CN105674881A - Plant stem measuring method and device - Google Patents
Plant stem measuring method and device Download PDFInfo
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- CN105674881A CN105674881A CN201610050946.8A CN201610050946A CN105674881A CN 105674881 A CN105674881 A CN 105674881A CN 201610050946 A CN201610050946 A CN 201610050946A CN 105674881 A CN105674881 A CN 105674881A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
- G01B11/022—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness by means of tv-camera scanning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/08—Measuring arrangements characterised by the use of optical techniques for measuring diameters
Abstract
The invention discloses a plant stem measuring method. The method comprises that S1) an image of a plant sample to be measured is collected; S2) an in-situ image of the plant sample to be measured is obtained according to the collected image; and S3) the in-situ image is processed to obtain image data, and a phenotypic characteristic parameter of a stem of the plant sample to be measured is calculated according to the image data. According to the invention, the in-situ image of the plant stem is obtained, and processed and analyzed to obtain the phenotypic characteristic parameter of the plant stem, the in-situ image of the plant stem can be obtained scientifically without loss, the sample is effectively prevented from dehydration, wilting and further deformation in the sampling process, and it is ensured that the plant stem is avoided from influence on growth due to being broken.
Description
Technical field
The present invention relates to agricultural biological technical field, particularly relate to a kind of plant haulm measuring method and device.
Background technology
Stem stalk is not only growth conveying nutriment and the moisture of plant, and support blade and flowers and fruits, directly determine the spatial distribution of each organ, be most important characters with plant. Stalk characters includes that plant height, stem be thick, the number of volume, joint, panel length and branch amount etc. The growth of stem stalk is closely related with growth and plant lodging tolerance. Plant lodging is one of key factor affecting Semen Tritici aestivi, Oryza sativa L., seeding corn and other crops high yield, and in high-yield breeding of crops, the lodging resistance traits improving crop stem is an important breeding deliberated index, and the genetic breeding of crop is particularly significant.
The measurement of stem stalk correlated traits, at present still based on manual measurement, in conjunction with conventional tool such as ruler, tape measure, slide gaugies, obtains including the stem stalk phenotypic characteristic parameter of plant height, panel length, the number of joint, branch amount. Being measured as example with plant height, with ruler or tape measure from plant base portion, to plant top, the data obtained is plant height. Can carrying out, with slide gauge, the measurement that stem is thick, the measurements such as culm length, panel length, stem are thick can also be analyzed by some image processing softwares such as ImageJ.
At present, in plant stem determination techniques, domestic and international experts and scholars are mainly also dependent on manual measurement. This method can bring relatively larger personal error, and would generally bring unnecessary damage to tissues such as plant stems, and when particularly high flux is measured, consuming time longer, efficiency is on the low side. In the process measuring plant height, having one critical to technical problem is that plant base portion is near soil, many times survey tool is difficult to accurately measure the initiation site of base portion, and corresponding plant height measured value has error. The measurement of panel length is also required to similar method, and a lot of plant joint is a lot, and corresponding panel length to be measured several times and to obtain, and process is loaded down with trivial details, and personal error is very big. Particularly running into and need to analyze large batch of plants stems stalk correlated traits, when will carry out statistical analysis, manually will record each measured value one by one, workload is very big.The Morphological Modeling that other is relevant to stem stalk, it is necessary to phenotype is measured instrument auxiliary and carried out. And image processing software method all needs plant is carried out sampling analysis, inevitably plant being caused damage, picture processing is also required to manual operation in this process, the error brought is also very big. It addition, for the volume of stem stalk, skeleton, three-dimensional configuration modeling etc., but without good measuring method, be difficult to there is an accurate measurement technology, limit technical staff's research to plant haulm.
Summary of the invention
It is an object of the invention to provide a kind of plant haulm measuring method and device, accurately can not only nondestructively obtain and show the growth promoter state of plant haulm, it is also possible to the various phenotypic characteristic parameters of plants stems stalk are included skeleton and three-dimensional configuration modeling measurement and analyze.
Technical scheme provided by the invention is as follows:
The invention discloses a kind of plant haulm measuring method, including: plant sample to be measured is carried out image acquisition by S1; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculates the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
In-situ image, by obtaining the in-situ image of plant stem, is processed and analyzes the phenotypic characteristic parameter drawing plant stem by the present invention, it is possible to the in-situ image obtaining plant stem that science is lossless completely, will not bring damage to plant.
The invention also discloses another kind of plant haulm measuring method, including: S0 RF identification plant sample to be measured, obtain the numbering of plant sample to be measured; Plant sample to be measured is carried out image acquisition by S1; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculates the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The present invention treats measuring plants be numbered by arranging electronic tag on the flowerpot treat measuring plants, obtains each numbering treating measuring plants again through RF identification, it is simple to the image of different plants is processed by the later stage.
The invention also discloses another kind of plant haulm measuring method, including: S1 adopts visible ray image side faces method that plant sample to be measured is carried out image acquisition, plant sample to be measured is carried out image acquisition and includes by described step S1, adopts visible ray image side faces method that plant sample to be measured is carried out image acquisition; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculates the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The present invention is that the stem stalk to plant carries out imaging analysis, so adopting visible ray image side faces, through automatic conveyor, plant being sent to 3D imaging region, chooses object of reference, it is simple to the correction between pixel and physical length.
The invention also discloses another kind of plant haulm measuring method, including: plant sample to be measured is carried out image acquisition by S1; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculate the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data, the phenotypic characteristic parameter that described step S3 calculates plant sample stem stalk to be measured according to described view data specifically includes: obtain the pixel value of stem stalk phenotypic characteristic parameter according to described view data;Pixel value according to described stem stalk phenotypic characteristic parameter and the object of reference pixel pre-set calculate the phenotypic characteristic parameter that stem stalk is actual.
The present invention is based on the technology of calculation visualization, by to in-situ image Treatment Analysis, immediately arrive at the pixel value of the phenotypic characteristic parameter of plant haulm, it is corrected by referring to thing, is converted to physical length, it is thus achieved that actual characteristic feature parameter, the present invention can farthest reduce the error that manual measurement brings, what measurement result was formed is visual image, can intuitively and accurately obtain the phenotypic characteristic parameter of stem stalk on this image, the scientific aspect of data is had great lifting.
It is further preferred that the in-situ image of described plant sample to be measured is multiple.
Selected multiple original position pictures can be carried out batch quantity analysis by the present invention, rapidly be the various phenotypic characteristic parameters of plant haulm after batch processing, and then conveniently carry out next step statistical analysis, the present invention is applicable to high-throughout plant haulm phenotypic characteristic and analyzes, and improves the work efficiency that stem stalk is measured.
The invention also discloses a kind of plant haulm measurement apparatus, including: image capture module, for plant sample to be measured is carried out image acquisition; Image collection module, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module, obtains view data for carrying out described in-situ image processing; Computing module, for calculating the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The invention also discloses another kind of plant haulm measurement apparatus, including: radio frequency identification module, for RF identification plant sample to be measured, obtain the numbering of plant sample to be measured; Image capture module, for carrying out image acquisition to plant sample to be measured; Image collection module, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module, obtains view data for carrying out described in-situ image processing; Computing module, for calculating the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The invention also discloses another kind of plant haulm measurement apparatus, including: image capture module, specifically for adopting visible ray image side faces method that plant sample to be measured is carried out image acquisition; Image collection module, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module, obtains view data for carrying out described in-situ image processing; Computing module, for calculating the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The invention also discloses another kind of plant haulm measurement apparatus, including: image capture module, for plant sample to be measured is carried out image acquisition; Image collection module, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module, obtains view data for carrying out described in-situ image processing; Computing module, specifically for the described pixel value obtaining stem stalk phenotypic characteristic parameter according to described view data, calculates, according to the pixel value of described stem stalk phenotypic characteristic parameter and the object of reference pixel pre-set, the phenotypic characteristic parameter that stem stalk is actual.
Compared with prior art, the present invention can bring at least one beneficial effect following:
1) present invention is without manual measurement, can obtain each phenotypic parameter of plant haulm, not only save manual sampling, measure, data inputting, the time and efforts of statistical analysis, original position and the integrity of plant, lossless acquisition stem stalk phenotypic information can also be ensured.
2) farthest reducing the error that manual measurement brings, what measurement result was formed is visual image, can intuitively and accurately obtain the phenotypic parameter of stem stalk on this image.
3) the phenotypic characteristic parameter of plant haulm can be obtained from different perspectives, and then plant haulm growth promoter state can be disclosed from 3D aspect. Help researcher and more comprehensively understand stem stalk phenotypic characteristic.
4) selected picture can be carried out batch quantity analysis, rapidly be the various phenotypic characteristic parameters of plant haulm after batch processing, and then conveniently carrying out next step statistical analysis, it is adaptable to high-throughout plant haulm phenotypic characteristic is analyzed, and improves the work efficiency that stem stalk is measured.
Accompanying drawing explanation
Below by the way of clearly understandable, accompanying drawings preferred implementation, a kind of plant haulm measuring method and the above-mentioned characteristic of device, technical characteristic, advantage and implementation thereof are further described.
Fig. 1 is the key step schematic diagram of a kind of plant haulm measuring method of the present invention;
Fig. 2 is the schematic diagram of a specific embodiment of a kind of plant haulm measuring method of the present invention;
Fig. 3 is the schematic diagram of another specific embodiment of a kind of plant haulm measuring method of the present invention;
Fig. 4 is the composition structural representation of a kind of plant haulm measurement apparatus of the present invention.
Drawing reference numeral illustrates:
100. image capture module, 200. image collection module, 300. image processing modules, 400. computing modules.
Detailed description of the invention
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below comparison accompanying drawing is illustrated the specific embodiment of the present invention. Apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the premise not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings, and obtain other embodiment.
For making simplified form, only schematically show part related to the present invention in each figure, they do not represent its practical structures as product. It addition, so that simplified form readily appreciates, some figure has the parts of identical structure or function, only symbolically depict one of them, or only mark one of them. In this article, " one " not only represents " only this ", it is also possible to represent the situation of " more than one ".
Fig. 1 is the key step schematic diagram of a kind of plant haulm measuring method of the present invention, as a specific embodiment of the present invention, as it is shown in figure 1, a kind of plant haulm measuring method, including: plant sample to be measured is carried out image acquisition by S1; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculates the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The present invention is by the in-situ image according to the Image Acquisition plant stem gathered, in-situ image processes and analyzes the phenotypic characteristic parameter drawing plant stem, can completely science lossless obtain plant stem in-situ image, sample dehydration in stem stalk sampling process is effectively avoided to wilt, the phenomenons such as the deformation causing stem stalk, ensure to measure and plant haulm is not resulted in problems such as fractureing, affect the g and D of plants stems stalk.
Concrete, the present invention can also obtain the phenotypic parameter of plant haulm from different perspectives, solid geometric pattern is built based on above-mentioned phenotypic characteristic parameter, and carry out visualization of 3 d reconstruction further, build the three-dimensional stereo model of cane, and then plant haulm growth promoter state can be disclosed from 3D aspect, simultaneously, utilize the new algorithm continually developed, obtain more geometric configuration relevant parameter, help researcher and more comprehensively understand stem stalk phenotypic characteristic.
Above-described embodiment is improved, obtains another kind of plant haulm measuring method, including: S0 RF identification plant sample to be measured, obtain the numbering of plant sample to be measured;Plant sample to be measured is carried out image acquisition by S1; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculates the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The present invention treats measuring plants be numbered by arranging electronic tag on the flowerpot treat measuring plants, obtains each numbering treating measuring plants again through RF identification, it is simple to the image of different plants is processed by the later stage.
Above-described embodiment is improved, obtain another kind of plant haulm measuring method, including: plant sample to be measured is carried out image acquisition by S1, plant sample to be measured is carried out image acquisition and includes by described step S1, adopts visible ray image side faces method that plant sample to be measured is carried out image acquisition; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculates the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
The present invention is that the stem stalk to plant carries out imaging analysis, so adopting visible ray image side faces, through automatic conveyor, plant being sent to 3D imaging region, chooses object of reference, it is simple to the correction between pixel and physical length.
Above-described embodiment is improved, obtain another kind of plant haulm measuring method, including: plant sample to be measured is carried out image acquisition by S1, plant sample to be measured is carried out image acquisition and includes by described step S1, adopts visible ray image side faces method that plant sample to be measured is carried out image acquisition; The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered; Described in-situ image is carried out processing acquisition view data by S3, calculate the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data, the phenotypic characteristic parameter that described step S3 calculates plant sample stem stalk to be measured according to described view data specifically includes: obtain the pixel value of stem stalk phenotypic characteristic parameter according to described view data; Pixel value according to described stem stalk phenotypic characteristic parameter and the object of reference pixel pre-set calculate the phenotypic characteristic parameter that stem stalk is actual.
Concrete, the process of in-situ image is included: by in-situ image demosaicing, coloured image is changed into greyish white image, set gray threshold, set filter, set transducer, set survey region, from the selected object of study of survey region, according to object of reference correction chart picture, export view data.
The present invention is based on the technology of calculation visualization, by in-situ image Treatment Analysis, immediately arriving at the pixel value of the phenotypic characteristic parameter of plant haulm, it is corrected by referring to thing, be converted to physical length, it is thus achieved that actual characteristic feature parameter, export view data. The present invention can farthest reduce the error that manual measurement brings, and what measurement result was formed is visual image, can intuitively and accurately obtain the phenotypic characteristic parameter of stem stalk on this image, the scientific aspect of data is had great lifting.
Preferably, the in-situ image of described plant sample to be measured is multiple.
The present invention can in same IAC preserves and arranges, selected multiple in-situ images are carried out batch quantity analysis, rapidly be the various phenotypic characteristic parameters of plant haulm after batch processing, and then conveniently carry out next step statistical analysis, the present invention is applicable to high-throughout plant haulm phenotypic characteristic and analyzes, and improves the work efficiency that stem stalk is measured.
The method adopting the present invention, it is possible to the plant height of Semen Maydis, panel length, internode number, stem be thick and Semen Maydis skeleton isophenous characteristic parameter for analyzing.Fig. 2 is the schematic diagram of a specific embodiment of a kind of plant haulm measuring method of the present invention, as in figure 2 it is shown, be followed successively by Semen Maydis in-situ image, Semen Maydis skeleton drawing, stem stalk blade segmentation figure from left to right.
By color converter, threshold value setting and skeleton analysis etc., the in-situ image of Semen Maydis is carried out image side faces analysis, in conjunction with object of reference by the pixel unit of gained, convert through conversion coefficient, obtain the actual plant height of Semen Maydis, panel length, the thick isophenous characteristic parameter of stem.
Based on this, it is possible to the in-situ image of batch processing Semen Maydis, the high flux data of the geometric configuration of corn stem are obtained.
The method adopting the present invention, it is possible to analyze the various geometric configurations of different cultivars Fructus Lycopersici esculenti plant type. Fig. 3 is the schematic diagram of another specific embodiment of a kind of plant haulm measuring method of the present invention. As it is shown on figure 3, be the geometric configuration measurement of two kinds of tomato plants stem stalks, it is possible to original position obtains the parameters such as maximum circumscribed circle area, maximum rectangular area, minimum rectangle area, it is possible to the lossless geometric configuration analyzing plant haulm.
Fig. 4 is the composition structural representation of a kind of plant haulm measurement apparatus of the present invention, as a specific embodiment of the present invention, as shown in Figure 4, a kind of plant haulm measurement apparatus, including: image capture module 100, for plant sample to be measured is carried out image acquisition; Image collection module 200, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module 300, obtains view data for carrying out described in-situ image processing; Computing module 400, for calculating the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
Above-described embodiment is improved, obtains another kind of plant haulm measurement apparatus, including: radio frequency identification module, for RF identification plant sample to be measured, obtain the numbering of plant sample to be measured; Image capture module 100, for carrying out image acquisition to plant sample to be measured; Image collection module 200, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module 300, obtains view data for carrying out described in-situ image processing; Computing module 400, for calculating the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
Above-described embodiment is improved, obtains another kind of plant haulm measurement apparatus, including: image capture module 100, specifically for adopting visible ray image side faces method that plant sample to be measured is carried out image acquisition; Image collection module 200, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module 300, obtains view data for carrying out described in-situ image processing; Computing module 400, for calculating the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
Above-described embodiment is improved, obtains another kind of plant haulm measurement apparatus, including: image capture module 100, for plant sample to be measured is carried out image acquisition; Image collection module 200, for the in-situ image according to the Image Acquisition plant sample to be measured gathered; Image processing module 300, obtains view data for carrying out described in-situ image processing; Computing module 400, specifically for the described pixel value obtaining stem stalk phenotypic characteristic parameter according to described view data, calculates, according to the pixel value of described stem stalk phenotypic characteristic parameter and the object of reference pixel pre-set, the phenotypic characteristic parameter that stem stalk is actual.
It should be noted that, above-described embodiment all can independent assortment as required.The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention; can also making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (9)
1. a plant haulm measuring method, it is characterised in that including:
Plant sample to be measured is carried out image acquisition by S1;
The S2 in-situ image according to the Image Acquisition plant sample to be measured gathered;
Described in-situ image is carried out processing acquisition view data by S3, calculates the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
2. plant haulm measuring method as claimed in claim 1, it is characterised in that also include before described step S1:
S0 RF identification plant sample to be measured, obtains the numbering of plant sample to be measured.
3. plant haulm measuring method as claimed in claim 1, it is characterised in that plant sample to be measured is carried out image acquisition and includes by described step S1, adopts visible ray image side faces method that plant sample to be measured is carried out image acquisition.
4. plant haulm measuring method as claimed in claim 1, it is characterised in that the phenotypic characteristic parameter that described step S3 calculates plant sample stem stalk to be measured according to described view data specifically includes:
The pixel value of stem stalk phenotypic characteristic parameter is obtained according to described view data;
Pixel value according to described stem stalk phenotypic characteristic parameter and the object of reference pixel pre-set calculate the phenotypic characteristic parameter that stem stalk is actual.
5. plant haulm measuring method as claimed in claim 1, it is characterised in that the in-situ image of described plant sample to be measured is multiple.
6. a plant haulm measurement apparatus, it is characterised in that including:
Image capture module, for carrying out image acquisition to plant sample to be measured;
Image collection module, for the in-situ image according to the Image Acquisition plant sample to be measured gathered;
Image processing module, obtains view data for carrying out described in-situ image processing;
Computing module, for calculating the phenotypic characteristic parameter of plant sample stem stalk to be measured according to described view data.
7. plant haulm measurement apparatus as claimed in claim 6, it is characterised in that also include:
Radio frequency identification module, for RF identification plant sample to be measured, obtains the numbering of plant sample to be measured.
8. plant haulm measurement apparatus as claimed in claim 6, it is characterised in that plant sample to be measured is carried out image acquisition specifically for adopting visible ray image side faces method by described image capture module.
9. plant haulm measurement apparatus as claimed in claim 6, it is characterized in that, described computing module, specifically for obtaining the pixel value of stem stalk phenotypic characteristic parameter according to described view data, calculates, according to the pixel value of described stem stalk phenotypic characteristic parameter and the object of reference pixel pre-set, the phenotypic characteristic parameter that stem stalk is actual.
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| CN106323182A (en) * | 2016-08-08 | 2017-01-11 | 北京农业信息技术研究中心 | Plant stalk diameter measuring method and device |
| CN108694727A (en) * | 2018-04-17 | 2018-10-23 | 南京农业大学 | A kind of miniature multiple spot Root morphology acquisition and processing system in real time |
| CN110163854A (en) * | 2019-05-16 | 2019-08-23 | 北京农业信息技术研究中心 | Corn internode phenotypic parameter acquisition methods and device |
| CN110455201A (en) * | 2019-08-13 | 2019-11-15 | 东南大学 | Stalk plant height measurement method based on machine vision |
| CN111179363A (en) * | 2020-01-14 | 2020-05-19 | 北京农业信息技术研究中心 | High-throughput plant phenotype measuring device and method |
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