote epidermal maturation, and make to print
3D prints skin and possesses " class loading " structure, produces the extracellular matrix of similar normal skin.With existing skill
Common bioreactor in art is compared, so that culture fluid enters material internal along piece of tissue all directions,
Reach to exchange nutrient substance, take the effect of Cellular respiration refuse out of, prevent " hollow " of the external cultivation of composite
Phenomenon；On the other hand the bioreactor of the present invention pick and place very convenient, and go for a certain size
In the range of culture vessel.
Accompanying drawing explanation
Accompanying drawing is for providing a further understanding of the present invention, and constitutes a part for description, with the present invention
Embodiment be used for together explaining the present invention, be not intended that limitation of the present invention.In the accompanying drawings:
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the schematic diagram of the operation principle of the main shaft drive fixed disk Sloped rotating of the present invention；
Fig. 3 is main shaft and the operation principle of ball drive fixed disk vertical tremor of the Sloped rotating of the present invention
Schematic diagram.
Detailed description of the invention
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are illustrated, it will be appreciated that described herein excellent
Select embodiment to be merely to illustrate and explain the present invention, being not intended to limit the present invention.
Embodiment
As Figure 1-3, a kind of 3D prints the dynamic tilt curled curved surface of skin, including culture dish
6 and main shaft 5, described culture dish 6 is connected with rotary shaft 2 by fixed disk 1, and rotary shaft 2 passes through ball
3 are connected with the base 4 with electromotor, and main shaft 5 one end is connected with fixed disk 1, and the other end passes ball
Center and the rotary shaft 2 of 3 are connected with the electromotor on base 4.
Culture dish 6 is fixed on fixed disk 1 by elastic cord.Ball 3 and base 4 are fixing for fixed point.
The Sloped rotating of the driven by engine main shaft 5 of base 4 in the present embodiment, and controlled by ball 3
The vertical tremor of rotary shaft 2, thus drive the culture dish 6 on fixed disk and fixed disk 1 Sloped rotating and on
Lower vibrations.Main shaft 5 one end is connected with the center of fixed disk 1, and one end is connected with the electromotor of base 4, passes
Ball 3 and rotary shaft 2, drive ball 3, rotary shaft 2, fixed disk 1 and the motion of culture dish 6；
It is fixing that ball 3 and base 4 carry out fixed point, and ball 3 can swing according to the rotary motion of rotary shaft 2,
Can also be according to programming with main shaft 5 vertical tremor；Ball 3 is carried out even by main shaft 5 with rotary shaft 2
Connecing, ball 3 plays the cushioning effect in motor process；It addition, the existence of ball 3 makes rotary shaft 2
Can be in certain scope, with certain frequency vertical tremor together with fixed disk 1；The upper end of rotary shaft 2
Be fixedly connected with fixed disk 1, i.e. the motion of rotary shaft 2 drives the motion of fixed disk 1.Described fixed disk
Can be fixed different size of culture dish 6 by elastic cord on 1, culture dish 6 is along with fixed disk 1 together
During motion, the organization engineering skin complex of upside can be made owing to tilting to be exposed in the middle of air with vertical tremor,
And reach the purpose that solution-air exchange is cultivated.
In practical operation, first culture dish 6 is arranged on fixed disk 1, runs machine.Ball 3 and rotation
Rotating shaft 2 drives fixed disk 1 and culture dish 6 to do Sloped rotating and vertical tremor motion, makes the organizational project of upside
Skin complex is exposed in the middle of air, and reaches the purpose that solution-air exchange is cultivated so that top layer horn cell
Differentiation advances and forms horny layer and constantly promote epidermal maturation, makes the 3D printed print skin and possesses " class
Tissue " structure, produce the extracellular matrix of similar normal skin；And make culture fluid can along piece of tissue each
Direction enters material internal, reaches to exchange nutrient substance, takes the effect of Cellular respiration refuse out of, prevent composite wood
" hollow " phenomenon of material In vitro culture.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to this
Invention, although being described in detail the present invention with reference to previous embodiment, for those skilled in the art
For, the technical scheme described in foregoing embodiments still can be modified by it, or to wherein part
Technical characteristic carries out equivalent.All within the spirit and principles in the present invention, any amendment of being made, equivalent
Replacement, improvement etc., should be included within the scope of the present invention.
Claims (3)
1. the dynamic tilt curled curved surface of a 3D printing skin, it is characterised in that include culture dish
(6) and main shaft (5), described culture dish (6) is connected with rotary shaft (2) by fixed disk (1), described
Rotary shaft (2) is connected with the base (4) with electromotor by ball (3), described main shaft (5)
End is connected with described fixed disk (1), and the other end passes center and the described rotary shaft (2) of described ball (3)
It is connected with the electromotor on base (4).
2. 3D prints the dynamic tilt curled curved surface of skin as claimed in claim 1, it is characterised in that
Described culture dish (6) is fixed on described fixed disk (1) by elastic cord.
3. 3D prints the dynamic tilt curled curved surface of skin as claimed in claim 1, it is characterised in that
Described ball (3) and base (4) are fixing for fixed point.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610176759.4A CN105802851B (en) | 2016-03-25 | 2016-03-25 | A kind of dynamic tilt curled curved surface of 3D printing skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610176759.4A CN105802851B (en) | 2016-03-25 | 2016-03-25 | A kind of dynamic tilt curled curved surface of 3D printing skin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105802851A true CN105802851A (en) | 2016-07-27 |
| CN105802851B CN105802851B (en) | 2018-06-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610176759.4A Active CN105802851B (en) | 2016-03-25 | 2016-03-25 | A kind of dynamic tilt curled curved surface of 3D printing skin |
Country Status (1)
| Country | Link |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639160A (en) * | 1995-03-15 | 1997-06-17 | Kishimoto; Masaru | Platform shaker in three dimensional motion |
| US6544788B2 (en) * | 2001-02-15 | 2003-04-08 | Vijay Singh | Disposable perfusion bioreactor for cell culture |
| CN201713522U (en) * | 2010-04-30 | 2011-01-19 | 惠倪 | Swinging cell reactor with polygonal plastic bag |
| CN102086438A (en) * | 2009-12-07 | 2011-06-08 | 韩春茂 | Device and method for biological culture of cell or tissue engineering |
| CN102482633A (en) * | 2009-07-06 | 2012-05-30 | 弗·哈夫曼-拉罗切有限公司 | Method of culturing eukaryotic cells |
-
2016
- 2016-03-25 CN CN201610176759.4A patent/CN105802851B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639160A (en) * | 1995-03-15 | 1997-06-17 | Kishimoto; Masaru | Platform shaker in three dimensional motion |
| US6544788B2 (en) * | 2001-02-15 | 2003-04-08 | Vijay Singh | Disposable perfusion bioreactor for cell culture |
| CN102482633A (en) * | 2009-07-06 | 2012-05-30 | 弗·哈夫曼-拉罗切有限公司 | Method of culturing eukaryotic cells |
| CN102086438A (en) * | 2009-12-07 | 2011-06-08 | 韩春茂 | Device and method for biological culture of cell or tissue engineering |
| CN201713522U (en) * | 2010-04-30 | 2011-01-19 | 惠倪 | Swinging cell reactor with polygonal plastic bag |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105802851B (en) | 2018-06-19 |
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Effective date of registration: 20180516 Address after: 215000 room 605, B1 building, biological nano Park, 218 Xing Hu Street, Suzhou Industrial Park, Suzhou, Jiangsu. Applicant after: Suzhou nuopu Regenerative Medicine Co. Ltd. Address before: 214000 -3, 130 Meishan Road, Mashan, Wuxi, Binhu District, Jiangsu Applicant before: Liu Hong |
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