CN105717166A - Tree growth state real-time detecting device and method based on micro-fluidic chip - Google Patents
Tree growth state real-time detecting device and method based on micro-fluidic chip Download PDFInfo
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- CN105717166A CN105717166A CN201610084877.2A CN201610084877A CN105717166A CN 105717166 A CN105717166 A CN 105717166A CN 201610084877 A CN201610084877 A CN 201610084877A CN 105717166 A CN105717166 A CN 105717166A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
- G01N27/07—Construction of measuring vessels; Electrodes therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
Abstract
The invention discloses a tree growth state real-time detecting device and method based on a micro-fluidic chip, and relates to the microfluidics technology and the tree detecting technology.Millimeter-scale guide tubes are guided out of a main channel of the micro-fluidic chip, the guide-out ends are each fixedly connected with a needle, and the two needles are inserted into a tree guide tube vertically.Three detecting electrodes are arranged on one side of the upper portion of the main channel of the micro-fluidic chip in the axial direction of the main channel from top to bottom at equal intervals, a pressure detecting channel perpendicular to the main channel is guided out of the lower portion of the main channel, and a pressure sensor is arranged at an end opening of the pressure detecting channel.Liquid flows among the three detecting electrodes and a power supply electrode, one of the three detecting electrodes is selected to work through a circuit board, and ionic concentration is obtained.According to the method, the water amount in the tree guide tube can be detected in real time, the ionic concentration is detected through a conductivity method, data is accurate, whether the tree inorganic salt concentration is normal or not can be known, and detecting data is more precise and effective.
Description
Technical field
The present invention relates to microflow control technique and trees detection technique., specifically based on the real-time detection technique of arboreal growth state of micro-fluidic chip.
Background technology
In arboreal growth process, its internal moisture and inorganic salt concentration are the necessary key elements ensureing Health of Tree growth, just can well hold the situation of arboreal growth by detecting the two index.But at present detection mode to trees internal moisture and inorganic salt concentration is to first pass through artificial sample, more manually detect.This detection mode not only inefficiency, cost is high, and testing result lacks real-time, and data value is not high, and detection not even not timely causes negligence of management, affects arboreal growth.
At present, Chinese invention patent application number be 201520224038.7 document in propose a kind of arboreal growth state detector, the combine detection trees internal moisture and the pH value that use aqueous vapor detection module and PH detection module detect trees situation, detected by the aqueous vapor then passing through the conversion of trees internal moisture, so testing result can be produced extreme influence by the factor such as ambient temperature, humidity, data can not accurately represent the water yield within trees.The detection of this detector simultaneously is not related to the ion concentration of trees, have ignored the inorganic salt material impact to arboreal growth, to the detection of arboreal growth state insufficient.
Summary of the invention
It is an object of the invention to detect Problems existing for current arboreal growth, a kind of arboreal growth real-time detection device for state based on micro-fluidic chip and method are proposed, the detection water yield within trees and detection ion concentration in real time, understand whether trees inorganic salt concentration affects trees normal growth, testing result is accurate, simple in construction, processing ease and automaticity are high.
For achieving the above object, a kind of arboreal growth real-time detection device for state based on micro-fluidic chip of the present invention the technical scheme is that the fixing device including housing and being fixed on by housing on trees outer surface, enclosure interior is provided with circuit board and micro-fluidic chip, grade conduit is drawn from the main channel of micro-fluidic chip, each one syringe needle of fixing connection of exit, two syringe needles insert in the trees conduit in trees one on the other；Three detection electricity levels from top to bottom it are equidistantly positioned along the axial of main channel in the side, top, main channel of micro-fluidic chip, it is arranged below a current electrode at the 3rd detecting electrode, draw the pressure sensing channel vertical with main channel in bottom, main channel, the port at pressure sensing channel arranges a pressure transducer；Described three detection electricity levels and described pressure transducer are all connected to circuit board.
Housing arranges switch and USB2 interface, circuit board is by single chip part, usb interface module, self-detection module, A/D modular converter, Conductivity detection circuit, GSM message module and power supply form with switch module, single chip part respectively with usb interface module, self-detection module, A/D modular converter, GSM message module is connected with switch module with power supply, usb interface module connects USB interface, A/D modular converter connects Conductivity detection circuit and pressure transducer, Conductivity detection circuit is connected with current electrode and three detecting electrodes respectively, power supply and switch module connecting valve.
A kind of detection method based on the arboreal growth real-time detection device for state of micro-fluidic chip of the present invention employed technical scheme comprise that and comprises the following steps:
A, trees liquid by syringe needle along grade conduit enter micro-fluidic chip main channel along, pressure within micro-fluidic chip is transmitted circuit board by pressure transducer detection, and when pressure is abnormal, circuit board sends the abnormal information report of pressure, when pressure is normal, liquid continues flowing；
B, liquid flow through three between detecting electrode and current electrode, and circuit board selects one of them detecting electrode in three detecting electrodes, only receive the electrical conductivity of selected solution detected by electrode；
C, circuit board obtain ion concentration Ci according to formula L=A ∑ (Xi*Ci)/I, L is the electrical conductivity of liquid, and A is electrode area, and I is the vertical dimension between two electrodes, Ci is the concentration of ion i, Xi is the molar conductivity of ion i, by required ion concentration Ci compared with normal ion concentration range, if ion concentration Ci is in normal ion concentration range, circuit board does not send information report, liquid continues flowing, otherwise, then send information report.
Liquid flow through three between detecting electrode and current electrode time, the electrical conductivity L1 of solution, L2, L3 of being detected respectively by three detecting electrodes are sent to circuit board, if L1 < L2 < L3, L3-L1 < 10%*L1, select the 3rd detecting electrode measuring L3 as detecting electrode；If L1<L2<L3, L3-L1>10%*L1, L2-L1>10%*L1, being then error situation, remeasure after the interval detection time, if continuous 3 detection data are in error situation, then circuit board sends information report.
The invention have the advantages that
1, the present invention has only to be fixed on trees by device, insert the needle in trees conduit and can automatically work, liquid is taken by trees conduit is carried out syringe needle, the trees liquid flowing through micro-fluidic chip through conduit can be detected in real time, the water yield in detection trees conduit, it will be seen that whether trees the situation of hydropenia or water excess occur whereby, simple to operate and automaticity is high.
2, the present invention detects ion concentration with conductance method, and data are accurate, it will be appreciated that whether trees inorganic salt concentration is abnormal, affects trees normal growth.Preprocessing process is machine unification operation, decreases the detection noise that anthropic factor introduces.Employing multi-electrode designs, and is suitable for multiple trees detection 3, the present invention based on micro-fluidic chip, and by detecting the liquid in trees conduit thus detecting trees state in real time, detection data are more accurately effectively.
3, the present invention arranges GSM message module, has short message sending function, can be transmitted directly to user when detection goes wrong, easy to use, convenient efficiently.
4, syringe needle is connected trees place by the present invention rubber belt sealing, good airproof performance, can prevent syringe needle from coming off, impurity will not be introduced in trees.
Accompanying drawing explanation
Fig. 1 is the scheme of installation of a kind of arboreal growth real-time detection device for state based on micro-fluidic chip of the present invention；Fig. 2 be in Fig. 1 grade conduit 6 take liquid structure enlarged diagram；
Fig. 3 is Fig. 1 middle shell 4 internal structure enlarged diagram；
Fig. 4 is the internal structure of micro-fluidic chip 8 in Fig. 3 and outside installation connection diagram；
Fig. 5 is the fluid flow Cleaning Principle enlarged drawing of pressure transducer 14 in Fig. 4；
Fig. 6 is inside composition and the external connection enlarged diagram of circuit board 9 in Fig. 3.
The sequence number of each parts and title in accompanying drawing: 1. switch；2.USB interface；3. fixing device；4. housing；5. syringe needle；6. grade conduit；7. trees conduit；8. micro-fluidic chip；9. circuit board；10. the first detecting electrode；11. the second detecting electrode；12. the 3rd detecting electrode；13. current electrode；14. pressure transducer；15. micro-fluidic chip main channel；16. pressure sensing channel；17. single chip part；18.USB interface input module；19. self-detection module；20.A/D modular converter；21. Conductivity detection circuit；22.GSM SMS module；23. power supply and switch module；24. fixed hook；28. upper hole；29. lower opening；30. trees.
Detailed description of the invention
Referring to Fig. 1, a kind of arboreal growth real-time detection device for state based on micro-fluidic chip of the present invention by switching 1, USB interface 2, fixing device 3, housing 4 and grade conduit 6 constitute.Switch 1 and USB2 interface 2 are arranged on housing 4, and switch 1 is positioned at the middle part in housing 4 front, determines the opening and closing of whole device；USB interface 2 is positioned at the bottom in housing 4 front, makes user can revise device operating parameters.Fixing device 3 is connected to housing 4 left and right sides, and housing 4 is fixed on trees 30 outer surface by fixing device 3.The two ends up and down of grade conduit 6 are drawn from the both sides up and down of housing 4 accordingly, and it is internal that the two ends up and down that grade conduit 6 is drawn are all inserted into trees 30, are used for taking liquid and transmission liquid.
Referring to Fig. 2, two each one syringe needles 5 of fixing connection of exit up and down at grade conduit 6, syringe needle 5 is connected with grade conduit 6, two syringe needles 5 insert in the trees conduit 7 in trees 30 one on the other, with adhesive tape the junction good seal of syringe needle 5 and trees 30, trees liquid can pass through syringe needle 5 and be entered in housing 4 by grade conduit 6.Syringe needle 5 takes liquid technology maturation, ties up with adhesive tape and is prevented from liquid and spills.
Referring to Fig. 3, inside housing 4, it is provided with the micro-fluidic chip 8 of circuit board 9 and grade passage, in the micro-fluidic chip 8 embedded circuit plate 9 of grade passage.Switch 1 and USB interface 2 are all connected with circuit board 9.Respectively set a fixed hook 24 at the corner place of housing 4, catch on the corner of circuit board 9 with four fixed hooks 24, make circuit board 9 be stabilized in housing 4.Grade conduit 6 is drawn from the main channel of micro-fluidic chip 8, and good in junction solid gum adhesion.The lower opening 29 opened on upper hole 28 that grade conduit 6 is opened from housing 4 upper side and downside is drawn, with the fixing grade conduit 6 of adhesive tape, will not leakage when making to take liquid.
Referring to Fig. 4, grade conduit 6 is connected with the main channel 15 of micro-fluidic chip 8, and trees liquid enters in micro-fluidic chip 8 through grade conduit 6 and main channel 15, starts the detection to liquid in micro-fluidic chip 8.Three detection electricity levels are set in the side, top, main channel 15 of micro-fluidic chip 8, are the first detecting electrode the 10, second detecting electrode 11 and the 3rd detecting electrode 12 respectively, these three detection electricity level being axially from top to bottom equidistantly positioned along main channel 15.These three detection electricity level is partially in main channel 15.In the lower section of the 3rd detecting electrode 12 and be positioned at the opposite side of main channel 15 and arrange a current electrode 13.Before carrying out Conductivity detection, by the corresponding detecting electrode of procedure Selection, preserving detecting electrode position after choosing, detecting the electrical conductivity of this segment distance liquid with corresponding detecting electrode and current electrode, thus detecting the ion concentration of liquid.The micro-fluidic chip pressure sensing channel 16 vertical with main channel 15 is drawn in the bottom, main channel 15 of micro-fluidic chip 8, port at pressure sensing channel 16 arranges a pressure transducer 14, the induction end of pressure transducer 14 is connected with the port of pressure sensing channel 16, for measuring the pressure in pressure sensing channel 16, thus detecting fluid flow.Pressure sensing channel 16 and three detection electricity levels are positioned at the same side, and in the opposite side of current electrode 13, current electrode 13 is between pressure sensing channel 16 and the 3rd detecting electrode 12.First detecting electrode the 10, second detecting electrode the 11, the 3rd detecting electrode 12, current electrode 13 and pressure transducer 14 are all connected to circuit board 9.
Referring to Fig. 5, the sidewall of the main channel 15 of micro-fluidic chip 8 fixes Bonding pressure sense channel 16 one end, pressure sensing channel 16 other end Bonding pressure sensor 14, and pressure sensing channel 16 is perpendicular with main channel 15.The sensing part of pressure transducer 14 enters in pressure sensing channel 16.After trees liquid passes through the main channel 15 that grade conduit 6 enters micro-fluidic chip 8, owing to flow is different, the pressure of main channel 15 wall is also different, when flowing through pressure sensing channel 16, liquid can because pressure considerations enters pressure sensing channel 16, and the entrance of liquid can cause the pressure in pressure sensing channel 16 to change.Pressure transducer 14 is by detecting the pressure change in pressure sensing channel 16, thus the fluid flow can measured in trees conduit.Liquid is by, behind main channel 15, flowing out micro-fluidic chip 8 again through grade conduit 6 from top to bottom along main channel 15.
Referring to Fig. 6, the various functional modules on circuit board 9, the information analysis that the micro-fluidic chip 8 of grade passage is detected processes, and is sent to user further according to needs.Circuit board 9 is made up of single chip part 17, usb interface module 18, self-detection module 19, A/D modular converter 20, Conductivity detection circuit 21, GSM message module 22 and power supply and switch module 23.Single chip part 17 is arranged in the middle of circuit board 9, and single chip part 17 is connected with switch module 23 with usb interface module 18, self-detection module 19, A/D modular converter 20, GSM message module 22 and power supply respectively by different interfaces.Usb interface module 18 connects again USB interface 2, controls USB interface 2, it is possible to allow user pass through USB interface 2 and arrange parameters for transmission in single chip part 17.Self-detection module 19 is responsible for detecting whether whole device goes wrong.A/D modular converter 20 connects again Conductivity detection circuit 21, Conductivity detection circuit 21 is connected with first detecting electrode the 10, second detecting electrode the 11, the 3rd detecting electrode 12 and current electrode 13 respectively, utilizes first detecting electrode the 10, second detecting electrode the 11, the 3rd detecting electrode 12 and current electrode 14 to carry out Conductivity detection.A/D modular converter 20 is Bonding pressure sensor 14 again, and the analogue signal that Conductivity detection circuit 21 and pressure transducer 14 are detected converts digital signal to and is transmitted further to single chip part 17.GSM message module 22 is controlled by single chip part 17, and problem information is sent to user.Power supply and switch module 23 connecting valve 1, be responsible for the power supply of whole device, Conductivity detection circuit 21 and switch and 1 be connected, and switch 1 control power supply and switch module 23, power supply and switch module 23 are powered to single chip part 17 and as the power supply of Conductivity detection circuit 21.When switching 1 closedown, power supply and switch module 23 are no longer powered, and single chip part 17 cannot be run, and device quits work；When switch 1 is opened, single chip part 17 is started working, plant running.
With reference to Fig. 1-6, a kind of arboreal growth real-time detection device for state based on micro-fluidic chip of the present invention is when detecting in real time, first pass through USB interface 2 by various parameter input detection devices, it is transferred to single chip part 17 by usb interface module 18, for instance: the normal ion concentration range of the trees 30 of detection is Cmin ~ Cmax, is normal discharge scope, detection time T1, electrode area A, the vertical dimension I between the detecting electrode chosen and current electrode 13, the molar conductivity Xi etc. of ion i.Detecting device is placed on the appropriate location of trees 30, with fixing device 3, housing 4 is fixed, syringe needle 5 is inserted in trees conduit 7.Opening switch 1, start detecting device, power supply and switch module 23 are powered to detecting device.Detecting device can detect operation after starting automatically, and after user intends closedown detecting device, closing switch 1, switch 1 makes power supply and switch module 23 no longer power, and detecting device quits work.
After detecting device starts, the liquid of trees 30 is entered along grade conduit 6 by syringe needle 5 in the main channel 15 of micro-fluidic chip 8.When liquid carries out fluid flow detection through the pressure sensing channel 16 within micro-fluidic chip 8, analog data is converted to numerical data by the data that pressure transducer 14 detects A/D modular converter 20 in circuit board 9, is then forwarded to the single chip part 17 in circuit board 9.What detection data stored with single chip part 17 normal discharge scope compares, and when detection data, within the scope of normal discharge, photos and sending messages is not to user, and liquid continues flowing.If it exceeds or lower than normal discharge scope, carry out time delay detection, if detection time T1 in, detection data always abnormal, then the single chip part 17 in circuit board 9 indicate GSM message module 22 send note report user；If data recover normal in detection time T1, reexamining out of question, do not send note, detecting device continues detection, it has been found that carry out time delay detection during problem again.
Liquid is after flow detection, proceed by ion concentration detection, liquid flows through between first detecting electrode the 10, second detecting electrode the 11, the 3rd detecting electrode 12 and current electrode 13, owing to detecting device is just opened, first carry out electrode selection, select one of them detecting electrode in three detecting electrodes to work.After selecting, the single chip part in circuit board 9 17 receives the electrical conductivity L of the Conductivity detection circuit 21 solution by choosing electrode detection to arrive, and changes the data transmitted again through A/D modular converter 20, and the data of other transmission no longer receive.
First, Conductivity detection circuit 21 in circuit board 9 is carried out Conductivity detection using first detecting electrode the 10, second detecting electrode the 11, the 3rd detecting electrode 12 as detecting electrode respectively, the electrical conductivity L data obtained are changed through A/D modular converter 20, data are transmitted into single chip part 17.
Conductivity data respectively L1, L2, L3 that first detecting electrode the 10, second detecting electrode the 11, the 3rd detecting electrode 12 is measured.Assume L1 < L2 < L3, L3-L1 < 10%*L1, then circuit board 9 selects the 3rd detecting electrode 12 measuring L3 as detecting electrode.Namely, when the error between three data is below 10% (user can also when parameter be arranged, according to circumstances specification error scope), select to measure the maximum electrode of conductivity values as detecting electrode.
Assume L1<L2<L3, L3-L1>10%*L1, and L2-L1<10%*L1, then select the second detecting electrode 11 measuring L2 as detecting electrode.Namely when the error existed between 2 data is below 10%, one with other two ratios more than 10% time, in the Select Error 2 conductivity data below 10% numerical value bigger as detecting electrode.
Assume L1<L2<L3, L3-L1>10%*L1, L2-L1>10%*L1, be then error situation.Namely when three errors detected between data both are greater than 10%, the interval T1 time to remeasure, continuous 3 detection data are in this situation, and the single chip part 17 in circuit board 9 indicates GSM message module 22 to send problem by note and reports to user.
After detecting electrode is selected, fixes electrode position, no longer change afterwards, no longer carry out electrode selection.Single chip part 17 in circuit board 9 is according to formula: L=A ∑ (Xi*Ci)/I, obtains ion concentration Ci, and in formula, L is the electrical conductivity of liquid；A is electrode area；I is the vertical dimension between two electrodes；Ci is the concentration of ion i；Xi is the molar conductivity of ion i, then compares with the normal ion concentration range having stored in single chip part 17.If when detection data are in normal ion concentration range, namely during Cmin≤Ci≤Cmax, photos and sending messages is not to user, and liquid continues flowing；If it exceeds or<during Cmin, carry out time delay detection lower than normal ion concentration range Ci>Cmax or Ci.If being always up Ci within the T1 time>Cmax or Ci<Cmin, then single chip part 17 indicates GSM message module 22 to send note report user；If data recover normal Cmin≤Ci≤Cmax within the T1 time, reexamining out of question, do not send note, detecting device continues detection, it has been found that carry out time delay detection during problem again.Trees liquid after enter trees through grade conduit 6, the new liquid flowed into repeats said process.
Claims (7)
1. the arboreal growth real-time detection device for state based on micro-fluidic chip, including housing (4) and the fixing device (3) that housing (4) is fixed on trees outer surface, it is characterized in that: housing (4) is internal is provided with circuit board (9) and micro-fluidic chip (8), grade conduit (6) is drawn from the main channel (15) of micro-fluidic chip (8), exit one syringe needle (5) of each fixing connection, two syringe needles (5) are inserted in the trees conduit in trees one on the other；Three detection electricity levels from top to bottom it are equidistantly positioned along the axial of main channel (15) in main channel (15) side, top of micro-fluidic chip (8), it is arranged below a current electrode (13) at the 3rd detecting electrode, draw the pressure sensing channel (16) vertical with main channel (15) in main channel (15) bottom, the port at pressure sensing channel (16) arranges a pressure transducer (14)；Described three detection electricity levels and described pressure transducer (14) are all connected to circuit board (9).
2. a kind of arboreal growth real-time detection device for state based on micro-fluidic chip according to claim 1, it is characterized in that: housing (4) arranges switch (1) and USB2 interface (2), circuit board (9) is by single chip part (17) and the usb interface module (18) being connected with single chip part (17) respectively, self-detection module (19), A/D modular converter (20), Conductivity detection circuit (21), GSM message module (22) and power supply and switch module (23) form, usb interface module (18) connects USB interface (2), A/D modular converter (20) connects Conductivity detection circuit (21) and pressure transducer (14), Conductivity detection circuit (21) is connected with current electrode (13) and three detecting electrodes respectively, power supply and switch module (23) connecting valve (1).
3. a kind of arboreal growth real-time detection device for state based on micro-fluidic chip according to claim 1, it is characterized in that: in micro-fluidic chip (8) embedded circuit plate (9), respectively set a fixed hook (24) at the corner place of housing (4) and catch on the corner of circuit board (9).
4. a kind of arboreal growth real-time detection device for state based on micro-fluidic chip according to claim 1, is characterized in that: grade conduit (6) and described main channel (15) are tightly connected, and the junction of syringe needle (5) and trees seals.
5., as claimed in claim 1 based on an arboreal growth real-time detection device for state real-time detection method for micro-fluidic chip, it is characterized in that comprising the following steps:
A, trees liquid by syringe needle (5) along grade conduit (6) enter micro-fluidic chip (8) main channel (15) along, the pressure of micro-fluidic chip (8) inside is transmitted circuit board (9) by pressure transducer (14) detection, when pressure is abnormal, circuit board (9) sends the abnormal information of pressure, when pressure is normal, liquid continues flowing；
B, liquid flow through between three detecting electrodes and current electrode (13), and circuit board (9) selects one of them detecting electrode in three detecting electrodes, only receive the electrical conductivity of selected solution detected by electrode,
C, circuit board (9) obtain ion concentration Ci according to formula L=A ∑ (Xi*Ci)/I, L is the electrical conductivity of liquid, and A is electrode area, and I is the vertical dimension between two electrodes, Ci is the concentration of ion i, Xi is the molar conductivity of ion i, by required ion concentration Ci compared with normal ion concentration range, if ion concentration Ci is in normal ion concentration range, circuit board (9) does not send information report, liquid continues flowing, otherwise, then send information report.
6. real-time detection method according to claim 5, it is characterized in that: in step B, when liquid flows through between three detecting electrodes and current electrode (13), the electrical conductivity L1 of solution, L2, L3 of being detected respectively by three detecting electrodes are sent to circuit board (9), if L1 < L2 is < L3, L3-L1 < 10%*L1, selects the 3rd detecting electrode measuring L3 as detecting electrode.
7. real-time detection method according to claim 6, is characterized in that: if L1<L2<L3, L3-L1>10%*L1, L2-L1>10%*L1, then be error situation, remeasure after the interval detection time, if continuous 3 detection data are in error situation, then circuit board (9) sends information report.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110579518A (en) * | 2019-09-12 | 2019-12-17 | 江南大学 | Tea polyphenol electrochemical detection device based on vertical mixing micro-fluidic chip |
| CN114858980A (en) * | 2022-05-19 | 2022-08-05 | 景德镇学院 | Device and method for detecting tree growth state in real time based on micro-fluidic chip |
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| CN114858980A (en) * | 2022-05-19 | 2022-08-05 | 景德镇学院 | Device and method for detecting tree growth state in real time based on micro-fluidic chip |
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| CN105717166B (en) | 2019-08-27 |
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