CN105738575A - Anaerobic ammonia oxidation granular sludge shrinkage measuring device and method - Google Patents

Anaerobic ammonia oxidation granular sludge shrinkage measuring device and method Download PDF

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Publication number
CN105738575A
CN105738575A CN201610115342.7A CN201610115342A CN105738575A CN 105738575 A CN105738575 A CN 105738575A CN 201610115342 A CN201610115342 A CN 201610115342A CN 105738575 A CN105738575 A CN 105738575A
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cylinder
main
sludge
volume
reaction zone
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CN105738575B (en
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郑平
康达
胡倩怡
李旖瑜
叶贞
许少怡
张萌
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Zhejiang University ZJU
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Zhejiang University ZJU
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/28Anaerobic digestion processes
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials

Abstract

The invention discloses an anaerobic ammonia oxidation granular sludge shrinkage measuring device and method. The whole device comprises three parts, namely, a main body cylinder, a gas pressure balancer and a gas suction unit, wherein the main body cylinder is connected with the gas pressure balancer and the gas suction unit via a U-shaped pipe joint and a syringe joint on a threaded seal cover, and comprises the threaded seal cover, a gas collection zone and a reaction zone from the top down; the upper part of the reaction zone is a liquid layer, while the lower part of the reaction zone is a granular sludge layer; scales are marked on the side wall of the main body cylinder, and used for indicating change of the interface between the liquid layer and the granular sludge layer; the gas pressure balancer is provided with a small threaded seal cover and a liquid accommodating bottle, and used for sealing and exhausting of the main body cylinder; the gas suction unit is provided with a suction joint, a suction cylinder and a suction piston, and used for generating negative pressure and releasing gas in sludge. According to the device and the method, the shrinkage feature of granular sludge can be observed quantitatively, so as to indicate the activity and the settleability of the granular sludge; the structure is simple; the operation is convenient.

Description

Anaerobic ammonium oxidation granular sludge breathing determinator and method thereof
Technical field
The invention belongs to field of experiment equipment, be specifically related to a kind of anaerobic ammonium oxidation granular sludge breathing determinator and method thereof.
Background technology
Anammox be with ammonia be electron donor, nitrous acid for electron acceptor formed nitrogen biological respinse, ammonia and two kinds of pollutant of nitrous acid can be removed simultaneously, wastewater biological denitrificaion has tempting application prospect.Anaerobic ammonium oxidation granular sludge is the necessary basis of anaerobic ammonium oxidation sludge bed bioreactor Effec-tive Function, the activity of research and monitoring anaerobic ammonium oxidation granular sludge and settleability, contribute to understanding in real time the duty of anaerobic ammonium oxidation granular sludge, strengthen process control and the performance optimization of anaerobic ammonium oxidation sludge bed bioreactor.
Anaerobic ammonium oxidation granular sludge is made up of anaerobic ammonia oxidizing bacteria, Extracellular polymers (EPS) and hole, and substrate (ammonia and nitrous acid) enters inside granule sludge with water with the dissolved, is then changed into nitrogen by anaerobic ammonia oxidizing bacteria.Nitrogen dissolubility in water is relatively low, easily precipitates out and form bubble in granule sludge;Owing to the pore size in granule sludge is in capillary tube yardstick, bubble (nitrogen) easily " is blocked up " due to surface tension to be produced intrinsic pressure in hole, causes granule sludge volumetric expansion.When intrinsic pressure build up to exceed surface tension time, drive bubble to granule sludge apparent motion, when bubble migration to surface and when discharging, the intrinsic pressure rapid drawdown of granule sludge, volume contraction produces negative pressure, now sucks again water from granule sludge surface and is dissolved in substrate therein, starting next round breathing campaign.Owing to the breathing campaign of anaerobic ammonium oxidation granular sludge is caused by its internal microbial metabolism, and affected the settleability of granule sludge by granule sludge change in volume.Therefore, can be used for indicating the activity of granule sludge, amplitude of variation to can be used for indicating settling of granular sludge by the granule sludge change in volume cycle.But still do not have a kind of experimental facilities effectively can the breathing situation of anaerobic ammonium oxidation granular sludge be measured effectively at present, be unfavorable for that the research work of anaerobic ammonium oxidation granular sludge is carried out.
Summary of the invention
Present invention aim to address problems of the prior art, and a kind of anaerobic ammonium oxidation granular sludge breathing determinator is provided, by observing granule sludge volume dilational, indicate activity and the settleability of granule sludge.
To achieve these goals, the concrete technical scheme that the present invention adopts is as follows:
A kind of anaerobic ammonium oxidation granular sludge breathing determinator, device entirety is divided into main body cylinder, air pressure balancer and gas aspirator three part, specifically includes plenum zone, reaction zone, liquid level, granule sludge floor, major thread capping, the first U-tube joint, needle tube joint, U-tube, the second U-tube joint, liquid bottle, hydroecium, head space room, minor thread capping, exhaustor, air bleeding valve, suction joint, suction drums, pumping piston;
Main body barrel top is provided with major thread capping, and inside is followed successively by plenum zone and reaction zone from top to bottom;Top, reaction zone is liquid level, and bottom is granule sludge layer;Plenum zone is a head space, for collecting the gas that reaction zone produces and the negative pressure transmitting the generation of gas aspirator;Main body cylinder side wall indicates scale, may indicate that liquid level and the change of position, granule sludge bed boundary;Major thread capping is provided with U-tube joint and needle tube joint, and U-tube joint is by U-tube and air pressure balancer UNICOM, and needle tube joint is by aspirating joint and gas aspirator UNICOM;
Air pressure balancer is made up of minor thread capping and liquid bottle, is divided into head space room and hydroecium inside liquid bottle from top to bottom;Minor thread capping is provided with U-tube joint and exhaustor, U-tube joint by U-tube and main body cylinder UNICOM, exhaustor top and atmosphere and be provided with air bleeding valve;
Gas aspirator is provided with suction joint, suction drums and pumping piston;Suction joint is sand milling joint, bottom and needle tube joint sealed connection;Suction drums sidewall indicates scale, is used for indicating change in volume;Pumping piston is placed in suction drums, seals with sidewall but can twitch.
As preferably, in described main body cylinder, the ratio of reaction zone and plenum zone volume is 4:1;In reaction zone, liquid level is 1:2 with the ratio of granule sludge layer volume.
As preferably, in described air pressure balancer, in liquid bottle and main body cylinder, the ratio of reaction zone volume is 1:9;In liquid bottle, the ratio of head space room and hydroecium volume is 1:4;U-tube joint stretches into below the hydroecium water surface 20~30mm, for air-isolation.
As preferably, in described gas aspirator, in suction drums and main body cylinder, the ratio of plenum zone volume is 2:1.
Another object of the present invention is to provide a kind of anaerobic ammonium oxidation granular sludge breathing assay method using described device, step is as follows:
First culture fluid is put into the reaction zone of main body cylinder, records fluid level scale H1;Anaerobic ammonium oxidation granular sludge continues to put into reaction zone again, records fluid level scale H2;Difference H by two liquid levels2-H1As granule sludge initial volume;With major thread lidstock main body cylinder, open the air bleeding valve of air pressure balancer, pumping piston in gas aspirator is pushed into zero graduation;Anaerobic ammonium oxidation granular sludge is cultivated, fluid level scale H in main body cylinder when hydroecium no longer produces bubble in record liquid bottle3, the difference H of fluid level scale when main body cylinder fluid level scale now and granule sludge have just put into main body cylinder3-H2Volume is expanded for granule sludge;Closing the air bleeding valve of air pressure balancer, pump out the gas in main body cylinder with gas aspirator, make to produce in main body cylinder negative pressure, granule sludge discharges bubble, and record granule sludge discharges the main body cylinder fluid level scale H after bubble4;The difference H of fluid level scale when main body cylinder fluid level scale now and granule sludge have just put into main body cylinder2-H4For granule sludge shrinkage volume.
Further, granule sludge situation of drifting along in main body cylinder I and volume dilational change are associated, for the bubble volume allowed in quantitative Analysis anaerobic ammonium oxidation granular sludge.
The invention has the beneficial effects as follows: 1) device simple structure, easy to operate;2) by gas aspirator and air pressure balancer with the use of, the micro volume change of Observable granule sludge breathing;3) by the period of change of granule sludge volume, can determine whether granule sludge activity;4) by the amplitude of variation of granule sludge volume, can determine whether settling of granular sludge.
Accompanying drawing explanation
Fig. 1 is a kind of anaerobic ammonium oxidation granular sludge breathing assay device structures figure;
In figure: main body cylinder I, air pressure balancer II, gas aspirator III;Plenum zone 1, reaction zone 2, liquid level 3, granule sludge floor 4, major thread capping the 5, first U-tube joint 6, needle tube joint 7, U-tube the 8, second U-tube joint 9, liquid bottle 10, hydroecium 11, head space room 12, minor thread capping 13, exhaustor 14, air bleeding valve 15, suction joint 16, suction drums 17 and pumping piston 18.
Detailed description of the invention
Below in conjunction with concrete the drawings and specific embodiments, the present invention will be further described.If not having specified otherwise or conflict, each preferred implementation can be in any combination.
As it is shown in figure 1, a kind of anaerobic ammonium oxidation granular sludge breathing determinator.Device entirety is divided into main body cylinder I, air pressure balancer II and gas aspirator III tri-part.Specifically, including plenum zone 1, reaction zone 2, liquid level 3, granule sludge floor 4, major thread capping the 5, first U-tube joint 6, needle tube joint 7, U-tube the 8, second U-tube joint 9, liquid bottle 10, hydroecium 11, head space room 12, minor thread capping 13, exhaustor 14, air bleeding valve 15, suction joint 16, suction drums 17, pumping piston 18.
Main body cylinder I is used for placing mud granule, and its top is provided with dismountable major thread capping 5 and seals.Its inner space is followed successively by plenum zone 1 and reaction zone 2 from top to bottom.Under use state, top, reaction zone 2 is liquid level 3, and bottom is granule sludge layer 4.Plenum zone 1 is a head space, for collecting the gas that reaction zone 2 produces and the negative pressure transmitting gas aspirator III generation.Main body cylinder I sidewall indicates scale, may indicate that liquid level and the change of position, granule sludge bed boundary.Major thread capping 5 is provided with the U-tube joint 6 and needle tube joints 7 that connect outside in main body cylinder, and U-tube joint 6 is by U-tube 8 and air pressure balancer II UNICOM, and needle tube joint 7 is by aspirating joint 16 and gas aspirator III UNICOM.
Air pressure balancer II is made up of minor thread capping 13 and liquid bottle 10, and liquid bottle 10 is internal is divided into head space room 12 and hydroecium 11 from top to bottom;Minor thread capping 13 is provided with U-tube joint 9 and exhaustor 14, U-tube joint 9 by U-tube 8 and main body cylinder I UNICOM, exhaustor 14 top and atmosphere and be provided with air bleeding valve 15.
Gas aspirator III adopts the design of similar syringe, is provided with suction joint 16, suction drums 17 and pumping piston 18.Suction joint 16 be sand milling joint, bottom and needle tube joint 7 sealed connection;Suction drums 17 sidewall indicates scale, is used for indicating change in volume.Pumping piston 18 is placed in suction drums 17, seals with sidewall but can twitch by piston type.
Size and the visual practical situation of ratio of above-mentioned each device are set.In the present embodiment, in described main body cylinder I, the ratio of reaction zone 2 and plenum zone 1 volume is 4:1;In reaction zone 2, liquid level 3 is 1:2 with the ratio of granule sludge layer 4 volume.In described air pressure balancer II, in liquid bottle 10 and main body cylinder I, the ratio of reaction zone 2 volume is 1:9;In liquid bottle 10, the ratio of head space room 12 and hydroecium 11 volume is 1:4;U-tube joint 9 stretches into below hydroecium 11 water surface 20~30mm, for air-isolation.In described gas aspirator III, in suction drums 17 and main body cylinder I, the ratio of plenum zone 1 volume is 2:1.The test purpose of the present invention can be completed preferably through overtesting, above-mentioned size and ratio.
For the ease of aerogenesis and the situation of drifting along of Real Time Observation mud granule, described main body cylinder I adopts and makes transparent with machine glass;Described air pressure balance II can adopt Clear glass bottles and jars.
Based on said apparatus, present invention also offers a kind of anaerobic ammonium oxidation granular sludge breathing assay method, step is as follows:
First culture fluid is put into the reaction zone 2 of main body cylinder I, records fluid level scale H1;Anaerobic ammonium oxidation granular sludge continues to put into reaction zone 2 again, records fluid level scale H2;Difference H by two liquid levels2-H1As granule sludge initial volume;Cover 5 sealed body cylinder I with major thread, open the air bleeding valve 15 of air pressure balancer II, pumping piston 18 in gas aspirator III is pushed into zero graduation.After anaerobic ammonium oxidation granular sludge cultivates a period of time, air pressure balancer liquid bottle in hydroecium be can be observed and produce bubble, granule sludge activity size can be qualitatively judged according to bubble severe degree.In the incubation of anaerobic ammonium oxidation granular sludge, fluid level scale H in main body cylinder I when hydroecium 11 no longer produces bubble in record liquid bottle 103, the difference H of fluid level scale when main body cylinder fluid level scale now and granule sludge have just put into main body cylinder3-H2Volume is expanded for granule sludge;Closing the air bleeding valve 15 of air pressure balancer II, pump out the gas in main body cylinder I with gas aspirator III, make to produce in main body cylinder negative pressure, granule sludge discharges bubble, and record granule sludge discharges the main body cylinder fluid level scale H after bubble4;The difference H of fluid level scale when main body cylinder I fluid level scale now and granule sludge have just put into main body cylinder2-H4For granule sludge shrinkage volume.
It addition, in mensuration process, if associating granule sludge situation of drifting along in main body cylinder I and volume dilational change, for the bubble volume allowed in quantitative Analysis anaerobic ammonium oxidation granular sludge.Meanwhile, by the period of change of granule sludge volume, can determine whether granule sludge activity;By the amplitude of variation of granule sludge volume, can determine whether settling of granular sludge.Therefore, the present invention can the breathing feature of quantitative observation granule sludge, for indicating activity and the settleability of granule sludge, and there is simple structure, easy to operate advantage.

Claims (6)

1. an anaerobic ammonium oxidation granular sludge breathing determinator, it is characterized in that: device entirety is divided into main body cylinder (I), air pressure balancer (II) and gas aspirator (III) three part, specifically include plenum zone (1), reaction zone (2), liquid level (3), granule sludge layer (4), major thread capping (5), first U-tube joint (6), needle tube joint (7), U-tube (8), second U-tube joint (9), liquid bottle (10), hydroecium (11), head space room (12), minor thread capping (13), exhaustor (14), air bleeding valve (15), suction joint (16), suction drums (17), pumping piston (18);
Main body cylinder (I) top is provided with major thread capping (5), and inside is followed successively by plenum zone (1) and reaction zone (2) from top to bottom;Reaction zone (2) top is liquid level (3), and bottom is granule sludge layer (4);Plenum zone (1) is a head space, is used for collecting gas that reaction zone (2) produces and the negative pressure that transmission gas aspirator (III) produces;Main body cylinder (I) sidewall indicates scale, may indicate that liquid level and the change of position, granule sludge bed boundary;Major thread capping (5) is provided with U-tube joint (6) and needle tube joint (7), U-tube joint (6) is by U-tube (8) and air pressure balancer (II) UNICOM, and needle tube joint (7) is by aspirating joint (16) and gas aspirator (III) UNICOM;
Air pressure balancer (II) is covered (13) by minor thread and liquid bottle (10) forms, and liquid bottle (10) is internal is divided into head space room (12) and hydroecium (11) from top to bottom;Minor thread capping (13) is provided with U-tube joint (9) and exhaustor (14), U-tube joint (9) is by U-tube (8) and main body cylinder (I) UNICOM, exhaustor (14) top and atmosphere and is provided with air bleeding valve (15);
Gas aspirator (III) is provided with suction joint (16), suction drums (17) and pumping piston (18);Suction joint (16) is sand milling joint, bottom and needle tube joint (7) sealed connection;Suction drums (17) sidewall indicates scale, is used for indicating change in volume;Pumping piston (18) is placed in suction drums (17), seals with sidewall but can twitch.
2. anaerobic ammonium oxidation granular sludge breathing determinator according to claim 1, it is characterised in that: described main body cylinder (I) interior reaction zone (2) and the ratio of plenum zone (1) volume are 4:1;The ratio of reaction zone (2) interior liquid level (3) and granule sludge layer (4) volume is 1:2.
3. anaerobic ammonium oxidation granular sludge breathing determinator according to claim 1, it is characterised in that: described air pressure balancer (II) interior liquid bottle (10) and the ratio of main body cylinder (I) interior reaction zone (2) volume are 1:9;The ratio of the interior head space room (12) of liquid bottle (10) and hydroecium (11) volume is 1:4;U-tube joint (9) stretches into below hydroecium (11) water surface 20~30mm, for air-isolation.
4. anaerobic ammonium oxidation granular sludge breathing determinator according to claim 1, it is characterised in that: described gas aspirator (III) interior suction drums (17) and the ratio of main body cylinder (I) interior plenum zone (1) volume are 2:1.
5. the anaerobic ammonium oxidation granular sludge breathing assay method using device described in claim 1, it is characterised in that step is as follows:
First culture fluid is put into the reaction zone (2) of main body cylinder (I), records fluid level scale H1;Anaerobic ammonium oxidation granular sludge continues to put into reaction zone (2) again, records fluid level scale H2;Difference H by two liquid levels2-H1As granule sludge initial volume;Cover (5) sealed body cylinder (I) with major thread, open the air bleeding valve (15) of air pressure balancer (II), gas aspirator (III) interior pumping piston (18) is pushed into zero graduation;Anaerobic ammonium oxidation granular sludge is cultivated, fluid level scale H in the main body cylinder (I) when record liquid bottle (10) interior hydroecium (11) no longer produces bubble3, the difference H of fluid level scale when main body cylinder fluid level scale now and granule sludge have just put into main body cylinder3-H2Volume is expanded for granule sludge;Close the air bleeding valve (15) of air pressure balancer (II), the gas in main body cylinder (I) is pumped out with gas aspirator (III), make to produce in main body cylinder negative pressure, granule sludge discharges bubble, and record granule sludge discharges the main body cylinder fluid level scale H after bubble4;The difference H of fluid level scale when main body cylinder (I) fluid level scale now and granule sludge have just put into main body cylinder2-H4For granule sludge shrinkage volume.
6. anaerobic ammonium oxidation granular sludge breathing assay method as claimed in claim 5, it is characterised in that associate granule sludge situation of drifting along in main body cylinder I and volume dilational change, for the bubble volume allowed in quantitative Analysis anaerobic ammonium oxidation granular sludge.
CN201610115342.7A 2016-03-01 2016-03-01 Anaerobic ammonium oxidation granular sludge breathing determines devices and methods therefor Active CN105738575B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106248915A (en) * 2016-08-13 2016-12-21 赵东旭 Engineered cementitious concrete self-shrinkage measuring method and measurement apparatus

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CN104045156A (en) * 2014-06-20 2014-09-17 浙江大学 Integrated efficient autotrophic nitrogen removal reactor
CN204058059U (en) * 2014-07-17 2014-12-31 浙江大学 The bio-reactor of a kind of synchronous removal nitre nitrogen and ammonia nitrogen
CN205404538U (en) * 2016-03-01 2016-07-27 浙江大学 Anaerobic ammonium oxidation granular sludge breathing survey device

Patent Citations (6)

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Publication number Priority date Publication date Assignee Title
US20080006577A1 (en) * 2006-07-04 2008-01-10 Yong Su Choi Method and apparatus for wastewater treatment using nitrogen/phosphorous removal process combined with floatation separation of activated sludge
KR20110068697A (en) * 2009-12-16 2011-06-22 설동화 Sludge bulking improvement agent for biological treatment of sewage or waste water, and method for water treatment using the same
CN101863554A (en) * 2010-06-04 2010-10-20 浙江大学 Superhigh-efficiency anammox reactor
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106248915A (en) * 2016-08-13 2016-12-21 赵东旭 Engineered cementitious concrete self-shrinkage measuring method and measurement apparatus
CN106248915B (en) * 2016-08-13 2018-06-19 中交一公局桥隧工程有限公司 Engineered cementitious concrete self-shrinkage measuring method and measuring device

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